23rd week of 2013 patent applcation highlights part 40 |
Patent application number | Title | Published |
20130143191 | SOIL BEHAVIOR SIMULATOR FOR AGRICULTURAL IMPLEMENT BLADES - A soil behavior simulator is provided that includes a particle recirculation system configured to flow particulate material along an agricultural implement blade, and a drive unit configured to drive the agricultural implement blade in rotation. | 2013-06-06 |
20130143192 | METHOD FOR CALCULATING STUDENTS' TIME SPENT ANSWERING QUESTIONS IN AN ON-LINE HOMEWORK SYSTEM - A computer software product and method are provided that calculate students' time spent interacting with an online homework and grading system. A computer software product and method are also provided for real-time capture of students' interactions with an online homework and grading system to produce timely updates to statistics on time spent answering questions. A computer program product and method are further provided to allow statistical data to be presented from WebAssign's online homework system without significantly increasing the IO requirements of the application database server. | 2013-06-06 |
20130143193 | Method of Placing and Absorbent Article - A method for placing an absorbent article is provided. The method includes placing an absorbent article in an undergarment in a manner understood by the individual prior to wearing, capturing a still or video image of the absorbent article on the undergarment, and submitting the still or video image of the absorbent article. The consumer then receives output regarding the placement of the absorbent article. | 2013-06-06 |
20130143194 | Method of Analyzing Video or Image Data of an Absorbent Article - A method for determining the proper placement of an absorbent article on an undergarment is provided. The method includes receiving a still or video image of the absorbent article in the undergarment, determining a central axis, a longitudinal axis, and a central point for the undergarment, the absorbent article, and the stains. The distance between the absorbent article longitudinal axis and the stain longitudinal axis is determined The distance between the absorbent article central axis and the stain central axis is determined. The absorbent article is adjusted on the undergarment according to the distances determined between the stain axis and the absorbent article axis so that the center point of the stain is less than about 20 mm from the center point of the absorbent article. | 2013-06-06 |
20130143195 | LEUKOCYTE PURIFICATION - A method for purifying leukocytes is disclosed. The method can further include recovering the purified leukocytes, or lysing the purified leukocytes. | 2013-06-06 |
20130143196 | METHODS AND MATERIALS FOR PROLONGING USEFUL STORAGE OF RED BLOOD CELL PREPARATIONS AND PLATELET PREPARATIONS - This document provides methods and materials for enhancing the storage capabilities of red blood cell preparations. For example, methods and materials for using CO | 2013-06-06 |
20130143197 | Microfluidic Cell Separation in the Assay of Blood - The present invention is directed to methods for assaying blood samples to quantitate the types of white blood cells present. In addition, the invention includes equipment that can be used for these methods. One feature of the methodology is the use of micro fluidic devices for the separation of white blood cells from red blood cells. | 2013-06-06 |
20130143198 | BLOOD PLASMA LYOPHILIZATION PROCESS - The present invention relates to a lyophilized plasma, resulting from a mixture of plasmas originating from selected donors, which is virally attenuated and compatible with all blood groups and to the process for obtaining same. | 2013-06-06 |
20130143199 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 2013-06-06 |
20130143201 | Crystallographic structure of TcPRACA and uses therefor - A method of identifying a substance that affects the biological activity of a | 2013-06-06 |
20130143202 | COMPOSITIONS AND METHODS FOR THE DETECTION OF HIV-1/HIV-2 INFECTION - This invention relates to compositions and methods or the detection of immunodeficiency virus infection, especially immunodeficiency virus-1 (HIV-1) infection. The invention particularly concerns compositions and methods that may be used in HIV vaccine recipients whose sera may contain vaccine-generated anti-HIV-1 antibodies. | 2013-06-06 |
20130143203 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ADVENTITIOUS VIRUSES - The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis. | 2013-06-06 |
20130143204 | DETERMINATION OF IN VIVO DNA DOUBLE-STRAND BREAK LOCALIZATION AND APPLICATION THEREOF - The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease. | 2013-06-06 |
20130143205 | HIGHLY SENSITIVE METHOD FOR DETECTION OF VIRAL HIV DNA REMAINING AFTER ANTIRETROVIRAL THERAPY OF AIDS PATIENTS - Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”). | 2013-06-06 |
20130143206 | Integrated Analytical System and Method - An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed. | 2013-06-06 |
20130143207 | METHODS AND COMPOSITIONS FOR IDENTIFYING BIOMARKERS USEFUL IN CHARACTERIZING BIOLOGICAL STATES - The present invention relates to methods, compositions, and kits for identifying biomarkers useful in characterizing biological states. In particular, the invention relates to methods and compositions for molecular characterization of biological states by gene expression profiling. The invention also relates to assessing effects of DNA polymorphisms on regulation of transcription. The biomarkers and polymorphisms identified find use in diagnostic and treatment approaches, e.g., some embodiments of the invention provide methods and kits for detecting bronchogenic carcinoma and risks thereof. | 2013-06-06 |
20130143208 | Oligonucleotide Trapping - Novel methods and compositions for identifying one or more factors associated with a nucleic acid sequence (e.g., DNA and/or RNA) of interest are provided. | 2013-06-06 |
20130143209 | PROCESSING OF ANALYTE SUPPORTS WITH OSCILLATING FLUID BY INTERRUPTED ROTATION - Analyte supports such as Western blots, gels, and the like is processed for purposes of detection and analysis, by placing the blot or gel on a plate and causing process fluids to oscillate across the blot or gel by intermittently rotating the plate. The plate can be inclined or flat, divided into sectors to accommodate multiple sheets, and multiple plates can be mounted to a single rotating shaft to process a large number of blots or gels simultaneously under the same protocol. | 2013-06-06 |
20130143210 | METHOD FOR THE PROGNOSIS OF OVARIAN CARCINOMA - The invention relates to a method for determining prognosis of subjects with ovarian carcinoma using a biological sample comprising genomic tumor DNA isolated from the subject. According to the invention, the method comprises the steps of: determining the methylation status of a CpG dinucleotide in a target sequence that is selected from the group consisting of the target sequences as referred to by name in Table 1 in a biological sample isolated from a subject; and deducing from the determined methylation status of the target sequence the prognosis of subject with ovarian carcinoma. The improved prognosis determination of the subject with ovarian carcinoma enables the improved treatment of the said patient. | 2013-06-06 |
20130143211 | PROCESSES AND COMPOSITIONS FOR METHYLATION-BASED ENRICHMENT OF FETAL NUCLEIC ACID FROM A MATERNAL SAMPLE USEFUL FOR NON-INVASIVE PRENATAL DIAGNOSES - Provided are compositions and processes that utilize genomic regions differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuploidies. | 2013-06-06 |
20130143212 | Method of Determining the Abundance of a Target Nucleotide Sequence of a Gene of Interest - The disclosure relates to a method of measuring gene abundance, including obtaining at least two types of amplification product, each of which contains a single additional base sequence and corresponds to each of at least two genes, by amplifying, in one reaction solution, nucleic acids encoding the at least two genes, whose abundances in nucleic acids contained in a subject sample may be different, using a first primer set, which includes at least two types of first primer, each of which is capable of introducing the single additional base sequence to a resulting amplification product and corresponds to each of the at least two genes, and a second primer for amplifying a nucleic acid containing the single additional base sequence; and determining the abundances of the at least two genes based on detected signals corresponding to the abundances of the at least two types of amplification product. | 2013-06-06 |
20130143213 | DETECTION OF GENETIC ABNORMALITIES - The present invention provides assay systems and related methods for determining genetic abnormalities in mixed samples comprising cell free DNA from both normal and putative genetically atypical cells. Exemplary mixed samples for analysis using the assay systems of the invention include samples comprising both maternal and fetal cell free DNA and samples that contain DNA from normal cells and circulating cancerous cells. | 2013-06-06 |
20130143214 | PROSTATE CANCER ASSOCIATED CIRCULATING NUCLEIC ACID BIOMARKERS - The invention provides methods and reagents for diagnosing prostate cancer that are based on the detection of biomarkers in the circulating nucleic acids from a patient to be evaluated. | 2013-06-06 |
20130143215 | Methods of Predicting Methotrexate Efficacy and Toxicity - The present invention provides methods for analyzing genetic and/or metabolite biomarkers to individualize methotrexate (MTX) therapy. For example, the assay methods of the present invention are useful for predicting whether a patient will respond to MTX and/or has a risk of developing toxicity to MTX based upon the genotype of one or more folate pathway genes. The assay methods of the present invention are also useful for optimizing the dose of MTX in a patient already receiving the drug to achieve therapeutic efficacy and/or reduce toxic side-effects based upon the genotype of one or more folate pathway genes. In addition, the assay methods of the present invention are useful for predicting or optimizing the therapeutic response to MTX in a patient based upon the methotrexate polyglutamate and/or folate polyglutamate levels in a sample from the patient. | 2013-06-06 |
20130143216 | Real Time Cleavage Assay - A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured. | 2013-06-06 |
20130143217 | KITS FOR COMPARATIVE TRANSCRIPT ANALYSIS AND MUTANT SEQUENCE ENRICHMENT - A kit to execute a method of simultaneously performing comparative transcript analysis in a multitude of samples. The kit includes a blocking adapter. The blocking adapter includes an inert 3′ end. | 2013-06-06 |
20130143218 | DEVICE FOR AMPLIFYING TARGET NUCLEIC ACID - A device for amplifying a target nucleic acid in a sample containing one or more target nucleic acids may include a substrate assembly comprising a flow channel and an inlet, wherein the inlet is in flow communication with the flow channel and is configured to introduce sample containing one or more target nucleic acids into the flow channel. The device may further include a plurality of moieties disposed at inner surface regions of the flow channel along at least a portion of a length of the flow channel, each of the plurality of moieties being sufficient to respectively hybridize to the one or more target nucleic acids in the sample to facilitate amplification of the one or more target nucleic acids when hybridized. The device is further configured to retain amplified product of the one or more hybridized target nucleic acids at discrete locations proximate the inner surface regions after amplification. | 2013-06-06 |
20130143219 | METHODS AND COMPOSITIONS FOR HIGH YIELD, SPECIFIC AMPLIFICATION - The present invention is directed to methods and compositions for amplifying nucleic acids. Included in the present invention are methods and compositions that amplify nucleic acids with high yield with the formation of unstable target extension products, preferably with minimal or no introduction of allelic bias. Also included in the present invention are high yield, instability primers for use in amplification methods, as multiplexed amplification methods. | 2013-06-06 |
20130143220 | SYSTEMS AND METHODS FOR SAMPLING OF AMPLIFICATION PRODUCTS - The invention provides systems and methods for processing samples. In a method, a reaction card is provided that has a channel network, a valve, and a micropump, all disposed within the card. The reaction card also has a collection well disposed on a surface of the card and a tubular member extending out from the card. A reaction vessel is provided and affixed to the reaction card such that the tubular member is inserted into the reaction vessel. Amplification reaction reagents and a sample are delivered into the reaction vessel, and an amplification reaction is initiated within the reaction vessel, resulting in an amplification product being disposed within the reaction vessel. The valve is opened to atmosphere, and the first micropump is activated to pump an aliquot of reaction product from the reaction vessel into the tubular member, through the channel network, and into the collection well. | 2013-06-06 |
20130143221 | Method and Apparatus for Identifying Defects in a Chemical Sensor Array - In one implementation, a method for operating an apparatus is described. The method includes applying a bias voltage to place a transistor of a reference sensor in a known state. The reference sensor is in an array of sensors that further includes a chemical sensor coupled to a reaction region for receiving at least one reactant. The method further includes acquiring an output signal from the reference sensor in response to the applied bias voltage. The method further includes determining a defect associated with the array if the output signal does not correspond to the known state. | 2013-06-06 |
20130143222 | METHODS FOR TREATING BARRETT'S METAPLASIA AND ESOPHAGEAL ADENOCARCINOMA - Disclosed herein are methods for treating Barrett's metaplasia and esophageal adenocarcinoma and methods for determining mutational load as a predictor of the risk of disease progression from Barrett's metaplasia to esophageal adenocarcinoma. | 2013-06-06 |
20130143223 | METHOD OF DETERMINING KIDNEY TRANSPLANTATION TOLERANCE - The present invention relates to a method of determining an individual's transplantation tolerance by determining the level of a number of biomarkers. The present invention also relates to a kit comprising reagents for detecting the levels of the biomarkers. The present invention also relates to a sensor for detecting the expression levels of a plurality of genes that can be used to determine an individual's transplantation tolerance. | 2013-06-06 |
20130143224 | METHOD FOR DETECTING NUCLEIC ACID, AND DEVICE OR KIT - A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye. | 2013-06-06 |
20130143225 | VARIANT REVERSE TRANSCRIPTASE - The present invention provides a versatile mutant reverse transcriptase with high thermal stability, a nucleic acid thereof and a method for producing a mutant reverse transcriptase, a versatile kits for reverse transcription and detection, a method for improving thermal stability of a nucleic acid-related enzyme, which significantly improves thermal stability of a nucleic acid-related enzyme, and a reverse transcription method, which efficiently performs a reverse transcription. An amino acid residue in a nucleic acid interaction region of a wild-type enzyme is substituted with a positively-charged amino acid residue or a nonpolar amino acid residue, to form a nucleic acid interaction region having a positive effective charge larger than the nucleic acid interaction region of a wild-type enzyme. | 2013-06-06 |
20130143226 | BIOLOGICAL FLUID SAMPLING AND STORAGE APPARATUS FOR REMOTE USE - An apparatus for sampling and storing biological fluids from a human or animal subject is provided. In one embodiment of the present disclosure, the apparatus includes a main body, lancet carrier or hub, lancet, lancet trigger, capillary tube, and sample compartments for collecting and storing dried blood and other bodily fluids. The lancet hub supports a lancet and provides for moving the lancet longitudinally between a first retracted position and a second extended position. The device includes a capillary tube having an internal diameter sized to draw and retain fluid from a contacted source using capillary action. The main body of the apparatus further includes a sample compartment for holding sampling and storage materials. In at least one embodiment of the present disclosure, the sample compartment can be accessed by lifting sample compartment lid. Also included is a new “fan” or “daisy” shaped collection material format for use in collecting and preserving samples. | 2013-06-06 |
20130143227 | REGULATION OF WNT/BETA-CATENIN SIGNALING - A composition and method for detecting the ability of a compound to modulate the activity of LGR4, LGR5, or LGR6 receptors in a cell. LGR4, LGR5, and LGR6 are capable of activating the Wnt/β-catenin signaling system which plays essential roles in embryonic development and in the self-renewal and maintenance of adult stem cells. | 2013-06-06 |
20130143228 | ACTIVITY BASED PROBES (ABPs) INTERACTING WITH GLYCOSIDASES - An activity based probe (ABP) comprising a glycosidase inhibitor, and a detection-group. The ABPs of the inventions are used for diagnosing storage disorder for screening of compounds suitable for preventing and/or treating a storage disorder, for monitoring of therapeutic enzymes for lysosomal storage disorders, and for ultra-sensitive visualization of glycosidase-fusion proteins in molecular imaging. | 2013-06-06 |
20130143229 | REAGENT FOR ASSAYING ANTI-TREPONEMA PALLIDUM ANTIBODY - To provide a reagent for assaying anti- | 2013-06-06 |
20130143230 | MICROFLUIDIC-BASED CELL-CULTURING PLATFORM AND METHOD - A microfluidic-based platform with cultured three-dimensional tissues simulates major human physiological systems for rapid evaluation of individual drugs prior to clinical testing or for personalized medical applications. The platform integrates the circulatory and lymphatic systems in a physiologically correct manner. The physiological systems may be simulated in the platform by microfluidic tissue culture devices which accommodate various tissues and provide integrated microvascular and lymphatic systems. Biomimetic nanofiber meshes or microfiber structures may be used to provide the cells with a physiologically relevant substrate. Each device may have an on-board detection system utilizing optical fiber bundles for microarray multiplexing of biomarkers, label-free SERS measurement of drugs, and microendoscopic confocal imaging of cells and tissues. | 2013-06-06 |
20130143231 | MST1/STK4 PHOSPHO-THREONINE 120 (pMST-T120) ANTIBODY - The present invention relates to prostate cancer (PCa). More specifically, the invention provides a MST1 phosphothreonine (pMST1-T120) antibody that can be used in various assays to study correlations between Mst1 function and T120 site phosphorylation. The present invention can also be used to determine disease development and/or progression of prostate cancer in a subject using the antibodies disclosed herein. | 2013-06-06 |
20130143232 | METHODS FOR THE DETECTION AND MONITORING OF ACUTE MYOCARDIAL INFARCTION - Disclosed herein are methods of detecting and/or prognosing myocardial infarction by detecting a proteolytic fragment of caspase-3 such as the p17 fragment or the p12 fragment. The myocardial infarction can be STEMI or NSTEMI. | 2013-06-06 |
20130143233 | FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY - The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes. | 2013-06-06 |
20130143234 | SAMPLE ANALYZING DEVICE AND SAMPLE ANALYZING METHOD - This invention provides a sample analyzing device and sample analyzing method designed to suppress nonuniform capture of magnetic particles ( | 2013-06-06 |
20130143235 | COMPARATIVE LIGAND MAPPING FROM MHC CLASS I POSITIVE CELLS - Compositions that include isolated, functionally active, recombinantly produced class I HLA trimolecular complexes that include epitopes unique to breast cancer cells are disclosed. | 2013-06-06 |
20130143236 | METHOD FOR DETERMINING SENSITIVITY OF TUMOR CELLS TO DASATINIB AND COMPUTER PROGRAM - The present invention provides a method for determining sensitivity of tumor cells to dasatinib by evaluating inhibitory capacity of a predetermined tyrosine kinase inhibitor in a measurement sample which is prepared from a biological sample containing tumor cells and a computer program. | 2013-06-06 |
20130143237 | COMPOSITIONS AND METHODS FOR PROSTATE CANCER ANALYSIS - The invention provides methods for diagnosing prostate cancer. The invention also provides novel anti-STEAP-1 antibodies and uses thereof. | 2013-06-06 |
20130143238 | PROCEDURE FOR THE GENERATION OF A HIGH PRODUCER CELL LINE FOR THE EXPRESSION OF A RECOMBINANT ANTI-CD34 ANTIBODY - The present invention relates to cell capture assay for the selection of a high producer cell line expressing anti-CD34 antibodies that recognize the CD34 membrane-protein in the cell membrane. The monoclonal antibody secreted by the hybridoma cell line 9C5/9069 binds to human CD34 and is used to isolate stem cells. The DNA sequences encoding for the antibody heavy and light chain have been identified, isolated from the hybridoma cells and cloned into appropriate expression vectors. After co-transfection of the heavy and light chain genes into HEK293T or in CHO cells either conditioned medium or purified antibody were assessed for binding to CD34 protein located in the cell membrane in different cell capture assays. The binding of the antibody to CD34-positive cells could be shown with these assays for several cell lines. | 2013-06-06 |
20130143239 | Melittin Peptide Conjugates And Methods Employing Same - Methods and reagents are disclosed for conducting assays for IgE specific for honey bee venom allergen. A reagent comprises a conjugate of a small molecule linked to a terminal glycine amino acid of a synthetic 26 amino acid melittin peptide. In the method a combination is provided that comprises a sample and the aforementioned reagent. The combination is subjected to conditions for binding of IgE specific for honey bee venom allergen to the reagent to form a complex. One or both of the presence and amount of the complex is detected and related to one or both of the presence and amount in the sample of IgE specific for honey bee venom allergen. | 2013-06-06 |
20130143240 | HOMOARGININE AS A BIOMARKER FOR THE RISK OF MORTALITY - The present invention relates to the field of laboratory diagnostics. Specifically, means and methods for determining the risk of mortality in a patient based on homoarginine and to reduce the risk of mortality by administration of homoarginine are disclosed. Moreover, the present invention relates to the use of homoarginine for the preparation of a medicament for the treatment of a patient having an increased risk of mortality caused by stroke or a cardiac cause. Furthermore, the present application relates to a pharmaceutical composition comprising homoarginine and a composition for foodstuff supplement comprising homoarginine. | 2013-06-06 |
20130143241 | IMMUNOASSAY FOR FREE VITAMIN D - Disclosed is the invention to conduct immuno-adsorption of free 25(OH) vitamin D from blood or blood components, notably serum or plasma, after which the absorbed material is measured. A fluoro-alkyl surfactant is used to enhance the solubility of Vitamin D and allow the measurement of free Vitamin D. The invention thus employs a binding protein to absorb the free 25(OH) vitamin D. Thereafter the binding protein comprising the 25-OH vitamin D is subjected to a competitive binding assay with a labeled vitamin D compound, preferably radiolabeled, fluorescent labeled, luminescent labeled, biotin labeled, gold labeled or enzyme labeled. Alternatively the immunocaptured 25-OH vitamin D can be quantitated by mass spectrometry. | 2013-06-06 |
20130143242 | MONOCLONAL ANTIBODY AGAINST GROUP 2 ALLERGEN OF DERMATOPHAGOIDES PTERONYSSIUNS, HYBRIDOMA CELL LINE PRODUCING THEREOF, STRIP, KIT AND METHOD USING SAID MONOCLONAL ANTIBODY FOR DUST MITE ASSAY - This present invention discloses a monoclonal antibody which specifically recognizes and binds to a epitope of group 2 allergen of | 2013-06-06 |
20130143243 | REAGENT AND REAGENT KIT FOR MEASUREMENT OF FDP, AND MEASUREMENT METHOD - The present invention relates to a reagent for the measurement of FDP comprising a carrier sensitized with at least two monoclonal antibodies selected from three monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent kit comprising the reagent and a method for measurement of FDP using the reagent or reagent kit. | 2013-06-06 |
20130143244 | SOX9 AS A MARKER FOR AGGRESSIVE CANCER - The invention provides methods for determining whether a cancer is or is likely to become aggressive, by detecting the presence of the transcription factor SOX9 in the cytoplasm of cells of the cancer, provided the cancer is not solid pseudopapillary tumor or a melanoma. | 2013-06-06 |
20130143245 | RECOMBINANT PROTEIN CAPABLE OF BINDING SPECIFICALLY AND QUICKLY TO TROPONIN I DERIVED FROM HUMAN MYOCARDIUM - Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65. | 2013-06-06 |
20130143246 | HAND-HELD TEST METER WITH ANALYTICAL TEST STRIP EJECTION MECHANISM - A hand-held test meter for use with an analytical test strip in the determination of an analyte in a bodily fluid sample (e.g., a whole blood sample) includes a housing, with an outer surface, and an analytical test strip ejection mechanism (“ATSEM”). The ATSEM has an actuation button disposed in the outer surface of the housing, a motion amplification and rotation assembly (“MA&RA”) operatively connected to the actuation button and a test strip slider (“TSS”) operatively connected to the MA&RA. The actuation button is configured for movement by a user's digit in a first direction and the MA&RA and TSS are configured to convert the movement in the first direction into amplified movement of the TSS in a second direction with the second direction being rotated with respect to the first direction. Moving the TSS in the second direction from the engaged state to an ejected state ejects the strip. | 2013-06-06 |
20130143247 | VOLATILE ORGANIC COMPOUNDS FOR DETECTING CELL DYSPLASIA AND GENETIC ALTERATIONS ASSOCIATED WITH LUNG CANCER - The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states. | 2013-06-06 |
20130143248 | Force-Clamp Spectrometer And Methods Of Use - The disclosed subject matter relates to a force-clamp spectrometer that enables operation in constant force mode and allows for automated data acquisition and analysis, using feedback electronics and software. The disclosed subject matter also relates to methods of using the force-clamp spectrometer for the measurement of the dynamics of chemical reactions. The methods may include, but are not limited to, the measurement of the dynamics of substrate folding and unfolding, as well as bond cleavage and bond formation. | 2013-06-06 |
20130143249 | METHOD FOR DETERMINING PECTIN CONTENT IN PLANT SAMPLE - Disclosed is a method for determining pectin content in a plant sample, comprising the following steps: 1) adding an acidic alcohol solution to the plant sample, then heating the resulting mixture to reflux in a water bath, followed by a first filtration; 2) soaking the filtered residue obtained from the first filtration with an acidic solution, then heating the resulting mixture to reflux in a water bath, followed by a second filtration, then bringing to volume after cooling, obtaining filtrate for later use; 3) adding an acetic acid/sodium acetate buffer solution to treat the filtered residue obtained from the second filtration, then adding a pectinase solution and heating the resulting mixture under vibration in a water bath, followed by a third filtration to obtain a filtrate for later use; 4) sequentially adding an acetic acid/sodium acetate buffer solution and a pectinase solution to the filtrate obtained in step 2) and heating the resulting mixture under vibration in a water bath to obtain an enzymatic hydrolysate, then adding the filtrate obtained in step 3) to the enzymatic hydrolysate followed by bringing to volume, obtaining a test solution; and 5) drawing the test solution into a continuous flow analyzer to perform analysis. The method disclosed has the advantage of providing accurate analysis results. | 2013-06-06 |
20130143250 | CYCLIC NUCLEOTIDE-SPECIFIC PHOSPHODIESTERASES FROM LEISHMANIA AND USES THEREOF - The present invention relates to novel amino acid and nucleic acid sequences of yclic nucleotide-specific phosphodiesterases from the parasite | 2013-06-06 |
20130143251 | EXPEDITIOUS SYNTHESIS OF UBIQUITINATED PEPTIDE CONJUGATES - The present invention discloses a process for preparing ubiquinated peptide conjugates comprising a ubiquitin peptide residue UR attached at its C-terminus to a substrate peptide via a native isopeptide bond, this process comprising combining Native Chemical Ligation (NCL) and solid phase peptide synthesis (SPPS). Further are disclosed ubiquinated peptide conjugates containing a native isopeptide bond, as well as various uses thereof. | 2013-06-06 |
20130143253 | METHOD FOR QUANTITATIVE MEASUREMENT OF GASTRIC ACIDITY USING 13C CARBONATE SALT - The present invention provides a method for measuring the gastric acidity of a mammal using a | 2013-06-06 |
20130143254 | CO-CULTURE DEVICE ASSEMBLY - An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate. | 2013-06-06 |
20130143255 | ILLUMINATION SIMULATOR FOR ALGAE GROWTH - Systems and methods are provided for using a growth vessel to simulate algae growth and/or productivity in a reference environment, such as an open pond, a closed photobioreactor, or a hybrid system. Based on predicted algae sample trajectories in the reference environment, an illumination profile is developed. An algae sample in the growth vessel can then be exposed to the illumination profile under controlled conditions. Properties of algae in the reference environment can then be characterized based on the sample exposed to the illumination profile. | 2013-06-06 |
20130143256 | LIQUID-BASED METHOD FOR PRODUCING PLANT EMBRYOS - The present invention relates to methods for developing embryos and producing germination-competent embryos using a liquid embryo development media. | 2013-06-06 |
20130143257 | Pressure Monitoring Of Whole Blood Aspirations To Determine Completeness Of Whole Blood Mixing - Methods for use with chemical analyzers aspirate a sample portion from one location to dispense it at a second location, aspirate another sample portion at that second location and dispense it at the first location, and measure the pressure values experienced inside a probe performing the aspirations and dispenses. By comparing the pressure values (or other values indicative of the viscosity or other relevant properties of the sample), the chemical analyzer can determine if the sample is sufficiently mixed or if the sample components remain separated and the method should be repeated. | 2013-06-06 |
20130143258 | DIAGNOSIS OF CANCER - A method is provided comprising, obtaining an infrared (IR) spectrum of a Peripheral Blood Mononuclear Cells (PBMC) sample by analyzing the sample by infrared spectroscopy; and based on the infrared spectrum, generating an output indicative of the presence of a solid tumor or a pre-malignant condition. Other embodiments are also provided. | 2013-06-06 |
20130143259 | Device For Microbiological Analysis - The device for the microbiological analysis of a support adapted to contain microorganisms marked by a fluorophore agent, said agent being adapted to absorb light energy with an absorption spectrum ( | 2013-06-06 |
20130143260 | ALGA IN WHICH PRODUCTION OF PHOTOSYNTHETIC PRODUCTS IS IMPROVED, AND USE FOR SAID ALGA - Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga. | 2013-06-06 |
20130143261 | METHOD FOR THE UTILIZATION OF VINASSE - In order to counteract comprehensively existing vinasse problems, in particular in production of bioethanol from plant raw materials, a method for production of feedstuff from | 2013-06-06 |
20130143262 | METHOD FOR PRODUCING PEPTIDE - Peptides may be produced by allowing (A) a first amino acid or peptide, which is converted into its ionic liquid form, (B) a second amino acid or peptide, and (C) a peptide hydrolase to simultaneously exist in a single reaction system, wherein the first amino acid or peptide, which is converted into its ionic liquid form, is used as both a reaction solvent and a reaction starting material; and forming a peptide bond between the first amino acid or peptide and the second amino acid or peptide. By such a process, it is possible to synthesize a peptide at a high concentration and at a high yield, and the method is excellent for producing peptides on an industrial scale. | 2013-06-06 |
20130143263 | METHOD AND APPARATUS FOR CONVERSION OF CELLULOSIC MATERIAL TO ETHANOL - The present invention provides an apparatus and a method for conversion of cellulosic material, such as chopped straw and corn stover, and household waste, to ethanol and other products. The cellulosic material is subjected to continuous hydrothermal pre-treatment without addition of chemicals, and a liquid and a fibre fraction are produced. The fibre fraction is subjected to enzymatic liquefaction and saccharification. The method of the present invention comprises:
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20130143264 | HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS BY AMULTIPLE TRANSFECTION PROCEDURE OF MAR SEQUENCES - The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method. | 2013-06-06 |
20130143265 | ZCYTOR17 HETERODIMERIC CYTOKINE RECEPTOR POLYNUCLEOTIDES - Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor17-containing multimeric or heterodimer cytokine receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. The present invention also includes methods for producing the multimeric or heterodimeric cytokine receptor, uses therefor and antibodies thereto. | 2013-06-06 |
20130143266 | ANTI-EPHRINB2 ANTIBODIES AND METHODS USING SAME - The invention provides anti-EphrinB2 antibodies, and compositions comprising and methods of using these antibodies. | 2013-06-06 |
20130143267 | VECTORS AND METHODS FOR TRANSDUCING B CELLS - The present disclosure provides compositions and methods for transducing B cells. In particular, the present disclosure provides for vectors and methods for transducing resting B cells and methods for differentiating same to express a transgene of interest. | 2013-06-06 |
20130143268 | METHOD OF PRODUCING THE POLYPEPTIDE FOR TREATING VIRUS-INDUCED CANCER - The present invention relates to methods of producing the polypeptides that are capable of killing cells. The polypeptides comprise a targeting agent covalently attached to a channel-forming moiety. In a preferred embodiment, the channel-forming moiety comprises a colicin and the targeting agent is a reconstructed antibody mimetic derived from monoclonal antibody variants against Epstein-Barr virus gp350/220. | 2013-06-06 |
20130143269 | KIR-Binding Agents and Methods of Use Thereof - The present invention relates to agents and methods that are capable of augmenting NK-mediated killing of target cells by reducing inhibitory KIR signalling without reducing the binding of KIR to HLA-C. As described herein, transduction of negative signaling via KIR, upon binding of KIR to its HLA class I ligand, can involve a ligand-binding induced, conformational reorientation of the KIR molecules allowing interactions to form between adjacent KIRs in specific domains, leading to accelerated clustering. Methods and agents such as monoclonal antibodies for reducing KIR-mediated inhibition of NK cell cytotoxicity without reducing or blocking HLA-binding by, e.g., reducing or blocking dimerization of KIR, are provided. | 2013-06-06 |
20130143270 | PRODUCTION AND PURIFICATION OF IL-29 - The expression vectors and methods using an | 2013-06-06 |
20130143271 | NOVEL EXPRESSION-REGULATING SEQUENCES AND EXPRESSION PRODUCTS IN THE FIELD OF FILAMENTOUS FUNGI - The invention pertains to novel proteins corresponding to | 2013-06-06 |
20130143272 | THERMAL CYCLER WITH VAPOR CHAMBER FOR RAPID TEMPERATURE CHANGES - Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate. | 2013-06-06 |
20130143273 | METHODS FOR NUCLEIC ACID MANIPULATION - A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems. | 2013-06-06 |
20130143274 | METHODS FOR NUCLEIC ACID MANIPULATION - A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems. | 2013-06-06 |
20130143275 | CRUDE BIOLOGICAL DERIVATIVES COMPETENT FOR NUCLEIC ACID DETECTION - The invention relates to methods for the detection of a specific sequence of RNA in a cell or tissue sample. The invention also relates to methods to enzymatically manipulate the RNA in a crude cell lysate in a number of applications. | 2013-06-06 |
20130143276 | Compositions and Methods for Adenylating Oligonucleotides - A method is provided for generating a preparation in which more than 70% of the oligonucleotides are adenylated. The method includes reacting an oligonucleotide with an ATP-sensitive ligase where the ligase is characterized by its ability to efficiently generate adenylated oligonucleotides at ATP concentrations at which ligation and circularization of the oligonucleotide is minimal. | 2013-06-06 |
20130143277 | Methods for Improving the Efficiency of Simultaneous Saccharification and Fermentation Reactions - The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions. | 2013-06-06 |
20130143278 | RECYCLE OF LEACHATE DURING LIGNOCELLULOSIC CONVERSION PROCESSES - The present invention provides a process for producing fermentable sugar or a fermentation product from a lignocellulosic feedstock. The process comprises leaching the lignocellulosic feedstock with an aqueous solution to remove at least potassium salts from the lignocellulosic feedstock and without significantly hydrolyzing hemicellulose and cellulose, thereby producing a leached feedstock and leachate. The leachate is removed from the leachate and concentrated. The leached feedstock is hydrolyzed to produce fermentable sugar, which may be fermented to produce a fermentation broth comprising the fermentation product. The concentrated leachate is recirculated to one or more stages in the process involving alkali addition to adjust the pH of a process stream. | 2013-06-06 |
20130143279 | PROCESS FOR PRODUCING METHIONINE - The present invention relates to a process for producing methionine, comprising a first step of reacting 2-amino-3-buten-1-ol with methanethiol, and a second step of oxidizing 2-amino-4-methylthio-1-butanol obtained in the first step. The present invention also relates to a process for producing 2-amino-4-methylthio-1-butanol, comprising a step of reacting 2-amino-3-buten-1-ol with methanethiol. | 2013-06-06 |
20130143280 | FERMENTATIVE VITAMIN C PRODUCTION - The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C. | 2013-06-06 |
20130143281 | Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from | 2013-06-06 |
20130143282 | ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL PRODUCTION - Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties. | 2013-06-06 |
20130143283 | Integrated Bio-Digestion Facility - Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester. | 2013-06-06 |
20130143284 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. | 2013-06-06 |
20130143285 | METHOD FOR DILUTE ACID PRETREATMENT OF LIGNOCELLULOSIC FEEDSTOCKS - The present invention relates to a process for the conversion of a lignocellulosic feedstock involving acid pretreatment. The process comprises the steps of treating the lignocellulosic feedstock with alkali at a pH of between about 8.0 and about 12.0 so as to dissolve acetyl groups present on said lignocellulosic feedstock, while converting less than about 10% of the xylan present in the lignocellulosic feedstock to xylose and less than about 10% of the cellulose to glucose, thereby producing an alkali conditioned feedstock. The alkali conditioned feedstock is then pretreated at a temperature of about 160° C. to about 250° C., at a pH of about 0.5 to about 2.5 for about 0.5 to about 10 minutes so as to hydrolyze about 80 to 100% of the xylan and about 3 to about 15% of the cellulose to produce an acid pretreated feedstock comprising cellulose. The cellulose in the pretreated feedstock can be hydrolyzed to glucose with cellulase and the glucose can be fermented to produce a fermentation product. | 2013-06-06 |
20130143286 | BACILLUS COAGULANS STRAINS AND THEIR APPLICATIONS IN L-LACTIC ACID PRODUCTION - The invention is concerned with the strains of | 2013-06-06 |
20130143287 | PROCESS FOR THE CONVERSION OF BIOMASS OF PLANT ORIGEN, AND A COMBUSTION PROCESS - The invention provides a process for the conversion of biomass into a biomass product which is suitable for use as a fuel. The biomass is of plant origin and comprises microorganisms naturally occurring in the biomass. The process comprises—preparing a slurry by dispersing the biomass comprising the naturally occurring microorganisms in an aqueous liquid, maintaining the slurry at conditions suitable for aerobic digestion by the microorganisms to obtain a slurry comprising the biomass product as a dispersed solid phase, and—recovering the biomass product. The recovering comprises washing and drying the biomass product. The invention also provides a combustion process. | 2013-06-06 |
20130143288 | ISOLATED BACTERIA, METHODS FOR USE, AND METHODS FOR ISOLATION - Isolated bacteria are disclosed. The isolated bacteria are aerotolerant and can produced butanol. Methods are disclosed to produce generated chemicals. Methods are disclosed to isolate aerotolerant bacteria. | 2013-06-06 |
20130143289 | Pre-Treatment of Cellulosic Material - A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO | 2013-06-06 |
20130143290 | SYSTEM FOR THE TREATMENT OF BIOMASS TO FACILITATE THE PRODUCTION OF ETHANOL - A method for treating biomass to be supplied to a fermentation system for the production of a fermentation product is disclosed. The method comprises the steps of pre-treating the biomass into pre-treated biomass; separating the pre-treated biomass into a first component comprising glucan and a second component comprising sugars; providing a combined component comprising at least a portion of the first component and at least a portion of the second component; and treating the combined component of the pre-treated biomass into a treated component comprising glucose by application of an enzyme formulation. A system for treating biomass to be supplied to a fermentation system for the production of a fermentation product is also disclosed. The system comprises an apparatus configured to pre-treat the biomass; a separator configured to separate the pre-treated biomass; and a vessel configured to contain a combined component. | 2013-06-06 |
20130143291 | PRODUCTION OF MONOTERPENES - The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase. | 2013-06-06 |
20130143292 | DEVICE AND METHOD FOR THE ANAEROBIC DIGESTION OF ORGANIC MATERIAL TO BIOGAS BY MEANS OF MICRO-ORGANISMS - The invention relates to a device and method for the anaerobic digestion of organic material to biogas by means of microorganisms. The device and method intensify the anaerobic digestion process by using three separate reactors wherein a hydrolysis process, a first digestion process of a separated solid fraction, and a second digestion process of a separated liquid fraction take place respectively. Membranes and/or mechanical separation means are used to separate the fractions. This keeps the microorganisms, such as microbes, in the reactors. The obtained intensification of the anaerobic digestion process enables to operate with smaller tanks, which leads to lower building costs. The process also makes it possible to precipitate heavy metals for extraction. | 2013-06-06 |