21st week of 2022 patent applcation highlights part 28 |
Patent application number | Title | Published |
20220162598 | OLIGONUCLEOTIDE COMPOSITIONS AND METHODS THEREOF - Among other things, the present disclosure relates to chirally controlled oligonucleotides of select designs, chirally controlled oligonucleotide compositions, and methods of making and using the same. In some embodiments, a provided chirally controlled oligonucleotide composition provides different cleavage patterns of a nucleic acid polymer than a reference oligonucleotide composition. In some embodiments, a provided chirally controlled oligonucleotide composition provides single site cleavage within a complementary sequence of a nucleic acid polymer. In some embodiments, a chirally controlled oligonucleotide composition has any sequence of bases, and/or pattern or base modifications, sugar modifications, backbone modifications and/or stereochemistry, or combination of these elements, described herein. | 2022-05-26 |
20220162599 | METHODS AND COMPOSITIONS FOR MODULATING SPLICING OF ALTERNATIVE INTRONS - Provided herein are methods and compositions for modulating expression of a target protein or a target RNA by modulating splicing pre-mRNA and for treating diseases or conditions associated with expression level of the target protein or the target RNA. | 2022-05-26 |
20220162600 | Compositions for the Multiplexed Detection of Viruses - This specification discloses compositions of matter and processes that allow the detection of RNA from coronaviruses and other RNA viruses, in particular, compositions and processes that have the capacity to detect in multiplexed form many RNA targets within individual viruses, targets from multiple viruses, and other RNA molecules that can be used as positive controls. | 2022-05-26 |
20220162601 | HIGH THROUGHPUT GENE EDITING SYSTEM AND METHOD - The present disclosure provides CRISPC-Cas systems for the creating mutated genes within cells within wells of an addressable well plate. | 2022-05-26 |
20220162602 | POLYNUCLEOTIDE AGENTS TARGETING COMPLEMENT COMPONENT C5 AND METHODS OF USE THEREOF - The invention relates to polynucleotide agents targeting the complement component C5 gene, and methods of using such polynucleotide agents to inhibit expression of C5 and to treat subjects having a complement component C5-associated disease, e.g., paroxysmal nocturnal hemoglobinuria. | 2022-05-26 |
20220162603 | COMPOSITIONS COMPRISING ALTERNATING 2'-MODIFIED NUCLEOSIDES FOR USE IN GENE MODULATION - The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression. | 2022-05-26 |
20220162604 | ANTISENSE NUCLEIC ACIDS - Provided is a drug that allows highly-efficient skipping of exon 51 in the human dystrophin gene. The present invention provides an antisense oligomer which enables exon 51 in the human dystrophin gene to be skipped. | 2022-05-26 |
20220162605 | ANTISENSE OLIGOMERS FOR TREATMENT OF CONDITIONS AND DISEASES - Alternative splicing events in SCN1A gene can lead to non-productive mRNA transcripts which in turn can lead to aberrant protein expression, and therapeutic agents which can target the alternative splicing events in SCN1A gene can modulate the expression level of functional proteins in Dravet Syndrome patients and/or inhibit aberrant protein expression. Such therapeutic agents can be used to treat a condition caused by SCN1A, SCN8A or SCN5A protein deficiency. | 2022-05-26 |
20220162606 | NRL EXPRESSION REDUCING OLIGONUCLEOTIDES, COMPOSITIONS CONTAINING THE SAME, AND METHODS OF THEIR USE - Disclosed are oligonucleotides having a nucleobase sequence with at least 6 contiguous nucleobases complementary to an equal-length portion within an NRL target nucleic acid. The oligonucleotides may be single-stranded or double-stranded. Also disclosed are pharmaceutical compositions containing the oligonucleotides and methods of their use. | 2022-05-26 |
20220162607 | NUCLEIC ACID COMPLEX FOR MODULATING IHH EXPRESSION - This invention provides a nucleic acid complex and a method for decreasing the expression levels of mRNA and a protein of the IHH gene in animals. The nucleic acid complex for decreasing the expression levels of mRNA and a protein of the IHH gene serves as an IHH-specific inhibitor and it is thus useful for elucidation of IHH gene functions. In addition, an IHH-specific inhibitor is useful for treatment, prevention, amelioration, or delaying of the progression of fibrosis and other fibrotic diseases. Since the nucleic acid complex according to the present invention specifically inhibits IHH gene expression, it is useful for a patient who is in need of treatment, prevention, amelioration, or delaying of the progression of fibrosis and other fibrotic diseases. | 2022-05-26 |
20220162608 | EXTENDED DICER SUBSTRATE AGENTS AND METHODS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION - The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs. | 2022-05-26 |
20220162609 | MODULATORY POLYNUCLEOTIDES - The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of modulatory polynucleotides. | 2022-05-26 |
20220162610 | NOVEL RNA TRANSCRIPT - An alternatively spliced intronic sequence is disclosed, the splicing of which can be induced in the presence of a small molecule, as described herein. | 2022-05-26 |
20220162611 | INHIBITOR OF MIR-129 AND USES THEREOF - The present invention relates to an inhibitor of miR-129, relative compounds and pharmaceutical compositions for use in the treatment and/or prevention of amyotrophic lateral sclerosis and Alzheimer's disease. The invention also relates to a method for the diagnosis and/or prognosis of Alzheimer's disease in a subject or to identify a subject at risk to develop amyotrophic lateral sclerosis or Alzheimer's disease and to a method for the measuring the efficacy of a therapy for amyotrophic lateral sclerosis or for Alzheimer's disease and relative kits. | 2022-05-26 |
20220162612 | SYNTHETIC TRANSFER RNA WITH EXTENDED ANTICODON LOOP - The invention relates to a synthetic transfer RNA with an extended anticodon loop. The invention provides a synthetic suppressor transfer RNA useful for the treatment of a genetic disease like neurofibromatosis associated with a frameshift mutation. The synthetic transfer RNA comprises an extended anticodon loop having a four-nucleotide anticodon or a five-nucleotide anticodon. | 2022-05-26 |
20220162613 | ANGPTL4 OLIGONUCLEOTIDES INFLUENCING THE REGULATION OF THE FATTY ACID METABOLISM - An ANGPTL4 inhibitor consists of an oligonucleotide has 12 to 22 nucleotides. At least one of the nucleotides is modified, and the oligonucleotide hybridizes with a nucleic acid sequence of human and/or muse ANGPTL4 and inhibits the expression of ANGPTL4. A pharmaceutical composition can include the ANGPTL4 inhibitor and a pharmaceutically acceptable carrier, excipient diluent, or a combination of these. | 2022-05-26 |
20220162614 | RNAi-MEDIATED INHIBITION OF HIF1A FOR TREATMENT OF OCULAR ANGIOGENESIS - RNA interference is provided for inhibition of HIF1A mRNA expression for treating patients with ocular angiogenesis, particularly for treating retinal edema, diabetic retinopathy, sequela associated with retinal ischemia, posterior segment neovascularization (PSNV), and neovascular glaucoma, and for treating patients at risk of developing such conditions. | 2022-05-26 |
20220162615 | METHODS FOR REDUCING ATAXIN-2 EXPRESSION - Provided herein are methods for decreasing Ataxin-2 mRNA expression. Such methods are useful to ameliorate symptoms of Ataxin-2 associated diseases. Such Ataxin-2 associated diseases include amyotrophic lateral sclerosis (ALS). Such symptoms include loss of motor function, reduced CMAP amplitude, denervation, and loss of motor neurons. | 2022-05-26 |
20220162616 | DUAL INHIBITION OF PLEXIN-B1 AND PLEXIN-B2 - The present invention relates to a composition comprising an inhibitor of plexin-B1 and an inhibitor of plexin-B2, wherein the inhibitor of plexin-B1 and/or the inhibitor of plexin-B2 is/are preferably selected from (i) an inhibitor of the nucleic acid molecule encoding plexin-B1 and/or plexin-B2 selected from a small molecule, an aptamer, a siRNA, a shRNA, a miRNA, a morpholino, a ribozyme, an antisense nucleic acid molecule, a CRISPR-Cas9-based construct, a CRISPR-Cpf1-based construct, a meganuclease, a zinc finger nuclease, and a transcription activator-like (TAL) effector (TALE) nuclease, and/or (ii) an inhibitor of the plexin-B1 and/or plexin-B2 protein selected from a small molecule, an antibody or antibody mimetic, an aptamer, wherein the antibody mimetic is preferably selected from affibodies, adnectins, anticalins, DARPins, avimers, nanofitins, affilins, Kunitz domain peptides and Fynomers®. | 2022-05-26 |
20220162617 | METHODS OF MODULATING SLC7A11 PRE-MRNA TRANSCRIPTS FOR DISEASES AND CONDITIONS ASSOCIATED WITH EXPRESSION OF SLC7A11 - Described are methods for enhancing exon skipping in a pre-mRNA of interest, comprising contacting the pre-mRNA with interfering oligonucleotides such as antisense, siRNA, and miRNA. The exon skipping methods are associated with methods of treating a variety of diseases and conditions, including cancer. | 2022-05-26 |
20220162618 | ENGINEERED MRNA SEQUENCES AND USES THEREOF - The present disclosure relates to a series of engineered mRNA sequences and methods of use for improving protein expression. | 2022-05-26 |
20220162619 | PREPARATION OF WHEAT CYSTEINE PROTEASE TRITICAIN-ALPHA PRODUCED IN SOLUBLE FORM AND METHOD OF PRODUCING SAME - The invention relates to the field of molecular biology, preparative biochemistry, biotechnology, and biopharmacology, namely to the creation of recombinant proteins of the family of wheat ( | 2022-05-26 |
20220162620 | Applications of Streptococcus-derived Cas9 nucleases on minimal Adenine-rich PAM targets - Applications of a | 2022-05-26 |
20220162621 | Methods For Polynucleotide Integration Into The Genome Of Bacillus Using Dual Circular Recombinant DNA Constructs And Compositions Thereof - Methods and compositions are provided for integrating genes of interest into the genome of a | 2022-05-26 |
20220162622 | A NOVEL VECTOR FOR GENE TRANSFER AND GENE COPY PROLIFERATION - The invention relates to a circular vector for plant transformation comprising: a first tethering nucleic acid and a second tethering nucleic acid; a nucleic acid encoding a polynucleotide of interest (POI); a nucleic acid comprising an origin of replication; and two or more nucleic acids encoding replicon proteins and methods for using the same. Also provided are plants produced by the methods of the invention and products produced from the plant. | 2022-05-26 |
20220162623 | COMPOSITIONS AND METHODS FOR INCORPORATION OF DNA INTO THE GENOME OF AN ORGANISM - Methods and compositions are provided which allow for genetic modification of host cells, including plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacteria and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell and comprises a transformation vector comprising a recombinant DNA construct. | 2022-05-26 |
20220162624 | OPTIMAL MAIZE LOCI - As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences. | 2022-05-26 |
20220162625 | METHODS TO IMPROVE SITE-DIRECTED INTEGRATION FREQUENCY - The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells. | 2022-05-26 |
20220162626 | Modulation of Transgene Expression - Compositions and methods of modulating, for example reducing, recombinant insecticidal protein expression in male reproductive cells and/or tissues of transgenic plants are disclosed. In particular, novel recombinant DNA constructs useful in such methods, as well as transgenic plants, cells, and seeds containing such recombinant DNA constructs are provided. The recombinant DNA constructs and the transgenic plants, cells, and seeds containing such constructs provide a greatly improved way to minimize any potential risks that may be associated with expression of insecticidal proteins in male reproductive tissues. | 2022-05-26 |
20220162627 | HIGH FRUCTAN CEREAL PLANTS - The invention provides cereal plants having a high level of fructan useful for the production of a range of food, beverage, nutraceutical and pharmaceutical products. The invention provide, methods of producing high-fructan products from plants modified to comprise a reduced level of an endogenous polypeptide with starch synthase activity, and products so produced. In some embodiments, plants are modified by introduction of an agent such as a nucleic acid molecule which down regulates endogenous starch synthase II gene expression. | 2022-05-26 |
20220162628 | WAXY CORN - The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain. | 2022-05-26 |
20220162629 | Process for the Production of Polyunsaturated Fatty Acids in Transgenic Organisms - The present invention relates to a process for the production of polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which encode polypeptides with Δ5-elongase activity. Advantageously, these nucleic acids can be expressed in the organism together with further nucleic acids which encode polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Especially advantageous are nucleic acids which encode Δ6-desaturases, Δ5-desaturases, Δ4-desaturases and/or Δ6-elongases. These desaturases and elongases are advantageously derived from | 2022-05-26 |
20220162630 | INBRED TRANSGENIC CANOLA LINE NS-B50027-4 AND SEEDS THEREOF - The present embodiments relate to inbred transgenic canola line NS-B50027-4; seeds and oils obtained from NS-B50027-4; and progeny derived from NS-B50027-4. In particular, NS-B50027-4 is a true-breeding canola line capable of producing at least 5% DHA in its seed oil. | 2022-05-26 |
20220162631 | Isolated Novel Nucleic Acid and Protein Molecules from Soybeans and Methods of Using Those Molecules to Generate Transgenic Plants With Enhanced Agronomic Traits - This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants. | 2022-05-26 |
20220162632 | DELAYED HARVEST OF SHORT STATURE CORN PLANTS - Methods for delayed harvesting of corn fields are provided herein. These methods provide an extended, flexible period of time to harvest corn. The methods allow growers to harvest their corn at the optimal time for drying down or accessing seed, without increasing the risk of losing yield to lodging. | 2022-05-26 |
20220162633 | USE OF SOYBEAN PROTEIN KINASE GENE GMSTK_IRAK - The present disclosure provides use of a soybean protein kinase gene GmSTK_IRAK and belongs to the technical field of plant genetic engineering. PCR is used to clone the soybean GmSTK_IRAK gene, a transgene and gene editing technology is used to obtain GmSTK_IRAK-over-expressing transgenic plants and GmSTK_IRAK gene-silenced transgenic plants. GmSTK_IRAK-over-expressing soybeans can greatly improve phosphorus absorption and utilization efficiency, biomass and yield, while GmSTK_IRAK gene-silenced soybeans can reduce phosphorus absorption and utilization efficiency, biomass and yield. The soybean protein kinase gene GmSTK_IRAK can be used as a target gene to be introduced into plants to regulate the balance of phosphorus metabolism in transgenic plants, and is of great significance for cultivating new soybean varieties with high phosphorus efficiency. | 2022-05-26 |
20220162634 | NOVEL RESISTANCE GENES ASSOCIATED WITH DISEASE RESISTANCE IN SOYBEANS - The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using genes derived from wild | 2022-05-26 |
20220162635 | CHIMERIC INSECTICIDAL PROTEINS - IRDIG35563 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes IRDIG35563 variants, fragments and analogs. | 2022-05-26 |
20220162636 | Inducible AAV System Comprising Cumate Operator Sequences - The invention relates to nucleic acid molecules, vectors and plasmids comprising AAV cap genes and rep genes, wherein the cap and rep genes are both operably-associated with an inducible promoter which comprises one or more cumate operator (CuO) sequences. The invention further relates to producer and packaging cell lines which are useful in the production of Adeno-Associated Virus (AAV) particles. | 2022-05-26 |
20220162637 | AAV MUTANT HAVING BRAIN-TARGETING PROPERTY - The present invention provides a nucleic acid which encodes an adeno-associated virus (AAV) capsid protein mutant that contains a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID Nos. 15 to 62 or a peptide comprising an amino acid sequence produced by substituting, deleting, inserting and/or adding one or several amino acid residues in an amino acid sequence selected from the group consisting of SEQ ID Nos. 15 to 62; DNA comprising the nucleic acid; a cell harboring the DNA; and a method for producing the cell. | 2022-05-26 |
20220162638 | VIRAL VECTOR CONSTRUCTS FOR DELIVERY OF NUCLEIC ACIDS ENCODING CYTOKINES AND USES THEREOF FOR TREATING CANCER - The present disclosure provides the gene therapy compositions comprising vectors (e.g., viral vectors) suitable for delivery of nucleic acids encoding immunomodulatory proteins or functional fragments thereof, and methods of using the same. Certain aspects of the disclosure are directed to an adeno-viral vector (AAV) delivery of nucleic acids encoding two or more immunomodulatory proteins or functional fragments thereof to a tumor. | 2022-05-26 |
20220162639 | TREATMENT FOR RESTORING UREAGENESIS IN CARBAMOYL PHOSPHATE SYNTHETASE 1 DEFICIENCY - Carbamoyl phosphate synthetase 1 (CPS1) deficiency is a metabolic disorder of the liver that results m abnormal nitrogen metabolism. To illustrate the ability of gene therapy to treat CPS1 deficiency, two adeno-associated viruses encoding portions of a codon optimized CPS1 were generated and tested in a conditional CPS1 knock out mouse model. When administered to mice having knocked out endogenous CPS1 expression, mice from this model demonstrate homologous recombination and reconstitution of the codon optimized CPS1 gene, expression of the CPS1 protein and the associated control of plasma ammonia following the administered AAVs comprising the CPS1 gene sequences. While all control mice perish, the mice in this model live and have normal behavior. As there is no effective therapy for human patients with the CPS1 disorder, this invention can address this unmet need for these patients. | 2022-05-26 |
20220162640 | HYBRID PROMOTERS FOR MUSCLE EXPRESSION - The present invention relates to hybrid promoters to drive gene expression in muscles. | 2022-05-26 |
20220162641 | FACTOR H VECTORS AND USES THEREOF - Aspects of the disclosure relate to compositions and methods for expressing a Factor H protein (or a variant thereof) in a cell or subject. In some embodiments, the disclosure provides isolated nucleic acids and rAAVs comprising a transgene encoding a Factor H protein variant and one or more regulatory sequences. In some embodiments, compositions described herein are useful for treating subjects having diseases associated with Factor H deficiency. | 2022-05-26 |
20220162642 | PLASMID SYSTEM - The present invention relates to two-plasmid systems, helper plasmids, and/or vector plasmids for producing recombinant AAV (rAAV) vectors. The invention further relates to methods using, or uses of, the two-plasmid systems, helper plasmids and/or vector plasmids of the invention. | 2022-05-26 |
20220162643 | OPTIMIZED PHENYLANANINE HYDROXYLASE EXPRESSION - A lentiviral vector system for expressing a lentiviral particle is disclosed. The lentiviral vector system includes a therapeutic vector. The lentiviral vector system produces a lentiviral particle that encodes a codon-optimized PAH for upregulating PAH expression in the cells of a subject afflicted with phenylketonuria (PKU). | 2022-05-26 |
20220162644 | DELIVERY VECTORS AND PARTICLES FOR EXPRESSING CHIMERIC RECEPTORS AND METHODS OF USING THE SAME - The present disclosure provides delivery vectors for expressing a chimeric receptor in a monocytic cell, such as a macrophage or dendritic cell. The chimeric receptor may specifically bind to a particular antigen or target molecule, such as an immune checkpoint protein or OX40. The disclosed delivery vectors can be used to treat cancer in a subject by expressing in vivo a chimeric receptor on the surface of the subject's monocytic cells. | 2022-05-26 |
20220162645 | CELL PORATION AND TRANSFECTION APPARATUSES - In example implementations, an apparatus is provided. The apparatus includes a channel, a thermal inkjet (TIJ) resistor, and a transfection chamber. The TIJ resistor is to apply heat to a cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated. | 2022-05-26 |
20220162646 | NUCLEIC ACID-GUIDED NUCLEASES - Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods. | 2022-05-26 |
20220162647 | METHOD FOR INDUCING TARGETED MEIOTIC RECOMBINATIONS - The present invention relates to a fusion protein comprising a Cas9 domain and a Spo11 domain, as well as the use of this protein to induce targeted meiotic recombinations in a eukaryotic cell. | 2022-05-26 |
20220162648 | COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITING - The present disclosure provides methods of introducing site-specific mutations in a target cell and methods of determining efficacy of enzymes capable of introducing site-specific mutations. The present disclosure also provides methods of providing a bi-allelic sequence integration, methods of integrating of a sequence of interest into a locus in a genome of a cell, and methods of introducing a stable episomal vector in a cell. The present disclosure further provides methods of generating a human cell that is resistant to diphtheria toxin. | 2022-05-26 |
20220162649 | NOVEL NUCLEIC ACID MODIFIERS - The present inventions generally relate to site-specific delivery of nucleic acid modifiers and includes novel DNA-binding proteins and effectors that can be rapidly programmed to make site-specific DNA modifications. The present inventions also provide synthetic all-in-one genome editor (SAGE) systems comprising designer DNA sequence readers and a set of small molecules that induce double-strand breaks, enhance cellular permeability, inhibit NHEJ and activate HDR, as well as methods of using and delivering such systems. | 2022-05-26 |
20220162650 | RECOMBINANT MICROORGANISM INCLUDING GENETIC MODIFICATION THAT INCREASES ACTIVITY OF NITRIC OXIDE REDUCTASE AND METHOD OF REDUCING CONCENTRATION OF NITRIC OXIDE IN SAMPLE BY USING THE SAME - A recombinant microorganism including a genetic modification that increases activity of nitric oxide reductase in the recombinant microorganism, a composition for reducing a concentration of nitric oxide in a sample, the composition including the recombinant microorganism, and a method of reducing a concentration of nitric oxide in a sample, are disclosed. | 2022-05-26 |
20220162651 | Methods and Compositions for Cell-Free Biological Reactions - Compositions and methods disclosed herein relate to improved in vitro cell-free systems for various biological reactions. In one aspect, a composition of the present disclosure includes: a cell-free extract derived from an organism; a nucleic acid; and an organelle that is heterologous to the organism. | 2022-05-26 |
20220162652 | METHOD FOR PREPARING AN EXTRACT CONTAINING GALLIC ACID, AQUEOUS CONCENTRATE CONTAINING GALLIC ACID, AND FOODSTUFF AND FOOD SUPPLEMENT CONTAINING THE AQUEOUS CONCENTRATE - A method for preparing an extract containing gallic acid includes performing an extraction treatment on plants or plant constituents containing tannins by means of water or an aqueous system in the presence of the enzyme tannase to obtain a product mixture containing gallic acid. Further disclosed is a method for preparing an extract containing gallic acid, comprising performing an extraction treatment on plants or plant constituents containing tannins by means of a mixture containing a polar organic solvent and water to obtain a product mixture containing polyphenols, separating the plants or plant constituents from the product mixture to obtain a liquid extract, removing the polar organic solvent from the liquid extract, and treating the aqueous extract with the enzyme tannase to obtain an aqueous system containing gallic acid. In addition, the disclosure includes an aqueous concentrate containing gallic acid and a foodstuff or food supplement comprising the aqueous concentrate. | 2022-05-26 |
20220162653 | Preparation of (R)-3-Hydroxybutyric Acid or Its Salts by One-Step Fermentation - The subject invention relates to a genetically modified microorganism comprising a (R)-3-hydroxybutyric acid pathway and being able of producing (R)-3-hydroxybutyric acid, and a method of preparing (R)-3-hydroxybutyric acid or a salt thereof using the genetically modified microorganism. | 2022-05-26 |
20220162654 | METHOD FOR PREPARING AMINES FROM CARBONYL COMPOUNDS BY TRANSAMINASE REACTION UNDER SALT PRECIPITATION - Described herein is a method for preparing an amino salt compound, the method including: i) providing a carbonyl compound; ii) reacting the carbonyl compound provided according to (i) in the presence of a transaminase with ii-a) at least one primary amine; and ii-b) at least one carboxylic acid; thereby obtaining a mixture including an at least partially crystallized amino salt compound including a cation and a carboxylate anion based on the at least one carboxylic acid added according to (ii-b). Also described herein is an amino salt compound obtained or obtainable by the method and to the amino salt compound, and a composition including a) an amine of general formula (IIa); and b) at least one carboxylic acid of general formula (III). | 2022-05-26 |
20220162655 | ENGINEERED BIOSYNTHETIC PATHWAYS FOR PRODUCTION OF L-HOMOCYSTEINE BY FERMENTATION - The present disclosure describes the engineering of microbial cells for fermentative production of L-homocysteine and provides novel engineered microbial cells and cultures, as well as related L-homocysteine production methods. | 2022-05-26 |
20220162656 | METHODS OF IMPROVING PRODUCTION OF MORPHINAN ALKALOIDS AND DERIVATIVES - Methods and systems are provided for producing codeinone within an engineered non-plant cell. The method comprises, within the engineered non-plant cell, producing a thebaine product. The method also comprises, within the engineered non-plant cell, contacting the thebaine product with an enzyme having thebaine 6-O-demethylase activity, thereby producing a neopinone product. Additionally, the method comprises, within the engineered non-plant cell, contacting the neopinone product with a neopinone isomerase, thereby producing a codeinone product. | 2022-05-26 |
20220162657 | GLUCOAMYLASES AND METHODS OF USE, THEREOF - Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein. | 2022-05-26 |
20220162658 | GLYCOSYLATION OF PLANT EXTRACT BY WHOLE CELL BIOCONVERSION - In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells. | 2022-05-26 |
20220162659 | CELL-FREE PRODUCTION OF RIBONUCLEIC ACID - This invention relates to in vitro production of nucleic acids, particularly RNAs and specifically messenger RNAs (mRNA). | 2022-05-26 |
20220162660 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5′ domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3′ domain, wherein the primer is not extendable by a polymerase, and wherein the 3′ domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions. | 2022-05-26 |
20220162661 | Production of 2-Hydroxyacyl-CoAs and Derivatives Thereof - The production of substituted 2-hydroxyacyl-CoA molecules by a novel reaction is described. The reaction involves the condensation of formyl-CoA with a carbonyl-containing molecule. Such carbonyl-containing molecules include a substituted aldehyde and a ketone. The reaction is catalyzed by enzymes using a TPP-dependent mechanism. Also described is the production of unsubstituted and substituted 2-hydroxyacyl-CoA molecules comprising the condensation of formyl-CoA with a carbonyl-containing molecule, wherein the condensation is catalyzed by a prokaryotic HACL. The 2-hydroxyacyl-CoA can be converted to chemical products having broad applications by using enzyme catalysts. The combination of enzyme catalysts comprises novel biochemical reaction pathways that can be deployed either as polypeptides in a reaction buffer or genetically encoded in recombinant microorganisms. | 2022-05-26 |
20220162662 | CELL HYDROLYSATE COMPOSITION FROM CULTIVATED CELLS AND APPLICATIONS THEREOF - A cell hydrolysate composition, the composition comprising substantially all protein polypeptides and/or polypeptide fragments derived substantially from all the proteins in a cell from an in vitro cell culture. | 2022-05-26 |
20220162663 | LABEL-FREE DETECTION OF MYCOBACTERIA USING SURFACE ENHANCED RAMAN SPECTROSCOPY - A method of identifying a | 2022-05-26 |
20220162664 | INSTRUMENT AND SYSTEM FOR RAPID MICROORGANISM IDENTIFICATION AND ANTIMICROBIAL AGENT SUSCEPTIBILITY TESTING - A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing. | 2022-05-26 |
20220162665 | DIRECT DETECTION METHOD FOR TICK-BORNE INFECTIONS AND A CELL TECHNOLOGY MEDIUM FOR THE METHOD - This invention relates to a method for the direct detection of tick-borne infections, wherein the human/animal body fluid sample, ideally blood sample to be examined is obtained directly on a specified amount of cell technology medium establishing a partially hostile (semi-hostile) environment, the sample and the cell technology medium are mixed, incubated and stored, if required, meanwhile, the cells and pathogens of the subject are in the semi-hostile environment, then corpuscles and pathogens are separated; a small amount of blood cells from the “buffy coat” layer and blood cells located a few cell layers beneath it are supplemented to and mixed with the gained plasma, the sample is concentrated and the test is finally performed to detect pathogens in the plasma and in blood cells. | 2022-05-26 |
20220162666 | Soil Pathogen Testing - A method of detecting pathogens within a soil sample involves extracting DNA from two or more pathogens within the soil sample. The pathogens include soybean cyst nematodes and one or more specimens of | 2022-05-26 |
20220162667 | SYSTEMS FOR PROTEIN-PROTEIN INTERACTION SCREENING - Described herein are methods and systems useful for interrogating protein-protein interactions. These systems utilize non-functional fragments of polypeptides that can complement each other to form a fully functional fragment in vivo. The fully functional polypeptide releases a transcription regulating polypeptide that can bind and activate or repress a reporter element. | 2022-05-26 |
20220162668 | METHODS AND MATERIALS FOR ASSESSING RNA EXPRESSION - This document provides methods and materials for assessing RNA expression. For example, methods and materials for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), kits for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), and methods for making such kits are provided. | 2022-05-26 |
20220162669 | DNA-Antigen Exchange and Amplification - Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partners, (5) imaging the sample to detect bound labeled antigen-specific binding partners, (6) optionally removing/extinguishing signal from the optical labels, and (7) optionally repeating steps (1)-(6), or any subset thereof. | 2022-05-26 |
20220162670 | METHODS AND SYSTEMS TO IMPROVE THE SIGNAL TO NOISE RATIO OF DNA METHYLATION PARTITIONING ASSAYS - In an aspect, the present disclosure provides a method for determining a methylation status comprises: providing a biological sample of nucleic acid molecules; partitioning at least a subset of the nucleic acid molecules in the biological sample based on the methylation status of the nucleic acid molecules into a plurality of partitioned sets; digesting at least a subset of the one or more partitioned sets in the plurality of partitioned sets with at least one methylation sensitive restriction enzyme; enriching at least a subset of the nucleic acid molecules in the plurality of partitioned sets for genomic regions of interest, wherein the at least a subset of the nucleic acid molecules comprises digested nucleic acid molecules in the one or more partitioned sets; and determining methylation status at one or more genetic loci of the nucleic acid molecules in at least one of the partitioned sets. | 2022-05-26 |
20220162671 | METHODS AND SYSTEMS FOR BARCODING MULTIPLE NUCLEIC ACID ANALYTES - Methods, kit, systems, and compositions for processing nucleic acids and barcoding nucleic acids are disclosed. The methods and systems generally may comprise the presence of a support comprising nucleic acid barcode molecules which may be used to interact with nucleic acids to generate barcoded nucleic acid molecules. The support may have a plurality of nucleic acid barcode molecules that are able to barcode multiple analytes. This may allow the identification of multiple types of analytes and correlating the analytes as originating from a same biological particle. A splint and primer nucleic acids may also be used to generate barcoded nucleic acids. The methods and systems can be applied to a variety of biological samples and can analyze different nucleic acids, proteins, or other macromolecules of the biological samples. | 2022-05-26 |
20220162672 | METHODS FOR MULTIPLEX PCR - Methods for performing multiplex PCR-based enrichment of a target substrate are provided. Systems and methods for generating a sequencing library are also provided. | 2022-05-26 |
20220162673 | METHODS FOR PREPARATIVE IN VITRO CLONING - Methods and devices relate to the isolation of nucleic acids of interest from within a population of nucleic acids such as libraries of nucleic acid sequences. | 2022-05-26 |
20220162674 | HIGH THROUGHPUT OIL-EMULSION SYNTHESIS OF BOWTIE BARCODES FOR PAIRED MRNA CAPTURE AND SEQUENCING FROM INDIVIDUAL CELLS - Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. | 2022-05-26 |
20220162675 | METHODS AND KITS FOR THE ENRICHMENT AND DETECTION OF DNA AND RNA MODIFICATIONS AND FUNCTIONAL MOTIFS - Provided herein are methods for mapping modified nucleotide residues in nucleic acids. The methods include providing a nucleic acid sample in which non-target or target modified and unmodified nucleotide residues are converted to form of a different nucleotide (such a “C” being converted to “T”). Second strand synthesis is then performed on the converted nucleic acids using a set of anchored-base primers. Each primer in the set of anchored-base primers comprises one or more anchor bases at the 3′ terminus that are complementary to the target nucleotide (e.g., “G” or “CpG”), and a sequence of nucleotides selected from a set of sequences that could be a fully or partially degenerate set of sequences. For example, the sequence could be 5′-XnG-3′ and/or 5′-X(n−1)CG-3′, wherein X is any base, and n=2 to 25. Double-stranded nucleic acid products can be analyzed, for example by amplification and high throughput sequencing. | 2022-05-26 |
20220162676 | Methods and Kits for Detection of N-4-acetyldeoxycytidine in DNA - Provided herein is a new molecular marker in DNA: N-4-acetyldeoxycytidine (“N4-acdC”). Also provided herein are methods of detecting N4-acdC residues in DNA molecules as well as methods of using detected N4-acdC residues, for example in genetic mapping and diagnostics. | 2022-05-26 |
20220162677 | METHOD FOR CONDUCTING AN ASSAY - A method for performing a nucleic acid amplification assay employs a thermally-conductive receptacle holder with one or more receptacle wells, each conforming to an outer surface of a lower portion of a vial. A through-hole extends from an inner surface of each well to an outer surface of the holder. A thermal element is positioned proximal to the holder for altering a temperature of the holder. A signal detection module is configured to generate an excitation signal directed through the through-hole of the well and detect an optical emission from a fluid contained in the vial supported by the well. At least one well supports a capped vial containing a reagent for a nucleic acid amplification assay and including an opaque cap sealing an open end of the vial. The lower portion of the vial is contained within a well, and the cap is situated above a top surface of the holder. | 2022-05-26 |
20220162678 | METHOD FOR DETERMINING WHETHER ORGANISM HAVING CELL WALL EXISTS AND METHOD FOR IDENTIFYING ORGANISM HAVING CELL WALL - A method of determining presence state of an organism having cell wall includes: preparing a liquid sample by immersing a measurement sample in water solvent, or preparing a measurement sample that is water solvent as a liquid sample; and detecting presence state of one or more sRNA species in the liquid sample, wherein presence state of an organism having cell wall is determined based on the presence state of the one or more sRNA species. A method of identifying an organism having cell wall includes: preparing a liquid sample by immersing a measurement sample in water solvent, or preparing a measurement sample that is water solvent as a liquid sample; and detecting presence state of one or more sRNA species in the liquid sample, wherein an organism having cell wall present in the measurement sample is identified based on the presence state of the one or more sRNA species. | 2022-05-26 |
20220162679 | CLOSED NUCLEIC ACID STRUCTURES - The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications. | 2022-05-26 |
20220162680 | COMPOSITIONS AND METHODS FOR SEQUENCING USING FLUOROPHORES AND QUENCHERS OR DONORS - A method is provided that includes adding, using a polymerase coupled to a substrate, nucleotides in a solution to a first polynucleotide using at least a sequence of a second polynucleotide. The method includes separating, using labels respectively coupled to the nucleotides, quenchers from respective fluorophores. The method includes then detecting a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least fluorescence from the respective fluorophores. | 2022-05-26 |
20220162681 | NOVEL PNA OLIGOMER, USE THEREOF FOR DETECTING DNA METHYLATION, AND METHOD FOR DETECTING DNA METHYLATION USING SAME - The present invention relates to a modified PNA oligomer for detecting gene methylation. By using a PNA probe modified by introducing a methyl group-specific substituent to the gamma position, N-terminus or C-terminus of the PNA, the present invention may be used for a method for detecting using a difference in physical properties between a gene and a non-methylated gene caused by an interaction between the probe and methyl groups of the gene. | 2022-05-26 |
20220162682 | ASSESSMENT OF MUTATION BURDEN IN SKIN - Disclosed herein is a method of quantifying mutation burden of skin based on genomic mutations. In some instances, also described herein are methods of reducing skin cancer risk, such as that caused by UV damage or other environmental factor. | 2022-05-26 |
20220162683 | COMPOSITIONS AND METHODS FOR PERFORMING HYBRIDIZATIONS WITH NO DENATURATION - The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences. | 2022-05-26 |
20220162684 | AFFINITY REAGENTS HAVING ENHANCED BINDING AND DETECTION CHARACTERISTICS - An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component. | 2022-05-26 |
20220162685 | METHOD FOR ANALYZING CELL SAMPLE HETEROGENEITY - The present invention relates to a method for analyzing a biological sample on a single cell level by compartmentalizing said sample using a grid and performing an optimized combinatorial indexing protocol within cells of the compartmentalized tissue while deducing cell-specific information regarding the cell identity and activity in the spatial context within the sample. | 2022-05-26 |
20220162686 | MEMBRANE-BASED, IN-GEL LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) SYSTEM AND METHOD FOR DETECTING MICROBES - Disclosed herein is a membrane-based, in-gel loop-mediated isothermal amplification (LAMP) system, kit and method for detection of a target microorganism in a sample suspected of containing the microorganism. LAMP reagents, a lysing agent and a hydrogel are placed together on a filter membrane loaded with a pre-filtered sample. The hydrogel polymerizes over a short time to immobilize any target DNA/RNA particles on the membrane. The system may include a compact, portable device that integrates heat incubation and fluorescence illumination, and also a cloud-based smartphone image analysis application for quantitative results interpretation. If target DNA/RNA are present in the sample, fluorescent amplicons are produced as a result of LAMP reaction. The target microorganisms are detected by visually detecting the presence or absence of the amplicons. The method may be employed for rapid and inexpensive point-of-use (POU) absolute quantification of SARS-CoV-2 in environmental water or wastewater samples with high sensitivity. | 2022-05-26 |
20220162687 | NOVEL SYSTEMS, METHODS AND COMPOSITIONS FOR THE DIRECT SYNTHESIS OF STICKY ENDED POLYNUCLEOTIDES - The current inventive technology includes systems, methods, and compositions for directly synthesizing sticky ended DNA fragments for subsequent gene assembly. In a preferred embodiment, the inventive technology includes strategies for the direct synthesis of sticky ended DNA with 5′ overhangs that have any desired length and base composition, using typical PCR protocols with no additional manipulation. In another embodiment, the inventive technology includes the direct synthesis of sticky ended DNA using chemically modified oligonucleotide primers in a polymerase chain reaction (PCR). In certain embodiments, the inventive technology allows for the generation of larger DNA constructs formed by the sticky-ended assemblies generally described herein compared to traditional synthesis and ligation applications. | 2022-05-26 |
20220162688 | Enhanced Adaptor Ligation - The present disclosure describes a method of adaptor ligation to the ends of the fragmented double-stranded DNA molecules. | 2022-05-26 |
20220162689 | METHOD AND DEVICE FOR SEQUENCING AND DETECTING DNA/RNA DIRECTLY ON A SENSOR - The present invention is related to DNA/RNA sequencing and in particular approaches termed Next Generation Sequencing (NGS). Improved methods and integrated devices are disclosed, which enable faster and more efficient analysis of nucleic acids. One aspect of the invention has to do with integration of detection system into consumable flowcell. This enables miniaturization and simplification of sequencing instrumentation while simultaneously reducing sequencing costs. By performing sequencing and detection directly on the sensor, such as CCD or CMOS, one can significantly speed up the sequencing process. | 2022-05-26 |
20220162690 | METHOD AND SYSTEM FOR SEQUENCING NUCLEIC ACIDS - Provided are compositions, methods and systems for determining the sequence of a template nucleic acid using a polymerase-based, sequencing-by-binding procedure. An examination step involves monitoring the interaction between a polymerase and template nucleic acid in the presence of one or more nucleotides. Identity of the next correct nucleotide in the sequence is determined without incorporation of any nucleotide into the structure of the primer by formation of a phosphodiester bond. An optional incorporation step can be used after the examination step to extend the primer by one or more nucleotides, thereby incrementing the template nucleotides that can be examined in a subsequent examination step. The sequencing-by-binding procedure does not require the use of labeled nucleotides or polymerases, but optionally can employ these reagents. | 2022-05-26 |
20220162691 | METHOD FOR PREPARATION AND HIGH- THROUGHPUT MICROBIAL SINGLE-CELL RNA SEQUENCING OF BACTERIA - Methods and kits for uniquely labeling nucleic acid molecules within a plurality of microbial cells are described. In an embodiment, the method comprises fixing and permeabilizing the plurality of microbial cells; dissociating microbial cell aggregates within a suspension comprising the plurality of microbial cells; reverse transcribing mRNA within the plurality of microbial cells to provide cDNA; and combinatorially labelling the cDNA to provide labelled cDNA. | 2022-05-26 |
20220162692 | Assemblies - The invention provides an assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages, when bonded to the scaffold, are capable of interacting to form a pore in a layer of amphipathic molecules. | 2022-05-26 |
20220162693 | MASSIVELY PARALLEL SEQUENCING USING UNLABELED NUCLEOTIDES - The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent. | 2022-05-26 |
20220162694 | DNA ARRAY - Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. | 2022-05-26 |
20220162695 | USE OF DEXTRAMER IN SINGLE CELL ANALYSIS - Disclosed herein include systems, methods, compositions, and kits suitable for the use of dextramers in single cell analysis. In some embodiments, one or more primers allowing generation of separate libraries for proteins (e.g., antibodies) and for dextramers are used. | 2022-05-26 |
20220162696 | METHODS, DEVICES, AND SYSTEMS FOR ANALYTE DETECTION AND ANALYSIS - Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles. | 2022-05-26 |
20220162697 | METHODS FOR DETERMINING KINETIC PARAMETERS OF A REACTION BETWEEN ANALYTE AND LIGANDS - According to the present invention there is provided a method for determining kinetic parameters of a reaction between analyte and ligands, the method comprising the steps of, a) flowing a first volume of sample fluid (V | 2022-05-26 |