Entries |
Document | Title | Date |
20080213816 | Mitochondrial enoyl coenzyme a hydratase 1 as marker for diagnosing stomach cancer - The present invention relates to mitochondrial protein that can be used as a marker for diagnosing stomach cancer. According to the present invention, the marker for diagnosing stomach cancer comprises mitochondrial enoyl coenzyme A hydratase 1. | 09-04-2008 |
20080227135 | Autoimmune Conditions and NADPH Oxidase Defects - The invention relates to methods and materials involved in diagnosing and treating autoimmune conditions. In particular, the invention relates to methods and materials involved in diagnosing arthritis conditions that are accompanied by an NADPH oxidase deficiency, methods and materials involved in treating, preventing, or delaying the onset of arthritis conditions that are accompanied by an NADPH oxidase deficiency, and methods and materials involved in identifying agonists and antagonists of NADPH oxidase activity. | 09-18-2008 |
20080233606 | USE OF RAMAN SPECTROSCOPY IN ENZYME ACTIVITY ASSAYS - Provided herein are assays for detecting enzyme activity using Raman Spectroscopy. | 09-25-2008 |
20080254495 | Method of Diagnosing Apoplectic Stroke/Asymptomatic Brain Infarction Using Polyamine and Acrolein Contents, Polyamine Oxidase Activity or Protein Content Thereof as Indication - The present invention provides a diagnostic method for stroke/asymptomatic cerebral infarction and a screening method for patients with stroke/asymptomatic cerebral infarction, which comprise measuring acrolein content or polyamine content; or polyamine oxidase activity or protein content of polyamine oxidase in plasma. The knowledge of the present invention indicates the possibility of preventing, inhibiting the progression of stroke/asymptomatic cerebral infarction by inhibiting polyamine oxidase, and the possibility of obtaining a therapeutic agent for stroke/asymptomatic cerebral infarction by searching for compounds that inhibit polyamine oxidase. | 10-16-2008 |
20080254496 | Methods for Detection of Fungal Disease - The present invention describes the use of enzymes having D-arabinitol oxidase activity with the concomitant generation of hydrogen peroxide for the diagnosis of fungal infections of humans and other organisms, and for the detection of fungal organisms that are present on or in surfaces or permeable materials. The present invention also describes methods and kits for the detection of fungi, and the diagnosis of fungal infections based upon D-arabinitol oxidase activity. The present invention also provides a new biochemical activity for gene products encoded by bacterial genes previously identified only with putative function. Activity of these gene products includes the ability to oxidize D-arabinitol with the concomitant generation of hydrogen peroxide. | 10-16-2008 |
20080268488 | Screening Method for Dnak Inhibitors - Screening methods for identification of inhibitors of DnaK activity are provided. Such inhibitors have utility as antibacterial agents. | 10-30-2008 |
20080305511 | Method for screening antioxidant using mutant bacteria and chlorophyllide, and antioxidant screened by the method - Disclosed is a method for screening an antioxidant using mutant bacteria and chlorophyllide, and an antioxidant screened by the same method. More specifically, provided is a method for easy screening of an antioxidant by monitoring growth profiles of specific mutant bacteria in filter discs or medium blocks containing chlorophyllide added thereto, and an antioxidant screened by the same method. | 12-11-2008 |
20090029400 | Pharmaceuticals and methods for treating hypoxia and screening methods therefor - Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions. | 01-29-2009 |
20090042232 | MUTANT PROTEIN HAVING DIAPHORASE ACTIVITY - A mutant diaphorase capable of having lower potential energy than that of wild-type diaphorase of sequence ID No. 1 when forming a complex with a coenzyme, flavin mononucleotide. | 02-12-2009 |
20090061471 | Methods and systems for selective fluorination of organic molecules - A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity. | 03-05-2009 |
20090075319 | Method of Detecting Reactive Metabolite - A method of detecting a reactive metabolite which comprises steps of conducting a metabolic reaction of a test compound in the presence of two labeled nucleophiles, and detecting the label of the nucleophiles bound to the reactive metabolite, and a method of detecting a reactive metabolite which further comprises separating liquid layer using ammonium acetate to obtain an organic layer after the aforementioned reaction step are disclosed. In these detection methods, a reactive metabolite can be quantitatively detected by easy preparation. Furthermore, generation of a false positive signal can be prevented, and generation of a false negative signal can be decreased. Accordingly, screening of a test compound can be efficiently performed with higher accuracy. | 03-19-2009 |
20090087872 | ASSAY FOR IN VITRO TESTING OF ENZYME CATALYTIC ACTIVITY - The invention provides a method of evaluating metabolism-based drug interactions. The method involves selecting time points for the determination of the inactivation rate constant of a time-dependent enzyme inhibitor based on the results of a multi-time point IC50 test. Advantageously, with the subject invention, the determination and use of the multi-time point IC50 test provides an indication of the inactivation rate of a test compound and eliminates trial and error tests associated with the selection of appropriate assay conditions for the second assay conducted to determine the inactivation rate constant of the test compound. | 04-02-2009 |
20090098592 | METHOD - A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form. | 04-16-2009 |
20090123956 | Measurement of the oxidants-antioxidants balance in liquids - The present invention describes a chemical method that can determine the oxidant-antioxidant balance in biological samples and other materials. In the example of the application of the invention that is presented in the description of the invention section 3,3′,5,5′-Tetramethylbenzidine (TMB) and its cation that has a characteristic color are used as an oxidation-reduction target. However any other substance that can change its optical, fluorescence luminescence properties upon oxidation or reduction could be used in its place. The invention can be applied in any shape of vessel and on a stable matrix as a dipstick. The invention is based on two reactions one redox and one enzymatic that take place at the same time. In a redox reaction, e.g. TMB cation will be reduced by antioxidants; in the enzymatic reaction, intact TMB will be oxidized by peroxides. In the process of reduction, TMB cation will be decolourized; and in the process of oxidation, intact TMB will be converted to a colour cation. After a period of the time and adding HCl, the amount of TMB cation can be easily measured by spectrophotometry at 450 nm (reference wavelength 620 or 570 nm) both by macro- and micromethods (ELISA reader). The quantitative amount of TMB cation is representative of the oxidants-antioxidants balance in sample. This is achieved by comparing the optical absorbance of each sample with the absorbance of a series of standards that comprise the standard curve. The standard solutions can be constructed by mixing varying proportions (0-100%) of hydrogen peroxide (as a representative of the oxidants) with uric acid (as a representative of the antioxidants). However the admixture of any other oxidant-antioxidant may be used. The invention can be useful for the evaluation of the oxidant-antioxidant balance in biological samples (serum, plasma, urine etc.) especially for the evaluation of age related and metabolic disease such as diabetes. In addition the invention may be useful for the estimation of the success of antioxidant therapy. | 05-14-2009 |
20090123957 | CANINE COX-2 PROTEINS AND USES THEREOF - The present invention relates to canine COX-1 and COX-2 proteins; to canine COX-1 and COX-2 nucleic acid molecules, including those that encode such COX-1 and COX-2 proteins, respectively; to antibodies raised against such proteins; and to compounds that inhibit the activity of such proteins. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. The present invention also includes therapeutic compositions comprising such inhibitory compounds, particularly those that specifically inhibit COX-2 activity, as well as the use of such therapeutic compositions to treat animals. | 05-14-2009 |
20090148878 | Secreted and transmembrane polypeptides and nucleic acids encoding the same - The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. | 06-11-2009 |
20090162884 | Method of disulfide crosslink forming in vitro protein synthesis - It is an objective of the present invention to provide a method of synthesizing a protein having disulfide bonds using a reconstituted protein synthesizing system in a convenient manner with high efficiency. It has been found that a protein having the activity can be obtained and synthesized with good efficiency by using a reconstituted protein synthesizing system comprising purified components, from which enzymes and substrates influencing an oxidation-reduction state are removed and in which a state of redox equilibrium between disulfide and thiol is artificially regulated. Such system is used instead of a cell extract or a crude fraction thereof that contains various enzymes and substrates that maintain a reduced state, such as thioredoxin reductase [EC 1.6.4.5] and glutathion reductase [EC 1.6.4.2], resulting in difficulty in conditioning a redox state. | 06-25-2009 |
20090208994 | GENETIC INCORPORATION OF 3-AMINOTYROSINE INTO REDUCTASES - This invention provides reductase proteins that comprise NH | 08-20-2009 |
20090208995 | PRO-CLOTTING ENZYME, AND METHOD FOR DETECTION OF ENDOTOXIN OR (1-3)-BETA-D-GLUCAN USING THE SAME - Objects of the present invention are to provide a DNA fragment encoding a | 08-20-2009 |
20090233322 | COLORIMETRIC METHOD AND REAGENT USED FOR THE SAME - The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable. | 09-17-2009 |
20090246816 | METHODS AND KITS FOR SELECTING AN ANALGESIC REGIME - A method is provided for selecting an analgesic regime for treatment of a patient. In the method, a body fluid sample from the patient is assayed for the presence of a metabolite produced by metabolism of a prodrug. If the metabolite is present in the body fluid sample this indicates that a suitable analgesic regime for the patient should comprise administration of at least one analgesic agent selected from the group consisting of: codeine and tramadol. If the metabolite is absent from the body fluid sample indicates that a suitable analgesic regime for the patient should comprise administration of an analgesic agent selected from the group consisting of: dihydrocodeine; buprenorphine and propoxyphene. The invention also provides a kit for selecting an analgesic regime for a patient. The kit comprises means for assaying for the presence of a metabolite produced by metabolism of a prodrug and instructions for using the provided means to perform the method as described above. | 10-01-2009 |
20090246817 | DRY ANALYTICAL ELEMENT CAPABLE OF REDUCING INFLUENCE OF HEMOLYSIS FOR BODY FLUID COMPONENT MEASUREMENT - It is an object of the present invention to reduce influence of hemolysis in a dry analytical element used for measurement of components in a body fluid sample such as blood. The present invention provides a method for producing a dry analytical element for body fluid component measurement comprising at least a reagent layer containing an H | 10-01-2009 |
20090263845 | METHOD AND KIT FOR ASSESSING THE SUITABILITY OF ERYTHROCYTES FOR TRANSFUSION - A method of assessing whether or not erythrocytes are suitable for transfusion into a recipient comprising (i) assaying a sample of erythrocytes for free choline, and (ii) comparing the level of free choline in the sample with the level of free choline deemed suitable for transfusion therapies; and a kit for use in such a method comprising at least one reagent for assaying the sample, instructions for conducting the assaying of the sample, and guidelines for assessing the suitability of the erythrocytes for transfusion. | 10-22-2009 |
20090263846 | Oxygen-resistant hydrogenases and methods for designing and making same - The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H | 10-22-2009 |
20090325209 | METHOD AND REAGENT FOR MEASURING NITROREDUCTASE ENZYME ACTIVITY - Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays. The dyes are of the structure: | 12-31-2009 |
20100021952 | Tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency and its use - A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps of
| 01-28-2010 |
20100041088 | MUTANT PROTEIN HAVING DIAPHORASE ACTIVITY - Mutant diaphorase is capable of having, when forming a complex with a coenzyme, flavin mononucleotide, a conformation in which the distance between tryptophan at the 60th position from an N terminus and imino nitrogen at the 1-position of the coenzyme, flavin mononucleotide, is larger than that in a conformation of wild-type diaphorase of sequence ID No. 1. | 02-18-2010 |
20100047838 | YEAST GENES THAT AFFECT VIRAL REPLICATION - An antiviral agent comprising an altered MAB1, MAB2, MAB3, or OLE1 gene, gene homologs or related genes is disclosed. In another embodiment, the present invention is a method of creating a virus resistant organism comprising creating a transgenic organism comprising an antiviral agent selected from the group of altered MAB1 genes, MAB2 genes, MAB3 genes or OLE1 genes, homologs of these genes, related genes and combinations of these genes and homologs. | 02-25-2010 |
20100062469 | METHOD OF MEASURING 1,5-ANHYDROGLUCITOL IN WHOLE BLOOD, AND SENSOR CHIP AND MEASUREMENT KIT TO BE USED IN THE METHOD - By a method for measuring 1,5-anhydroglucitol, comprising the steps of: eliminating or converting glucose interfering with the measurement of 1,5-anhydroglucitol and/or a derivative thereof beforehand; and measuring 1,5-anhydroglucitol performed thereafter, wherein such glucose and/or a derivative thereof are/is eliminated or converted in whole blood as such without performing blood cell separation, an enzyme for measuring 1,5-anhydroglucitol is allowed to act on without performing blood cell separation, and 1,5-anhydroglucitol is electrochemically measured, it becomes possible to measure 1,5-anhydroglucitol using a small amount of whole blood without resort to a centrifuge or the like. Accordingly, this measurement method can be applied to rapid measurement of 1,5-anhydroglucitol at bedside or in a medical examination room or to home self-measurement thereof by a patient. | 03-11-2010 |
20100062470 | LUMINOGENIC COMPOUNDS AND METHODS TO DETECT CYTOCHROME P450 3A ENZYMES - Described herein are antibacterial compounds, methods for making the compounds, pharmaceutical compositions containing the compounds and methods of treating bacterial infections utilizing the compounds and pharmaceutical compositions. | 03-11-2010 |
20100068746 | DIAGNOSIS METHOD FOR FATTY LIVER DISEASE, DIAGNOSIS APPARATUS, DIAGNOSIS PROGRAM, DIAGNOSTIC AGENT, AND METHOD FOR SCREENING FOR THERAPEUTIC AGENT FOR FATTY LIVER DISEASE - There is provided an examination method which is simpler than conventional liver biopsy and enables the diagnosis of steatohepatitis. This diagnostic method comprises the steps of measuring the concentration of choline contained in a blood-derived sample collected from a subject; and determining the presence of fatty liver disease, severity of the disease, or a therapeutic effect on the disease based on the choline concentration. | 03-18-2010 |
20100093011 | AMINE-CONTAINING COMPOUND ANALYSIS METHODS - The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound. | 04-15-2010 |
20100105095 | Optical Molecular Sensors for Cytochrome P450 Activity - The invention provides a compound, useful as an optical probe or sensor of the activity of at least one cytochrome P450 enzyme, and methods of using the compound to screen candidate drugs, and candidate drugs identified by these methods. The optical probe of the invention is a compound having the generic structure Y-L-Q, wherein Y is selected from the group consisting of Q as herein defined, saturated C | 04-29-2010 |
20100105096 | METHOD OF EVALUATING FOAM-HOLDING PROPERTY OF FERMENTED MALT DRINK AND MARKER FOR EVALUATING FOAM-HOLDING - The present invention provides a method for determination of the foam stability of a fermented malt beverage, wherein the concentration of yeast thioredoxin (M | 04-29-2010 |
20100112622 | METHOD FOR DETECTING PHENOTHIAZINE-DERIVATIVE COLOR AND COLOR-DEVELOPER REAGENT USED THEREIN - The present invention provides a phenothiazine-derivative color-measuring method that can detect a phenothiazine-derivative color even at a wavelength longer than the wavelength that exhibits maximum absorption. A phenothiazine-derivative color is detected, by adding a 5-hydroxy-1-(4-sulfophenyl)-4-(4-sulfophenylazo)pyrazole-3-carboxylic acid salt, 6-hydroxy-5-(4-sulfophenylazo)-2-naphthalenesulfonic acid salt, 3-hydroxy-4-(4-sulfonaphthylazo)-2,7-naphthalenedisulfonic acid salt, 7-hydroxy-8-(4-sulfonaphthylazo)-1,3-naphthalenedisulfonic acid salt, 3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodospiro[isobenzofuran-1(3H),9′-(9H)xanthene]-3-one salt, 3′,6′-dihydroxy-2′,4′,5′,7′-tetrabromo-4,5,6,7,-tetrachlorospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-3-one salt, 4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-3-one salt or flavonoid-based color to the reaction system containing a phenothiazine-derivative color, and then measuring the light absorbance at a wavelength of 610 to 730 nm. | 05-06-2010 |
20100136595 | HYDROCARBON-FORMING OXIDATIVE DECARBONYLASE ENZYME, HYDROCARBONS PRODUCED THEREBY, AND METHOD OF USE - The present disclosure relates to oxidative decarbonylase enzymes, methods of making hydrocarbons with such enzymes, hydrocarbons produced therefrom and uses thereof. More particularly, the present disclosure relates to isolated polypeptide sequences that are cytochrome P450 enzymes with oxidative decarbonylase activity and methods of their use to generate hydrocarbon products, such as biofuels. | 06-03-2010 |
20100167328 | BLOOD CELL SEPARATION - There is provided a method of isolating foetal cells from an isolated sample of maternal blood, the method comprising identifying cells having a different expression pattern of at least one foetal marker compared to the expression pattern of the marker in an equivalent maternal cell and selecting the identified cells, characterised in that the foetal marker is selected from: HSP-60, a monoamine oxidase, glutamine synthase, Ara-70, Ara-54, FLJ20202, DCN-I protein, RAB5A, HSP-7C, EF1A1, GRP78, MYL4, DnaJ homolog subfamily B member 14, Vinculin, Desmoplakin, AMMECR1-like protein, Extracellular matrix protein 2 precursor protein, uncharacterised protein Cxorf57, Peroxiredoxin 1, Peroxiredoxin 2. There is also provided a method of cultivating foetal cells and a foetal cell isolation kit. | 07-01-2010 |
20100173342 | Biomarker and Method for Determining an Oxidative Stress Level - The present invention relates to a biomarker and a method for determining an oxidative stress level in a biological sample, which employs co-factor-dependent oxidative stress parameters, as well as a kit adapted for carrying out such a method. Preferably the co-factor is tetrahydrobiopterin. | 07-08-2010 |
20100190196 | D-SERINE DEHYDRATASE AND USE THEREOF - A novel D-serine quantification method that can overcome various disadvantages of a conventional D-serine quantification method; a novel enzyme that can be used in the D-serine quantification method; a gene encoding the enzyme; and the like. Specifically, a novel D-serine dehydratase including (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 1 and having a D-serine dehydratase activity; and a D-serine quantification method including the steps of reacting a sample with the enzyme, quantifying ammonia or pyruvic acid produced by the reaction, and calculating the amount of D-serine in the sample based on a value produced by the quantification. | 07-29-2010 |
20100196942 | BIOMARKERS OF LIVER INJURY - Novel, sensitive and specific markers for diagnostics and monitoring of liver injuries, including, but not limited to ischemic liver damage, are provided. This includes identification several enzymes of arginine/urea/nitric oxide cycle, sulfuration enzymes and spectrin breakdown related products, among others. | 08-05-2010 |
20100203567 | ESBL Detection Kit and Method - The invention provides methods and detection kits for detecting extended spectrum β-lactamase producers (ESBLs). The kits typically comprise a carrier containing: a)at least one sugar; b) at least one antibiotic for killing Gram positive bacteria; c) at least one antifungal compound; d) a mixture of cefpodoxime and at least one additional antibiotic for killing Gram negative bacteria, and/or a mixture of cefotaxime and ceftazidime; and e) a pH indicator. The methods and kits can be used to by non-specialist health professionals to give rapid results at relatively low cost. | 08-12-2010 |
20100221764 | NOVEL NITROREDUCTASE ENZYMATIC SUBSTRATES - The present invention relates to an enzyme substrate for detecting nitroreductase activity of formula (I) below: | 09-02-2010 |
20100227354 | Autoimmune Conditions and NADPH Oxidase Defects - The invention relates to methods and materials involved in diagnosing and treating autoimmune conditions. In particular, the invention relates to methods and materials involved in diagnosing arthritis conditions that are accompanied by an NADPH oxidase deficiency, methods and materials involved in treating, preventing, or delaying the onset of arthritis conditions that are accompanied by an NADPH oxidase deficiency, and methods and materials involved in identifying agonists and antagonists of NADPH oxidase activity. | 09-09-2010 |
20100233746 | Inflammation and Oxidative Stress Level Assay - The present invention relates to a method for determining the systemic metabolic status of an organism in relation to inflammation and oxidative stress using a biological sample (Inflammation and Oxidative Stress Level Assay). This comprises detection and quantification of one or more derivatives of arachidonic acid (eicosanoids), linoleic acid and/or docosahexaenoic acid, preferably together with one or more oxidative stress parameters and/or with one or more analytes from other metabolite classes in parallel, as well as a kit adapted for carrying out such a method. Moreover, the invention relates to the biomarkers as employed in the method. | 09-16-2010 |
20100240084 | USE OF THE PROTIEN MABA (FABG1) OF MYCOBACTERIUM TUBERCULOSIS FOR DESIGNING AND SCREENING ANTIBIOTICS - The protein MabA, also named protein FabG1, which is recombinant in a purified form, or the recombinant proteins derived from the protein MabA by mutation of at least one amino acid. The uses of proteins MabA, or recombinant proteins derived from protein MabA by mutation of at lease one amino acid, proteins and to the crystalloghraphis co-ordinates thereof, in terms of the implementation of methods for designing and screening ligands of these proteins, and advantageously, ligands inhibiting the enzymatic activity of these proteins. | 09-23-2010 |
20100248282 | DETECTION OF NO BY USING GUANYLYL CYCLASE AND cGMP PRODUCTION AS A READOUT SYSTEM - The present invention relates to a method for determining NO enzymatically and its use for the identification of substances which can modulate a nitric oxide synthase activity. | 09-30-2010 |
20100285514 | ELECTROCHEMICAL BIOSENSOR - Networks of single-walled carbon nanotubes (SWCNTs) decorated with Au-coated Pd (Au/Pd) nanocubes are employed as electrochemical biosensors that exhibit excellent sensitivity (2.6 mA mM | 11-11-2010 |
20100304415 | CHROMATOGRAPHY OF METAL COMPLEXES - A high performance liquid chromatography method to routinely and reproducibly detect and quantitate metal complexes is provided. The metal complexes used in the method of the invention can be different metal complexes, or they can be stereoisomers of the same metal complexes. The high performance liquid chromatography method of the present invention is suitable for the separation of diastereomers of the same metal complexes. Also provided is a chiral high performance liquid chromatography method to separate enantiomers of metal complexes. Superoxide dismutase mimetic compounds are also provided. | 12-02-2010 |
20100304416 | PROSTAGLANDIN E2 MODULATION AND USES THEREOF - Methods, uses, kits and products are described for the prognosis, diagnosis, prevention and treatment of myotronic dystrophy type 1 (DM1), and more particularly for the prognosis, diagnosis, prevention and treatment of the congenital form of myotronic dystrophy type 1 (cDM1), based on changes in/modulation of prostaglandin E | 12-02-2010 |
20100323382 | METHOD FOR ASSAYING BILIRUBIN AND ASSAY INSTRUMENT USED IN BILIRUBIN ASSAY - A method for assaying bilirubin in which a dry reagent is used, which is capable of accelerating the reaction of bilirubin oxidase, and an assay instrument to be used in the method for assaying bilirubin, are provided. The method for assaying bilirubin is characterized in that a biological sample and a bilirubin oxidase-containing dry reagent are mixed first, and then the mixture obtained and a surfactant-containing dry reagent are mixed. An assay instrument ( | 12-23-2010 |
20110014641 | METHOD FOR MEASURING AROMATASE ACTIVITY - The present invention relates to compounds useful for measuring aromatase activity. The invention further provides methods for measuring aromatase activity and for screening test agents which modulate aromatase activity. A kit is also provided for use in such screening methods. | 01-20-2011 |
20110027816 | BIOSENSOR SYSTEM, SENSOR CHIP, AND METHOD OF MEASURING ANALYTE CONCENTRATION IN BLOOD SAMPLE - The present invention provides a biosensor system that can prevent a measurement error caused by the temperature of the environment in use from occurring. A biosensor system | 02-03-2011 |
20110059475 | STABILISATION OF BLOOD CELL CONJUGATES - The invention relates to methods and kits for stabilising blood cell conjugates in blood samples for subsequent analysis. The invention particularly relates to methods and kits for use in fixing and stabilising platelet aggregates in blood samples, and methods for detecting the degree of platelet aggregation in a sample. The invention extends to kits for monitoring the efficacy of anti-thrombotic treatment regimes using platelet aggregation analysis. Two compositions with aliphatic aldehydes and a buffer are employed in a consecutive order. The second composition comprises addition a chelating agent. | 03-10-2011 |
20110081673 | Diagnostic Gum for Screening Diabetes - A diagnostic chewing gum for identifying a risk for diabetes includes a mixture of an enzyme, a conjugated protein of the glucose enzyme, a substrate, and a gum base. The enzyme and the conjugated protein facilitate a conversion of the substrate to produce a detectable signal in the presence of glucose to produce a detectable change in the gum. In use, the gum is chewed, the resulting change in the gum is compared against a chart, and the risk for diabetes is determined from the chart. | 04-07-2011 |
20110104728 | Biliverdin from a Non-Animal Source - Methods for producing biliverdin in a microorganism, methods for producing biliverdin from a non-animal source, cells for producing biliverdin and methods for producing cells for producing biliverdin are disclosed. | 05-05-2011 |
20110151496 | METHODS FOR IDENTIFYING BACTERIAL STRAINS THAT PRODUCE L-TYROSINE - The invention relates to high-throughput screens for identifying bacterial strains capable of L-tyrosine production. | 06-23-2011 |
20110195444 | NOVEL PROTEIN HAVING FRUCTOSYL VALYL HISTIDINE OXIDASE ACTIVITY, MODIFIED PROTEIN, AND USE OF THE PROTEIN OR THE MODIFIED PROTEIN - The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from | 08-11-2011 |
20110229923 | CRYSTAL OF A CYTOCHROME-LIGAND COMPLEX AND METHODS OF USE - The teachings relates to the three-dimensional structure of a crystal of a cytochrome protein complexed with a ligand. The three-dimensional structure of four cytochrome P450 2A6-ligand complexes are disclosed. Cytochrome P450 2A6-ligand crystal structures, wherein the ligand is an inhibitor molecule, are useful for providing structural information that may be integrated into drug screening and drug design processes. Thus, the teachings also relate to methods for utilizing a crystal structure of a cytochrome P450 2A6-ligand complex for identifying, designing, selecting, or testing inhibitors of the cytochrome protein. Such inhibitors are useful as therapeutics for the treatment or modulation of i) diseases; ii) disease symptoms; or iii) the effect of other physiological events mediated by the cytochrome. | 09-22-2011 |
20110256568 | METHODS FOR THE DETECTION AND/OR QUANTIFICATION OF GRAM POSITIVE BACTERIAL CONTAMINANTS - The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial contaminant. | 10-20-2011 |
20110256569 | COMPLEX OF A CHAPERONE WITH BETA-AMYLOID AND METHODS EMPLOYING THIS COMPLEX - A chaperone protein Q2 and β-amyloid can form a complex. This complex can be detected in a biological sample, such as, for example, tissues or fluids from a mammal. Q2 levels can also be detected in a biological sample. A method for determining the Q2 level in a biological sample and comparing that level to a normal Q2 level can be used to detect, screen, diagnose, or otherwise determine a person's susceptibility to Alzheimer's disease such as, for example, the presence or absence of Alzheimer's disease, of symptoms of this disease, of factors leading to or associated with this disease, of likelihood of developing this disease, and the like. In one embodiment, a decline in Q2 level correlates to an increased likelihood of developing Alzheimer's disease. In another embodiment, a decline in Q2 level correlates to an increase in βamyloid aggregation. The method may further include screening for an apolipoprotein E genotype, which is associated with Alzheimer's disease. | 10-20-2011 |
20110262949 | GENETIC INCORPORATION OF 3-AMINOTYROSINE INTO REDUCTASES - This invention provides reductase proteins that comprise NH | 10-27-2011 |
20110269164 | Measuring Device, Measuring Method, and Program - A measuring device ( | 11-03-2011 |
20110287466 | Aggregate-Free Urate Oxidase for Preparation of Non-Immunogenic Polymer Conjugates - A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates. | 11-24-2011 |
20110287467 | METHODS OF PRODUCING RECOMBINANT HEME-BINDING PROTEINS AND USES THEREOF - The present invention is directed to methods of producing recombinant functional heme-binding proteins with complete heme incorporation and purified preparations of the same. The present invention is further directed to methods of identifying agents that modulate the activity of heme-binding proteins. | 11-24-2011 |
20110300567 | METHOD FOR DETERMINING REDOX ACTIVITY AND SCREENING COMPOUNDS BASED ON REDOX ACTIVITY - A method for identifying the redox activity of a subject compound is disclosed. The method can be performed aerobically and can include forming a mixture comprising a free-radical precursor and a compound to be tested, and converting the free-radical precursor into a free-radical anion and a free-radical cation. After the free radical cation and the free radical anion have been formed, the relative redox activity of the subject compound may cause a difference in the rate of photo-bleaching of the mixture and/or the rate of superoxide generation. These differences can be quantified and used to identify the redox activity of the subject compound. This sensitive technique for measuring redox activity can be used to screen compounds for various biological applications. Drugs also can be developed based on the relationship between redox activity and biological activity for particular biological applications. | 12-08-2011 |
20110312011 | DIAGNOSTIC DEVICES AND METHODS OF USE - The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle. | 12-22-2011 |
20120015390 | Methods for the Detection and Treatment of Aberrant Prion Disease - The invention relates to methods and tools for detecting and treating patients suffering from aberrant prion functioning or Aberrant Prion Disease (APD), methods for determining the presence of aberrant prion functioning-inducing agents in a sample and to methods for determining the appropriate therapy for a patient having symptoms of aberrant prion functioning based on detecting aberrant NADH oxidase activity. | 01-19-2012 |
20120100566 | CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES - Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: 0-0 ΛR R R T (i) where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein Ri is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds. | 04-26-2012 |
20120100567 | Measurement Method Using Oxidase - A method for measuring a target object in a sample by using an oxidase, wherein the influence of dissolved oxygen in the sample can be corrected, is provided. The method comprises: obtaining measurement values by causing the target object in the sample to react with the oxidase under different conditions of two or more types; and performing a correction based on the obtained two or more measurement values and a correction method preliminarily set so as to correct the influence of dissolved oxygen in the sample. | 04-26-2012 |
20120122136 | Self-immolative probes for enzyme activity detection - Provided is a compound comprising the structure: | 05-17-2012 |
20120122137 | Novel Nitroreductase Enzymatic Substrates - The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W | 05-17-2012 |
20120129204 | NOVEL NITROREDUCTASE ENZYMATIC SUBSTRATES - The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W | 05-24-2012 |
20120129205 | Novel Nitroreductase Enzymatic Substrates - The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity or as an enzymatic substrate for the detection of a nitroreductase activity and indicator of pH, wherein:
| 05-24-2012 |
20120142040 | Methods and Kits for Quantitative Methyltransferase and Demethylase Measurements - The invention provides methods and kits for characterizing the activity of a methyltransferase or demethylase. The method involves enzymatically methylating or demethylating in vitro a substrate that is a peptide fragment of a full-length polypeptide, and then non-enzymatically methylating the peptide substrate with methyl groups that differ in molecular weight from the enzymatically added or removed methyl groups. Typically, deuterated or | 06-07-2012 |
20120208226 | FRUCTOSYL AMINO ACID OXIDASE - In one form, a mutant fructosyl amino acid oxidase modified at an amino acid residue involved in a proton relay system is provided. The mutant fructosyl amino acid oxidase has reduced oxidase activity while substantially maintaining its dehydrogenase activity. Other forms include an assay device and assay method for measuring glycated protein. Still, other forms include unique methods, techniques, systems and devices involving a mutant fructosyl amino acid oxidase. | 08-16-2012 |
20120288884 | QUINAZOLINONE BASED FLUOROGENIC PROBES - The present invention relates to a compound of the general formula (I) useful in the determining the presence, amount or activity of an enzyme in living cells, a method of preparing said compounds and a kit thereof. | 11-15-2012 |
20120288885 | METHOD AND KIT FOR MEASURING ENZYMATIC ACTIVITIES OF VARIOUS CYTOCHROME P450 MOLECULE SPECIES COMPREHENSIVELY AND WITH HIGH EFFICIENCY - The present invention relates to a method, and a kit, for measuring the enzymatic activity of cytochrome P450 comprehensively and with high efficiency and accuracy, wherein an oxygen sensing layer and a cytochrome P450-supporting layer are vertically integrated on a chip, and cytochrome P450 is supported in a hydrophilic polymer matrix in the cytochrome P450-supporting layer,
| 11-15-2012 |
20120315659 | Reagentless Ceria-Based Colorimetric Sensor - A colorimetric reagent in the form of nanoparticles, composite nanoparticles, and nanoparticle coatings, including methods of use, methods of preparation, deposition, and assembly of related devices and specific applications. The colorimetric reagent comprises cerium oxide nanoparticles which are used in solution or immobilized on a solid support, either alone or in conjunction with oxidase enzymes, to form an active colorimetric component that reacts with an analyte to form a colored complex. The rate of color change and the intensity of the color are proportional to the amount of analyte present in the sample. Also described is the use of ceria and doped ceria nanoparticles as an oxygen storage/delivery vehicle for oxidase enzymes and applications in biocatalytic processes in anaerobic conditions of interest in biomedicine and bioanalysis. Further described are a variety of related applications of the disclosed technology including clinical diagnosis, in vivo implantable devices, food safety, and fermentation control. | 12-13-2012 |
20120322095 | Novel 2,5-Diketo-L-Gluconic Acid Reductases and Methods Of Use - Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described. | 12-20-2012 |
20130052677 | PEG-Urate Oxidase Conjugates and Use Thereof - A naturally occurring or recombinant urate oxidase (uricase) covalently coupled to poly(ethylene glycol) or poly(ethylene oxide) (both referred to as PEG), wherein an average of 2 to 10 strands of PEG are conjugated to each uricase subunit and the PEG has an average molecular weight between about 5 kDa and 100 kDa. The resulting PEG-uricase conjugates are substantially non-immunogenic and retain at least 75% of the uricolytic activity of the unmodified enzyme. | 02-28-2013 |
20130065265 | Biomarker and Method for Determining an Oxidative Stress Level - The present invention relates to a biomarker and a method for determining an oxidative stress level in a biological sample, which employs co-factor-dependent oxidative stress parameters, as well as a kit adapted for carrying out such a method. In one aspect the co-factor is tetrahydrobiopterin. | 03-14-2013 |
20130071868 | MUTANT LACTATE OXIDASE WITH INCREASED STABILITY AND PRODUCT, METHODS AND USES INVOLVING THE SAME - The present disclosure relates to a mutant lactate oxidase having increased stability, a nucleic acid encoding the mutant lactate oxidase, an expression vector comprising the nucleic acid, a host cell comprising the nucleic acid or the expression vector, a method of determining lactate in a sample, the use of the mutant lactate oxidase for determining lactate, a device for determining lactate in a sample using the mutant lactate oxidase and a kit for determining lactate comprising the mutant lactate oxidase. | 03-21-2013 |
20130071869 | Analysis Compensation Including Segmented Signals - A biosensor system determines analyte concentration from an output signal generated from a light-identifiable species or a redox reaction of the analyte. The biosensor system compensates at least 50% of the total error in the output signal with a primary function and may compensate a portion of the residual error with at least one residual function. An SSP function may serve as the primary function, first residual function, or second residual function. Preferably, when the SSP function serves as the first residual function, the SSP function compensates at least 50% of the residual error remaining after primary compensation. Preferably, when the SSP function serves as the second residual function, the SSP function compensates at least 50% of the residual error remaining after primary and first residual compensation. The error compensation provided by the primary, first residual, and second residual functions may be adjusted with function weighing coefficients. | 03-21-2013 |
20130071870 | Method for Determining the Stability of Organic Methyleneamines in the Presence of Semicarbazide-Sensitive Amine Oxidase - The present invention provides methods for determining the stability of methyleneamine, methyleneamine-like compounds or compounds containing a methyleneamine moiety in the presence of semicarbazide-sensitive amine oxidase (SSAO) or a biological sample containing SSAO activity. The disclosed methods may be configured in an assay format for high throughput screening applications. | 03-21-2013 |
20130078662 | Bacillus Pumilus Bilirubin Oxidase and Applications Thereof - The present invention relates to a novel | 03-28-2013 |
20130102018 | Device and Method for Monitoring and Quantifying Analytes - The present invention can be described as an apparatus and cartridge for detecting the presence of an analyte in a fluid. The invention can also be described as a method of detecting the presence of an analyte in a fluid using the apparatus and cartridge. The method comprises the steps of clamping a sensor cartridge into a cartridge receiver of the apparatus, providing fluid to a fluid flow-path of the cartridge, illuminating at least a portion of a sensor, and using a photodetector to detect a change in the optical property of the sensor, wherein the change is caused by the presence or absence of an analyte in the fluid. | 04-25-2013 |
20130137127 | DATING BLOODSTAINS AND BIOLOGICAL FLUIDS WITH FLUORESCENCE LIFETIME TECHNIQUES - Methods of aging biological samples through the use of fluorescence lifetime are disclosed herein. These methods provide aging of samples such as bloodstains using endogenous fluorophores and conformational protein changes. Advantageously, the methods provide the average fluorescence lifetime across a region of interest in a biological sample, thereby minimizing problems with sampling and providing accurate results. | 05-30-2013 |
20130143253 | METHOD FOR QUANTITATIVE MEASUREMENT OF GASTRIC ACIDITY USING 13C CARBONATE SALT - The present invention provides a method for measuring the gastric acidity of a mammal using a | 06-06-2013 |
20130157301 | Diagnosis of bacterial meningitis based on the measure of ROS production in a sample of cerebrospinal fluid - The present invention pertains to a method for in vitro diagnosing a bacterial infection in a biological fluid selected amongst cerebrospinal fluid, ascitic fluid, pericardial fluid, pleural fluid, urine and synovial fluid, based on the measure, in a sample of said fluid, of the production of reactive oxygen species (ROS); a high level of ROS production is indicative of the presence of activated polymorphonuclear neutrophils (PMNs) in said fluid, which in turn is a hallmark of bacterial infection. | 06-20-2013 |
20130189720 | HIGH ENERGY RADIATION INSENSITIVE ANALYTE SENSORS - Enzyme based analyte sensors having radiation stabilizing agents are disclosed and described. More particularly, devices comprising a radiation stabilizing agent and methods for stabilizing sensors to high energy radiation sterilization are disclosed and described. | 07-25-2013 |
20130210052 | A Soluble and Stable Human 5-Lipoxygenase - A soluble and stable form of 5-lipoxygenase (5-LOX) has been made, 5-Lox is the enzyme which initiates leukotriene biosynthesis by catalyzing the two-step transformation of arachidomc acid to leukotriene A4 (LTA4). The soluble and stable 5-LOX is suitable for a number of applications, including, but not limited to, high throughput screening of 5-LOX inhibitors, structural analysis of the enzyme's active site, designing inhibitors based on the three-dimensional structure of the enzyme's active site, and synthesis of LTA4. Using Stable-5-LOX, the crystal structure for 5-LOX has been resolved and the amino acids defining the active site determined. | 08-15-2013 |
20130230878 | METHOD FOR PRODUCING MONOTERPENE AND MONOTERPINOID COMPOUNDS AND USE THEREOF - In various embodiments, the present disclosure provides a method and enzyme for forming various compounds, such as monoterpenes and monoterpenoid compounds. In a specific example, the present disclosure provides a method for producing one or more of (−)-ipsdienol, (−)-ipsenol, ipsenone, and ipsdienone. The present disclosure also provides methods of using compounds formed from the disclosed method and enzyme. | 09-05-2013 |
20130309705 | METHOD FOR DETERMINING THE CONCENTRATION OF HMG-COA REDUCTASE INHIBITORS - The invention, which belongs to the field of enzymology and pharmaceutical chemistry, relates to a method for determining the concentration of a HMG-CoA reductase inhibitor, and to a method for determining the inhibition rate of HMG-CoA reductase. In particular, the method for determining the inhibition rate of HMG-CoA reductase comprises the following steps: 1) establishing the following enzymatic reaction systems for HMG-CoA reductase: a reaction system for a sample to be tested, to which a sample to be tested is added, and a negative control reaction system, to which deionized water in the same volume as a sample to be tested is added; 2) determining the MVAL concentration in a negative control reaction system and in a reaction system for a sample to be tested by HPLC-MS/MS method, respectively; 3) calculating the inhibition rate of HMG-CoA reductase according to the formula: inhibition rate of HMG-CoA reductase=[(MVAL concentration in a negative control reaction system−MVAL concentration in a reaction system for a sample to be tested)/MVAL concentration in a negative control reaction system]×100%. The inhibition rate of HMG-CoA reductase can be accurately determined by the method. | 11-21-2013 |
20130323772 | 3-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed. | 12-05-2013 |
20130344526 | Method of Analyzing L-Tryptophan in Biological Samples, and Kit Used Therein - Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids. | 12-26-2013 |
20140004550 | METHODS FOR ASSAYING ENZYME-MEDIATED OXIDATIVE DEMETHYLATION | 01-02-2014 |
20140051106 | METHOD FOR ASSAYING OGFOD1 ACTIVITY - The present invention relates to assays for monitoring activity of OGFOD1 activity, in particular, to assays for identifying modulators of OGFOD1 activity. The invention also relates to assays to monitor the prolyl hydroxylase activity of OGFOD1 on its substrate, the human ribosomal protein RPS23. The invention also enables the introduction of 3-hydroxyprolyl residues into peptides and proteins. | 02-20-2014 |
20140080167 | Biosensor Calibration Method - A biosensor calibration method configured to measure a concentration of a specific substance contained in sample liquid to be measured, including: a first step configured to acquire a first output value that is output by the biosensor when first calibration liquid is brought into contact with the biosensor; a second step configured to acquire a second output value that is output by the biosensor when second calibration liquid having a different concentration from that of the first calibration liquid is continuously brought into contact with the biosensor after the first step and determine time for replacement of the biosensor on the basis of the first output value and the second output value. | 03-20-2014 |
20140093900 | STEROID PROFILE IN OVARIAN FOLLICULAR FLUID FOR DIAGNOSIS, PROGNOSIS AND DETERMINING STRATEGIES FOR TREATMENT - Concentrations of endogenous steroids in ovarian follicular fluid are used to develop steroid profiles which provide means for the diagnosis and prognosis of endocrine-related conditions and for identifying and developing appropriate treatments for related conditions, including the identification and development of suitable protocols for in vitro fertilization (IVF), treatment and predictive strategies for successful IVF outcomes and selected uses of oocytes for IVF or embryonic stem cell procedures. | 04-03-2014 |
20140106387 | METHOD FOR CONTROLLING NAD(P)/NAD(P)H RATIO BY OXIDOREDUCTASE - Provided is a method capable of effectively treating various diseases associated with energy excess, such as obesity, diabetes, metabolic syndromes, degenerative diseases and mitochondrial dysfunction-related diseases, via elevation of an NAD(P) | 04-17-2014 |
20140120566 | Bilirubin Oxidase from Magnaporthe Oryzae and Applications Thereof - The present invention relates to a novel bilirubin oxidase from | 05-01-2014 |
20140154722 | APPARATUS FOR ANALYZING BIOMATERIAL - There is provided an apparatus for analyzing a biomaterial. The apparatus includes: a first substrate including a plurality of micro-pillars formed to protrude to a predetermined height, the biomaterial being attached to one surface of the micro-pillar; a second substrate including a plurality of micro-wells, the micro-pillars being insertable into the micro-wells when the first substrate-and the second substrate are combined with each other; and at least one spacer disposed between the first substrate and the second substrate when the first substrate and the second substrate are combined with each other. | 06-05-2014 |
20140154723 | RAPID DETECTION OF PETROLEUM IN FOOD - Described herein is a rapid coupled enzymatic assay to detect polycyclic aromatic hydrocarbon (PAH) compounds in samples. The method uses a detection composition that includes: a buffered solution comprising NADH; naphthalene dioxygenase, naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase, and 1,2-dihydroxynaphthalene dioxygenase. The presence of PAH is determined by noting a color change of the detection composition, either visually or spectrophotometrically. | 06-05-2014 |
20140154724 | BIOSENSING SYSTEM WITH EXTENDED LIFETIME VIA COFACTOR RECYCLING - The present disclosure relates to biosensing systems and biosensing elements having increased storage capability and increased functional lifetimes through using compositions and methods for recycling cofactors. | 06-05-2014 |
20140162303 | Method for marking or immobilising a target structure - Method for marking a target structure, comprising the following steps:
| 06-12-2014 |
20140242626 | NITRITE-REDUCTASE (NIRB) AS POTENTIAL ANTI-TUBERCULAR TARGET AND A METHOD TO DETECT THE SEVERITY OF TUBERCULOSIS DISEASE - The present invention discloses functional nitrite reductase as a potential drug target for anti-tubercular drug development. The present invention also relates to the development of an easy method for identification of nitrite in clinical samples as well as its correlation with the severity of the disease. Presence of active as well as dormant/latent stages of | 08-28-2014 |
20140242627 | STABILIZED FORMULATION FOR LUMINESCENT DETECTION OF LUCIFERASE AND NUCLEOSIDE PHOSPHATES - Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample. | 08-28-2014 |
20140242628 | ANALYTICAL METHOD AND DEVICE FOR MALIC ACID - Analytical methods and devices according to the present disclosure may be used to assay the concentration of malic acid, or related species, in a sample, e.g., preparations of fruit, vegetables, juice, and/or wine. Liquid samples are combined with a reaction mixture, incubated, and a parameter characteristic of the resulting incubated mixture is measured. During the incubation, the reaction mixture generally reacts with the liquid sample to transform malic acid or related species that is present in the liquid sample. The measured parameter may be pressure, pH, an amount (e.g., volume, mass) of CO | 08-28-2014 |
20140248645 | GLUCOSE OXIDASE MUTANTS, COMPOSITIONS, DEVICES, KITS AND USES THEREOF - Compositions, devices, kits and methods are disclosed for assaying glucose with a glucose oxidase mutant that has been modified at an amino acid residue involved in the active site. The glucose oxidase mutant has reduced oxidase activity while substantially maintaining its dehydrogenase activity. | 09-04-2014 |
20140273051 | CHEMICAL SENSING APPARATUS HAVING MULTIPLE IMMOBILIZED REAGENTS - An apparatus sensing two or more reactants or analytes in a sample is provided. The apparatus has an one or more light sources emitting energy with two or more detection targets having an immobilized reagent within the target surface. One or more detectors are provided where the two or more detection targets having immobilized reagent thereon are in communication with the sample and the immobilized reagent interacts with the sample. Energy is incident on the targets from the at least one light source such that the energy is changed by the interaction and the change is in turn detected by the at least one detector and associated with a measurement of the level of the reactant or analyte in the sample. A method of making a sensing apparatus and a method of sensing using the sensing apparatus are also disclosed. | 09-18-2014 |
20140273052 | CHEMICAL SENSING APPARATUS HAVING MULTIPLE IMMOBILIZED REAGENTS - A sensor system in a water treatment system has a housing, controller, one or more light sources, one or more sensors and one or more targets having an immobilized reagent thereon. Light source emits light energy into the housing that is incident upon the target with the immobilized reagent and the reagent being in contact with water from the system. The immobilized reagent interacts with a reactant in the water such that the interaction changes the state of the reagent. When energy from the light source is incident on the target with the immobilized reagent the energy shows a change detectable by the sensor such that the changed energy is detectable by and collected at the sensor and data on the energy is communicated to the controller. The data is then correlated as a representation of a desired variable to be measured for the water in the water treatment system. | 09-18-2014 |
20140273053 | CELL CHIPS - Disclosed is a cell chip and methods for the use thereof, wherein the cell chip includes a substrate made of an opaque material and having a plurality of insertion holes formed therein, a filler made of a transparent material and inserted into each of the insertion holes so as to protrude from the substrate, and a biomatrix which is formed on the filler and immobilizes a biomaterial. Also, another substrate having a plurality of wells which store a fluid is further provided thus forming a 3D cell chip. | 09-18-2014 |
20140287448 | METHOD FOR DETERMINING DEGREE OF MODIFIED POTENCY OF A MEDICAMENT - The invention comprises a method for determining degree of modified potency of a medicament. A medicine is a medicament comprising a therapeutic component and a homeopathic, i.e., activated-potentiated, component, wherein the activated-potentiated component has some physical, chemical or biological affect on the therapeutic component and/or the pharmacological efficacy thereof. The therapeutic component is biologically related to the starting substance of the homeopathic component. An analytical measurement of at least one characteristic parameter of the therapeutic form is made prior to its interaction with the activated-potentiated form. The same analytical measurement(s) are made and after interaction between the therapeutic and activated-potentiated forms. This data is used to confirm the presence of any modified potency is caused by the presence of molecular form in the activated-potentiated form. Further, the claimed analytical measurement of at least one characteristic parameter of the therapeutic form prior to its interaction with the activated-potentiated form and again after such interaction serves to quantify the degree of modifying potency associated with the activated-potentiated form in relative dimensionless activity units (release activity). | 09-25-2014 |
20140295478 | BACTERIAL SURROGATE FOR TESTING OF ANTIMALARIALS: thyA KNOCKOUT AND folA KNOCKOUT BACTERIA FOR TESTING OF INHIBITION OF MALARIAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE - The objective of this invention is to create a double thyA folA knockout | 10-02-2014 |
20140349327 | METHOD FOR PRODUCING FRUCTOSYL VALYL HISTIDINE OXIDASE PREPARATION | 11-27-2014 |
20140356889 | COMPOSITION FROM LOBSTER HEMOCYTE EXTRACTS FOR DETECTION OF LIPOPOLYSACCHARIDES, PEPTIDOGLYCANS AND 1,3-BETA-D-GLUCANS - The present invention relates to the pharmaceutics, biotechnological and chemical, and particularly to a process for preparing a composition for detecting and measuring the concentration of endotoxins or lipopolysaccharides, peptidoglycans and (1,3)-β-D-glucans, using an extract from the hemocytes of the lobster as a starting material, the changes to the composition to increase the sensitivity, and processes for measuring endotoxins, peptidoglycans and (1,3)-β-D-glucans using said composition. | 12-04-2014 |
20140363836 | Method for Determining the Stability of Organic Methyleneamines in the Presence of Semicarbazide-Sensitive Amine Oxidase - The present invention provides methods for determining the stability of methyleneamine, methyleneamine-like compounds or compounds containing an methyleneamine moiety in the presence of semicarbazide-sensitive amine oxidase (SSAO) or a biological sample containing SSAO activity. The disclosed methods may be configured in an assay format for high throughput screening applications. | 12-11-2014 |
20140377791 | NOVEL TWO-PHOTON ABSORBING FLUORESCENT SUBSTANCE, AND SUBSTRATE SENSING METHOD USING SAME - The present invention relates to a compound which is a novel two-photon absorbing fluorescent substance, a production method for the compound, a fluorescence sensor and molecular probe able to sense various substrates or enzyme activity or the like using the same, and a method of sensing enzyme activity or the like using the same. More specifically, the present invention relates to a novel two-photon absorbing fluorescent substance which has the high photo-stability and large two-photon absorption cross-section value of acedan which is a two-photon absorbing fluorescent substance, and has the high fluorescence efficiency of coumarin which is a one-photon absorbing fluorescent substance, while exhibiting absorption and emission characteristics at a longer wavelength than existing acedan and coumarin and so being advantageous in in-vivo imaging. The compound according to the present invention can be used as a fluorescence sensor which is highly sensitive and selective for various substrates, and more particularly, can be used in the study and treatment of diseases in which MAO enzymes are involved such as mood disorders using MAO enzyme activity and inhibitor screening. | 12-25-2014 |
20150050682 | DIRECT ASSAY OF THIOREDOXIN REDUCTASE ACTIVITY - Provided is a direct method for detecting thioredoxin reductase (TR) activity in test samples. The method can provide a continuous and real-time measurement of TR activity. The method comprises contacting the test sample with NADPH and a diselenide substrate of TR, and then measuring conversion of NADPH to NADP. Also provided are kits for use in the method of direct detection of TR activity. | 02-19-2015 |
20150064736 | NOVEL 2,5-DIKETO-L-GLUCONIC ACID REDUCTASES AND METHODS OF USE - Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described. | 03-05-2015 |
20150079619 | AQUEOUS METHACETIN SOLUTIONS LABELLED WITH 13C HAVING PROPYLENE GLYCOL AS A SOLUBILIZER - An analysis method determines a functional parameter of an organ by measuring | 03-19-2015 |
20150118702 | NITRITE-REDUCTASE (NIRB) AS POTENTIAL ANTI-TUBERCULAR TARGET AND A METHOD TO DETECT THE SEVERITY OF TUBERCULOSIS DISEASE - The present invention discloses functional nitrite reductase as a potential drug target for anti-tubercular drug development. The present invention also relates to the development of an easy method for identification of nitrite in clinical samples as well as its correlation with the severity of the disease. Presence of active as well as dormant/latent stages of | 04-30-2015 |
20150125889 | IN VITRO DETECTION OF MICROORGANISMS WITH AZOREDUCTASE ACTIVITY - The present invention relates to the use of at least one azo compound for detecting at least one microorganism in a sample. More precisely, the present invention relates to a process for detecting, in a biological sample, at least one microorganism with azoreductase activity, including the steps: placing the sample in contact with a reaction medium including at least one azo compound, incubating the reaction medium, and detecting the reduction of the azo compound by the microorganism, indicating the presence of the at least one microorganism. | 05-07-2015 |
20150140591 | CELL ENGINEERING USING RNAs - The invention concerns the field of cell culture technology. It concerns RNA having a specific sequence, expression vectors encoding said RNA, production host cell lines comprising said RNA, and methods of producing recombinant biopharmaceutical products using engineered host cell with altered levels of said RNAs, such as small non-coding RNAs, preferably microRNAs (miRNAs). The invention also relates to engineered host cells with altered levels in one or more of said RNAs. Those cell lines have improved secretion and/or growth characteristics in comparison to control cell lines. | 05-21-2015 |
20150140592 | DEVELOPMENT OF MICROORGANISMS FOR HYDROGEN PRODUCTION - Methods and compositions are provided for engineering microorganisms, which permit enhanced H | 05-21-2015 |
20150337333 | REGULATORY NUCLEIC ACID ELEMENTS - The invention relates to DNA-sequences, especially transcription- or expression-enhancing elements (TE elements) and their use on an expression vector in conjunction with an enhancer, a promoter, a product gene and a selectable marker. | 11-26-2015 |
20160002610 | Modified Glycine Oxidase - The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like. | 01-07-2016 |
20160024552 | BIOSENSING SYSTEM WITH EXTENDED LIFETIME VIA COFACTOR RECYCLING - The present disclosure relates to biosensing systems and biosensing elements having increased storage capability and increased functional lifetimes through using compositions and methods for recycling cofactors. | 01-28-2016 |
20160041151 | COMPOSITIONS AND METHODS FOR MONITORING OXALATE - The present invention features compositions and methods for the detection or measurement of oxalate in a sample. Such compositions include test devices that provide for the rapid and accurate detection of oxalate in a sample from a biological fluid. Advantageously, the compositions can be used to monitor the oxalate levels of a patient at a point of care (e.g., at the patient's home, clinic, physician's office, or other clinical setting). | 02-11-2016 |
20160054230 | ENZYMATIC METHOD FOR DETECTING POLYAROMATIC HYDROCARBONS - Polyaromatic hydrocarbons (PAH) from industrial waste and oil spills are a recurrent threat to food safety due to their stability and toxicity. Existing methods to establish the concentrations of PAH in environmental and food samples rely on expensive and laborious methodology. The teachings herein provide methods and kits for detecting the presence or absence of PAH. In certain embodiments, the quantity of PAH in a sample can be determined. | 02-25-2016 |
20160067710 | DEVICE FOR ANALYSIS OF CELLULAR MOTILITY - A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells. | 03-10-2016 |
20160068824 | NOVEL GLUCOSE OXIDASE VARIANTS - The technology provided herein relates to novel variants of microbial glucose oxidase with improved properties, more specifically to polypeptides having glucose oxidase activity as their major enzymatic activity; to nucleic acid molecules encoding said glucose oxidases; vectors and host cells containing the nucleic acids and methods for producing the glucose oxidase; compositions comprising said glucose oxidase; methods for the preparation and production of such enzymes; and to methods for using such enzymes for food and feed processing, for the measurement of free glucose in clinical samples and bioreactors, and the development of miniature biofuel cells. | 03-10-2016 |
20160077069 | GAS SENSOR AND MEMBER USING METAL OXIDE SEMICONDUCTOR NANOFIBERS INCLUDING NANOPARTICLE CATALYST FUNCTIONALIZED BY BIFUNCTIONAL NANO-CATALYST INCLUDED WITHIN APOFERRITIN, AND MANUFACTURING METHOD THEREOF - The inventive concepts relate to a member for a gas sensor, a gas sensor using the same and a manufacturing method thereof, and more particularly, to a member for a gas sensor using a one-dimensional metal oxide nanofiber complex material containing hetero nanoparticle catalysts synthesized using apo-ferritins, a gas sensor using the same, and a manufacturing method thereof. | 03-17-2016 |
20160108451 | BLOOD COMPONENT MEASUREMENT DEVICE AND BLOOD COMPONENT MEASUREMENT METHOD - Provided are a blood component measurement device and the like capable of further suppressing the errors in measuring blood components. A first current value that is generated by oxidation-reduction when a first voltage is applied to a first electrode pair | 04-21-2016 |
20160138075 | DETERMINING USABILITY OF ANALYTICAL TEST STRIP - A system for determining usability of an analytical test strip includes a sample chamber to receive a fluid sample, a reagent in the sample chamber having a moisture-varying impedance, and two detection electrodes contacting the reagent. A test meter applies an AC waveform across the reagent via the detection electrodes while measuring an impedance of the reagent. A processor automatically determines whether the measured impedance of the reagent meets a dryness criterion. The meter includes a housing, a strip port connector, an impedance measurement circuit and the processor. A method for determining usability of a strip inserted in a hand held meter includes applying an AC waveform across a reagent of the strip and measuring a first electrical signal, and determining whether the strip meets the dryness criterion based on the first electrical signal. The test strip and ways of determining an analyte are also described. | 05-19-2016 |
20160186232 | MODIFIED AMADORIASE AND METHOD FOR PRODUCING THE SAME, AGENT FOR IMPROVING SURFACTANT RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING THE SAME - Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus | 06-30-2016 |
20160187339 | Biomarker and Method for Determining an Oxidative Stress Level - The present invention relates to a biomarker and a method for determining an oxidative stress level in a biological sample, which employs co-factor-dependent oxidative stress parameters, as well as a kit adapted for carrying out such a method. In one aspect the co-factor is tetrahydrobiopterin. | 06-30-2016 |
20160194598 | METHOD FOR OPTIMISING THE PRODUCTION EFFICIENCY, ORGANOLEPTIC QUALITY AND STABILITY OVER TIME OF A PROTEIN-RICH MICROALGAE BIOMASS | 07-07-2016 |
20160201109 | Rapid Peptidoglycan-Based Assay for Detection of Bacterial Contamination | 07-14-2016 |