06th week of 2009 patent applcation highlights part 44 |
Patent application number | Title | Published |
20090035766 | Methods for Analyzing High Dimension Data for Classifying, Diagnosing, Prognosticating, and/or Predicting Diseases and Other Biological States - A method of diagnosing, predicting, or prognosticating about a disease that includes obtaining experimental data, wherein the experimental data is high dimensional data, filtering the data, reducing the dimensionality of the data through use of one or more methods, training a supervised pattern recognition method, ranking individual data points from the data, wherein the ranking is dependent on the outcome of the supervised pattern recognition method, choosing multiple data points from the data, wherein the choice is based on the relative ranking of the individual data points, and using the multiple data points to determine if an unknown set of experimental data indicates a diseased condition, a predilection for a diseased condition, or a prognosis about a diseased condition. | 2009-02-05 |
20090035767 | PRIMER FOR BACTERIUM GENOME AMPLIFICATION REACTION - A primer or a primer set can efficiently amplify the gene sequence of 16S rRNA of a target bacterium out of selected sixty two bacterium species. At least one of the primers having a base sequence selected from sequences No. 1 through No. 6 is employed as bacterium genome amplification reaction primer: | 2009-02-05 |
20090035768 | Method of Determining Predisposition to Scoliosis and Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 2009-02-05 |
20090035769 | Genetic Markers Associated with Scoliosis and Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 2009-02-05 |
20090035770 | Inline-injection microdevice and microfabricated integrated DNA analysis system using same - Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing. | 2009-02-05 |
20090035771 | Hepatocellular carcinoma-related genes and polypeptides, and method for detecting hepatocellular carcinomas - Genes up-regulated in hepatocellular carcinomas and polypeptides encoded by these genes are provided. Vectors, transformants and methods for producing the recombinant polypeptides are also provided. Probes and primers of these genes and antibodies against the polypeptides are also provided. The probes, primers and antibodies can be used as reagents for detecting hepatocellular carcinomas. Methods for detecting hepatocellular carcinomas using such detection reagents are further provided. Antisense nucleotide sequences of these genes are also provided and can be used to inhibit growth of hepatocellular carcinomas. | 2009-02-05 |
20090035772 | Genetic Markers Associated With Scoliosis And Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 2009-02-05 |
20090035773 | NUCLEIC ACID SEQUENCES FOR DETECTING GENETIC MARKERS FOR CANCER IN A BIOLOGICAL SAMPLE - Nucleic acid sequences for detecting the presence of nucleic acids, particularly mRNA, encoding human prostate-associated genetic markers encoding prostate-specific antigen (PSA), prostate specific membrane antigen (PSMA) or human kallikrein 2 (hK2) are disclosed. Preferred combinations of nucleic acid sequences amplifying and detecting the prostate-associated genetic markers RNA, used in methods that include amplification of the target sequences and detection of the amplified sequences are disclosed. Methods of detecting the presence of prostate-associated genetic marker nucleic acids, particularly mRNA, in a biological sample of non-prostate origin are disclosed. | 2009-02-05 |
20090035774 | METHOD FOR CHARACTERIZING PRIMARY TUMORS - The invention relates to a method for the detection and characterisation of primary tumours and separate areas of primary tumours, respectively. Clusters of tumour cells, extracted from sample material, are isolated and concentrated, followed by an analysis of genetic changes in these isolated cell clusters. | 2009-02-05 |
20090035775 | PCR-BASED DETECTION METHOD FOR CHLAMYDIA TREACHOMATIS - A process for designing of PCR-based detection method for | 2009-02-05 |
20090035776 | Method and Kit for Hla-B Genotyping Based on Real-Time Pcr - Method and kit based on real-time PCR with specific primers and probes that allow to achieve a high degree of subtyping of the complete HLA-B locus. The main advantages are the greater speed (65 minutes, including the interpretation); the ease of automation, since only eighteen tubes are necessary to obtain a good level of resolution (typing of 300 groups); reduction of the total cost per test thanks to the ease of automation and the simplicity; a surprisingly high degree of allele definition is achieved; and the risk of sample contamination is reduced because the amplified products always remain in the tubes and no post-PCR steps are necessary. | 2009-02-05 |
20090035777 | HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION - Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers and oligomeric and monomeric substrate constructs are also disclosed. | 2009-02-05 |
20090035778 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH MULTIPLE SCLEROSIS - The present invention provides, in certain aspects, a method of identifying a subject as having an increased risk of developing multiple sclerosis, comprising detecting in the subject the presence of a nucleotide variant in the interleukin 7 receptor alpha chain gene, whereby the presence of said variant identifies the subject as having an increased risk of developing multiple sclerosis. | 2009-02-05 |
20090035779 | SYSTEMS AND METHODS FOR DETERMINING CROSS-TALK COEFFICIENTS IN PCR AND OTHER DATA SETS - Systems and methods for determining cross-talk coefficients in curves, such as sigmoid-type or growth curves, and PCR curves and nucleic acid melting curves in particular, as well as for applying the cross-talk coefficients to produce cross-talk corrected data sets using a linear subtractive model. Cross-talk signal coefficients are determined using cross-talk data acquired across the entire signal acquisition range. Analyzing across all of the signal curve data provides for a more robust cross-talk correction across the entire data acquisition range. A linear subtractive model is used to correct data sets having cross-talk components. | 2009-02-05 |
20090035780 | DETECTION OF METHICILLIN-RESISTANT AND METHICILLIN-SENSITIVE STAPHYLOCOCCUS AUREUS IN BIOLOGICAL SAMPLES - Disclosed are methods of identifying a methicillin-resistant | 2009-02-05 |
20090035781 | METHODS AND COMPOSITIONS FOR IDENTIFYING A SUBJECT WITH AN INCREASED RISK OF GRAM NEGATIVE BACTERIAL INFECTION - The present invention provides method of identifying a subject as having an increased risk of developing a Gram negative bacterial infection and/or as having an increased risk of mortality, comprising genotyping the subject for the presence of particular alleles of the lipopolysaccharide binding protein gene, wherein the presence of said allele(s) identifies the subject as having an increased risk of developing a Gram negative bacterial infection and/or of having an increased risk of mortality. | 2009-02-05 |
20090035782 | BREEDING PLANTS - A process for breeding plants which comprises growing plants of a species in an array of containers charged with growing medium of uniform characteristics in an environment of controlled climatic conditions with controlled supply of nutrients and feed water and changing the positions of the containers within the environment as required to ensure at least substantially uniform exposure of all plants in the containers to conditions in the environment. A process for the breeding of open pollinating plants in a greenhouse environment is also provided. A process for breeding plants which comprises identifying trait leads. | 2009-02-05 |
20090035783 | FLUORESCENT PROTEINS AND USES THEREOF - A fluorescent sensor and methods for producing and using the fluorescent sensor. Such fluorescent sensors have broad applicability in characterizing cells and organisms, in detecting or measuring various cellular parameters, and in detecting or measuring protein-protein/peptide interactions. | 2009-02-05 |
20090035784 | NPC1L1 AND NPC1L1 INHIBITORS AND METHODS OF USE THEREOF - The present invention provides a novel gene, designated herein as “NPC1L1”, that is associated with lipid or glucose metabolism. The invention further provides the use of the NPC1L1 gene and its corresponding protein to diagnose a lipid condition in a cell or tissue and to screen for novel therapeutic compounds useful for treating lipid disorders and other NPC1L1-associated or mediated diseases or disorders. The invention further provides specific inhibitors of NPC1L1. | 2009-02-05 |
20090035785 | IMMUNOASSAY METHOD AND REAGENT THEREFOR - The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase. | 2009-02-05 |
20090035786 | MULTI-ANALYTE AFFINITY COLUMN - A multi-analyte column is disclosed. The column may contain at least one unit of resin having ochratoxin specific affinity and, for each unit of resin having ochratoxin specific affinity, the column further contains about 0.95 to 1.05 units of resin containing antibody having specificity for zearalenone, about 1.9 to 2.1 units of resin containing antibody having specificity for aflatoxin, about 2.35 to 2.65 units of resin containing antibody having specificity for fumonisin, about 2.8 to 3.2 units of resin containing antibody having specificity for T-2 (and/or HT-2) and about 4.7 to 5.3 units of resin containing antibody having specificity for deoxynivalenol. One unit of resin is the quantity of resin containing antibody that will bind 50 ng of aflatoxin, 500 ng of deoxynivalenol, 3300 ng of fumonisin, 50 ng of ochratoxin, 830 ng T-2 (and/or HT-2) or 1140 ng of zearalenone, respectively. | 2009-02-05 |
20090035787 | ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES WITHOUT IDURONIC ACID RESIDUES - Iduronic acid (IdoUA)-free heparan sulfate (HS)-like compounds are provided. Also provided are methods of synthesizing IdoUA-free HS-like compounds. The methods can include providing at least one O-sulfotransferase (OST) enzyme and a reaction mixture comprising 3′-phosphoadenosine 5′-phosphosulfate (PAPS); and incubating a polysaccharide substrate with the at least one OST and the reaction mixture, whereby the HS-like compounds are synthesized. Also disclosed are methods of synthesizing a library of HS-like compounds and methods of determining the mechanism of activity of HS-like compounds. | 2009-02-05 |
20090035788 | Genetically encoded bioindicators of calcium-ions - The present invention relates to novel types of cellular calcium probes that are based on Troponin C and two chromophors suitable for FRET (fluorescence resonance energy transfer). The Troponin C-based calcium sensors of the invention function in diverse subcellular environments, for example even when tethered to a cellular membrane. The invention further provides nucleic acid constructs encoding the calcium probes of the invention, expression constructs, host cells and transgenic animals. Furthermore, methods for the detection of changes of local calcium concentrations and for detecting the binding of a small molecule to fragments of Troponin C are provided. | 2009-02-05 |
20090035789 | MONITORING ENZYME MIXTURES - This invention provides a method for simultaneously detecting the presence of at least two enzymes in a sample, said method comprising the steps of; i) providing a first substrate for a first enzyme, said first substrate being labeled with a first fluorophore, ii) providing a second substrate for a second enzyme, said second substrate being labeled with a second fluorophore, iii) exposing the labeled substrates to the sample to allow the first and second enzymes present in the sample to interact with respective first and second fluorophore-labeled substrates to form respective first and second fluorophore-labeled substrate fragments; and, detecting the presence of said fluorophore-labeled substrate fragments. | 2009-02-05 |
20090035790 | HIGH SENSITIVITY IMMUNOASSAYS AND KITS FOR THE DETERMINATION OF PEPTIDES AND PROTEINS OF BIOLOGICAL INTEREST - The invention relates to immunoassays which allow the detection of polypeptides in samples with a higher sensitivity than assays of the state of the art. The invention also relates to kits which provide the components needed for carrying out said immunoassays. | 2009-02-05 |
20090035791 | Neural Colony Forming Assay - A neural colony forming cell (NCFC) assay is described. The assay allows one to distinguish neural stem cells from neural progenitor cells. In one embodiment, the present invention provides a method for identifying neural stem cells or neural progenitor cells comprising: (a) suspending neural cells in a semi-solid medium which supports the growth of neural cells; (b) plating the cells in the semi-solid medium at a density that allows for the production of colonies; (c) culturing the plated cells until size differences can be discerned between the colonies; and (d) estimating colony size wherein the larger colonies are likely produced by neural stem cells and wherein the small colonies are likely produced by neural progenitor cells In alternate embodiments, NSC can be distinguished from neural progenitor cells by determining the morphology or antigen expression of the colonies. | 2009-02-05 |
20090035792 | DRUG SELECTION FOR LUNG CANCER THERAPY USING ANTIBODY-BASED ARRAYS - The present invention provides compositions and methods for detecting the activation states of components of signal transduction pathways in tumor cells. Information on the activation states of components of signal transduction pathways derived from use of the invention can be used for cancer diagnosis, prognosis, and in the design of cancer treatments. | 2009-02-05 |
20090035793 | Microchip for cell response evaluation - In a microchip which enables cell cultivation and accurate cell count measurement, fine particles affixed with cells are trapped within a passage by making the minimum width of a solution and fine particle inlet into a cell culture portion larger than the maximum diameter of the fine particles, and making the width of an outlet smaller than the maximum diameter of the fine particles. | 2009-02-05 |
20090035794 | METHOD FOR DETECTING, SCREENING, AND/OR MONITORING CANCER IN AN INDIVIDUAL - The invention relates to a method for screening and/or detecting and/or monitoring a cancer in an individual, said method comprising determining a first parameter represented by the concentration of TIMP-1 in at least one excreta, e.g. saliva, from the individual. The invention provides a method that without the need to use a blood sample is suitable for facilitating the early diagnosis of a cancer, monitoring the recurrence of a cancer, and/or monitoring the status of a cancer or the effect of cancer treatment in an individual. | 2009-02-05 |
20090035795 | METHOD AND COMPOSITION FOR FORMING A UNIFORM LAYER ON A SUBSTRATE - A method of reducing thickness non-uniformity in a microjet-deposited solids layer is disclosed which comprises depositing onto a substrate by microjet deposition a composition containing a liquid vehicle, at least one reagent; and a polyol which is present at a concentration that enhances thickness uniformity of a solids layer, containing the reagent(s), which is formed when the microjet-deposited composition is dried. | 2009-02-05 |
20090035796 | Enzyme sensors including environmentally sensitive or fluorescent labels and uses thereof - Sensors for detecting enzyme activity are provided. The sensors include substrate modules having environmentally sensitive labels and detection modules whose binding to the substrate modules results in changes in signals from the environmentally sensitive labels or polypeptides or polypeptide substrates including environmentally sensitive or fluorescent labels. Compositions including substrate modules, polypeptides, or polypeptide substrates and nucleic acids encoding enzymes and/or detection modules are also described. Methods of assaying enzyme activity using sensors including environmentally sensitive or fluorescent labels are provided, as are related methods for screening for modulators of enzyme activity. | 2009-02-05 |
20090035797 | Detection of Disease Associated Proteolysis - Described herein are methods and techniques to study the “degradome”. The degradome of a specific protease is the complete product of the natural substrate repertoire of that enzyme in a cell, tissue or organism. The complete set of proteases that are expressed at a particular moment or circumstance by a cell, tissue or organism produces the collective degradome. Included in the methods described herein are approaches that allow the direct identification and characterization of degradome peptides from approx. 400 to approx. 12,000 Da. The methods of the invention avoid the inherent problems of studying the peptidome by focusing on specific or unique proteolytic cleavages that occur as a result of endogenous protease activity induced by specific diseases. Once characterized, the presence of, or change in level of, specific peptides of the degradome can be used, e.g., to identify specific peptides having elevated levels compared to a reference normal/or to correlate identified peptides with specific proteins and/or to identify protein fragmentation patterns (e.g., peptide ladders) and the specific protease(s) that brought them about and then correlate this information with the presence or absense of a specific disease or condition. Thus, the methods of the invention can be used, for example, to identify new diagnostic markers and/or therapeutic targets, as specific clinical diagnostic methods for individual patients and as methods of monitoring the progress of a therapeutic regimen for the treatment of a patient. | 2009-02-05 |
20090035798 | Methods for Reducing the Immunogenicity of Cytokines and Removal of Cell Surface Markers - The present invention provides means to assess the comparative allergenicity of proteases. In particular, the present invention provides means to qualitatively assess the potential for any protease to induce an allergic response in humans. In addition, the present invention provides means to select proteases with reduced allergenicity for use in various applications. | 2009-02-05 |
20090035799 | NOVEL TRYPSIN FAMILY SERINE PROTEASES - Two novel trypsin-family serine proteases specifically expressed in adult mouse testis (“Tespec PRO-1” and “Tespec PRO-2”), and a novel trypsin-family serine protease derived from mouse (“Tespec PRO-3”) have been isolated. Also, two novel trypsin-family serine proteases derived from human (“Tespec PRO-2” and “Tespec PRO-3”) have been isolated. It has been suggested that these proteins are involved in sperm differentiation and maturation, and sperm functions (e.g., fertilization). Therefore, these proteins are useful for development of novel therapeutics and diagnostics for infertility, as well as for development of novel contraceptives. | 2009-02-05 |
20090035800 | Novel Use of Fluorescence Resonance Energy Transfer - A novel use of Fluorescence Resonance Energy Transfer wherein a labelled protein comprising a Fluorescent energy donor label and at least one energy acceptor moiety capable of accepting energy from the donor label by Förster energy transfer is exposed to incident electromagnetic energy to excite the donor moiety and the fluorescence emission of the donor is measured. The or each energy acceptor moiety has a more and less active energy acceptor state and the level of quenching of donor fluorescence is indicative of this state. The energy acceptor moiety may be converted between its states by a redox reaction, optionally involving a partner redox protein. A novel system comprising the labelled protein, a redox partner protein, ‘a light source for imposing incident light at the excitation wavelength for the fluorescent label and a light detector capable of detecting the fluorescence emitted by the label may be used in biosensors and/or to monitor enzymatic turnover. | 2009-02-05 |
20090035801 | Twelve (12) protein biomarkers for diagnosis and early detection of breast cancer - The invention relates to 12 identified protein biomarkers for diagnosis, determination of disease severity, and therapeutic response monitoring of patients with breast cancer. The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of up to 12 protein biomarkers, and statistical analysis of the concentration of the protein biomarkers. | 2009-02-05 |
20090035802 | METHOD FOR DETECTION AND ENUMERATION OF CELL SURFACE MARKERS - Disclosed is a method for detecting and counting cell surface markers using a difference in fluorescence intensities. A fluorescent material having a relatively lower fluorescence intensity is conjugated with one marker and another fluorescent material having a relatively higher fluorescence intensity is conjugated with the other marker. The two markers can be sorted and counted individually and simultaneously from the fluorescence intensity difference. | 2009-02-05 |
20090035803 | METHOD FOR STABILIZATION OF PEPTIDES IN A BIOLOGICAL SAMPLE - The present invention provides a simple method for the stabilization of a peptide in a biological sample and a reagent for the stabilization, a simple method for the preservation of a biological sample comprising a peptide and a reagent for the preservation, and a method for the accurate measurement of a peptide in a biological sample and a reagent for the measurement. Addition of a saccharide to a biological sample enables the stabilization of a peptide in the biological sample, the preservation of the biological sample comprising a peptide in a stable condition and the accurate measurement of a peptide in the biological sample. As the present invention can stabilize a peptide in a biological sample collected in the clinical examination, the peptide as a marker in the biological sample can be measured accurately in the clinical examination. | 2009-02-05 |
20090035804 | CONSTITUTIVELY OPEN hERG (Kv11.1) MUTANT CHANNELS - We disclose a cell having double mutations of the HERG gene that lead to charge reversal amino acid substitutions at residues 466 and 534 of the wild type Kv11.1 channel protein. These double charge reversal mutations result in cells having constitutively open Kv11.1 channels. Such cells could be used in a method of testing development-stage drugs and other compounds for Kv11.1 channel block activity. | 2009-02-05 |
20090035805 | C3A Serum-Free Clonal Cell Line and Methods of Use - A serum-free C3A clonal cell line and methods for generating the same are provided. The C3A cell line has a reduced doubling time in serum-free medium compared to a corresponding C3A cell line from which it is derived. Methods using the cells of the serum-free C3A clonal cell line for the production, expression and recovery of harvestable polypeptides, screening compounds for metabolic activity, studying enteric disease and for use in a bio-artificial liver device are also provided. | 2009-02-05 |
20090035806 | Method for Identification of Age of an Ancient Ware by Using Microbes - This invention is related to a method for identification of age of the ancient wares, particularly a method for identification of age of the ancient wares by using the microbe. The method of this invention includes the following steps: establish database corresponding to the microbe parameters in wares to be measured; take sample from the wares to be measured; measure the microbe parameters value; compare the obtained parameters value with the standard database to obtain the ware production year. This invention has features of correct and reliable identification of age of the ancient wares, and wide application range. | 2009-02-05 |
20090035807 | ANALYSIS OF POLYPEPTIDE PRODUCTION - The present invention is directed to methods of evaluating a process to produce a polypeptide preparation or evaluating an element of a process to produce a polypeptide preparation. The methods comprise providing a sample comprising the polypeptide, wherein the sample has not been subjected to affinity chromatography, and subjecting the sample to liquid chromatography. The sample may be a process bioreactor sample. The methods of the invention may be used to determine a value of a characteristic of the sample. The methods of the invention provide a real time or near real-time information regarding the process to produce a polypeptide. | 2009-02-05 |
20090035808 | MEDIUM FOR DETECTING MICROORGANISMS - Provided is a culture medium for microorganisms present in contaminated working fluids such as coolants. More particularly, said culture medium is particularly suitable for supporting growth of microorganisms colonizing metalworking fluids and allows for specific detection of both bacterial microorganisms and fungal microorganisms the latter depending on the added selective agents which can be antibiotics for the detection of fungal contamination or fungicides for the detection of bacterial contamination. Furthermore, devices and kits comprising the culture medium of the present invention are described as well as a method of detecting microbial contamination of metalworking fluids. | 2009-02-05 |
20090035809 | Modified Carbocyanine Dyes and Their Conjugates - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens. | 2009-02-05 |
20090035810 | MODIFIED CARBOCYANINE DYES AND THEIR CONJUGATES - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens. | 2009-02-05 |
20090035811 | Artificial scaffolding material for protein retention and use of the same - The present invention provides an artificial scaffolding material for retaining proteins suitable for placing contiguously one species or two or more species of proteins such as enzymes. To this end, the artificial scaffolding material for retaining proteins is provided with a cell and scaffolding proteins heterologous to the cell and placed on the surface layer side of the cell at an extent that allows aggregation properties to be conferred to the cell, and provided with a plurality of non-covalently binding protein-binding domains arranged in tandem. | 2009-02-05 |
20090035812 | Microbial hydrogen-producing process and system thereof - The present invention provides a microbial hydrogen-producing process, comprising: providing at least one | 2009-02-05 |
20090035813 | OLIGOSACCHARIDE MIXTURE - An oligosaccharide mixture derived from animal milk, food products comprising said oligosaccharide mixture and a process for producing said oligosaccharide mixture. | 2009-02-05 |
20090035814 | Method for producing circular or multimeric protein species in vivo or in vitro and related methods - A method is disclosed for forming multimeric proteins. The method relies on intermolecular trans-splicing of a split intein either in vivo or in vitro. | 2009-02-05 |
20090035815 | Synthetic Gene for Enhanced Expression in E. Coli - A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for | 2009-02-05 |
20090035816 | Process for the preparation of a polypeptide - A process for the preparation of a polypeptide made from amino acids L-alanine, L-glutamic acid, L-lysine, and L-tyrosine comprising using N-thiocarboxyanhydride of at least one amino acid as a starting material. | 2009-02-05 |
20090035817 | Method for Preparing Transformants Expressing Benzaldehyde Dehydrogenase and Preparation of 2,6-Naphthalene Dicarboxylic Acid Using the Transformants - Disclosed herein are a method for preparing a transformant which carries a gene encoding benzaldehyde dehydrogenase derived from | 2009-02-05 |
20090035818 | Novel Polypeptide, Polynucleotide Encoding the Polypeptide and Use the Polypeptide and Polynucleotide - In one embodiment of the present application, a polypeptide capable of binding to a sugar chain is disclosed, particularly a high-mannose-type sugar chain bound to an antibody, more preferably a sugar chain bound to a chicken antibody. Also disclosed is a method for the purification of an antibody (specifically a chicken antibody) as a representative application of the polypeptide. Further disclosed is means for the purification. The polypeptide, BML-17, is a novel lectin made of 168 amino acid residues isolated from | 2009-02-05 |
20090035819 | CA125 gene and its use for diagnostic and therapeutic interventions - The CA125 gene has been cloned and multiple repeat sequences as well as the carboxy terminus have been identified. The CA125 molecule comprises three major domains: an extracellular amino terminal domain (Domain 1); a large multiple repeat domain (Domain 2); and a carboxy terminal domain (Domain 3) which includes a transmembrane anchor with a short cytoplasmic domain. The amino terminal domain is assembled by combining five genomic exons, four very short amino terminal sequences and one extraordinarily large exon. This domain is dominated by its capacity for O-glycosylation and its resultant richness in serine and threonine residues. Additionally, an amino terminal extension is present, which comprises four genomic exons. The amino acid composition of the amino terminal extension was found to be consistent with the amino acid composition of the amino terminal domain. The molecular structure is dominated by a repeat domain comprising 156 amino acid repeat units, which encompass the epitope binding sites. More than 60 repeat units have been identified, sequenced, and contiguously placed in the CA125 domain structure. The repeat units encompass an interactive disulfide bridged C-enclosure and the site of OC125 and M11 binding. The repeat sequences demonstrated 70-85% homology to each other. Expression of the repeats was demonstrated in | 2009-02-05 |
20090035820 | Microbial Trypsin Mutants Having Chymotrypsin Activity And Nucleic Acids Encoding Same - The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof. | 2009-02-05 |
20090035821 | Expression of Proteins in E.Coli - Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX | 2009-02-05 |
20090035822 | Fusion Proteins - A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that is capable of binding to a Binding Site on the nociceptive sensory afferent, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent. Nucleic acid sequences encoding the polypeptide fusion proteins, methods of preparing same and uses thereof are also described. | 2009-02-05 |
20090035823 | Ligation-based synthesis of oligonucleotides with block structure - The present invention relates to a method of producing single-stranded nucleic acid molecules from oligo- or polynucleotides wherein each of said oligo- or polynucleotides has a predefined 5′ or 3′ terminus, comprising the steps of (a) annealing an adaptor oligonucleotide simultaneously or step by step to (aa) a first oligo- or polynucleotide; and (ab) a second oligo- or polynucleotide wherein the 5′-terminus of said adaptor oligonucleotide is complementary in sequence to the 5′ terminus of said first oligo- or polynucleotide and the 3′terminus of said adaptor molecule is complementary in sequence to the 3′ terminus of said second oligo- or polynucleotide; and optionally (a′) simultaneously with or subsequently to step (a) annealing at least one further adaptor oligonucleotide to free termini of said first or second oligonucleotides and to free termini of further oligo- or polynucleotides; (b) optionally filling in gaps between the neighbouring ends of said oligo- or polynucleotides; (c) ligating said oligo- or polynucleotides; and (d) removing said at least one adaptor oligonucleotide. In a preferred embodiment of the method of the invention, said single-stranded nucleic acid molecules represent a collection of nucleic acid molecules wherein either said first or said second oligo- or polynucleotide is invariable in sequence between all members of said collection of nucleic acid molecules. | 2009-02-05 |
20090035824 | NUCLEIC ACID-TEMPLATED CHEMISTRY IN ORGANIC SOLVENTS - The present invention provides methods and compositions for performing nucleic acid mediated chemistry in a variety of organic solvents. A variety of nucleic acid mediated chemical reactions may be efficiently carried out in organic solvents. | 2009-02-05 |
20090035825 | Devices, Systems, and Methods for Preparing Emulsions - A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously. | 2009-02-05 |
20090035826 | METHOD FOR THE PRODUCTION OF ALCOHOL FROM A PRETREATED LIGNOCELLULOSIC FEEDSTOCK - A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose. | 2009-02-05 |
20090035827 | O-acetyltransferase from Neisseria Meningitidis, Compositions and Methods - Provided are recombinant DNA molecules that do not occur in nature encoding a Lot3 O-acetyltransferase, vectors that direct expression of a Lot O-acetyltransferase, recombinant host cells which express a Lot3 O-acetyltransferase, methods for recombinant production of a Lot3 O-acetyltransferase, methods for acetylating lipooligosaccharides, especially those of a | 2009-02-05 |
20090035828 | Method For the Preparation of Cross Linked Protein Crystals - A protein such as an enzyme is immobilized by crosslinking crystals of the protein with a multifunctional crosslinking agent. The crosslinked protein crystals may be lyophilized for storage. A preferred protein is an enzyme such as amyloglucosidase, Horse radish peroxidase, plant peroxidases etc. Crosslinked enzyme crystals preferably retain at least 90% activity after incubation for three hours in the presence of a concentration of protease that causes the soluble uncrosslinked form of the enzyme to lose at least 92% of its initial activity under the same conditions. Enzyme crystals that are crosslinked may be microcrystals having a cross-section of 100 microns or less. Crosslinked enzyme crystals are sturdy and can withstand harsh conditions and may be used for performing selective chemical reactions in organic or aqueous medium, in an assay, diagnostic kit or biosensor for detecting an analyte, in producing a product such as using crosslinked Peroxidase crystals to produce novel polymers, biotransformations including those used in industrial scale chemical processes and in environmental remediations. | 2009-02-05 |
20090035829 | Enzymatic process for the preparation of paliperidone and its intermediate CMHTP - The present invention provides a process for the preparation of a 4H-pyrrido[1,2-a]-pyrimidin-4-one derivative, wherein the 4H-pyrrido[1,2-a]-pyrimidin-4-one derivative is Paliperidone or 3-(2-chloroethyl)-6,7,8,9-tetrahydro-9-hydroxy-2-methyl-4H-pyrrido[1,2-a]-pyrimidin-4-one (‘CMHTP’), said process comprising enzymatically hydroxylating Risperidone or 3-(2-chloroethyl)-6,7,8,9-tetrahydro-2-methyl-4H-pyrrido[1,2-a]-pyrimidin-4-one (‘ClMTTP’), respectively, with at least one oxidoreductase enzyme; and optionally isolating or purifying the Paliperidone or CMHTP, wherein the at least one oxidoreductase enzyme is selected from the group of peroxidases, dioxygenases, monooxygenases and any combination thereof. | 2009-02-05 |
20090035830 | Method for Preparing Optically Active Amines - The present invention relates to a method of preparing optically active amines and chiral amines prepared thereby. The method includes reacting an amine compound, a metal catalyst, a biocatalyst including a lipase, and an acyl donor compound in an organic solvent to obtain a chiral amide compound, and then hydrolyzing the chiral amide compound to obtain a chiral amine. | 2009-02-05 |
20090035831 | Enantioselective immobilized lipase - Immobilization of | 2009-02-05 |
20090035832 | Methods and compositions for production of methane gas - The present invention provides methods and compositions for sustained methane production from atmospheric CO | 2009-02-05 |
20090035833 | Method for recovering metal values from refractory sulfide ore - A method for recovering metal values from refractory sulfide ores is provided. The method includes the steps of separating clays and fines from crushed refractory sulfide ore, forming a heap from the refractory sulfide ore, producing a concentrate of refractory sulfide minerals from the separated fines and adding the concentrate to the heap, bioleaching the heap to thereby oxidize iron sulfides contained therein, and hydrometallurgically treating the bioleached ore to recover metal values contained therein. | 2009-02-05 |
20090035834 | Method for Using Biomass in Biogas Process - The invention relates to a method for using biomass in a biogas process. The aim of the invention is to use substrates having a high nitrogen and solid content, and which using a small amount of water, has very good energy balance and is particularly environmentally friendly. Said aim is achieved by the virtue of the fact that the substrate is treated with a recirculated product in order to form a pumpable medium, and additionally treated with bacteria in cyclones and fermenters, which simultaneously removes the nitrogen in a stripping process, separates the solid fermentation radicals and further uses the recirculated product as a heat-exchanger and reaction medium. Said process is environmentally friendly and has a very good energy balance. | 2009-02-05 |
20090035835 | Method and device for producing biomass of photosynthesizing microorganisms/phototrophical algae and biomass of these microorganisms pigments - The invention relates to biotechnology, in particular to methods and means of physical action on biological structures of photosynthesing microorganisms, phototrophic algae in particular. The invention can be used in pharmaceutical, cosmetic and foodstuff industries, as well as for obtaining biofuel from algae In the process of the method implementation radiation of cultivated solution of photosynthesizing microorganisms/phototrophical algae is carried out by the action of electromagnetic waves of mm range and low intensity. Stimulation of increasing photosynthesizing microorganisms/phototrophical algae biomass and biomass of their pigments (excretions) is obtained with industrial production as a result of the resonance effect which is caused by the interaction of electromagnetic wave and biological cell. Irradiation of cultivated solution of photosynthesizing microorganisms/phototrophical algae is performed by electromagnetic waves of mm range and low intensity at different phases of cultured biological objects development. | 2009-02-05 |
20090035836 | MODULATING PH-SENSITIVE BINDING USING NON-NATURAL AMINO ACIDS - The invention provides methods, systems and reagents for regulating pH-sensitive protein interaction by incorporating non-natural amino acids into the protein (e.g. an antibody, or its functional fragment, derivative, etc.). The invention also relates to specific uses in regulating pH-sensitive binding of antibodies to tumor site, by conferring enhanced tumor-specificity/selectivity. In that embodiment, the non-natural amino acids preferably have desirable side-chain pKa's, such that at below physiological pH (e.g. about pH 6.3-6.5) the non-natural amino acid confer enhanced binding to tumor antigens in acidic environments. Such non-natural amino acids can be incorporated by any suitable means, such as by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs (e.g. the degenerate codon orthogonal system. | 2009-02-05 |
20090035837 | Virus Production - An improved process for recovery of virus from allantoic fluid of virus-infected chick embryos. Virus associated with granular and fibrous debris in the allantoic fluid can be disassociated from the debris and recovered, thereby increasing viral yield. Dissociation can be achieved by subjecting the virus-debris complex to conditions of increased salt concentrations, e.g., 0.5 M or greater. | 2009-02-05 |
20090035838 | Microfabricated Crossflow Devices and Methods - A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly. | 2009-02-05 |
20090035839 | Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof - A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including | 2009-02-05 |
20090035840 | THERMACETOGENIUM PHAEUM CONSORTIUM FOR THE PRODUCTION OF MATERIALS WITH ENHANCED HYDROGEN CONTENT - An isolated microbial consortium is described that includes a first microbial consortium having | 2009-02-05 |
20090035841 | Enzymatic Production Of Glycolic Acid - Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of | 2009-02-05 |
20090035842 | Sucrose Feedstock Utilization for Oil-Based Fuel Manufacturing - The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar beets, and cane/beet agricultural processing byproducts as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a conversion technology to convert chemical energy produced by sugar cane and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oil-bearing microbes containing one or more exogenous sucrose utilization genes. | 2009-02-05 |
20090035843 | LIQUID MYCORRHIZA COMPOSITIONS - The present invention relates to liquid mycorrhiza compositions and to methods for colonizing a plant, grass, tree or shrub with one or more mycorrhizas. | 2009-02-05 |
20090035844 | Mechanical cell wounder device and related method - Disclosed is a device and related method for biological manipulation. With greater particularity, disclosed is a novel device and method for uniform cellular manipulation with economies of scale and operational uniformity. The device and related method utilize a cellular wounder ( | 2009-02-05 |
20090035845 | Method and device for extracting and/or collecting blood from placenta and/or umbilical cord - A device for extracting and collecting blood from a delivered placenta comprising a compartment to contain and support a delivered placenta and umbilical cord. The compartment includes a flexible membrane which can be displaced (preferably under the influence of a fluid (preferably gas) pressure differential between the compartment side and non compartment side of the membrane) to impart onto the placenta a pressure to encourage the displacement of fluid carried by the placenta towards the umbilical cord. The compartment includes at least one outlet opening via which the umbilical cord can extend (preferably in a sealed manner) to allow the flexible membrane pressure induced flow of fluid carried by the placenta to displace from the compartment for external to the compartment collection of the fluid. | 2009-02-05 |
20090035846 | DEVICE FOR MEASURING EXTRACELLULAR POTENTIAL AND METHOD OF MANUFACTURING DEVICE - A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily. | 2009-02-05 |
20090035847 | CENTRIFUGAL FORCE-BASED MICROFLUIDIC DEVICE FOR NUCLEIC ACID DETECTION AND MICROFLUIDIC SYSTEM INCLUDING THE SAME - Provided are a centrifugal force-based microfluidic device for separating and amplifying a nucleic acid of a target cell and a microfluidic system including the same. The microfluidic device includes a rotary platform; a target cell nucleic acid extraction unit; and a polymerase chain reaction (PCR) unit wherein in a microfluidic structure arranged in the platform and connected to the target cell nucleic acid extraction unit. | 2009-02-05 |
20090035848 | MOVING BED BIOFILM REACTOR (MBBR) SYSTEM FOR CONVERSION OF SYNGAS COMPONENTS TO LIQUID PRODUCTS - A moving bed bioreactor (MBBR) produces liquid products from a gaseous substrate of CO and/or CO | 2009-02-05 |
20090035849 | Depletion of plasma proteins - This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention. | 2009-02-05 |
20090035850 | P49/STRAP is a novel protein involved in gene regulation and cell proliferation - The invention provides isolated p49/STRAP protein, and isolated nucleic acids encoding a p49/STRAP protein. The inventors have discovered a new protein, named p49/STRAP that is expressed in cardiac tissue and other tissues in mammals. The p49/STRAP protein binds to serum response factor (SRF) and regulates transcription of SRF-responsive genes in the heart. p49/STRAP is also discovered to inhibit tumor cell proliferation, and thus the invention provides a method of inhibiting cancer cell proliferation by contacting the cells with p49/STRAP. | 2009-02-05 |
20090035851 | Modulators Of NOD1 Signaling - The present invention relates to intracellular signaling molecules, in particular the Nod1 protein. The present invention provides methods of identifying modulators of Nod1 signaling. The present invention further provides methods of altering Nod1 signaling. | 2009-02-05 |
20090035852 | Stable Atomic Quantum Clusters, Production Method Thereof and Use of Same - Stable atomic quantum clusters, AQCs, characterized by being composed of at least 500 metal atoms, its production process characterized by having a kinetic control and by maintaining a low concentration of reagents in the reaction medium, as well as the uses of these clusters as sensors (fluorescent, magnetic or chemical), electrocatalysts and as cytostatics and/or cytotoxics. | 2009-02-05 |
20090035853 | TSG101-GAG INTERACTION AND USE THEREOF - Isolated protein complexes are provided comprising Tsg101 and HIV GAG or GAGp6. The protein complexes are useful in screening assays for selecting compounds effective in modulating the Tsg101-HIV GAG or GAGp6 interaction within the protein complexes. | 2009-02-05 |
20090035854 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA - The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery. | 2009-02-05 |
20090035855 | THREE-DIMENSIONAL RECONSTITUTED EXTRACELLULAR MATRICES AS SCAFFOLDS FOR TISSUE ENGINEERING - A biomaterial scaffold comprising: a) reconstituted extracellular matrix; and b) polyelectrolyte complex fibers; wherein the matrix and the fibers are functionally associated. | 2009-02-05 |
20090035856 | CONTINUOUS PERFUSION BIOREACTOR SYSTEM - Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein. | 2009-02-05 |
20090035857 | CELL CULTURE PROCESSING DEVICES AND METHODS - Embodiments are directed to devices and methods for processing, cultivating or otherwise manipulating cell cultures which may be disposed on a flat or substantially flat surface such as cell culture substrate material. Devices and methods are disclosed for dividing a cell culture layer into divided portions, including isolated divided portions, that may then be transferred from the cell culture to a new location. For some embodiments, the divided portions may be transferred to a new cell culture support substrate in order to continue to grow and cultivate the cell line. | 2009-02-05 |
20090035858 | Tissue engineering support - Cell cultures or tissue engineering supports, include at least a porous matrix based on a collagen sponge which defines first pores and a porous three-dimensional knit which defines second pores, the porous matrix filling the three-dimensional knit and all the first and second pores being at least partially interconnected with one another. | 2009-02-05 |
20090035859 | METHOD AND DEVICES FOR PREVENTING RESTENOSIS IN CARDIOVASCULAR STENTS - Described herein are devices and methods fabricating devices having nanostructures that allow adhesion or growth of one cell type, such as endothelial cells, more than another cell type, such as smooth muscle cells. In particular, stent covers having such nanostructures are described, and methods for fabricating these stent covers. Also described herein are methods for optimizing the nanostructures forming the devices. | 2009-02-05 |
20090035860 | STEM CELL GROWTH MEDIA AND METHODS OF MAKING AND USING SAME - The invention provides media formulations. A complete media formulation of the invention includes, for example, the following components: albumin, an iron carrier, glutamine, a glycosidase or hydrolase, fibroblast growth factor (FGF), a salt or mineral, and essential amino acids, at an osmolarity of about 220-330 mOsm/Liter. | 2009-02-05 |
20090035861 | RNAi Medicine Having No Adverse Effects - An RNAi reagent and a medicine that have no adverse effects such as interferon and/or cytotoxicity induction are provided. | 2009-02-05 |
20090035862 | METHODS AND COMPOSITIONS FOR GENOMIC MODIFICATION - The present invention provides methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell, as well as, enzymes, polypeptides, and a variety of vector constructs useful therefore. In the method, a targeting construct comprises, for example, (i) a first recombination site and a polynucleotide sequence of interest, and (ii) a site-specific recombinase, which are introduced into the cell. The genome of the cell comprises a second recombination site. Recombination between the first and second recombination sites is facilitated by the site-specific recombinase. The invention describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors, and methods of the present invention are also useful in gene therapy techniques. | 2009-02-05 |
20090035863 | Method for the deletion of a large chromosomal region - In order to efficiently delete a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding a toxic substance such as Aflatoxin, the present invention provides a method for the deletion of a large chromosomal region comprising transforming a transformant having an increased frequency of homologous recombination due to suppression of a Ku gene, which is a mitosporic filamentous fungus belonging to Trichocomaceae with a vector for the deletion of a chromosomal region, which comprises a pair of homologous region arms having a nucleotide sequence that is homologous with a fragment at either end of said chromosomal region, and deleting the chromosomal region by means of homologous recombination between the resulting transformant and said vector. | 2009-02-05 |
20090035864 | PLASMIDS FOR TRANSFORMING BACTERIA OF THE ACIDITHIOBACILLUS SPP. GENUS, AND TRANSFORMATION METHOD - The present invention discloses functional plasmids in bacteria of the | 2009-02-05 |
20090035865 | MOISTURE SENSOR - A moisture sensor is disclosed which measures cumulative exposure to moisture. The moisture sensor comprises a matrix having a hygroscopic material, a first reagent, and a second reagent which interact only in the presence of water to produce a detectable, irreversible change in the matrix to provide a moisture indication. The moisture sensor may be incorporated with a disposable diagnostic device, whereby a method for preventing the use of such a disposable diagnostic device if exposed to excessive cumulative humidity is also disclosed. | 2009-02-05 |