18th week of 2021 patent applcation highlights part 33 |
Patent application number | Title | Published |
20210130747 | Production Of Perfume-Containing Shaped Bodies - A method for producing dimensionally stable, fragrance-containing shaped bodies based on hydrocolloids. The present invention is also directed to the shaped bodies produced using this method, to the use of these bodies, and to a washing or cleaning agent which contains these bodies. | 2021-05-06 |
20210130748 | Solid Fragrance-Containing Composition - The invention relates to a method, including: a) producing a melt from sodium acetate trihydrate; b) possibly adding further optional contents; c) possibly adding at least one aesthetic element; d) feeding the melt to a drop former having a rotating, perforated outer drum; e) discharging drops of the melt onto a steel strip by way of the drop former, wherein the mixture output from the drop former has a weight ratio of water to sodium acetate of 0.63 or more. The invention further relates to melting bodies producible by this method. | 2021-05-06 |
20210130749 | SEMICONDUCTOR SUBSTRATE CLEANING AGENT - To provide a cleaning agent that can remove impurities such as metal polishing dust adhering to a semiconductor substrate without corroding metal and can prevent re-adhesion of the impurities. | 2021-05-06 |
20210130750 | CLEANING AGENT AND PREPARATION METHOD AND USE THEREOF - Provided are a cleaning agent and a preparation method and the use thereof. The cleaning agent is prepared from the following raw materials comprising the following mass fraction of components: 0.5%-20% of an oxidant containing iodine, 0.5%-20% of an etchant containing boron, 1%-50% of a pyrrolidinone solvent, 1%-20% of a corrosion inhibitor, 0.01%-5% of a metal ion-free surfactant, and water, with the sum of the mass fraction of each component being 100%, the pH of the cleaning agent is 7.5-13.5, and the corrosion inhibitor is one or more of a benzotriazole corrosion inhibitor, a hydrazone corrosion inhibitor, a carbazone corrosion inhibitor and a thiocarbohydrazone corrosion inhibitor. The cleaning agent can efficiently remove nitrides from hard mask residues with little effects on metals and low-K dielectric materials, and has a good selectivity. | 2021-05-06 |
20210130751 | COMPOSITION FOR SURFACE TREATMENT, METHOD FOR PRODUCING THE SAME, AND SURFACE TREATMENT METHOD USING THE SAME - The present invention provides a means by which it is possible to sufficiently suppress an organic residue while favorably decreasing a ceria residue on a polished object to be polished obtained after being polished using a polishing composition containing ceria. | 2021-05-06 |
20210130752 | SUBSTRATES HAVING WATER-SOLUBLE POLYMER ZONES COMPRISING A FRESHENING ACTIVE AND CONSUMER PRODUCTS COMPRISING THE SAME - A substrate and consumer products made therefrom are provided. The substrate includes a surface and a water-soluble polymer zone disposed on and in contact with the surface. The water-soluble polymer zone includes a freshening active, a water-soluble, film-forming polymer, and a surfactant. | 2021-05-06 |
20210130753 | SOLID FREE-FLOWING PARTICULATE LAUNDRY DETERGENT COMPOSITION - A solid free-flowing particulate laundry detergent composition can include a particle, wherein the particle includes a continuous phase and a discontinuous phase, wherein the continuous phase includes fatty material selected from: fatty acid and/or salts thereof; fatty alcohol; and any combination thereof, wherein the discontinuous phase includes: non-ionic and/or anionic emulsifier surfactant; hydrophobized polyethylene glycol polymer; silicone and/or petrolatum; and perfume. | 2021-05-06 |
20210130754 | METHOD FOR TREATING LIGNOCELLULOSIC BIOMASS - The invention concerns a method for treating lignocellulosic biomass, said method comprising the following steps: a, Preparing an impregnation liquor ( | 2021-05-06 |
20210130755 | PLANT EXTRACT-ENRICHED CONSUMABLE ALCOHOL AND METHOD FOR MANUFACTURING THE SAME - A plant extract-enriched consumable alcohol and a method of producing plant extract-enriched consumable alcohol are disclosed. The plant extract-enriched consumable alcohol includes a plant extract, a first diluting agent, and a second diluting agent. The first diluting agent is mixed with the plant extract to form a mixture and has a purity of more than 99 vol %. The second diluting agent is mixed with the mixture to form the plant extract-enriched consumable alcohol. One of the first diluting agent and the second diluting agent is alcoholic and the other one is non-alcoholic, and an alcohol content of the plant extract-enriched consumable alcohol is less than 50 vol %. | 2021-05-06 |
20210130756 | Biogas Plant - The invention relates to a biogas plant ( | 2021-05-06 |
20210130757 | TISSUE FRAGMENT - Provided is a method for producing a tissue-like construct having cultured cells organized in an aligned manner, especially, a cardiac tissue-like construct. The tissue-like construct prepared by this method is also provided. Further provided are a device for evaluating cellular electrophysiological functions and a method for evaluating cellular electrophysiological functions. Furthermore, a sheet-like cell culture scaffold is also provided. | 2021-05-06 |
20210130758 | BIOCOMPATIBLE COMPOSITE ELEMENTS AND METHODS FOR PRODUCING - A biocompatible composite element for a bioreactor is provided that includes an outer frame and an inner component. The outer frame is a polymeric material. The outer frame can be inseparably attached to a wall of the bioreactor. The inner component is a transparent material selected from a group consisting of glass, sapphire, and glass ceramic. The inner component is secured in the outer component in an inseparable hermetically tight manner. The inner component is configured for a spectral process control through the transparent material of the inner component. | 2021-05-06 |
20210130759 | METHOD OF CULTURING EUKARYOTIC CELLS - An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO | 2021-05-06 |
20210130760 | BIOREACTOR AND RELATED METHODS - An apparatus for culturing cells includes a bio reactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed beds arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed. | 2021-05-06 |
20210130761 | FIXED BED BIOREACTOR AND METHODS OF USING THE SAME - A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon. | 2021-05-06 |
20210130762 | CELL SHEET-FORMING MEMBER, METHOD FOR PRODUCING CELL SHEET-FORMING MEMBER, AND METHOD FOR PRODUCING CELL SHEET - A cell sheet-forming member provided with a surface for forming a cell sheet, wherein the surface comprises a plurality of flat parts and a plurality of concavo-convex parts. Each flat part has a shape extending along a first direction and the plurality of the flat parts are aligned along a second direction intersecting with the first direction over the entire surface. Either of convex parts or concave parts have a stepped structure and individual concave and convex parts include a plurality of the stepped structures which fill gaps between flat parts adjacent to each other, wherein the pitch of said stepped structures is from 100 nm to 10 μm inclusive. | 2021-05-06 |
20210130763 | DEVICE AND METHOD FOR STERILE SAMPLE-TAKING - The invention relates to a device for sampling from bioreactors, comprising a rising line ( | 2021-05-06 |
20210130764 | METHOD AND APPARATUS OF QR-CODED, PAPER-BASED, COLORIMETRIC DETECTION OF VOLATILE BYPRODUCT FOR RAPID BACTERIA IDENTIFICATION - A test device for indole concentrations in headspace gasses of bacterial cultures has a porous substrate imprinted with a wax barrier surrounding a test spot impregnated with p-dimethylaminocinnamaldehyde (DMACA). In embodiments, the test spot lies within a printed bar code and is configured to alter a reading of the bar code when the test spot darkens. The test device may optionally include a second test spot impregnated with Bromthymol Blue, Cobinamide, p-dimethylaminobenzaldehyde, or Chromotropic acid. The device is used by inoculating a sample into a culture; incubating the culture; inserting the test device into airspace of the culture, and observing the test spot for a color change indicative of indole presence. | 2021-05-06 |
20210130765 | CELL CULTURE DEVICE, SYSTEM AND METHODS OF USE THEREOF - The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container. Also provided is a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the wall element of the container is composed of a flexible material. | 2021-05-06 |
20210130766 | MICROORGANISM CULTURE SYSTEM - The microorganism culture system includes: flat carriers to which microorganisms are to be attached; a culture solution supply unit that supplies a culture solution to the carriers from above the carriers; and an effluent tank that stores a culture solution containing the microorganisms flowing out of the carriers. The plurality of carriers are arranged so that surfaces of the carriers are directly opposed to each other or obliquely face each other at an angle. Light irradiation units are installed between the carriers and in at least a part of an outside of the carriers in a horizontal direction and an outside thereof in a vertical direction when viewed in an arrangement direction of the carriers. | 2021-05-06 |
20210130767 | Species of Genus Pholiota and Artificial Cultivation Method and Use Thereof - The invention relates to a rare edible fungus new strain and an artificial cultivation method and use thereof, in particular to a new species | 2021-05-06 |
20210130768 | SALT FORMULATIONS FOR THE FERMENTATION OF MARINE MICROORGANISMS - Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B | 2021-05-06 |
20210130769 | Method for Single Cell Encapsulation via Metabolic Glycoengineering and Copper-Free Click Chemistry - A method of single-cell encapsulation of cells using glycoengineering and click-chemistry is provided. Cells are treated with a precursor for metabolic engineering to modify glycans in a cell membrane and form reactive component A-glycans in the cell membrane suitable for a click-chemistry reaction. The treated cells are suspended in a polymer solution which has a reactive component B suitable for the click-chemistry reaction. The reactive component A-glycans react via the click-chemistry with the reaction component B thereby forming single cell polymer encapsulated cells. Applications include optimizing stem cell function, cell to cell crosslinking, formation of networks of cells or organoids, functionalizing the cells with reactive groups or attaching the cells to a substrate or surface. | 2021-05-06 |
20210130770 | NOVEL BIOMATERIAL SUBSTRATES, CELL CULTURE SYSTEMS COMPRISING THE SAME AND USES THEREOF IN CELL SCREENING APPLICATIONS - The invention relates to the fields of biomaterials, tissue engineering and regenerative medicine. More specifically, it relates to biomaterial substrates having precise surface properties and the use thereof to investigate cell-material interactions. Provided is a cell culture system having a biomaterial substrate which has at least a first linear surface gradient oriented orthogonally to a second linear surface gradient, wherein the first gradient and the second gradient are selected from the group consisting of stiffness (S), (aligned) topography (T) and wettability (W). Also provided is a cell screening platform having a combination of at least two, preferably at least three, more preferably four distinct cell culture systems. | 2021-05-06 |
20210130771 | Ultrathin Interfacial Layer on a Hydrogel to Direct its Surface Properties and Cell Adhesion - A method is disclosed for coating and patterning hydrogels in order to modify surface properties. The method exploits the water content of the hydrogel and the hydrophobicity of the reaction solvent to create a thin oxide adhesion layer on the hydrogel surface. This oxide adhesion layer enables rapid transformation of the hydrophilic, cell non-adhesive hydrogel into either a highly hydrophobic or a cell-adhesive hydrogel by reaction with an alkylphosphonic acid or an α,ω-diphosphonoalkane, respectively. Also disclosed are coated, patterned hydrogels and constructs comprising the coated, patterned hydrogels. | 2021-05-06 |
20210130772 | METHOD FOR TREATING AND MODELLING HEARING LOSS - A method for treating hearing loss in a subject in need thereof is provided. The method includes providing a combination of transcription factors to induce generation of a hair cell-like cell, thereby treating the hearing loss in the subject. The generated hair cell-like cells exhibit characteristic of mature functional cells that is useful in cell replacement therapy for autologous transplantation. | 2021-05-06 |
20210130773 | METHOD AND COMPOSITION FOR GENERATING BASAL FOREBRAIN CHOLINERGIC NEURONS (BFCNs) - The invention relates to methods and compositions for developing basal forebrain cholinergic neurons (BFCNs) from stem cells, and in particular, BFCNs having repaired electrophysiological defects relating to one or more mutations in PSEN2, and to the use of such BFCNs in cell-based therapies to treat Alzheimer's disease. | 2021-05-06 |
20210130774 | HUMAN PLURIPOTENT STEM CELL DERIVED NEURODEGENERATIVE DISEASE MODELS ON A MICROFLUIDIC CHIP - Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip. | 2021-05-06 |
20210130775 | CELLS EXPRESSING A CHIMERIC ANTIGEN RECEPTOR OR ENGINEERED TCR AND COMPRISING A NUCLEOTIDE SEQUENCE WHICH IS SELECTIVELY EXPRESSED - The present invention provides a cell which expresses a chimeric antigen receptor (CAR) or an engineered T-cell receptor (TCR), the cell comprising a nucleotide sequence of interest (NOI) which is selectively expressed by the cell depending on: i) the differentiation/exhaustion state of the cell; or ii) the presence of an environmental metabolite in the microenvironment of the cell. | 2021-05-06 |
20210130776 | METHODS AND COMPOSITIONS FOR MODULATING SUPPRESSION OF LYMPHOCYTE ACTIVITY - The subject matter disclosed herein is generally directed to a novel CD8+ T cell subtype associated with suppressive or regulatory T cell functions. Moreover, the subject matter disclosed herein is generally directed to methods and compositions for use of the subtype. Also, disclosed herein are gene signatures and markers associated with the subtype and use of said signatures and markers. Further disclosed are therapeutic methods of using said gene signatures and immune cell subtype. Further disclosed are pharmaceutical compositions comprising populations of CD8+ TILs depleted for a specific subtype. Further disclosed are interactions with other T cell subtypes. | 2021-05-06 |
20210130777 | METHOD FOR PRODUCING GAMMA DELTA T CELLS - The present invention provides a method for producing a γδT cell from an induced pluripotent stem cell, wherein the induced pluripotent stem cell is derived from a cell other than an αβT cell. | 2021-05-06 |
20210130778 | METHOD FOR LARGE-SCALE PRODUCTION OF HUMAN ALLOSPECIFIC INDUCED-REGULATORY T CELLS WITH FUNCTIONAL STABILITY IN THE PRESENCE OF PRO-INFLAMMATORY CYTOKINES WITH THERAPEUTIC POTENTIAL IN TRANSPLANTATION - A methodology to obtain in vitro large numbers of human induced regulatory T cells with specificity to the donor antigen, with a phenotype and stable suppressor function in the presence of pro-inflammatory cytokines, through of co-cultures of monocyte-derived dendritic cells and T cells “ naïve ”, both from genetically unrelated individuals (donor and recipient) is disclosed. The cells obtained with the present method are of CD4, CD25, CTLA-4 and FOXP3+ phenotype and show a specific suppressor function on donor antigen-specific T lymphocytes. These cells maintain their phenotype and stable suppressive function in presence of pro-inflammatory cytokines TNF-α and IL-6. The stability and the number obtained make them candidates as therapeutic tools for transplantation. | 2021-05-06 |
20210130779 | CLOSED PROCESS FOR EXPANSION AND GENE EDITING OF TUMOR INFILTRATING LYMPHOCYTES AND USES OF SAME IN IMMUNOTHERAPY - The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. The methods may comprise gene-editing at least a portion of the TILs to enhance their therapeutic efficacy. Such TILs find use in therapeutic treatment regimens. | 2021-05-06 |
20210130780 | METHODS OF GENERATING ENUCLEATED ERYTHROID CELLS USING MYO-INOSITOL - Provided herein are methods of generating a population of enucleated erythroid cells. | 2021-05-06 |
20210130781 | METHOD AND APPARATUS FOR PRODUCING PLATELET AND METHOD FOR DETERMINING OPERATING CONDITION OF APPARATUS FOR PRODUCING PLATELET - Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers | 2021-05-06 |
20210130782 | Engineered Exosomes to Detect and Deplete Pro-Tumorigenic Macrophages - CD206-positive M2 macrophage-targeting exosomes and methods of use thereof are provided. One embodiment provides a CD206-positive M2 macrophage-targeting exosome expressing a CD206 binding peptide and an Fc portion of IgG2b. In some embodiments, the CD206 binding peptide is encoded by a nucleic acid sequence having 95%, 99%, or 100% sequence identity to SEQ ID NO:2 and the IgG2b is encoded by a sequence having 95%, 99%, or 100% sequence identity to SEQ ID NO:6. | 2021-05-06 |
20210130783 | Device and Method for Breaking Down and Sizing Harvested Fat - A fat sizing device includes a first filter element and a second filter element. The first filter element has an exterior formed from a ceramic such as titanium nitride. The second filter element is positioned in series with the first filter element and has an exterior formed from an organic polymer such as a parylene. The first filter element has a first mesh size and the second filter element has a second mesh size different than the first mesh size and may be less than the first mesh size. | 2021-05-06 |
20210130784 | DE-DIFFERENTIATED FIBROBLAST-CONDITIONED MEDIA FOR STIMULATION OF DISC REGENERATION - Embodiments of the disclosure include methods and compositions for disc repair in a mammal using conditioned media (and/or one or more components therefrom) from fibroblasts that have been de-differentiated and cultured optionally with one or more particular conditions and/or compositions. In specific cases, fibroblasts that have been de-differentiated are exposed to hypoxia, histone deacetylase inhibitor(s), DNA methyltransferase inhibitor(s), or a combination thereof, and the conditioned media therefrom is provided in an effective amount to an individual. | 2021-05-06 |
20210130785 | MEDIUM FOR INDUCING DIFFERENTIATION OF STEM CELLS INTO MESODERMAL CELLS AND METHOD FOR PRODUCING MESODERMAL CELLS - The present invention provides a medium for inducing the differentiation of stem cells into mesodermal cells, the medium comprising a ROCK inhibitor, a bone morphogenetic protein (BMP), a fibroblast growth factor (FGF), and activin. The present invention also provides a method for producing mesodermal cells from stem cells using the medium, and method for producing cardiac progenitor cells or myocardiocytes from stem cells using the medium. The present invention furthermore provides a composition for assisting the induction of differentiation of stem cells into mesodermal cells, the composition comprising a ROCK inhibitor, a BMP, an FGF, and activin. The present invention additionally provides cell groups obtained by the above methods. | 2021-05-06 |
20210130786 | Assay for FGF2 Secretion and Signaling - A method for the identification of compounds modulating the biological activity of FGF2, the method comprising the use of target cells sensitive to the presence of FGF2 by obstructing proliferation, wherein the target cells constitutively express a first reporter in- the nucleus of living target cells. | 2021-05-06 |
20210130787 | MUSCLE DIFFERENTIATION-PROMOTING ACTIONS OF ERGOTHIONEINE, ASCORBIC ACID 2-GLUCOSIDE, ASCORBIC ACID, AND COMBINATION THEREOF - A culture medium and a culture medium additive which can promote myogenic differentiation and which contain ergothioneine, ascorbic acid 2-glucoside, ascorbic acid, or a combination thereof. A kit, pharmaceutical composition, food, and drink may also promote myogenic differentiation. | 2021-05-06 |
20210130788 | SUBSTRATES COATED WITH SELECTIVE CELL SEPARATION OR CELL CULTURE POLYMERS - Provided are a ligand-bearing substrate which has a surface at least partially coated with a polymer (P3) containing structural units represented by the formulae (1 | 2021-05-06 |
20210130789 | METHODS OF STROMAL CELL EXPANSION, USES AND MATERIALS RELATED THERETO - Disclosed herein are methods for expanding and potentiating intra-pancreatic tissue-derived mesenchymal stromal/stem cells in the presence of TNF-α, DMOG, or both, to obtain IPTD-MSCs having enhanced pro-angiogenic and/or anti-inflammatory properties. Also disclosed are therapeutic applications of these IPTD-MSCs. | 2021-05-06 |
20210130790 | Infected Cell Cultures - 3D cell cultures contain hepatic cells and are infected by a pathogen. Such cell cultures are prepared by, for example, inoculating a single-cell suspension containing hepatic cells expanded in a 2D culture in an agitation-based culture system. Next, the resulting cell culture is agitated at a given agitation rate. Then, the resulting 3D cell culture containing cell aggregates is incubated with a pathogen. | 2021-05-06 |
20210130791 | METHOD FOR ESTABLISHING COLORECTAL CANCER HK2 REPORTER GENE CELL LINE - The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene. Therefore, the expression level of the HK2 gene can be accurately determined by detecting a change in the expression level of the EGFP. The method for establishing a cell line in the present invention are simple, easy to implement and efficient, and a gene site can be precisely positioned. | 2021-05-06 |
20210130792 | ACTIVATION OF INNATE IMMUNITY FOR ENHANCED NUCLEAR REPROGRAMMING OF SOMATIC CELLS WITH mRNA - The nuclear reprogramming of somatic cells with mRNA encoding reprogramming factors is shown to be greatly accelerated by activation of innate immune responses in the somatic cell. Methods of activating innate immunity include activation of PKR, of toll-like receptors, e.g. TLR3, etc. In some embodiments the mRNA provides the activator of innate immunity. | 2021-05-06 |
20210130793 | METHOD FOR GRADUAL CONSTRUCTION OF REASSORTANT INFLUENZA VIRUS - Provided is a production method for reassortant influenza virus having genome segments of two or more kinds of influenza virus in the case where an antigenic strain and donor strain have similar antigenicities. The production method makes use of the first influenza virus containing an antigenic protein, the second influenza virus having an antigenic protein having antigenicity similar to that of the strain, and the third influenza virus having an antigenic protein having antigenicity different from that of the strain, and includes the steps of: coculturing the strain and the strain by infecting a host therewith, to produce reassortant influenza viruses; selecting influenza virus having the antigenic protein from the viruses; then coculturing the strain and the selected strain by infecting a host therewith; and selecting influenza virus having the antigenic protein from reassortant influenza viruses produced from the strain and the strain. | 2021-05-06 |
20210130794 | ADENO-ASSOCIATED VIRUS COMPOSITIONS FOR RESTORING PAH GENE FUNCTION AND METHODS OF USE THEREOF - Provided herein are adeno-associated virus (AAV) compositions that can restore phenylalanine hydroxylase (PAH) gene function in cell. Also provided are methods of use of the AAV compositions, and packaging systems for making the AAV compositions. | 2021-05-06 |
20210130795 | METHODS AND COMPOSITIONS FOR PRODUCING VITAMIN K DEPENDENT PROTEINS - The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells. | 2021-05-06 |
20210130796 | GCNT2/I-BRANCHING AS A BIOMARKER OF MELANOMA PROGRESSION - The present invention provides methods and composition for diagnosis, prognosis, prevention and/or treatment of cancers such as melanomas. The subject invention provides biomarkers and methods for assessing the severity of a cancer/tumor and for monitoring the progressing of a cancer/tumor. The biomarkers include glycosylation-rerated genes and molecules affected by the glycosylation-rerated genes. The compositions according to the subject invention regulate malignancy-associated pathways and alter melanoma signaling, growth, and survival. | 2021-05-06 |
20210130797 | Sucrose Phosphorylase Mutant with Improved Enzyme Activity and Construction Method Thereof and Use Thereof - The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from | 2021-05-06 |
20210130798 | LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2021-05-06 |
20210130799 | PRODUCTION OF CYCLIC ADENYLATES AND THEIR USE AS ALLOSTERIC REGULATORS - A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein. | 2021-05-06 |
20210130800 | SYSTEMS, METHODS, AND COMPOSITIONS FOR TARGETED NUCLEIC ACID EDITING - The present disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the present disclosure provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof. | 2021-05-06 |
20210130801 | Mannanase Variants and Polynucleotides Encoding Same - The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2021-05-06 |
20210130802 | Novel endolysin polypeptide - The invention relates to the field of medicine, specifically to the field of treatment of conditions associated with | 2021-05-06 |
20210130803 | SUBTILASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. | 2021-05-06 |
20210130804 | KNOCKOUT OF A MUTANT ALLELE OF AN ELANE GENE - Methods for inactivating in a cell a mutant allele of the elastase, neutrophil expressed gene (ELANE gene) gene having a mutation associated with severe congenital neutropenia (SCN) or cyclic neutropenia (CyN) wherein the mutant allele is selected from the group consisting of the ELANE mutants set forth in Table 1, the method comprising
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20210130805 | ADENOSINE DEAMINASE BASE EDITORS AND METHODS OF USING SAME TO MODIFY A NUCLEOBASE IN A TARGET SEQUENCE - The disclosure provides compositions comprising novel adenosine base editors (e.g., ABE8) that have increased efficiency and methods of using these adenosine deaminase variants for editing a target sequence. | 2021-05-06 |
20210130806 | ENGINEERED BACTERIA EXPRESSING RACEMASE FOR TREATING DISEASES ASSOCIATED WITH HYPERAMMONEMIA - Disclosed herein are bacteria engineered to treat diseases associated with hyperammonemia and methods of use thereof. Specifically, the bacteria are engineered to comprise a racemase to enable the detection of non-naturally occurring metabolites as an indication that the engineered bacteria are effectively converting ammonia. | 2021-05-06 |
20210130807 | KIT, SYSTEM, AND METHOD FOR NUCLEIC ACID EXTRACTION FROM SAMPLES - Kits, systems and methods for extracting nucleic acid, for example DNA or RNA, from a sample. The kits, systems and methods having a first syringe containing a lysis and preservative reagent, at least one second syringe containing an alcohol reagent, a third syringe containing a quantity of nucleic acid-soluble, nuclease-free water, a collection tube; and a filtering device. | 2021-05-06 |
20210130808 | Antigen Binding Molecules and Methods of Use Thereof - Isolated antigen binding molecules that specifically binds to a molecule comprising an amino acid sequence selected from the group consisting of GGGS (SEQ ID NO: 1), GGGGS (SEQ ID NO: 46) and related sequences are provided. The antigen binding molecules may be used in the methods provided herein. | 2021-05-06 |
20210130809 | EVOLUTION-GUIDED MULTIPLEXED DNA ASSEMBLY OF DNA PARTS, PATHWAYS AND GENOMES - The invention relates to a process for assembling DNA parts into multi-kilo base long synthetic DNA constructs. The process generates multiple, synonymous DNA parts in parallel and selects in a combinatorial assembly approach for those sequence variants with the best synthesis and assembly feasibility. DNA parts are sequence optimized and partitioned into synonymous variant designs that serve as redundant building units for higher order DNA assembly. The major stages of the process are: computational partitioning and synonymous recoding of the DNA design, DNA synthesis of sequence variants pools, serial PGR to isolate sets of DNA parts and higher order assembly. As the higher-order assembly does no longer depends on successful synthesis of each DNA part, large-scale DNA designs can be quickly completed allowing for cost-effective and highly parallelised assembly of synthetic bio-designs. | 2021-05-06 |
20210130810 | DEEP LEARNING ENABLED SPATIAL OPTICAL BARCODES FOR POOLED LIBRARY SCREENS - Disclosed herein include systems, methods, and compositions for determining perturbations in single cells or performing integrated measurements using, for example, plasmids each comprising (i) a perturbation gRNA with a guide region targeting a chromosome sequence and (ii) barcode gRNAs each with a guide region targeting predetermined spatial region of the genome and zero, one, or more optical detection probe binding sites. | 2021-05-06 |
20210130811 | HIGH THROUGHPUT DISCOVERY OF NEW GENES FROM COMPLEX MIXTURES OF ENVIRONMENTAL MICROBES - Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes. | 2021-05-06 |
20210130812 | DNA EXTRACTION METHOD USING MICROWAVE FOR NEXT GENERATION SEQUENCING AND USE THEREOF - Disclosed are a method for DNA extraction in a sample for next generation sequencing (NGS) and a method of constructing a NGS library using the extracted DNA. The method for DNA extraction includes: preparing a mixture by mixing a biological sample with a buffer; applying microwaves to the mixture; and recovering DNA. The method of constructing a NGS library includes: extracting DNA according to the method for DNA extraction; amplifying a target DNA using primers; and purifying the amplified product and subjecting the purified product to library pooling. | 2021-05-06 |
20210130813 | ANTIBODY LIBRARIES - The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity. | 2021-05-06 |
20210130814 | STRUCTURED SUBSTRATES FOR IMPROVING DETECTION OF LIGHT EMISSIONS AND METHODS RELATING TO THE SAME - A structured substrate includes a substrate body having an active side. The substrate body includes reaction cavities that open along the active side and interstitial regions that separate the reaction cavities. The structured substrate includes an ensemble amplifier positioned within each of the reaction cavities. The ensemble amplifier includes a plurality of nanostructures configured to at least one of amplify electromagnetic energy that propagates into the corresponding reaction cavity or amplify electromagnetic energy that is generated within the corresponding reaction cavity. | 2021-05-06 |
20210130815 | Evaluating Genomic Variation Using Repetitive Nucleic Acid Sequences - Systems and methods for evaluating genomic variation include utilizing tailed primers targeting repetitive genomic regions to amplify multiple regions throughout a genome. | 2021-05-06 |
20210130816 | THERMOSTABLE REVERSE TRANSCRIPTASE - Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate. | 2021-05-06 |
20210130817 | Gene Editing System and Gene Editing Method - The present invention relates to a CRISPR/Cas system comprising guide RNAs and a Cas protein, wherein the guide RNAs are composed of the following two parts: the guide RNAs are capable of respectively binding to two sequences of target sequences having SEQ ID NO: 2, SEQ NO: 3 and SEQ ID NO: 4 in eukaryotic B2M genes. The system exhibits a relatively high single-gene knockout rate for B2M. The present invention further relates to a gene editing method for T cells using the system, which method provides an efficient, safe and simple process for producing universal T cells. | 2021-05-06 |
20210130818 | Compositions and Methods for Enhancement of Homology-Directed Repair Mediated Precise Gene Editing by Programming DNA Repair with a Single RNA-Guided Endonuclease - The present invention includes compositions and methods for enhancing homology directed repair (HDR) and/or reducing non-homologous end joining (NHEJ) in a cell following CRISPR-mediated editing. | 2021-05-06 |
20210130819 | INCREASING RNAi EFFICIENCY THROUGH SINGLE NUCLEOTIDE MISMATCHES - Methods and compositions for increasing RNAi efficiency through single nucleotide mismatches. | 2021-05-06 |
20210130820 | METHOD FOR PRODUCING NUCLEIC ACID MOLECULE, BIOMATERIAL, AND METHOD FOR PRODUCING BIOMATERIAL - The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of: | 2021-05-06 |
20210130821 | CHIRAL CONTROL - The present invention relates to chirally controlled oligonucleotides, chirally controlled oligonucleotide compositions, and the method of making and using the same. The invention specifically encompasses the identification of the source of certain problems with prior methodologies for preparing chiral oligonucleotides, including problems that prohibit preparation of fully chirally controlled compositions, particularly compositions comprising a plurality of oligonucleotide types. In some embodiments, the present invention provides chirally controlled oligonucleotide compositions. In some embodiments, the present invention provides methods of making chirally controlled oligonucleotides and chirally controlled oligonucleotide compositions. | 2021-05-06 |
20210130822 | ANTISENSE OLIGOMER COMPOUNDS - A modified antisense oligonucleotide of about 10 to about 40 nucleobases is disclosed. The oligonucleotide comprises a targeting sequence having a region complementary to at least one string of three or more identical contiguous nucleobases in a target sequence, wherein the target sequence comprises at least one additional nucleobase compared to the region of the targeting sequence and the at least one additional nucleobase has no complementary nucleobase in the region of the targeting sequence, and wherein the targeting region complementary to the at least one string of three or more identical contiguous nucleobases is internal to the targeting sequence. | 2021-05-06 |
20210130823 | COMPOSITIONS AND METHODS - Provided herein are oligomeric compounds with conjugate groups. In certain embodiments, the oligomeric compounds are conjugated to N-Acetylgalactosamine. | 2021-05-06 |
20210130824 | COMPOSITIONS AND METHODS FOR GENE EDITING BY TARGETING FIBRINOGEN-ALPHA - Provided include compositions, methods, and systems for modulating the expression, function, and/or activity of a target gene, for example a blood-clotting protein such as Factor VIII (FVIII), in a cell by genome editing. Also provided include compositions, methods, and systems for treating a subject having or suspected of having a disorder or health condition, e.g., hemophilia A, employing ex vivo and/or in vivo genome editing. | 2021-05-06 |
20210130825 | ISCHEMIC-LESION-SITE-SPECIFIC GENE THERAPY - The present invention provides an antisense nucleic acid medicine that can modulate expression of a target transcriptional product in an ischemic site of a subject. The present invention also provides a composition for modulating expression of a target transcriptional product in an ischemic site of a subject, having a nucleic acid complex formed by annealing together a first nucleic acid strand having an antisense oligonucleotide region with respect to the target transcriptional product, and a lipid-conjugated second nucleic acid strand having a complementary region that is complementary to at least part of the first nucleic acid strand. | 2021-05-06 |
20210130826 | METHODS OF MODULATING ANTISENSE ACTIVITY - Disclosed herein are methods for increasing antisense oligonucleotide activity in a cell by modulating autophagy of the cell. In certain embodiments, a compound comprising an antisense oligonucleotide is co-administered to a subject with an autophagy modulator. | 2021-05-06 |
20210130827 | TYPE V CRISPR-CAS BASE EDITORS AND METHODS OF USE THEREOF - This invention relates to Type V CRISPR-Cas effector proteins, deaminases, and fusion and recruiting nucleic acid constructs thereof. The invention further relates methods of targeted nucleic acid modification utilizing the same. | 2021-05-06 |
20210130828 | GIN RECOMBINASE VARIANTS - The present disclosure provides a Gin recombinase catalytic domain variant and a zinc finger recombinase comprising a Gin recombinase catalytic domain variant operatively linked to a zinc finger nucleotide binding domain and methods for modifying the genome of a cell or to treat a disorder in a subject by using said zinc finger recombinase protein. | 2021-05-06 |
20210130829 | Nucleic Acid Compounds for Binding Growth Differentiation Factor 11 - Described herein are aptamers capable of binding to growth differentiation factor 11 (GDF11) protein; compositions comprising a GDF11 binding aptamer with a GDF11 protein; and methods of making and using the same. | 2021-05-06 |
20210130830 | APTAMERS SPECIFIC FOR TLR-4 AND USES THEREOF - The invention relates to a nucleic acid aptamer with the capability of binding specifically to a and inhibiting TLR-4, to a complex comprising said aptamer and a functional group, as well as to pharmaceutical compositions thereof. The invention also relates to uses and methods for detecting TLR-4 and to uses and methods for inhibiting TLR-4. Finally, the invention also relates to an aptamer for use in manufacturing a drug for the treatment of a pathology characterized by an increase in expression of TLR4 and/or an increase in activation of TLR-4. | 2021-05-06 |
20210130831 | LUNG-SPECIFIC DRUG DELIVERY SYSTEM CONSISTING OF OLIGONUCLEOTIDE POLYMERS AND BIOCOMPATIBLE CATIONIC PEPTIDES FOR THE PREVENTION OR TREATMENT OF PULMONARY FIBROSIS AND USE THEREOF - The present invention relates to a lung-specific drug delivery system consisting of oligonucleotide polymers and biocompatible cationic peptides for the prevention or treatment of pulmonary fibrosis, and a use thereof. The drug delivery system according to the present invention can be specifically accumulated in the lungs and absorbed into lung fibrotic cells to knock down TGF-β, thereby preventing or treating pulmonary fibrosis. | 2021-05-06 |
20210130832 | METHODS OF GENE THERAPY - Compositions and methods for gene and/or cell editing are provided. | 2021-05-06 |
20210130833 | BACTERIAL DEFENSE SYSTEMS AND METHODS OF IDENTIFYING THEREOF - Engineered systems comprising components of defense systems identified in prokaryotes are provided. | 2021-05-06 |
20210130834 | INCREASED NUCLEIC-ACID GUIDED CELL EDITING IN YEAST - The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods. | 2021-05-06 |
20210130835 | COMPOSITIONS AND METHODS FOR RNA-ENCODED DNA-REPLACEMENT OF ALLELES - This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants. | 2021-05-06 |
20210130836 | PROTEIN FOR REGULATING INSECT RESISTANCE IN ARABIDOPSIS THALIANA AND ENCODING GENE AND USE THEREOF - The present disclosure relates to use of a lectin receptor-like kinase LecRK-IX.1 as a protein for regulating insect resistance of | 2021-05-06 |
20210130837 | Plant Vectors, Compositions and Uses Relating Thereto - The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant. | 2021-05-06 |
20210130838 | SYSTEMS AND METHODS FOR PLANT GENOME EDITING USING CAS 12a ORTHOLOGS - A non-naturally occurring heterologous CRISPR-Cas12a genomic editing system is described, including or encoding at least one Cas12a ortholog endonuclease selected from among Lb5Cas12a, CMaCas12a, BsCas12a, BoCas12a, M1Cas12a, Mb2Cas12a, MbCas12a TsCas12a, and MAD7 endonucleases. The ortholog endonucleases enable genomic editing of plants at low temperatures, e.g., below 25° C. The CRISPR-Cas12a system can be employed for targeting PAM sites such as TTN, TTV, TTTV, NTTV, TATV, TATG, TATA, YTTN, GTTA, and GTTC, utilizing corresponding gRNAs, and is readily adapted to multiplexed editing operations of plants such as rice and other food crops. | 2021-05-06 |
20210130839 | WHEAT WITH INCREASED RESISTANT STARCH LEVELS - A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes. | 2021-05-06 |
20210130840 | INCREASING PLANT GROWTH AND YIELD BY USING A DUF2996 DOMAIN-CONTAINING PROTEIN - Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding DUF2996 domain-containing protein s, polypeptides encompassing DUF2996 domain-containing protein s, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided. | 2021-05-06 |
20210130841 | NUCLEOTIDE SEQUENCES AND CORRESPONDING POLYPEPTIDES CONFERRING MODULATED GROWTH RATE AND BIOMASS IN PLANTS GROWN IN SALINE AND OXIDATIVE CONDITIONS - The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline and/or oxidative stress conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline and/or oxidative stress conditions with respect to wild-type plants grown under similar conditions. | 2021-05-06 |
20210130842 | Plant Regulatory Elements and Uses Thereof - The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use. | 2021-05-06 |
20210130843 | ROQ1 PROVIDES RESISTANCE TO BOTH XANTHOMONAS AND PSEUDOMONAS IN PLANTS - Provided herein is a non- | 2021-05-06 |
20210130844 | STERILE MUTANT AND TWO LINE BREEDING SYSTEM - Disclosed herein are nuclear male sterile | 2021-05-06 |
20210130845 | COMPOSITIONS AND METHODS FOR CHIMERIC LIGAND RECEPTOR (CLR)-MEDIATED CONDITIONAL GENE EXPRESSION - Disclosed are composition comprising (a) an inducible transgene construct, comprising a sequence encoding an inducible promoter and a sequence encoding a transgene, and (b) a receptor construct, comprising a sequence encoding a constitutive promoter and a sequence encoding an exogenous receptor, wherein, upon integration of the construct of (a) and the construct of (b) into a genomic sequence of a cell, the exogenous reporter is expressed, and wherein the exogenous reporter, upon binding a ligand, transduces an intracellular signal that targets the inducible promoter of (a) to modify gene expression. Methods for introducing compositions into cells and the use of the resultant cells in adoptive cell therapies are also provided. | 2021-05-06 |
20210130846 | GENETIC MODIFICATION OF RATS - Compositions and methods are provided for making rat pluripotent and totipotent cells, including rat embryonic stem (ES) cells. Compositions and methods for improving efficiency or frequency of germline transmission of genetic modifications in rats are provided. Such methods and compositions comprise an in vitro culture comprising a feeder cell layer and a population of rat ES cells or a rat ES cell line, wherein the in vitro culture conditions maintain pluripotency of the ES cell and comprises a media having mouse leukemia inhibitory factor (LIF) or an active variant or fragment thereof. Various methods of establishing such rat ES cell lines are further provided. Methods of selecting genetically modified rat ES cells are also provided, along with various methods to generate a transgenic rat from the genetically modified rat ES cells provided herein. Various kits and articles of manufacture are further provided. | 2021-05-06 |