07th week of 2019 patent applcation highlights part 27 |
Patent application number | Title | Published |
20190048307 | AGRICULTURAL ADMIXTURES - Methods and systems for manipulating varied biological recyclable streams to produce agricultural admixtures are herein described. The resulting agricultural admixtures can be used to enhance crop yield, or as an animal provender. Managing the sources of varied biological recyclable streams can afford agricultural admixtures with controlled properties. | 2019-02-14 |
20190048308 | GAS-FED FERMENTATION REACTORS, SYSTEMS AND PROCESSES UTILIZING GAS/LIQUID SEPARATION VESSELS - Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described. | 2019-02-14 |
20190048309 | CELL LYSIS - In an example implementation, a method of cell lysis includes moving cell fluid from a first reservoir through a microfluidic channel toward a second reservoir, activating a lysing element multiple times as a cell from the cell fluid passes through the microfluidic channel, and moving lysate fluid that results from the activating through the microfluidic channel and into the second reservoir. | 2019-02-14 |
20190048310 | SYNTHETIC METHYLOTROPHY TO LIQUID FUELS AND CHEMICALS - A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided. | 2019-02-14 |
20190048311 | SPORULATION METHOD OF BACILLUS BACTERIUM - A method of producing | 2019-02-14 |
20190048312 | Dry Powder Cell Culture Products and Methods of Production Thereof - The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. | 2019-02-14 |
20190048313 | STABILIZED AMORPHOUS CALCIUM CARBONATE AS A SUPPLEMENT FOR CELL CULTURE MEDIA - Stabilized amorphous calcium carbonate (ACC) as a supplement of cell culture media and the cell culture medium supplements comprising stabilized ACC are provided. In particular the stabilized ACC is useful for enhancing the growth of cell and tissue cultures, gametes and embryos in vitro. | 2019-02-14 |
20190048314 | Modulation of Angiogenesis - The invention provides methods for treating pathological conditions that can be improved by providing angiogenesis. The invention is generally directed to provide angiogenesis by administering cells that express and/or secrete one or more pro-angiogenic factors. The invention is also directed to drug discovery methods to screen for agents that modulate the ability of the cells to express and/or secrete one or more pro-angiogenic factors. The invention is also directed to cell banks that can be used to provide cells for administration to a subject, the banks comprising cells having desired levels of expression and/or secretion of one or more pro-angiogenic factors. | 2019-02-14 |
20190048315 | STEM CELL-DERIVED HEPATOCYTES IN CO-CULTURE AND USES THEREOF - The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture. | 2019-02-14 |
20190048316 | IMPROVED BLOOD-BRAIN BARRIER ENDOTHELIAL CELLS DERIVED FROM PLURIPOTENT STEM CELLS FOR BLOOD-BRAIN BARRIER MODELS - The invention relates to a method of creating a human blood-brain barrier (BBB) model from the differentiation of human pluripotent stem cells (hPSCs), wherein the BBB exhibits sustained transendothelial electrical resistances (TEER) over 2000 Ω·cm | 2019-02-14 |
20190048317 | Method for Producing Highly Functional Platelets - The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors. | 2019-02-14 |
20190048318 | LITHIUM DISILICATE GLASS-CERAMIC COMPOSITIONS AND METHODS THEREOF - A bioactive glass-ceramic composition as defined herein. Also disclosed are methods of making and using the disclosed compositions. | 2019-02-14 |
20190048319 | METHODS FOR GENERATING CARDIOMYOCYTES - The present disclosure provides method of generating cardiomyocytes from post-natal fibroblasts. The present disclosure further provides cells and compositions for use in generating cardiomyocytes. | 2019-02-14 |
20190048320 | Method For Promoting Differentiation Of Pluripotent Stem Cells By Reducing Undifferentiated State Thereof - In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed. | 2019-02-14 |
20190048321 | Macrophage Enrichment of Cancer Stem Cells - Disclosed are culture systems and methods that can enrich a cancer cell population in cancer stem cells. The culture system can be utilized for study of cancer stem cells in general as well as for cancer drug screening. The methods and systems include co-culturing of a cancer cell population and programable macrophages, which can lead to proliferation of the cancer stem cells of the population and depletion of differentiated cancer cells of the population. The methods and systems can provide a more reliable and accurate model for preclinical drug toxicity testing. | 2019-02-14 |
20190048322 | METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS PRODUCED THEREBY - According to one aspect of the present invention, there is provided a method for preparing customized pluripotent stem cells and induced pluripotent stem cells thereby. According to this method, a large number of induced pluripotent stem cells can be obtained in high yield. In addition, the induced pluripotent stem cells produced thereby can be used in various fields such as cell therapeutic agents and cosmetic composition. | 2019-02-14 |
20190048323 | BIOLOGICAL CONTROL OF PLANT VIRUSES - The present disclosure provides new attenuated | 2019-02-14 |
20190048324 | HIGH TITER RECOMBINANT INFLUENZA VIRUSES FOR VACCINES - The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes at least five internal genes from an influenza virus isolate that replicates to high titers in embryonated chicken eggs or MDCK cells. | 2019-02-14 |
20190048325 | BACTERIOPHAGE ENGINEERING VIA SEMI-SYNTHESIS - The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence. | 2019-02-14 |
20190048326 | VARIANT POLYPEPTIDES CAPABLE OF AMINATING ALIPHATIC ALPHA KETO ACIDS - Disclosed are, among other things, variant polypeptides, nucleic acids encoding the polypeptides, production of the variant polypeptides, and use of the variant polypeptides in various applications, such as screening and synthetic methods. For example, the variant polypeptides, or enzymatically-active fragments thereof, are useful for converting aliphatic keto acids to aliphatic alpha amino acids. | 2019-02-14 |
20190048327 | URICASE SEQUENCES AND METHODS OF TREATMENT - Described are improved uricase sequences having beneficial effects and methods of treating patients suffering from hyperuricemia. | 2019-02-14 |
20190048328 | GLYCOSYLTRANSFERASE GLYCOSYLATING FLAVOKERMESIC ACID AND/OR KERMESIC ACID - An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid. | 2019-02-14 |
20190048329 | ENZYMES AND METHODS FOR PROCESSING LIGNIN AND OTHER AROMATIC COMPOUNDS - Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, β-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The β-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, | 2019-02-14 |
20190048330 | COMPOSITIONS AND METHODS FOR REGULATING GENE EXPRESSION FOR TARGETED MUTAGENESIS - Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene. | 2019-02-14 |
20190048331 | BETA-LACTAMASE VARIANTS - The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability. | 2019-02-14 |
20190048332 | Contiguity Preserving Transposition - Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid. | 2019-02-14 |
20190048333 | TCR LIBRARIES - The present invention relates to a library of particles, the library displaying a plurality of different T cell receptors (TCRs), wherein the plurality of TCRs consists essentially of TCRs comprising an alpha chain comprising an alpha chain variable domain and a beta chain comprising a beta chain variable domain and the library comprises more than one TRAY gene product and/or more than one TRBV gene product, wherein the beta chain variable domain does not comprise one or more of a TRBV5-1, 5-3, 5-4, 5-5, 5-6, 5-7 or 5-8 gene product and wherein the plurality of TCRs do not consist essentially of TCRs comprising a TRAV12-2 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire and TCRs comprising a TRAV21 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire. | 2019-02-14 |
20190048334 | METHODS FOR SAMPLE PREPARATION - The disclosure provides for single amplification and double amplification methods for preparing nucleic acid samples for sequencing. | 2019-02-14 |
20190048335 | IMPROVED AMPLIFICATION AND SEQUENCING METHODS - In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, and apparatuses for any one or any combination of: conducting a library preparation method which generates a mixture of desirable template polynucleotides and non-desirable polynucleotide byproducts, amplifying the resulting library, enriching the desirable template polynucleotides, and sequencing the enriched template polynucleotides. The methods, as well as related systems, compositions, kits, and apparatuses, of the present teachings can be used to improve sequencing data. | 2019-02-14 |
20190048336 | COMPOSITIONS AND METHODS FOR CONSTRUCTING STRAND SPECIFIC CDNA LIBRARIES - Provided herein are compositions, kits and methods for the production of strand-specific cDNA libraries. The compositions, kits and methods utilize properties of double stranded polynucleotides, such as RNA-cDNA duplexes to capture and incorporate a novel sequencing adapter. The methods are useful transcriptome profiling by massive parallel sequence, such as full-length RNA sequencing (RNA-Seq) and 3′ tag digital gene expression (DGE). | 2019-02-14 |
20190048337 | Crispr/Cas-Related Methods and Compositions for Treating Duchenne Muscular Dystrophy and Becker - CRISPR/CAS-related compositions and methods for treatment of DMD, BMD, or DCM type 3B are described. | 2019-02-14 |
20190048338 | STRUCTURE-GUIDED CHEMICAL MODIFICATION OF GUIDE RNA AND ITS APPLICATIONS - The disclosure relates to compositions comprising and methods for chemical modification of single guide RNA (sgRNA), tracrRNA and/or crRNA used individually or in combination with one another or Cas system components. Compositions comprising modified ribonucleic acids have been designed with chemical modification for even higher efficiency as unmodified native strand of sgRNA. Administration of modified ribonucleic acids will allow decreased immune response when administered to a subject, increased stability, increased editing efficiency and facilitated in vivo delivery of sgRNA via various delivery platforms. The disclosure also relates to methods of decreasing off-target effect of CRISPR and a CRISPR complex. | 2019-02-14 |
20190048339 | METHODS FOR REACTIVATING GENES ON THE INACTIVE X CHROMOSOME - Methods for reactivating genes on the inactive X chromosome that include administering one or both of a DNA methyltransferase (DNMT) Inhibitor and/or a topoisomerase inhibitor, e.g., etoposide and/or 5-azacytidine (aza), optionally in combination with an inhibitor of XIST RNA and/or an Xist-interacting protein, e.g., a chromatin-modifying protein, e.g., a small molecule or an inhibitory nucleic acid (such as a small inhibitory RNA (siRNAs) or antisense oligonucleotide (ASO)) that targets XIST RNA and/or a gene encoding an Xist-interacting protein, e.g., a chromatin-modifying protein. | 2019-02-14 |
20190048340 | NOVEL FAMILY OF RNA-PROGRAMMABLE ENDONUCLEASES AND THEIR USES IN GENOME EDITING AND OTHER APPLICATIONS - A new family of RNA-programmable endonucleases, associated guide RNAs and target sequences, and their uses in genome editing and other applications are disclosed herein. | 2019-02-14 |
20190048341 | REDUCED SIZE SELF-DELIVERING NUCLEIC ACID COMPOUNDS TARGETING LONG NON-CODING RNA - The present disclosure relates to RNAi constructs with improved cellular uptake characteristics and methods of use of these compounds for silencing expression of long coding RNAs (IncRNAs). | 2019-02-14 |
20190048342 | CROSS-REGULATION OF TYPE I INTERFERON SIGNALING PATHWAYS - Compositions and methods for cross-regulation of type I interferon signaling pathways in pDCs for vaccine development are provided. | 2019-02-14 |
20190048343 | ANTI-ANGIOGENIC MIRNA THERAPEUTICS FOR INHIBITING CORNEAL NEOVASCULARIZATION - The disclosure relates, in some aspects, to compositions and methods for treating corneal disease (e.g., corneal neovascularization. In some embodiments, the disclosure relates to rAAV-mediated delivery of an cornea-associated transgene to a subject. In some embodiments, the rAAV transduces the corneal tissue of a subject. | 2019-02-14 |
20190048344 | CHEMICAL MODIFICATIONS OF MONOMERS AND OLIGONUCLEOTIDES WITH CYCLOADDITION - The invention features compounds of formula I or II: | 2019-02-14 |
20190048345 | Post-Transcriptionally Chemically Modified Double Strand RNAs - Described are post transcriptionally chemically modified double strand RNAs (MdsRNAs) having more than 30 base pairs. The MdsRNAs inhibit gene expression in target organisms. Also described are methods of making and using MdsRNAs. | 2019-02-14 |
20190048346 | COMPOSITIONS AND METHODS FOR DELIVERING MICRORNA - The invention relates to compositions, methods and kits for using Argonaute-2 (Ago-2) as a systemic carrier to deliver a miRNA to an endothelial cell. The invention also relates to compositions, methods and kits for inhibiting angiogenesis and/or treating a condition by using Ago-2 as a systemic carrier to deliver a miRNA to an endothelial cell. The condition includes but is not limited to brain vascular diseases and brain tumors. | 2019-02-14 |
20190048347 | NUCLEIC ACID APTAMER AS1411 MODIFIED DNA TETRAHEDRON AND PREPARATION METHOD THEREOF - This invention discloses a nucleic acid aptamer AS1411 modified DNA tetrahedron. The preparation method includes the steps of (1) binding an AS1411 sequence to the 5′ terminal of any DNA single-strand in a DNA tetrahedron, synthesizing the DNA, dissolving obtained DNA powder with ddH | 2019-02-14 |
20190048348 | Aptamers For Consumer Product Compositions - Consumer product compositions comprise a surfactant and a nucleic acid aptamer. The nucleic acid aptamer comprises at least one oligonucleotide comprising: deoxyribonucleotides, ribonucleotides, derivatives of deoxyribonucleotides, derivatives of ribonucleotides, or mixtures thereof. The nucleic acid aptamer has a binding affinity for an epitope of a surface being treated with the consumer product composition. | 2019-02-14 |
20190048349 | APTAMERS FOR ORAL CARE APPLICATIONS - An aptamer composition is disclosed which has one or more oligonucleotides that include at least one of deoxyribonucleotides, ribonucleotides, derivatives of deoxyribonucleotides, derivatives of ribonucleotides, or mixtures thereof. The aptamer composition has a binding affinity for a material that is at least one of tooth, enamel, dentin, carbonated calcium-deficient hydroxyapatite, or mixtures thereof. | 2019-02-14 |
20190048350 | OLIGOMERS - Certain disclosed oligomers induce exon skipping during processing of myostatin pre-mRNA. The oligomers may be in a vector or encoded by the vector. The vector is used for inducing exon skipping during processing of myostatin pre-mRNA. A therapeutically effective amount of the oligomer may be administered to a subject patient such that exon skipping during processing of myostatin pre-mRNA is induced. The administration to a subject may be used in order to increase or maintain muscle mass, or slowing degeneration of muscle mass in the subject. The administration to a subject may ameliorate muscle wasting conditions, such as muscular dystrophy. Examples of such muscular dystrophies which may be so treated include Becker's muscular dystrophy, congenital muscular dystrophy, Duchenne muscular dystrophy, distal muscular dystrophy, Emery-Dreifuss muscular dystrophy, facioscapulohumeral muscular dystrophy (FSHD), limb-girdle muscular dystrophy, myotonic muscular dystrophy, and oculopharyngeal muscular dystrophy. | 2019-02-14 |
20190048351 | MODULATORS OF COMPLEMENT FACTOR B - The present embodiments provide methods, compounds, and compositions for treating, preventing, or ameliorating a disease associated with dysregulation of the complement alternative pathway by administering a Complement Factor B (CFB) specific inhibitor to a subject. | 2019-02-14 |
20190048352 | COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF CD274/PD-L1 GENE - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the CD274/PD-L1 gene, and methods of using such dsRNA compositions to inhibit expression of CD274/PD-L1. | 2019-02-14 |
20190048353 | MARINE BACTERIAL GENE LFLIZ AND USE - It relates to a LfliZ gene and its application. The sequence of gene LfliZ is shown in SEQ ID NO.1. This invention also relates to the recombinant protein LfliZ encoded by LfliZ gene and its application in preparation of 2′,3′-cNMPs. The recombinant LfliZ protein encoded by gene LfliZ can bind four kinds of 2′,3′-cNMPs during its expression in | 2019-02-14 |
20190048354 | PATHWAY INTEGRATION AND EXPRESSION IN HOST CELLS - Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto. | 2019-02-14 |
20190048355 | METHOD FOR DIRECT TRANSFORMATION OF EXOGENOUS DNA INTO RESTING SPORES OF ASPERGILLUS NIGER INDEPENDENT OF MEDIATORS - The present invention discloses a method for direct transformation of exogenous DNA into resting spores of | 2019-02-14 |
20190048356 | PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS - The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors. | 2019-02-14 |
20190048357 | COMPOSITIONS AND METHODS FOR MODIFYING GENOMES - Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci. | 2019-02-14 |
20190048358 | Expression of Foreign Sequences in Plants Using Trans-Activation System - A transactivation system and method for producing a foreign polypeptide of interest in cells of a host plant is disclosed. The transactivation system has two components. It has genetically transformed cells of the host plant having integrated in their nuclear genome, an inactive or silenced foreign nucleic acid sequence, which is capable of encoding, upon its activation, the foreign polypeptide of interest; and a recombinant RNA viral vector capable of infecting the cells of the host plant and encoding therein a factor for activating or facilitating the expression of inactive or silenced foreign nucleic acid sequence. | 2019-02-14 |
20190048359 | NOVEL BIOGASOLINE PRODUCTION VIA MACROALGAL DUCKWEEDS BY THE OPERON EXPRESSION METHOD IL-60 - The present disclosure describes methods of viral induced gene expression in aquatic duckweeds such as | 2019-02-14 |
20190048360 | DOUBLE STRAND RNA-MEDIATED RNA INTERFERENCE THROUGH FEEDING DETRIMENTAL TO LARVAL LYMANTRIA DISPAR (GYPSY MOTH) - The present invention relates to the field of double-stranded RNA (dsRNA)-mediated gene silencing in insect species. More particularly, the present invention relates to genetic constructs designed for the expression of dsRNA corresponding to novel target genes in the insect pest | 2019-02-14 |
20190048361 | EXPRESSION METHOD OF HAEMOCOAGULASE ACUTUS (HALASE) RECOMBINANT PROTEIN - The disclosure relates to an expression method of a Haemocoagulase Acutus (Halase) recombinant protein. The method includes the following steps: (1) optimizing a halase gene; (2) performing Polymerase Chain Reaction (PCR) amplification on an optimized halase gene; (3) constructing an Agkis-pMCX expression vector, transforming plasmids to competent cells of an | 2019-02-14 |
20190048362 | PRODUCTION OF OVERSIZED ADENO-ASSOCIATED VECTORS - Provided herein are methods for producing an adeno-associated virus (AAV) particle containing an oversized recombinant AAV genome (e.g., greater than 4.7 kb). In some aspects, the invention provides AAV particles and AAV vectors comprising oversized rAAV genomes. Producer cell lines to produce AAV particles comprising oversized genomes are also provided. | 2019-02-14 |
20190048363 | AAV-IDUA Vector for Treatment of MPS I-Associated Blindness - This invention relates to viral vectors for delivery of alpha-L-iduronidase to the cornea of a subject and methods of using the same for treatment and prevention of corneal clouding and blindness in a subject due to mucopolysaccharidosis I. | 2019-02-14 |
20190048364 | Methods, tip assemblies and kits for introducing material into cells - Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition. | 2019-02-14 |
20190048365 | MITOCHONDRIAL MICROINJECTION OF OOCYTES - The invention relates to processes for mitochondrial microinjection in oocytes. The processes involve isolating mammalian mitochondria for microinjection in oocytes to increase their mitochondrial activity. Microinjected mitochondria may be isolated from mammalian platelets and incubated in a favorable medium prior to microinjection. Oocytes that are microinjected with mitochondria obtained from the processes of the invention are shown to have a higher rate of fertilization and blastocyst formation when the processes disclosed herein are used concurrently with in vitro fertilization procedures. The invention relates generally to a process for treating deficiencies in mitochondrial activity in oocytes, a process for isolating mitochondria from mammalian platelets, and/or a process for preparing mitochondria for microinjection in oocytes. | 2019-02-14 |
20190048366 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF BUTADIENE - The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene. | 2019-02-14 |
20190048367 | BIOGAS PRODUCTION FROM EXCREMENT - A method for producing biogas includes: anaerobically digesting excrement with an additive, the additive including a pre-pupal stage of an insect from the scientific classification superfamily of Stratiomyoidea; and collecting the biogas. A method for producing an additive for anaerobic digestion of excrement includes: hatching eggs of an insect from the scientific classification superfamily of Stratiomyoidea in excrement; growing insect larvae by feeding with additional excrement; harvesting pre-pupal insect larvae; and grinding the pre-pupal larvae. An apparatus for producing an additive for anaerobic digestion of excrement includes: a tray having pivot along one lateral side and an upwardly sloping wall terminating with a lip along a lateral side opposite the pivot; a flume adjacent the lip; and a conveyor adjacent the pivot. | 2019-02-14 |
20190048368 | A BACTERIAL CELL FACTORY FOR EFFICIENT PRODUCTION OF ETHANOL FROM WHEY - The invention relates to a method for homo-ethanol production from lactose using a genetically modified lactic acid bacterium of the invention, where the cells are provided with a substrate comprising dairy waste supplemented with an amino nitrogen source (such as acid hydrolysed corn steep liquor). The invention further relates to genetically modified lactic acid bacterium and its use for homo-ethanol production from lactose in dairy waste. The lactic acid bacterium comprises both genes (lacABCD, LacEF, lacG) encoding enzymes catalysing the lactose catabolism pathway; and transgenes (pdc and adhB) encoding enzymes catalysing the conversion of pyruvate to ethanol. Additionally a number of genes (ldh, pta and adhE) are deleted in order to maximise homo-ethanol production as compared to production of lactate, acetoin and acetate production. | 2019-02-14 |
20190048369 | DEVICE AND METHOD OF PRODUCING ULTRA-LOW SULFUR BIODIESEL - Provided is a device for producing ultra-low sulfur biodiesel. The device for producing ultra-low sulfur biodiesel is a two-stage processing device, comprising a two-stage purification unit, a two-stage enzyme reaction unit, a two-stage distillation unit, and a decompression rectification unit. The present invention is green and environment-friendly, effectively and completely removing sulfur-containing impurities from the raw material, eliminating the attack of a sulfur-containing group in the synthesis process on fatty acids, and providing sufficient conditions for obtaining ultra-low sulfur content methyl esters in the product section. | 2019-02-14 |
20190048370 | METHOD FOR PRODUCING MULTIPLE OIL/FAT COMPOSITIONS BY COMPLEX TRANSESTERIFICATION REACTION SYSTEM - The present invention addresses the problem of providing a method for effectively producing a target oil/fat that is rich in triglyceride, by using liberated by-products such as fatty acid ester originating from starting material oil/fat that is separated from the target oil/fat efficiently after the reaction when producing the target oil/fat that is rich in triglyceride by a transesterification reaction using, for example, oil/fat and fatty acid ester as starting materials. | 2019-02-14 |
20190048371 | ENZYMATIC ENRICHMENT OF n-3 FATTY ACIDS IN THE FORM OF GLYCERIDES - Disclosed are various enzymatic processes for the enrichments of oils with omega-3 fatty acids, and specific lipase preparations for use with these processes. | 2019-02-14 |
20190048372 | PROCESS FOR THE PREPARATION OF (S)-2-AMINO-NON-8-ENOIC ACID - Disclosed herein is a process for preparing enantioenriched (S)-2-aminonon-8-enoic acid by amination of 2-oxonon-8-enoic acid in the presence of an enzyme and an ammonia source. | 2019-02-14 |
20190048373 | Glutamine Synthetase Reaction and Method for Quantifying Ammonia Utilizing the Same - A reagent for glutamine synthetase reaction comprising a chelating agent and glutamine synthetase, and a reagent for quantification of ammonia comprising a chelating agent, ATP, glutamic acid, glutamine synthetase, glucose, an oxidized NAD compound, ADP-dependent hexokinase, and glucose-6-phosphate dehydrogenase, are provided. | 2019-02-14 |
20190048374 | BIOSYNTHESIS OF ERIODICTYOL FROM ENGINEERED MICROBES - The present invention relates to the production of eriodictyol via bioconversion. | 2019-02-14 |
20190048375 | PROCESS - The present invention relates to processes to make neosaxitoxin, and analogues and variants thereof, and intermediates in the production of neosaxitoxin in recombinant host cells. Neosaxitoxin and the analogues and variants thereof may be used in the production of pharmaceutical compositions. | 2019-02-14 |
20190048376 | ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES - A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example. | 2019-02-14 |
20190048377 | METHOD FOR PRODUCING SACCHARIFIED SOLUTION BY ENZYMATIC METHOD USING CELLULOSE-TYPE BIOMASS AS RAW MATERIAL - A method for producing a saccharified solution making it possible that while the concentration of a solid in a reactor is kept high at an initial stage of hydrolyzing a cellulose contained in a biomass with an enzyme, the biomass is earlier solubilized to be made into a slurry form. In the method, at an initial stage of mixing an aqueous solution containing a biomass pulverized in a reactor, the aqueous solution is poured into reactor, and then pulverized biomass is supplied thereinto step by step while content in reactor is stirred. A final solid concentration in reactor is set into the range of 15 to 30% both inclusive by mass. The reactor's bottom plane is made into a conical or mirror plate form. At least in upper and lower parts of the reactor's inside, plural stirring fans having a long rotation radius are located to stir content in reactor. | 2019-02-14 |
20190048378 | HEMICELLULASE ENRICHED COMPOSITIONS FOR ENHANCING HYDROLYSIS OF BIOMASS - Described are compositions and methods relating to cellulase/hemicellulase enzyme blends for improving the enzymatic hydrolysis of cellulosic and hemicellulosic materials, as commonly found in biomass. | 2019-02-14 |
20190048379 | CELL-FREE PROTEIN EXPRESSION USING DOUBLE-STRANDED CONCATAMERIC DNA - Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/μL to about 35 ng/μL. A RCA product DNA may be used as the double stranded concatemer DNA for the methods. | 2019-02-14 |
20190048380 | INCREASED PRODUCTION OF GINSENOSIDES THROUGH IMPROVEMENT OF PROTEIN-FOLDING MACHINERY OF YEAST - The present invention relates to recombinant yeast, in which the productivity of ginsenoside is enhanced by overexpressing CPR5, PDI1, or ERO1 in yeast having the productivity of ginsenosides; a method for preparing the yeast; and a method for producing ginsenosides using the yeast. | 2019-02-14 |
20190048381 | METHODS OF PRODUCING LIPIDS - Described herein are microoganisms and methods for producing lipids by co-culturing a photosynthetic microorganism with a heterotrophic microorganism to produce a culture medium having a titer of lipids. | 2019-02-14 |
20190048382 | BIOSENSOR FOR MULTI-ANALYTE CHARACTERIZATION - Embodiments of the present invention are directed to a semiconductor device. A non-limiting example of the semiconductor device includes a semiconductor substrate. The semiconductor device also includes a plurality of metal nanopillars formed on the substrate. The semiconductor device also includes an amperometric sensor associated with one of the plurality of nanopillars, wherein the amperometric sensor is selective to an enzyme-active neurotransmitter. The semiconductor device also includes a resistivity sensor associated with a pair of nanopillars, wherein the resistivity sensor is selective to an analyte. | 2019-02-14 |
20190048383 | COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME - The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. | 2019-02-14 |
20190048384 | COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME - The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. | 2019-02-14 |
20190048385 | COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME - The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. | 2019-02-14 |
20190048386 | COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME - The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. | 2019-02-14 |
20190048387 | METHOD AND KIT FOR ASSESSING POSSIBILITY OF CANCERIZATION - A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends. | 2019-02-14 |
20190048388 | METHOD FOR DETERMINING WHETHER OR NOT TEST SAMPLE CONTAINS PHYTOPATHOGENIC FUNGUS - The present invention provides a method for determining whether or not a test sample contains a phytopathogenic fungielectively from two kinds of fungi of a phytopathogenic fungus and a non-phytopathogenic fungus. The method according to the present invention comprises: (a) putting the test sample on a front surface of a substrate comprising a through hole; wherein the substrate comprises a cellulose film on the back surface thereof; the cellulose film has a thickness of not less than 0.5 micrometers and not more than 2 micrometers; and the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; (b) leaving the test sample at rest; (c) observing a back surface of the film; and (d) determining that the test sample contains the phytopathogenic fungus, if a fungus is found on the back surface of the film. | 2019-02-14 |
20190048389 | CULTURE MEDIUM WITH TWO LAYERS FOR DETECTING MICROORGANISMS - To provide a method in which staining of a culture medium itself can be suppressed even if an enzyme that can decompose an enzyme substrate to be decomposed by an enzyme of microorganisms inherently exists in an analyte, and target microorangisms can be detected as stained colony with high accuracy, and an enzyme substrate culture medium therefor. A culture medium for detecting microorganisms includes a first layer in which a gelling agent and an enzyme substrate are contained, and a second layer in which a gelling agent is contained and the enzyme substrate is not contained to be laminated adjacently to the first layer, wherein the enzyme substrate is a compound from which a dye compound can be released. | 2019-02-14 |
20190048390 | CELL CULTURING STRUCTURE INCLUDING GROWTH MEDIUM AND NON-GROWTH MEDIUM - A structure for culturing cells includes growth medium regions on a surface of the structure. Each of the growth medium regions includes a growth medium surface configured to receive and promote growth in a cell that is being cultured. The structure includes a non-growth medium. The non-growth medium includes a non-growth medium surface configured to receive the cell that is being cultured. | 2019-02-14 |
20190048391 | COMPOSITIONS AND METHODS FOR IDENTIFYING ENZYME MODULATORS OR INHIBITORS - The invention is directed to compositions to screen for compounds, e.g., small molecules or drugs, that can modulate or inhibit enzymes, e.g., proteases, such as viral proteases, e.g., HIV proteases; and methods for making and using these compositions. In alternative embodiment, the invention provides compositions and methods for identifying compositions, e.g., drug molecules that can modulate or inhibit enzymes, e.g., proteases, proteinases or peptidases or the like, e.g., HIV proteases. In alternative embodiments, the invention provides cell-based assays to screen for compositions. e.g., small molecules or drugs, that modulate or inhibit or modify the activity of enzymes such as proteases, proteinases or peptidases or the like, such as calcium-dependent protein convertases involved in HIV envelop protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In alternative embodiment, the compositions and methods of the invention are adapted for high through-put or multiplexed screening of compounds, e.g., drug molecules that can modulate or inhibit enzymes. | 2019-02-14 |
20190048392 | APPARATUS AND METHOD FOR CONVERTING ELECTROMAGNETIC RADIATION INTO THERMAL ENERGY - Apparatus for converting electromagnetic radiation into thermal energy for use in isolating nucleic acids for subsequent analysis by a DNA polymerase chain reaction, comprising an electromagnetic radiation generator, which emits microwaves; a chamber, which is connected to the generator and confines the emitted microwaves; a plurality of stationary spots, which are fixedly attached onto the upper part of the chamber and project inwards, wherein the stationary spots are longitudinally separated from each other by identical intervals of predetermined distance, and the emitted electromagnetic radiation propagates within the chamber as a standing wave, and wherein the said predetermined distance is half the wavelength of the emitted radiation. | 2019-02-14 |
20190048393 | METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF MICROORGANISM IN HUMAN BODY - The present invention discloses a method for qualitative and quantitative detection of a microorganism in a human body, which belongs to the field of biotechnology. The method includes the following steps: determining a target microbial population, a target microorganism and a non-target organism in a sample to be tested, as well as a reference microorganism not present in the sample to be tested; designing the characteristic regions of the target microbial population and the target microorganism; designing multiplex amplification primers for the characteristic regions; adding the reference microorganism and an exogenous nucleic acid into the sample to be tested, and then extracting the nucleic acid of the microorganism in the sample to be tested; amplifying the nucleic acid of the microorganism with the designed multiplex amplification primers so as to obtain a characteristic sequencing fragment; and then performing, using the characteristic sequencing fragment, qualitative and quantitative analysis for the microorganism in the sample to be tested. The present invention does not need pre-culture and proliferation of the microorganism, and can perform high throughput, high accuracy and high resolution detection on a plurality of known microorganisms in the sample to be tested at one time, and the detection process is simple, quick and the process is standardized. | 2019-02-14 |
20190048394 | Solid Support for Sample Collection - The present invention relates to means for collection of biological samples. Specifically, the present invention provides a solid support for collection, storage and subsequent analysis of a biological sample as well as methods for use of the solid support of the invention and a kit comprising the solid support of the invention. | 2019-02-14 |
20190048395 | POLYMERASE CHAIN REACTION PATCH, METHOD AND DEVICE FOR DIAGNOSIS USING THE SAME - According to an aspect of the present disclosure, there is provided a polymerase chain reaction (PCR) patch which is provided as a gel type having a net-like structure forming micro-cavities, wherein at least a part of a plurality of reagents used in a PCR are contained in the micro-cavities, and when the patch contacts with an external region, the reagents contained in the micro-cavities move to at least a portion of the external region, and a PCR of a target DNA included in a sample located in the external region is performed. | 2019-02-14 |
20190048396 | LEUKEMIA DIAGNOSTIC KIT TARGETING PROHIBITIN GENE AND DIAGNOSTIC METHOD USING SAME - A leukemia diagnostic kit may be an RT-PCR kit enabling expression levels of prohibitin-1 and prohibitin-2 to be checked in a leukemia patient specimen, wherein the accuracy and the reproducibility of leukemia diagnosis of the kit are higher than those of a conventional RT-PCR kit, thereby being useful as a kit for diagnosing leukemia, examining residual lesions and evaluating therapeutic effects. | 2019-02-14 |
20190048397 | HEATING MECHANISM FOR DNA AMPLIFICATION, EXTRACTION OR STERILIZATION USING PHOTO-THERMAL NANOPARTICLES - A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented. | 2019-02-14 |
20190048398 | ULTRASENSITIVE BIOSENSORS - The present invention is a biosensor apparatus that includes a substrate, a source on one side of the substrate, a drain spaced from the source, a conducting channel between the source and the drain, an insulator region, and receptors on a gate region for receiving target material. The receptors are contacted for changing current flow between the source and the drain. The source and the drain are relatively wide compared to length between the source and the drain through the conducting channel. | 2019-02-14 |
20190048399 | MODIFICATION OF DNA ON MAGNETIC BEADS - Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads. | 2019-02-14 |
20190048400 | COMPOSITIONS AND METHODS FOR THE DETECTION AND ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS - Provided herein are compositions and methods useful for the detection of MTB. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect MTB in samples. | 2019-02-14 |
20190048401 | OPTIMIZED REAL-TIME NUCLEIC ACID DETECTION PROCESSES - This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided. Paneling and multiplex analyses of more than one nucleic acid analyte using one sample are also provided. | 2019-02-14 |
20190048402 | MAGNETIC BEADS-BASED ELECTROCHEMICAL BIOSENSOR - The present invention is generally directed to methods for detecting and/or quantifying miRNA, RNA or DNA molecules of interest in at least one isolated biological sample. Basically the general procedure followed by the methods of the present invention involve four main steps: (i) homogeneous hybridization of the synthetic DNA or RNA probe and the target miRNA, RNA or DNA molecule of interest; (ii) capture of the resultant DNA/RNA, DNA/DNA or RNA/RNA duplex by the antibody-modified magnetic particles, preferably MBs, in solution; (iii) enzymatic labeling of the biotinylated DNA/RNA, DNA/DNA or RNA/RNA duplex captured onto the antibody-modified magnetic particles, preferably MBs and (iv) electrochemical detection of the modified-magnetic particles on an electrochemical sensor, preferably on screen-printed carbon electrodes (SPCEs). | 2019-02-14 |
20190048403 | EXCHANGE-INDUCED REMNANT MAGNETIZATION FOR LABEL-FREE DETECTION OF DNA, MICRO-RNA, AND DNA/RNA-BINDING BIOMARKERS - A method of using an exchange-induced remnant magnetization (EXIRM) technique for label free detection of short strands of nucleotides and cancer biomarkers, such as DNA and microRNA strands, DNA/RNA-binding biomarkers, and cancer-specific antigens, with high sensitivity, high specificity, and broad dynamic range. The method may provide a label-free approach aimed to facilitate high reliability, and to require a minimum amount of biochemical reagents. | 2019-02-14 |
20190048404 | NUCLEIC ACID SEQUENCING METHOD AND SYSTEM EMPLOYING ENHANCED DETECTION OF NUCLEOTIDE-SPECIFIC TERNARY COMPLEX FORMATION - Provided are methods and systems for detecting formation of nucleotide- specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. This advantageously improves signal-to-noise ratios and increases the quality of results obtainable in a sequencing-by-binding protocol, and enables extended read lengths. These results can even be achieved in procedures employing unlabeled, native nucleotides. | 2019-02-14 |
20190048405 | SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS - The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation. | 2019-02-14 |
20190048406 | Oxidising Agent for Modified Nucleotides - This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. A first portion of the population is oxidised with a metal (VI) oxo complex and then the first portion and a second portion of said population are both treated with bisulfite. The residues in the first and second portions that correspond to a cytosine residue in the sample nucleotide sequence are identified following treatment and the identities of these residues are used to determine the modification of the cytosine residue in the sample nucleotide sequence. Methods, reagents and kits are provided. | 2019-02-14 |