22nd week of 2010 patent applcation highlights part 46 |
Patent application number | Title | Published |
20100136585 | METHOD AND DEVICE FOR THE DETERMINATION OF MINOR CELL POPULATION IN HETEROGENEOUS CELL POPULATIONS - The invention relates to a method for determining one or more cellularly bound analytes in a liquid sample, said method being carried out using a device comprising: at least one feeding zone ( | 2010-06-03 |
20100136586 | METHOD FOR THE SURVEILLANCE FOR BIOLOGICAL, CHEMICAL AND RADIOLOGICAL AGENTS - Provided is a method of surveillance for hazardous materials, e.g., chemical, biological and radiological agents. The method comprises assaying a sample derived from materials collected from a sample domain for the presence of a chemical, biological, or radiological agent. The sample domain comprises at least one collection point from which the materials are collected in a pre-existing operation unrelated to surveillance. | 2010-06-03 |
20100136587 | Method of Identifying Risk for Thyroid Disorder - A method for identifying a patient that is at risk for developing a thyroid disorder that occurs subsequent to treatment with a regimen that depletes lymphocytes, comprising determining whether antibodies directed against thyroid peroxidase or thyroid microsomes are present in the patient, wherein if the antibodies are present in the patient then the patient is at increased risk for developing a thyroid disorder. A particular embodiment is a method for identifying a patient with multiple sclerosis that is at risk for developing a thyroid disorder that occurs subsequent to treatment with a regimen that depletes CD52-positive cells, comprising determining whether antibodies directed against thyroid peroxidase or thyroid microsomes are present in the patient, wherein if the antibodies are present in the patient then the patient is at risk for developing the thyroid disorder. | 2010-06-03 |
20100136588 | Methods and Devices for Diagnosis of Appendicitis - A method is provided for determining the severity of appendicitis in a patient that includes testing a blood, serum or plasma sample from the patient for the quantity of MRP8/14 in the sample and comparing it with the quantity of MRP8/14 present in standard samples correlated with an appendicitis severity scoring system. A histologically-based appendicitis severity scoring system is also provided. Immunoassays and kits for performing the appendicitis assays of this invention are also provided, as are standard samples and data correlating MRP8/14 quantities present in patient samples to histologically-based appendicitis severity grades. The methods and immunoassay devices and kits of this invention are useful for managing the treatment of patients presenting with appendicitis symptoms. | 2010-06-03 |
20100136589 | Methods and Kits for Determining von Willebrand Factor Activity in the Absence of Ristocetin and for Determining the Activity of ADAMTS-13 Protease - Described herein are method(s), kit(s), reagent(s) and the like for determining von Willebrand factor (VWF) activity in a sample in the absence of ristocetin. | 2010-06-03 |
20100136590 | Method of Determining NT-proBNP - The invention relates to a method of determining equine NT-proBNP, or fragments thereof, comprising the steps of: providing an equine sample, contacting the sample with at least one antibody which specifically binds to equine NT-proBNP, and determining the presence and/or concentration of the equine NT-proBNP or fragments thereof existing in the sample. | 2010-06-03 |
20100136591 | METHOD OF MEASURING LIPID IN SPECIFIC LIPOPROTEIN - A method of measuring a lipid in a specific lipoprotein characterized by using a polycyclic polyoxyalkylene derivative at least in the step of determining the specificity of the measurement of the target lipid. | 2010-06-03 |
20100136592 | Photo-Induced Damage Mitigating Agents and Preparation and Methods of Use Thereof - Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period. | 2010-06-03 |
20100136593 | METHOD FOR DIAGNOSING AND DISTINGUISHING STROKE AND DIAGNOSTIC DEVICES FOR USE THEREIN - A method for determining whether a subject has had a stroke and, if so, the type of stroke which includes analyzing the subject's body fluid for at least four selected markers of stroke, namely, myelin basic protein, S100 protein, neuronal specific enolase and a brain endothelial membrane protein such as thrombomodulin or a similar molecule. The data obtained from the analyses provide information as to the type of stroke, the onset of occurrence and the extent of brain damage and allow a physician to determine quickly the type of treatment required by the subject. | 2010-06-03 |
20100136594 | HIGH-THROUGHPUT ASSAY FOR EVALUATING DIPEPTIDYL PEPTIDASE I ACTIVITY - A method of screening a compound which modulates dipeptidyl peptidase I (DPPI) activities comprises the steps of adding a peptide substrate of DPPI to a reaction mixture which comprises DPPI and a compound, wherein the peptide substrate of DPPI has at least 3 amino acids and binds to a binding site of DPPI in addition to the S | 2010-06-03 |
20100136595 | HYDROCARBON-FORMING OXIDATIVE DECARBONYLASE ENZYME, HYDROCARBONS PRODUCED THEREBY, AND METHOD OF USE - The present disclosure relates to oxidative decarbonylase enzymes, methods of making hydrocarbons with such enzymes, hydrocarbons produced therefrom and uses thereof. More particularly, the present disclosure relates to isolated polypeptide sequences that are cytochrome P450 enzymes with oxidative decarbonylase activity and methods of their use to generate hydrocarbon products, such as biofuels. | 2010-06-03 |
20100136596 | DEVICE FOR RECEIVING, TREATING, AND STORING SMALL VOLUME SAMPLES - A device is provided by means of which biomolecules in samples in a volume range from <1 μl to 500 μl can be received, treated and stored in a quick, reproducible and loss-free manner as easily, effortlessly and practically as possible, and without any risk of damaging the device. The device includes sample vessels that are configured, in terms of size and shape, as dimensionally and positionally stable capillaries that are open at both ends, the longitudinal walls of which are completely or partially made of a semi-permeable membrane. | 2010-06-03 |
20100136597 | METHOD FOR MODULARTING ACTIVITY OF T LYMPHOCYTES - The invention relates to methods and compositions which modulate T lymphocyte activity. It has been found that two, T lymphocyte receptors, especially TCR and CD8, are present at a distance from each other on T lymphocyte surfaces. Via use of modulators which change the distance between these receptors, the activity of the T lymphocyte is modulated. | 2010-06-03 |
20100136598 | NOVEL MESENCHYMAL PROGENITOR CELLS DERIVED FROM HUMAN BLASTOCYST-DERIVED STEM CELLS - Described herein is a mesenchymal human progenitor (hBS-MP) cell population derived from human blastocyst-derived stem (hBS) cells and a method to obtain the progenitor cell population in which is eliminated the need of co-culture steps, cell sorting, manual selection, and transfections. Also, the use of the hBS-MP cells in drug discovery and specifically for toxicity testings as well as for therapeutic use is made possible. | 2010-06-03 |
20100136599 | METHODS AND PRODUCTS RELATED TO THE IMPROVED ANALYSIS OF CARBOHYDRATES - The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information regarding the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation on protein function. Such methods are also provided. Methods are also provided to assess protein production processes, to assess the purity of proteins produced, and to select proteins with the desired glycosylation. | 2010-06-03 |
20100136600 | URINE BASED DETECTION OF A DISEASE STATE CAUSED BY A PNEUMOCOCCAL INFECTION - There is provided a method of diagnosing a disease state in a subject. The disease state is caused or effected by pneumococcal infection. The method includes obtaining a biological test sample from the subject. The biological sample includes at least one metabolite selected from the group consisting of citrate, trigonelline, acetylcarnitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine. A respective concentration of each one of the at least one metabolite is compared with a predetermined concentration value associated with a corresponding one of the at least one metabolite. The predetermined concentration value is indicative of the disease state. For example, the biological sample includes any one of citrate, trigonelline, acetylcamitine, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine. As a further example, the biological samples include any combination of citrate, trigonelline, acetylcamitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine. As a further example, the biological sample includes each one of citrate, trigonelline, acetylcamitine, acetone, myoinositol, 3-hydroxybutyrate, glucose and carnitine. | 2010-06-03 |
20100136601 | GLASS-CELL VIAL FOR EXAMINING SLIGHT AMOUNT OF SPECIMEN - A glass-cell vial is provided which can examine specimen without causing any hindrance even when as minimum as possible specimen is used, and prevents mixture of foreign substances from causing examination errors. | 2010-06-03 |
20100136602 | SOUR TASTE RECEPTOR COMPOSITIONS AND METHODS - The present invention relates to sour taste receptors and compositions and methods thereof. In particular, the present invention provides assays and methods of screening for ligands specific for sour taste receptors. Additionally, the present invention provides methods for screening for accessory proteins and mutations, polymorphisms and other potential sour taste receptor protein mutations that are associated with disease states, and therapeutic agents, ligands, and modulators of such proteins. The present invention also provides compositions and methods for modulating sour taste receptors in vitro and in vivo. | 2010-06-03 |
20100136603 | KIT AND A METHOD FOR EVALUATING TOXICITY USING SPORE RELEASE BY THE GREEN ALGA ULVA - The present invention relates to a kit and a method for evaluating toxicity of a waterbody sample by a spore release of a green alga | 2010-06-03 |
20100136604 | IN VITRO TUMOR ANGIOGENESIS MODEL - Provided is a method of inducing tubulogenesis in normal endothelial cells comprising co-culturing the normal endothelial cells with tumor cells and forming tubules from the normal endothelial cells. | 2010-06-03 |
20100136605 | SYSTEM FOR AND METHOD OF POSITIONING CELLS AND DETERMINING CELLULAR ACTIVITY THEREOF - A device for positioning at least one cell in at least one addressable position, the device comprising a substrate formed with at least one addressable pore and at least one channel embedded in the substrate and being in fluid communication with the at least one pore. The at least one pore and the at least one channel are designed and constructed such that an under-pressure formed in the at least one channel results in vacuum adherence of the at least one cell onto the at least one pore, such that a single cell is vacuum adhered onto a single pore. In one embodiment, the substrate is a non-conductive substrate and is further formed with one or more electrode structures, where each of the electrode structures is positioned in one of the pores. In an additional embodiment the device is designed locatable onto an organ, such as a brain. | 2010-06-03 |
20100136606 | METHOD OF MEASURING PHYSICAL PROPERTY VALUES OF CELL AND PHYSICAL PROPERTY MEASURING APPARATUS - Firstly, a shape of a cell as an object of a measurement is modeled, and a complex electric permittivity response when an AC electric field is applied to the cell is obtained through a numerical analysis (Step S | 2010-06-03 |
20100136607 | SENSOR DEVICE - The invention relates to a sensor device for detecting CO | 2010-06-03 |
20100136608 | Multiple Filter Array Assay - The present invention relates to a method and device for the assay and determination of the concentration of organisms or other filterable constituents of a fluid test sample. The method includes the steps of exposing, processing, and analyzing a liquefied sample in a filter tray apparatus. Each filter element (FE) filters a volume of liquid from a common fluid test sample. Excess unfiltered liquid and filtrate is removed from the filter tray apparatus. Each filter element contained in the filter tray apparatus is exposed to culture medium and/or appropriate testing reagents introduced to the apparatus to generate an indicator of the filterable constituent. Final examination is conducted on the filter tray apparatus for the indication of the presence and/or absence of filtered constituents in the multiple filter elements. The presence/absence information defines the concentration of the filterable constituent in the fluid test sample. | 2010-06-03 |
20100136609 | Method for separation, characterization and/or identification of microorganisms using raman spectroscopy - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements. | 2010-06-03 |
20100136610 | Methods And Microfluidic Devices For Single Cell Detection Of Escherichia Coli - The present invention features a microfluidic device for detecting | 2010-06-03 |
20100136611 | METHOD OF DETECTING BACTERIAL CONTAMINATION USING DYNAMIC LIGHT SCATTERING - Methods of detecting bacterial contamination in a platelet concentrate are performed using a dynamic light scattering (DLS) instrument and a sample holder. A sample of platelet concentrate can be held vertically or horizontally in a capillary in the sample holder. Alternatively, novel platelet storage bags modified to include an optically translucent window can be held within another variant of the sample holder. Still alternatively, platelet storage bags having one or more tubes detachably appended to the bag can be used. A sample is drawn off into an appended tube for placement directly into the sample holder. This method provides a number of related, non-invasive techniques for detecting whether bacteria has contaminated a platelet concentrate. Contamination indicators include a population of particles different from platelets, microparticles or proteins, bad-quality platelets, i.e. low DLS score, and very high or very low scattering intensity. | 2010-06-03 |
20100136612 | HORIZONTAL ANTIGEN RETRIEVAL - The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation. | 2010-06-03 |
20100136613 | PRESSURE-ASSISTED MOLECULAR RECOVERY (PAMR) OF BIOMOLECULES, PRESSURE-ASSISTED ANTIGEN RETRIEVAL (PAAR), AND PRESSURE-ASSISTED TISSUE HISTOLOGY (PATH) - A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times. | 2010-06-03 |
20100136614 | Dendrimer-like modular delivery vector - Various nucleic acid-based matrixes are provided, comprising nucleic acid monomers as building blocks, as well as nucleic acids encoding proteins, so as to produce novel biomaterials. The nucleic acids are used to form dendrimers that are useful as supports, vectors, carriers or delivery vehicles for a variety of compounds in biomedical and biotechnological applications. In particular, the macromolecules may be used for the delivery of drugs, genetic material, imaging components or other functional molecule to which they can be conjugated. An additional feature of the macromolecules is their ability to be targeted for certain organs, tumors, or types of tissues. Methods of utilizing such biomaterials include delivery of functional molecules to cells. | 2010-06-03 |
20100136615 | METHOD OF PRODUCING SOYBEAN PROTEIN MATERIAL - It is intended to provide a soybean protein material which is highly dispersible in hot water and highly miscible with water so as to give a solution showing regulated coarseness. More specifically, it is intended to provide a soybean protein material which is appropriately usable in preparing protein drinks. According to a method comprising the following steps, it becomes possible to produce a soybean protein material which is dispersible in hot water and miscible with water: the step wherein soybean protein is hydrolyzed with a protease in an aqueous system until the 0.22 MTCA solubility attains 15 to 30%; the step wherein an emulsifier having an HLB value of 6 or more but not more than 13 is added to the soybean protein; the step of drying the soybean protein after the hydrolysis; and the step of heating the soybean protein in an aqueous system at 105 to 180° C. for 15 to 180 seconds before the drying. | 2010-06-03 |
20100136616 | Selection of Host Cells Expressing Protein at High Levels - The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in said multicistronic transcription unit, and wherein an internal ribosome entry site (IRES) is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG startcodon. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, said host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention. | 2010-06-03 |
20100136617 | HALOHYDRIN EPOXIDASE - The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II. | 2010-06-03 |
20100136618 | METHOD FOR PRODUCING CELLULASE AND HEMICELLULASE HAVING HIGH HYDROLYTIC ACTIVITY - The present invention provides a novel cellulase-producing fungus, i.e. | 2010-06-03 |
20100136619 | Expression System - A protein expression system is provided. The system comprises: a) a T7 RNA polymerase-dependent promoter operably linked to an expression cassette for a protein of interest; and b) an expression cassette for T7 RNA polymerase operably linked to a λpL promoter and at least two perfect palindrome operator sequences. | 2010-06-03 |
20100136620 | SOLUBLE, STABLE FORM OF HDM2, CRYSTALLINE FORMS THEREOF AND METHODS OF USE THEREOF - The present invention discloses modified Hdm2 proteins that are soluble. In addition, the present invention discloses nucleic acids that encode the modified Hdm2 proteins of the present invention. The invention also provides crystals of modified Hdm2 proteins that are suitable for X-ray crystallization analysis. The present invention also discloses methods of using the modified Hdm2 proteins and crystals thereof to identify, select and/or design compounds that may be used as anticancer agents. The present invention further discloses compounds that bind to modified Hdm2 proteins in protein-ligand complexes. | 2010-06-03 |
20100136621 | SOLUBILITY TAGS FOR THE EXPRESSION AND PURIFICATION OF BIOACTIVE PEPTIDES - Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag. | 2010-06-03 |
20100136622 | Method for the Production of Proteins - The present invention relates to a process for the purification of a protease. | 2010-06-03 |
20100136623 | MUTATIONS IN ION CHANNELS - A method of identifying a subject predisposed to a disorder associated with ion channel dysfunction, comprising ascertaining whether at least one of the genes encoding ion channel subunits in said subject has undergone a mutation event such that a cDNA derived from said subject has the sequence set forth in one of SEQ ID NOS: 1-134. | 2010-06-03 |
20100136624 | Method for making monoclonal antibodies and cross-reactive antibodies obtainable by the method - A method of making monoclonal antibodies according to a mixed antigen immunization protocol is described. In addition, antibodies obtainable by the method are disclosed which specifically cross-react with two or more different receptors to which Apo-2 ligand (Apo-2L) can bind. | 2010-06-03 |
20100136625 | METHOD FOR THE HIGH LEVEL EXPRESSION OF ACTIVE LYMPHOTOXIN-BETA RECEPTOR IMMUNOGLOBULIN CHIMERIC PROTEINS AND THEIR PURIFICATION - Methods for high level expression of active lymphotoxin-β receptor immunoglobulin chimeric proteins and their purification. | 2010-06-03 |
20100136626 | Production and Use of Novel Peptide-Based Agents for Use with Bi-Specific Antibodies - The present invention relates to a bi-specific antibody or antibody fragment having at least one arm that is reactive against a targeted tissue and at least one other arm that is reactive against a targetable conjugate. The targetable conjugate encompasses a hapten to which antibodies have been prepared. In preferred embodiments, the hapten is histamine-succinyl-glycine (HSG). In more preferred embodiments, the at least one arm comprises the CDR sequences of the HSG-binding 679 antibody. The targetable conjugate is attached to one or more therapeutic and/or diagnostic agents. The invention provides constructs and methods for producing the bispecific antibodies or antibody fragments, as well as methods for using them and kits comprising them. | 2010-06-03 |
20100136627 | Engineered Rabbit Antibody Variable Domains and Uses Thereof - The present invention relates to methods for screening and producing polypeptides that immunospecifically bind to an antigen, which: polypeptides comprise binding domains that are derived from rabbit immunoglobulin. Using rabbit antibody heavy-chain or light-chain scaffolds, the methods of the invention allow identification of novel CDR loops and framework regions that confer enhanced stability and/or affinity to isolated immunoglobulin variable domains, in particular, relative to those derived from rodent antibodies. The enhanced stability and/or affinity of the variable domains of the invention permit their use in the production research tools or therapeutic immunospecific polypeptides, including single domain immunospecific polypeptides, i.e., comprising one of a V | 2010-06-03 |
20100136628 | Novel OX40R Binding Agents - The present invention discloses peptides isolated from the extracellular domain of OX40 Ligand (OX40L) capable of binding OX40 Receptor (OX40R) and inhibiting OX40R-OX40L interaction. Such peptides, fusion proteins comprising them, as well as peptides and other molecules designed on their sequences, can be used as OX40R binding agents competing with natural OX40L for blocking OX40R-mediated cell signaling in the prophylaxis and/or treatment of diseases related to activated T cells. | 2010-06-03 |
20100136629 | ENHANCED ETHANOL FERMENTATION USING BIODIGESTATE - Methods and systems for enhancing ethanol production using a suspending fluid are described. The suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms. Also described are methods and systems for hydrolyzing a feedstock for fermentation that include hydrolyzing a feedstock suspension. The feedstock suspension can include feedstock that includes complex sugars, and a suspending fluid, wherein the suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms. | 2010-06-03 |
20100136630 | PRODUCTION OF LOW MOLECULAR WEIGHT HYALURONIC ACID - The present invention relates to methods for producing a hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton, the methods comprising the steps of:
| 2010-06-03 |
20100136631 | IMPROVED PRIMERS AND PROBES FOR THE AMPLIFICATION AND DETECTION OF HIV GAG,REV AND NEF - The invention relates to improved methods and compositions for the nucleic acid amplification of one or multiple variants (strains) of Human Immunodeficiency Virus (HIV) present in a sample, and preferably in a sample from a pathogen infected individual. In particular, novel primers, methods and kits for the amplification of one or more species of HIV Rev, Gag and Nef nucleic acids are provided. The amplified HIV nucleic acid can be used to identify and/or quantitate HIV variants present in a sample. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can also be used directly as vaccines or to transfect/load antigen presenting cells. The loaded antigen presenting cells can be used as a vaccine for the treatment or prevention of HIV infection. | 2010-06-03 |
20100136632 | FULLY AUTOMATED PORTABLE DNA DETECTION SYSTEM - Provided herein is a portable thermocycler, comprising: (i) a case; (ii) a rotary plate in the case; (iii) a plurality of heating blocks arranged in a geometric pattern disposed on the rotary plate; and (iv) at least one vessel adapted to move and contact at least two of the plurality of heating blocks; wherein each of the heating blocks comprises a heating plate maintained at a set temperature over a thermally insulating material; wherein the geometric pattern comprises a number of center heating blocks arranged in a shape defining a polygon and a number of outside heating blocks disposed around the periphery of the rotary plate; and wherein the rotary plate includes a plurality of rotating wheels adapted to rotate at least one of the vessels into contact with each of the heating blocks. | 2010-06-03 |
20100136633 | COMPOSITIONS AND METHODS FOR THE ASSEMBLY OF POLYNUCLEOTIDES - The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with promer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides. | 2010-06-03 |
20100136634 | METHOD AND EQUIPMENT FOR PRODUCTION OF GLUCOSE, ETHANOL,FURFURAL,FURANE AND LIGNIN FROM RENEWABLE RAW MATERIALS - Method and equipment for production of fermentable saccharides, ethanol, furfural, furane, lignin, acetic acid and formic acid from lignocellulosic and amylaceous materials. The method comprises one-stage or two-stage continuous thermo-compressive hydrolysis of ligno-cellulosic particles, cellulase treatment of unreacted lignocellulose, amylase treatment of formed monosaccharides combined with added amylaceous materials, and fermentation of the combined processed monosaccharide solutions into ethanol. Side products furfural, methanol, acetic acid, formic acid and lignin are recovered and purified, optionally furfural is further converted to furan. An integrated process for recovery and recycling of all products and by-products, and recycling of heat energy is disclosed. | 2010-06-03 |
20100136635 | Method of Inducing Chirality to Epoxides Using 2,3:4,6 di-O-isopropylidene-2-keto-L-gulonic Acid Monohydrate - The present invention relates to a recyclable method to prepare chirally pure epoxides directly from olefins using a novel chiral acid 2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid monohydrate as chiral inducer and anhydrous hydrogen peroxide in the form of urea hydrogen peroxide adduct (UHP) as oxidant and lipase enzyme as catalyst. Under the influence of lipase, the chiral acid 2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid monohydrate is converted to per-acid in situ by the oxidant urea hydrogen peroxide which in turn transfers oxygen to olefin inducing chirality to the product epoxide. Using this technique, important epoxide building blocks of different industrially important compounds can be prepared with good yield and moderate enantioselectivity. Chiral epoxidation of olefins with environment friendly catalytic system that can be recycled is highly desirable in the context of Green Chemistry for preparation of many industrially important compounds. | 2010-06-03 |
20100136636 | PROCESS FOR SELECTIVE PRODUCTION OF THEAFLAVIN - The present invention provides a method for selective production of theaflavin in large amounts at high yield, and in an easy and inexpensive manner. Specifically, it relates to a method for selective production of theaflavin whereby a processed plant extract containing epicatechin, epigallocatechin, epicatechin-3-O-gallate and epigallocatechin-3-O-gallate is combined with a plant cell culture having peroxidase activity for selective production of theaflavin. | 2010-06-03 |
20100136637 | RECOMBINANT MICROORGANISM HAVING A PRODUCING ABILITY OF POLYLACTATE OR ITS COPOLYMERS AND METHOD FOR PREPARING POLYACTATE OR ITS COPOLYMERS USING THE SAME - Provided are a recombinant microorganism capable of producing polylactate (PLA) or hydroxyalkanoate-lactate copolymers and a method of preparing PLA or hydroxyalkanoate-lactate copolymers using the same. The recombinant microorganism has both a gene encoding a propionyl-CoA transferase from | 2010-06-03 |
20100136638 | PRODUCTION OF 3-HYDROXYPROPIONIC ACID USING BETA-ALANINE/PYRUVATE AMINOTRANSFERASE - Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed. | 2010-06-03 |
20100136639 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 2010-06-03 |
20100136640 | ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME - The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified. | 2010-06-03 |
20100136641 | STRAIN FOR BUTANOL PRODUCTION WITH INCREASED MEMBRANE UNSATURATED TRANS FATTY ACIDS - Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase. | 2010-06-03 |
20100136642 | RECOVERY OF LIGNIN AND WATER SOLUBLE SUGARS FROM PLANT MATERIALS - In one aspect, a process for treating woody plant material is provided, the process involving contacting the plant material with a continuous flow of an aqueous ethanol solution at elevated temperature and pressure under conditions that promote extraction of ethanol-soluble lignin from the plant material and retention of hemicellulose sugars, xylose and cellulose in the treated plant material solids. In another aspect, a process for extracting hemicellulose sugars from lignin-depleted plant material solids is provided, the process involving contacting lignin-depleted plant material with water at elevated temperature and pressure under conditions that promote extraction of hemicellulose sugars from the plant material; and recovering hemicellulose sugars from the liquid mixture. | 2010-06-03 |
20100136643 | METHOD AND APPARATUS FOR PRODUCING HYDROGEN AND MICROORGANISM-IMMOBILIZATION PELLETS USED IN THE SAME - The hydrogen-producing method of the an aspect of the present invention is a method for producing hydrogen in which hydrogen is produced from an organic matter using a microorganism, characterized by using pellets on which hydrogen-producing acid-resistant bacteria are entrapped and immobilized, producing hydrogen by bringing the pellets into contact and react with the organic matter in an environment of a pH of 4 to 6. The inventors of the present invention have obtained a finding that a hydrogen-producing bacteria are entrapped and immobilized, so that the optimum pH of the hydrogen-producing bacterium shifts to low range. The present invention is made based on the above finding and uses pellets on which the hydrogen-producing bacteria are entrapped and immobilized, so that the hydrogen-producing bacterium is activated at a low pH range of 4 to 6 to produce hydrogen. Therefore, the effects of contaminated bacteria that consume hydrogen are less, and the yield of hydrogen can be improved. | 2010-06-03 |
20100136644 | METHODS FOR IMPROVING THE CULTIVATION OF AQUATIC ORGANISMS - The present invention relates to electrochemical methods for altering a biological characteristic of an aquatic organism such as an algae or bacterium. The invention includes alterations to biological characteristics such as growth rate, membrane permeability and buoyancy. | 2010-06-03 |
20100136645 | METHOD FOR PREPARING A CELL-DERIVED EXTRACELLULAR MATRIX SCAFFOLD - The present invention relates to a method for fabricating a cell-derived extracellular matrix scaffold, more particularly, to a method for fabricating a cell-derived extracellular matrix scaffold, the method comprising the steps of obtaining a chondrocyte/extracellular matrix (ECM) membrane from chondrocytes derived from animal cartilage, obtaining a pellet-type scaffold-free construct by culturing after centrifuging the obtained chondrocytes/extracellular matrix (ECM) membrane and freeze-drying the obtained pellet-type construct. The cell-derived ECM scaffold according to the invention is a porous scaffold fabricated using cartilage tissue engineered by culturing chondrocytes in an in vitro scaffold-free system, which is not reduced in size during the cultivation and thus useful for cartilage regeneration. | 2010-06-03 |
20100136646 | Selective Inactivation Of Microorganisms With A Femtosecond Laser - A method is provided for selectively inactivating microorganisms with femtosecond pulsed lasers. Under proper laser conditions, irradiation of the femtosecond pulsed laser causes inactivation of pathogenic microorganisms, for example, viruses, bacteria and protozoa, without causing cytotoxicity in mammalian cells. Pathogenic microorganism activity is diminished through an impulsive stimulated Raman scattering process, that is, through the excitation of the low-energy vibrational state on the outer structure of a microorganism with femtosecond pulsed lasers. The wavelength of the laser pulses is in a range of the electromagnetic spectrum, for example, visible and near-infrared where water is substantially transparent. The method is utilized for cleansing blood components, disinfecting drinking water, treating viral and bacterial diseases, extracting nucleic acid from microorganisms, and for manufacturing vaccines. | 2010-06-03 |
20100136647 | METHOD FOR PRODUCTION OF CELL ATTACHMENT AND CULTURE SURFACES - The present invention relates to the field of adherent cell culture. More closely, the invention relates to a method for production of a cell attachment and culture surface, such as a microcarrier, comprising a guanidino-containing ligand, wherein the ligand is coupled via reaction involving a primary amine to the surface which is activated by activation groups such that the final molar ratio of grafted ligand and ungrafted activation groups is above 1.5. Preferably, the ligand density is above 0.5 mmol/g cell culture surface and the remaining activation groups after coupling is less than 0.6 mmol/g cell culture surface. The cell culture surface may be used for various purposes, primarily cell cultivation and virus production. | 2010-06-03 |
20100136648 | Expansion Moulding of Shape Memory Polymers - The present invention relates to shape memory materials and to a method for controlling shape change in shape memory materials. In particular, the invention relates to a method and a system for forming complex shapes from shape memory materials and to shape memory materials having complex shapes. | 2010-06-03 |
20100136649 | Encapsulation of Cells in Biologic Compatible Scaffolds by Coacervation of Charged Polymers - This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold. | 2010-06-03 |
20100136650 | MECHANISM-BASED INACTIVATORS OF AUTOTAXIN - A novel class of compounds to inactivate autotaxin enzymes is provided. Such compounds include mono- and di-fluoromethylphenyl C | 2010-06-03 |
20100136651 | DETECTION, ISOLATION AND USES OF RENALASE (MONOAMINE OXIDASE C) - The present invention provides for the identification, isolation and uses of mammalian Monoamine Oxidase C (MAO-C), also known as renalase. | 2010-06-03 |
20100136652 | Molecular Conjugate - A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays. | 2010-06-03 |
20100136653 | CREATION OF DIVERSITY IN POLYPEPTIDES - The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. | 2010-06-03 |
20100136654 | METHOD FOR TRANSCRIPTION-DEGRADATION DUAL REGULATION FOR PROTEIN BY ANTIBIOTIC - The present invention provides an expression vector, containing expressibly (a) a polynucleotide encoding a fusion protein of a mutant of a repressor protein, which binds to an antibiotic, and a target protein, and (b) a polynucleotide encoding a protein controlling the transcription of the polynucleotide in (a), the transcription of the polynucleotide in (a) and the degradation of said fusion protein, which is the expression product of the polynucleotide in (a), being controlled inside a cell by the presence or absence of an antibiotic inside the cell. | 2010-06-03 |
20100136655 | FUNGAL ALPHA-AMYLASE VARIANTS - The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants. | 2010-06-03 |
20100136656 | IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID - The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein. | 2010-06-03 |
20100136657 | METHOD FOR STORING SILICA-BASED MATERIAL, PACKAGE PRODUCED WITH THE METHOD AND USE OF PACKAGE FOR PACKAGING OF SILICA-BASED PRODUCTS - A method for storing a water-soluble undissolved silica-based material, optionally with an incorporated functional agent. The method includes immersing the material in an aqueous liquid phase which is initially saturated with a water-soluble silica-based material which is the same or different than the undissolved silica-based material, such that the undissolved silica-based material does not essentially dissolve into the liquid phase during storage, or the proportion of the amount of the initially undissolved silica-based material to the amount of the liquid phase is such that the liquid phase will be saturated or essentially saturated by a dissolved portion of the water-soluble undissolved silica-based material during storage, which dissolved portion of said water soluble undissolved silica-based material is less than 20%. Also disclosed is a storage package which may preferably be used to store functional agents, delivery devices or implants. | 2010-06-03 |
20100136658 | GENERATION OF ONCOLYTIC ADENOVIRUSES AND USES THEREOF - The present invention relates to a method for the production of onolytic adenoviruses having increased potency and their therapeutic applications for cancer. Recombinant adenoviruses and methods to produce them are provided. | 2010-06-03 |
20100136659 | Infectious cDNA Clone of North American Porcine Reproductive and Respiratory Syndrome (PPRS) Virus and Uses Thereof - The invention provides isolated polynucleotide molecules, including plasmids; viral vectors; and transfected host cells that comprise a DNA sequence encoding an infectious RNA sequence encoding a North American PRRS virus; and also North American PRRS viruses encoded thereby. The invention further provides isolated infectious RNA molecules encoding a North American PRRS virus. The invention also provides isolated polynucleotide molecules, infectious RNA molecules, viral vectors, and transfected host cells encoding genetically-modified North American PRRS viruses; and genetically-modified North American PRRS viruses encoded thereby. The invention also provides vaccines comprising such plasmids, RNA molecules, viral vectors, and North American PRRS viruses, and methods of using these vaccines in swine and in other animals. Also provided are isolated polynucleotide molecules, viral vectors, and transfected host cells that comprise a nucleotide sequence encoding a peptide of a North American PRRS virus. These viral vectors and transfected host cell lines are useful in providing peptides to compensate for mutated peptide coding sequences of DNA sequences encoding genetically-modified North American PRRS viruses so that functional virions can be generated. | 2010-06-03 |
20100136660 | METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS - A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods. | 2010-06-03 |
20100136661 | SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS - cells (American Type Culture Collection 700394 | 2010-06-03 |
20100136662 | ENDOTOXIN BINDING BY LACTIC ACID BACTERIA AND BIFIDOBACTERIA - The invention relates to the use of at least one strain of lactic acid bacteria and/or bifidobacteria having hydrophobic surface properties, for the preparation of a composition intended for the prevention or the treatment of endotoxin mediated and/or associated disorders and human or pet food compositions prepared thereof | 2010-06-03 |
20100136663 | PREPARATION OF AN ARTIFICIAL TRANSCRIPTION FACTOR COMPRISING ZINC FINGER PROTEIN AND TRANSCRIPTION FACTOR OF PROKARYOTE, AND A USE THEREOF - The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an | 2010-06-03 |
20100136664 | CARBOXIN RESISTANCE GENE FOR FLAMMULINA VELUTIPES - The present invention pertains to a polynucleotide sequence expressed in | 2010-06-03 |
20100136665 | System and process for reutilization of used disposable diapers - The invention provides a system and a process for reutilization of used paper diapers which enable effective utilization of used paper diapers through processing and can contribute to CO | 2010-06-03 |
20100136666 | GARBAGE DISPOSAL SYSTEM AND MAINTENANCE SCHEDULE CREATING METHOD OF GARBAGE DISPOSAL SYSTEM - A technique of making a maintenance program concerning a garbage disposal system by analyzing at least the weight of garbage brought in by users at least for one week while disposing of the garbage. Before garbage to be disposed of is inputted, the identification information on the user and the date and time of when the garbage is inputted is entered. Only when the identification information agrees with the pre-registered information, the inlet is opened. The weight of the garbage inputted through the inlet is detected with a sensor. The data on the detected weight for each user is stored in a memory. According to the stored data on the weights for one week, an inspection schedule and a bacteria adjustment/supplement schedule of the stirring mechanism and deodorizing mechanism are determined. | 2010-06-03 |
20100136667 | THERMAL TOLERANT AVICELASE FROM ACIDOTHERMUS CELLULOLYTICUS - The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from | 2010-06-03 |
20100136668 | SYSTEMS AND METHODS FOR ISOLATING AND USING CLINICALLY SAFE ADIPOSE DERIVED REGENERATIVE CELLS - Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject. | 2010-06-03 |
20100136669 | MICROCHIP WITH ACCESSIBLE FRONT SIDE - The invention relates to a microchip assembly ( | 2010-06-03 |
20100136670 | Sampling Method and Device - A test device configured for obtaining a sample from within a tube such as the one or more channels of an endoscope. The device can include an elongate sample probe and light blocking material. | 2010-06-03 |
20100136671 | INCUBATION CONTAINER - A laboratory dish comprises an incubation container, containing cultured cells, for observation through a microscope, in which a partitioning member partitions an interior of the incubation container into a plurality of incubation chambers by substantially perpendicular walls. The partitioning member tightly fits to a bottom portion of the incubation container, thereby capable of retaining in separation a liquid inputted in each of the incubation chambers from liquids in the other incubation chambers. A cover member covers an upper portion of each of the incubation chambers partitioned by the partitioning member and tightly fitted to the partitioning member. A whole or part of the cover member is formed of a transparent member, and the upper portion of each incubation chamber is formed with an opening via which to input the liquid. | 2010-06-03 |
20100136672 | Cell Culture Tube and Multiple Roller Tube Cell Culture Apparatus Including the Same - Disclosed is a cell culture tube, having in opposite end walls two respective eccentric openings which communicate with each other through an inner straight passage formed in the cell culture tube, in which the inner straight passage is tilted at an angle relative to a longitudinal axis of the cell culture tube to allow a culture medium to smoothly flow into and out of the inner passage through the openings. Also, provided is a multiple cell culture system using a plurality of the culture tubes. | 2010-06-03 |
20100136673 | CELL/TISSUE CULTURING DEVICE, SYSTEM AND METHOD - A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture. | 2010-06-03 |
20100136674 | Plant for Producing an Oxygen-Containing Additive as an Ecologically Beneficial Component for Liquid Motor Fuels - A plant for producing an oxygen-containing additive for liquid motor fuels comprises an anaerobic fermentation vessel, a gasholder, a system for removal of sulphuretted hydrogen, and a hotwell. The plant further comprises an aerobic fermentation vessel, a device for liquid substance pumping, a device for liquid aeration with an oxygen-containing gas, a removal system of solid mass residue after fermentation, a gas distribution device; a device for heavy gases utilization; a device for ammonia adsorption by water; a liquid-gas mixer; a cavity mixer, a system that serves superficial active and dispersant matters and a cooler; all of these being connected to each other by pipelines. The technical result being the implementation of a process for producing an oxygen containing additive, which after being added to liquid motor fuels, provides an ecologically beneficial component for motor fuels by ensuring the stability of composition fuel properties during long-term storage. | 2010-06-03 |
20100136675 | Incubation and Dry Storage Apparatus - An incubation and dry storage apparatus, especially for samples made of organic material, is provided. | 2010-06-03 |
20100136676 | MODULAR CONTINUOUS PRODUCTION OF MICRO-ORGANISMS - Process for the growing of micro-organism in an open and continuous system further being characterized by means for creating within said system a habitat in which natural, diverse and heterogeneous emicrobial communities can autonomously react and adapt to a changing environment | 2010-06-03 |
20100136677 | Apheresis Tubing Set - An apheresis tubing set comprises a cryocyte bag for collecting cells separated during apheresis. The cryocyte bag may comprise a mixing compartment in fluid communication with a cell storage compartment, wherein the mixing compartment comprises a cryoprotectant port and a cell sample port and wherein the storage and mixing compartments are in fluid communication via a mix conduit. The cryocyte bag may comprise two or more independent cell storage compartments for collecting two or more aliquots of the cells | 2010-06-03 |
20100136678 | METHODS OF DIAGNOSIS OF SPINAL MUSCULAR ATROPHY AND TREATMENTS THEREOF - Methods for treating SMA in a subject comprise administering to the subject a recombinant genetic vector comprising at least one copy of a Stathmin inhibitor. The inhibitor can be a Stathmin expression inhibitor. The inhibitor can be or can encode an antisense or RNAi nucleic acid, such as a siRNA or a shRNA. The copy can be a copy of a host-expressible Stathmin inhibitor or a copy of a host-expressible Stathmin expression inhibitor. Screening methods can comprise the following steps: providing a Stathmin binding agent and a cellular sample from a human subject at least suspected of either having SMA or having a low SMN protein-based condition; contacting the Stathmin binding agent with the cellular sample under conditions in which the agent can specifically bind to Stathmin present in the sample to form complexes, and removing non-specifically bound agent therefrom to leave remaining complexes, and detecting remaining complexes and comparing the level of complexes detected or of the corresponding Stathmin concentration that is present in the sample, to a control level determined from a healthy cellular sample. The detection of an elevated level of complexes or of Stathmin concentration provides a positive screening result. | 2010-06-03 |
20100136679 | Apparatus and Method for Processing Biological Material - The application discloses an apparatus and method for processing biological material, including a suspension of cells. | 2010-06-03 |
20100136680 | BAGLIKE CONTAINER FOR CENTRIFUGATION AND METHOD OF GENE TRANSFER USING THE SAME - Baglike container ( | 2010-06-03 |
20100136681 | CELLULAR COMPOSITIONS FOR THE TREATMENT OF KIDNEY DISEASE AND USES THEREOF - As described in more detail below, the present invention provides cellular compositions for the treatment or prevention of kidney disease. The invention is based, at least in part, on the discovery of a population of cortical peritubular Flk1- and Seal-expressing kidney cells having a high tubulogenic potential. | 2010-06-03 |
20100136682 | Polyvalent RNA-Nanoparticle Compositions - The present invention concerns nanoparticles functionalized with duplex RNA for a variety of uses, including but not limited to gene regulation. More specifically, the disclosure provides a new strategy for conjugating RNA to a nanoparticle to achieve increased stability and activity. | 2010-06-03 |
20100136683 | METHODS FOR ISOLATING CELLS BASED ON PRODUCT SECRETION - The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified. | 2010-06-03 |
20100136684 | Natural IgM Antibodies - The invention provides natural IgM antibodies that may be used to induce inflammatory diseases or disorders such as ischemia-reperfusion injury to create animal models of such inflammatory diseases or disorders. | 2010-06-03 |