Entries |
Document | Title | Date |
20080213891 | RNAi Agents Comprising Universal Nucleobases - One aspect of the present invention relates to an oligonucleotide agent comprising at least one universal nucleobase. In certain embodiments, the universal nucleobase is difluorotolyl, nitroindolyl, nitropyrrolyl, or nitroimidazolyl. In a preferred embodiment, the universal nucleobase is difluorotolyl. In certain embodiments, the oligonucleotide is double-stranded. In certain embodiments, the oligonucleotide is single-stranded. Another aspect of the present invention relates to a method of altering the expression level of a target in the presence of target sequence polymorphism. In a preferred embodiment, the oligonucleotide agent alters the expression of different alleles of a gene. In another preferred embodiment, the oligonucleotide agent alters the expression level of two or more genes. In another embodiment, the oligonucleotide agent alters the expression level of a viral gene from different strains of the virus. In another embodiment, the oligonucleotide agent alters the expression level of genes from different species. | 09-04-2008 |
20080213892 | SELF-RENEWAL OF NEURAL STEM CELLS IS PROMOTED BY WNT PROTEINS - Mammalian neural progenitor or stem cells are expanded in vitro by culture in the presence of one or more wnt polypeptides. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. | 09-04-2008 |
20080220521 | SHEET FOR GUIDING REGENERATION OF MESENCHYMAL TISSUE AND PRODUCTION METHOD THEREOF - To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell. | 09-11-2008 |
20080227202 | Method of promoting differentiation of one or more human stem cells into human coronary endothelial cells on a synthetic tubular structure - A method of promoting differentiation of one or more human stem cells into human coronary endothelial cells on at least one surface of a synthetic tubular structure to be used to make a human hybrid hemodialysis access graft is provided. The method includes arranging a plurality of human stem cells on the synthetic tubular structure to yield a hybrid stem cell/synthetic tubular structure and subjecting ex vivo, the hybrid stem cell/synthetic tubular structure to three dimensional dynamic conditions effective to promote differentiation of the one or more human stem cells into human coronary endothelial cells on the at least one surface. | 09-18-2008 |
20080233648 | BIASING OF CELLS TOWARD RETINAL, CORNEAL OR LENS DEVELOPMENT - Methods are described that bias cells, such as potent and multipotent stem cells, by transfection with a nucleic acid sequence, to differentiate to a desired end-stage cell or a cell having characteristics of a desired end-stage cell. In particular embodiments, human neural stem cells or mesenchymal stem cells are transfected with vectors comprising genes in the homeobox family of transcription factor developmental control genes, and this results in a greater percentage of resultant transformed cells, or their progeny, differentiating into a desired end-stage cell or a cell having characteristics of a desired end-stage cell. | 09-25-2008 |
20080248570 | Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of cells - A method is provided of propagating hepatic cells including hepatic progenitors ex vivo on or in hyaluronans with or without other extracellular matrix components (such as collagens, basal adhesion molecules, proteoglycans or their glycosaminoglycans) and with or without hormones and/or growth factors. Compositions comprising the matrix are also disclosed. Also, the complex can be used for ex vivo tissue engineering or can be used as a scaffold for grafts of cells to be transplanted in vivo. | 10-09-2008 |
20080254538 | Treatment of Neurological Deficits in the Striatum or Substanta Nigra Pars Compacta - The present invention is directed to methods of treating neurological deficits resulting from injury or disease to the striatum or substanta nigra pars compacta of a human by administering BMP7 to the striatum or substanta nigra pars compacta of a human in amounts effective to induce cell populations having the capacity to differentiate towards a dopaminergic phenotype to in fact differentiate towards a dopaminergic phenotype, and to neurotrophic compositions and matrices suitable for use in such treatments. | 10-16-2008 |
20080286867 | Method of Inducing Embryonic Stem Cells Into Pancreatic Cells - The present invention provided a simple three-step approach based on the combinational induction with activin A, all-trans retinoic acid and, optionally, other maturation factors which are able to induce embryonic stem cells to differentiate into insulin-producing cells. A kit used to induce embryonic stem cells to differentiate into insulin-producing cells was also provided. | 11-20-2008 |
20080293137 | COMPOUNDS THAT ABROGATE DNA DAMAGE INDUCED CELL CYCLE G2 CHECKPOINT AND/OR AUGMENT ANTI-CANCER ACTIVITY OF DNA-DAMAGING TREATMENTS - The invention provides compositions and methods to inhibit the cell cycle G2 checkpoint, in particular the DNA-damage-induced G2 checkpoint, in mammalian cells including human cells. Specifically, the invention provides compositions and methods to sensitize cells to DNA-damaging agents by abrogating the cell cycle G2 checkpoint. Compounds of the invention are used to treat proliferative disorders such as cancer. The invention provides compositions and methods for selectively sensitizing G1 checkpoint impaired cancer cells to DNA-damaging agents and treatments. | 11-27-2008 |
20080305544 | Method of Producing Nerve Cell - An Object of the present invention is to provide a process for producing a nerve cell by inducing differentiation of an embryonic stem cell, a method for inducing differentiation of the embryonic stem cell into a nerve cell, a medium to be used in the production process or differentiation induction method, or a method for improving purity of the nerve cell obtained by inducing differentiation of the embryonic stem cell. The present invention provides a process for producing a nerve cell which is applicable to treatment of neurodegenerative disease or the like easily, selectively or inexpensively by inducing differentiation induction of an embryonic stem cell using vitamin B | 12-11-2008 |
20080305545 | Shape memory thermoplastics and polymer networks for tissue engineering - Methods and compositions are described herein for reconstruction of different functional tissues. Dissociated cells, differentiated cells, adult mesenchymal stem cells or embryonic stem cells are seeded on a scaffold. The scaffold will consist of a biocompatible, biodegradable shape memory (“SM”) polymers. In addition bioactive substances may be incorporated in the scaffold. Thermoplastic as well as thermoset materials with SM-effect can be used. The shape memory effect will be applied as an interactive link between the cells and the used polymeric scaffold. The degradation kinetics as well as shape memory transition temperature will be tailored by adjusting to monomer ratios of the co-oligomers. The shape memory effect will be used to create a degradation or release of bioactive substances on demand, induce forces on seeded cells or induce proliferation and differentiation of cells. | 12-11-2008 |
20080305546 | Method for cultivating tendon cells from pluripotent cells of mesenchymal origin - A method for cultivating tendon cells from non-embryonic pluripotent cells of mesenchymal origin is described, wherein the isolated cells are cultivated in a culture medium under standard culture conditions in a culture vessel. In order to increase the collagen secretion, it is proposed that before their complete confluence, the cells are on the one hand further cultivated in a culture medium mixed with ascorbic acid and/or ascorbic acid-2-phosphate in a concentration of 25 to 75 μg/ml and on the other hand, subjected to hyperosmolar treatment in a culture medium whose osmolarity is adjusted to 350 to 500 mosmol/l. | 12-11-2008 |
20080318315 | Reverse-Flow Perfusion of Three-Dimensional Scaffolds - The invention is a reverse-flow method and system for the loading, proliferation and differentiation of cells into and throughout an implantable biocompatible three-dimensional scaffold. | 12-25-2008 |
20080318316 | Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same - A method is provided for controlling the survival, proliferation, and/or differentiation of hepatic progenitors in vitro by using specific types of mesenchymal feeder cells or one of more of the paracrine signals produced by those feeders. | 12-25-2008 |
20090004736 | GENERATION OF NEURAL STEM CELLS UNDIFFERENTIATED HUMAN EMBRYONIC STEM CELLS - The present invention relates to the generation of neural cells from undifferentiated human embryonic stem cells. In particular it relates to directing the differentiation of human ES cells into neural progenitors and neural cells and the production of functioning neural cells and/or neural cells of a specific type. The invention also includes the use of these cells for the treatment of neurological conditions such as Parkinson's disease. | 01-01-2009 |
20090017539 | METHODS AND MEANS FOR STEM CELL PROLIFERATION AND SUBSEQUENT GENERATION AND EXPANSION OF PROGENITOR CELLS, AS WELL AS PRODUCTION OF EFFECTOR CELLS AS CLINICAL THERAPEUTICS - The invention provides means and methods for stem cell proliferation and subsequent generation and expansion of progenitor cells. The invention in particular provides media and other culture conditions for the same. The cells are preferably used as effector cells as clinical therapeutics. | 01-15-2009 |
20090029469 | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens - A method for producing proliferating cultures of dendritic cell precursors is provided. Also provided is a method for producing mature dendritic cells in culture from the proliferating dendritic cell precursors. The cultures of mature dendritic cells provide an effective means of producing novel T cell dependent antigens comprised of dendritic cell modified antigens or dendritic cells pulsed with antigen, including particulates, which antigen is processed and expressed on the antigen-activated dendritic cell. The novel antigens of the invention may be used as immunogens for vaccines or for the treatment of disease. These antigens may also be used to treat autoimmune diseases such as juvenile diabetes and multiple sclerosis. | 01-29-2009 |
20090029470 | PROCESS AND CULTURING UNIT FOR CULTURING BIOLOGICAL CELLS - A targeted cultivation phase adjustment is provided in a process for the cultivation of cells in which biological cells are cultivated on at least one boundary surface between different, non-solid cultivation phases so that pre-determined cultivation conditions are given. A cultivation apparatus ( | 01-29-2009 |
20090035855 | THREE-DIMENSIONAL RECONSTITUTED EXTRACELLULAR MATRICES AS SCAFFOLDS FOR TISSUE ENGINEERING - A biomaterial scaffold comprising: a) reconstituted extracellular matrix; and b) polyelectrolyte complex fibers; wherein the matrix and the fibers are functionally associated. | 02-05-2009 |
20090047739 | Direct Differentiation Method for Making Cardiomyocytes from Human Embryonic Stem Cells - This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of select factors and morphogens. After enrichment for cells with the appropriate phenotype, the cells are allowed to cluster into Cardiac Bodies™, which are remarkably homogeneous and suitable for the treatment of heart disease. | 02-19-2009 |
20090053809 | Retina-Specific Cells Differentiated In Vitro from Bone Marrow Stem Cells, the Production Thereof and Their Use - The invention relates to the production of retina-specific cells from human adult bone marrow stem cells by culturing bone marrow stem cells in the presence of a differentiation medium. The invention also relates to retina-specific cells and to the use of these cells for treating diseases associated with acquired or congenital dysfunction of the retinal pigment epithelium, cells of adjacent structures of the entire retina and of the choroid coat as well as of other eye tissue. | 02-26-2009 |
20090081791 | INTRACELLULAR DELIVERY OF SMALL MOLECULES, PROTEINS, AND NUCLEIC ACIDS - An amino acid sequence Arg-Lys-Met-Leu-Lys-Ser-Thr-Arg-Arg-Gln-Arg-Arg (SEQ ID NO:1) functions as a protein transduction domain (PTD) and is capable of delivering small molecules, proteins, and nucleic acids to an intracellular compartment of a cell. An amino terminal lysine linker improves the efficiency of the PTD. A nuclear localization signal can be used to target the PTD to a cell's nucleus. The PTD can be used in PTD-cargo moiety complexes that can reversibly immortalize cells and increase cell viability in culture. | 03-26-2009 |
20090104697 | Method of differentiation of morula or inner cell mass cells and method of making lineage-defective embryonic stem cells - An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell. | 04-23-2009 |
20090117651 | Nucleoside compounds and methods of use thereof - The present invention provides methods of utilizing a nucleoside derivative having the chemical formula of Formula (I) to downregulate expression of an anti-apoptotic protein such as survivin in a cell, induce apoptosis in a cell, inhibit angiogenesis in a cell, inhibit binding of p53 to DNA in a cell, inhibit phosphorylation of Akt in a cell and inhibit HIV transcription in a cell, by administering to the cell or tissue an amount of a compound of Formula (I) sufficient to achieve the desired activity. Formula (I): wherein the substituents R | 05-07-2009 |
20090117652 | USE OF CXCL6 CHEMOKINE IN THE PREVENTION OR REPAIR OF CARTILAGE DEFECTS - The present invention disclosed the expression of CXCL6 by cells which are able to form stable cartilage. The invention describes the use of these cells and of CXCL6 to promote cartilage (and underlying bone) formation e.g. in the repair of cartilage or osteochondral defects. The invention further describes the use of chemokines in the modulation of progenitor cell differentiation. | 05-07-2009 |
20090124009 | ANTISENSE MODULATION OF PTP1B EXPRESSION - Compounds, compositions and methods are provided for modulating the expression of PTP1B. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding PTP1B. Methods of using these compounds for modulation of PTP1B expression and for treatment of diseases associated with expression of PTP1B are provided. | 05-14-2009 |
20090142833 | Pine Cone Extracts and Uses Thereof - A method of producing a pine cone extract and the pine cone extract produced there from, wherein the pine cone extract is useful in increasing the effects of nucleic acid vaccines and medicaments; and useful in the production of phenotypically immature and/or mature dendritic and/or fibrocyte cells. | 06-04-2009 |
20090162934 | MULTIPLE MESODERMAL LINEAGE DIFFERENTIATION POTENTIALS FOR ADIPOSE TISSUE-DERIVED STROMAL CELLS AND USES THEREOF - The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair. | 06-25-2009 |
20090170197 | Nucleic Acid-Mediated Treatment of Diseases or Conditions Related to Levels of Vascular Endothelial Growth Factor Receptor (VEGF-R) - The present invention relates to nucleic acid molecules such as ribozymes, DNAzymes, short interfering RNA (siRNA), short interfering nucleic acid (siNA), and antisense which modulate the synthesis, expression and/or stability of an mRNA encoding one or more receptors of vascular endothelial growth factor, such as flt-1 (VEGFR1) and/or KDR (VEGFR2). Nucleic acid molecules and methods for the inhibition of angiogenesis and treatment of cancer and other conditions associated with VEGF-R are provided, optionally in conjunction with other therapeutic agents such as interferons. | 07-02-2009 |
20090170198 | Differentiation of human embryonic stem cells - The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells. | 07-02-2009 |
20090176306 | METHOD OF INDUCING DIFFERENTIATION FROM VISCERAL PREADIPOCYTE TO VISCERAL ADIPOCYTE - The invention provides a method of inducing differentiation of a visceral preadipocyte and a method of culturing a visceral adipocyte comprising culturing the cell in a medium containing serum free of heparin-adsorbable components. The invention also provides for the removal of heparin-adsorbable components by, for example, applying serum to a heparin affinity column thereby adsorbing the heparin-adsorbable component to heparin. A visceral adipocyte cultured by the method of the present invention is useful in a study of differentiation, growth and metabolism of an adipocyte, elucidation of the mechanism of development and progress of diseases such as diabetes, hyperlipemia, hypertension and arteriosclerosis, and development of a drug for preventing and treating such a disease. | 07-09-2009 |
20090176307 | Compounds, Compositions and Methods - Certain substituted urea derivatives selectively modulate the cardiac sarcomere, for example by potentiating cardiac myosin, and are useful in the treatment of systolic heart failure including congestive heart failure. | 07-09-2009 |
20090186411 | Cell culture apparatus, method for producing the apparatus and cell culture method - The invention relates to a cell culture apparatus for cells, characterized by a surface composed of an unstructured elastomer. It is thus possible for cells to be cultured under close to natural conditions in relation to their environmental elasticity. A method for producing an apparatus according to the invention is disclosed, as is a cell culture method using such an apparatus. | 07-23-2009 |
20090197333 | METHOD FOR PRODUCTION OF BIOLOGICAL ORGANIC MATERIAL AND CULTURE VESSEL - Disclosed is a method for producing a biological organic material in a liquid droplet comprising a culture medium composed of specified ingredient. The method comprises the steps of: providing a substantially spherical liquid droplet on a substrate in an unadhered state, the substrate preferably having such a water-repellent surface that the water contact angle becomes 150° or greater; and culturing a biological organic material in the liquid droplet. | 08-06-2009 |
20090197334 | METHODS OF CULTURING EMBRYONIC STEM CELLS AND CONTROLLED DIFFERENTIATION - The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention. | 08-06-2009 |
20090215175 | MODULATION OF THE POLIOVIRUS RECEPTOR FUNCTION - The present invention relates to the identification, the isolation and the use of molecules interfering with the function (s) mediated by the poliovirus receptor (PVR) on cells. The molecules can be used for the treatment of cells having a metastatic potential, metastasis and cancer. Further methods are provided that are useful for identifying and isolating molecules, which have the capacity to modulate PVR mediated adhesion or invasion potential of cells. | 08-27-2009 |
20090221072 | COMPOSITIONS AND METHODS FOR MODULATING CELL DIFFERENTIATION - Compositions and methods are described for using the Ea4-peptide of pro-IGF-I or human Eb-peptide of pro-IGF-I to inhibit hematopoiesis and to induce differentiation of neuroblastoma cells and neuronal stem cells. | 09-03-2009 |
20090239300 | SEPARATING MATERIAL AND METHOD FOR COLLECTING CELL OR THE LIKE USING THE SAME - The present invention provides a novel material useful for selectively isolating a cell such as monocyte and the like or a protein from a body fluid and a production method thereof, a physiological material using the material and an isolation material using the physiological material, as well as a method of harvesting a cell such as monocyte and the like using the isolation material, a method of harvesting a protein and a method of preparing a dendritic cell. | 09-24-2009 |
20090239301 | USE OF VINCA ALKALOIDS AND SALTS THEREOF - An agent containing a vinca alkaloid or its pharmacologically acceptable salt as an active ingredient can induce insulin production and/or secretion of non-neoplastic cells derived from the pancreas. | 09-24-2009 |
20090246870 | DE-DIFFERENTIATION OF ASTROCYTES INTO NEURAL STEM CELL USING NANOG - Disclosed are a composition and a method for inducing the de-differentiation of astrocytes into neural stem cells using Nanog. The de-differentiated neural stem cells have the ability to differentiate into astrocytes, neurons, or oligodendrocytes. | 10-01-2009 |
20090246871 | CONTAINER FOR GERM LAYER FORMATION AND METHOD OF FORMING GERM LAYER - The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies. | 10-01-2009 |
20090253203 | Reprogramming a Cell by Inducing a Pluripotent Gene Through Use of a Small Molecule Modulator - The invention relate to methods, compositions, and kits for reprogramming a cell. In one embodiment, the invention relates to a method comprising inducing the expression of at least one gene that contributes to a cell being pluripotent or multipotent. In yet another embodiment, the method comprises exposing a cell to a small molecule modulator that induces the expression of at least one gene that contributes to a cell being pluripotent or multipotent. In yet another embodiment, the invention relates to a reprogrammed cell and an enriched population of reprogrammed cells that can have characteristics of an ES-like cell can be re- or trans-differentiated into various differentiated cell types. | 10-08-2009 |
20090258423 | Cell cycle regulation and differentiation - The present invention provides compositions and methods for regulating neural cell proliferation or differentiation. The present invention also provides methods for selecting for bioactive agents effective in regulating proliferation or differentiation. | 10-15-2009 |
20090263897 | REISHI F3 SUB FRACTION POLYSACCHARIDES AND METHODS OF USING SAME - The present disclosure relates to the discovery of methods of isolating subfractions of an F3 Reishi extract, and of administration of these novel isolates to eukaryotic cells in order to induce certain immumodulatory, hematopoeitic and tumor-inhibiting phenotypic changes in those eukaryotic cells, mediated through particular toll-like receptor (TLR) and other transmembrane receptors. F3 subfractions F301 and F331 have demonstrated that F331 is capable of activating at least TLR-2 while F301 is capable of activating at least TLR-2, TLR-4, and TLR-5. | 10-22-2009 |
20090269847 | SELF-ASSEMBLING PEPTIDE AMPHIPHILES AND RELATED METHODS FOR GROWTH FACTOR DELIVERY - Amphiphilic peptide compounds comprising one or more epitope sequences for binding interaction with one or more corresponding growth factors, micellar assemblies of such compounds and related methods of use. | 10-29-2009 |
20090280564 | METHOD FOR INDUCING DIFFERENTIATION OF MESODERMAL STEM CELLS, ES CELLS, OR IMMORTALIZED MESODERMAL STEM CELLS INTO NEURAL CELLS - Mesodermal stem cells or ES cells, prepared from the mononuclear cell fraction isolated from bone marrow fluid or umbilical blood, were found to differentiate into neural stem cells, neurons, or glial cells when cultured in a basal culture medium. In addition, the differentiation of the mesodermal stem cells or ES cells into neural cells was promoted through the addition of an ischemic brain extract to the above-mentioned basal culture medium. Furthermore, the neural cells obtained using the above-described method for inducing differentiation were revealed to have neural regeneration potency in a brain infarction model, a dementia model, a spinal cord injury model and a demyelination model. | 11-12-2009 |
20090286317 | MODULAR CULTURE SYSTEM FOR MAINTENANCE, DIFFERENTIATION AND PROLIFERATION OF CELLS - The invention provides a culture system, which combines a device for undifferentiated proliferation of the cells with a device for cell differentiation and proliferation. Aspects of the invention include a modular culture system, a kit and an associated method for combining undifferentiated proliferation of cells with differentiation and proliferation of cells in a single, integrated device. The culture system has a first compartment comprising a plurality of miniaturized culture cavities that are sealed on one end by a surface that may be perforated, and a second compartment which defines one or more, larger culture chambers, and wherein said first and second compartment can be sterilely connected and living cell material can be transferred directly and in a controlled manner from the first compartment into the second compartment. | 11-19-2009 |
20090298178 | GROWTH FACTORS FOR PRODUCTION OF DEFINITIVE ENDODERM - Disclosed herein are methods for generating endoderm lineage type cells derived from human pluripotent cells, such as human embryonic stem cells, by using various agents including, but not limited to, GDF8, GDF11 and GSK-3beta inhibitors. Also disclosed herein are endoderm lineage cell populations or compositions, such as populations or compositions comprising definitive endoderm and/or other definitive endoderm-derived cell types. | 12-03-2009 |
20090298179 | Methods For Treating Degenerative Diseases/Injuries - Invented is a method of treating degenerative diseases/injuries, in a mammal, including a human, in need thereof which comprises the administration of a therapeutically effective amount of a non-peptide TPO receptor agonist to such mammal. | 12-03-2009 |
20090305411 | RECOMBINANT ANTIBODIES AND IMMUNOCONJUGATES TARGETED TO CD-22 BEARING CELLS AND TUMORS - Methods and compositions relating to anti-CD22 antibodies with high binding affinity, and immunoconjugates comprising the anti-CD22 antibody linked to a therapeutic agent such as a | 12-10-2009 |
20090325293 | TREATMENT OF PLURIPOTENT CELLS - The present invention is directed to methods to treat pluripotent cells, whereby the pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of GSK-3B enzyme activity. | 12-31-2009 |
20090325294 | SINGLE PLURIPOTENT STEM CELL CULTURE - The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels. | 12-31-2009 |
20090325295 | IN-VITRO MECHANICAL LOADING OF MUSCULOSKELETAL TISSUES - Musculoskeletal tissues produced in vitro are optimized in response to an externally applied mechanical load. The load applied may vary from tissue to tissue, depending upon the response desired, and may include intermittent axial, torsional, and bending loads to produce cortical structures. Compression alone is preferably applied to produce cancellous bone. A method according to the invention for culturing bone in vitro comprises: providing a culture vessel providing a scaffold material, supporting the scaffold material within the tissue culture vessel so as to be exposed to a tissue culture medium, and exerting a force on the scaffold material during growth of a bone construct (a cultured bone growth) around the scaffold material. Applicable apparatus preferably includes a culture vessel, holders for holding a scaffold within the tissue culture vessel, means for introducing a tissue culture medium to the tissue culture vessel, and an actuator adapted to apply a force to developing bone during the in vitro culture of the tissue, whether bone, cartilage, ligament, or composites thereof. | 12-31-2009 |
20100003753 | Multivalent rna nanoparticles for delivery of active agents to a cell - A polyvalent multimeric complex formed from a plurality of chimeric pRNA molecules, each carrying at least one biologically active moiety, detectable label, or other heterologous component. | 01-07-2010 |
20100003754 | METHODS OF DIFFERENTIATING STEM CELLS - A method for at least partial differentiating stem cells and/or progenitor cells to at least one tissue type includes treating the stem cells and/or the progenitor cells with extracts which include active substances and/or components for differentiating stem cells and/or progenitor cells, and cultivating and differentiating the stem cells and/or the progenitor cells. | 01-07-2010 |
20100009447 | Vgamma9Vdelta2 T cell proliferation agent, method for producing activated Vgamma9Vdelta2 T cells, and uses thereof - A Vγ9Vδ2 T cell proliferation agent includes at least a bisphosphonate, interleukin 2, and interleukin 18. Since has properties that improve cell viability by inhibiting cell death, IL-18 is presumably capable of acting as a cofactor for the bisphosphonate so as to significantly increase the effect of Vγ9Vδ2 T cell proliferation by the bisphosphonate and the IL-2. This allows providing a Vγ9Vδ2 T cell proliferation agent capable of growing Vγ9Vδ2 T cells with a proliferated efficiency significantly high compared to conventional methods so that the proliferated Vγ9Vδ2 T cells have a high antitumor activity and high cytokine productivity. | 01-14-2010 |
20100015709 | Regulating Stem Cell Differentiation By Controlling 2D and 3D Matrix Elasticity - Provided are methods for the selection and regulation of the mechanical properties of 2D or 3D biocompatible substrates or tissue microenvironments as a technique to regulate in vitro differentiation, cell shape and/or lineage commitment of anchorage-dependent cells, such as mesenchymal stem cells into, e.g., neurogenic-, myogenic-, and osteogenic-type cells. Substrate mechanical properties include elasticity, tension, adhesion, and myosin-based contractile mechanisms. Inhibitors can be introduced to further regulate differentiation. | 01-21-2010 |
20100015710 | Methods and Compositions for Isolating, Maintaining and Serially Expanding Human Mesenchymal Stem Cells - Compositions and methods for isolating and expanding human mesenchymal stem/progenitor cells through multiple passages in defined serum-free environments are provided. The culture media compositions includes a basal medium supplemented with a nutrient mixture such as Ham's F12 nutrient mixture, glutamine, buffer solutions such as sodium bicarbonate and hepes, serum albumin, a lipid mixture, insulin, transferrin, putrescine, progesterone, fetuin, hydrocortisone, ascorbic acid or its analogues such as ascorbic acid-2-phosphate, fibroblast growth factor and transforming growth factor β, and are free of serum or other undefined serum substitutes such as platelet lysate. Methods employing these compositions and protein-coated surfaces for the isolation of mesenchymal stem/progenitor cells from human bone marrow and other tissues such as adipose tissue are also provided. Finally, methods are also provided for serially expanding these cells through multiple passages without losing mesenchymal stem cell-specific proliferative, phenotypical and differentiation characteristics. | 01-21-2010 |
20100015711 | Differentiation of Pluripotent Stem Cells - The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. | 01-21-2010 |
20100022003 | Therapeutic cell medicine comprising skin tissue derived stem cell - Provided is a cell therapeutic agent for treatment of neurological disorders, comprising skin-derived progenitor cells (SPCs). More specifically, the present invention provides a cell therapeutic agent for treatment of neurological disorders, comprising skin-derived progenitor cells (SPCs) isolated from skin tissues and a method for differentiation of the skin-derived progenitor cells (SPCs) into neural cell lineages. The cell therapeutic agent in accordance with the present invention is therapeutically effective for the treatment of the neurological disorders and diseases such as Parkinson's disease, Alzheimer's disease, Huntington's disease and Amyotrophic Lateral Sclerosis (ALS) caused by neural injury, and neurological deficits due to cerebral apoplexy, ischemia and spinal cord injury. Further, the present invention enables transplantation of autologous cells to thereby minimize adverse side effects. | 01-28-2010 |
20100028999 | METHODS, APPARATUS, AND SYSTEMS FOR FABRICATION OF POLYMERIC NANO- AND MICRO-FIBERS IN ALIGNED CONFIGURATIONS - Provided herein are apparatus and systems for fabricating highly aligned arrays of polymeric fibers having isodiameters ranging from sub 50 nm to microns with lengths of several millimeters. The approach disclosed herein uses (e.g.) a micropipette to deliver polymeric solution which is collected in the form of aligned fibers on a rotating and linearly translating substrate. The methods deposit polymeric fibers on spherical surfaces and gapped surfaces with precise control, thus heralding new opportunities for a variety of applications employing polymeric fibers. The design workspace for depositing fibers disclosed herein is dependent upon processing parameters of rotational/linear translational speeds and material properties of solution rheologies. Techniques for fabrication of multilayer fiber arrays, for fabrication of cell growth scaffolds and for attachment of particles to the fiber arrays are also disclosed. | 02-04-2010 |
20100035341 | Human Embryonic Stem Cell-Derived Connective Tissue Progenitors For Tissue Engineering - Methods of generating and expanding proliferative, multipotent connective tissue progenitor cells from embryonic stem cells and embryoid bodies are provided. Also provided are methods of generating functional tendon grafts in vitro and bone, cartilage and connective tissues in vivo using the isolated cell preparation of connective tissue progenitor cells. | 02-11-2010 |
20100041145 | TOLEROGENIC DENDRITIC CELLS, METHOD FOR THEIR PRODUCTION AND USES THEROF - The present invention relates to a tolerogenic dendritic cell population (Tr-DC) capable of generating a population of T cells having regulatory activity, method of production and uses thereof. Furthermore, soluble HLA-G promotes the differentiation of a population of T cells with regulatory activity. | 02-18-2010 |
20100047911 | COMPOSITIONS AND METHODS FOR ISOLATION, PROPAGATION, AND DIFFERENTIATION OF HUMAN STEM CELLS AND USES THEREOF - The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders. | 02-25-2010 |
20100055789 | METHOD AND APPARATUS FOR THE STIMULATION OF HAIR GROWTH - A method for activating or inhibiting the differentiation of stem cells including exposing the stem cells to a source of narrowband multi chromatic electromagnetic radiation under conditions effective to activate or inhibit cell differentiation. | 03-04-2010 |
20100068810 | METHODS AND COMPOSITIONS FOR GROWTH OF EMBRYONIC STEM CELLS - The present invention provides methods and compositions for establishing and maintaining growth of undifferentiated stem cells. In particular, the present invention provides synthetic growth matrices for stem cells, wherein said cells are capable of going through multiple passages while remaining in an undifferentiated state. | 03-18-2010 |
20100081199 | Generation of mature myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+progenitors - A method for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, dendritic cells an Langerhans cells from human embryonic stem cells is disclosed. | 04-01-2010 |
20100081200 | FORMULATIONS AND METHODS FOR CULTURING STEM CELLS - The present invention relates to a serum replacement formulation and to a culture medium suitable for the derivation, maintenance and differentiation of stem cells. | 04-01-2010 |
20100087001 | ANTI-ANGIOGENIC CELLULAR AGENT FOR CANCER THERAPY - The invention provides cytokine induced killer (CIK) cell populations and methods of using CIK cells to treat cellular proliferative disorders. CIK cells generated in vitro include both bulk cultures and clones. Individual CIK cell clones display distinct but overlapping lytic specificities for tumor cells and endothelial cells in vitro. When injected in vivo, bulk CIK cell cultures selectively attack tumor tissue. CIK cells can be used to treat a variety of cellular proliferative disorders, including early and late stage cancers as well as hematopoietic cell and solid tissue tumors. | 04-08-2010 |
20100093088 | RETINOID COMPOUNDS AND THEIR USE - The invention relates to retinoid compounds of the formula (I): | 04-15-2010 |
20100093089 | DEDIFFERENTIATION OF ADULT MAMMALIAN CARDIOMYOCYTES INTO CARDIAC STEM CELLS - Dedifferentiation is a mechanism whereby specialized cells regain properties of their ancestors, including, in the extreme, stemness. We found that highly-purified cardiomyocytes isolated from adult mammalian hearts dedifferentiated rapidly when cultured in mitogen-rich medium. Such myocytes reentered the cell cycle and proliferated, expressing stem cell surface markers such as c-kit and early cardiac transcription factors including GATA and NKx2.5. These myocyte-derived cells (MDC) were capable of re-differentiating into myocytes and endothelial cells. Contrary to prevailing dogma, cardiomyocyte dedifferentiation yields proliferative cells expressing stem cell markers and capable of multilineage differentiation. Cardiomyocyte dedifferentiation is a potential source of endogenous stem cells in the adult heart. | 04-15-2010 |
20100093090 | METHOD AND KIT FOR EFFICIENT REPROGRAMMING OF SOMATIC CELLS - The present invention relates to method or kit for efficient reprogramming of somatic cells. | 04-15-2010 |
20100093091 | Undifferentiated Stem Cell Culture Systems - The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal™ medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters. | 04-15-2010 |
20100093092 | METHOD FOR INDUCING PLURIPOTENCY IN CELLS - The present application describes a method for inducing or maintaining pluripotency in a cell by contacting the cell with a biological or chemical species that increases MUC1* activity. | 04-15-2010 |
20100105137 | METHOD FOR INDUCTION/DIFFERENTIATION INTO PHOTORECEPTOR CELL - The present invention provides a method of producing primate retinal progenitor cells, comprising culturing primate embryonic stem cells as suspended aggregates in a serum-free medium, and obtaining retinal progenitor cells from the culture. The present invention further provides a method of producing photoreceptor precursor cells, comprising culturing isolated retinal progenitor cells differentiated from embryonic stem cells, under adhesive conditions, in the presence of a gamma secretase inhibitor, and obtaining a photoreceptor precursor from the culture. | 04-29-2010 |
20100112691 | Stem Cell Aggregate Suspension Compositions and Methods of Differentiation Thereof - The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof. | 05-06-2010 |
20100112692 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides a method to increase the expression of markers associated with the pancreatic endocrine lineage. | 05-06-2010 |
20100112693 | Differentiation of Human Embryonic Stem Cells - The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependant kinase inhibitor to cause an increase in expression of MAFA. | 05-06-2010 |
20100136688 | METHOD OF DIFFERENTIATING MAMMALIAN PROGENITOR CELLS INTO INSULIN PRODUCING PANCREATIC ISLET CELLS - The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells. | 06-03-2010 |
20100159595 | METHOD OF GENERATING MYELINATING OLIGODENDROCYTES - A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFRα+. | 06-24-2010 |
20100159596 | SMALL MOLECULE COMPOUNDS FOR STEM CELL DIFFERENTIATION - Methods and small molecule compounds for stem cell differentiation are provided. One example of a class of compounds that may be used is represented by the compound having the structure IA or IB in the form of free base or a pharmaceutically acceptable salt, hydrate, solvate or N-oxide thereof: | 06-24-2010 |
20100173414 | Methods of Deriving Differentiated Cells from Stem Cells - The present invention provides a method of generating definitive endoderm, mesoderm, or ectoderm cells. The method includes culturing embryonic stem cells, parthenogenetic cells, or induced pluripotent stem cells in the presence of a demethylation agent, a histone deacetylase inhibitor, or a combination thereof, and thereafter, culturing the stem cells in the absence of the agent or combination of agents, to produce definitive endoderm cells, mesoderm, or ectoderm cells. | 07-08-2010 |
20100178700 | NOVEL THYMIC CELLULAR POPULATIONS AND USES THEREOF - The present invention relates generally to novel thymic cellular populations and, more particularly, to novel thymic epithelial cellular populations. Most particular, the present invention is directed to novel thymic epithelial progenitor cell populations. The cellular populations of the present invention are useful in a wide range of clinical and research settings including, inter alia, the in vitro or in vivo generation of thymic epithelial cell populations and the therapeutic or prophylactic treatment of a range of conditions via the administration of these cells. Also facilitated is the design of in vitro based screening systems for testing the therapeutic impact and/or toxicity of potential treatment or culture regimes to which thymic epithelial cells may be exposed. In another aspect, the present invention is directed to a method of identifying thymic epithelial cellular subpopulations and, more particularly, thymic epithelial progenitors by screening for the co-expression of markers including MHC Class II, UEA1 and Ly51. This method is useful in a range of applications including, but not limited to, assessing or monitoring for the presence of thymic epithelial cell populations and/or facilitating the isolation of or enrichment for these cellular populations of use in a range of research and clinical applications. | 07-15-2010 |
20100184217 | STEM CELL LINES, THEIR APPLICATION AND CULTURE METHODS - New lines of stem cells from the growing antlers of deer (Cervidae) and the application of said cells in the reconstruction of connective tissue, preferentially bone, cartilage or adipose tissue, in humans and animals; as well as a method of culturing them and the application of tissues from growing deer antlers in the production of the MIC-1 stable stem cell line. | 07-22-2010 |
20100184218 | Isolation and Culture-Expansion Methods of Mesenchymal Stem/Progenitor Cells From Umbilical Cord Blood, And Differentiation Method of Umbilical Cord Blood-Derived Meschymal Stem/Progenitor Cells Into Various Mesenchymal Tissues - There is provided a method for the isolation and cultivation of mesenchymal stem/progenitor cells from umbilical cord blood, and also to a method for the differentiation of the umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues. The method include overlaying umbilical cord blood onto Ficoll-Hypaque solution; centrifuging the umbilical cord blood on the Ficoll-Hypaque solution to obtain a mononuclear cell layer; reacting cells obtained by monolayer culture of the mononuclear cells with antibodies to mesenchymal stem/progenitor cell-specific antigens for a predetermined period of incubation time; isolating only cells bound to their corresponding antibodies using a cell sorter; and cultivating the isolated cells, thereby obtaining mesenchymal stem/progenitor cells with high purity and excellent viability. | 07-22-2010 |
20100190250 | Methods of Rejuvenating Cells In Vitro and In Vivo - The present invention provides methods for rejuvenating cells, tissues and the whole body. Also provided are rejuvenating buffers and agents as well as kits for rejuvenating cells. Also provided are methods for dedifferentiating somatic cells and differentiating the cells into other cell types. | 07-29-2010 |
20100190251 | Method for the differentiation of human adult stem cells into insulin-secreting cells - Disclosed is a method for differentiating human adult stem cells into insulin-secreting cells. Human adult stem cells, isolated from the subcutaneous adipose tissues around the eyes, are induced to differentiate into insulin-secreting cells in a medium in the presence of cytokines and growth factors including B27 supplement, fibroblast growth factor-2, epidermal growth factor, nicotinamide, glucagon-like peptide-1, activin A, insulin-like growth factor, betacellulin, etc., with a glucose shift from a high concentration to a low concentration. Having the ability to producing insulin and C-peptide at high levels, the insulin-secreting cells can be excellent curatives for type 1 diabetes mellitus. | 07-29-2010 |
20100197019 | Method for Cultivation of Hair Follicular Dermal Sheath Cells - A method for culture of hair follicular dermal sheath cells or precursor cells thereof which are potent cellular materials for such as hair regeneration by cell transplantation is provided. That is, by performing culture in an animal cell culture medium supplemented with platelet-derived growth factor AA (PDGF-AA) and fibroblast growth factor 2 (FGF2), hair follicular dermal sheath cells are proliferated while sustaining their function, or hair follicular dermal sheath precursor cells are differentiated into dermal sheath cells and proliferated. | 08-05-2010 |
20100221833 | PROCESS FOR DIFFERENTIATION OF VASCULAR ENDOTHELIAL PROGENITOR CELLS FROM EMBRYOID BODIES DERIVED FROM EMBRYONIC STEM CELLS USING HYPOXIC MEDIA CONDITION - The present invention provides a process for differentiation of vascular endothelial progenitor cells from embryoid bodies derived from embryonic stem cells, the process comprising: (a) treating a culture medium comprising embryoid bodies derived from embryonic stem cells such that the concentration of oxygen dissolved in the culture medium is in the range of about 1 ppm to about 5 ppm; (b) culturing the culture medium prepared in step (a) in an incubator in which the oxygen (O | 09-02-2010 |
20100227404 | WATER EXTRACT OF ANTRODIA CAMPHORATA FOR IMMUNOSTIMULATORY EFFECT AND PREPARATION METHOD THEREOF - A preparation method for a water extract of the fruiting body of | 09-09-2010 |
20100240130 | Differentiation and Enrichment of Islet-like Cells from Human Pluripotent Stem Cells - Methods for differentiating human pluripotent stem cells into islet-like cells are provided. In certain embodiments, the methods utilize sequential culturing of the human pluripotent stem cells with certain factors to produce islet-like cells. In certain embodiments, the population of cells produced by the methods is further enriched for islet-like cells. | 09-23-2010 |
20100248366 | Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes - Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence. | 09-30-2010 |
20100261276 | Method For The Preparation Of Dermal Papilla Tissue Employing Mesenchymal Stem Cells - A method for the preparation of dermal papilla tissue comprising the step of culturing mesenchymal stem cells in a medium having a specific composition is provided. The method makes it possible to form in vitro a quantity of dermal papilla tissues having hair follicle inducting ability and, accordingly, it can be effectively used for the treatment of alopecia through cell transplantation. | 10-14-2010 |
20100261277 | METHODS AND COMPOSITIONS FOR ENHANCED DIFFERENTIATION FROM EMBRYONIC STEM CELLS - The invention provides methods for differentiating pluripotent stem cells such as ES cells with improved progenitor and differentiated cell yield using low oxygen conditions and optionally in the absence of exogenously added differentiation factors. | 10-14-2010 |
20100267141 | CHEMICAL APPROACHES FOR GENERATION OF INDUCED PLURIPOTENT STEM CELLS - The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency. | 10-21-2010 |
20100273259 | SUBSTRATES AND METHODS FOR CULTURING STEM CELLS - The present disclosure provides a device and a cell culture system comprising a substrate that generates significant chemical ion signatures adapted for culturing stem cells. This disclosure further provides unique surface properties, such as surface wettability, along with defined polymer microspot environments in an array, for effectively supporting the propagation and differentiation of human pluripotent stem cells in vitro. Methods of culturing, maintenance, differentiating stem cells as well as reprogramming somatic cells into stem cells using the device and the cell culture system with the suitable substrates, along with suitable culture media, are also provided. | 10-28-2010 |
20100285584 | ENGINEERED CELLULAR PATHWAYS FOR PROGRAMMED AUTOREGULATION OF DIFFERENTIATION - The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered. | 11-11-2010 |
20100285585 | METHOD FOR EXTRACELLULAR MATRIX MEDIATED DIFFERENTIATION AND PROLIFERATION OF STEM CELLS - There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells | 11-11-2010 |
20100285586 | METHOD OF PRODUCING A POPULATION OF CELLS - A method is provided for producing an in vitro or ex vivo method of producing a population of lineage committed haematopoietic progenitor, or mature haematopoietic cells, other than cells of the neutrophil lineage, which method comprises the steps of: (a) providing a population of cells comprising haematopoietic progenitor cells; and (b) culturing the population of cells in an animal cell culture medium comprising one or more cytokines that differentiate said progenitor cells into lineage committed haematopoietic progenitor and/or mature haematopoietic cells, under conditions of low oxidative stress, the culture medium being agitated when the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and the progeny thereof under static conditions, to produce a population of lineage committed haematopoietic progenitor, or mature haematopoietic cells, other than cells of the neutrophil lineage. The resulting population of cells can be used to increase the number of haematopoietic cells in a patient. | 11-11-2010 |
20100297763 | CONDITIONALLY IMMORTALIZED LONG-TERM STEM CELLS AND METHODS OF MAKING AND USING SUCH CELLS - Disclosed are methods for conditionally immortalizing stem cells, including adult and embryonic stem cells, the cells produced by such methods, therapeutic and laboratory or research methods of using such cells, and methods to identify compounds related to cell differentiation and development or to treat diseases, using such cells. A mouse model of acute myeloid leukemia (AML) and cells and methods related to such mouse model are also described. | 11-25-2010 |
20100297764 | USE OF A HYDROGEL FOR THE CULTURE OF CHONDROCYTES - The invention relates to the use of a silanised hydroxypropylmethylcellulose (HPMC) or silanised hydroxyethylcellulose (HEC) hydrogel, self-crosslinking as a function of pH, for the three-dimensional ex vivo culture of chondrocytes. | 11-25-2010 |
20100297765 | METHOD FOR PRODUCING LANGERHANS CELLS OR INTERSTITIAL DENDRITIC CELLS OR BOTH FROM CD14+ MONOCYTES - The present invention relates to a method for preparing Langerhans cells or interstitial dendritic cells or both from CD14 | 11-25-2010 |
20100304486 | METHODS OF GENERATING EMBRYOID BODIES AND USES OF SAME - Methods of generating embryoid bodies (EBs) by culturing embryonic stem cells (ESCs) under static conditions followed by culturing the cells under dynamic conditions using e.g., a Glass Bulb-shaped Impeller (GBI) or shaking a culture vessel are provided. Also provided are methods of generating expanded and/or differentiated cells from the EBs of the invention and methods of using same for treating disorders requiring cell replacement therapy. | 12-02-2010 |
20100323443 | Composition and method for enabling proliferation of pluripotent stem cells - The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency. | 12-23-2010 |
20100323444 | CULTURING AND DIFFERENTIATING NEURAL PRECURSOR CELLS - Systems and methods have been developed for large-scale propagation and differentiation of populations of neurons and glia from neural precursor cells derived from postnatal brain. Under culture conditions containing pituitary extract and mitogenic factors, cells derived from neural stem cells can be attached to a substrate, maintained and serially passaged in culture. Upon removal of mitogenic factors, clusters of neural progenitor cells can be induced that co-express markers of neural stem cells and immature neurons. Unlimited numbers of cells at characterized stages of neurogenesis can be produced. Upon maturation, neuronal cells extend processes and differentiate into mature neuronal phenotypes capable of generating action potentials. | 12-23-2010 |
20100330672 | HUMAN STEM CELLS ORIGINATING FROM HUMAN AMNIOTIC MESENCHYMAL CELL LAYER - Neural stem cells which can be provided stably and which are free from the problem of compatibility in transplantation are disclosed. The stem cells are separated from human amniotic mesenchymal cell layer and express vimentin, nestin and BrdU which are markers of neural stem cells. The stem cells can also be differentiated to cells expressing alkaline phosphatase, that is, osteocytes, and to cells expressing collagen type II, that is, chondrocytes. | 12-30-2010 |
20110003387 | METHOD OF PRODUCING ERYTHROCYTES WITHOUT FEEDER CELLS - Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place. | 01-06-2011 |
20110008892 | METHODS OF TISSUE GENERATION AND TISSUE ENGINEERED COMPOSITIONS - Provided are methods and compositions for constructing stable mammalian embryonic epithelial tissues and organs as well as constructing kidney tissue, and treating renal failure. Disclosed are methods of using an active epithelial growth factor having the capability of effectuating induction of growth and morphogenesis is cells. | 01-13-2011 |
20110014702 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells capable of producing insulin following transplantation into an animal. | 01-20-2011 |
20110014703 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into cells expressing markers characteristic of the pancreatic endocrine lineage that co-express PDX1, NKX6.1, but do not express CDX2 and NGN3. | 01-20-2011 |
20110014704 | ISOLATION OF MULTI-LINEAGE STEM CELLS - The present application discloses a method of manipulating a biological sample of cells, which includes multi-lineage stem cells, progenitor cells, other marrow stromal cells: allowing the sample of cells to settle in a container; transferring supernatant from the container to another container; and isolating cells from the supernatant, which has comparatively lower density in the sample. | 01-20-2011 |
20110020932 | IN VITRO DIFFERENTIATION/INDUCTION OF LYMPHOCYTE FROM STEM CELL HAVING GENOTYPE PROVIDED AFTER GENE RECONSTITUTION - The present invention provides a production method of a functional differentiated cell having a post-rearrangement genotype of a particular antigen receptor gene, which includes culturing a stem cell having the genotype in a medium to give the differentiated cell derived from the stem cell. As the stem cell having the genotype, a stem cell (e.g., ES cell) established by transplantation of the nucleus of a cell having the genotype is preferable. As the differentiated cell, NKT cell is preferable. | 01-27-2011 |
20110027886 | METHOD OF INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO HEMANGIOBLAST - The present invention relates to a composition for inducing embryonic stem cell differentiation comprising a MEK/ERK (mitogen-activated protein kinase kinase/extracellular regulated kinase) signal transduction inhibitor and BMP (bone morphogenetic protein), and a method for inducing differentiation of embryonic stem cells into mesodermal cells using the same. Further, the mesodermal cells obtained by the above method are able to differentiate into various mesenchymal tissue cells. In particular, the present invention relates to a method for inducing differentiation into hemangioblast by culturing the mesodermal cells obtained by the above method in the presence of VEGF (vascular endothelial cell growth factor) and bFGF (basic fibroblast growth factor). The differentiated hemangioblasts can be further differentiated into vascular endothelial cells, vascular smooth muscle cells, and hematopoietic stem cells under various culture conditions. | 02-03-2011 |
20110033931 | DE-DIFFERENTIATION OF HUMAN CELLS - Methods of de-differentiating somatic cells to an embryonic stem cell state comprising direct delivery of a protein into the somatic cell, wherein the protein effects de-differentiation of the somatic cell to an embryonic stem cell phenotype. | 02-10-2011 |
20110059526 | REPROGRAMMING A CELL BY INDUCING A PLURIPOTENT GENE THROUGH USE OF AN HDAC MODULATOR - The invention relate to methods, compositions, and kits for reprogramming a cell. In one embodiment, the invention relates to a method comprising inducing the expression of at least one gene that contributes to a cell being pluripotent or multipotent. In yet another embodiment, the method comprises inhibiting the activity of an HDAC with an HDAC inhibitor and inducing the expression of at least one gene that contributes to a cell being pluripotent or multipotent. In still another embodiment, the invention relates to a method for reprogramming comprising exposing a cell to more than one agent to inhibit more than ore type of regulatory protein. In yet another embodiment, the invention relates to a reprogrammed cell or an enriched population of reprogrammed cells that can have characteristics of an ES-like cell, which can be re- or trans-differentiated into various differentiated cell types | 03-10-2011 |
20110065190 | BLOOD PLATELET INDUCTION METHOD - A novel induction method that effectively produces platelets from platelet precursor cells (e.g., hemopoietic stem/precursor cells) is disclosed. The method includes culturing platelet precursor cells in a culture solution in which a composite membrane is immersed so that the platelet precursor cells differentiate into platelets, the composite membrane including a porous support membrane and a porous thin membrane, the porous thin membrane being stacked on at least one side of the porous support membrane. | 03-17-2011 |
20110070645 | COMPOSITIONS AND METHODS FOR PROMOTING THE GENERATION OF PDX1+ PANCREATIC CELLS - Certain embodiments disclosed herein are directed to a method of producing pancreatic cells or pancreatic cell precursors by exposing human embryonic stem cells to an effective amount of at least one compound listed in Table I to differentiate the human embryonic stem cells into the pancreatic cells or the pancreatic cell precursors. Kits and pancreatic cell lines produced using the methods are also described. | 03-24-2011 |
20110070646 | Biomimetic Cell Scaffolds - This invention relates to biomimetic implants containing mammalian cells which are induced by their environment to produce gradients of diffusible factors. This may be useful in eliciting a physiological pro-angiogenic response which stimulates angiogenesis and revascularisation in tissue adjacent the implant, for example in therapeutic applications in which increased angiogenesis and/or vasculogenesis is required. This may also be useful in providing biomimetic mammalian cell niches for cell culture and differentiation. | 03-24-2011 |
20110091971 | Differentiation of Pluripotent Stem Cells - The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. The present invention also provides methods to generate and purify agents capable of differentiating pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. | 04-21-2011 |
20110097799 | CARDIOMYOCYTE PRODUCTION - Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided. | 04-28-2011 |
20110111502 | ELIMINATION OF IMMUNE RESPONSES TO VIRAL VECTORS - The present invention relates to the use of immunogenic peptides comprising a T-cell epitope derived from a viral vector antigen and a redox motif such as C-(X)2-[CST] or [CST]-(X)2-C in the prevention and/or suppression of immune responses to viral vectors and in the manufacture of medicaments therefore. | 05-12-2011 |
20110129922 | METHOD FOR INDUCING DIFFERENTIATION INTO CARDIOMYOCYTES USING G-CSF - A method for inducing differentiation of ES cells into cardiomyocytes, which comprises contacting the ES cells with an agonist for G-CSF receptor. | 06-02-2011 |
20110143436 | COMPOSITIONS AND METHODS FOR REPROGRAMMING EUKARYOTIC CELLS - The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells. | 06-16-2011 |
20110143437 | METHOD FOR USING DIRECTING CELLS FOR SPECIFIC STEM/PROGENITOR CELL ACTIVATION AND DIFFERENTIATION - A method is provided, including obtaining a population of antigen-presenting cells, enriching a population of stem/progenitor cells within a larger population of cells, activating the population of antigen-presenting cells and, following the activating, inducing at least one process selected from the group consisting of: differentiation, expansion, activation, secretion of a molecule, and expression of a marker, by exposing the enriched stem/progenitor cell population to the population of antigen-presenting cells. Other applications are also described. | 06-16-2011 |
20110151560 | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon. | 06-23-2011 |
20110151561 | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to increase the expression of NGN3 and NKX6.1 in populations of cells expressing markers characteristic of the pancreatic endocrine lineage. | 06-23-2011 |
20110151562 | WHITE BLOOD CELL DIFFERENTIATION REAGENT AND METHOD - A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H | 06-23-2011 |
20110177597 | CELLULAR DIFFERENTIATION PROCESS AND ITS USE FOR BLOOD VESSEL BUILD-UP - A process of differentiation of stem cells through the use of specific oxygen concentrations, provided that the stem cells are not human embryonic stem cells, and seeded on a support, in an appropriate culture medium, wherein the differentiation leads to:
| 07-21-2011 |
20110201113 | Ex Vivo Progenitor and Stem Cell Expansion and Differentiation for Use in the Treatment of Disease of the Nervous System - The present invention relates to a simplified process, which is shorter in time, for propagation of proliferating cells, such as e.g. progenitor or stem cells, by means of a biphasic culturing system having a differentiation supporting component and a proliferation supporting component, and to the use of the stem cell cultures obtained in this way for cell therapy purposes. The present invention invention describes a method, which is highly efficient to prime stem or progenitor cells to differentiation using non-attachment matrices and differentiation supporting component. The cells produced therefrom may be used to treat a variety of neurodegenerative disorders, | 08-18-2011 |
20110201114 | Manufacturing Method of Immune Killer Cells - The present invention relates to a manufacturing method of immune killer cells characterized in that an immune killer cell is induced in a culture medium containing concanavalin A (ConA), and the immune killer cell is maintained or expanded in a culture procedure. Antibody proteins are not used as a stimulant in the culture process to avoid the risk of being infected by zoonotic diseases and effectively increase the number and specific release of the immune killer cells. | 08-18-2011 |
20110212523 | DIFFERENTIATION-INDUCING CULTURE MEDIUM ADDITIVE AND USE THEREOF - Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid. | 09-01-2011 |
20110250686 | NOVEL HEPATOCYTE-LIKE CELLS AND HEPATOBLAST-LIKE CELLS DERIVED FROM hBS CELLS - The present invention relates to a novel hepatocyte-like cell population derived from hBS cells and to the potential use of such heopatocyte-like cells in e.g. medical treatment, drug screening and toxicity testing. Furthermore, the invention relates to 5 hepatoblast-like cells that may have suitable characteristics so that they can be used for the same applications as the hepatocyte-like cells and that furthermore may be used in in vitro studies of hepatogenesis such as early hepatogenesis or hepatoregenerative disorders. Both the hepatocyte-like and the hepatoblast-like cells according to the invention express drug transporter and/or drug metabolising 10 characteristics either at the gene or protein expression level. | 10-13-2011 |
20110263017 | TECHNIQUE FOR REGULATING REGENRATION OF TISSUE OR FAULTY OR ABNORMAL PART IN ORGAN USING NELL-1 - The object aims to form and maintain a cell, a tissue or an organ induced by differentiation. Disclosed is a composition for inducing the differentiation of a cell capable of being differentiated in a given direction to thereby produce a cell, a tissue or an organ through the further induction of the differentiation in the given direction. The composition comprises NELL-1 or a substance which can be altered so as to act as NELL-1 upon the differentiation. Also disclosed is a composition for maintaining a cell, a tissue or an organ produced by the induction of the differentiation. | 10-27-2011 |
20110263018 | CORE-SHELL STRUCTURED DELIVERY SYSTEM FOR GROWTH FACTORS, A PREPARATION METHOD THEREOF, AND USE THEREOF FOR THE DIFFERENTIATION OR PROLIFERATION OF CELLS - The present invention relates to a method of preparing a delivery system capable of loading bioactive growth factors that are essential for the differentiation and proliferation of cells and is characterized as loading at least two types of components comprising growth factors in a single carrier, whereby the release of each of the plurality of growth factors can be temporally controlled. Specifically, the method of preparing a microcapsule type growth factor delivery system according to the present invention includes: (1) preparing a polymeric microsphere comprising a first component, and then encapsulating the microspheres by electrodropping the polymer microsphere into another polymer comprising a second component, thereby manufacturing a core-shell structured, microcapsule type delivery system, or (2) encapsulating a polymer solution comprising a first component by electrodropping the polymer solution into another polymer comprising a second component, thereby manufacturing a core-shell structured microcapsule type delivery system. The present invention also provides a stem cell differentiation method involving bringing a microcapsule type delivery system loaded with multiple growth factors according to the present invention into contact with stem cells. | 10-27-2011 |
20110269231 | PROTEOGLYCAN-BINDING PEPTIDES THAT MODULATE STEM CELL BEHAVIOR - Biomaterials presenting synthetic peptides and methods of preparing biomaterials are disclosed. More particularly, the disclosure is directed to biomaterials including a substrate including a synthetic peptide thereon, wherein the synthetic peptide is selected from synthetic proteoglycan-binding peptides, synthetic glycosaminoglycan-binding peptides, and combinations thereof. The present disclosure is further directed to methods of preparing the biomaterials for use in sequestering endogenous proteoglycans and endogenous glycosaminoglycans, increasing stem cell proliferation, reducing spontaneous stem cell differentiation, and enhancing induced osteogenic differentiation of mesenchymal stem cells. | 11-03-2011 |
20110281355 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells expressing markers characteristic of the pancreatic endoderm lineage, wherein greater than 50% of the cells in the population co-express PDX1 and NKX6.1. | 11-17-2011 |
20110281356 | COMPOUNDS FOR STEM CELL DIFFERENTIATION - Methods and small molecule compounds for stem cell differentiation are provided. One example of a class of compounds that may be used is represented by the compound of Formula I: | 11-17-2011 |
20110281357 | Methods to cause differentiation of T-cells for use in cell therapy - A method resulting in differentiation of T-cells for use in cell therapy includes labeling the T-cells with a first array of antibodies specific for T-cell surface antigens; applying a universal cross-linking agent to the labeled T-cells with the first array of antibodies; labeling the T-cells with a second array of antibodies specific for T-cell surface antigens; and applying a universal cross-linking agent to the labeled T-cells with the second array of antibodies. | 11-17-2011 |
20110287538 | METHOD FOR PRODUCTION OF REPROGRAMMED CELL USING CHROMOSOMALLY UNINTEGRATED VIRUS VECTOR - An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments. | 11-24-2011 |
20110287539 | Optimized Methods for Differentiation of Cells into Cells With Hepatocyte and Hepatocyte Progenitor Phenotypes, Cells Produced by the Methods, and Methods of Using the Cells - The invention is directed to methods for culturing cells so that the cells are induced to differentiate into cells that express hepatocyte phenotypes and hepatocyte progenitor phenotypes. More particularly, the invention relates to methods for culturing cells so that the cells are induced to differentiate into cells that express a definitive endodermal phenotype, a liver-committed endodermal phenotype, a hepatoblast phenotype, and hepatocyte phenotype. The invention is also directed to cells produced by the methods of the invention. The cells are useful, among other things, for treatment of liver deficiency, liver metabolism studies, and liver toxicity studies. | 11-24-2011 |
20110287540 | METHOD FOR CONTROLLING PROLIFERATION OF CORD BLOOD HEMATOPOIETIC STEM CELLS AND USE THEREOF - The present invention provides a method for controlling the proliferation and differentiation of cord blood-derived hematopoietic stem cells with excellent safety when proliferating them by culturing. The hematopoietic stem cells are inoculated into a medium containing a sonicated liquid component of cord blood. The proliferation and differentiation of the cord blood hematopoietic stem cells can be inhibited in the presence of the sonicated liquid component of cord blood. On the contrary, the proliferation of cord blood hematopoietic stem cells can be accelerated by inoculating the hematopoietic stem cells into a medium containing a non-sonicated liquid component of cord blood. Thus, according to the present invention, by using serum derived from cord blood, it is possible to regulate the inhibition of the proliferation and differentiation of cord blood hematopoietic stem cells and the acceleration of the proliferation of the same as desired. | 11-24-2011 |
20110300630 | METHODS FOR TREATING DEGENERATIVE DISEASES/INJURIES - Invented is a method of treating degenerative diseases/injuries, in a mammal, including a human, in need thereof which comprises the administration of a therapeutically effective amount of a non-peptide TPO receptor agonist to such mammal. | 12-08-2011 |
20110306132 | CULTURE MEDIUM AND METHOD FOR INDUCING DIFFERENTIATION INTO BONE CELLS - Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of bone cells under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into bone cells comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into bone cells, a ligand for endothelial cell differentiation gene (Edg) family receptors and selenium, which culture medium is serum-free or contains a low concentration of serum. The method for inducing differentiation from somatic stem cells into bone cells comprises culturing the somatic stem cells that can differentiate into bone cells in the above-described culture medium. | 12-15-2011 |
20110306133 | CULTURE MEDIUM AND METHOD FOR INDUCING DIFFERENTIATION INTO ADIPOCYTES - Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum. | 12-15-2011 |
20120003739 | POPULATIONS OF EXPANDED AND RE-DIFFERENTIATED ADULT ISLET BETA CELLS CAPABLE OF PRODUCING INSULIN AND METHODS OF GENERATING SAME - A population of expanded adult islet beta cells is provided as well as a population of expanded and redifferentiated adult islet beta cells. Methods of generation of the populations of cells are provided. | 01-05-2012 |
20120009681 | PLATFORM OF DENDRITIC CELL (DC)-BASED VACCINATION - The present invention discloses novel dendritic cell maturation-inducing cytokine cocktails, and methods for inducting type-1 polarized dendritic cells in serum-free conditions which enhance the desirable properties of DC1s generated in serum-supplemented cultures. The invention further discloses methods and systems using IFNγ and other ligands of the IFNγ receptor, in combination with IFNα (or other type I interferons), poly I:C, and other IFNα (and IFNβ) inducers to enhance the IL-12-producing properties of dendritic cells. More specifically, the present invention discloses type-1 polarized dendritic cells that have a unique combination of a fully-mature status and an elevated, instead of “exhausted”, ability to produce IL-12p70. allows for the generation of fully-mature DC1s in serum-free AIM-V medium. The invention discloses systems that use the foregoing products and methods to facilitate the clinical application of DC1-based vaccines and the identification of novel factors involved in the induction of Th1 and CTL responses by DC1. | 01-12-2012 |
20120021517 | Bacterial Mediated Delivery of Nuclear Protein Into Pluripotent and Differentiated Cells - A modified | 01-26-2012 |
20120021518 | GROWTH FACTOR HOMOLOG ZVEGF4 - Polypeptide growth factors, methods of making them, polynucleotides encoding them, antibodies to them, and methods of using them are disclosed. The polypeptides comprise an amino acid segment that is at least 70% identical to residues 52-179 of SEQ ID NO:2 or residues 258-370 of SEQ ID NO:2. Multimers of the polypeptides are also disclosed. The polypeptides, multimeric proteins, and polynucleotides can be used in the study and regulation of cell and tissue development, as components of cell culture media, and as diagnostic agents. | 01-26-2012 |
20120021519 | EFFICIENT INDUCTION OF PLURIPOTENT STEM CELLS USING SMALL MOLECULE COMPOUNDS - The disclosure features a method of producing a reprogrammed cell (e.g. an induced pluripotent stem cell or an undifferentiated cell) from a differentiated (e.g. somatic) cell. In some embodiments, the methods includes contacting a differentiated (e.g. somatic cell) with a TGFBR1 inhibitor or anti-TGF-β-antibody to produce a reprogrammed cell (e.g. pluripotent stem cell or undifferentiated cell). Embodiments of the present invention relate to a reprogrammed cell and methods and compositions for producing a chemically produced reprogrammed cell or populations thereof. | 01-26-2012 |
20120028354 | SYSTEMS AND METHODS FOR MAKING HEPATOCYTES FROM EXTRAHEPATIC SOMATIC STEM CELLS AND USE THEREOF - A method for preparing isolated hepatocytes is disclosed. The method comprises: a) culturing mesenchymal stem cells (MSCs) in a medium comprising hepatic growth factor (HGF) to cause the MSCs to differentiate toward hepatocytes, wherein the MSCs are isolated from bone marrow or umbilical cord blood; b) culturing cells from a) in a medium comprising HGF and oncostatin M (OSM) to facilitate the cell differentiation toward hepatocytes; and c) culturing cells from b) in a medium comprising OSM to cause the differentiated cells to mature into hepatocytes, and thereby producing the isolated hepatocyte cells. | 02-02-2012 |
20120034694 | CELL CULTURE SUPPORT AND CELL CULTURE METHOD - The present invention relates to a cell culture support for culturing mesenchymal stem cells, which includes en upper surface including a plurality of wells, in which the upper surface has a root mean square roughness Rq of 100 to 280 nm and a linear density of 1.6 to 10 per 1 μm length. | 02-09-2012 |
20120045831 | POLY(LACTIC-GLYCOLIC)ACID CROSS LINKED ALENDRONATE (PLGA-ALN) A SHORT TERM CONTROLLED RELEASE SYSTEM FOR STEM CELL DIFFERENTIATION AND DRUG DELIVERY - The present invention relates to a bisphosphonates and synthetic polymeric carriers to form a sustained release system. The present invention also provides a method for preparing a sustained release system, comprising activation of the synthetic polymeric carriers and the cross linking reaction. The present invention further provides methods for enhancing stem cell differentiation into osteogenic lineage and chondrogenic lineage, which comprise culturing a population of stem cells in specific micro-environments. | 02-23-2012 |
20120045832 | MULTIPOTENT NEURAL STEM CELLS - An isolated multipotent neural stem cells has a phenotype identified by expression of the protein β-tubulin IV and Olig2 and the absence of the proteins NG2, PLP, and GFAP. | 02-23-2012 |
20120064627 | APPARATUS AND METHOD FOR CULTURING STEM CELLS - A method is provided to prepare a plurality of microwells suitable for the formation of embryoid bodies from embryonic stem cells. The method requires applying an image-forming material to a heat sensitive thermoplastic material in a designed pattern and heating the material under conditions that reduce the size of the receptive material by at least about 60% to create a mold. A polymer such as PDMS is then applied to the mold and removed to form the microwells. In an alternative aspect, the plurality of microwells on receptive material are prepared by etching a microwell designed pattern into a heat sensitive thermoplastic material support and then heating the material under conditions that reduce the size of the receptive material by at least about 60%. | 03-15-2012 |
20120070897 | Method For Enucleating Nucleated Erythrocyte, And Enucleation Inducer - Provided is a factor capable of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time. More particularly, provided are a method of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time by adding a compound derived from proopiomelanocortin (POMC) to an undifferentiated (nucleated) erythrocyte, and an enucleation inducer including the compound. | 03-22-2012 |
20120077271 | DIFFERENTIATION OF MULTI-LINEAGE PROGENITOR CELLS TO CHONDROCYTES - Fetal blood multi-lineage progenitor cells that are capable of a wide spectrum of transdifferentiation are described, as well as methods of differentiating the progenitor cells into chondrocytes. | 03-29-2012 |
20120088302 | Cell Culture - A method for determining the effect of a plurality of culture conditions on a cell, comprising the steps of a) providing a first set of groups of cell units each comprising one or more cells, and exposing said groups to desired culture conditions; (b) pooling two or more of said groups to form at least one second pool; (c) subdividing the second pool to create a further set of groups of cell units; (d) exposing said further groups to desired culture conditions; (e) optionally, repeating steps (b)-(d) iteratively as required; and (f) optionally assessing the effect on a given cell unit of the culture conditions to which it has been exposed. | 04-12-2012 |
20120094384 | USE OF COMPOUNDS FOR DIFFERENTIATION OF CELLS - The present invention provides a method for differentiating an undifferentiated cell, said method comprising contacting an undifferentiated cell with certain compounds of Formula (I) or a salt thereof. | 04-19-2012 |
20120100610 | REVASCULARIZATION CELLS DERIVED FROM MONONUCLEAR CELLS, AND METHOD OF INDUCING DIFFERENTIATION THEREOF - The present invention relates to a method of safely and simply inducing differentiation of mononuclear cells into cells that promote neovascular stabilization and maturation, and lead to recovering from ischemia or tissue repair. The cells according to the present invention are obtained by inducing differentiation of a mononuclear cell by culturing the mononuclear cell in a medium (particularly a serum-free medium) containing one or more selected from vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), thrombopoietin (TPO), granulocyte-colony stimulating factor (G-CSF) and FMS-like tyrosine kinase 3 ligand (FLT3L), and collecting a cell population expressing CD11 | 04-26-2012 |
20120107934 | Human Late Stage Motor Neuron Progenitor Cells and Methods of Making and Using Same - Motor neuron progenitor (MNP) cells and populations of MNP cells, are provided, in particular, populations of human late stage MNP cells having a purity of greater than about 65% late stage MNP cells and high-purity populations of MNP cells having greater than 95% viable cells, as well as method of making and using the same, including deriving late stage MNP cells from pluripotent embryonic stem cells, producing high-purity populations of late stage MNP cells, producing populations of viable MNP cells, transporting viable MNP cells, and transplanting MNP cells. | 05-03-2012 |
20120115229 | EMBRYONIC STEM CELLS AND NEURAL PROGENITOR CELLS DERIVED THEREFROM - The present invention provides undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, and committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. The invention also provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and the cells that are generated along the pathway of neural differentiation may be used for the study of the cellular and molecular biology of human neural development, for the discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, for drug discovery and for the development of screening assays for teratogenic, toxic and neuroprotective effects. | 05-10-2012 |
20120122218 | Harnessing Cell Dynamics to Engineer Materials - The invention features synthetic materials and methods for inducing cell behavior. Matrix materials induce cell differentiation and cell manipulation based on mechanical and physical characteristics of the materials rather than chemical characteristics. | 05-17-2012 |
20120129256 | METHOD OF PREPARING PLURIPOTENT STEM CELLS - The invention relates to biotechnology, and particularly to the preparation of pluripotent stem cells. The method involves introduction into umbilical cord and placental stem cells of RNA with at least one sequence which ensures the transition of cells to the pluripotent state. The method enables to effectively prepare pluripotent stem cells from the cells of mammalian placenta and umbilical cord which have not yet acquired somatic mutations, which reduces the risk of oncogenesis and other adverse effects of reprogramming. | 05-24-2012 |
20120142102 | ISOLATION OF HUMAN UMBILICAL CORD BLOOD-DERIVED MESENCHYMAL STEM CELLS - Human umbilical cord blood (UCB) contains mesenchymal stem cells (MSCs) that have higher multipotentiality than adult marrow-derived MSCs. However, it has been difficult to obtain these cells because the frequency of MSCs in UCB is extremely rare (0.4-30 out of 1×10 | 06-07-2012 |
20120142103 | METHOD FOR INDUCING DIFFERENTIATION INTO EPITHELIAL PROGENITOR CELL/STEM CELL POPULATION AND CORNEAL EPITHELIAL CELL POPULATION FROM INDUCED PLURIPOTENT STEM CELLS - The present invention relates to: a method for inducing differentiation into an epithelial progenitor cell/stem cell population or a corneal epithelial cell population by culturing, under particular conditions, induced pluripotent stem cells induced from mammalian somatic cells or undifferentiated stem cells; an epithelial progenitor cell/stem cell population or a corneal epithelial cell population obtained by the method; and a cell preparation for the treatment of epithelial disease and a cell sheet, which are prepared using these cell populations. | 06-07-2012 |
20120149110 | PROCESS FOR PRODUCTION OF BIOARTIFICIAL ORGAN - A method for inducing the differentiation of stem cells into tissue cells; a bioartificial organ, which contains the tissue cells; and a material for medical purposes, which contains the bioartificial organ are disclosed. Stem cells capable of proliferation, self-replication and differentiation are used and cultured by a hanging-drop method to three-dimensionally construct a cell mass of embryoid bodies, and the cell mass is cultured in the presence of HGF, GDNF, b-FGF, BMP7 and EGF. In this manner, the stem cells can be differentiated into tissue cells. A bioartificial organ can be produced using cells that have been differentiated into the tissue cells. Further, a material for medical purposes can be provided. | 06-14-2012 |
20120156778 | METHOD FOR EFFICIENT PRODUCTION OF INDUCED PLURIPOTENT STEM CELLS UTILIZING CELLS DERIVED FROM ORAL MUCOSA - A main object of the present invention is to provide a technique to produce iPS cells with less burden on the patient and with high establishment efficiency. iPS cells can be efficiently produced with significantly improved establishment efficiency by selecting cells derived from oral mucosa and introducing, into the cells, reprogramming factors capable of inducing the cells into pluripotent stem cells. | 06-21-2012 |
20120164731 | METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO SKELETAL MUSCLE PROGENITOR CELLS - Provided is a method of producing a skeletal muscle progenitor cell using a pluripotent stem cell, particularly an iPS cell, the method comprising the step 1) of culturing a pluripotent stem cell under serum-free conditions, and in the presence of Activin A, to allow the cell to differentiate into a PDGFRα-positive mesodermal cell, and the step 2) of culturing the mesodermal cell under serum-free conditions, and in the presence of a Wnt signal inducer, to allow the cell to differentiate into a skeletal muscle progenitor cell. Also provided are a cell population containing a skeletal muscle progenitor cell as obtained by the method, and a skeletal muscle regeneration promoting agent and therapeutic agent for muscular diseases such as muscular dystrophy, the promoting agent or agent comprising the skeletal muscle progenitor cell as an active ingredient. | 06-28-2012 |
20120190111 | Differentiation of Pluripotent Stem Cells - The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. | 07-26-2012 |
20120190112 | Differentiation of Pluripotent Stem Cells - The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. | 07-26-2012 |
20120196365 | Differentiation of Pluripotent Stem Cells - The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage. | 08-02-2012 |
20120214236 | GENERATING INDUCED PLURIPOTENT STEM CELLS AND PROGENITOR CELLS FROM FIBROBLASTS - The present disclosure provides a method of generating progenitor cells, such as hematopoietic or neural progenitor cells, from fibroblasts, such as dermal fibroblasts, comprising providing fibroblasts that express or are treated with a POU domain containing gene or protein and culturing the cells under conditions that allow production of progenitor cells, without traversing the pluripotent state. Also provided is a method of isolating a subpopulation of fibroblasts with reprogramming potential comprising providing fibroblasts that express an Oct-4-reporter and isolating cells that are positive for the reporter. Further provided is a method of generating reprogrammed fibroblast-derived induced pluripotent stem cells. Also provided are uses and assays of the cells produced by the methods of the disclosure. | 08-23-2012 |
20120238019 | STEM CELL CULTURE MEDIA FOR MAINTAINING STEMNESS OR INDUCING DIFFERENTIATION OF STEM CELLS - The present invention provides a medium for maintaining stemness of stem cells comprising a protein kinase C inhibitor in a basal medium; and a method for culturing stem cells while maintaining stemness thereof, using the same. And also, the present invention provides a medium for inducing differentiation of stem cells comprising a protein kinase C activator in a basal medium; and a method for inducing differentiation of stem cells, using the same. | 09-20-2012 |
20120238020 | Megakaryocyte and Platelet Production from Stem Cells - Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein. | 09-20-2012 |
20120244620 | COMPOSITIONS AND METHODS FOR INDUCING THE ACTIVATION OF IMMATURE MONOCYTIC DENDRITIC CELLS - The present invention provides methods for inducing the maturation of immature dendritic cells (DC) and for activating those cells without the use of a dendritic cell maturation agent. The activated DC can be used for inducing an antigen specific T cell response. Methods of the invention can also comprise the addition of a directional maturation agent, such as interferon gamma, to induce a Th-1 and/or Th-2 bias in the response obtained. The present invention also provides dendritic cell populations useful for activating and for inducing antigen specific T cells. Similarly, activated antigen specific T cell populations, and methods of making the same are provided. | 09-27-2012 |
20120252120 | DIAZONIUM SALT MODIFICATION OF SILK POLYMER - A method for modifying silk polymer by coupling a chemical moiety to a tyrosine residue of a silk polymer is described herein for the purpose of altering the physical properties of the silk protein. Thus, silk proteins with desired physical properties can be produced by the methods described herein. These methods are particularly useful when the introduction of cells to a mammal is desired, since modifications to the silk protein affect the physical properties and thus the adhesion, metabolic activity and cell morphology of the desired cells. The silk protein can be modified to produce, or modify, a structure that provides an optimal environment for the desired cells. | 10-04-2012 |
20120288936 | METHODS FOR REPROGRAMMING CELLS AND USES THEREOF - Described herein are methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also descried are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs). Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas. | 11-15-2012 |
20120295351 | METHOD FOR CLONING PLURIPOTENT STEM CELLS - Embodied herein are methods of reprogramming somatic cells or tissue stem cells to a more multipotent state or even a pluripotent state, the methods do not involve gene transfer of master transcription factor genes/proteins. The methods are also useful for rapid and efficient cloning of induced pluripotent stem cells after gene transfer of master transcription factor genes/proteins. | 11-22-2012 |
20120301962 | VITRONECTIN-DERIVED CELL CULTURE SUBSTRATE AND USES THEREOF - Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented. | 11-29-2012 |
20120309088 | METHODS AND COMPOSITIONS FOR STEM CELL SELF-RENEWAL - The present invention relates to methods for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population. | 12-06-2012 |
20120315701 | PEPTIDE INHIBITING DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS OR HEMATOPOIETIC PRECURSOR CELLS AND USE OF SAME - The present invention provides a novel peptide that can be effectively used to produce or grow tissue-specific stem cells or tissue-specific progenitor cells in vitro. The peptide of the invention is a peptide having an amino acid sequence consisting of the amino acid residues set forth in SEQ ID NO:1, or an analog thereof. A feature of the peptide of the invention is having at least one of the following effects: (1) an effect of inhibiting differentiation of hematopoietic stem cells or hematopoietic progenitor cells into myeloid cells, (2) an effect of promoting amplification of mesenchymal stem cells, and (3) an effect of inducing hematopoietic stem cells from pluripotent stem cells. | 12-13-2012 |
20120329155 | MEDIUM FOR MAMMALIAN SOMATIC CELLS AND ADDITIVE THEREFOR - Disclosed are a medium for mammalian somatic cells with which mammalian somatic cells can be grown effectively when the mammalian somatic cells are cultured, while reducing the amount of serum to be added to the medium as much as possible or without adding serum thereto, and an additive to constitute the medium. By blending of a ligand for an endothelial cell differentiation gene (Edg) family receptor and a ligand for a serotonin receptor to a medium, somatic cells of mammals can be grown even in cases where the medium does not contain serum at all or contains only a small amount thereof. | 12-27-2012 |
20130005038 | Method of Separating Cells and Separation Apparatus - A method and apparatus for discriminating and separating only specific cells in cell samples is provided. The cell separation operation includes inserting a needle-shaped body, on which a substance that selectively binds to a marker substance within each target cell has been immobilized, into each of the cell samples on a substrate, thereby causing the marker substance within each target cell in the cell samples to bind to the needle-shaped body through the substance that selectively binds to the marker substance, and then, putting up the needle-shaped body. The binding force between the needle-shaped body and each target cell when the marker substance within each target cell has bound to the needle-shaped body is made greater than an adhesion force of each of the cell samples to the substrate, so that only target cells each containing the marker substance are pulled up and separated from the substrate. | 01-03-2013 |
20130005039 | THERMORESPONSIVE SUBSTRATE WITH MICROGELS, METHOD FOR ITS PREPARATION AND CULTURE METHOD FOR BIOLOGICAL CELLS - A substrate ( | 01-03-2013 |
20130011924 | METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO MESODERMAL CELLS - The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species. | 01-10-2013 |
20130017604 | USE OF ADIPOSE TISSUE-DERIVED STROMAL CELLS FOR CHONDROCYTE DIFFERENTIATION AND CARTILAGE REPAIR - Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed. | 01-17-2013 |
20130017605 | ORALLY BIOAVAILABLE STILBENOIDS- COMPOSITIONS AND THERAPEUTIC APPLICATIONS THEREOF - A novel, bioavailable and safe stilbenoid 3,5-dimethoxy-3,4′-dihydroxystilbene represented by SIR#1 with an unexpected enhanced ability to prevent the accumulation of lipids accompanying the terminal differentiation of adipocytes, thereby inhibiting adipogenesis, and nutraceutical and cosmeceutical compositions comprising, dimethoxy-3,4′-dihydroxystilbene useful for anti-obesity and anti-cellulite therapy, are disclosed. Further the enhanced SIRT-1 activation ability of 3,5-dimethoxy-3,4′-dihydroxystilbene represented by STR#1 and 2,3′,5′,6-tetrahydroxy-trans-stilbene represented by STR#II are disclosed. The enhancement of SIRT-1 polypeptide activity of the said compounds is unexpectedly much higher than resveratrol or its natural analog pterostilbene. Sirtuin modulating compositions comprising an orally bioavailable SIRT-1 enhancing compounds (i) 3,5-dimethoxy-3,4′-dihydroxystilbene represented by STR#I and (ii) 2,3′,5′,6-tetrahydroxy-trans-stilbene represented by are also disclosed. An additional embodiment, also discloses the enhanced anti- | 01-17-2013 |
20130029418 | METHODS AND PRODUCTS FOR TRANSFECTION - The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed. | 01-31-2013 |
20130029419 | Blood-Brain Barrier Model - A method of creating a multicellular blood-brain barrier model is disclosed. In one embodiment, the method comprises culturing primary brain microvascular endothelial cells or embryonic stem cell-derived endothelial cells upon a permeable support in the presence of neural progenitor cells. | 01-31-2013 |
20130045534 | Methods of Deriving Differentiated Cells from Stem Cells - The present invention provides a method of generating definitive endoderm, mesoderm, or ectoderm cells. The method includes culturing embryonic stem cells, parthenogenetic cells, or induced pluripotent stem cells in the presence of a demethylation agent, a histone deacetylase inhibitor, or a combination thereof, and thereafter, culturing the stem cells in the absence of the agent or combination of agents, to produce definitive endoderm cells, mesoderm, or ectoderm cells. | 02-21-2013 |
20130065303 | METHOD FOR INDUCING SPHEROID FORMATION OF ADIPOSE-DERIVED STEM CELLS AND TRANS-DIFFERENTIATION INTO NEURAL LINEAGE - The present invention relates to a basal medium for spheroid formation of adipose-derived stem cells, comprising a substrate; and a chitosan film faulted on a surface of the substrate, wherein the chitosan film comprises chitosan with 60-90% degree of deacetylation, and the chitosan film has a surface roughness defined by a height difference, measured between a highest position and a lowest position thereof, of 1-25 nm. In addition, the present invention further provides a method for inducing the spheroid trans-differentiating into neural lineages by using the basal medium of the present invention. | 03-14-2013 |
20130065304 | DIFFERENTIATION PROCESS OF MESENCHYMAL STEM CELLS AND THERAPEUTIC USE THEREOF - Process for inducing differentiation of mesenchymal stamina cells into neuroblasts and/or neurons that envisions the use of a differentiation solution consisting of retinoic acid and ethanol. | 03-14-2013 |
20130071930 | POLYESTERS, METHODS OF MAKING POLYESTERS AND USES THEREFOR - Polyester compositions and functionalized polyester compositions are provided along with methods of making the compositions as well as methods of using the compositions, for example as a tissue engineering bioscaffold and as a drug-delivery vehicle. | 03-21-2013 |
20130071931 | PROCESS FOR HEPATIC DIFFERENTIATION FROM INDUCED HEPATIC STEM CELLS, AND INDUCED HEPATIC PROGENITOR CELLS DIFFERENTIATED THEREBY - A method for hepatic differentiation of a stem cell selected from among embryonic stem cells, induced pluripotent stem cells or induced hepatic stem cells is presented. More specifically, a stem cell selected from among embryonic stem cells, induced pluripotent stem cells or induced hepatic stem cells is cultured for 1 to 4 weeks in the presence of a TGF-β inhibitor, whereby the hepatic differentiation of the stem cell is realized. | 03-21-2013 |
20130071932 | CULTURE METHOD FOR CAUSING DIFFERENTIATION OF PLURIPOTENT MAMMALIAN CELLS - Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber ( | 03-21-2013 |
20130078721 | Cell Culture Medium for the Growth and Differentiation of Cells of the Hematopoietic Lineage - The present invention relates to a cell culture medium for the growth and/or differentiation of cells of the hematopoietic lineage, comprising: —insulin at a concentration of from 1 to 50 μg/ml; —transferrin at a concentration of from 100 μg/ml to 2000 μg/ml; and—plasma or serum at a concentration of from 1% to 30%. | 03-28-2013 |
20130137176 | Biomatrix Scaffolds for Industrial Scale Dispersal - The present invention provides biomatrix scaffolds for industrial scale dispersal. | 05-30-2013 |
20130137177 | PHARMACEUTICAL OR COSMETIC COMPOSITION CONTAINING NICOTINIC ACID ADENINE DINUCLEOTIDE PHOSPHATE OR DERIVATIVE THEREOF - Provided are a method of preparing a zinc oxide nanostructure electrode and a method of preparing a dye-sensitized solar cell using the same. | 05-30-2013 |
20130143322 | METHOD FOR CONTROLLING PROLIFERATION OF CORD BLOOD HEMATOPOIETIC STEM CELLS AND USE THEREOF - The present invention provides a method for controlling the proliferation and differentiation of cord blood-derived hematopoietic stem cells with excellent safety when proliferating them by culturing. The hematopoietic stem cells are inoculated into a medium containing a sonicated liquid component of cord blood. The proliferation and differentiation of the cord blood hematopoietic stem cells can be inhibited in the presence of the sonicated liquid component of cord blood. On the contrary, the proliferation of cord blood hematopoietic stem cells can be accelerated by inoculating the hematopoietic stem cells into a medium containing a non-sonicated liquid component of cord blood. Thus, according to the present invention, by using serum derived from cord blood, it is possible to regulate the inhibition of the proliferation and differentiation of cord blood hematopoietic stem cells and the acceleration of the proliferation of the same as desired. | 06-06-2013 |
20130157365 | INDUCED PLURIPOTENT STEM CELLS FROM HUMAN UMBILICAL CORD TISSUE-DERIVED CELLS - We have disclosed an induced pluripotent stem cell and the method of preparing the induced pluripotent stem cell from a human umbilical cord tissue-derived cell. More particularly, we have disclosed a human umbilical cord tissue-derived iPS cell which may be differentiated into cells of ectoderm, mesoderm, and endoderm lineages. | 06-20-2013 |
20130164847 | USE OF A PROTEIN TYROSINE KINASE INHIBITOR FOR INDUCING THE DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO CARDIOGENIC CELLS - The present invention pertains to methods of using protein tyrosine kinase (PTK) inhibitors for inducing cell differentiation of mesenchymal stem cells into cardiac cells, and a pharmaceutical composition comprising the differentiated cardiac cells thereof to treat heart diseases. The transplantation of mesenchymal stem cells to the heart provides immunological and functional improvements, but does not provide electrical stability. However, the mesenchymal stem cells treated with PTK inhibitors are induced to be differentiated into cardiogenic cells to provide electrical stability as the electromechanical integration with host heart tissue is improved, and it is thus possible to effectively treat cardiac diseases such as cardiac infarction, cardiac insufficiency, arrhythmia and the like. | 06-27-2013 |
20130177985 | DNA Sequence Encoding a Retinoic Acid Regulated Protein - The present invention concerns a novel retinoic acid regulated gene whose expression product displays useful morphogenic/mitogenic properties. The present invention further concerns an isolated nucleic acid of SEQ ID NO:1 encoding a retinoic acid regulated expression product having an amino acid sequence of SEQ ID NO:2. | 07-11-2013 |
20130189785 | GENERATION OF CARDIOMYOCYTES FROM HUMAN PLURIPOTENT STEM CELLS - Methods for generating high-yield, high-purity cardiomyocyte progenitors or cardiomyocytes from pluripotent cells are described. Wnt/β-catenin signaling is first activated in pluripotent cells, e.g., by inhibition of Gsk-3 to obtain a first population of cells. Wnt/β-catenin signaling is then inhibited in the first cell population to induce cardiogenesis under fully defined, growth factor free culture conditions. | 07-25-2013 |
20130196435 | STENCIL PATTERNING METHODS AND APPARATUS FOR GENERATING HIGHLY UNIFORM STEM CELL COLONIES - A method for producing highly uniform cell colonies in a cell culture dish with the use of stencils made from an elastomeric sheet sized to fit within the cell culture dish, having a singular opening or a plurality of openings of a number, pitch and diameter configured to optimally control the geometric growth parameters of a cell colony. The uniform cell colonies are produced by placing the stencil in a cell culture dish and hydropilizing the stencil. The stencil is overlayed with cell culture media and seeded with seed cells that are preferably grown for at least a day before the stencil is removed to produce a pattern of seeded cells with controlled pitch, colony diameter and density within the culture dish that grow to become highly uniform cell colonies. A kit with culture dish, stencil, culture media and growth media is also provided. | 08-01-2013 |
20130196436 | CARDIOMYOCYTE PRODUCTION - Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided. | 08-01-2013 |
20130196437 | CARDIOMYOCYTE PRODUCTION - Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided. | 08-01-2013 |
20130210145 | GENERATION OF INNER EAR CELLS - Methods for generating cells of the inner ear, e.g., hair cells and supporting cells, from stem cells, e.g., mesenchymal stem cells, are provided, as well as compositions including the inner ear cells. Methods for the therapeutic use of the inner ear cells for the treatment of hearing loss are also described. | 08-15-2013 |
20130210146 | Methods and Compositions for Generation of Induced Pluripotent Stem Cells By RNAA - The present disclosure provides methods for inducing somatic cells to form induced pluripotent stem (iPS) cells. The method includes introducing a small activating RNA (saRNA) molecule into the somatic cell, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure also provides compositions and kits comprising a saRNA molecule that increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure provides iPS cells comprising at least one exogenous saRNA molecule, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. | 08-15-2013 |
20130210147 | Bioactive amino acid sequence and use therefrom - Use of the amino acid sequence Har-Gly-Asp (hRGD) as a bioactive sequence in functional peptides to promote cell adhesion, cell growth, and/or cell differentiation, and in the preparation of hydrogels, preferably hydrogels for cell culture. A hydrogel comprising the hRGD sequence, especially a hydrogel wherein the hRGD sequence is part of the hydrogel scaffold. | 08-15-2013 |
20130217125 | CULTURE METHOD - The invention provides methods of culturing cells, particularly maintaining the differentiation potential of a population of cells with differentiation potential. The methods involve contacting cells with an inhibitor of miRNA-181a* and/or incubation in a serum-free medium. A serum-free medium is also provided. Also provided are progeny stem cells, methods of obtaining them, and uses thereof. | 08-22-2013 |
20130230924 | Equine Amniotic Fluid-Derived Multipotent Stem Cells and a Method for Producing the Same - The present invention relates to equine amniotic fluid-derived multipotent stem cells (eAF-MSCs) and a preparation method thereof. More particularly, the present invention relates to equine amniotic fluid-derived multipotent stem cells which exhibit all negative immunological characteristics with respect to the human markers, CD19, CD20, CD28, CD31, CD34, CD38, CD41a, CD62L, CD62P and CD200, and exhibit all positive immunological characteristics with respect to the human markers, CD44, CD90 and CD105, and have the ability to differentiate into ectoderm, mesoderm or endoderm-derived cells. | 09-05-2013 |
20130236969 | Use of Compounds for Inducing Differentiation of Mesenchymal Stem Cells to Chondrocytes - Use of a compound of Formula 1 for inducing differentiation of mesenchymal stem cells to chondrocytes, and a pharmaceutical composition for treating a cartilage disease, which includes chondrocytes in which differentiation from mesenchymal stem cells is induced by the compound of Formula 1, are provided. Differentiation of the mesenchymal stem cells treated with the compound of Formula 1 to chondrocytes is specifically induced, and thus the compound can be used to effectively treat a cartilage disease such as arthritis, cartilage damage, and a cartilage defect. | 09-12-2013 |
20130252335 | METHOD AND CULTURE MEDIUM FOR IMPROVING PLURIPOTENT STEM CELL DIFFERENTIATION INDUCING EFFICIENCY - The purpose of the present invention is to provide a method and a culture medium which are capable of efficiently inducing the differentiation of pluripotent stem cells such as ES cells and iPS cells into desired cells by a simple means. The differentiation of pluripotent stem cells can be induced by culturing mammal-derived pluripotent stem cells in a differentiation culture medium that does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. Also provided is a differentiation-inducing culture medium which does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. | 09-26-2013 |
20130267025 | Partial Region Polypeptide of REIC/Dkk-3 Protein - A polypeptide capable of strongly inducing and activating dendritic-cell-like cells for treating or prevent cancer by immunotherapy, and DNA encoding the polypeptide. The polypeptide is a polypeptide (a) or (b) consisting of a partial region of the REIC/Dkk-3 protein. | 10-10-2013 |
20130267026 | METHODS OF BUILDING A BODY PORTION - An improved method of implanting cells in the body of a patient includes positioning viable cells on a support structure. One or more blood vessels may be connected with the support structure to provide a flow of blood through the support structure. A support structure may be positioned at any desired location in a patient's body. The support structure may be configured to replace an entire organ or a portion of an organ. An organ or portion of an organ may be removed from a body cells and/or other tissue is removed to leave a collagen matrix support structure having a configuration corresponding to the configuration of the organ or portion of an organ. Alternatively, a synthetic support structure may be formed. The synthetic support structure may have a configuration corresponding to a configuration of an entire organ or only a portion of an organ. | 10-10-2013 |
20130273658 | HUMAN ADIPOCYTE-DERIVED BASEMENT MEMBRANE FOR TISSUE ENGINEERING APPLICATIONS - The present disclosure generally relates to tissue engineering. More particularly, the present disclosure relates to the generation of a new biologically active and biocompatible human derived materials useful in tissue engineering applications. | 10-17-2013 |
20130288374 | PROTEIN DELIVERY SYSTEM TO GENERATE INDUCED PLURIPOTENT STEM (iPS) CELLS OR TISSUE-SPECIFIC CELLS - A novel protein delivery system to generate induced pluripotent stem (iPS) cells is described. The delivery system comprises a construct with a receptor binding domain that recognizes a receptor in a somatic cell, a translocation domain that allows the transfer of an inducer into the cytosolic space, and a cargo bearing domain to which the inducer is attached and facilitates transfer of the inducer into the cell. | 10-31-2013 |
20130295672 | STEM CELL CULTURE MEDIA AND METHODS - The present invention provides novel stem culture compositions and methods. | 11-07-2013 |
20130302893 | METHOD FOR INCREASING THE EFFICIENCY OF INDUCING PLURIPOTENT STEM CELLS - The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission. | 11-14-2013 |
20130309769 | Identification Of Novel Cell Surface Markers For Pancreatic Progenitor Cells And Definite Endodermal Cells - Methods of identifying, isolating and qualifying pancreatic progenitor cells and definite endodermal cells. An isolated population of pancreatic progenitor cells, including at least 75% of cells having a TROP-2+ and/or TROP-2+/GPR50+ expression pattern and an isolated population of definite endodermal cells, including at least 50% of cells having a | 11-21-2013 |
20130316454 | Methods for Producing Tissue Scaffold Directing Differentiation of Seeded Cells and Tissue Scaffolds Produced Thereby - Methods for producing a tissue scaffold which direct differentiation of seeded stem cells on the scaffold to a selected cell type and tissue scaffolds produced thereby are provided. | 11-28-2013 |
20130330825 | ATTACHMENT SUBSTRATES FOR DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN CULTURE - In one embodiment, methods of producing a population of differentiated target cells from a population of undifferentiated pluripotent stem cells (PSC) are provided herein. Such methods may include culturing the population of undifferentiated PSCs, such as human embryonic stem cells (hESC), on an attachment matrix which comprises at least two or more laminin isoforms. The two or more laminin isoforms may include a laminin combination of one or more laminin isoforms that support the hESC cells; and one or more laminin isoforms that would support a population of differentiated target cells. The one or more laminins (LN) that support the hESC cells may be selected from LN-511 or LN-521, while the population of differentiated target cells is a population of cardiomyocytes, and the one or more laminins that would support the cardiomyocytes are selected from LN-411, LN-111, LN-421, LN-211, LN-332, or LN-121. | 12-12-2013 |
20130337564 | Treatment of Pluripotent Cells - The present invention is directed to methods to treat pluripotent cells, whereby the pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of GSK-3B enzyme activity. | 12-19-2013 |
20130344599 | DECELLULARIZATION AND RECELLULARIZATION OF SOLID ORGANS - The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ. | 12-26-2013 |
20140017789 | Brown Fat Cell Compositions and Methods - Methods of developing and using cell lines, such as stem cell lines, for therapeutic or cosmetic use. In one embodiment the cell lines are used to treat a wide range of degenerative and metabolic disorders including, but not limited to, obesity, diabetes, hypertension, and cardiac deficiency. Also described are methods of using such cell lines to screen for compounds that play a role in regulating a variety of processes. | 01-16-2014 |
20140017790 | CONTROL AGENT FOR CONTROLLING UNDIFFERENTIATED STATE AND USE THEREOF - The present invention aims to provide a novel undifferentiated state-control agent that maintains and/or improves an undifferentiated state of undifferentiated cells. CCL2 or a protein containing a functional domain thereof is used as the undifferentiated state-control agent. By culturing undifferentiated cells in the presence of the control agent, it is possible to maintain and/or improve an undifferentiated state of the undifferentiated cells. Examples of the undifferentiated cells include embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells). The origin of the cells is not particularly limited, and may be a human, mouse, or the like, for example. | 01-16-2014 |
20140024114 | COMPOSITIONS AND METHODS FOR PROMOTING THE GENERATION OF DEFINITIVE ENDODERM - Certain embodiments disclosed herein are directed to a method of producing endoderm cells, such as definitive endoderm cells by exposing stem cells such as embryonic stem cells or induced pluripotent stem (iPS) cells to an effective amount of at least one compound described herein to differentiate the stem cells into the endoderm cells such as definitive endoderm cells. Differentiated endoderm cells produced by the methods disclosed herein can be differentiated into pancreatic epithelium, and other endoderm derivatives such as thymus, liver, stomach, intestine and lung. Another aspect of the present invention relates to a method of producing pancreatic progenitor cells, such as Pdx1-positive pancreatic progenitor cells by exposing endoderm cells, such as definitive endoderm cells to an effective amount of at least one compound described herein to differentiate the definitive endoderm cells into Pdx1-positive pancreatic progenitor cells. Kits and compositions comprising Pdx1-positive pancreatic progenitor produced using the methods are also described. | 01-23-2014 |
20140045265 | ROBUST AND EFFICIENT DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MULTIPOTENT VASCULAR PROGENITORS - Provided herein are methods and compositions for generating vascular progenitor cells that can in turn be differentiated into endothelial, smooth muscle, and hematopoietic cells. The present methods provide a faster, safer, and more efficient in vitro differentiation program than existing methodologies. The differentiated cells can be used for research and therapeutic applications, such as cell transplantation. | 02-13-2014 |
20140045266 | METHODS OF CULTURING CELLS IN A MEDIUM COMPRISING TRANSFORMING GROWTH FACTOR BETA 1 AND BASIC FIBROBLAST GROWTH FACTOR - The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells. | 02-13-2014 |
20140057352 | METHODS FOR THE IDENTIFICATION OF AGENTS THAT INHIBIT MESENCHYMAL-LIKE TUMOR CELLS OR THEIR FORMATION - The present invention provides tumor cell preparations for use as models of the EMT process for use in the identification of anti-cancer agents, wherein said tumor cell preparations comprise cells of the epithelial tumor cell line H1650, which are stimulated by receptor ligands to induce EMT, or which have been engineered to inducibly express a protein that stimulates EMT. The present invention also provides methods of identifying potential anti-cancer agents by using such tumor cell preparations to identify agents that inhibit EMT, stimulate MET, or inhibit the growth of mesenchymal-like cells. Such agents should be particularly useful when used in conjunction with other anti-cancer drugs such as EGFR and IGF-1R kinase inhibitors, which appear to be less effective at inhibiting tumor cells that have undergone an EMT. | 02-27-2014 |
20140080210 | Treatment of Pluripotent Cells - The present invention is directed to methods to treat pluripotent cells, whereby the pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of GSK-3B enzyme activity. | 03-20-2014 |
20140087464 | MULTIPOTENT NEURAL CELLS - The inventions disclosed herein are based on the identification of novel cell populations derived from human embryonic stem cells and other pluripotent cells. The inventive cell populations may be used for cell therapies for the treatment of various neurological diseases and as substrates in pharmacological assays. | 03-27-2014 |
20140093960 | CELL DIFFERENTIATION INDUCER AND DIFFERENTIATION INDUCING METHOD - The objective of the present invention is to provide a cell differentiation inducer which is a low-molecular compound, which can be chemically synthesized easily and which can efficiently induce a differentiation of an undifferentiated cell into a nervous system cell with high selectivity. In addition, the objective of the present invention is to provide use of a specific catechol derivative for efficiently inducing a differentiation of an undifferentiated cell into a nervous system cell with high selectivity, and a method for efficiently inducing a differentiation of an undifferentiated cell into a nervous system cell with high selectivity using a specific catechol derivative. The cell differentiation inducer of the present invention is characterized in comprising the catechol derivative having the specific chemical structure. | 04-03-2014 |
20140113372 | CELL CULTURE SUBSTRATE, AND CELL CULTURING METHOD USING THE SUBSTRATE AND METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS USING THE SUBSTRATE - [Problem] To provide a cell culture substrate, and a cell culturing method using the substrate and a method for inducing differentiation of pluripotent stem cells using the substrate, which allow culturing of pluripotent stem cells and allow differentiation of pluripotent stem cells into a specified cell species, particularly neural and neural progenitor cells, at a high purity. | 04-24-2014 |
20140127806 | MODIFIED LAMININ AND USE THEREOF - Provided are a modified laminin having a cell-growth regulatory molecule bound to at least one site selected from the α chain N-terminus, the α chain C-terminus, the β chain N-terminus and the δ chain N-terminus of laminin or a heterotrimeric laminin fragment, a method for culturing cells in the presence of the modified laminin, a method for establishing iPS cells in the presence of the modified laminin, and a culture substrate coated with the modified laminin. Human stem cells cultured in a xeno-free environment with the use of the modified laminin of the present invention can be provided as highly safe human stem cells applicable to regenerative medicine. | 05-08-2014 |
20140127807 | Method for Promoting Differentiation of Pluripotent Stem Cells into Cardiac Muscle Cells - The present invention relates to a composition for promoting differentiation of pluripotent stem cells into cardiac muscle cells, and a method for inducing differentiation of pluripotent stem cells into cardiac muscle cells and a method for preparing cardiac muscle cells | 05-08-2014 |
20140134732 | Simplified Compositions and Methods for Generating Neural Stem Cells From Human Pluripotent Stem Cells - Simplified methods and compositions for directed differentiation of human pluripotent stem cells into neural stem cells are described. Methods and compositions for deriving neural stem cells from human pluripotent stem cells under defined, xeno-free conditions are also described. | 05-15-2014 |
20140134733 | CHEMICALLY DEFINED PRODUCTION OF CARDIOMYOCYTES FROM PLURIPOTENT STEM CELLS - Methods are provided for producing a cardiomyocyte population from a mammalian pluripotent stem cell population. Aspects of the methods include using a Wnt signaling agonist and antagonist, each in minimal media, to modulate Wnt signaling. Also provided are kits for practicing the methods described herein. | 05-15-2014 |
20140154805 | ENHANCERS OF INDUCED PLURIPOTENT STEM CELL REPROGRAMMING - Described herein, inter alia, are methods and compositions useful for induced pluripotent stem cell reprogramming. | 06-05-2014 |
20140170751 | METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL - The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved. | 06-19-2014 |
20140178994 | Methods and Compositions for In Vitro and In Vivo Chondrogenesis - Aspects of the present invention include methods and compositions related to the production, identification and use of embryonic progenitor cell lines that are capable of undergoing chondrogenesis. A number of exemplary chondrogenic cell lines derived from primordial stem cells are disclosed. The chondrogenic cell lines described herein are robust, can expand for >40 passages, and have site-specific purity, thus providing for compositions and methods of producing diverse cartilage types with unique molecular compositions for use in research and therapy. | 06-26-2014 |
20140186952 | SCAFFOLD-FREE TISSUE CONSTRUCTS - A tissue construct comprising includes a self-assembled, scaffold-free, high-density cell aggregate. The cell aggregate includes a plurality of cells and a plurality of biocompatible and degradable nanoparticles and/or microparticles that are incorporated within the cell aggregate. The nanoparticles and/or microparticles act as a bulking agent and/or provide bioactive agents or signals within the cell aggregate to increase the cell aggregate size and/or thickness and improve the mechanical properties of the cell aggregate and/or regulate cell function within the aggregate allowing the cell aggregate to be readily manipulated and formed into tissue constructs with defined architectures and potential tissue specific functionality. | 07-03-2014 |
20140186953 | Differentiation of Human Embryonic Stem Cells Into Pancreatic Endocrine Cells Using HB9 Regulators - The present invention provides methods to promote differentiation of pluripotent stem cells to pancreatic endoderm cells expressing PDX1, NKX6.1, and HB9. In particular, the methods encompass culturing Stage 4 to Stage 6 cells with a thyroid hormone (e.g. T3), an ALK5 inhibitor, or both. | 07-03-2014 |
20140186954 | Optimized Methods for Differentiation of Cells Into Cells with Hepatocyte and Hepatocyte Progenitor Phenotypes, Cells Produced by the Methods, and Methods for Using the Cells - The invention is directed to methods for culturing cells so that the cells are induced to differentiate into cells that express hepatocyte phenotypes and hepatocyte progenitor phenotypes. More particularly, the invention relates to methods for culturing cells so that the cells are induced to differentiate into cells that express a definitive endodermal phenotype, a liver-committed endodermal phenotype, a hepatoblast phenotype, and hepatocyte phenotype. The invention is also directed to cells produced by the methods of the invention. The cells are useful, among other things, for treatment of liver deficiency, liver metabolism studies, and liver toxicity studies. | 07-03-2014 |
20140186955 | GENERATION OF NEURONAL CELLS FROM PLURIPOTENT STEM CELLS - The invention relates to in vitro methods for differentiating mammalian pluripotent stem cells into cells displaying a neuronal phenotype, more particularly into cortical-type neurons including inter alia pyramidal neurons and cortical inhibitory interneurons. The invention further encompasses so-obtained neuronal cells and cell population, compositions comprising such, and further uses of said neuronal cells and cell population. | 07-03-2014 |
20140193909 | Direct Differentiation of Cardiomyocytes From Embryonic Stem Cells - The present invention relates to the induction of differentiation in human embryonic stem (hES) cells to cardiomyocytes and factors such as prostaglandin I2 (PGI2), Fgf 9, Bmp6, Bmp4, Scf, Igf2, and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from hES cells is also provided wherein the media contains these factors. Genes that are upregulated in the process of cardiomyocyte differentiation are also provided. | 07-10-2014 |
20140193910 | METHODS FOR PRODUCING PANCREATIC PRECURSOR CELLS AND USES THEREOF - The present invention is directed to methods for readily propagating somatic pancreatic precursor cells. The methods comprise isolating cells from intact pancreatic samples and enhancing guanine nucleotide (GNP) biosynthesis in cultures comprising these cells, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the cells, thereby generating pancreatic precursor cells. | 07-10-2014 |
20140212970 | METHOD AND KIT FOR CULTURING STEM CELLS - A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost. | 07-31-2014 |
20140212971 | Mast Cell Culture and Methods to Produce the Culture - The present invention relates to mast cell cultures that are derived from hematopoietic progenitors and the use thereof. The invention describes a method for generating in-vitro cultures of human mast cells with functional phenotype of connective tissue-type mast cells. By monitoring the levels of chemokines released into the medium, such mast cell cultures can be used as a cell-based assay to assess regulation of mast cell functions and pharmacological activities of tryptase inhibitors. | 07-31-2014 |
20140212972 | PROCESS FOR PRODUCING CYTOTOXIC LYMPHOCYTE - The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof. | 07-31-2014 |
20140220686 | METHOD FOR UNDIFFERENTIATED GROWTH OF MESENCHYMAL STEM CELL AND METHOD FOR CONCENTRATION OF MESENCHYMAL STEM CELL - A method in accordance with the present invention includes the steps of: concentrating cord blood-derived mesenchymal stem cells; and causing the mesenchymal stem cells thus concentrated to grow with use of a particular factor while maintaining undifferentiated state of the mesenchymal stem cells. | 08-07-2014 |
20140234963 | EFFICIENT INDUCTION OF DEFINITIVE ENDODERM FROM PLURIPOTENT STEM CELLS - The present invention relates to a method to differentiate pluripotent stem cells to a primitive streak cell population, in a stepwise manner for further maturation to definitive endoderm. | 08-21-2014 |
20140234964 | Novel Methods and Formulations for Orthopedic Cell Therapy - Aspects of the present invention include methods and compositions related to the production and use of clonal lineages of embryonic progenitor cell lines derived from differentiating cultures of primordial stem cells, in particular, said methods and compositions relate to methods of differentiating cells in the presence of BMP family mambeis of growth factors and the applications of said cell lines in the treatment of degenerative orthopedic diseases such as osteoarthritis, | 08-21-2014 |
20140234965 | TRIAZINE DERIVATIVES AS DIFFERENTIATION CATALYSTS - A method of inducing differentiation of mammalian cells into cardiomyocyte-like cells by contacting the mammalian cell with a triazine compound of formula (I), wherein X is independently —NR | 08-21-2014 |
20140242695 | PROTEIN-INDUCED PLURIPOTENT CELL TECHNOLOGY AND USES THEREOF - A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof. | 08-28-2014 |
20140256042 | METHOD OF PRODUCING ERYTHROCYTES WITHOUT FEEDER CELLS - Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place. | 09-11-2014 |
20140256043 | SMALL CHEMICAL COMPOUND WHICH PROMOTES INDUCTION OF DIFFERENTIATION OF STEM CELLS INTO INSULIN-PRODUCING CELLS AND METHOD FOR INDUCING DIFFERENTIATION OF STEM CELLS INTO INSULIN-PRODUCING CELLS USING SAID SMALL CHEMICAL COMPOUND - A purpose of the present invention is to provide a small chemical compound which especially promotes induction of the differentiation of ES cells into insulin-producing cells. Another purpose of the present invention is to provide: a method for inducing the differentiation of ES cells into insulin-producing cells using the compound; and an agent for promoting induction of the differentiation into insulin-producing cells. Another purpose of the present invention is to provide the thus-induced insulin-producing cells. Provided are: an agent for promoting induction of the differentiation of stem cells derived from a mammal into insulin-producing cells, which contains a compound that is selected from the group consisting of dopamine metabolism inhibitors, serotonin metabolism inhibitors, acetylchotine, acetylcholine degrading enzyme inhibitors and acetylcholine receptor activators; and a method for inducing differentiation using the agent for promoting induction of differentiation. | 09-11-2014 |
20140273219 | DIFFERENTIATED CELLULAR COMPOSITIONS AND METHODS FOR THEIR PREPARATION AND USE - The present invention relates to methods for culturing progenitor cells to differentiate primarily into a single population of cells with the same phenotype, and to compositions thereof. In particular, it relates to methods for culturing liver progenitor cells to differentiate primarily into a single population of hepatocyte-like cells, and to compositions thereof. | 09-18-2014 |
20140273220 | Self-Organized Vascular Networks from Human Pluripotent Stem Cells in a Synthetic Matrix - A bicellular vascular population derived from human pluripotent stem cells (hPSCs) undergoes morphogenesis and assembly in a synthetic hydrogel. It is shown that hPSCs can be induced to co-differentiate into early vascular cells (EVCs) in a clinically-relevant strategy dependent upon Notch activation. These EVCs mature into ECs and pericytes, and self-organize to form vascular networks in an engineered matrix. Upon in vivo implantation, multicellular human vascular networks are functionally perfused. Thus, a derived bicellular population is exploited for its intrinsic self-assembly capability to create functional microvasculature in a deliverable matrix. | 09-18-2014 |
20140287504 | ENDODERMAL CELLS FROM PLURIPOTENT CELLS - The present inventions describes a method that, starting from pluripotent cells, leads to the obtainment, in a reproducible and efficient manner, of endodermal cells precursor. These cells reveal useful also for application in the regenerative therapy. | 09-25-2014 |
20140295551 | Protein markers associated bone marrow stem cell differentiation into early progentior cells - A novel cytosolic 58 kd phosphoprotein induced during bone marrow stem cell (BM) differentiation into dendritic cells (DC) during in vitro cultivation with the cytokine GM-CSF by addition of antisera to an 82 kd BM cell surface protein generating cultivatable dendritic progenitor cells (DP). Genes, methods for preparing them as well as early DP have been provided. Potential uses/advantages lie in the study of BM differentiation and innate immunity due to stimulatory/inhibitory DC, contribution of BM and DP to inflammation during infection and carcinogenesis, tumor promotion/regression, identification of BM-derived blood cells, T-cell activation/regulation/tolerance and inflammation. | 10-02-2014 |
20140295552 | SUSPENSION AND CLUSTERING OF HUMAN PLURIPOTENT STEM CELLS FOR DIFFERENTIATION INTO PANCREATIC ENDOCRINE CELLS - The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters. | 10-02-2014 |
20140295553 | IN-VITRO CELL COLONY CULTURE DEVICE AND USE THEREOF - Provided are an in-vitro cell colony culture device and use thereof, the in-vitro cell colony culture device comprising: a micro-patterned template having a micro-patterned cavity, and adhesive hydrogel formed on the lower surface of the micro-patterned template, and the micro-patterned cavity defining the growth space of the cell colony. An upper template can be retained or removed as required to from a monolayer cell micro-pattern or a multiplayer cell micro-cluster. | 10-02-2014 |
20140308745 | BONE MARROW-DERIVED MESENCHYMAL STEM CELLS AS A SOURCE OF NEURAL PROGENITORS - Methods are provided for treating and/or reducing the severity of multiple sclerosis in a human, by administering autologous mesenchymal stem cell-derived neural precursors. Also described is an in vitro method for differentiating mesenchymal stem-cell derived neural precursor oligodendroglial and neuronal cell types. | 10-16-2014 |
20140308746 | SUSTAINED POLYPEPTIDE EXPRESSION FROM SYNTHETIC, MODIFIED RNAS AND USES THEREOF - Described herein are synthetic, modified RNAs for changing the phenotype of a cell, such as expressing a polypeptide or altering the developmental potential. Accordingly, provided herein are compositions, methods, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells. These methods, compositions, and kits comprising synthetic, modified RNAs can be used either to express a desired protein in a cell or tissue, or to change the differentiated phenotype of a cell to that of another, desired cell type. | 10-16-2014 |
20140315305 | METHOD FOR PRODUCING CORNEAL ENDOTHELIAL CELL - The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or iPS cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of TGFb2, insulin, a ROCK inhibitor, and the like. | 10-23-2014 |
20140315306 | CELL CULTURE SUBSTRATE COMPRISING A LAMININ AND A CADHERIN - The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein. | 10-23-2014 |
20140322811 | MEDIUM COMPOSITION FOR REJUVENATING STEM CELLS - The present invention relates to a medium composition for transforming stem cells from an aged person into young stem cells, and more particularly to a medium composition for culturing stem cells, which is used to rejuvenate stem cells from an aged person so as to have characteristics similar to those of the stem cells of young people, and to a method for rejuvenating stem cells, which comprises culturing stem cells from an aged person in the medium composition. According to the present invention, even mesenchymal stem cells collected from over 60 years old patients can be transformed into young mesenchymal stem cells having high differentiation ability, high telomerase activity, and high ability to express stem cell markers. Thus, the present invention can significantly increase the efficacy of cell therapy employing mesenchymal stem cells. | 10-30-2014 |
20140329321 | METHODS AND COMPOSITIONS FOR CULTURING ENDODERM PROGENITOR CELLS IN SUSPENSION - Provided herein are methods for the in vitro differentiation of induced pluripotent stem cells, which have been expanded and/or maintained under defined conditions, into endodermal precursor cells (EPCs) that are capable of producing mono-hormonal beta cells. | 11-06-2014 |
20140329322 | METHOD OF DIFFERENTIATING GLIOBLASTOMA CELLS - A method of increasing differentiation of a cell line of glioblastoma cells is disclosed. A platelet-rich plasma (PRP) composition is prepared. The glioblastoma cells are cultured in the PRP composition for a time period sufficient for differentiation of the glioblastoma cells. | 11-06-2014 |
20140335615 | METHODS OF USING MECHANICAL FORCE WITH SOMATIC AND PLURIPOTENT CELLS - Provided are methods useful in increasing efficiency of biotransport, reprogramming or altering a cell's state of differentiation, and maintenance of cells in an undifferentiated state. | 11-13-2014 |
20140363890 | THREE-DIMENSIONAL STRUCTURES FOR CELL OR TISSUE CULTURE - Among others, the present invention provides devices for cell or tissue culture, comprising a three-dimensional structure, which further includes fibrils with beads and/or particles. The present invention also relates to novel methods for manufacturing devices for cell or tissue culture. | 12-11-2014 |
20140377865 | DERIVATION OF EMBRYONIC STEM CELLS - This present invention provides novel methods for deriving embryonic stem cells, those cells and cell lines, and the use of the cells for therapeutic and research purposes without the destruction of the embryo. It also relates to novel methods of establishing and storing an autologous stem cell line prior to implantation of an embryo, e.g., in conjunction with reproductive therapies such as IVF. | 12-25-2014 |
20150011003 | CULTURE MEDIUM FOR PROLIFERATING STEM CELL, WHICH CONTAINS SULFATED COMPOUND - A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells. | 01-08-2015 |
20150017723 | PROCESS OF EXPANDING T CELLS - The present disclosure relates to a novel process for expanding T cells, such as autologous T cells, cell populations therefrom, pharmaceutical compositions comprising the said cell populations and use of the cells and compositions for treatment, particular the treatment or prophylaxis of virus infection and/or cancer, for example in immune compromised or immune competent human patients. | 01-15-2015 |
20150017724 | METHOD FOR GUIDING THE DERIVATION OF ENDOTHELIAL CELLS FROM HUMAN PLURIPOTENT STEM CELLS EMPLOYING TWO-DIMENSIONAL, FEEDER-FREE DIFFERENTIATION - This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, by plating a single-cell suspension of PSCs onto a suitable surface such as type IV collagen and culturing the cells with VEGF after which ECs can be harvested. A preferred embodiment of the method first cultures the cells without VEGF and then sequentially cultures the cells with VEGF. Differentiation can be enhanced by adding an inhibitor of transforming growth factor β to the culturing with VEGF. | 01-15-2015 |
20150044770 | METHOD FOR SELECTIVE CELL ATTACHMENT/DETACHMENT, CELL PATTERNIZATION AND CELL HARVESTING BY MEANS OF NEAR INFRARED RAYS - The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization. | 02-12-2015 |
20150072425 | METHODS OF GENERATING NATURAL KILLER CELLS - Provided herein are methods of producing natural killer cells using a two-step expansion and differentiation method. Also provided herein are methods of suppressing tumor cell proliferation, of treating individuals having cancer or a viral infection, comprising administering the NK cells produced by the method to an individual having the cancer or viral infection. | 03-12-2015 |
20150072426 | FGF-2 Variants and Methods of Use Thereof - A method of treating an individual (i) having abnormal bone; or (ii) afflicted with a disease or disorder related to normal or abnormal FGF receptors or a skeletal disorder; or (iii) having dysplasic bone. The method includes administering to the individual a pharmaceutical composition comprising a therapeutically effective amount of a fibroblast growth factor 9 (FGF-9) variant comprising at least one amino acid substitution in the beta 8-beta 9 loop, wherein said FGF-9 variant incorporates one of the amino acid sequences set forth in SEQ ID NO: 11, 13, 14, 15, 16 or 17. | 03-12-2015 |
20150072427 | Dedifferentiation and Reprogramming of Cells - The invention is directed to methods for reprogramming somatic cells to a less differentiated state. In particular, the invention is directed to methods for reprogramming amnion epithelial cells (AEC) including amnion-derived cells (ADC) and Amnion-derived Multipotent Progenitor cells (AMP cells) to a less differentiated state. The invention is further directed to compositions comprising reprogrammed AEC, ADC and AMP cells, and uses thereof. | 03-12-2015 |
20150087060 | METHOD FOR INDUCING ERYTHROPOIETIN-PRODUCING CELL - An object of the present invention is to provide a method for inducing the differentiation of erythropoietin-producing cells from human pluripotent stem cells. The above object is achieved by providing a method for inducing the differentiation of erythropoietin-producing cells from human pluripotent stem cells using a medium containing a specific growth factor and a specific compound. | 03-26-2015 |
20150087061 | Methods of Preparing Tissues for Xenotransplantation Using Alpha-galactosidases - This invention relates to novel α-galactosidases for the enzymatic removal of the immunodominant monosaccharides on blood products and tissues. Specifically this invention provides a novel family of α3 glycosidases, used for the enzymatic removal of type B antigens from blood group B and AB reactive blood products, and the Galili antigen from non-human animal tissues, thereby converting these to non-immunogenic cells and tissues suitable for transplantation. | 03-26-2015 |
20150099300 | USE OF ADIPOSE TISSUE-DERIVED STROMAL CELLS FOR CHONDROCYTE DIFFERENTIATION AND CARTILAGE REPAIR - Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed. | 04-09-2015 |
20150104870 | NEURONAL DIFFERENTIATION PROMOTER - The present invention provides an agent for promoting neuronal differentiation of neural stem cells, which contains a p38 inhibitor. In addition, the present invention provides a method of promoting neuronal differentiation of neural stem cells, which includes cultivating neural stem cells in the presence of a p38 inhibitor. Neural stem cells are gliogenic or neurogenic. The p38 inhibitor is a nucleic acid which hybridizes to DNA or mRNA encoding p38 under physiological conditions, thereby inhibiting the transcription and/or translation thereof, an expression vector of the nucleic acid, a low molecular p38 inhibitor and the like. | 04-16-2015 |
20150104871 | MEDIUM COMPRISING TRANSFORMING GROWTH FACTOR BETA 1 AND BASIC FIBROBLAST GROWTH FACTOR - The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells. | 04-16-2015 |
20150125956 | COMPOSITION FOR MATURING DENDRITIC CELLS, AND METHOD FOR PREPARING ANTIGEN-SPECIFIC DENDRITIC CELLS USING SAME - The present invention relates to a composition for maturing dendritic cells, comprising, as a maturation-promoting factor, Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ), Prostaglandin E2 (PGE2), Picibanil (OK432) and/or Poly IC. The composition for maturing dendritic cells of the present invention may have the effects of not only improving the ability of dendritic cells to induce an immune response, but also of decreasing the antigen non-specific immune response of dendritic cells and increasing antigen-specific immune response of dendritic cells, thus maximizing the effects of immunotherapy. | 05-07-2015 |
20150125957 | CELLULAR RESPONSE TO SURFACE WITH NANOSCALE HETEROGENEOUS RIGIDITY - An elastomeric substrate comprises a surface with regions of heterogeneous rigidity, wherein the regions are formed by exposing the elastomeric substrate to an energy source to form the regions such that the regions include a rigidity pattern comprising spots. | 05-07-2015 |
20150132849 | EX VIVO MATURATION OF ISLET CELLS - The invention relates to methods for promoting maturation of islet cells from pre-weaned mammals for the purpose of optimizing the islets for their use as donor tissue for xenotransplantation. The method of the invention removes the pancreas from donor animals and reduces the pancreas tissue to fragments that are greater than the size of an intact islet while retaining islets in their whole, insulin-producing condition. The method of the invention also serially cultures the digested tissue in novel maturation media that enhance the glucose responsiveness of the cultured islets, and selects islets that are sufficiently glucose-responsive for use in transplantation procedures. | 05-14-2015 |
20150132850 | STEM CELLS FROM THE MAMMALIAN NEURAL PLATE - The present invention relates to methods for deriving novel stem cells from the mammalian early neural plate. | 05-14-2015 |
20150140657 | METHOD OF GENERATING NATURAL KILLER CELLS AND DENDRITIC CELLS FROM HUMAN EMBRYONIC STEM CELL-DERIVED HEMANGIOBLASTS - This invention provides methods of generating natural killer (NK) cells and dendritic cells (DCs). The methods utilize human hemangioblasts as intermediate cells to generate the NK cells and DCs. In various embodiments, the methods do not require the use of stromal feeder layers. | 05-21-2015 |
20150140658 | LINEAGE REPROGRAMMING TO INDUCED CARDIAC PROGENITOR CELLS (iCPC) BY DEFINED FACTORS - Animal cells, notably adult fibroblasts, are advantageously reprogrammed in direct lineage reprogramming methods using defined factors to produce proliferative and multipotent induced cardiac progenitor cells (iCPC). The iCPC thus produced can be differentiated under suitable differentiation conditions to cardiac lineage cells including cardiomyocytes, smooth muscle cells, and endothelial cells, as evidenced by expression of lineage specific markers. Sets of factors effective in combination to reprogram the fibroblasts can include a set that includes some or all of 5 factors (Mesp1, Baf60c, Nkx2.5, Gata4, Tbx5), a set that includes some or all of 11 factors (Mesp1, Mesp2, Gata4, Gata6, Baf60c, SRF, Isl1, Nkx2.5, Irx4, Tbx5, Tbx20), a set that includes some or all of 18 factors (T, Mesp1, Mesp2, Tbx5, Tbx20, Isl1, Gata4, Gata6, Irx4, Nkx2.5, Hand1, Hand2, Tbx20, Tbx18, Tip60, Baf60c, SRF, Hey2), and a set that includes some or all of 22 factors (T, Mesp1, Mesp2, Tbx5, Tbx20, Isl1, Gata4, Gata6, Irx4, Nkx2.5, Hand1, Hand2, Tbx20, Tbx18, Tip60, Baf60c, SRF, Hey2, Oct4, Klf4, Sox2, L-myc). | 05-21-2015 |
20150147807 | HIGH-THROUGHPUT IMAGE-BASED CHEMICAL SCREENING IN ZEBRAFISH BLASTOMERE CELL CULTURE - Disclosed are methods of inducing differentiation of stem into myogenic cells without gene manipulation and for inducing proliferation of satellite cells. The cells can be used as a source of cells for transplantation in a subject in need thereof. Also disclosed is a screening assay for screening test compounds using blastomere cultures. | 05-28-2015 |
20150291931 | MESENDODERM AND MESODERM CELL POPULATIONS - The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy. | 10-15-2015 |
20150291933 | CELL DIFFERENTIATION - Provided is a method of producing neural precursor cells, in which an inhibitor of E-cadherin activity is provided to a population of the cells having neural potential, cell stress is induced among the population of cells; and the surviving cells are cultured until neural precursor cells are produced. Also provided is a method of adapting a cell in vitro for therapeutic use, in which an inhibitor of E-cadherin activity is provided to a population of cells having neural potential, cell stress is induced among the population of cells, and the surviving cells are cultured until neural precursor cells are produced. This method may optionally additionally involve culturing the neural precursor cells until neural cells are produced and formulating the neural precursor cells or neural cells in a composition suitable for administration to a patient. The invention also provides cells produced by these methods. The methods may be practiced on stem cells, particularly iPSCs. The cells and methods have utility in applications including stratified medicine. | 10-15-2015 |
20150299651 | CELL CULTURE SUPPLEMENTS - The invention relates to cell culture supplements based on a platelet rich plasma fraction and a platelet poor plasma fraction. The supplements increase cell proliferation rate, improve selection of clonogenic cells, proliferation of cells from biopsies from elderly patients, maintenance of cell differentiation potential, and in vitro expansion of cell cultures also starting from an extremely low number of initially plated cells. The supplements may be freeze-dried and kept for long period of time as a quality controlled “off the shelf” product. | 10-22-2015 |
20150299660 | DIFFERENTIATION OF HUMAN FIBROBLAST CELLS - The present invention is based in part on methods of differentiating fibroblast cells into adipocytes, osteocytes and chondrocytes. Additionally, the present invention provides agents and kits useful for differentiating fibroblast cells in adipocytes, osteocytes and chondrocytes. Further, the present invention provides for enhanced extracellular matrix deposition using complex sugars. | 10-22-2015 |
20150315545 | Method of rapidly inducing large-scale and high-purity mesenchymal stem cells to transdetermine into hematopoietic stem cells - A method of rapidly inducing large-scale and high-purity mesenchymal stem cells to transdetermine into hematopoietic stem cells is provided with the steps of preparing homogeneous medium of mesenchymal stem cells; combining a plurality of small RNA molecules; assembling and transfecting nanoparticles of nucleic acids and polypeptides; inducing and amplifying medium of post-transdetermined hematopoietic stem cells; and activating a plurality of hemopoiesis-related genes. | 11-05-2015 |
20150329828 | Use of Small Molecules to Enhance Mafa Expression in Pancreatic Endocrine Cells - The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface. | 11-19-2015 |
20150329831 | Method for Culturing Pluripotent Stem Cells - Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. | 11-19-2015 |
20150329832 | CULTURE METHOD FOR STABLE PROLIFERATION OF PLURIPOTENT STEM CELL WHILE MAINTAINING UNDIFFERENTIATED STATE - Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state. | 11-19-2015 |
20150344842 | METHOD FOR PRODUCTION OF DECELLULARIZED BIOLOGICAL MATERIAL AND THE DECELLULARIZED BIOLOGICAL MATERIAL PREPARED THEREFROM - The invention provides a method for decellularization of a biological material to obtain a decellularized biological material. Compared to untreated biological material, the content of DNA of the decellularized biological material of the invention decreases to a low level and there is no significant reduction of glycosaminoglycan and collagen. The invention also provides a decellularized biological material prepared from the method of the invention and a support comprising the decellularized biological material. | 12-03-2015 |
20150344845 | METHODS, KITS, AND COMPOSITIONS FOR STEM CELL SELF-RENEWAL - The present invention relates to methods and kits for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods, kits, and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population. | 12-03-2015 |
20150344848 | SULFATED DEPOLYMERIZED DERIVATIVES OF EXOPOLYSACCHARIDES (EPS) FROM MESOPHILIC MARINE BACTERIA, METHOD FOR PREPARING SAME, AND USES THEROF IN TISSUE REGENERATION - The invention relates to certain low-molecular weight sulphated polysaccharide derivatives of marine native exopolysaccharides (EPSs) excreted by mesophilic marine bacteria from a deep hydrothermal environment, wherein said derivatives can be obtained by means of a method which comprises a step of free radical depolymerisation of said native EPSs followed by a step of sulphating the resulting depolymerised derivatives. The present invention further relates to the use of said low-molecular weight sulphated polysaccharide derivatives as a wound-healing agent, particularly for preparing pharmaceutical compositions suitable for treating or preventing diseases of the connective tissues and particularly skin and gum tissues. The figure demonstrates how polysaccharide derivative GY 785 DRS according to the invention can stimulate fibroblast proliferation in latticed or reconstructed connective tissues at a concentration of 10 μg (m) g/ml. | 12-03-2015 |
20150344849 | EX VIVO CULTURE, PROLIFERATION AND EXPANSION OF INTESTINAL EPITHELIUM - Methods are provided for long term culture of mammalian intestinal cells. Cultures are initiated with fragments of mammalian intestinal tissue, which are then maintained embedded in a gel substrate that provides an air-liquid interface. Intestinal epithelium in cultures of the invention can be continuously grown for extended periods of time. Mammalian intestinal cells cultured by the methods of the invention recapitulate features of intestinal growth in vivo. | 12-03-2015 |
20150353895 | Differentiation of Human Embryonic Stem Cells into Single Hormonal Insulin Positive Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells. | 12-10-2015 |
20150361391 | METHOD FOR INDUCING TAILORED PLURIPOTENT STEM CELLS USING EXTRACT OF PLANT STEM CELLS OR PLANT DEDIFFERENTIATED STEM CELLS, AND PLURIPOTENT STEM CELLS PRODUCED BY MEANS OF THE METHOD - A method for inducing tailored pluripotent stem cells by reprogramming differentiated cells of an adult by using an extract of plant stem cells and plant dedifferentiated stem cells (callus); pluripotent stem cells produced by means of the method; and a cell therapy agent comprising the pluripotent stem cells are disclosed. It is possible to overcome ethical problems since eggs are not used in the making of pluripotent stem cells having abilities like embryonic stem cells. It is possible to produce pluripotent stem cells of ensured safety because a plant stem cell extract which above all has been verified to be harmless to the body is used. It is possible to develop an immunocompatible cell therapy agent tailored to different individuals. | 12-17-2015 |
20160002586 | Megakaryocyte and Platelet Production from Stem Cells - Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein. | 01-07-2016 |
20160002596 | COMPOSITION FOR EX VIVO CULTURING OF A CORNEAL ENDOTHELIAL CELL AND A METHOD FOR USING THE COMPOSITION - A composition for ex vivo culture of corneal endothelial cell and a method for used the composition are disclosed. The composition or the method is applied to ex vitro culture of the corneal endothelial cell to maintain the corneal endothelial cell in a hexagonal phenotype, and to prevent the corneal endothelial cell from endothelial-mesenchymal transformation (EnMT). Therefore, the corneal endothelial cell can maintain normal phenotype and function and can be used in the further application. The composition at least comprises an effective concentrated matrix metalloproteinase inhibitor (MMPI). | 01-07-2016 |
20160010056 | METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS | 01-14-2016 |
20160010060 | METHODS AND PRODUCTS FOR TRANSFECTION | 01-14-2016 |
20160024471 | METHOD FOR MANUFACTURING AN IMMUNE CELL-CONTAINING COMPOSITION, AND A CANCER-TREATING COMPOSITION - To provide a method in which mononuclear cells are differentiated into a good balance of NK cells, NKT cells, and T cells, and said cells are made to proliferate. The present invention provides a method for manufacturing an immune cell-containing composition, said method including the following steps: a first step in which mononuclear cells are cultured in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15; and a second step, after the first step, in which culturing is performed under conditions that make the mononuclear cells preferentially differentiate into cytotoxic T cells. This method may also include a third step, after the second step, in which culturing is performed in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15. | 01-28-2016 |
20160032242 | A ROLE FOR THE PERLMAN SYNDROME EXONUCLEASE DIS3L2 IN THE LIN28-LET-7 PATHWAY - The 3′-5′ exonuclease, Dis3l2, is responsible for the decay of uridylated pre-let-7 miRNA. Biochemical reconstitution assays revealed that 3′ oligouridylation stimulates Dis3l2 activity in vitro, and knockdown of Dis3l2 in mouse embryonic stem cells leads to the stabilization of pre-let-7 miRNA. These Dis3l2-depleted stem cells displayed elevated expression of pluripotency genes and delayed differentiation. The present disclosure establishes 3′ oligouridylation as an RNA decay signal for Dis3l2 and identifies the first physiological RNA substrate of this exonuclease. | 02-04-2016 |
20160032244 | Method of inducing cellular differentiation using the Notch3 receptor intracellular domain - The present invention relates to the intracellular domain of Notch3 that can activate signaling and initiate transcription, thereby initiating cellular differentiation in general, and neuronal differentiation in particular. The present invention includes the use of polynucleotide sequences that code, entirely or partially, for the intracellular domain of Notch3, for the purpose of inducing cellular differentiation. The present invention includes the use of Notch3 intracellular domain polynucleotide or polypeptide sequences for the purpose of treating diseases or disorders, by inducing cellular differentiation. | 02-04-2016 |
20160032247 | DIFFERENTIATION-INDUCING CULTURE MEDIUM ADDITIVE AND USE THEREOF - Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid. | 02-04-2016 |
20160032249 | COMPOSITIONS AND METHODS FOR PROMOTING THE GENERATION OF ENDOCRINE CELLS - Disclosed herein are compositions and methods for the generation of insulin positive β cells, for example by exposing Pdx+ pancreatic progenitor cells to one or more compounds, and compositions and kits comprising isolated populations of insulin positive cells. | 02-04-2016 |
20160032250 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon. | 02-04-2016 |
20160040130 | DIFFERENTIATION OF PLURIPOTENT STEM CELLS - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method utilizing a CYP26A inhibitor to produce a population of pancreatic endocrine precursor cells. | 02-11-2016 |
20160046904 | Method For Culturing Hepatoblast-Like Cells And Culture Product Thereof - Provided is a method of stably maintaining and culturing “hepatoblast-like cells” generated during a differentiation-inducing process from pluripotent stem cells to hepatocytes. The present invention also provides a culture product obtained by the culture method. The hepatoblast-like cells can be maintained and cultured stably by bringing a laminin into contact with the hepatoblast-like cells. The method of the present invention which uses a laminin makes it possible for the first time to culture, maintain, and proliferate the hepatoblast-like cells. Desired mature cells such as mature hepatocytes and bile duct epithelial cells can be generated in a short time period and can be acquired at a desired timing by maintaining the hepatoblast-like cells. Further, the resulting cultured hepatoblast-like cells were demonstrated to be capable of being induced to differentiate into mature hepatocytes, mature cholangiocytes, bile duct epithelial cells, and the like. Thus, a substantially pure culture product of the present invention can be utilized as a composition for transplantation for use in regeneration of hepatocytes and/or bile duct epithelial cells. | 02-18-2016 |
20160046906 | Generation Of Inner Ear Cells - Methods for generating cells of the inner ear, e.g., hair cells and supporting cells, from stem cells, e.g., mesenchymal stem cells, are provided, as well as compositions including the inner ear cells. Methods for the therapeutic use of the inner ear cells for the treatment of hearing loss are also described. | 02-18-2016 |
20160053225 | METHOD FOR CELL CULTURE - A method for stem or progenitor cell culture. More precisely, the invention relates to a method for cell culture using one or more IαI (inter-alpha trypsin inhibitor or Inter-alpha inhibitor) protein(s) or part(s) thereof as a component in a cell culture media or a coating on a cell culture surface material. Furthermore the invention relates to a cell culture media and a cell culture coating/matrix provided with one or more IαI proteins(s) or part(s) thereof. | 02-25-2016 |
20160053230 | Culture Medium And Method For Inducing Differentiation of Pluripotent Stem Cells To Hepatoblasts - Provided are a method of inducing differentiation of pluripotent stem cells, such as induced pluripotent stem cells (hereinafter abbreviated as iPS cells), to hepatocytes in a short period of time, and a substance to be used in the method. Specifically, provided are a method of producing a cell culture formed substantially of hepatoblasts, the method including culturing pluripotent stem cells, such as iPS cells, in a culture medium having a composition shown in Table 1 below, and a culture medium for inducing differentiation of pluripotent stem cells into hepatoblasts, which has a composition shown in Table 1 below. | 02-25-2016 |
20160060595 | METHODS AND MEDIA FOR DIFFERENTIATING EYE CELLS - Here is provided a novel differentiation protocol, which was experimentally shown to give rise to corneal epithelial precursor cells or early pigmented RPE precursor cells in defined and xeno-free conditions. The early precursor cells may be further maturated towards corneal epithelium cells, stratified corneal epithelium or mature RPE cells. Such cells may contribute to treatment and research of corneal and retinal conditions, diseases, pathologies as well as toxicology and drug development. | 03-03-2016 |
20160060601 | MEDIUM COMPOSITION AND METHOD FOR PRODUCING RED BLOOD CELLS USING SAME - The present invention provides a method of producing erythrocytes, including efficiently differentiating hematopoietic stem cells and/or a hematopoietic progenitor cells into erythrocytes by using a medium composition having an effect of homogeneously dispersing the hematopoietic stem cells and/or the hematopoietic progenitor cells and maintaining a floating state. | 03-03-2016 |
20160068812 | METHOD FOR CULTURING SKELETAL MUSCLE FOR TISSUE ENGINEERING - The invention provides a nutrient medium composition and associated methods for lengthening the useful life of a culture of muscle cells. Disclosed is a method of culturing mammalian muscle cells, including preparing one or more carriers coated with a covalently bonded monolayer of trimethoxy-silylpropyl-diethylenetriamine (DETA); verifying DETA monolayer formation by one or more associated optical parameters; suspending isolated fetal rat skeletal muscle cells in serum-free medium according to medium composition 1; plating the suspended cells onto the prepared carriers at a predetermined density; leaving the carriers undisturbed for cells to adhere to the DETA monolayer; covering the carriers with a mixture of medium 1 and medium 2; and incubating. A cell nutrient medium composition includes Neurobasal, an antibiotic-antimycotic composition, cholesterol, human TNF-alpha, PDGF BB, vasoactive intestinal peptides, insulin-like growth factor 1, NAP, r-Apolipoprotein E2, purified mouse Laminin, beta amyloid, human tenascin-C protein, rr-Sonic hedgehog Shh N-terminal, and rr-Agrin C terminal. | 03-10-2016 |
20160068816 | METHOD FOR INDUCING ALVEOLAR EPITHELIAL PROGENITOR CELLS - This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3β inhibitor, (2) a medium containing a BMP inhibitor and a TGFβ inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3β inhibitor. | 03-10-2016 |
20160075997 | KIT OF MEDIUM OF INDUCING AND AMPLIFYING HEMATOPOIETIC STEM CELLS - A kit of medium of inducing and amplifying hematopoietic stem cells, the kit includes medium of inducing and amplifying hematopoietic stem cell, and the medium of inducing and amplifying hematopoietic stem cell includes cell proliferation factors and cell differentiation inhibitors needed in proliferation of hematopoietic stem cells, and the formula of the medium of inducing and amplifying hematopoietic stem cell is: 80-100% (v/v) DMEM/F12 basic medium, 0-1 μg/ml Sonic hedgehog, 0-1 μg/ml Delta1, 0-1 μg/ml Flt3L, 0-1 μg/ml IGFBP, 0-1 μg/ml SCF, 0-1 μg/ml, Angiopoietin, 0-1 μg/ml TPO, 0-1 μg/ml MBP, 0-1 μg/ml LIF, 0-1 μg/ml TGF-β, 0-100 mM MTEPA, and 1-5 mM NAC. | 03-17-2016 |
20160097035 | METHOD FOR PREPARING NK CELLS - The object of the present invention is to develop a technique in which NK cells having a high cytotoxic activity can be prepared with high purity from hematopoietic precursor cells without using a serum or feeder cells of an animal. | 04-07-2016 |
20160108362 | Culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells - The present invention discloses a culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells. The culture medium comprises at least two agents for neural induction, wherein the agents includes a Wnt signal agonist and a Smad2/3 inhibitor and the culture medium is a feeder free culture. The method is to culture pluripotent stem cells in the culture medium to differentiate into neuroepithelial cells. The neuroepithelial cells can further differentiate into mature neurons for practical applications, including regeneration medicine and drug discovery for neural disorders. | 04-21-2016 |
20160115449 | Novel Method for Producing Differentiated Cells - The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed. | 04-28-2016 |
20160115451 | In Vitro Growth of Tissues Suitable to the Formation of Bone and Bone Forming Tissue Formed Thereby - The present invention is directed to a device for the growth of new bone or bone-like tissue under in vitro cell culture conditions. | 04-28-2016 |
20160122716 | METHOD OF EFFICIENTLY INDUCING CARDIOMYOCYTES - The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies. | 05-05-2016 |
20160122718 | CULTURE MEDIUM COMPOSITION FOR MATURATING CARDIOMYOCYTES DERIVED FROM PLURIPOTENT MAMMALIAN STEM CELLS - The current disclosure relates to a culture medium, different methods to generate adult-like cardiomyocytes from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC) using the medium, in particular from stem cells that differentiated into (foetal) cardiomyocytes, and to kits comprising the medium, or the medium together with differentiated (foetal) cardiomyocytes derived from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC). | 05-05-2016 |
20160130552 | METHOD FOR OBTAINING IMMUNO-SUPPRESSIVE DENDRITIC CELLS - The present invention relates to methods for producing immuno-suppressive dendritic cells. The present invention further relates to the use of such cells for treating patients suffering from autoimmune diseases, hypersensitivity diseases, rejection on solid-organ transplantation and/or Graft-versus-Host disease. | 05-12-2016 |
20160137707 | SYNAPTOGENIC PROTEIN TAGGED WITH BIOTIN AND RECONSTITUTION OF ARTIFICIAL SYNAPSE BY USING SAME - Disclosed is a polypeptide containing an extracellular domain of a synaptogenic protein, and a method for manufacturing a nerve cell, a complex containing a biotin tagged at the C-terminus of the polypeptide, an artificial synapse inducer for coupling the composite to a streptavidin (SAV)-coated substrate and a nerve cell. The complex tagged with a biotin at the C-terminus of the polypeptide containing the extracellular domain of the synaptogenic protein, such as neuroligin-1, can display activity by being attached to the SAV-coated substrate to adjust the orientation thereof without help of a supported lipid bilayer. The complex containing an additional RFP between the extracellular domain and the biotin of the synaptogenic protein not only facilitates easier mass-production, quantification, and tracking, but also displays activity of a normal synaptogenic protein, thereby inducing excitatory or inhibitory synaptic differentiation by being fixed to the substrate and added to the nerve cell culture. | 05-19-2016 |
20160137962 | CULTURE CHAMBER AND CULTURE METHOD - A culture chamber includes a plurality of recesses ( | 05-19-2016 |
20160137983 | Synthetic Surfaces For Culturing Stem Cell Derived Cardiomyocytes - Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media. | 05-19-2016 |
20160145570 | METHODS FOR DIFFERENTIATING CELLS INTO HEPATIC STELLATE CELLS AND HEPATIC SINUSOIDAL ENDOTHELIAL CELLS, CELLS PRODUCED BY THE METHOD, AND METHODS FOR USING THE CELLS - The invention is directed to methods for culturing cells so that the cells are induced to differentiate into cells that express a hepatic stellate phenotype and cells that express a hepatic sinusoidal endothelial phenotype. The invention is also directed to cells produced by the methods of the invention. The cells are useful, among other things, for treatment of liver deficiency, liver metabolism studies, and liver toxicity studies. | 05-26-2016 |
20160145574 | KIT AND METHOD FOR PROMOTING MESENCHYMAL STEM CELL DIFFERENTIATION - The present invention is related to a kit comprising: (a) a mesenchymal stem cell; (b) a gelatin-hyaluronan-chondroitin tri-copolymer scaffold; (c) a kartogenin; and (d) a bioreactor. The present invention is also related to a method for promoting differentiation of a mesenchymal stem cell into cartilage tissue, comprising: (a) culturing the mesenchymal stem cell on a gelatin- hyaluronan-chondroitin tri-copolymer scaffold in the presence of a kartogenin; and (b) culturing the mesenchymal stem cell and the gelatin-hyaluronan-chondroitin tri-copolymer scaffold in a bioreactor. | 05-26-2016 |
20160168525 | Cell Culturing Method, Particulate Culture Carrier, and Particle-Encompassing Cell Aggregate | 06-16-2016 |
20160168535 | GENERATION OF ANTERIOR FOREGUT ENDODERM FROM PLURIPOTENT CELLS | 06-16-2016 |
20160168536 | HEPATIC CELL LINES AND STEM-LIKE CELLS, METHODS OF MAKING AND USING THE SAME | 06-16-2016 |
20160168537 | DIFFERENTIATED CELLULAR COMPOSITIONS AND METHODS FOR THEIR PREPARATION AND USE | 06-16-2016 |
20160168541 | TRANSDUCIBLE MATERIALS FOR CELL REPROGRAMMING | 06-16-2016 |
20160175799 | CROSS-LINKED CARBON NANOTUBE NETWORKS | 06-23-2016 |
20160177244 | AUTOMATED CELL CULTURE SYSTEM AND METHOD | 06-23-2016 |
20160177265 | METHOD FOR PRODUCING MEGAKARYOCYTES, PLATELETS AND/OR THROMBOPOIETIN USING MESENCHYMAL CELLS | 06-23-2016 |
20160177266 | MUSCLE STEM CELL IN VITRO CULTURE METHOD AND APPLICATION | 06-23-2016 |
20160177267 | SERUM-FREE IN VITRO DIRECTED DIFFERENTIATION PROTOCOL FOR GENERATING STEM CELL-DERIVED BETA CELLS AND USES THEREOF | 06-23-2016 |
20160194602 | Induction Medium and Methods for Stem Cell Culture and Therapy | 07-07-2016 |
20160194606 | Methods for Inducing the Differentiation of Blood Monocytes into Functional Dendritic Cells | 07-07-2016 |
20160194607 | Cell Culture Medium for the Growth and Differentiation of Cells of the Hematopoietic Lineage | 07-07-2016 |
20160251612 | CULTURE MEDIUM FOR ESTABLISHING NEUROEPITHELIAL STEM CELLS, METHOD FOR ESTABLISHING NEUROEPITHELIAL STEM CELLS, AND APPLICATIONS THEREOF | 09-01-2016 |
20160376559 | NUCLEAR RECEPTOR AND MUTANT THEREOF AND THE USE OF THE SAME IN THE REPROGRAMMING OF CELLS - According to the invention there is provided methods for inducing pluripotent stem cells in vitro, vectors and compositions for producing the same and methods for using the induced pluripotent stem cell for treating a patient in need of a pluripotent stem cell treatment. | 12-29-2016 |
20190142867 | METHOD FOR GENERATING PROGENITOR T CELLS FROM STEM AND/OR PROGENITOR CELLS AND USE OF SAME | 05-16-2019 |
20190144824 | STANDARDIZED NEURONAL CELL ASSAYS FROM PRIMATE SPECIES | 05-16-2019 |