19th week of 2016 patent applcation highlights part 24 |
Patent application number | Title | Published |
20160130568 | THERMOPHILIC AND THERMOACIDOPHILIC GLYCOSYLATION GENES AND ENZYMES FROM ALICYCLOBACILLUS ACIDOCALDARIUS AND RELATED ORGANISMS, METHODS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 2016-05-12 |
20160130569 | A LAGLIDADG HOMING ENDONUCLEASE CLEAVING THE T CELL RECEPTOR ALPHA GENE AND USES THEREOF - Disclosed herein are compositions for inactivating the human TCR-alpha gene comprising engineered LAGLIDADG homing endonucleases (LHEs) and their derivatives, particularly derived from members of the \-Onul subfamily of LHEs. Polynucleotides encoding such endonucleases, vectors comprising said polynucleotides, cells comprising or having been treated with such endonucleases, and therapeutic compositions deriving therefore are also provided. | 2016-05-12 |
20160130570 | METHOD FOR TREATING ONCOLOGICAL DISEASES - A method to treat cancer and other malignant diseases, said method comprising parenterally administering an agent which destroys blood extracellular DNA into the systemic circulation of a cancer patient to slow down cancer growth. The agent is embodied in the form of a DNase enzyme and, more particularly, as a DNase I enzyme. Doses from 50,000-250,000,000 Kunitz units/day are administered for 5-360 days. | 2016-05-12 |
20160130571 | Alpha-Amylase from Bacillaceae Family Member - Disclosed are compositions and methods relating to an alpha-amylase from a Bacillaceae family member. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 2016-05-12 |
20160130572 | ALPHA AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2016-05-12 |
20160130573 | Method of Controlling a Polypeptide Modification Reaction - The invention relates to a method of controlling a polypeptide modification reaction, in particular but not exclusively, a method of controlling the activation of human factor VII (FVII) to produce human factor VII(a) (FVII(a)). The invention also relates to polypeptides obtainable by the polypeptide modification reaction and to pharmaceutical compositions comprising said polypeptides. | 2016-05-12 |
20160130574 | METHODS OF MEASURING GENE EXPRESSION IN FACS-SORTED CELLS - Improved methods of measuring gene expression in intracellularly immunostained FACS-sorted cell populations are provided. Exemplary methods involve fixing cells with formalin and permeabilizing with mild detergent in the presence of ribonucleoside vanadyl complex prior to FACS sorting, followed by RNA extraction in the presence of de-crosslinking agents. The resulting RNA is suitable for gene expression analysis. The method allows for analysis of the gene expression pattern specifically associated with any sortable cell population or subpopulation. | 2016-05-12 |
20160130575 | SCREENING OF NUCLEIC ACID AGENTS VIA PARTICLE DISPLAY - The present disclosure provides a method for identifying one or more nucleic acid agents, e.g., aptamers, having a desired property from a mixture of candidate nucleic acid agents. The method generally includes immobilizing the mixture of candidate nucleic acid agents onto particles, wherein only a subset of the candidate nucleic acid agents are immobilized on any one of the particles, and wherein the subset is present in multiple copies. The particles are exposed to a target, and particles including candidate nucleic acid agents having the desired property are isolated. In this way, one or more nucleic acid agents having the desired property may be identified. Related compositions and nucleic acid agents identified as having one or more desired properties are also provided. | 2016-05-12 |
20160130576 | COMPOSITIONS AND METHODS FOR DIRECTIONAL NUCLEIC ACID AMPLIFICATION AND SEQUENCING - The invention provides methods and compositions, including kits, for directional nucleic acid amplification and sequencing. The invention further provides methods and compositions for the construction of directional cDNA libraries. | 2016-05-12 |
20160130577 | NUCLEOTIDE SEQUENCE MOTIFS DIRECTING NUCLEIC ACID LOCATION TO EXTRACELLULAR VESICLES - The present invention discloses the use of isolated short sequence motifs capable of directing or packaging regulatory nucleic acids, preferably RNAs, into extracellular vesicles, preferably exosomes. This mechanism is enhanced by the binding of hnRNP family proteins, which are sumoylated, to such nucleic acid. In this sense, sumoylated hnRNPs directs the loading of nucleic acids into EVs through recognition of specific short motifs disclosed in the present invention. Additionally, the present invention discloses recombinant nucleic acids comprising such sequence motifs, EVs in turn comprising these recombinant nucleic acids, as well as the compositions, preferably pharmaceutical compositions comprising either the recombinant nucleic acids or the EVs of the invention. The identification of such motifs is a useful tool for use in genetic engineering and gene therapy. | 2016-05-12 |
20160130578 | REDUCED SIZE SELF-DELIVERING RNAI COMPOUNDS - The present invention relates to methods for in vivo administration of sd-rxRNA molecules. | 2016-05-12 |
20160130579 | ADIPOCYTE-TARGETING NON-VIRAL GENE DELIVERY SYSTEM - The present invention relates to a gene delivery system for targeting adipocytes and a treatment system for obesity and obesity-derived metabolic syndromes using the same and, more particularly, to a non-viral gene delivery system which directly targets a differentiated obesity (mature) adipocyte and contains an adipocyte targeting sequence (ATS)-arginine (R9) peptide. | 2016-05-12 |
20160130580 | RNA AMIDATES AND THIOAMIDATES FOR RNAI - The present disclosure relates to RNA amidates and thioamidates useful for RNA interference applications. The RNA amidates and thioamidates contain at least one internucleoside linkage chosen from ribo-N3′→P5′ phosphoramidate (NP) and ribo-N3′→P5′ thiophosphoramidate (NPS) linkages, and optionally further containing at least one covalently conjugated lipid moiety. Compositions comprising the amidates and thioamidates are disclosed, as are methods for their use in modulating gene expression. | 2016-05-12 |
20160130581 | TARGETING DOMAIN AND RELATED SIGNAL ACTIVATED MOLECULAR DELIVERY - Provided herein are signal activatable molecular constructs for enzyme-assisted delivery of molecules and related components, such as a sensor domain, compositions, methods and systems. | 2016-05-12 |
20160130582 | OLIGONUCLEOTIDE MODULATORS OF B-CELL CLL/LYMPHOMA 11A (BCL11A) AND USES THEREOF - The present invention provides, among other things, oligonucleotide modulators (e.g., inhibitors) of B cell lymphoma/leukemia 11A (BCL11A) and improved methods and composition for treating BCL11A-related diseases, disorders or conditions based on such modulators. | 2016-05-12 |
20160130583 | DOUBLE-STRANDED ANTISENSE NUCLEIC ACID WITH EXON-SKIPPING EFFECT - Disclosed are double-stranded antisense nucleic acid complexes that can efficiently alter the processing of RNA in a cell via an antisense effect, and methods for using the same. One method comprises contacting with the cell a double-stranded nucleic acid complex comprising: a first nucleic acid strand annealed to a second nucleic acid strand, wherein: the first nucleic acid strand comprises (i) nucleotides independently selected from natural DNA nucleotides, modified DNA nucleotides, and nucleotide analogs, (ii) no regions that have 4 or more consecutive natural DNA nucleotides, (iii) the total number of natural DNA nucleotides, modified DNA nucleotides, and nucleotide analogs in the first nucleic acid strand is from 8 to 100, and (iv) the first nucleic acid strand is capable of hybridizing to RNA inside of the cell; and the second nucleic acid strand comprises nucleotides independently selected from natural RNA nucleotides, modified RNA nucleotides, and nucleotide analogs. | 2016-05-12 |
20160130584 | TREATMENT OF ALPHA-L-IDURONIDASE (IDUA) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO IDUA - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Alpha-L-Iduronidase (IDUA), in particular, by targeting natural antisense polynucleotides of Alpha-L-Iduronidase (IDUA). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of IDUA. | 2016-05-12 |
20160130585 | APTAMERS FOR THE TREATMENT OF SICKLE CELL DISEASE - The present invention provides polynucleotide aptamers that selectively bind to and inhibit polymerization of sickle hemoglobin (HbS), pharmaceutical compositions comprising the same, methods of use for diagnostics and treatment of sickle cell disease, methods of use as capture reagents, and methods of rational drug design. | 2016-05-12 |
20160130586 | PREVENTIVE OR THERAPEUTIC AGENT FOR FIBROSIS - Provided is siRNA effective for the treatment of fibrosis and a pharmaceutical containing the siRNA. | 2016-05-12 |
20160130587 | INTERFERING RNA MOLECULES - The present invention is related to a ribonucleic acid comprising a double stranded structure whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and whereby said first stretch is at least partially complementary to a target nucleic acid, and the second strand comprises a second stretch of contiguous nucleotides whereby said second stretch is at least partially identical to a target nucleic acid, and whereby the double stranded structure is blunt ended. | 2016-05-12 |
20160130588 | METHOD FOR THE GENERATION OF POLYCYSTRONIC VECTORS - The present invention provides a method for generating polycystronic nucleic acid vectors, said method comprising the steps of a) providing a first nucleic acid vector, said first nucleic acid vector comprising: i) an origin of replication placed in front of ii) at least one first gene of interest functionally cloned within a first expression cassette, said first expression cassette comprising a promoter sequence as well as a termination sequence, said first gene of interest being located between said promoter sequence and said termination sequence, iii) a marker gene together with its termination sequence, said marker gene being situated downstream of a target sequence for a recombinase, wherein the promoter necessary for the expression of said marker gene in a host cell is absent from said first nucleic acid vector, and optionally iv) a first further marker gene which is different from the marker gene of iii), said first further marker gene being situated within said first expression cassette of ii) or within a further expression cassette, said first further marker gene allowing for the selection of cells comprising said first nucleic acid vector during the process of generating said first nucleic acid vector; b) providing a second nucleic acid vector, said second nucleic acid vector comprising i) an origin of replication placed in front of ii) at least one second gene of interest functionally cloned within a second expression cassette, said second expression cassette comprising a promoter sequence as well as a termination sequence, said second gene of interest being located between said promoter sequence and said termination sequence, iii) a promoter situated upstream of the same target sequence for a recombinase as present in front of the marker gene of iii) in the first nucleic acid vector, wherein said promoter is suitable for the expression in a host cell of said marker comprised in the first nucleic acid vector gene, and iv) optionally a second further marker gene which is different from the marker gene of iii), second further marker gene being situated within said second expression cassette of ii) or within a further expression cassette, said second further marker gene allowing for the selection of cells comprising said first nucleic acid vector during the process of generating said second nucleic acid vector; and c) contacting, under conditions suitable for a recombination to take place, a mixture of the first and the second nucleic acid vectors with a recombinase, which recombinase specifically recombines the target sequences of iii) which is situated downstream of the promoter of the second nucleic acid vector and upstream of the marker gene of the first nucleic acid vector, respectively. | 2016-05-12 |
20160130589 | FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY - The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions. | 2016-05-12 |
20160130590 | BACTERIAL XYLOSE ISOMERASES ACTIVE IN YEAST CELLS - Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate. | 2016-05-12 |
20160130591 | METHOD FOR IDENTIFYING SUBSTANCES WHICH PRIME CELLS FOR A STRESS RESPONSE AND CELLS FOR USE IN THIS METHOD - The present invention relates to a transgenic eukaryotic cell or non-human organism comprising one or more genetic modifications providing the activation of one or more signal transduction pathways which are involved in stress-induced gene expression and/or the pre-activation of one or more members of the transcriptional machinery and an expression cassette which comprises a nucleic acid sequence coding for a reporter protein under the control of a promoter the methylation of which increases upon priming for a stress response. The present invention also relates to a method for identifying substances which prime eukaryotic cells for a stress response by using this transgenic cell or organism. | 2016-05-12 |
20160130592 | Soybean Transformation Using HPPD Inhibitors as Selection Agents - The invention relates to methods for | 2016-05-12 |
20160130593 | ARTIFICIAL DNA SEQUENCE WITH OPTIMIZED LEADER FUNCTION IN 5'(5'-UTR) FOR THE OVER-EXPRESSION OF RECOMBINANT PROTEINS IN PLANTS AND METHOD FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN PLANTS - Artificial DNA of a 5′-UTR leader region, which artificial DNA is effective in increasing the expression of recombinant proteins in plants, and comprises, along the 5′→3′ direction, an Inr initiator site and a Kozak or Kozak-like consensus sequence, and also comprises, between the Inr initiator site and the Kozak or Kozak-like consensus sequence, a plurality of poly(CAA) and a plurality of poly(CT) regions, in the same number as the poly(CAA) regions wherein at least one, optionally each one, poly(CAA) region, in the 5′→3′ direction, is upstream of a poly(CT) region and at least one poly(CAA) region, in the 5′→3′ direction, is contiguous with a poly(CT) region, wherein the artificial DNA provides the absence of A/T-rich motifs, the absence of trinucleotide elements ATT, the absence of trinucleotide elements CTG and the absence of homopolymeric tracts, that is, sequences consisting of more than 3, optionally more than 4, identical nucleotides. | 2016-05-12 |
20160130594 | SYNTHETIC BI-DIRECTIONAL PLANT PROMOTER - This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a | 2016-05-12 |
20160130595 | SYNTHETIC BI-DIRECTIONAL PLANT PROMOTER - This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a | 2016-05-12 |
20160130596 | METHOD OF PRODUCING LEAFY BIOMASS - A method for producing leafy biomass from undifferentiated plant cells, the method comprising providing undifferentiated plant cells, contacting them with an agent that promotes differentiation of the cells into leafy tissue and growing the cells in a temporary liquid immersion culture system. This method of the invention may be used to produce polypeptides, and natural medicinal products, and can be used to capture carbon dioxide. | 2016-05-12 |
20160130597 | Regulation of Gene Expression - The invention provides isolated polynucleotides comprising sequences encoding a u ORF peptides and variants and fragments thereof. The invention also provides constructs and vectors containing the polynucleotides. The invention further provides cells, plant cells and plants transformed with the polynucleotides and constructs. The invention also provides methods of using the polynucleotides to control expression of operably linked polynucleotides. The invention also provides methods of manipulating GDP-L-Galactose phosphorylase (GGP) expression and ascorbate production in plants utilising the polynucleotides of the invention. | 2016-05-12 |
20160130598 | PEPPER WITH INCREASED TOTAL CONTENT OF TERPENOIDS - The present invention relates to a pepper plant ( | 2016-05-12 |
20160130599 | MODIFIED DIATOMS FOR BIOFUEL PRODUCTION - The invention provides engineered diatoms and methods of producing oil using diatoms. The invention also provides methods of modifying the lipids quantity and/or quality produced by diatom organisms through genome engineering. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured from such modified diatoms. | 2016-05-12 |
20160130600 | Soybean Seed and Oil Compositions and Methods of Making Same - Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed. | 2016-05-12 |
20160130601 | NUCLEIC ACID SEQUENCES ENCODING TRANSCRIPTION FACTORS REGULATING ALKALOID BIOSYNTHESIS AND THEIR USE IN MODIFYING PLANT METABOLISM - Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis. | 2016-05-12 |
20160130602 | MEANS AND METHODS FOR YIELD PERFORMANCE IN PLANTS - This disclosure relates to the field of plant molecular biology; more particularly to the field of agriculture; even more particularly to the field of improving the yield of plants. This disclosure provides chimeric genes and constructs that can be used to enhance the yield in plants and crops. | 2016-05-12 |
20160130603 | GENE ENCODING ENZYME THAT OXIDIZES POSITION 16 OF STEROID SKELETON AND PLANT IN WHICH EXPRESSION LEVEL OF THE GENE IS LOWERED - This invention provides a plant belonging to the family Solanaceae that does not produce glycoalkaloids. This invention concerns a protein having activity of an enzyme that oxidizes position 16 of the steroid skeleton of a plant belonging to the family Solanaceae, a novel plant in which a gene encoding such protein is suppressed, and a method for producing and testing such plant. | 2016-05-12 |
20160130604 | RICE CULTIVAR CL172 - A rice cultivar designated CL172 is disclosed. The invention relates to the seeds of rice cultivar CL172, to the plants of rice CL172, to methods for producing a rice plant produced by crossing the cultivar CL172 with itself or another rice variety, and to methods for controlling weeds in the vicinity of plants of rice cultivar CL172, which comprises increased resistance to acetohydroxyacid synthase-inhibiting herbicides. The invention further relates to hybrid rice seeds and plants produced by crossing the cultivar CL172 with another rice cultivar. | 2016-05-12 |
20160130605 | MDA-9/SYNTENIN PROMOTER TO IMAGE AND TREAT METASTATIC CANCER CELLS - Recombinant vectors in which expression of one or more elements (e.g. genes required for viral replication, detectable imaging agents, therapeutic agents, etc.) is driven by an mda- | 2016-05-12 |
20160130606 | FREEZE-DRIED POLYELECTROLYTE COMPLEXES THAT MAINTAIN SIZE AND BIOLOGICAL ACTIVITY - The present invention relates to a polyelectrolyte complex composition comprising a polymer, a nucleic acid molecule, a lyoprotectant, and a buffer. The polyelectrolyte complex composition preserving the biological activities of the polyelectrolyte complex following freeze-drying and rehydration. | 2016-05-12 |
20160130607 | AGENTS FOR IMPROVED DELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS - New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. | 2016-05-12 |
20160130608 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2016-05-12 |
20160130609 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2016-05-12 |
20160130610 | PROCESSES FOR THE ACIDIC, ANAEROBIC CONVERSION OF HYDROGEN AND CARBON OXIDES TO OXYGENATED ORGANIC COMPOUND - Processes for the bioconversion of syngas to oxygenated organic compound are disclosed that reliably, cost-effectively and efficiently supply sulfur nutrient to microorganisms contained in acidic, aqueous fermentation menstrua. In the processes of this invention, basic, aqueous solution used to maintain the pH of the aqueous fermentation menstruum is used to remove hydrogen sulfide from the off-gas from the fermentation menstruum for recycle to the fermentation menstruum. | 2016-05-12 |
20160130611 | Microbial Synthesis Of Aldehydes And Corresponding Alcohols - An improved process for alcohol production includes microbial fermentation using a genetically modified microorganism to produce substantial quantities of aldehydes that are stripped from the fermentation medium and condensed. So produced aldehydes are converted in an ex vivo process to corresponding alcohols. | 2016-05-12 |
20160130612 | Integration of a Polynucleotide Encoding a Polypeptide that Catalyzes Pyruvate to Acetolactate Conversion - The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. | 2016-05-12 |
20160130613 | 3HP TOLERANCE - Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway. | 2016-05-12 |
20160130614 | METHOD OF ENHANCED BIOPRODUCTION - Bio-based renewable 3-hydroxypropionic acid (3-HP) may be produced through fermentation processes utilizing genetically modified microorganisms such as, for example, genetically modified | 2016-05-12 |
20160130615 | Acyl-ACP Thioesterase - An acyl-ACP thioesterase consisting of an amino acid sequence of the 72 | 2016-05-12 |
20160130616 | METHODS OF PRODUCING OMEGA-HYDROXYLATED FATTY ACID DERIVATIVES - The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation. | 2016-05-12 |
20160130617 | Coryneform Bacterium Transformant and Process for Producing Aniline Using The Same - Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture. | 2016-05-12 |
20160130618 | Method for Manufacturing Useful Substance - A method for producing an objective substance is provided. An objective substance is produced by culturing a microorganism which has been modified so that the activity of a dicarboxylic acid exporter protein is reduced in a medium, and collecting the objective substance from the medium. | 2016-05-12 |
20160130619 | Sphingomonas Strains Producing Greatly Increased Yield of PHB-Deficient Sphingan (Diutan) - PHB-deficient | 2016-05-12 |
20160130620 | BIOCATALYST FOR SIMULTANEOUSLY DEGRADING LIGNIN AND CELLULOSE, AND METHOD FOR MANUFACTURING HYDROLYSATE AND BIOFUEL USING THE SAME - The present disclosure relates to a method for simultaneously degrading lignin and cellulose and for boosting effect on the cellulase activity using a specific catalyst. Since the present disclosure allows for the preparation of sugars by degrading not only lignin but also cellulose and hemicellulose using the enzymes which were previously known only as lignin-degrading biocatalysts, it provides the advantage that the preparation of a hydrolysate as a source material for the production of biofuels or biochemicals from lignocellulosic biomass can be simplified and facilitated. As a result, the present disclosure can reduce enzyme cost and can provide improved production efficiency by simplifying the biofuel production process. | 2016-05-12 |
20160130621 | METHOD FOR PREPARING SIALIC ACID DERIVATIVE - The present invention relates to a method for preparing a sialic acid derivative characterized by performing both of a process for preparing CMP-N-acetylneuraminic acid using N-acetyl-D-glucosamine and a process for preparing the sialic acid (neuraminic acid) derivative that combines a sialic acid with a galactose derivative or a lactose derivative, together, in one reactor. According to the method for preparing a sialic acid derivative of the present invention, expensive cytidine 5′-monophosphate (CMP) is capable of being recycled in a reactor, such that an amount of the CMP introduced into the reactor may be reduced, and the sialic acid derivative is capable of being prepared at a significantly high efficiency by using cheap N-acetyl-D-glucosamine, and pyruvate as substrates. | 2016-05-12 |
20160130622 | ACTINOMYCETE INTEGRATIVE AND CONJUGATIVE ELEMENT FROM ACTINOPLANES SP. SE50/110 AS PLASMID FOR GENETIC TRANSFORMATION OF RELATED ACTINOBACTERIA - The present invention is directed to an innate DNA sequence within the complete genome sequence of | 2016-05-12 |
20160130623 | Methods for Amplification of Nucleic Acids Utilizing Clamp Oligonucleotides - The present invention provides methods of amplifying a target nucleic acid utilizing a clamp oligonucleotide comprising a first target-binding region on the 3′-terminus and a second target-binding region on the 5′-terminus and tether region in between. The tether region may comprise a variety of user-defined sequences or elements that allow for further manipulation of the target nucleic acid. Such as, for example, capture followed by amplification, identification and/or sequencing. The target-binding regions bind to the target nucleic acid, the 3′-terminus functions as a primer to initiate extension across the target nucleic acid sequence and ligation of the gap results in formation of a circularized nucleic acid. This circular template can be used in a variety of processes, including amplification and sequencing. | 2016-05-12 |
20160130624 | HARVEST OPERATIONS FOR RECOMBINANT PROTEINS - The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO | 2016-05-12 |
20160130625 | METHODS FOR PRODUCING MELANIN AND INORGANIC FERTILIZER FROM FERMENTATION LEACHATES - Melanin or inorganic fertilizers are produced from fermentation leachates or from low-cost nutrient-rich solutions. The method for producing the melanin or inorganic fertilizer comprises repetitive trophic cycling in the controlled conditions of primary and secondary bioreactors. Nutrients are cycled between microorganisms such as bacteria, yeast and fungi and black soldier fly larvae, | 2016-05-12 |
20160130626 | Expression of Natively Secreted Polypeptides Without Signal Peptide - The present invention relates to methods of recombinantly producing a natively secreted polypeptide, the method comprising the steps of providing a microorganism host cell comprising an exogenous polynucleotide encoding a natively secreted polypeptide without a translationally fused signal peptide; cultivating the microorganism host cell under conditions conducive to the expression of the polypeptide and, optionally, recovering the polypeptide, as well as microorganisms, certain polynucleotides, expression constructs and protease substitution variants. | 2016-05-12 |
20160130627 | IMPROVED PRODUCTION PROCESSES OF HAEMATOCOCCUS CELL COMPONENTS - The technology disclosed herein relates to novel methods and compositions for the production processes of algae cell components from algae of the genus | 2016-05-12 |
20160130628 | MICROORGANISM COMPRISING GENE FOR CODING ENZYME INVOLVED IN PRODUCING RETINOID AND METHOD FOR PRODUCING RETINOID BY USING SAME - The present invention relates to a microorganism comprising a gene for coding an enzyme involved in producing retinoid and a method for producing retinoid by using the same, and more specifically, to: a microorganism capable of mass-producing retinoid at a remarkable efficiency by comprising a gene for coding an enzyme involved in producing retinoid; and a method for producing retinoid by using the same. | 2016-05-12 |
20160130629 | ENDOGENOUS AUTO-FLUORESCENT BIOLOGICAL MARKERS FOR ASSESSING A BIOLOGICAL PARAMETER OF A CELL - The present application related to the use of endogenous fluorescent biological markers to determine a parameter of a cell in a liquid Because the techniques provided herein provide accurate results in a relatively short amount of time, the methods described herein can be used to monitor and optimize cell culture online as well determine the presence of a cellular contamination in a cell suspension. | 2016-05-12 |
20160130630 | METHOD FOR DETECTING CAMPYLOBACTER - A selective isolation medium that is not influenced by existence of ESBL-producing bacteria and can clearly detect | 2016-05-12 |
20160130631 | MASS SPECTROMETRIC DIAGNOSIS OF SEPTICEMIA - The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from bloodstream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour. | 2016-05-12 |
20160130632 | METHODS AND DEVICES FOR DIAGNOSIS OF PARTICLES IN BIOLOGICAL FLUIDS - Methods for determining whether certain compounds, in particular crystals, are present in a sample of a biological fluid that indicates an individual has a particular disease or condition, such as but not limited to gout, pseudogout or urinary tract stones. In some embodiments, the methods include the steps of digestion and filtration of a sample of synovial fluid in order to isolate, if present, monosodium urate monohydrate (MSU), calcium pyrophosphate dihydrate (CPPD), or calcium phosphate crystals from the sample, wherein the filtrate is analyzed with a Raman device to ascertain the presence and type of the crystals. Devices for performing steps of the method are disclosed. | 2016-05-12 |
20160130633 | METHOD AND APPARATUS FOR AMPLIFYING DNA FRAGMENT BASED ON CONTROLLING PH CHANGE OF REACTION SOLUTION - The present invention provides a method and apparatus based on a DNA fragment amplified by changing the pH value of a control reaction solution. Specifically, the present invention provides a method for nucleic acid amplification, comprising the following steps: (a) under conditions of pH 10-14 alkalinity, melting of a double-stranded nucleic acid molecule; (b) under conditions of pH 5-8 neutrality and near-neutrality, annealing of the melted nucleic acid molecule and a primer; and in the presence of a nucleic acid polymerase, causing the primer bound to the single-stranded nucleic acid molecule to extend to form an amplified double-stranded nucleic acid molecule. | 2016-05-12 |
20160130634 | PROCESS FOR THE SYNTHESIS OF A cDNA IN A SAMPLE IN AN ENZYMATIC REACTION - This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture. | 2016-05-12 |
20160130635 | ISOLATION OF NUCLEIC ACIDS - Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool. | 2016-05-12 |
20160130636 | QUANTITATING HIGH TITER SAMPLES BY DIGITAL PCR - The present invention provides systems, devices, methods, kits, and compositions for nucleic acid analysis using digital PCR. In particular, methods are provided to analyze high titer samples that cannot be divided into a sufficient number of partitions containing zero nucleic acid molecules per partition to allow for Poisson analysis (digital PCR analysis). | 2016-05-12 |
20160130637 | DUAL LABELING METHODS FOR MEASURING CELLULAR PROLIFERATION - The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog. | 2016-05-12 |
20160130638 | METHODS FOR THE DIAGNOSIS OF BACTERIAL VAGINOSIS - The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis. | 2016-05-12 |
20160130639 | System and Method for Detection of Nucleic Acids - Embodiments provide detection systems and methods for detecting the presence of a nucleic acid in one or more samples. In a detection method, a sample and one or more nucleic acid probes are introduced into a channel. A first potential difference is applied across the length of the channel in a first direction, and a first electrical property value is detected. Subsequently, a second potential difference is applied across the length of the channel in a second opposite direction, and a second electrical property value is detected. Presence or absence of a nucleic acid in the channel is determined based on a comparison between the first and second electrical property values. | 2016-05-12 |
20160130640 | CARTRIDGE FOR PERFORMING ASSAYS IN A CLOSED SAMPLE PREPARATION AND REACTION SYSTEM - In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence. | 2016-05-12 |
20160130641 | HIGHLY SENSITIVE METHOD FOR DETECTING LOW FREQUENCY MUTATIONS - The disclosed edge-blocker oligonucleotide based AS-NEPB-PCR method amplifies allele specific DNA (or RNA) while dramatically blocking amplification of wild type (WT) DNA (or RNA). The AS-NEPB-PCR design allows ready modification of an existing PCR reaction setup with an edge-blocker oligonucleotide together with an allele specific primer complementary to the mutant sequence to achieve allele specific amplification. The method simplifies assay optimization procedures and achieved sensitivity sufficient to detect a signal present at 0.1% level with close to 100% specificity, which is useful in detecting SNP or genetic mutations. The method was used to detect three different genetic mutations in cancer, in KRAS, BRAF, and EGFR, with three different types of modified edge-blocker oligonucleotides (phosphate, inverted dT and amino-C7). It was possible to detect one copy of mutant DNA in 1000-copy of normal DNA background of a heterogeneous sample, and was far more sensitive than the other blocking method. | 2016-05-12 |
20160130642 | METHOD, MICROREACTOR AND APPARATUS FOR CARRYING OUT REAL-TIME NUCLEIC ACID AMPLIFICATION - A method for carrying out nucleic acid amplification, includes providing a reaction chamber, accommodating an array of nucleic acid probes at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution into the reaction chamber, wherein the solution contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes and of the primers so that a hybridization temperature of the probes is higher than an annealing temperature of the primers, whereby hybridization and annealing take place in respective separate temperature ranges. | 2016-05-12 |
20160130643 | ACCURATE IN VITRO COPYING OF DNA METHYLATION - A method of copying a methylated nucleic acid molecule is provided. The method includes copying a nucleic acid molecule into a plurality of nucleic acid molecules; and contacting the plurality of nucleic acid molecules with a DNA methyltransferase enzyme and an E3 ubiquitin ligase. The method results in the copying of the methylated nucleic acid molecule. | 2016-05-12 |
20160130644 | THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS - The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety. | 2016-05-12 |
20160130645 | ULTRAFAST SEQUENCING OF BIOLOGICAL POLYMERS USING A LABELED NANOPORE - Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer. | 2016-05-12 |
20160130646 | METHODS AND COMPOSITIONS FOR SEQUENCING MODIFIED NUCLEIC ACIDS - Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid. | 2016-05-12 |
20160130647 | SIGNAL CONFINEMENT SEQUENCING (SCS) AND NUCLEOTIDE ANALOGUES FOR SIGNAL CONFINEMENT SEQUENCING - Novel fluorescent nucleotide analogues are provided herein. Also provided herein are methods of using the nucleotide analogues in sequencing-by-synthesis and signal confinement methods. | 2016-05-12 |
20160130648 | DEEP SEQUENCING OF PERIPHERAL BLOOD PLASMA DNA AS A RELIABLE TEST FOR CONFIRMING THE DIAGNOSIS OF MYELODYSPLASTIC SYNDROME - Methods are provided for treating, managing, diagnosing and monitoring myelodysplastic syndrome and other hematologic malignancies. These methods comprise the next generation sequencing analysis conducted on cell-free DNA from peripheral blood plasma or serum. | 2016-05-12 |
20160130649 | Single Cell Nucleic Acid Detection and Analysis - Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided. | 2016-05-12 |
20160130650 | NANOWIRE-BASED SYSTEM FOR ANALYSIS OF NUCLEIC ACIDS - System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids. | 2016-05-12 |
20160130651 | Biological Analysis Systems, Devices, And Methods - A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other. | 2016-05-12 |
20160130653 | POLYNUCLEOTIDE PROBE, METHOD FOR DETECTING A TARGET NUCLEIC ACID BY USING THE SAME AND KIT COMPRISING THE SAME - The present invention provides a method for detecting a target nucleic acid that comprises a step of providing a sample; contacting the sample with a polynucleotide probe comprising a first sequence and a second sequence complementary to the target nucleic acid; and adding a nuclease for cleaving the second sequence of the polynucleotide probe. The present invention further provides a polynucleotide probe for detecting a target nucleic acid that comprises a first sequence and a second sequence complementary to the target nucleic acid. Moreover, the present invention provides a kit for detecting a target nucleic acid. | 2016-05-12 |
20160130654 | SYSTEMS AND METHODS FOR DIAGNOSING AND TREATING CANCER - Embodiments of the invention provide a method detecting and treating cancer, such as lung cancer. In some aspects, the method may include detecting cancer in a subject, which may comprise assessing the expression of a marker in a sample from the subject. For example, the marker may comprise Mcl-1. In some embodiments, if the subject is diagnosed as having cancer, the method may further provide administering a therapeutically effective amount of a substance that reduces the expression level of Mcl-1 to the subject and then administering a treatment modality that is known to treat the cancer. | 2016-05-12 |
20160130655 | MUTATIONS IN THE BCR-ABL TYROSINE KINASE ASSOCIATED WITH RESISTANCE TO STI-571 - The invention described herein relates to novel genes and their encoded proteins, termed Mutants Associated with Resistance to STI-571 (e.g., T315I Bu-Abl), and to diagnostic and therapeutic methods and compositions useful in the management of various cancers that express MARS. The invention further provides methods for identifying molecules that bind to and/or modulate the functional activity of MARS. | 2016-05-12 |
20160130656 | METHODS FOR EVALUATING LUNG CANCER STATUS - The disclosure in some aspects provides methods of determining the likelihood that a subject has lung cancer based on the expression of informative-genes. In other aspects, the disclosure provides methods for determining an appropriate diagnostic intervention plan for a subject based on the expression of informative-genes. Related compositions and kits are provided in other aspects of the disclosure. | 2016-05-12 |
20160130657 | METHOD OF DIAGNOSING NEOPLASMS - The present invention relates generally to a nucleic acid molecule, the RNA and protein expression profiles of which are indicative of the onset, predisposition to the onset and/or progression of a large intestine neoplasm. More particularly, the present invention is directed to a nucleic acid molecule, the expression profiles of which are indicative of the onset and/or progression of a colorectal neoplasm, such as an adenoma or an adenocarcinoma. The expression profiles of the present invention are useful in a range of applications including, but not limited to, those relating to the diagnosis and/or monitoring of colorectal neoplasms, such as colorectal adenomas and adenocarcinomas. Accordingly, in a related aspect the present invention is directed to a method of screening a subject for the onset, predisposition to the onset and/or progression of a large intestine neoplasm by screening for modulation in the expression profile of said nucleic acid molecule markers. | 2016-05-12 |
20160130658 | METHODS FOR DETECTING AND TREATING VARIANTS OF SEBORRHEIC KERATOSES - Disclosed herein are assays and methods for distinguishing a keratoacanthoma from squamous cell carcinoma. Methods for diagnosing a keratoacanthoma are also provided. Methods for treating variants of seborrheic keratoses (i.e., keratoacanthoma, acanthosis nigricans, or epidermal nevus) are also provided. | 2016-05-12 |
20160130659 | COMPOSITIONS AND METHODS FOR TREATING CANCER - The instant invention relates to methods for the treatment of B-cell lymphomas and leukemia by administering a SYK inhibitor. In another embodiment, the invention relates to a method for treating a patient diagnosed with a B-cell lymphoma or leukemia, comprising administering a SYK inhibitor, wherein the B-cells of said patient to be treated are characterized by elevated expression levels of CD86. | 2016-05-12 |
20160130660 | COMPOSITIONS AND METHODS FOR MULTIMODAL ANALYSIS OF CMET NUCLEIC ACIDS - Described herein are methods and assays relating to the detection of cMET alterations (e.g. variations in copy number and expression level, and/or the presence of mutations, including point mutations). Existing methods are limited in their clinical usefulness by, e.g., limited sensitivity, inter-lab discordance, or inability to provide the necessary multiplex ability. The methods and assays provided herein permit multimodal, multiplex assaying for faster, more cost-effective testing and screening of patients, permitting improved healthcare. | 2016-05-12 |
20160130661 | METHODS FOR DETECTING PROSTATE CANCER - The present disclosure relates to methods for detecting a prostate cancer. Certain embodiments of the present disclosure provide a method of detecting a prostate cancer in a subject, the method comprising detecting a marker selected from an endosomal associated marker and/or a lysosomal associated marker from the subject. | 2016-05-12 |
20160130662 | NOVEL GENOMIC MARKER OF METASTATIC PROSTATE CANCER - The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer in a patient. In a specific embodiment, a method for predicting metastasis in a prostate cancer patient comprises the steps of (a) genotyping the ASPN D repeat domain length in both alleles of the patient using a polymerase chain reaction; (b) predicting metastasis in the patient if the ASPND repeat domain length is 13 in one allele and 14 in the other allele or have at least one allele with 14 D repeats as compared to other common allelic genotypes; and (c) predicting no metastasis in the patient if the ASPN D repeat domain length is 13 in both alleles as compared to other allelic genotypes. | 2016-05-12 |
20160130663 | METHOD FOR PREDICTING RESPONSE TO CANCER TREATMENT - It has been found that BRG1 and BRM have a synthetic-lethal relationship, and that a treatment for inhibiting BRM is a promising approach for treating a BRG1-deficient cancer having no mutation in known therapeutic target genes. Moreover, it has also been revealed that, in this therapeutic strategy, a BRM inhibitor may be administered to a cancer patient after the selection based on BRG1 function suppression, thus enabling an efficient treatment based on the companion diagnosis. | 2016-05-12 |
20160130664 | DETERMINING TUMOR LOAD AND BIALLELIC MUTATION IN PATIENTS WITH CALR MUTATION USING PERIPHERAL BLOOD PLASMA - Compositions and fragment length analysis methods are provided for detecting CALR mutations and determining tumor load in patients with myeloproliferative neoplasms. | 2016-05-12 |
20160130665 | PROGNOSTIC METHODS AND SYSTEMS FOR CHRONIC LYMPHOCYTIC LEUKEMIA - The present invention provides systems useful for risk stratification of chronic lymphocytic leukemia (CLL) patients. The systems can include a microarray and a decision tree having steps for stratification of one or more CLL patients into prognostic groups. The invention further provides methods for risk stratification of CLL patients. The methods can include detecting the presence of alterations, such as copy number alterations, in sample genetic material from each of one or more CLL patients and then stratifying the one or more CLL patients into prognostic groups. | 2016-05-12 |
20160130666 | SIMULTANEOUS DETECTION OF MUTATIONAL STATUS AND GENE COPY NUMBER - The present invention provides compositions and methods for simultaneously detecting mutational status and gene copy number. In particular, the present invention provides simultaneous measurement of gene copy number and detection of the L858R and Exon 19 del mutations in a tissue sample. | 2016-05-12 |
20160130667 | Method For The Selection Of A Long-Term Producing Cell - Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide. | 2016-05-12 |
20160130668 | Detection of Lethality Gene for Improved Fertility in Mammals - Oligonucleic acid molecules comprising a SNP site at a position corresponding to position 7480 of the bovine signal transducer and activator of transcription (STAT5A) coding sequence (SEQ ID NO: 1). Also disclosed are an array or a kit comprising the same, a method for detecting the SNPs, a method for progeny testing of mammals, a method for increasing human and non-human mammal pregnancy rate in natural and artificial reproduction processes. Further provided are cattle breeding methods for improved milk production traits. | 2016-05-12 |