Entries |
Document | Title | Date |
20080220502 | Directed evolution of microorganisms - The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods. | 09-11-2008 |
20080299644 | E.Coli Mutant Containing Mutant Genes Related with Tryptophan Biosynthesis and Production Method of Tryptophan by Using the Same - The present invention relates to a Tryptophan-producing | 12-04-2008 |
20090004724 | METHOD FOR ENHANCING PRODUCTION OF ISOPRENOID COMPOUNDS - The present invention provides methods of producing an isoprenoid or an isoprenoid precursor in a genetically modified host cell. The methods generally involve modulating the level of hydroxymethylglutaryl-CoA (HMG-CoA) in the cell, such that the level of HMG-CoA is not toxic to the cell and/or does not substantially inhibit cell growth, but is maintained at a level that provides for high-level production of mevalonate, IPP, and other downstream products of an isoprenoid or isoprenoid pathway, e.g., polyprenyl diphosphates and isoprenoid compounds. The present invention further provides genetically modified host cells that are suitable for use in a subject method. The present invention further provides recombinant nucleic acid constructs for use in generating a subject genetically modified host cell, including recombinant nucleic acid constructs comprising nucleotide sequences encoding one or more mevalonate pathway enzymes, and recombinant vectors (e.g., recombinant expression vectors) comprising same. The present invention further provides methods for identifying nucleic acids that encode HMG-CoA reductase (HMGR) variants that provide for relief of HMG-CoA accumulation-induced toxicity. The present invention farther provides methods for identifying agents that reduce intracellular accumulation of HMG-CoA. | 01-01-2009 |
20090023198 | OPTIMIZING EXPRESSION OF ACTIVE BOTULINUM TOXIN TYPE A - Nucleic acid molecules that comprise modified open reading frames providing increased expression of the encoded active BoNT/A in a heterologous cell, expression constructs and cells comprising such nucleic acid molecules and methods useful for expressing the encoding active BoNT/A from such nucleic acid molecules, expression constructs and cells. | 01-22-2009 |
20090029444 | NOVEL RECOMBINANT ANTICOAGULANT PROTEINS - Novel recombinant anticoagulation proteins, methods of their use and methods of their production are described. In particular, recombinant fusions of annexin V (ANV) and Kunitz protease inhibitors (KPI) that possess potent anticoagulant activity are provided. The fusions, abbreviated ANV:KPI, utilize ANV having high affinity for phosphatidyl-L-serine with various KPI's to target serine proteases in membrane-associated coagulation complexes in the blood coagulation cascade. ANV:KPIs are potentially useful antithrombotic drugs permitting localized passivation of thrombogenic vessel walls and associated thrombi. | 01-29-2009 |
20090042275 | NOVEL PLASMIDS AND UTILIZATION THEREOF - A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genus | 02-12-2009 |
20090053795 | RECOMBINANT CANDIDA RUGOSA LIPASES - The present invention features an isolated nucleic acid that includes a mutant DNA encoding a | 02-26-2009 |
20090068723 | Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus - The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus. | 03-12-2009 |
20090093043 | THERMOSTABLE NUCLEIC ACID POLYMERASE FROM THERMOCOCCUS GORGONARIUS - A purified thermostable enzyme is derived form the thermophilic archaebacterium | 04-09-2009 |
20090104683 | L-TYROSINE-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING L-TYROSINE - The present invention describes the production of L-tyrosine by culturing in a medium an | 04-23-2009 |
20090130741 | De novo synthesized plasmid, methods of making and use thereof - The invention relates to a de novo synthesized plasmid. The plasmid comprises relevant sequences for plasmid replication and plasmid selection. The methods of making and use of the plasmid are disclosed. The plasmid can be used to make other plasmids. These plasmids and their host cells can be used for biomedical applications. | 05-21-2009 |
20090155888 | FLUORESCENT PROTEIN - The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); | 06-18-2009 |
20090191611 | Methods and Compositions for the Treatment of Gastrointestinal Disorders - The present invention features compositions and related methods for treating IBS and other gastrointestinal disorders and conditions (e.g., gastrointestinal motility disorders, functional gastrointestinal disorders, gastroesophageal reflux disease (GERD), duodenogastric reflux, Crohn's disease, ulcerative colitis, Inflammatory bowel disease, functional heartburn, dyspepsia (including functional dyspepsia or nonulcer dyspepsia), gastroparesis, chronic intestinal pseudo-obstruction (or colonic pseudo-obstruction), and disorders and conditions associated with constipation, e.g., constipation associated with use of opiate pain killers, post-surgical constipation (post-operative ileus), and constipation associated with neuropathic disorders as well as other conditions and disorders using peptides and other agents that activate the guanylate cyclase C (GC-C) receptor. | 07-30-2009 |
20090197321 | STRAIN OF GENETICALLY REENGINEERED ESCHERICHIA COLI FOR BIOSYNTHESIS OF HIGH YIELD CAROTENOIDS AFTER MUTATION SCREENING - The present invention relates to a strain of | 08-06-2009 |
20090215154 | Method of preparing micro-and nanometric particles with labile Products - The invention relates to a method of obtaining micro- and nanometric polymeric particles in a controlled, reproducible manner. The aforementioned particles have a spherical shape and a very narrow, uniform size distribution. The invention comprises the use of an easy particle-forming method consisting in using hydrodynamic forces to focus a composite microjet formed by two concentric fluids and can be used in the encapsulation of fragile compounds of biological interest, from peptides and proteins to cells and micro-organisms. | 08-27-2009 |
20090258409 | PURIFIED SR-P70 PROTEIN - The invention relates to new nucleic acid sequences of the family of tumor-suppressing genes related to the gene for the p53 protein, and to corresponding protein sequences. | 10-15-2009 |
20090258410 | METHODS FOR PRODUCING SOLUBLE MEMBRANE SPANNING PROTEINS - Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts. | 10-15-2009 |
20090280556 | Mesothelin, A Differentiation Antigen Present On Mesothelium, Mesotheliomas, and Ovarian Cancers and Methods and Kits for Targeting the Antigen - This invention relates to the discovery of a differentiation antigen termed mesothelin which is associated with mesotheliomas and ovarian cancers. Mesothelin is about 69 kD in its full-length form. The invention includes uses for the amino acid and nucleic acid sequences for mesothelin, recombinant cells expressing it, methods for targeting and/or inhibiting the growth of cells bearing mesothelin, methods for detecting the antigen and its expression level as an indication of the presence of tumor cells, and kits for such detection. | 11-12-2009 |
20100062514 | REARRANGED SQUAMOUS CELL CARCINOMA ANTIGEN GENES II - The present invention relates to a SCCA1/SCCA2 fusion protein; plasmid containing the same; antibodies of said fusion protein; methods for detecting said protein; methods for diagnosing the presence or absence of SCC by determining the presence of SCCA1/SCCA2 fusion protein. | 03-11-2010 |
20100075401 | MINICIRCLE DNA VECTOR PREPARATIONS AND METHODS OF MAKING AND USING THE SAME - The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. | 03-25-2010 |
20100086989 | MUTANT GLUCOSE DEHYDROGENASE - A mutant glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 3 or an amino acid sequence of SEQ ID NO: 3 including substitution, deletion, insertion or addition of one or more amino acid residues other than the amino acid residue at the 365th position and having glucose dehydrogenase activity, wherein an amino acid residue at a position corresponding to the 365th position of, the amino acid sequence is replaced with another amino acid residue, and the mutant glucose dehydrogenase shows an improved substrate specificity to glucose. | 04-08-2010 |
20100099168 | Artificial antibody polypeptides - A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, k | 04-22-2010 |
20100099169 | GENETIC SELECTION SYSTEM FOR IMPROVING RECOMBINANT PROTEIN EXPRESSION - A method for selecting host cells with an improved ability to recombinantly overexpress a target protein; the host cells thus generated and their use. The invention also provides a curing method to remove plasmids from host cell lines. | 04-22-2010 |
20100112672 | PRODUCTION OF ISOPRENOIDS AND ISOPRENOID PRECURSORS - The present invention provides genetically modified host cells and use of same for producing isoprenoid compounds. | 05-06-2010 |
20100112673 | DNA SEQUENCE ENCODING PENICILLIN ACYLASE, NOVEL RECOMBINANT DNA CONSTRUCTS AND RECOMBINANT MICROORGANISMS CARRYING THIS SEQUENCE - The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from | 05-06-2010 |
20100112674 | MICROCIN H47 PLASMID SELECTION SYSTEM - The present invention relates generally to stabilized expression plasmid systems. The stabilized expression plasmid systems comprise an expression vector that includes a plasmid maintenance system (PMS) and, optionally, one or both of a polynucleotide encoding a selected antigen under control of a promoter, and a polynucleotide encoding a selectable marker under control of a promoter. The use of the mchI protein as a selectable marker is found in preferred embodiments of the invention. | 05-06-2010 |
20100136663 | PREPARATION OF AN ARTIFICIAL TRANSCRIPTION FACTOR COMPRISING ZINC FINGER PROTEIN AND TRANSCRIPTION FACTOR OF PROKARYOTE, AND A USE THEREOF - The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an | 06-03-2010 |
20100173389 | PROCESS FOR BACTERIAL PRODUCTION OF POLYPEPTIDES - Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so as to release refractile particles from the bacterial cellular matrix, and recovering the released refractile particles from the periplasm. Chloroform is not used in any step and the recovery step minimizes co-recovery of cellular debris with the released refractile particles. | 07-08-2010 |
20100184195 | Recycling System for Manipulation of Intracellular NADH Availability - The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments. | 07-22-2010 |
20100255561 | HOST-VECTOR SYSTEM FOR CLONING AND EXPRESSING GENES - A system for ligase-free cloning and/or expressing a target gene is described herein. A preferred version of the invention includes an | 10-07-2010 |
20100261258 | MODIFIED BACTERIOCINS AND METHODS FOR THEIR USE - Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of | 10-14-2010 |
20100267121 | RECOMBINANT VECTOR CONTAINING INFECTIOUS HUMAN CYTOMEGALOVIRUS GENOME WITH PRESERVED WILD-TYPE CHARACTERISTICS OF CLINICAL ISOLATES - A recombinant vector containing infectious genome of human cytomegalovirus (HCMV) and being useful for the production of reconstituted HCMV virus retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells and to induce microfusion is characterized in that it is obtainable by inserting DNA from a clinical isolate of HCMV virus into a bacterial cloning vehicle. Such vector can be used e.g., for production of reconstituted HCMV virus retaining the phenotypic characteristics of a parental clinical isolate and for studying genes and functions of genes of HCMV virus. A further aspect are mutant viruses and inter alia their use for studying aspects of infectivity of HCMV virus. | 10-21-2010 |
20100273240 | METHOD FOR PRODUCTION AND PURIFICATION OF MACROMOLECULAR COMPLEXES - The present invention relates to a method for production and purification of affinity tagged macromolecular complexes, such as ribosomes. More closely, the method comprises in-frame fusion of a nucleotide sequence specific for an affinity tag and a selection marker, wherein the fusion is at the chromosomal site of a gene encoding a multicopy protein, and wherein the macromolecular complex is expressed with multiple copies of said affinity tag. The invention also relates to affinity tagged ribosomes, to cells comprising such affinity tagged ribosomes, and to various uses thereof. | 10-28-2010 |
20100311147 | ALLELES OF THE REL GENE FROM CORYNEFORM BACTERIA - An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described. | 12-09-2010 |
20100317086 | LARGE SCALE MICROBIAL CULTURE METHOD - A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up. | 12-16-2010 |
20100330657 | Activin receptor-like kinases, proteins having serine threonine kinase domains and their use - A new receptor family has been identified, of activin-like kinases. Novel proteins have activin/TGF-β-type I receptor functionality, and have consequential diagnostic/therapeutic utility. They may have a serine/threonine kinase domain, a DFKSRN or DLKSKN sequence in subdomain VIB and/or a GTKRYM sequence in subdomain VIII. | 12-30-2010 |
20110020911 | SOLUBILIZATION AND PURIFICATION OF A TARGET PROTEIN FUSED TO A MUTANT MALTOSE-BINDING PROTEIN - Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein. | 01-27-2011 |
20110027865 | EVERNINOMICIN BIOSYNTHETIC GENES - This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel eveminomicin-related compounds based on eveminomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A | 02-03-2011 |
20110045575 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. | 02-24-2011 |
20110053252 | MICROORGANISM PRODUCING O-ACETYL-HOMOSERINE AND THE METHOD OF PRODUCING O-ACETYL-HOMOSERINE USING THE MICROORGANISM - Disclosed herein are a microorganism strain capable of producing the L-methionine precursor O-acetyl homoserine in high yield and a method of producing O-acetyl homoserine using the same. The microorganism strain is a strain of | 03-03-2011 |
20110053253 | MICROORGANISM PRODUCING O-ACETYL-HOMOSERINE AND THE METHOD OF PRODUCING O-ACETYL-HOMOSERINE USING THE MICROORGANISM - Disclosed is a strain of | 03-03-2011 |
20110053254 | MATERIALS AND METHODS RELATING TO MODIFYING THE BINDING OF ANTIBODIES - The invention relates to materials and methods for modifying the binding of antibodies, and more particularly to antibodies that are obtainable by inserting an amino acid sequence capable of binding to a target into a complementarity determining region of a parent antibody so that the antibody thus obtained is capable of binding to the target. The invention further relates to the uses of the antibodies for therapy, diagnosis or imaging, and to methods of producing the antibodies. | 03-03-2011 |
20110070626 | Nucleic acid and amino acid sequences relating to Staphylococcus epidermidis for diagnostics and therapeutics - The invention provides isolated polypeptide and nucleic acid sequences derived from | 03-24-2011 |
20110086416 | IMMUNOTOXIN FUSION PROTEINS AND MEANS FOR EXPRESSION THEREOF - The present invention described and shown in the specification and drawings provides novel recombinant DT-based immunotoxins, and, more specifically anti-T cell immunotoxin fusion proteins. Also provided are immunotoxins that can be expressed in bacterial, yeast, or mammalian cells. The invention also provides means for expression of the immunotoxin fusion protein. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims. | 04-14-2011 |
20110111483 | Optimizing Expression of Active Botulinum Toxin Type E - Nucleic acid molecules that comprise modified open reading frames providing increased expression of the encoded active BoNT/E in a heterologous cell, expression constructs and cells comprising such nucleic acid molecules and methods useful for expressing the encoding active BoNT/E from such nucleic acid molecules, expression constructs and cells. | 05-12-2011 |
20110124090 | GENE INVOLVED IN THE BIOSYNTHESES OF LYCOPENE, RECOMBINANT VECTOR COMPRISING THE GENE, AND TRANSFORMED MICROORGANISM WITH THE RECOMBINANT VECTOR - There are provided genes involved in the biosynthesis of lycopene and having DNA sequences set forth in SEQ ID NO: 1, SEQ ID NO: 3 and SEQ ID NO: 5 encoding proteins required for the biosynthesis of lycopene, a recombinant vector comprising at least one of the genes, and a mi | 05-26-2011 |
20110129904 | METHODS AND ORGANISMS FOR CONVERTING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL TO 1,3-BUTANEDIOL - A non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway includes at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme or protein expressed in a sufficient amount to produce 1,3-BDO. A method for producing 1,3-BDO that includes culturing the this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO. | 06-02-2011 |
20110165659 | BIOLOGICAL PRODUCTS - There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human CD22. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a modified CDR. Further disclosed are DNA sequences encoding the claims of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by cells expressing CD22. | 07-07-2011 |
20110212508 | Novel Synthetic Expression Vehicle - An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from | 09-01-2011 |
20110244553 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 10-06-2011 |
20110269215 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 11-03-2011 |
20110269216 | EVERNINOMICIN BIOSYNTHETIC GENES - This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A | 11-03-2011 |
20110287521 | BIOSYNTHESIS OF COMMODITY CHEMICALS - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 11-24-2011 |
20120040441 | TRANSFORMED STRAINS ORIGINATED FROM MULTIDRUG EFFLUX PROTEIN DEFECTIVE STRAINS AND A METHOD FOR MICROBIAL CONVERSION USING THEM - Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion system using a transformant which is given by introducing a gene originated from xerogenic organisms. A transformant is prepared by using a host which is defective in a gene encoding a multidrug efflux protein and introducing a gene originated from xerogenic organisms. Use of the transformant results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound. In case, an | 02-16-2012 |
20120088289 | VMP-LIKE SEQUENCES OF PATHOGENIC BORRELIA SPECIES AND STRAINS - The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic | 04-12-2012 |
20120115208 | MODULAR METHOD FOR RAPID ASSEMBLY OF DNA - The invention is directed to methods, kits and compositions using specially designed nucleic acid components for efficient assembly of a DNA construct. The method involves a) incubating a support with a first form of nucleic acid components under conditions to form support-bound nucleic acid component complexes; b) removing unbound first form nucleic acid components; c) incubating the support-bound first form nucleic acid component complexes with a second form of nucleic acid components under conditions to anneal and link the second form to the first form; d) removing unbound second form nucleic acid components; e) repeating steps c) and d) until the DNA construct is generated; and f) eluting the DNA construct from the support. The first and second forms of the nucleic acid component comprise sticky ends such that each form cannot link to itself but can link to each other to form an alternating head to tail sequence. | 05-10-2012 |
20120142081 | BIOSYNTHESIS OF COMMODITY CHEMICALS - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 06-07-2012 |
20120149090 | CELL FOR PREPARING COMPETENT CELL, METHOD FOR PREPARING COMPETENT CELL AND BACTERIAL STRAIN OF ESCHERICHIA COLI - The invention provides a cell for preparing competent cells, wherein the cell is capable of spontaneously accumulating self-producing trehalose therein and the cell is used for the preparation of competent cells. The invention also provides a method for preparing competent cells, including: culturing the cell for preparing the competent cell mentioned previously to obtain a cell suspension; placing the cell suspension into an ice bath; centrifuging the cell suspension to obtain a cell precipitate; mixing a transform reagent with the cell precipitate; and obtaining competent cells suspension. | 06-14-2012 |
20120196353 | LIGF-TYPE SYSTEMS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-02-2012 |
20120202272 | LIGE-TYPE SYSTEMS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-09-2012 |
20120270301 | PROTEINS WITH REPETITIVE BACTERIAL-IG-LIKE (BIG) DOMAINS PRESENT IN LEPTOSPIRA SPECIES - The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the | 10-25-2012 |
20130023036 | NUCLEIC ACID CONSTRUCT, RECOMBINANT VECTOR, AND RECOMBINANT E. COLI PRODUCING CHICKEN ANEMIA VIRUS VP1 PROTEIN - Disclosed herein is an expression cassette adapted to be expressed in an | 01-24-2013 |
20130034897 | BACTERIAL HOST CELL FOR THE DIRECT EXPRESSION OF PEPTIDES - Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed. | 02-07-2013 |
20130102057 | VMP-LIKE SEQUENCES OF PATHOGENIC BORRELIA SPECIES AND STRAINS - The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic | 04-25-2013 |
20130143304 | LIGF-TYPE SYSTEMS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 06-06-2013 |
20130149772 | MAGNETIC ION-EXCHANGE RESIN AND METHOD FOR THE PREPARATION THEREOF - Magnetic ion-exchange polymer microspheres and a method for preparing the same are provided. The method for preparing the magnetic ion-exchange polymer microspheres includes swelling the ion-exchange resins and allowing the magnetic nano-particles to enter the interior of the ion-exchange resins. The magnetic ion-exchange resins of the present invention have various functional groups can be introduced onto the surfaces thereof. Therefore, the magnetic ion-exchange resins of the present invention can be applied in many areas, and thereby they have high economic value. | 06-13-2013 |
20130164823 | Expression System - An immunogenic reagent which produces an immune response which is protective against | 06-27-2013 |
20130210120 | Inducible Gene Expression Composition for Using Eukaryotic Pol-2 Promoter-Driven Transcription in Prokaryotes and the Applications Thereof - Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells. The RNAs and peptides/proteins so obtained can be used to develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, and make foods. | 08-15-2013 |
20130230903 | PROTEINS WITH REPETITIVE BACTERIAL-IG-LIKE (BIG) DOMAINS PRESENT IN LEPTOSPIRA SPECIES - The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the | 09-05-2013 |
20130252312 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 09-26-2013 |
20130267011 | Method For Rapidly Developing Gene Switches And Gene Circuits - Provided are: a selection method for a gene switch and a gene circuit, including using, as a selector, an expression vector containing at least a gene sequence whose expression is controlled by a transcription regulatory factor to be expressed when a genetic switch and a genetic circuit including the genetic switch operate, and a promoter sequence operably linked to the gene sequence upstream thereof; and an expression vector to be used in the selection method. This enables an effective selection method for a genetic switch and a genetic circuit, the selection method being able to be conducted within a short time period and with high selection efficiency and less leakiness. | 10-10-2013 |
20130323821 | Non-Natural Amino Acid Replication-Dependent Microorganisms and Vaccines - Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids. | 12-05-2013 |
20130337547 | THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS - A recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from | 12-19-2013 |
20140004597 | SYNTHETIC PATHWAYS FOR BIOFUEL SYNTHESIS | 01-02-2014 |
20140011261 | NITROGEN FIXATION GENE ISLAND SUITABLE FOR EXPRESSING IN PROKARYOTIC AND EUKARYOTIC SYSTEMS - Provided is a nitrogen fixation gene island suitable for expressing in prokaryotic and eukaryotic systems, wherein the prokaryotic nitrogen fixation genes are modified using T7 promoters to make them suitable for eukaryotic expression. | 01-09-2014 |
20140038267 | NOVEL MONOMERIC YELLOW-GREEN FLUORESCENT PROTEIN FROM CEPHALOCHORDATE - The present disclosure provides isolated nucleic acid sequences encoding a monomeric green/yellow fluorescent proteins, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present invention further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present invention provides a monomeric green/yellow fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the green/yellow fluorescent protein. | 02-06-2014 |
20140045247 | FUSION PROTEIN - The present invention provides means useful for making devices, materials and the like that are excellent in photocatalytic activity, electric property or the like. Specifically, the present invention provides a fusion protein comprising a polypeptide portion capable of forming a multimer having an internal cavity, and a first peptide portion capable of binding to a first target substance and a second peptide portion capable of binding to a second target substance; a multimer of the fusion protein; a complex comprising the multimer of the fusion protein; and the like. | 02-13-2014 |
20140065697 | CELLS AND METHODS FOR PRODUCING ISOBUTYRIC ACID - Disclosed herein are cells and methods for renewably producing isobutyrate. In some cases, the cells can include a heterologous DNA that encodes at least one enzyme that catalyzes the conversion of isobutyraldehyde to isobutyrate. In other cases, the cells can include a genetically modified enzyme that catalyzes the conversion of isobutyraldehyde to isobutyrate to a degree greater than the wild-type version of the enzyme. In other cases, the cells can include one or more enzyme that catalyze the conversion of 2-ketovaline to isobutyrate. Generally, methods include growing the cells in a medium that includes a carbon source that the cells are able to convert to isobutyrate. | 03-06-2014 |
20140065698 | Recombinant microorganisms and uses therefor - A stereospecific enzyme in | 03-06-2014 |
20140065699 | NOVEL BACTERIAL EXPRESSION PLASMID - The present invention provides expression vectors useful for high-throughput screening of gene libraries. In a specific embodiment, an expression vector comprising (a) the Rop gene operatively linked to the trp promoter-operator; (b) a purification tag sequence and a protease cleavage site downstream of the Rop gene; and (d) a multiple cloning site downstream of the protease cleavage site, wherein the insertion of a heterologous gene of interest into the multiple cloning site and subsequent expression thereof in a host cell produces a high yield of a fusion protein comprising the Rop protein and the protein encoded by the heterologous gene of interest without the need of chemical inducers, temperature shifts, or growth medium alterations to initiate protein synthesis, and wherein the fusion protein controls plasmid replication at temperatures below about 30° C. but exhibits runaway plasmid replication when cultured at about 37° C. | 03-06-2014 |
20140080201 | Biosynthesis of Human Milk Oligosaccharides in Engineered Bacteria - The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection. | 03-20-2014 |
20140087447 | Cell Modeling of Heme Deficiency using Ferrochelatase Mutations - Provided herein, is a means for development of ferrochelatase variants with improved tolerance towards N-methyl protoporphyrin. Also disclosed are cell assay systems utilizing the variants, as the variants would confer resistance to N-methyl protoporphyrin inhibition and thereby keep heme synthesis uninterrupted. The variants contain loop mutations that affect the NMPP-ferrochelatase interaction, and different degrees of NMPP tolerance are obtained with the introduction of loop mutations in wild-type ferrochelatase. Also disclosed is kinetic mechanism of inhibition of ferrochelatase by NMPP, using the disclosed variants whose mutations in the “porphyrin-interacting loop” motif weakened the potency of NMPP as an inhibitor. | 03-27-2014 |
20140093942 | GENE, ARS-R ANCHORAGE CASSETTE, ARS-R EXPRESSION-ANCHORAGE CASSETTE, RECOMBINANT PLASMID, BACTERIAL TRANSGENIC LINEAGE, USE OF SAID GENE, USE OF SAID LINEAGE IN ENVIRONMENTAL BIOREMEDIATION PROCESSES - The present invention relates to the construction and insertion of a DNA plasmid vector of broad spectrum for Gram-Negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelator protein for arsenic ions on the Gram-Negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsR gene without stop codon (SEQ ID No 1) was amplified by Polymerase Chain Reaction (PCR) using as a template the chromosome 1 of | 04-03-2014 |
20140099699 | NON-NATURALLY OCCURRING T CELL RECEPTORS - A T cell receptor (TCR) having the property of binding to the gp100 YLEPGPVTA peptide-HLA-A2 complex and comprising a TCR alpha variable domain and/or a TCR beta variable domain, characterized in that the domains are mutated relative to a TCR having the extracellular alpha and beta chain sequences SEQ ID NOs: 2 and that the TCR has a binding affinity for, and/or a binding half-life for, the YLEPGPVTA-HLA-A2 complex at least double that of a reference TCR. Embodiments of the invention such as the use of such TCRs in adoptive therapy, and fusions of such TCRs with therapeutic agents are also described. | 04-10-2014 |
20140147909 | Method for Producing an L-Amino Acid Using a Bacterium of the Enterobacteriaceae Family - The present invention provides a method for producing an L-amino acid from ethanol using a bacterium of the | 05-29-2014 |
20140193885 | PRODUCTION OF ITACONIC ACID - The invention relates to a nucleic acid sequence encoding an | 07-10-2014 |
20140199754 | PRODUCTION OF ITACONIC ACID - The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of | 07-17-2014 |
20140212951 | EXPRESSION CONDITIONS AND METHODS OF HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) - A cell culture media for growing transformant pGDF-5-Trc-transformed cells is provided for increased production of transformant pGDF-5-Trc-transformed cells. Also provided herein are methods of growing the transformant pGDF-5-Trc-transformed cells. The methods of growing the transformant pGDF-5-Trc-transformed cells as disclosed are cost-effective, time-saving and are of manufacturing quality. | 07-31-2014 |
20140212952 | METHODS AND COMPOSITIONS OF HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) ISOLATED FROM INCLUSION BODIES - Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for isolating recombinant GDF-5 protein from an inclusion body of a cell are disclosed herein. The methods as disclosed are cost-effective, time-saving and are of manufacturing quality. | 07-31-2014 |
20140248688 | PLANT WALL DEGRADATIVE COMPOUNDS AND SYSTEMS - The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications. | 09-04-2014 |
20140256020 | IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity. | 09-11-2014 |
20140273162 | METABOLICALLY ENGINEERED ESCHERICHIA COLI FOR ENHANCED PRODUCTION OF SIALIC ACID - A metabolically engineered | 09-18-2014 |
20140273163 | OLIGOSACCHARIDE COMPOSITIONS, GLYCOPROTEINS AND METHODS TO PRODUCE THE SAME IN PROKARYOTES - Disclosed are methods and compositions to produce various oligosaccharide compositions and glycoproteins. Prokaryotic hosts cells are cultured under conditions effective to produce human-like e.g., high-mannose, hybrid and complex glycosylation patterns by introducing glycosylation pathways into the host cells. | 09-18-2014 |
20140273164 | NON-CO2 EVOLVING METABOLIC PATHWAY FOR CHEMICAL PRODUCTION - Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a suitable carbon source. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms. | 09-18-2014 |
20140273165 | RECOMBINANT MICROORGANISMS HAVING A METHANOL ELONGATION CYCLE (MEC) - Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms. | 09-18-2014 |
20140287484 | ENGINEERED ENZYMES WITH METHIONINE-GAMMA-LYASE ENZYMES AND PHARMACOLOGICAL PREPARATIONS THEREOF - Methods and composition related to the engineering of a novel protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids. | 09-25-2014 |
20140308734 | PROTEINS WITH REPETITIVE BACTERIAL-IG-LIKE (BIG) DOMAINS PRESENT IN LEPTOSPIRA SPECIES - The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the | 10-16-2014 |
20140349376 | BETA-ALANINE/ALPHA-KETOGLUTARATE AMINOTRANSFERASE FOR 3-HYDROXYPROPIONIC ACID PRODUCTION - The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof. | 11-27-2014 |
20150024468 | METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL - The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO | 01-22-2015 |
20150024469 | ENDOLYSIN OBPGPLYS - The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent. The present invention also relates to the use of said polypeptide or fusion protein for the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to a pharmaceutical composition comprising said polypeptide or fusion protein. | 01-22-2015 |
20150044755 | PRODUCTION OF MUCONIC ACID FROM GENETICALLY ENGINEERED MICROORGANISMS - This present invention is in the field of producing renewable chemical feedstocks using biocatalysts that have been genetically engineered to increase their ability to convert renewable carbon resources into useful compounds. More specifically, the present invention provides a process for producing muconic acid form renewable carbon resources using a genetically modified organism. | 02-12-2015 |
20150056684 | METHODS, SYSTEMS, AND COMPOSITIONS FOR INCREASED MICROORGANISM TOLERANCE TO AND PRODUCTION OF 3-HYDROXYPROPIONIC ACID (3-HP) - The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex (“3HPTGC”). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made to provide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC. | 02-26-2015 |
20150064771 | INDUCIBLE GENE EXPRESSION COMPOSITION FOR USING EUKARYOTIC POL-2 PROMOTER-DRIVEN TRANSCRIPTION IN PROKARYOTES AND THE APPLICATIONS THEREOF - Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells. The RNAs and peptides/proteins so obtained can be used to develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, and make foods. | 03-05-2015 |
20150104853 | COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE - Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity. | 04-16-2015 |
20150118734 | INDUCIBLE GENE EXPRESSION COMPOSITION FOR USING EUKARYOTIC POL-2 PROMOTER-DRIVEN TRANSCRIPTION IN PROKARYOTES AND THE APPLICATIONS THEREOF - Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells. The RNAs and peptides/proteins so obtained can be used to develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, and make foods. | 04-30-2015 |
20150125933 | PRODUCTION OF FATTY ACIDS AND DERIVATIVES THEREOF HAVING IMPROVED ALIPHATIC CHAIN LENGTH AND SATURATION CHARACTERISTICS - The invention relates to compositions, including polynucleotide sequences, amino acid sequences, recombinant microorganisms, and recombinant microorganism cultures that produce compositions of fatty acids and derivatives having target aliphatic chain lengths and/or preferred percent saturation. Further, the invention relates to methods of making and using the compositions. The compositions and methods provide for high titers, high yields, and high productivities of fatty acids and derivatives thereof. | 05-07-2015 |
20150125934 | METHOD FOR SELECTIVELY CULTURING MICROORGANISM USING PHOSPHITE DEHYDROGENASE GENE AS MARKER - A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced. | 05-07-2015 |
20150132828 | BACTERIAL HOST STRAIN COMPRISING A MUTANT SPR GENE AND HAVING REDUCED TSP ACTIVITY - The present invention provides a recombinant gram-negative bacterial cell comprising a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and wherein the cell has reduced Tsp protein activity compared to a wild-type cell. | 05-14-2015 |
20150337320 | Engineered Light-Harvesting Organisms - The present disclosure identifies pathways and mechanisms to confer photoautotrophic properties to a heterotrophic organism. The resultant engineered cell or organism will uniquely enable efficient conversion of carbon dioxide and light into biomass and carbon-based products of interest. | 11-26-2015 |
20150344840 | BACTERIAL HOST STRAIN - A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest. | 12-03-2015 |
20150361435 | IDENTIFICATION OF INHIBITORS OF A BACTERIAL STRESS RESPONSE - A system, a composition, a method and a kit for identifying anti-bacterial agents are provided. The invention described herein is useful in identifying inhibitors of any bacterial stress response. Moreover, the invention can be applied to any sRNA and its target, any transcription factor and its target, and any transcription factor/sRNA pair (i.e., a transcription factor that regulates a sRNA). In particular, the present invention provides a system, a composition, a method and a kit for the identification of cyclic peptides that block the σ | 12-17-2015 |
20150376656 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 12-31-2015 |
20160115492 | MICROORGANISMS ENGINEERED TO USE UNCONVENTIONAL SOURCES OF NITROGEN - Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals. | 04-28-2016 |
20160122407 | Galectin-3 Inhibitor (Gal-3M) is Associated with Additive Anti-Myeloma and Anti-Solid Tumor Effects, Decreased Osteoclastogenesis and Organ Protection when Used in Combination with Proteasome Inhibitors - The present invention includes a method for the treatment of myeloma comprising identifying a patient with myeloma and administering to the patient a synergistic, effective amount of a truncated, dominant negative form of Galectin-3 and a proteasome inhibitor. | 05-05-2016 |
20160159867 | NUCLEOTIDE SEQUENCES, VECTORS AND HOST CELLS - Polynucleotides encoding fusion proteins comprising fragments of toxin A and toxin B from | 06-09-2016 |
20160186189 | SITE-SPECIFIC INCORPORATION OF PHOSPHOSERINE INTO PROTEINS IN ESCHERICHIA COLI - Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNA | 06-30-2016 |
20170233713 | HYDROCARBON SYNTHASE GENE AND USE THEREOF | 08-17-2017 |