17th week of 2014 patent applcation highlights part 41 |
Patent application number | Title | Published |
20140113286 | Epigenomic Markers of Cancer Metastasis - Methods and kits for assessing risk of metastasis in a cancer patient identified as having breast cancer, colon cancer or glioma use analysis of a classifier for CpG island methylator phenotype. | 2014-04-24 |
20140113287 | BASE SEQUENCE ANALYSIS METHOD, BASE SEQUENCE ANALYSIS APPARATUS, AND BASE SEQUENCE ANALYSIS PROGRAM - There is provided a base sequence analysis method including a nucleic acid amplification procedure of obtaining an amplification product by a nucleic acid amplification reaction, a turbidity measurement procedure of measuring turbidity of a reaction solution of the nucleic acid amplification reaction, and a melting curve analysis procedure of performing melting curve analysis of a probe nucleic acid chain and the amplification product at a reaction site of the nucleic acid amplification reaction. | 2014-04-24 |
20140113288 | METHODS OF IDENTIFYING RISK OF PREECLAMPSIA AND PREGNANCY-RELATED DISORDERS - Provided herein are methods of diagnosing a pregnancy disorder (e.g., preeclampsia or related pregnancy disorders) in a subject based on the level of High Temperature Requirement A4 (HtrA4). Also provided herein are diagnostic agents and devices for use in such diagnostic methods. | 2014-04-24 |
20140113289 | COMPOSITIONS AND METHODS TO DETECT LEGIONELLA PNEUMOPHILA NUCLEIC ACID - Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, and detection probes that hybridize specifically to | 2014-04-24 |
20140113290 | ABERRANT METHYLATION OF C6ORF150 DNA SEQUENCES IN HUMAN COLORECTAL CANCER - This application describes methods and compositions for detecting and treating C6Orf150-associated neoplasia. Differential methylation of the C6Orf150 nucleotide sequences has been observed in C6Orf150-associated neoplasia such as colon neoplasia. | 2014-04-24 |
20140113291 | Salt-tolerant DNA polymerases - Four novel sequences of type B DNA polymerases and variants and analogues thereof useful for applications involving DNA polymerization in high salt conditions. | 2014-04-24 |
20140113292 | DETECTING NUCLEIC ACID - This document provides methods and materials for detecting target nucleic acid. For example, methods and materials for detecting the presence or absence of target nucleic acid, methods and materials for detecting the amount of target nucleic acid present within a sample, kits for detecting the presence or absence of target nucleic acid, kits for detecting the amount of target nucleic acid present within a sample, and methods for making such kits are provided. | 2014-04-24 |
20140113293 | METHOD FOR THE DETECTION OF EGFR MUTATIONS IN BLOOD SAMPLES - The present invention refers to the detection of EGFR mutations in a blood (serum/plasma) sample from a subject. The method comprises:
| 2014-04-24 |
20140113294 | DIRECT NUCLEIC ACID AMPLIFICATION KIT, REAGENT AND METHOD - The present invention relates to compositions, methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. The dried reagent composition of the invention can be used for easy processing and amplification of nucleic acid samples. | 2014-04-24 |
20140113295 | APPARATUS FOR ISOLATION OF EXTRACELLULAR VESICLES COMPRISING POROUS POLYMER MONOLITH MICROFILTER - The present invention relates to an apparatus for the isolation of extracellular vesicles from human fluid, and more particularly, to an apparatus comprising a channel formed on a microchip and a porous polymer monolith filter connected to the channel. The apparatus can be used to the diagnosis of disease including cancer from vesicular nucleic acid in a non-invasive manner. Capable of isolating and purifying a large quantity of vesicles from a small amount of a body fluid sample within a short time, the apparatus is expected to be advantageously and widely applied in the medical research and clinical diagnosis of disease including cancer. | 2014-04-24 |
20140113296 | ADDITION OF AN ADAPTOR BY INVASIVE CLEAVAGE - This disclosure provides method for adding an adaptor to a genomic sequence by invasive cleavage, as well as a kit for performing the method. In some embodiments, the method comprises: a) hybridizing genomic DNA to an adaptor comprising a double stranded region and a single stranded region comprising a 5′ overhang to produce a substrate for a flap endonuclease; b) cleaving the substrate using the flap endonuclease; c) ligating the recessed end of the double stranded region to the fragment to produce an adaptor-ligated DNA; d) intramolecularly ligating the ends of the adaptor-ligated DNA to produce a circular DNA molecule; and e) enzymatically processing the circular DNA molecule using an oligonucleotide that hybridizes to the adaptor and an enzyme. A kit for performing the method is also provided. | 2014-04-24 |
20140113297 | GENE EXPRESSION PREDICTORS OF CANCER PROGNOSIS - Disclosed herein are methods of predicting the prognosis of a subject with prostate cancer. The methods include determining the expression level of a gene product of one or more of ZWILCH, DEPDC1, TPX2, CDCA3, HMGB2, MYC, CDC20, and/or KIF11. Expression of the gene product above a threshold level of expression indicates a poor prognosis such as a likelihood of relapse. | 2014-04-24 |
20140113298 | DETECTION AND MIXING IN A CONDUIT IN INTEGRATED BIOANALYSIS SYSTEMS - Apparatuses and methods in which detection is integrated with various liquid processing and environmental control functions to create integrated bioanalysis systems are disclosed. Though the various integrated bioanalysis systems are useful for any number of analysis formats, they are adaptable to high-throughput processing of samples. | 2014-04-24 |
20140113299 | INHIBITION-RESISTANT POLYMERASES - Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such. | 2014-04-24 |
20140113300 | METHODS FOR ANALYZING DNA - The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample. | 2014-04-24 |
20140113301 | DETECTION OF SAXITOXIN-PRODUCING DINOFLAGELLATES - The current disclosure generally relates to the field of saxitoxins and the identification of microorganisms capable of producing them. In particular, the saxitoxin A (sxtA) gene comprising catalytic domain sequences saxitoxin A1 (sxtA1) and saxitoxin A4 (sxtA4) is identified in a number of dinoflagellate species. The disclosure relates to methods of detection of saxitoxin-producing dinoflagellates by amplification and detection of the sxtA gene (in particular by PCR) and kits and primers for use in the method are also disclosed. Saxitoxin-producing dinoflagellate genera detected by the method include | 2014-04-24 |
20140113302 | METHODS FOR SEQUENCING, AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS COMPRISING INTERNALLY LABELLED PRIMER - The present application provides for an enhanced method for sequencing, or amplifying and detecting nucleic acids comprising the steps of: i) providing at least one nucleic acid primer comprising at least one labelled nucleotide, ii) providing enzymes and reagents for amplification of a target nucleic acid using said at least one labelled nucleic acid primer, iii) incubating the components of the reaction under conditions suited for amplification of the target nucleic acid primer, iv) detecting the amplified nucleic acids via the labelled nucleotide, wherein the nucleic acid primer comprises the following sequence: 5′-N | 2014-04-24 |
20140113303 | Chemical Coating of Microwell for Electrochemical Detection Device - The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical. | 2014-04-24 |
20140113304 | Bi-Directional Cytosine Deaminase-Encoding Selection Marker - The present invention relates to a bi-directional cytosine deaminase-encoding selection marker as well as methods for using said marker. | 2014-04-24 |
20140113305 | EXOSOMES AND DIAGNOSTIC BIOMARKERS - The invention provides methods for the detection of | 2014-04-24 |
20140113306 | DIAGNOSTIC AND SCREENING METHODS FOR INFLAMMATION - This invention is directed to methods, kits and compositions for the diagnosis and treatment of conditions associated with sub-clinical inflammatory conditions such as diabetes mellitus. | 2014-04-24 |
20140113307 | METHOD FOR ANALYZING PROTEIN-PROTEIN INTERACTION ON SINGLE-MOLECULE LEVEL IN CELL ENVIRONMENT, AND METHOD FOR MEASURING DENSITY OF PROTEIN ACTIVATED IN CYTOSOL - A method of analyzing protein-protein interactions includes: preparing two substrates, in which first protein-binding molecules that are biomolecules to be bound to the first proteins are attached to each of the substrates; inducing binding between the first proteins and the first protein-binding molecules on the first substrate and the second substrate, respectively, by supplying the first proteins included in the control group-cell to the first substrate among the two substrates and supplying the first proteins included in the experimental group-cell to the second substrate among the two substrates; supplying cell lysates of cells including the marker-tagged second proteins to the first substrate and the second substrate, respectively; and comparing the interactions between the first proteins and the second proteins on the first substrate and the second substrate in the supply of the cell lysates to the first substrate and the second substrate, respectively. | 2014-04-24 |
20140113308 | MCAM AS A BIOMARKER FOR FLUID HOMEOSTASIS - The application discloses MCAM as a new biomarker for fluid homeostatic imbalance; methods for predicting, diagnosing, prognosticating and/or monitoring fluid homeostatic imbalance based on measuring said biomarker; and kits and devices for measuring said biomarker and/or performing said methods. | 2014-04-24 |
20140113309 | METHOD AND APPARATUS FOR ANALYZING PROTEIN-PROTEIN INTERACTION ON SINGLE-MOLECULE LEVEL WITHIN THE CELLULAR ENVIRONMENT - A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values. | 2014-04-24 |
20140113310 | CANCER DETECTION MARKERS - Methods and compositions involving molecular markers for the detection and characterization of cancer in a patient are provided. | 2014-04-24 |
20140113311 | AGGLUTINATION ENHANCER - The purpose of the present invention is to provide an agglutination enhancer which shows superior agglutination enhancing effect to those of conventional immunoagglutination enhancers, and the present invention relates to an agglutination enhancer for an immunoagglutination measurement method which comprises a polymer having a monomer unit shown by the following general formula [1]: | 2014-04-24 |
20140113312 | CLASSIFICATION OF KINASE INHIBITORS USING NONLINEAR OPTICAL TECHNIQUES - A method is disclosed for classifying and distinguishing between type I and type II kinase inhibitors. The method involves the use of non-linear optical techniques, in particular second-harmonic generation (SHG) to identify conformational changes in kinase proteins obtained from known type I or type II inhibitors. The method further involves deducing the manner of binding of unknown inhibitors by comparison with the signal changes produced by known ligands. The method is also applied to comparing the conformational changes induced by the binding of generic and branded kinase inhibitor drugs to a target kinase. | 2014-04-24 |
20140113313 | METHOD OF DIAGNOSING ADOLESCENT IDIOPATHIC SCOLIOSIS - A method for diagnosing an increased risk for developing adolescent idiopathic scoliosis (AIS) in a human subject, comprising detecting the presence or absence of at least one impairment in melatonin-signaling pathway in a cell sample of the subject in the presence and in the absence of a known melatonin-signaling pathway agonist, wherein the cell sample is selected from the group consisting of blood cell sample, osteoblast cell sample, osteoclast cell sample and myoblast cell sample, and wherein the presence of the at least one impairment in the melatonin-signaling pathway indicates that the subject possesses an increased risk for developing AIS. | 2014-04-24 |
20140113314 | Two Pore Channels as a Therapeutic Target to Protect Against Myocardial Ischemia and as an Adjuvant in Cardiac Surgery - The present invention relates to methods and compositions for modulating the activity of two-pore domain K+ channels (“K | 2014-04-24 |
20140113315 | Cell separation method using a release system for cell-antibody-substrate conjugates containing a polyethylene glycol spacer unit - The invention is directed to a releasable conjugate comprising a biotinylated ligand having a biotin moiety, a ligand moiety (Ligand | 2014-04-24 |
20140113316 | DEVICE, METHOD AND KIT FOR THE DETECTION OF DIFFERENT MARKERS IN DIFFERENT CELLULAR OR MOLECULAR TYPES AND THEIR QUANTIFICATIONS - Microplates or microstrips having wells with extended length to receive immunosorbent elements protruding from a rod at the same modular distance of wells arranged in standard microstrips or microplates and methods of use are provided. A solid phase is constituted by the extended wells, each of which immobilize a different type of cell or molecule in the sample; the other solid phase is constituted by immunosorbent elements protruding from a rod, each of which has been previously coated with one of the same markers to be detected in the sample. Ligands for markers to be detected are added in a liquid phase to the wells; allowing the ligands competitively bind to the immunosorbent elements, inversely proportional to the quantity of the markers of each type of cell or molecule immobilized on the proper extended well. These ligands are simultaneously quantifiable by an immunoenzymatic assay using chromogenic substrate and a spectrophotometer. | 2014-04-24 |
20140113317 | POLYCLONAL ANTIBODIES TO CLONED FUNGAL POLYPEPTIDE TARGETS - Described herein are processes for preparing a polyclonal antibody which specifically binds to a cDNA derived fungal allergen. Such antibodies, for example, polyclonal antibodies specific for cloned Alt a | 2014-04-24 |
20140113318 | Novel Biomarkers - Provided herein are, inter alia, methods for predicting the susceptibility of a subject to a mental or neurodegenerative disorder, the method comprising obtaining one or more biological samples from the subject; determining the levels of one or more biomarkers in the sample, wherein the biomarkers are selected from pyrroles, histamine, methionine adenosyltransferase (MAT) activity, homocysteine, copper and zinc; and comparing the level(s) of the biomarker(s) determined in (b) with the level(s) of said biomarker(s) from one or more control samples, wherein abnormal levels of the one or more biomarkers in the sample(s) from the subject compared to the one or more control samples is predictive of susceptibility of the subject to a mental or neurodegenerative disorder. | 2014-04-24 |
20140113319 | METHODS FOR DETERMINING THE RISK OF PRENATAL COMPLICATIONS - The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. | 2014-04-24 |
20140113320 | METHODS FOR DETERMINING THE RISK OF PRENATAL COMPLICATIONS - The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. | 2014-04-24 |
20140113321 | IDENTIFICATION OF CELLULOLYTIC MICROORGANISM CONTAMINATION IN FOOD AND OTHER MATERIALS - Compositions, kits, and methods for detecting cellulolytic microorganisms in a sample are disclosed herein. In some embodiments cellulolytic microorganisms are detected by detecting the presence of a secreted enzyme, such as but not limited to cellobiohydrolase. | 2014-04-24 |
20140113322 | SUPRAMOLECULAR NANOBEACON IMAGING AGENTS AS PROTEASE SENSORS - Disclosed herein are novel nanobeacon imaging agents having the following formula: | 2014-04-24 |
20140113323 | DETERMINATION OF PHYSICAL PARAMETERS ASSOCIATED WITH ERYTHROCYTES - A method based on digital holography for determining parameters of at least one erythrocyte is provided, said method comprising the steps of: constructing an interference pattern comprising a wave front representative of said at least one erythrocyte arised from the interaction of said at least one erythrocyte and electromagnetic radiation, reconstructing amplitude- and phase information representative of said at least one erythrocyte wave front from said interference pattern and determining the mean corpuscular volume and/or mean corpuscular hemoglobin concentration and/or oxygen saturation and/or mean corpuscular hemoglobin of at least one erythrocyte by combining phase information and amplitude information representative of said at least one erythrocyte. | 2014-04-24 |
20140113324 | SYSTEM AND METHOD FOR DEFORMING AND ANALYZING PARTICLES - A system for deforming and analyzing particles includes a substrate defining an inlet, and an outlet; a fluidic pathway fluidly coupled to the inlet and the outlet and defining a delivery region upstream of a deformation region configured to deform particles, wherein the fluidic pathway comprises a first branch configured to generate a first flow, and a second branch configured to generate a second flow that opposes the first flow, wherein an intersection of the first flow and the second flow defines the deformation region; a detection module including a sensor configured to generate a morphology dataset characterizing deformation of the particles, and a photodetector configured to generate a fluorescence dataset characterizing fluorescence of the particles; and a processor configured to output an analysis of the plurality of particles based at least in part on the deformation dataset and the fluorescent dataset for the plurality of particles. | 2014-04-24 |
20140113325 | USE OF FLUORESCENT SACCHARIDE-BASED DERIVATIVE - The disclosure provides a use of a fluorescent saccharide-based derivative in cell detection. The fluorescent saccharide-based derivative is obtained from a reactive material through an imine formation reaction, and the reactive material is selected from a composition (A) or a composition (B). The composition (A) includes a reducing sugar compound and an amino group-containing compound having at least one primary amino group. The composition (B) includes an amino sugar compound and a carbonyl group-containing compound, and the amino sugar compound contains at least one primary amino group. | 2014-04-24 |
20140113326 | KIT FOR DETECTING BIOFILMS - The invention relates to a kit for detecting biofilms, which is in particular compatible with the agri-food industry and comprises a biofilm staining solution containing a stain in solution in a dilution phase compatible with the agri-food industry, wherein said stain is Coomassie blue, and a cleaning solution comprising said dilution phase. | 2014-04-24 |
20140113327 | BIOCHEMICAL SENSOR FOR QUANTITATIVE SIMULTANEOUS MULTI-SPECIES BACTERIA DETECTION IN SITU - Methods for detecting concentration target organisms in water. The methods involve adding a tagged reagent to a water sample, wherein the tagged reagent is water soluble; and determining a concentration of at least one bacteria in the water sample based on an intensity of an emission emitted from the water sample in response to exposure to light having a known wavelength. An apparatus including a reaction chamber; a reversible pump, a reagent source comprising a fluorophore-tagged reagent, a light source, an optical detector disposed to detect fluorescence emitted from the reaction chamber in response to light emitted from the light source; a processor configured to communicate with the reversible pump, the reagent source, the light source, and the optical detector, the processor being configured to determine the concentration of one or more target organisms in a water sample. | 2014-04-24 |
20140113328 | BIOPSY SUPPORT WITH SECTIONABLE RESILIENT CELLULAR MATERIAL - A histologic tissue sample support device includes a tissue support formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. A resilient cellular material is coupled to the tissue support and is configured to engage and retain tissue in place during processing and embedding. The resilient cellular material is also capable of successful sectioning in the microtome and porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material used to embed the tissue while the tissue is retained by the resilient cellular material. | 2014-04-24 |
20140113329 | HIGH-CELL DENSITY FED-BATCH FERMENTATION PROCESS FOR PRODUCING RECOMBINANT PROTEIN - Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided. | 2014-04-24 |
20140113330 | MODIFIED VITAMIN K-DEPENDENT POLYPEPTIDES - The invention provides vitamin K-dependent polypeptides with enhanced membrane binding affinity. These polypeptides can be used to modulate clot formation in mammals. Methods of modulating clot formation in mammals are also described. | 2014-04-24 |
20140113331 | Systems and Methods for Cultivating, Harvesting and Processing Biomass - Combining controlled open-ocean iron enrichment with a system for collecting the ensuing biological growth can lead to a fundamental shift towards using marine biomass feedstock for large-scale global biodiesel production. The literature review reveals that open-ocean enrichment effectively reduces both the atmospheric carbon dioxide partial pressure and ocean acidity. A semi-closed ocean system is provided that allows for the efficient cultivation and harvesting of a high tonnage biomass feedstock generated by iron fertilization. The concept methodically capitalizes on the ocean's free nutrients, kinetic/potential energy, and expansive surface area to ensure that the mass, energy, and cost balance equations favor our system while taking care to preserve the ocean's ecosystem. The system is modular, portable, easily scalable system, and minimizes waste. In addition to the above benefits, our concept allows continued adherence to the NEPA and London Protocol by culling the biomass produced by fixing carbon dioxide and limiting iron exposure to the vessel's interior. | 2014-04-24 |
20140113332 | TEMPLATE SWITCH-BASED METHODS FOR PRODUCING A PRODUCT NUCLEIC ACID - Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits. | 2014-04-24 |
20140113333 | Whole Transcriptome Sequencing - Methods for preparing strand-specific sequencing libraries of oligonucleotides using an RNA polymerase promoter to re-transcribe antisense cDNA which has been reverse transcribed from mRNA are provided. The transcription step linearly amplifies sRNA prior to production of double-stranded cDNA to be sequenced and may be sufficient to eliminate the conventional PCR amplification step prior to sequencing. The methods incorporate anchor sequences, amplification sequences and other sequences required for a particular sequencing system or reaction by hybridization and extension of primers, and transcription of RNA, rather than ligation, thus reducing the number of steps and the time required for sample preparation for sequencing of RNA. Use of primer hybridization and transcription reactions in the methods also results in a library that exhibits reduced 3′ sequence bias. | 2014-04-24 |
20140113334 | METHOD AND APPARATUS FOR MIXING A LIGNOCELLULOSIC MATERIAL WITH ENZYMES - A method to treat a lignocellulosic material including: pretreating the lignocellulosic material to produce a slurry; adding an alkali material to the slurry in a first conveyor; adding a first portion of an enzyme solution to the slurry while the slurry is in, entering or passing through the discharge of a second conveyor; transferring the slurry with the enzyme solution from the second conveyor slurry to a mixer; mixing the slurry with a second portion of the enzyme solution, and transferring the slurry with the enzyme solution mixed with the first and second portions of the enzyme solution to a first reactor vessel for enzymatic hydrolysis, wherein the viscosity of the slurry is reduced in the first reactor vessel. | 2014-04-24 |
20140113335 | Improved Cellulase Variants - This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in | 2014-04-24 |
20140113336 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-04-24 |
20140113337 | Process for Stimulating Hyaluronic Acid Production with Ascorbic Acid Derivatives and the Composition Thereof - This invention discloses a process and composition for stimulating the production of hyaluronic acid. The mentioned process comprises reacting fibroblast cell with ascorbic acid derivatives. This invention provides a not only easy and un-expensive but also more efficient method for preparing safe hyaluronic acid by stimulatory in vitro. | 2014-04-24 |
20140113338 | PREPARATION OF 5-FORMYL VALERIC ACID FROM ALPHA-KETOPIMELIC ACID - The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ε-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme. | 2014-04-24 |
20140113339 | METHOD FOR CONVERTING AND PRODUCING CARBONATE MINERALS FROM CARBON DIOXIDE USING RECOMBINANT BIOCATALYST - The present invention relates to a technique for capturing carbon dioxide and converting the carbon dioxide to carbonate minerals using a recombinant whole cell biocatalyst expressing carbonic anhydrase. More particularly, the present invention relates to a composition for capturing carbon dioxide and a method for capturing carbon dioxide using the composition, which composition comprises a whole cell of a transformant formed with a vector including a nucleic acid encoding a recombinant carbonic anhydrase; a cell lysate or its fraction of the whole cell; or a recombinant carbonic anhydrase isolated from the whole cell. Further, the present invention relates to a composition and method for converting the carbon dioxide to carbonate minerals using the carbon dioxide capturing composition. | 2014-04-24 |
20140113340 | HIGH SALINITY TOLERANT MICROALGAE STRAINS, PRODUCTS DERIVED THEREFROM, AND METHODS OF PRODUCING THE SAME - The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products. | 2014-04-24 |
20140113341 | ABE FERMENTATION METHOD COMPRISING PRODUCT ABSORPTION THROUGH ISOPHORON - The invention relates to a method comprising the following method steps: A) providing an aqueous solution comprising microorganisms producing low-molecular, organic compounds; B) introducing at least one gas or gas mixture into the aqueous solution; C) recovering the gas flow through a compound comprising an isophoron; and optionally D) separating the low-molecular, organic compound from the composition comprising the isophoron. | 2014-04-24 |
20140113342 | Production of 1,2-Propanediol in Cyanobacteria - Cyanobacterial host cells are modified to produce 1,2-propanediol. | 2014-04-24 |
20140113343 | MODIFIED MICROORGANISMS AND USE THEREOF FOR TERPENE PRODUCTION - The present invention relates to the control of gene expression by a heterologous glucose-regulated promoter, to microorganisms in which gene expression is controlled by a heterologous glucose-regulated promoter and to methods using said microorganisms for the production of terpenes during glucose-limited fed-batch fermentation. | 2014-04-24 |
20140113344 | ISOPRENE SYNTHASE AND GENE ENCODING THE SAME, AND METHOD FOR PRODUCING ISOPRENE MONOMER - The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c):
| 2014-04-24 |
20140113345 | Apparatus and Method for Culturing Cells and Tissue - An apparatus that provides synchronized chronic electrical stimulation and chronic stretch to a cell culture or tissue. The apparatus includes a programmable controller which synchronizes stretch and electrical stimulation applied to a deformable culture container, such as a silicone rubber culture dish. The apparatus is used to treat cells and retard de-differentiation or promote differentiation and maturation in some applications. | 2014-04-24 |
20140113346 | THERMOSTABLE CARBONIC ANHYDRASES AND METHODS OF USE THEREOF - The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide. | 2014-04-24 |
20140113347 | Biopolymer Composition for Encapsulating Cells, Method for Producing a Biopolymer Composition for Encapsulating Cells, Method for Promoting Cell Cytoprotection and Use of a Biopolymer Composition for Encapsulating Cells - The present invention relates to a biopolymer composition for encapsulating cells, containing alginate, at least one glycosaminoglycan compound, preferably chondroitin sulfate, and at least one component of the extracellular matrix, preferably laminin, and to the process for producing the biopolymer composition. Also disclosed are a method for promoting cytoprotection using this composition, and the use of this composition for preparing a medicament useful in cell transplantation. | 2014-04-24 |
20140113348 | MEDITOPES AND MEDITOPE-BINDING ANTIBODIES AND USES THEREOF - Antibodies and meditopes that bind to the antibodies are provided, as well as complexes, compositions and combinations containing the meditopes and antibodies, and methods of producing, using, testing, and screening the same, including therapeutic and diagnostic methods and uses. | 2014-04-24 |
20140113349 | Solubilized Phospholipids for Stabilizing Nucleic Acid Polymerases - Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule. | 2014-04-24 |
20140113350 | METHOD FOR KILLING MICROORGANISM - Provided is a technique for enabling a liquid of microbial cells having an enzymatic activity to be easily stored and used. The technique is a method for killing a microorganism while maintaining the enzyme titer of a microbial cell liquid, characterized by including adjusting the pH of a liquid of microbial cells having an enzymatic activity, and then performing a heating treatment of the liquid, and also the technique is a method for killing a microorganism while maintaining the enzyme titer of a microbial cell liquid, characterized by including adding a carbohydrate to a liquid of microbial cells having an enzymatic activity, and then performing a heating treatment of the liquid. | 2014-04-24 |
20140113351 | PREPARATION AND USE OF BIOFILM-DEGRADING, MULTIPLE-SPECIFICITY, HYDROLYTIC ENZYME MIXTURES - The present invention relates to isolated structures containing degradative enzymes produced from a marine organism. The enzymes produced are based on the carbon source upon which the marine organism is growing. The enzymes are found in structures that can be isolated such that the degradative enzymes are easily harvested. | 2014-04-24 |
20140113352 | RECOMBINANT THERMOASCUS AURANTIACUS BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS - The present invention provides compositions and methods for the expression of recombinant β-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass. | 2014-04-24 |
20140113353 | Method to Produce Virus in Cultured Cells - A method for improving virus production in a host cell infected with the virus is provided. | 2014-04-24 |
20140113354 | COMPOSITION CONTAINING MICROBIAL ZEAXANTHIN AND PREPARATION THEREOF - The present invention relates to a composition for prevention and/or treatment of human skin alteration or disease comprising an extract of marine bacteria selected from Flavobacteriaceae, wherein the extract contains an effective amount of zeaxanthin. The present invention also relates to a method for preparing the above composition, comprising: (a) culturing marine bacteria selected from Flavobacteriaceae, more specifically, from | 2014-04-24 |
20140113355 | PROCESS FOR OBTAINING AN IgG COMPOSITION THROUGH HEAT TREATMENT - A process for obtaining an IgG composition involves heat treatment. This process obtains an IgG composition from an IgG solution partly purified from human plasma, in which by applying intermediate heat treatment and without using reagents to precipitate high molecular weight aggregates/polymers and/or proteins virtually total elimination of the IgG polymers generated during the process is achieved. Furthermore this process offers high productivity, lower production costs and is easy to implement in comparison with the processes of the know art. In addition to this, by using this process stability is imparted to the final product in liquid. | 2014-04-24 |
20140113356 | NANO-ELECTRODE BASED CHIP - The present invention disclosed a nano-electrode based transparent chip, which is applicable to perform a micro fluidic substance diffused through a specific region of a single cell membrane by localized electroporation technique with membrane reversibility. The chip comprises a silicon-based layer, different structural layers, an insulating layer and a micro fluidic layer. When the individual single cell was placed on the gap between a plurality of triangular-shaped nano-electrodes with nano-tip, then electric field can intense on a specific region of the individual single cell causes the formation of nano-pores on the membrane, resulting to deliver drugs, DNA molecules from outside of the cell to the inside of the cell with a high transfection rate and a high cell viability. This technique not only generates well-controlled nano-pores to allow rapid recovery of the cell membrane, but also provides clear optical path for potentially monitoring/tracing the drugs into the cell. | 2014-04-24 |
20140113357 | REMOTE CHEMICAL ASSAY SYSTEM - A portable device for remote chemical assay classification, comprising a computer processor, and an apparatus implemented on the computer processor, the apparatus comprising: an out-of-sample data receiver, configured to receive data defining an out-of-sample extension extracted on a remote computer from classifying test assays of a chemical reaction on the remote computer into at least two groups, and an assay classifier, in communication with the out-of-sample data receiver, configured to classify a new assay of the chemical reaction into one of the groups, using the data defining the out-of-sample extension. | 2014-04-24 |
20140113358 | Systems and Methods for Focusing Particles - Provided is a method of focusing particles. The method includes: providing a suspension of the particles in a suspending medium; and flowing the suspension along a channel, such that the flowing suspension occupies a certain volume that has at least one cross-sectional dimension smaller than 100 μm. The suspending medium has such viscoelastic properties, that flowing the suspension in the channel directs at least some of the particles towards a focus region, enclosed in said certain volume. | 2014-04-24 |
20140113359 | HIGH SENSITIVITY SENSOR DEVICE AND MANUFACTURING THEREOF - A sensing device able to do concurrent real time detection of different kinds of chemical, biomolecule agents, or biological cells and their respective concentrations using optical principles. The sensing system can be produced at a low cost (below $1.00) and in a small size (˜1 cm | 2014-04-24 |
20140113360 | CELL CHIP CONTAINER FOR STORING CELL CHIP - There is provided a cell chip including: a first substrate provided with a culture region receiving a bio-material and a humidity control space storing water for maintenance of humidity; a second substrate coupled to the first substrate; and a first wall member surrounding the culture region. | 2014-04-24 |
20140113361 | PLANT FOR PRODUCTION OF BIOGAS - The present disclosure refers to a plant ( | 2014-04-24 |
20140113362 | Closed Algae Cultivation System - Disclosed herein is an improved closed algae cultivation system that has an algae supplier, a first conduit, at least two containers, and at least two light light-emitting devices. The algae supplier is coupled to the at least two containers via the first conduit. Each container includes at least one sidewall, a bottom and an upper lid, which together define an accommodating volume for accommodating an algal stock culture from the algae supplier. Each container is made of at least one opaque material and includes a first opening disposed on one of the at least one sidewall, and a second opening disposed at the bottom. The at least two light-emitting devices are respectively coupled to the upper lid of each container and extend therefrom along the longitudinal direction to the container such that at least a portion of the light light-emitting devices is submerged in the liquid algal stock culture | 2014-04-24 |
20140113363 | PROCESS OF PRODUCING OIL FROM ALGAE USING BIOLOGICAL RUPTURING - A process for production of biofuels from algae can include cultivating an oil-producing algae, extracting the algal oil, and converting the algal oil to form biodiesel. Extracting the algal oil from the oil-producing algae can include biologically rupturing cell wall and oil vesicles of the oil-producing algae using at least one enzyme such as a cellulose or glycoproteinase, a structured enzyme system such as a cellulosome, a virus, or combination of these materials. | 2014-04-24 |
20140113364 | CELL CULTURE DEVICE - There is provided a cell culture device including: a cell chip receiving part receiving a cell chip; a drug storing part storing a drug; and a circulation part connecting the cell chip receiving part and the drug storing part to one another and circulating the drug therebetween. The cell culture device may allow for the observation or examination of a reaction between a cell and a drug in an environment similar to that of the inside of a body. | 2014-04-24 |
20140113365 | CELL CULTURING VESSEL - A cell culture vessel supplies cells close to the center of gel and deeper down to the bottom with sufficient nutrients for attaining an enhanced survival rate of the cultivated cells. The cell culturing vessel comprises a culture tray capable of holding a liquid culture medium and a cell mixture container fixed to inner walls of the culture tray for retaining the cell mixture. Once the culture tray is filled with the liquid culture medium, the cell mixture retained in the cell mixture container has its opposite outer parts exposed to the liquid culture medium. | 2014-04-24 |
20140113366 | MICROFLUIDIC SYSTEM FOR CONTROLLING THE CONCENTRATION OF MOLECULES FOR STIMULATING A TARGET - The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device ( | 2014-04-24 |
20140113367 | Systems, Methods and Compositions for Optical Stimulation of Target Cells - Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments). | 2014-04-24 |
20140113368 | REPLICATION DEFICIENT RECOMBINANT VIRUSES EXPRESSING ANTIGENS REGULATED BY TRANSCRIPTIONAL CONTROL ELEMENTS COMPRISING MULTIPLE ELEMENTS - The present invention relates to a replication deficient recombinant virus encoding at least one antigen and/or antigenic epitope, wherein expression of said antigen and/or antigenic epitope is regulated by a transcriptional control element comprising at least two elements driving early expression of said antigen and/or antigenic epitope and the use of said replication deficient recombinant virus as medicament or vaccine. | 2014-04-24 |
20140113369 | Procurement Of Placental Stem Cells - Methods and solutions for the preservation and procurement of placental stem cells. A method for collecting stem cells from a placenta includes draining cord blood from the placenta and collecting the drained cord blood in a first collection. The drained placenta is perfused with a first perfusion solution including a placental preservative base solution and a vasodilator. The placenta is perfused with a second perfusion solution including placental preservative base solution, a stem cell releasing agent, an antibiotic, and an anticoagulant, and a predetermined amount of time is allowed to elapse as the first and second perfusion solutions perfuse the placenta. The first and second perfusion solutions, which contain stem cells from the placenta, are then collected in a second collection. | 2014-04-24 |
20140113370 | FC FUSION PROTEINS COMPRISING NOVEL LINKERS OR ARRANGEMENTS - The application provides Fc fusion proteins having novel arrangements. In one embodiment, the application provides Fc fusion proteins comprising a | 2014-04-24 |
20140113371 | Cryopreservation of Haptenized Tumor Cells - A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided. | 2014-04-24 |
20140113372 | CELL CULTURE SUBSTRATE, AND CELL CULTURING METHOD USING THE SUBSTRATE AND METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS USING THE SUBSTRATE - [Problem] To provide a cell culture substrate, and a cell culturing method using the substrate and a method for inducing differentiation of pluripotent stem cells using the substrate, which allow culturing of pluripotent stem cells and allow differentiation of pluripotent stem cells into a specified cell species, particularly neural and neural progenitor cells, at a high purity. | 2014-04-24 |
20140113373 | THREE DIMENSIONAL SOY PROTEIN-CONTAINING SCAFFOLDS AND METHODS FOR THEIR USE AND PRODUCTION - Porous soy protein-based scaffolds and methods for making the scaffolds using 3D printing techniques are provided. Also provided are tissue growth scaffolds comprising the porous soy protein-based scaffolds and methods for growing tissue on the tissue growth scaffolds. The porous soy protein-containing scaffold comprises a plurality of layers configured in a vertical stack, each layer comprising a plurality of strands comprising denatured soy proteins. | 2014-04-24 |
20140113374 | CELL LINES THAT OVEREXPRESS LACTATE DEHYDROGENASE C - Cell lines that overexpress lactate dehydrogenase C (LDH-C) are disclosed. Generally, the cells include a heterologous polynucleotide molecule that encodes a LDH-C polypeptide or a fragment or biologically active analog that possesses measurable LDH-C activity. Also disclosed is a method of increasing the productive yield of a cell that produces a therapeutic agent. Generally, the method includes introducing into the cell a heterologous polynucleotide molecule that encodes a LDH-C polypeptide or a fragment or biologically active analog that possesses measurable LDH-C activity. | 2014-04-24 |
20140113375 | Transient Expression And Reverse Transcription Aided Genome Alteration System - Disclosed are methods and cellular systems for the generation of predetermined or random alterations at specific genomic location. The invention provides a transient expression and reverse transcription system to generate single-stranded DNA sequences homologous to a target genomic sequence, which can be transported to the nucleus to alter the genetic information of the target genomic sequence. Also provided are cellular and molecular components that can be used to increase the efficiency of the targeted genomic modification process. | 2014-04-24 |
20140113376 | COMPOSITIONS AND METHODS FOR DOWNREGULATING PROKARYOTIC GENES - An isolated polynucleotide is disclosed. The polynucleotide comprises a clustered, regularly interspaced short palindromic repeat (CRISPR) array nucleic acid sequence wherein at least one spacer of the CRISPR is sufficiently complementary to a portion of at least one prokaryotic gene so as to down-regulate expression of the prokaryotic gene and further comprises a nucleic acid sequence encoding at least one CRISPR associated (CAS) polypeptide of a repeat associated mysterious protein (RAMP) family. Uses of the polynucleotides and pharmaceutical compositions comprising the polynucleotides are also disclosed. | 2014-04-24 |
20140113377 | GENETIC MANIPULATION AND EXPRESSION SYSTEMS FOR PUCCINIOMYCOTINA AND USTILAGINOMYCOTINA SUBPHYLA - The present invention relates to the application of isolated promoters and synthetic dominant selection constructs and enhancers for gene targeting for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the | 2014-04-24 |
20140113378 | Method of evaluating fatty liver related disease, fatty liver related disease-evaluating apparatus, fatty liver related disease-evaluating method, fatty liver related disease-evaluating program product, fatty liver related disease-evaluating system, information communication terminal apparatus, and method of searching for prophylactic/ameliorating substance for fatty liver related disease - A method of evaluating fatty liver related disease includes (I) an obtaining step of obtaining amino acid concentration data on a concentration value of an amino acid in blood collected from a subject to be evaluated and (II) a concentration value criterion evaluating step of evaluating a state of a fatty liver related disease including at least one of fatty liver, NAFLD, and NASH in the subject, based on the amino acid concentration data of the subject obtained at the obtaining step. | 2014-04-24 |
20140113379 | Apparatus, System and Method for Consumer Detection of Contaminants in Food Stuffs - An apparatus, system and method of detecting contaminants, such as melamine, in at least one ingestible item. The apparatus, system and method may include a disposable notched probe having therein at least one send and one receive fiber optic, or electrical, or heat source, and a reactant associated with said disposable notched probe, wherein a reaction of the reactant with at least a portion of the ingestible item indicates, to the consumer user, a presence of a contaminant. | 2014-04-24 |
20140113380 | FIELD SAMPLING KIT AND METHODS FOR COLLECTING AND DETECTING ALKYL METHYLPHOSPHONIC ACIDS - The invention relates to kits and methods of collecting and detecting alkyl methylphosphonic acids from environmental or biological liquids using a sorbent material. The sorbent material may be transported, desorbed and tested for alkyl methylphosphonic acids. | 2014-04-24 |
20140113381 | METHOD AND APPARATUS FOR MEASURING BROMATE IONS - A method for measuring bromate ions includes: an adding step of adding a fluorescent substance whose fluorescence intensity changes by the coexistence of bromate ions to sample water; a measuring step of measuring the fluorescence intensity of the fluorescent substance; a difference calculating step of calculating a difference between the fluorescence intensity measured and a reference fluorescence intensity of reference sample water that contains no bromate ion; and a concentration calculating step of calculating bromate ion concentration from the calculated fluorescence intensity difference. The measuring step includes a step of measuring the fluorescence intensity at any one of a case where an excitation wavelength is 264 nm and an emission wavelength is 400 nm, a case where the excitation wavelength is 264 nm and the emission wavelength is 480 nm, and a case where the excitation wavelength is 300 nm and the emission wavelength is 400 nm. | 2014-04-24 |
20140113382 | PLASMONIC JUNCTIONS FOR SURFACE-ENHANCED SPECTROSCOPY - The invention relates to the use of a construct for the detection of an analyte using surface enhanced spectroscopic techniques. The construct includes linked surfaces, where the link is formed by a linker providing a fixed inter-surface separation between the linked surfaces, wherein the linker is a linking compound, and at least one of the surfaces is the surface of a nanoparticle. The linking compound is suitable for interacting with the analyte. The linking compound may be a cucurbituril. The surface enhanced spectroscopic technique may be surface enhanced Raman spectroscopy (SERS). | 2014-04-24 |
20140113383 | FULL RESOLUTION COLOR IMAGING OF AN OBJECT - The invention relates generally to both a method and apparatus for the creation of full resolution color digital images of diagnostic cassettes or objects of interest using a gray-scale digital camera or sensor combined with time sequential illumination using additive primary colors followed by post exposure digital processing. Such procedures and equipment is of significant economic value when employed in situations such as diagnostic clinical analyzers where space is limited and image quality requirements are high. | 2014-04-24 |
20140113384 | SENSOR INTEGRATION IN LATERAL FLOW IMMUNOASSAYS AND ITS APPLICATIONS - Lateral flow immunoassay devices for determining the concentration of an analyte in a sample and methods for measuring analyte concentration in sample using such lateral flow immunoassay devices. | 2014-04-24 |
20140113385 | Compositions and Methods for Identifying Single Antigens or Other Molecules in Cell Preparations - Disclosed are compositions and methods for identifying and preferably quantifying single antigens/molecules in tissue sections and other cell preparations. The methods make use of specific antibodies or aptomers linked with beads or other micro-particles using bright field microscopy having the purpose to identify and quantify single antigens or other molecules. | 2014-04-24 |