03rd week of 2015 patent applcation highlights part 47 |
Patent application number | Title | Published |
20150017641 | Mechanical Washing and Measuring Device for Performing Analyses - The invention describes simple and reliable cartridge constructions for carrying out analytical procedures in a closed system, especially for carrying out bioaffinity analyses. The cartridge exploits a measuring component, or a test strip ( | 2015-01-15 |
20150017642 | METHOD FOR THE DIAGNOSIS OF MYELOID NEOPLASIAS AND SOLID TUMORS AND DIAGNOSTIC KITS - The present application relates to an ex vivo or in vitro method to determine the presence or absence of a mutation or a variation in the NF-E2 gene in a sample from a patient suffering from a myeloid neoplasm or solid tumor whereby said method comprises: | 2015-01-15 |
20150017643 | METHOD OF MOLECULAR TYPING - The invention relates to the identification of genetic traits in an individual. More specifically, the invention relates to identifying human leukocyte antigen (HLA) alleles and haplotypes in a potential donor sample. The invention can be used for matching a donor with a recipient for transplantation, other medical needs and high-through put screening of samples. | 2015-01-15 |
20150017644 | PLANT TRANSFORMATION WITHOUT SELECTION - The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided. | 2015-01-15 |
20150017645 | Qualitative and Quantitative Detection of Microbial Nucleic Acids - The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses. | 2015-01-15 |
20150017646 | SYSTEMS AND METHODS FOR MULTIPLEX ANALYSIS OF PCR IN REAL TIME - The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process. | 2015-01-15 |
20150017647 | TISSUE PROCESSING APPARATUS - An apparatus for processing a biological sample is provided. The biological sample being arranged on a first planar surface of a carrier, the apparatus having a second planar surface arranged substantially parallel to said first planar surface and at a first distance from said first planar surface. The first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP). The apparatus having a supply for supplying an amount of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other. | 2015-01-15 |
20150017648 | DEVICES, SYSTEMS AND METHODS FOR THERMAL CONTROL OF DROPLET DETECTION - A droplet detection system comprises a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel can meet at an intersection. The sample reservoir can include a sample or partition thereof. During use, an emulsion comprising one or more droplets can be generated at the intersection. The emulsion flows from the intersection along a detection channel to a collection reservoir. A detection assembly that is coupled to at least a portion of the detection channel is configured to detect a signal from the one or more droplets. An energy providing member can be in thermal communication with at least one of the carrier fluid reservoir, the sample reservoir, the intersection, the detection channel and the detection assembly. The energy providing member is configured to transfer energy to the emulsion. | 2015-01-15 |
20150017649 | COMPOSITIONS AND METHODS FOR DETECTING NOONAN SYNDROME - Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in a RAS-specific guanine nucleotide exchange factor gene SOS1 or its expression product. The diagnostic and therapeutic applications are also based on certain mutations in a serine/threonine protein kinase gene RAFl or its expression product thereof. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to RAF1 or SOS1, and variants thereof, as well as host cells expressing such variants. | 2015-01-15 |
20150017650 | REAGENTS AND METHODS FOR AUTOLIGATION CHAIN REACTION - The invention relates to the amplification of specific target nucleic acids. The invention provides methods, reagents, and kits for carrying out such amplification via the autoligation chain reaction (ACR). | 2015-01-15 |
20150017651 | PERSONALIZED MEDICINE FOR THE PREDICTION OF THERAPY TARGETING THE HEDGEHOG PATHWAY - Methods and composition for tumor therapy, especially esophageal adenocarcinoma (EAC), are described. For example, in certain aspects methods for determining Hedgehog and mTOR signaling pathway status to select patients for administering a combination therapy of Hedgehog and mTOR signaling inhibitors are described. Furthermore, the invention provides compositions that involve testing kits for determining signaling status. | 2015-01-15 |
20150017652 | Compositions and Method for Measuring and Calibrating Amplification Bias in Multiplexed PCR Reactions - Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification. | 2015-01-15 |
20150017653 | Absolute PCR Quantification - The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (PCR) on said volumes, detecting PCR amplification products, analyzing said detected PCR amplification products, performing absolute quantification of the PCR target and presenting said quantification results. | 2015-01-15 |
20150017654 | Absolute PCR Quantification - The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (PCR) on said volumes, detecting PCR amplification products, analyzing said detected PCR amplification products, performing absolute quantification of the PCR target and presenting said quantification results. | 2015-01-15 |
20150017655 | LABEL-FREE SEQUENCING METHOD FOR SINGLE NUCLEIC ACID MOLECULE - A label-free sequencing method for a single molecular nucleic acid is provided. The primer is paired with the nucleic acid template to be assembled to a polymerase. When the nucleotides are added, the electrical conductance signal is measured by the polymerase being connected to the protein transistor to determine the sequences of the nucleic acid template. The trajectory of the measured electrical conductance signal contains plateaux with obvious spikes, which is used to identify four types of the nucleotides and their bases. Furthermore, the sequencing method is suitable for sequencing of complex nucleic acids. | 2015-01-15 |
20150017656 | Rapid Lateral Flow Assay Method for Detecting Low Quantity Liquid or Dry Samples - This invention describes a design of a lateral flow assay device that detects dried chemicals or trice volume aqueous sample solutions, applicable for detecting body fluids and dried or liquid chemicals. The dried or aqueous samples on the sample loading area will contact with a secondary aqueous solution in described manner and flow to the reaction area. This invention enables a complete lateral flow assay while the sample volume itself is too small to accomplish a complete lateral flow test. | 2015-01-15 |
20150017657 | ASSAY AND METHOD FOR IDENTIFYING COMPOUNDS THAT INHIBIT EXCITOTOXIC SIGNALS - The invention relates to an assay for identifying compounds for the treatment of various diseases including those associated with excitotoxicity. The invention also relates to cell lines and constructs for use in an assay of the invention. The invention also relates to methods for determining whether a compound reduces excitotoxic signalling in a cell and whether a compound that inhibits binding of Tau to Fyn is likely to selectively reduce excitotoxic cell signalling. The invention also relates to a Tau protein adapted to form a detectable signal when the Tau protein is bound to a Fyn protein. The invention also relates to a Fyn protein adapted to form a detectable signal when the Fyn protein is bound to a Tau protein. | 2015-01-15 |
20150017658 | STAIN, PROCESS FOR STAINING AND ACQUIRING NORMALIZED SIGNAL - A kit includes a reference probe to react with a first sample; and an analyte probe to combine with a second sample, a marker and a spectral probe to react to form the analyte probe to combine with the second sample, the marker to react with the reference probe to form the analyte probe to combine with the second sample, or a combination thereof. A process for staining includes forming a reference composition by reacting a reference probe and a first sample to form a reference; and forming an analyte composition by combining an analyte probe and a second sample to form an analyte. | 2015-01-15 |
20150017659 | PROFILING OF SIGNAL PATHWAY PROTEINS TO DETERMINE THERAPEUTIC EFFICACY - The present invention provides methods for detecting, measuring and quantitating the activation states of components of the PI3K signaling pathway in cells such as tumor cells. In particular embodiments, the present invention enable the determination of tumor adaptation to anticancer therapy. Accordingly, the present invention provides methods for improved cancer therapy selection/adjustment and disease monitoring. | 2015-01-15 |
20150017660 | METHOD OF EXOSOME ANALYSIS, REAGENT FOR EXOSOME ANALYSIS, AND ANALYZER FOR EXOSOME - The present invention is to provide a method of exosome analysis that can analyze exosome in a sample in a simple manner. | 2015-01-15 |
20150017661 | METHODS FOR DETECTING 5T4-POSITIVE CIRCULATING TUMOR CELLS AND METHODS OF DIAGNOSIS OF 5T4-POSITIVE CANCER IN A MAMMALIAN SUBJECT - Methods are provided for detecting 5T4-positive circulating tumor cells in a mammalian subject. Methods of diagnosing 5T4-positive cancer in a mammalian subject are provided. The methods of detection or diagnosis indicate the presence of 5T4-positive metastatic cancer or early stage 5T4-positive cancer. | 2015-01-15 |
20150017662 | METHOD FOR DETECTING AND QUANTIFYING A TARGET PROTEIN OR A TARGET CELL USING AN APTAMER CHIP - The present invention is a method for detecting or quantifying a target cell or a target protein using an aptamer chip, and particularly, in order to detect a target cell, a method for detecting and/or quantifying a target cell by reacting a cell staining solution (e.g. 4′,6-diamidino-2-phenylindole (DAPI)) with an aptamer chip which the target cell is bound, in order to detect a target protein, a method for detecting and/or quantifying a target protein by reacting Coomassie Brilliant Blue solution with an aptamer chip which the target protein is bound, and a method for reusing the aptamer chip, wherein the aptamer chip comprises a board to which the aptamer specifically combining with the target cell or the target protein is bonded by a disulfide bond. | 2015-01-15 |
20150017663 | IDENTIFICATION OF ANTITUMOR COMPOUNDS USING PLACENTA - Provided herein are methods of evaluating potential antitumor compounds, and thereby identifying antitumor compounds, using placenta or a portion thereof and tumor cells, and compositions for accomplishing the same. In one embodiment, provided herein is a method of determining whether a potential antitumor compound is effective against a plurality of tumor cells, comprising introducing a plurality of tumor cells to, e.g., into or onto, a mammalian placenta or portion thereof; contacting said plurality of tumor cells for a period of time with said antitumor compound; and determining whether said antitumor compound is effective against said tumor cells, wherein said antitumor compound is effective against said tumor cells if said antitumor compound over said period of time reduces the number of said tumor cells or reduces the growth rate of said tumor cells. | 2015-01-15 |
20150017664 | GUT FLORA-DERIVED EXTRACELLULAR VESICLES, AND METHOD FOR SEARCHING FOR A DISEASE MODEL, VACCINE, AND CANDIDATE DRUG AND FOR DIAGNOSIS USING THE SAME - The present application relates to a composition including gut flora-derived extracellular vesicles, and to an animal disease model using same. In addition, the present application relates to a method for using the gut flora-derived extracellular vesicles to efficiently search for a candidate drug which may prevent or treat diseases that occur due to gut flora-derived extracellular vesicles, and to a vaccine which can efficiently prevent or treat infections caused by gut flora or diseases that occur due to gut flora-derived extracellular vesicles. Further, the development of diagnostic technology to discover, using the gut flora-derived extracellular vesicles of the present application, the etiology of diseases that occur due to gut flora-derived extracellular vesicles, can be achieved. | 2015-01-15 |
20150017665 | Peptides and Methods for Detecting MPO-ANCA - The present invention relates to disorder-specific epitopes recognized by myeloperoxidase-anti-neutrophil cytoplasmic autoantibodies and the use of polypeptides comprising the epitopes in methods of diagnosing and monitoring autoimmune disorders associated with the autoantibodies. | 2015-01-15 |
20150017666 | DIAGNOSTIC PEPTIDES FOR LYME DISEASE - The present invention relates, e.g., to a composition comprising peptides represented by SEQ ID NO:1-SEQ ID NO:28 and/or SEQ ID NO:41-SEQ ID NO:47, or active variants thereof, wherein the peptides or active variants can bind specifically to an antibody induced by a causative agent of Lyme disease (a pathogenic | 2015-01-15 |
20150017667 | MEANS AND METHODS FOR ASSESSING LIVER DISORDERS - The present invention pertains to the field of diagnostics for liver disorders and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing liver disorders. It also relates to a method for determining whether a compound is capable of inducing such liver disorders in a subject and to a method of identifying a drug for treating liver disorders. Furthermore, the present invention relates to a device and a kit for diagnosing liver disorders. | 2015-01-15 |
20150017668 | METHODS AND COMPOSITIONS FOR DIAGNOSING AND TREATING MOOD DISORDERS - The present invention relates to methods of diagnosing, prognosing or treating diseases or disorders in which elevated or reduced levels of Aβ protein, including Aβ | 2015-01-15 |
20150017669 | PROCESS AND ITS APPLICATION FOR IMPROVING REPRODUCIBILITY IN MALDI-TOF GLYCAN PROFILING OF HUMAN SERUM: EXPERIMENTAL PROCEDURE AND APPLICATION TO THE SCREENING FOR OVARIAN TUMORS - Disclosed is that the most crucial point in the quantitative analysis of MALDI-TOF MS is to minimize quantitative variances (RSDs, relative standard deviations) of peak intensities of obtained spectrum data. The minimized RSD is translated into improved reproducibility of the data and finally leads to high accuracy of the results. Several technical procedures based on the concept of automatization to reduce the RSDs (to less than 10%) in the MALDI-TOF MS of serum glycans were proposed for the preparation steps of extracting the targeted glycans from human blood and sample loading processes onto the plates. The presented techniques described in the examples proved the contributions of the present invention to enhanced reproducibility of the MALDI-TOF MS and consequently to improved screening accuracy for the differentiation of the benign ovarian tumor patients from the borderline ovarian tumor patients in comparison with accuracy of differentiation based on other analysis methods. | 2015-01-15 |
20150017670 | Methods For Determining The Degradation Of A Biomass Material - The present invention relates to methods for analyzing degradation of a biomass material at a high dry weight percentage. | 2015-01-15 |
20150017671 | METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY - This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor. | 2015-01-15 |
20150017672 | ASSAYS FOR DETECTION OF GLYCOSAMINOGLYCANS - Disclosed herein are novel methods, assays and kits useful for the diagnosis and monitoring of subjects with mucopolysaccharidoses (MPS). The methods, assays and kits are particularly useful for detecting the presence of one or more glycosaminoglycans which correlate to MPS and its severity in a variety of biological samples. | 2015-01-15 |
20150017673 | COMPOSITIONS AND METHODS FOR DRUG-SENSITIZATION OR INHIBITION OF A CANCER CELL - The disclosure provides rifamycin and rifamycin derivative compositions, including rifabutin and rifabutin derivative compositions able to cause drug-sensitization in a cancer cell or inhibition of a cancer cell. The disclosure also provides methods of administering such compositions to cancer cells to sensitize them to drugs, such as chemotherapeutics, or directly inhibit them. The disclosure also provides methods of administering such compositions to increase reactive oxygen species (ROS), particularly superoxides, in cancer cells. The disclosure further provides methods of determining whether a cancer will respond to chemotherapeutics and whether to administer rifamycin or a rifamycin derivative based on ROS levels in cancer cells of a patient. | 2015-01-15 |
20150017674 | METHOD FOR DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO VASCULAR BED CELLS - This application relates to a method for differentiating pluripotent stem cells (PSCs) into vascular bed cells. Moreover this application relates to a method for differentiating human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into vascular bed cells based on linked steps of chemically defined medium inductions. | 2015-01-15 |
20150017675 | ASSAY FOR DETERMINING RELATIVE REDOX CHANGES IN LIVING CELLS AND ASSOCIATED DEVICES, SYSTEMS, AND METHODS - The present disclosure provides conjugates, systems, devices, and methods for detecting cellular redox state. In one aspect, for example, a conjugate for detecting cellular redox state can include a first segment including a cell penetrating peptide conjugated to a first detection molecule, and a second segment including a cargo peptide conjugated to a second detection molecule, wherein the first segment and the second segment are coupled together by a redox-sensitive linkage, and wherein the first detection molecule and the second detection molecule have properties that allow linked proximity detection. In one specific example, the first detection molecule and the second detection molecule include fluorophore/quencher pair. | 2015-01-15 |
20150017676 | CAPSULES CONTAINING MAMMALIAN CELLS - A capsule containing at least one mammalian cell, includes a liquid core, and at least one external envelope totally encapsulating the liquid core at its periphery, the external envelope including at least one gelled polyelectrolyte and/or a stiffened biopolymer and being able to retain the liquid core when the capsule is immersed in a gas. The present invention further relates to the method for preparing such a capsule, to a method for screening cosmetic active ingredients as well as a culture method using such capsules. | 2015-01-15 |
20150017677 | CANCER STEM CELLS - Cancer stem cell populations characterized by expression of CD44 | 2015-01-15 |
20150017678 | Systems, Devices, and Methods for Separating, Concentrating, and/or Differentiating Between Cells from a Cell Sample - Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto. | 2015-01-15 |
20150017679 | THIN SECTION FABRICATION APPARATUS AND METHOD OF FABRICATING THIN SECTION - A thin section fabrication apparatus includes an epi-imaging data acquisition unit that performs imaging by radiating epi-illumination and acquires imaging data, a diffusion imaging data acquisition unit that performs imaging by radiating diffusion illumination and acquires imaging data, an exposed shape extraction unit that extracts an exposed shape of an exposure portion of the biological sample which is exposed to a surface of the embedding block, based on the imaging data acquired by the epi-imaging data acquisition unit, an embedded shape extraction unit that extracts an embedded shape of an embedding portion of the biological sample which is embedded in the embedding block, based on the imaging data acquired by the diffusion imaging data acquisition unit, and a control unit that determines ending of the preliminary cutting by comparing the exposed shape extracted by the exposed shape extraction unit and the embedded shape extracted by the embedded shape extraction unit. | 2015-01-15 |
20150017680 | TEST APPARATUS FOR TESTING THE MICROBIAL ACTIVITY ON SURFACES - The present invention relates to a device comprising a sample receiving appliance, a receiving appliance for test organisms, preferably for a suspension comprising test organisms, in particular for a bacterial suspension, and a jet pump appliance, wherein the jet pump appliance is, or can be brought, into active connection with the receiving appliance, and wherein the jet pump appliance is designed and installed in order, by means of a propellant medium having a higher pressure than atmospheric pressure at the location of installation of the device to spray test organisms in the form of an aerosol in the direction of the sample receiving installation, wherein the device has an installation for controlling a reproducible pressure of the propellant medium during the spraying of the test organisms and also the use of a device according to the invention. | 2015-01-15 |
20150017681 | DEVICE AND METHOD FOR BIOLOGICAL SAMPLE COLLECTION AND INSPECTION - A device is described including a lid, a collection portion support, and a collection portion with a surface for receiving a biological sample, the lid includes a window and a window holder, the surface defines a first plane, the collection portion includes a first, a second, and a third position markers, each position marker indicates a center point marker, the center points define a second plane that has a line of intersection with the first plane, the device has an interior volume defined partially by the collection portion support, the collection portion, the window, and the window holder, the window allows visual inspection of the position markers, the collection portion support has a fluid inlet and a fluid outlet with a fluid path from the fluid inlet to the fluid outlet via the interior volume. | 2015-01-15 |
20150017682 | ANASTOMOTIC LEAKAGE DETECTION THROUGH DUAL ACTION CATHETER - Technologies are generally described for detecting the presence of anastomotic leakage in the abdominal cavity through a dual action catheter placed in the vicinity of a surgery site. A portion of the dual action catheter may be affixed around the surgery site, for example, coiled around the surgery site on the intestine. Through openings on the catheter near the surgery site, a neutral fluid such as saline solution may be injected and extracted through the same openings resulting in retrieval of fluids in the same are. By testing the extracted fluids, chemical and biochemical activity within the abdominal cavity may be determined in order to gauge the start of any anastomotic leakage. | 2015-01-15 |
20150017683 | Stacked Membrane Bioreactor - Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described. | 2015-01-15 |
20150017684 | STREPTOCOCCUS PYOGENES ANTIGENS AND CORRESPONDING DNA FRAGMENTS - The present invention relates to antigens more particularly antigens of | 2015-01-15 |
20150017685 | EFFICIENT PRODUCTION OF PEPTIDES - The present invention relates to processes for the production of peptides, and the peptides produced accordingly. Peptides produced according to the invention may be produced more efficiently than peptides produced according to prior art processes. The production process of the invention may lead to advantages in yield, purity, and/or price. Methods of marketing peptides are also disclosed. | 2015-01-15 |
20150017686 | METHODS FOR REDUCING MANNOSYLTRANSFERASE ACTIVITY IN LOWER EUKARYOTES - Disclosed are methods for reducing detectable mannosylation of N-linked and O-linked oligosaccharides in lower eukaryote host cells. In particular, recombinant lower eukaryote host cells are provided in which expression of the GDP-mannose transporter encoded by the Vanadate Resistant Glycosylation 4 (VRG4) gene has been disrupted. In general, the VRG4 gene is essential for cell viability; however, the present invention provides host cells that are viable when expression of the VRG4 gene therein has been disrupted. The host cells are capable of producing proteins or glycoproteins that have reduced or no detectable α-linked mannose, β-linked mannose or phosphomannose containing N- and/or O-glycans. | 2015-01-15 |
20150017687 | METHOD FOR OBTAINING A GLYCOPROTEIN COMPOSITION - Provided is a method for producing glycoprotein with a specific glycoform compostion. The desired glycoform profile is brought about Iby altering the culture conditions on the basis of IVCC rather than the age of the culture. Further, the method renders a high product yield. | 2015-01-15 |
20150017688 | COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) - The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes. | 2015-01-15 |
20150017689 | -GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 2015-01-15 |
20150017690 | BETA-GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 2015-01-15 |
20150017691 | -GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 2015-01-15 |
20150017692 | BETA-GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 2015-01-15 |
20150017693 | Auto-Indicible Expression System, and the Use Thereof for Producing Useful Metabolites Using a Bacterium of the Family Enterobacteriaceae - The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus | 2015-01-15 |
20150017694 | Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same - Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins. | 2015-01-15 |
20150017695 | KETOREDUCTASE POLYPEPTIDES FOR THE REDUCTION OF ACETOPHENONES - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. | 2015-01-15 |
20150017696 | RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A CROTONOL INTERMEDIATE - The present disclosure relates to recombinant host cells comprising one or more recombinant polynucleotides encoding enzymes in select pathways that provide the ability to use the cells to produce 1,3-butadiene. The present disclosure also provides methods of manufacturing the recombinant host cells, and methods for the use of the cells to produce 1,3-butadiene, either through formation of the intermediate compound crotonol followed by chemo-catalytic dehydration to 1,3-butadiene, or through the use of a recombinant cell comprising a fully enzymatic pathway capable of converting crotonyl-CoA or crotonyl-ACP to crotonol and then crotonol to 1,3-butadiene. | 2015-01-15 |
20150017697 | Anaerobic process for producing biogas with a high methane content by means of the biodigestion of organic waste - This invention is about a process of increased Biogas ( | 2015-01-15 |
20150017698 | RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A 5-HYDROXYPENT-3-ENOATE INTERMEDIATE - The present disclosure relates to recombinant host cells comprising one or more recombinant polynucleotides encoding enzymes in select pathways that provide the ability to use the cells to produce 1,3-butadiene. The present disclosure also provides methods of manufacturing the recombinant host cells, and methods for the use of the cells to produce 1,3-butadiene. The methods utilize recombinant host cells that comprise an engineered pathway of enzymes that provides for the conversion of naturally occurring intermediate crotonyl-CoA (or -ACP) to 1,3-butadiene through enzyme catalyzed steps involving the reduction of glutaconyl-CoA (or -ACP) to form the intermediate 5-hydroxypent-3-enoate. The disclosure provides alternative engineered pathway involving either decarboxylation of 5-hydroxypent-3-enoate directly to 1,3-butadiene, or phosphorylation of 5-hydroxypent-3-enoate followed by a phosphate elimination step catalyzed by a decarboxylase to produce 1,3-butadiene. | 2015-01-15 |
20150017699 | Internally functionalized graphene substrates - The present invention is generally directed to internally functionalized graphene substrates, methods of making such substrates and methods of using such substrates. In one aspect, the present invention is a graphene substrate. The substrate comprises edge and non-edge regions. Organic or inorganic molecules are bound to the non-edge regions of the substrate, and the organic or inorganic molecules are present on the substrate edges at a population greater than about one molecule per 10,000 nm | 2015-01-15 |
20150017700 | COMPOSITIONS AND METHODS COMPRISING A LIPOLYTIC ENZYME VARIANT - The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having two, three, or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention. | 2015-01-15 |
20150017701 | HYBRID ALPHA-AMYLASES - Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like α-amylase, e.g., a | 2015-01-15 |
20150017702 | CRYSTALLIZATION SUBSTRATE, CRYSTALLIZATION CONTAINER, CRYSTALLIZATION DEVICE, AND CRYSTAL PRODUCING METHOD - A crystallization substrate of the present invention includes a noble metal vapor-deposited film having an absorbance in a 500 to 1,000 nm wavelength range and formed in all or part of one surface of the substrate. The noble metal vapor-deposited film has an average thickness of 0.1 to 60 nm. The noble metal vapor-deposited film is a continuous film with a pit formed by vapor deposition in part of the film and surrounded by the continuous film. | 2015-01-15 |
20150017703 | METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES - Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome. | 2015-01-15 |
20150017704 | Cyanobacterium sp. Host Cell and Vector for Production of Chemical Compounds in Cyanobacterial Cultures - A cyanobacterial host cell, | 2015-01-15 |
20150017705 | Method and a system for mass-cultivating microalgae with enhanced photosynthetic efficiency - A method for cultivating photosynthetic microorganisms with optimal photosynthetic efficiency, comprising the steps of exposing the microorganisms grown in a culture tank of culture broth to light and carbon source; circulating the culture broth vertically from the bottom of the tank up to the surface of the tank to allow momentary exposure of the microorganisms to the light and carbon source, using one or more circulating means located within the culture tank which are regulated according to a selected cultivation mode; and maintaining the cultured microorganisms at a substantially constant areal density range of 300 to 12,000 g/m | 2015-01-15 |
20150017706 | CAPTURED CARBON DIOXIDE FOR ALGACULTURE - Enhancing growth of algae in an algaculture facility includes contacting a growth medium with a gas including carbon dioxide, transferring some of the carbon dioxide to the growth medium to yield an enriched growth medium, and providing the enriched growth medium to the algaculture facility. The concentration of dissolved carbon dioxide in the enriched growth medium exceeds the concentration of dissolved carbon dioxide in the growth medium, where dissolved carbon dioxide includes ions formed by the reaction of carbon dioxide with a species in solution. The growth medium may be obtained from the algaculture facility, and may be filtered or otherwise processed before or after contacting the growth medium with the gas. Providing the enriched growth medium to the algaculture facility increases the concentration of dissolved carbon dioxide in the bulk growth medium of the algaculture facility. | 2015-01-15 |
20150017707 | STORAGE-STABLE LIQUID DISHWASHING DETERGENT CONTAINING PROTEASE AND AMYLASE - In a liquid dishwashing detergent comprising a protease and amylase, storage stability is to be improved. This is achieved through the use of a protease comprising an amino acid sequence having at least 70% identity over its total length with the amino acid sequence specified in SEQ ID NO. 1 and having, in the listing according to SEQ ID NO. 1, the L211D amino acid substitution in combination with at least two further amino acid substitutions selected from the group consisting of S3T, V4I, V193M and V199I. | 2015-01-15 |
20150017708 | COMPOSITION FOR ENZYMATIC OIL DEGUMMING - The invention relates to a composition comprising at least one phospholipid-cleaving enzyme. The invention further relates to a method for degumming raw oils using the composition according to the invention and to the use of the composition according to the invention to degum raw oils. | 2015-01-15 |
20150017709 | METHOD FOR DETECTING THE PRESENCE OF A TARGET NUCLEIC ACID SEQUENCE IN A SAMPLE - A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber. | 2015-01-15 |
20150017710 | APPARATUS FOR TESTING, ASSESSMENT, AND MAINTENANCE OF HARVESTED HEARTS FOR TRANSPLANTING - An apparatus, a system, and methods for receiving, perfusing and maintaining and assessing excised donor heart physiological functionality, The system generally comprises an apparatus for receiving and holding an excised heart that is interconnected with: (i) a perfusate-processing system, (ii) a bi | 2015-01-15 |
20150017711 | CELL CULTURE SYSTEMS - An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels. | 2015-01-15 |
20150017712 | MASS SPECTROMETRY ANALYSIS OF MICROORGANISMS IN SAMPLES - The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples. | 2015-01-15 |
20150017713 | Micro Fluid Dosing Unit and Testing Device for Biomaterial - A micro fluid dosing unit includes a test container containing biomaterial needing oxygen. The test container has at least one first container which contains water and at least one second container which contains a pharmacological substance, wherein the first and the second containers have a container outlet leading to the test container and are mounted on a common valve carrier plate containing control channels, which includes control channel ports for control valves to be coupled thereto. In the valve carrier plate a separate valve control chamber is formed for each container, which is sealed by a membrane, wherein associated container outlets are opened and closed by the membrane movement. | 2015-01-15 |
20150017714 | CENTRIFUGATION CHAMBER WITH GAS PERMEABLE MEMBRANE LAYERS FOR CELL CULTIVATION - The invention is directed to a centrifuge chamber comprising a cylinder having a base plate and a cover plate, a rotational axis with at least one port for input and/or output of liquids, at least one port for input of gases and at least one layer for cell culturing, wherein the layer for cell culturing comprises a gas-permeable membrane on which cells can be cultured and wherein the at least one port for input and/or output of gases is connected to the gas-permeable membrane. | 2015-01-15 |
20150017715 | CELL COLLECTION DEVICE AND METHOD - A compact and efficient cell collection device and method based on biologically-derived cell rolling and a planar collection module that provides fluid recirculation and modification of shear forces to discriminate between target and non-target cells. | 2015-01-15 |
20150017716 | ONE-WAY SEPARATOR FOR RETAINING AND RECIRCULATING CELLS - The invention relates to a separator for retaining and recirculating cells in a continuous-flow or batch-flow type plastic bag or bottle, which can preferably be operated outside of a bioreactor. Additionally, the invention relates to a method for retaining and recirculating cells within or outside a bioreactor. The invention further relates to a method for producing the separator according to the invention. | 2015-01-15 |
20150017717 | CULTURE MEDIUM FOR EUKARYOTIC CELLS - The invention pertains to the use of C | 2015-01-15 |
20150017718 | METHOD FOR INDUCING CARDIAC DIFFERENTIATION OF PLURIPOTENT STEM CELL - The present invention relates to a method for inducing cardiac differentiation of a pluripotent stem cell, which comprises the steps of
| 2015-01-15 |
20150017719 | Securing Apparatus and Method - A securing apparatus includes a housing, a cam member, and a movable device that can be moved in a first direction to engage a first surface of the cam member to cause the cam member to move from a first position to a second position. When the cam member is in the first position, first and second movable members can be moved relative to the housing. When the cam member is in the second position, second and third surfaces of the cam member press respectively against the first and second movable members to trap the first and second movable members respectively between the second and third surfaces of the cam member and the housing such that the first and second movable members are fixed relative to the housing. | 2015-01-15 |
20150017720 | THERAPEUTIC USES OF CURCUMIN ANALOGS FOR TREATMENT OF PROSTATE CANCER - This invention relates generally to methods of treating aggressive cancers, such as hormone-refractory metastatic prostate cancer, by exposing the aggressive cancer cells to curcumin analogs having the claimed structural scaffolds and side groups. The anticancer effects of curcumin are associated with its influence on numerous growth factors within the cells. However, its clinical development has been limited by its suboptimal pharmacokinetics and poor bioavailability caused by poor solubility in water and rapid in vivo metabolism. There is the need to develop new and improved curcumin analogs with better potency, water solubility, and in vivo metabolic stability, as well as retained safety profiles. Curcumin analogs having one of the claimed four novel scaffolds with basic heteroaromatic side groups show the ability to decrease aggressive cancer cell viability and to inhibit aggressive cancer cell growth. | 2015-01-15 |
20150017721 | METHOD FOR INDUCING PROLIFERATION OF NATURAL KILLER CELLS BY MOBILE NANOMATRICES - The present invention provides an in-vitro method for inducing proliferation of NK cells, the method comprising contacting a population of NK cells with a nanomatrix, the nanomatrix comprising a) a matrix of mobile polymer chains; and b) attached to said matrix of mobile polymer chains one or more stimulatory agents which provide activation signals to the NK cells; thereby activating and inducing the NK cells to proliferate; wherein the nanomatrix is 1 to 500 nm in size. At least one first and one second stimulatory agents are attached to the same or to separate flexible matrices. If the stimulatory agents are attached to separate beads, fine-tuning of nanomatrices for the proliferation of the NK cells is possible. Closed cell culture systems also benefit from this method. | 2015-01-15 |
20150017722 | DOUBLE-STRANDED AND SINGLE-STRANDED RNA MOLECULES WITH 5' TRIPHOSPHATES AND THEIR USE FOR INDUCING INTERFERON - Double-stranded and single-stranded RNA molecules, and their use in methods for inducing interferon are provided. The interferon induction provides anti-viral and other medically useful effects, such as anti-cancer effects. Also provided are methods for reducing or inhibiting interferon induction exhibited by such molecules, particularly siRNA and shRNA molecules produced in vitro. | 2015-01-15 |
20150017723 | PROCESS OF EXPANDING T CELLS - The present disclosure relates to a novel process for expanding T cells, such as autologous T cells, cell populations therefrom, pharmaceutical compositions comprising the said cell populations and use of the cells and compositions for treatment, particular the treatment or prophylaxis of virus infection and/or cancer, for example in immune compromised or immune competent human patients. | 2015-01-15 |
20150017724 | METHOD FOR GUIDING THE DERIVATION OF ENDOTHELIAL CELLS FROM HUMAN PLURIPOTENT STEM CELLS EMPLOYING TWO-DIMENSIONAL, FEEDER-FREE DIFFERENTIATION - This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, by plating a single-cell suspension of PSCs onto a suitable surface such as type IV collagen and culturing the cells with VEGF after which ECs can be harvested. A preferred embodiment of the method first cultures the cells without VEGF and then sequentially cultures the cells with VEGF. Differentiation can be enhanced by adding an inhibitor of transforming growth factor β to the culturing with VEGF. | 2015-01-15 |
20150017725 | PRODUCTION METHOD OF POLYURETHANE POROUS MEMBRANE TO BE USED FOR AT LEAST ONE OF APPLICATIONS OF CELL CULTURE AND CANCER CELL GROWTH INHIBITION - A polyurethane porous membrane is produced by a simple method to be used for at least one of applications of cell culture and cancer cell growth inhibition. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition comprises: a first step of forming a layer of a polyurethane material which is uncured, on a substrate; and a second step of supplying water vapor to an exposed surface of the layer of the polyurethane material formed on the substrate, which is away from the substrate, so as to cure the polyurethane material and provide the layer of the polyurethane material with a porous structure having a plurality of irregularities on the exposed surface. | 2015-01-15 |
20150017726 | MEDIA FOR STEM CELL PROLIFERATION AND INDUCTION - The present application discloses a cell culture media for growth, maintenance and induction of reversion to a less mature state of a cell comprising a MUC1* activating ligand. | 2015-01-15 |
20150017727 | METHODS OF INDUCING REGULATED PANCREATIC HORMONE PRODUCTION IN NON-PANCREATIC ISLET TISSUES - Disclosed are methods and pharmaceutical compositions for inducing pancreatic hormone production. | 2015-01-15 |
20150017728 | MONOMER ARCHITECTURE OF TAL NUCLEASE OR ZINC FINGER NUCLEASE FOR DNA MODIFICATION - The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding or a zinc finger domain and an I-TevI homing endonuclease cleavage domain as well as nucleic acid sequence encoding the same. The use of the I-TevI domain allows for monomer endonuclease sequences to achieve cleavage of cellular chromatin and represents an advantage over prior endonucleases which require self-dimerization, and two nucleases with appropriate spacers. | 2015-01-15 |
20150017729 | METHOD FOR EVALUATION TESTING OF MATERIAL FOR INTERNAL COMBUSTION ENGINE - An oxide film is formed on the surface of a sample made from a metal material by holding the above-described sample at a temperature of 800° C. or higher and 1,100° C. or lower in an oxygen-containing atmosphere, and the sample provided with the oxide film is immersed in a corrosive solution containing an acid and NaCl for a predetermined time. After immersion, the corrosion state (degree of denseness of oxide film, cracking state, and the like) of the sample is evaluated. The corrosion resistance of the sample can be evaluated appropriately and conveniently in a short period of time by causing accelerated corrosion in an environment simulating the actual environment of an internal combustion engine. | 2015-01-15 |
20150017730 | UREA SOLUTION QUALITY DETERMINING SYSTEM - A urea solution quality determining system may include a urea tank in which a urea solution is filled through a charging hole, a test chamber positioned below the charging hole for holding a portion of the urea solution, a main line connected to the urea tank at a lower part of the urea tank below the test chamber, a test line connected to the test chamber, a pumping line to which the main line and the test line are joined, a pump in the pumping line to pump the urea solution from the test chamber or from the urea tank, a control valve for selectively connecting the main line or the test line to the pumping line, and an injector at an end of the pumping line to inject the urea solution into an exhaust line through which an exhaust gas flows. A method is also described. | 2015-01-15 |
20150017731 | TEST APPARATUS AND METHOD OF ACCELERATED PHOTO-DEGRADATION USING PLASMA LIGHT SOURCE - Disclosed is a test apparatus of accelerated photo-degradation measuring a degradation caused by photo-degradation in a material or a product by light containing UV light and visible light that simulate the solar light-inducing degradation. The apparatus includes a light irradiating unit configured to irradiate light using at least one electrodeless plasma lamp; a light condensing unit configured to collect light emitted from the light irradiating unit and irradiate the light uniformly into the surface of the specimen for the material or the product; and a specimen holder configured to fix the specimen, and the specimen holder being fixed at a predetermined position or rotated. Further, the at least one electrodeless plasma lamp generates light having a light power spectrum similar to a solar light in a region of UV light and short-wavelength visible light corresponding to a range of about 300-500 nm. | 2015-01-15 |
20150017732 | COLORIMETRIC METHOD TO DETECT ILLICIT DRUGS - A colorimetric assay to detect certain compounds and a kit therefore are provided. | 2015-01-15 |
20150017733 | Method and Device for Monitoring Medication Usage - The present invention provides methods for detecting and quantifying metabolites in a biological sample by measuring the concentration of a test metabolite in the sample and comparing that concentration against the concentration of the reference metabolite; enabling accurate metabolite concentration measurements to determine aberrant drug usage patterns. Also disclosed is an analytical testing device and related computer-assisted products for detecting and quantifying metabolites in a biological sample efficiently and accurately. | 2015-01-15 |
20150017734 | QUALITY CONTROL MARKER AND ITS USE IN HERBS AUTHENTICATION - The present invention relates to a quality control marker and method of using such marker in qualitative and quantitative authentication of | 2015-01-15 |
20150017735 | Analyte sensor and analyte sensing method - A biosensor includes a detection element having an analyte detecting portion which is monotonically increased in mass in response to detection of an analyte; a reference element having a reference measuring portion which exhibits no reactivity to the analyte; a mixer which mixes a detection signal responsive to mass variations in the analyte detecting portion from the detection element and a reference signal from the reference element; a measurement which calculates two candidate phase-change values of a positive value and a negative value, from a signal mixed by the mixer in accordance with a heterodyne system, and determines a phase-change value from the two candidate phase change value by judging whether the phase is positive or negative based on temporal changes in signal strength; and a detection amount calculation portion which calculates a detection amount of the analyte based on the phase change value determined by the measurement portion. | 2015-01-15 |
20150017736 | METHOD FOR THE NON-DESTRUCTIVE TESTING OF WORKPIECE SURFACES - Disclosed is a method for the non-destructive testing of workpiece surfaces of a workpiece by means of fluorescent penetrant testing or dye penetrant testing. The method comprises applying a penetrant to the region of the workpiece surface to be examined, thereby allowing the penetrant to penetrate into possible recesses in the workpiece surface, applying a developer to the region of the workpiece surface to be tested; bleaching the penetrant by a gaseous or liquid oxidant; and visually assessing the penetrant that has remained in the recesses present in the workpiece surface. | 2015-01-15 |
20150017737 | APPARATUS AND METHOD FOR SEPARATING PLASMA FROM BLOOD AND DELAYED WETTING - Devices and methods are disclosed herein for separating a supernate from a suspension. The apparatus consists of a sample zone, a controllable gate, and an analysis zone. The sample zone holds the suspension. The analysis zone passively transports a supernate formed from the suspension by capillary transport. A controllable gate prevents the suspension in the sample zone from flowing into the analysis zone. The controllable gate can be triggered after the supernate has separated from the suspension to allow the supernate to flow into the analysis zone. | 2015-01-15 |
20150017738 | MEANS AND METHODS OF MEASURING PARATHYROID HORMONE IN PATIENTS SUFFERING FROM OXIDATIVE STRESS - Method for obtaining an antibody or antibody fragment to a conformational epitope specific for oxidized, inactive human parathyroid hormone and fragments thereof; a method for removal of oxidized, inactive human parathyroid hormone from a sample of body fluid; methods of determining the concentration of active parathyroid hormone in a sample, and an in vitro method of diagnosis of renal failure or secondary hyperthyroidism in patients on dialysis. The antibody is obtained by immunizing an animal with an immunogen containing parathyroid hormone or fragment thereof oxidized at methionines at positions 8, 18 or both; and a recovering of antibodies; whereby the complementary determining region of the antibody or antibody fragment or single chain antibody specifically recognizes a conformational epitope (antigenic determinant) which is a tertiary structure and only present on oxidized parathyroid hormone and fragments thereof only but not regular bioactive human parathyroid hormone, while the binding surface to the complementary binding region does not include any one of the oxidized methionines of human parathyroid hormone. | 2015-01-15 |
20150017739 | CONFORMATIONAL-SWITCHING FLUORESCENT PROTEIN PROBE FOR DETECTION OF ALPHA SYNUCLEIN OLIGOMERS - A conformation-switching fluorescent protein probe for detection of alpha synuclein oligomers. The use of intrinsically disordered proteins as conformation-switching biosensors. The use of an alpha synuclein (αS) variant, PG65, together with conformation-sensitive fluorescence to create a molecular probe for rapid, specific and quantitative detection of αS oligomers. | 2015-01-15 |
20150017740 | MOLECULAR SENSOR BASED ON VIRTUAL BURIED NANOWIRE - The present invention provides a method and a system based on a multi-gate field effect transistor for sensing molecules in a gas or liquid sample. The said FET transistor comprises dual gate lateral electrodes (and optionally a back gate electrode) located on the two sides of an active region, and a sensing surface on top of the said active region. Applying voltages to the lateral gate electrodes, creates a conductive channel in the active region, wherein the width and the lateral position of the said channel can be controlled. Enhanced sensing sensitivity is achieved by measuring the channels conductivity at a plurality of positions in the lateral direction. The use of an array of the said FTE for electronic nose is also disclosed. | 2015-01-15 |