Entries |
Document | Title | Date |
20080213847 | PROCESS FOR PRODUCING PRENYL ALCOHOLS - A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture. | 09-04-2008 |
20080220488 | Process for making isooctenes from dry 1-butanol - The present invention relates to a process for making isooctenes using dry 1-butanol derived from fermentation broth. The isooctenes so produced are useful for the production of fuel additives. | 09-11-2008 |
20080220489 | Efficient use of biogas carbon dioxide in liquid fuel synthesis - The invention provides methods for producing methanol, higher alcohols (containing 2 or more carbons), in particular ethanol, or diesel fuel from biological wastes such as manure, sewage, and crop wastes, as well as from other organic materials. In the methods, organic material is first fermented by anaerobic microorganisms to a biogas consisting primarily of methane and carbon dioxide. The biogas is then converted to synthesis gas consisting primarily of CO and H | 09-11-2008 |
20080274523 | PRODUCTION OF ISOPRENOIDS - The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells. | 11-06-2008 |
20080305534 | Novel Glycerol Dehydrogenase, Gene Therefor, and Method of Utilizing the Same - The present invention provides a polypeptide having physicochemical characteristics of (1) to (5) described below:
| 12-11-2008 |
20090061493 | Lipid Pathway Modification in Oil-Bearing Microorganisms - The invention provides methods of modifying the lipids produced by microbial organisms through genetic engineering. The invention also provides genetically engineered microbes and methods of fermenting microbes for oil production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured by such modified microorganisms. Exemplary oil-bearing organisms include organisms containing one or more exogenous genes encoding a fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein. | 03-05-2009 |
20090081743 | TRANSPORTABLE ALGAE BIODIESEL SYSTEM - A portable system and method for producing biofuel from algae are disclosed. In the portable system, a chemostat and a plug flow reactor formed from plastic bladders are interconnected. Further, an algae separator is in fluid communication with the plug flow reactor for removing algae cells. Also, the system includes a device for processing biofuel from the algae cells. Importantly, the system includes a temperature controller to maintain desired temperatures in the chemostat and plug flow reactor for algae growth and intracellular algae production. In order to further support algae cell growth, the system includes a device for capturing carbon dioxide and delivering the carbon dioxide to the chemostat. | 03-26-2009 |
20090081744 | Method of processing slops - A method of processing slops including production of biogas characterized in that slops are exposed to anaerobic fermentation in a anaerobic fermenter under generation of biogas and at least a portion of the biogas containing methane and carbon dioxide is fed into a first photo-bioreactor, where the carbon dioxide or a part thereof is consumed during a photosynthesis process producing micro-algae and methane and the residual carbon dioxide are hen withdrawn for further processing. | 03-26-2009 |
20090098623 | PROCESS FOR THE RACEMIZATION OF OPTICALLY ACTIVE SECONDARY ALCOHOLS WITH THE USE OF ALCOHOL DEHYDROGENASE - A method for racemizing optically active secondary alcohols by incubating these alcohols with at least one alcohol dehydrogenase of the E.C. 1.1.1. class. | 04-16-2009 |
20090137014 | PRODUCTION OF ISOPRENOIDS - Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells. | 05-28-2009 |
20090137015 | METHOD AND APPARATUS FOR PREPARING MATERIAL FOR MICROBIOLOGICAL FERMENTATIONS - The present invention relates to a method for preparing organic material for microbiological fermentations of municipal waste water sludge for reducing the waste water sludge and/or of cellulosic material for the production of biofuels, with at least one of the following steps: disintegrating and/or liquefying the organic and/or cellulosic material by using a jet pump and/or creating a supersonic shock zone in a jet pump the organic and/or cellulosic material is supplied to in order to disintegrate and/or liquefy the organic and/or cellulosic material and/or applying high pressure steam to an annular ring chamber enclosing a tube of a jet pump and directing the steam into the tube of the jet pump thereby sucking organic and/or cellulosic material into the tube and creating a supersonic shock zone in the jet pump in order to disintegrate and/or liquefy the organic and/or cellulosic material. The present invention also relates to an apparatus for preparing organic material for microbiological fermentations of municipal waste water sludge for reducing the waste water sludge and/or of cellulosic material for production of biofuels comprising a jet pump. | 05-28-2009 |
20090203096 | Process for Production of Optically Active Alcohol - The present invention provides methods for producing (S)-1,1,1-trifluoro-2-propanol, which include the step of reacting an enzyme of any one of alcohol dehydrogenase CpSADH, alcohol dehydrogenase ReSADH, carbonyl reductase ScoPAR, (2S,3S)-butanediol dehydrogenase ZraSBDH, carbonyl reductase ScGCY1, tropinone reductase HnTR1, tropinone reductase DsTR1, or alcohol dehydrogenase BstADHT, a microorganism or a transformant strain that functionally expresses the enzyme, or a processed material thereof, with 1,1,1-trifluoroacetone. The present invention also provides methods for producing (R)-1,1,1-trifluoro-2-propanol, which include the step of reacting alcohol dehydrogenase PfODH, a microorganism or a transformant strain that functionally expresses the enzyme, or a processed material thereof, with 1,1,1-trifluoroacetone. | 08-13-2009 |
20090246842 | ENGINEERED MICROORGANISMS FOR PRODUCING PROPANOL - Methods and compositions for the production of bio-based material precursors are provided. | 10-01-2009 |
20090280545 | MOLECULE PRODUCTION BY PHOTOSYNTHETIC ORGANISMS - The present invention provides compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of products. The methods and compositions are particularly useful in the petrochemical industry. | 11-12-2009 |
20090311763 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 12-17-2009 |
20090325251 | Methods and Systems for Biomass Conversion to Carboxylic Acids and Alcohols - The disclosure includes a method, process and apparatus for the conversion of biomass to carboxylic acids and/or primary alcohols. The system may include a pretreatment/fermentation subsystem operable to produce a fermentation broth containing carboxylic acid salts from biomass, such as lignocellulosic biomass. The system may also include a dewatering subsystem operable to remove excess water from the fermentation broth to produce a concentrated product. The system may also includes an acid springing subsystem operable to produce a mixed carboxylic acid product. The system may also include a hydrogenation subsystem operable to produce an alcohol mixture, such as a mixture containing primary alcohols. Methods of operating this system or other systems to obtain a carboxylic acid or alcohol mixture are also provided. | 12-31-2009 |
20100055751 | KETOREDUCTASE POLYPEPTIDES AND USES THEREOF - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of stereo specifically reducing (R)-2-methylpentanal to (R)-2-methylpentanol. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to produce (R)-2-methylpentanol and related compounds. | 03-04-2010 |
20100062504 | Nerolidol, Terpene, and Terpene Deriviative Synthesis - According to one embodiment, the description relates to a method of nerolidol production. The method includes culturing a yeast strain lacking functional squalene synthase and overproducing HMG CoA reductase in synthetic medium lacking uracil and producing nerolidol. The pH of the medium may be adjusted to an acidic level to further increase nerolidol production. Other chemicals may also be produced by this method. The nerolidol or other chemicals may be removed from the yeast or medium or both. The medium may additionally contain a polyaromatic resin, which may adsorb nerolidol or other chemicals. | 03-11-2010 |
20100112653 | Process of Producing a Fermentation Product - The present invention relates to processes for producing a fermentation product from starch-containing material comprising: (a) saccharifying starch-containing material with an alpha-amylase at a temperature below the initial gelatinization temperature of said starch-containing material; (b) fermenting using a fermenting organism, wherein a phytase is present during steps (a) and/or (b). | 05-06-2010 |
20100120105 | CARBON PATHWAY OPTIMIZED PRODUCTION HOSTS FOR THE PRODUCTION OF ISOBUTANOL - A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudoroff pathway. | 05-13-2010 |
20100203604 | TRANSFORMANT CAPABLE OF PRODUCING ISOPROPANOL - A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into an aerobic bacterium or a facultative anaerobic bacterium:
| 08-12-2010 |
20100221800 | MICROORGANISM ENGINEERED TO PRODUCE ISOPROPANOL - Provided herein are metabolically-modified microorganisms useful for producing biofuels. | 09-02-2010 |
20100233769 | Biomass treatment process - A process for the treatment of biomass is provided. The process comprises forming a biomass slurry by mixing biomass with a working fluid, and inducing the biomass slurry to flow through an inlet into a passage. A high velocity transport fluid is injected into the slurry through a nozzle communicating with the passage. The injection of the high velocity transport fluid applies a shear force to the slurry such that the working fluid is atomised and forms a vapour and droplet flow regime, an at least partial vacuum is formed within the passage downstream of the nozzle, and a condensation shock wave is generated within the passage downstream of the nozzle and vacuum by condensation of the transport fluid. An apparatus for treating biomass using the aforementioned process is also provided. | 09-16-2010 |
20100311135 | ISOPROPYL ALCOHOL- PRODUCING BACTERIUM AND METHOD OF PRODUCING ISOPROPYL ALCOHOL USING THE SAME - The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor. | 12-09-2010 |
20100311136 | RECOMBINANT YEAST AND BRANCHED ALCOHOL PRODUCTION METHOD USING RECOMBINANT YEAST - This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. | 12-09-2010 |
20100311137 | Microorganisms Having Enhanced Tolerance To Inhibitors and Stress - The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention. | 12-09-2010 |
20100323417 | CARBON CAPTURE IN FERMENTATION - The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas. | 12-23-2010 |
20110045560 | METHOD FOR PRODUCING OPTICALLY ACTIVE 2-METHYL-ALKANOLS - A process for preparing optically active 2-methylalkan-1-ol of the general formula (III) comprising the following steps:
| 02-24-2011 |
20110070617 | Liquid methanol fuel production from methane gas at bio-normal temperatures and presure - Through staged and monitored control of gas, liquid, and solid source materials, the highly-efficient enzymatic ‘natural factory’ of specific methanotropic bacteria, which evolved dual, alternative, metabolic channels, can be manipulated for human goals. The first stage sets these bacteria to producing liquid methanol by oxidation of methane gas under aerobic conditions (their high-energy channel), which is harvested at the peak. The second stage, by establishing anaerobic conditions and providing supplementary metals, forces the bacteria to use their lower-energy channel for inorganic hydrogen-donor to organic-energy-transport, during which the older and weaker organisms become ‘food’ for newer and (relatively) stronger organisms. This accomplishes the desired result of liquid methanol production without employing a human-engineered industrial-chemical process with the costly high energy requirements associated with temperatures and pressures required by the prior art for converting methane gas to liquid methanol. | 03-24-2011 |
20110124068 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 05-26-2011 |
20110165642 | TRANSFORMANT OF CORYNEFORM BACTERIA CAPABLE OF PRODUCING ISOPROPANOL - A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into a coryneform bacterium:
| 07-07-2011 |
20110165643 | Separation of Lignin From Hydrolyzate - A method for the production water insoluble reactive lignin having low sulfur content and lignosulfonates from lignocellulosic material in a batch or continuous process. Lignocellulosic material is fractionated to produce water insoluble native lignin and lignosulfonates in various ratios, while preserving the cellulose and hydrolyzed hemicelluloses using water, ethanol and sulfur dioxide. | 07-07-2011 |
20110171705 | PROCESS FOR THE PRODUCTION OF ALCOHOL - The invention provides a process for the production of an alcohol which comprises cleaving polysaccharides in a cellulosic material in an aqueous medium at a temperature of at least 45° C. using a thermophilic microorganism to yield fermentable sugars, fermenting an aqueous solution of said sugars at a temperature of at least 45° C. with a thermophilic microorganism to yield an alcohol or an alkanoate, if necessary reducing said alkanoate to yield an alcohol, and removing said alcohol from said aqueous solution. | 07-14-2011 |
20110195470 | Production of Isoprenoids - The invention provides a biological method of producing isoprenoids. | 08-11-2011 |
20110244537 | REGIO- AND ENANTIOSELECTIVE ALKANE HYDROXYLATION WITH MODIFIED CYTOCHROME P450 - Cytochrome P450 BM-3 from | 10-06-2011 |
20110250661 | Methods for gasification of carbonaceous materials - The present disclosure is generally directed to process of gasification of carbonaceous materials to produce synthesis gas or syngas. The present disclosure provides improved methods of gasification comprising: adding one or more carbonaceous materials, adding a molecular oxygen-containing gas, adding a methane-containing gas and optionally adding water or steam into said gasifier. This disclosure is also directed to process of production of one or more alcohols from said syngas via fermentation or digestion in the presence of at least one microorganism. | 10-13-2011 |
20110250662 | Methods for gasification of carbonaceous materials - The present disclosure is generally directed to process of gasification of carbonaceous materials to produce synthesis gas or syngas. The present disclosure provides improved methods of gasification comprising: adding one or more carbonaceous materials, adding a molecular oxygen-containing gas, adding carbon dioxide gas and optionally adding water into said gasifier. This disclosure is also directed to process of production of one or more alcohols from said syngas via fermentation or digestion in the presence of at least one microorganism. | 10-13-2011 |
20110250663 | METHODS AND COMPOSITIONS RELATED TO FATTY ALCOHOL BIOSYNTHETIC ENZYMES - Compositions and methods for producing fatty acid derivatives using recombinant microorganisms are described herein. | 10-13-2011 |
20110256599 | ENHANCED PRODUCTION OF FATTY ACID DERIVATIVES - Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use. | 10-20-2011 |
20120009640 | FATTY ALCOHOL FORMING ACYL REDUCTASES (FARS) AND METHODS OF USE THEREOF - The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same. | 01-12-2012 |
20120040426 | PRIMARY ALCOHOL PRODUCING ORGANISMS - The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms. | 02-16-2012 |
20120135485 | SORBENT AND PROCESS FOR REMOVING FERMENTATION INHIBITORS - The invention pertains to, for example, an improved sorbent and process for removing fermentation inhibitors such as furfural and/or HMF in microbial processes utilizing fermentable sugars obtained from biomass including, for example, in the production of bioalcohols. The sorbent is capable of separating one or more inhibitors from monosaccharides and is characterized by: (1) a K | 05-31-2012 |
20120135486 | Methods and Compositions for the Extracellular Transport of Biosynthetic Hydrocarbons and Other Molecules - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated. | 05-31-2012 |
20120156745 | METHYL BUTENOL SYNTHASE - The present invention provides novels genes encoding methyl butenol (MBO) synthase, methy butenol synthases and their use in methyl butenol production. | 06-21-2012 |
20120202260 | HYBRID BIOREFINING AND GASIFICATION OF LIGNOCELLULOSIC FEEDSTOCKS - Processes and systems for concurrent recovery of lignin derivatives and syngas from a lignocellulosic feedstock. The processes and systems therefor generally comprise the steps of: (a) perfusing and cooking the lignocellulosic feedstock with a suitable organic solvent for a suitable period of time thereby producing a cellulosic solids output stream and a spent liquid solvent stream, said spent liquid solvent stream comprising solubilized lignin derivatives and other organic compounds; (b) separating said cellulosic solids output stream and said spent liquid solvent stream; (c) recovering lignin derivatives from the spent liquid solvent stream thereby producing at least a partially de-lignified spent liquid solvent stream; (d) recovering a portion of the organic solvent from the at least partially de-lignified spent liquid solvent stream thereby producing a stillage; and (e) gasifying the cellulosic solids output stream thereby producing a combustible syngas. | 08-09-2012 |
20120225462 | NUTRIENT COMPOSITION FOR SACCHAROMYCES CEREVISIAE AND METHOD FOR USING THE SAME - Nutrient composition for | 09-06-2012 |
20120237992 | ISOPROPYL ALCOHOL-PRODUCING BACTERIUM AND METHOD FOR PRODUCING ISOPROPYL ALCOHOL - The present invention provides an isopropyl alcohol-producing | 09-20-2012 |
20120244589 | Photoalkanogens with Increased Productivity - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules. | 09-27-2012 |
20120264184 | ALTERNATIVE PATHS TO ALCOHOLS AND HYDROCARBONS FROM BIOMASS - A method of producing alcohols, hydrocarbons, or both from biomass, the method including converting biomass into a carboxylic acid; reacting the carboxylic acid with an olefin to produce an ester; and hydrogenolyzing the ester to produce alcohol. The steps of reacting the carboxylic acid with an olefin to produce an ester, and hydrogenolyzing the ester to produce an alcohol, may both be carried out in the same reactor. | 10-18-2012 |
20120288909 | MICROBIAL PRODUCTION OF PENTANOL FROM GLUCOSE OR GLYCEROL - The invention relates to the production of pentanol through recombinant gene expression and metabolic engineering. | 11-15-2012 |
20120322117 | ENZYMATIC PRODUCTION OF ALCOHOL ESTERS FOR RECOVERY OF DIOLS PRODUCED BY FERMENTATION - Diols produced in fermentation are processed in broth by esterification of the product diol with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product diol, such as 1,3-propanediol, with the carboxylic acid to form the diol esters. The diol esters can be extracted from the broth, and the product diol recovered from the diol esters. The carboxylic acid can also serve as an extractant for removal of the diol esters from the fermentation medium. | 12-20-2012 |
20120329112 | Microorganisms Having Enhanced Tolerance To Inhibitors and Stress - The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention. | 12-27-2012 |
20130005007 | Method for Industrially Producing(s)-1,1,1-Trifluoro-2-Propanol - Disclosed is a method for producing (S)-1,1,1-trifluoro-2-propanol with high optical purity and high yield by having at least one kind of microorganism, which is selected from the group consisting of | 01-03-2013 |
20130005008 | HIGHLY PRODUCTIVE ISOPROPYL ALCOHOL-PRODUCING BACTERIUM - An isopropyl alcohol-producing | 01-03-2013 |
20130011891 | MICROORGANISMS FOR PRODUCING BUTADIENE AND METHODS RELATED THERETO - The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol. | 01-10-2013 |
20130011892 | RECOMBINANT YEAST AND BRANCHED ALCOHOL PRODUCTION METHOD USING RECOMBINANT YEAST - This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided. | 01-10-2013 |
20130040352 | PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) - The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as | 02-14-2013 |
20130052706 | PROCESS FOR THE PRODUCTION OF ISOPRENOL FROM MEVALONATE EMPLOYING A DIPHOSPHO-MEVOLONATE DECARBOXYLASE - Described is a method for the enzymatic production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol as well as the use of an enzyme which is capable of catalyzing the decarboxylation of mevalonate for the production of isoprenol from mevalonate. Furthermore described is the use of mevalonate as a starting material for the production of isoprenol in an enzymatically catalysed reaction. Also disclosed is a method for the production of isoprene comprising the method for the production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol and further comprising the step of converting the produced isoprenol into isoprene as well as a method for the production of isoamyl alcohol comprising the method for the production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol and further comprising the step of converting the produced isoprenol into isoamyl alcohol. | 02-28-2013 |
20130071900 | PROCESS FOR PROCESSING A LIGNOCELLULOSIC MATERIAL - Calcium-containing lignocellulosic material is converted at a temperature in the range from equal to or more than 120° C. to equal to or less than 210° C. with an aqueous acid solution containing one or more inorganic acids and having a pH in the range from equal to or more than 1.8 to equal to or less than 4.0 to produce a mixture, containing pretreated lignocellulosic material and aqueous acid solution, having an overall pH in the range from equal to or more than 3.0 to equal to or less than 4.5. The process allows reduction in the formation of insoluble salts. | 03-21-2013 |
20130095542 | ENGINEERED MICROORGANISMS AND INTEGRATED PROCESS FOR PRODUCING N-PROPANOL, PROPYLENE AND POLYPROPYLENE - The invention provides fermentative methods for producing n-propanol. The methods of the invention involve providing a suitable carbon source, a microorganism expressing the dicarboxylic acid pathway, reducing equivalents, and at least one gene coding for an enzyme that catalyzes the conversion of propionate/propionyl-CoA into n-propanol. The methods further involve contacting the carbon source and reducing equivalents with the microorganism under conditions favorable for the production of n-propanol. Also provided are methods for producing propylene and polypropylene from the n-propanol and microorganisms suitable for use in the methods of the invention. | 04-18-2013 |
20130164805 | Recombinant Microorganisms Having Enhanced Propanol and Method for Preparing Propanol Using the Same - The present invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, and to a method of producing propanol using the same. More particularly, the invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, which have introduced therein genes that encodes enzymes which are involved in the biosynthesis of propanol from threonine, and to a method of producing propanol using the same | 06-27-2013 |
20130177954 | METHOD FOR PRODUCING ISOPROPANOL AND RECOMBINANT YEAST CAPABLE OF PRODUCING ISOPROPANOL - Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced. | 07-11-2013 |
20130203140 | PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS - Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process. | 08-08-2013 |
20130252295 | Production of Isoprenoids - Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control of at least one heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells. | 09-26-2013 |
20130273625 | METHODS FOR INCREASING MICROBIAL PRODUCTION OF ISOPRENE, ISOPRENOIDS, AND ISOPRENOID PRECURSOR MOLECULES USING GLUCOSE AND ACETATE CO-METABOLISM - Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels. | 10-17-2013 |
20130273626 | REGIO- AND ENANTIOSELECTIVE ALKANE HYDROXYLATION WITH MODIFIED CYTOCHROME P450 - Cytochrome P450 BM-3 from | 10-17-2013 |
20130280775 | Recombinant N-propanol and Isopropanol Production - The present invention relates to methods of producing n-propanol, isopropanol, and coproducing n-propanol with isopropanol. The present invention also relates to methods for producing propylene, as well as host cells capable of n-propanol and isopropanol production. | 10-24-2013 |
20130295627 | PROCESS FOR PRODUCING METHYL BUTENOL (2-METHYL-3-BUTEN-2-OL) - Disclosed is a process for producing 2-methyl-3-buten-2-ol (MBO). The process is carried out by fermenting a hydrocarbon in a fermentation medium in the presence of a MBO-producing microorganism to produce the MBO. The MBO is removed from the fermentation medium to maintain a concentration of MBO non-toxic to the MBO-producing microorganism by flowing a gas through or across the fermentation medium to effectively remove at least a portion of the MBO into a vapor phase region of the fermentation medium. The rate of removal is measured according to the amount of MBO maintained in the vapor phase region, which is measured according to the vapor pressure of the MBO in the vapor phase region. | 11-07-2013 |
20130316415 | Production of Branched-Chain Alcohols by Photosynthetic Microorganisms - The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses. | 11-28-2013 |
20140093928 | Methods and Systems for Biologically Producing Methanol - A method for biologically producing methanol is disclosed. In some embodiments, the method includes the following: providing a biomass including ammonia oxidizing bacteria having ammonia monooxygenase enzymes and hydroxylamine oxidoreductase enzymes; feeding ammonia to the ammonia oxidizing bacteria; feeding a non-substrate-organic compound including methane to the ammonia oxidizing bacteria; feeding oxygen and reducing equivalents (in the form of hydroxylamine) to the ammonia oxidizing bacteria; oxidizing the ammonia using the ammonia monooxygenase enzymes in the ammonia oxidizing bacteria to generate hydroxylamine and oxidizing the hydroxylamine using the hydroxylamine oxidoreductase enzymes to form nitrite; and partially oxidizing the methane in the compound using the ammonia monooxygenase enzymes in the ammonia oxidizing bacteria to generate methanol. | 04-03-2014 |
20140093929 | FATTY ALCOHOL FORMING ACYL REDUCTASES (FARS) AND METHODS OF USE THEREOF - The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same. | 04-03-2014 |
20140099686 | Production of Fatty Alcohols with Fatty Alcohol Forming Acyl-CoA Reductases (FAR) - The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as | 04-10-2014 |
20140099687 | PROCESSES FOR EXTRACTING CARBOHYDRATES FROM BIOMASS AND CONVERTING THE CARBOHYDRATES INTO BIOFUELS - A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step. | 04-10-2014 |
20140120594 | METHOD FOR REDUCING WATER USAGE IN A CELLULOSIC CONVERSION PROCESS - Disclosed herein is a process for recycling water in a cellulosic conversion process that comprises selecting process stream(s) that may contain reduced levels of inhibitors and subsequently subjecting the stream(s) to a treatment process to produce treated water. The treated water is thereafter recycled to the cellulosic conversion process, associated utilities or the seal water system. The process streams selected for treatment may comprise less than 5 wt % organic content and/or less than 5 wt % inorganic content. The process of the invention comprises at segregating process streams based on their treatment requirements. The segregated process streams may be sent to respective separate treatments selected from anaerobic digestion, aerobic digestion, physical separation and chemical treatment. | 05-01-2014 |
20140134690 | MICROBES AND METHODS FOR PRODUCING 1-PROPANOL - Provided herein are microbes metabolically engineered to produce 1-propanol from a 1,2-propanediol intermediate. The microbes may include one or two pathways for production of 1-propanol from a 1,2-propanediol intermediate. Also provided herein are methods for using the microbes for the production of 1-propanol. | 05-15-2014 |
20140134691 | Microorganisms for n-Propanol Production - Described herein are host cells comprising lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity, wherein the cells are capable of producing n-propanol. Also described are methods of producing n-propanol comprising (a) cultivating the host cells having lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity in a medium under suitable conditions to produce n-propanol; and (b) recovering the n-propanol. Methods of producing polypropylene from the recombinant n-propanol are also provided. | 05-15-2014 |
20140199742 | METHOD FOR PRODUCING ISOPROPYL ALCOHOL BY CONTINUOUS CULTURE - A method of producing isopropyl alcohol includes: culturing an isopropyl alcohol-producing | 07-17-2014 |
20140212940 | MEMBRANES - Mixed matrix pervaporation membranes are described which include i) a matrix phase comprising a polymeric material, and ii) a zeolitic imidazolate framework (ZIF) dispersed in the matrix phase. In membranes described, the thickness of the membrane is greater than 0.5 μm. The membranes may in examples be used in a process for separating an organic compound from an aqueous liquid mixture. An example process includes contacting the liquid mixture on one side of a mixed matrix pervaporation membrane to cause the organic compound to permeate the mixed matrix membrane, and removing from the other side of the membrane a permeate composition comprising a portion of the organic compound which permeated the membrane. Example membranes described have relatively good selectivity for separation of the organic compound from the liquid mixture. | 07-31-2014 |
20140248675 | METHYL BUTENOL SYNTHASE - The present invention provides novels genes encoding methyl butenol (MBO) synthase, methy butenol synthases and their use in methyl butenol production. | 09-04-2014 |
20140256008 | NEW BIOSYNTHESIS PATHWAY FOR PRENOL IN A RECOMBINANT MICROORGANISM - The present invention concerns a method for the biological preparation of prenol comprising culturing a microorganism genetically modified for the bioproduction of prenol, wherein the microorganism comprises a metabolic pathway for conversion of 3-methylcrotonyl-CoA into prenol by the action of an alcool dehydrogenase enzyme and of an aldehyde dehydrogenase enzyme. | 09-11-2014 |
20140256009 | METHOD FOR THE ENZYMATIC PRODUCTION OF BUTADIENE - Described is a method for the enzymatic production of butadiene which allows to produce butadiene from crotyl alcohol. Also described are enzyme combinations and compositions containing such enzyme combinations which allow the enzymatic conversion of crotyl alcohol into butadiene. Furthermore, the invention relates to microorganisms which have been genetically modified so as to be able to produce butadiene from crotyl alcohol. | 09-11-2014 |
20140273123 | Method for production of n-propanol and other C3-carbon containing products from syngas by symbiotic arrangement of C1-fixing and C3-producing anaerobic microorganism cultures - This invention provides methods and systems for the production of propanol. Specifically, the methods and systems of the present invention use symbiotic arrangement of anaerobic microorganism cultures for the production of propanol from syngas. | 09-18-2014 |
20140322776 | OVEREXPRESSION OF GENES THAT IMPROVE FERMENTATION IN YEAST USING CELLULOSIC SUBSTRATES - The invention relates to recombinant yeast host cells that overexpress proteins to improve glucose utilization, pentose sugar utilization and/or production of a fermentation product in a fermentation reaction. | 10-30-2014 |
20140342417 | CARBON-BASED DURABLE GOODS AND RENEWABLE FUEL FROM BIOMASS WASTE DISSOCIATION FOR TRANSPORTATION AND STORAGE - Techniques, systems, apparatus and material are described for generating renewable energy from biomass waste while sequestering carbon. In one aspect, a method performed by a reactor to dissociate raw biomass waste into a renewable source energy or a carbon byproduct or both includes receiving the raw biomass waste that includes carbon, hydrogen and oxygen to be dissociated under an anaerobic reaction. Waste heat is recovered from an external heat source to heat the received raw biomass waste. The heated raw biomass waste is dissociated to produce the renewable fuel, carbon byproduct or both. The dissociating includes compacting the heated raw biomass waste, generating heat from an internal heat source, and applying the generated heat to the compacted biomass waste under pressure. | 11-20-2014 |
20150017696 | RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A CROTONOL INTERMEDIATE - The present disclosure relates to recombinant host cells comprising one or more recombinant polynucleotides encoding enzymes in select pathways that provide the ability to use the cells to produce 1,3-butadiene. The present disclosure also provides methods of manufacturing the recombinant host cells, and methods for the use of the cells to produce 1,3-butadiene, either through formation of the intermediate compound crotonol followed by chemo-catalytic dehydration to 1,3-butadiene, or through the use of a recombinant cell comprising a fully enzymatic pathway capable of converting crotonyl-CoA or crotonyl-ACP to crotonol and then crotonol to 1,3-butadiene. | 01-15-2015 |
20150064757 | NOVEL CAR ENZYMES AND IMPROVED PRODUCTION OF FATTY ALCOHOLS - The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells. | 03-05-2015 |
20150093797 | PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS - Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids. | 04-02-2015 |
20150118725 | METHOD FOR THE ENZYMATIC PRODUCTION OF ISOPRENOL USING MEVALONATE AS A SUBSTRATE - Described is a method for generating isoprenol through a biological process. More specifically, described is a method for producing isoprenol from mevalonate. | 04-30-2015 |
20150132816 | MICROBIAL PRODUCTION OF BRANCHED MEDIUM CHAIN ALCOHOLS, SUCH AS 4-METHYLPENTANOL - The invention relates to recombinant cells and their use in the production of branched medium-chain alcohols such as 4-methyl-1-pentanol. | 05-14-2015 |
20150299732 | PRODUCTION OF ISOPRENOIDS - A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid. | 10-22-2015 |
20150361454 | ACYL-ACP REDUCTASE WITH IMPROVED PROPERTIES - The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics. | 12-17-2015 |
20150376653 | Process For The Production Of Isoprenol From Mevalonate Employing a Diphosphomevalonate Decarboxylae - Described is a method for the enzymatic production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol as well as the use of an enzyme which is capable of catalyzing the decarboxylation of mevalonate for the production of isoprenol from mevalonate. Furthermore described is the use of mevalonate as a starting material for the production of isoprenol in an enzymatically catalysed reaction. Also disclosed is a method for the production of isoprene comprising the method for the production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol and further comprising the step of converting the produced isoprenol into isoprene as well as a method for the production of isoamyl alcohol comprising the method for the production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol and further comprising the step of converting the produced isoprenol into isoamyl alcohol. | 12-31-2015 |
20160024489 | METHODS OF ISOLATING BACTERIAL STRAINS - Certain embodiments are directed to methods of developing bacterial strains having a selected metabolism for producing a target molecule(s) or bacterial strains or bacteriophage strains comprising a modified gene encoding a selected agent. | 01-28-2016 |
20160040138 | MODIFIED CARBONYL REDUCING ENZYME AND GENE - An object of the present invention is to modify a wild-type enzyme that is less reactive in the presence of an organic solvent to provide altered carbonyl reductases having better reactivity in the presence of the organic solvent than the wild-type enzyme, and/or to provide transformants producing such reductases. The present inventors have found altered carbonyl reductases having better reactivity in the presence of an organic solvent than the wild-type enzyme, from among a mutant enzyme library prepared by randomly mutating the wild-type enzyme gene, thereby arriving at completion of the present invention. | 02-11-2016 |
20160108436 | SYNTHESIS OF OLEFINIC ALCOHOLS VIA ENZYMATIC TERMINAL HYDROXYLATION - In certain aspects, the present invention provides methods for producing terminally hydroxylated alkenes and alkynes by contacting an unsaturated or saturated hydrocarbon substrate with a hydroxylase enzyme. Exemplary terminal hydroxylases useful for carrying out the methods of the invention exhibit strong selectivity towards one terminal carbon of a hydrocarbon substrate and include, but are not limited to, non-heme diiron alkane monooxygenases, cytochromes P450 (e.g., cytochromes P450 of the CYP52 and CYP153 family), as well as long chain alkane hydroxylases. In some embodiments, the terminally hydroxylated alkene or alkyne is further converted to a terminal alkenal. In certain embodiments, terminally hydroxylated alkenes and alkynes are useful as insect pheromones which modify insect behavior. In other embodiments, terminally hydroxylated alkenes and alkynes are useful intermediates for producing pheromones via acetylation or oxidation of the alcohol moiety. | 04-21-2016 |
20160145098 | System And Method For Producing A Consistent Quality Syngas From Diverse Waste Materials With Heat Recovery Based Power Generation, And Renewable Hydrogen Co-Production - A system and method for converting waste and secondary materials into synthesis gas (syngas) through the use of a molten metal bath gasifier for the initial breakdown of waste feeds and an A/C plasma reactor for complete dissociation of waste feeds into syngas, and an anaerobic digester. The system includes a heat recovery and steam power generation process for the production of electricity. The system produces a net output of electricity above plant load sufficient for the co-production of renewable Hydrogen and Oxygen. The process does not require the use of fossil fuels or fossil feedstocks during normal operations, and it eliminates combustion produced stack emissions or landfill residuals. | 05-26-2016 |
20160160204 | MEVALONATE DIPHOSPHATE DECARBOXYLASE VARIANTS - Described are mevalonate diphosphate decarboxylase variants having improved activity in converting 3-phosphonoxyisovalerate into isobutene. Such variants can be employed in processes for biologically producing isobutene from 3-hydroxyisovalerate or from 3-hydroxy-3-methylbutyrate into isobutene, for biologically producing isoprenol from mevalonate or from mevalonate-3-phosphate or for biologically producing 1,3-butadiene from 3-hydroxypent-4-enoate or from 3-phosphonoxypent-4-enoate. | 06-09-2016 |
20160251633 | RECOMBINANT YEAST AND SUBSTANCE PRODUCTION METHOD USING THE SAME | 09-01-2016 |