24th week of 2017 patent applcation highlights part 24 |
Patent application number | Title | Published |
20170166860 | METHODS AND APPARATUS FOR PARTICLE AGGREGATION USING ACOUSTIC STANDING WAVES - Methods for generating particulate clusters and nodal trapping lines having desired widths are disclosed. The devices include an acoustic chamber having an inlet and an outlet. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave that generates particulate clusters separated by a channel of fluid running therebetween and creates nodal trapping lines. The frequency of the multi-dimensional acoustic standing wave can be selectively tuned so as to selectively control at least one of (a) a width of each particulate cluster, or (b) a width of each channel of fluid. The frequency of the multi-dimensional acoustic standing wave can also be selectively tuned so as to selectively control the width of each nodal trapping line. Also disclosed are particulate clusters separated by a channel of fluid, wherein a ratio of the widths of the particulate clusters and the channel of fluid can be varied as desired. | 2017-06-15 |
20170166861 | TECHNOLOGY FOR SUSTAINING PLURIPOTENCY AND IMPROVED GROWTH OF STEM CELLS IN CULTURE - A method of culturing pluripotent stem cells is provided. The method includes culturing pluripotent stem cells in a pluripotent stem cell culture medium supplemented with an additive, where the additive includes a source of acetate ions, a carboxylic acid, or a physiologically acceptable salt of the carboxylic acid, or a combination of these substances, in an amount effective to maintain the pluripotent stem cells in culture in an undifferentiated pluripotent state. Also included are pluripotent stem cell culture media and methods of making such media. | 2017-06-15 |
20170166862 | SUBSTRATE FOR A THREE-DIMENSIONAL CELL CULTURE, ITS PREPARATION AND USE - A layered material suitable as substrate for a three-dimensional cell culture includes a transparent carrier layer and a transparent conductive layer as well as a photoconductive layer comprising titanium oxide phthalocyanine. A method for producing the layered material and an article comprising it and a receiving unit are disclosed. A mold-free method is provided for forming a three-dimensional hydrogel as well as for forming a three-dimensional cell culture by using the layered material or article. Uses of the layered material and of the three-dimensional hydrogel and three-dimensional cell culture are also disclosed. The layered material, cell culture and methods provided overcome significant drawbacks of currently known technologies and allows for forming various three-dimensional patterns with high flexibility and controllability via fast and relative simple and inexpensive methods. The provided layered material and article comprising it can be produced and assembled with advantageously small dimensions. | 2017-06-15 |
20170166863 | POSTPARTUM CELLS DERIVED FROM PLACENTAL TISSUE, AND METHODS OF MAKING AND USING THE SAME - Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications. | 2017-06-15 |
20170166864 | POSTPARTUM CELLS DERIVED FROM PLACENTAL TISSUE, AND METHODS OF MAKING AND USING THE SAME - Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications. | 2017-06-15 |
20170166865 | ELECTRIC OVUM DENUDING DEVICE AND OVUM DENUDING METHOD - An electric ooycte denuding device and an ooycte denuding method are provided. The electric oocyte denuding device includes an oocyte denuding pipette: a manipulating handle; and a drive module, a control module, a display module, a power module, a memory, a bulb, and/or a voice module, and/or a pressure sensor, and/or a control box, and/or a foot-operated switch controller, which form an integrated type or a seperated type electric ooycte denuding device. A stepper motor provides the power for blowing and sucking to denude the granular cells surrounding an oocyte. | 2017-06-15 |
20170166866 | METHODS OF IMPROVING VECTOR TRANSDUCTION EFFICIENCY INTO T LYMPHOCYTES - Provided herein are methods for enhancing the transduction efficiency of vectors into cells, e.g., primary human T lymphocytes. | 2017-06-15 |
20170166867 | 2,4,5-TRI-SUBSTITUTED AZOLE-BASED CASEIN KINASE 1 INHIBITORS AS INDUCERS FOR CARDIOMYOGENESIS - This invention relates to a method for inducing or enhancing the differentiation of pluripotent stem cells into cardiomyocyte via casein kinase 1 inhibition said method comprising culturing the stem cells in the presence of a medium comprising a casein kinase 1 inhibitor of the formula (I) or (II) or a stereoisomer, tautomer, or a salt thereof wherein R | 2017-06-15 |
20170166868 | Media for Culturing, Preserving, and Administering Regenerative Cells - A culture media, media supplement, or soluble matrix for cryopreservation or enhanced regenerative cell growth in culture and maintenance of multi-lineage differentiation potentiation. The inventive culture media, media supplement, or soluble matrix comprises a GAG composition comprising a sulfated GAG, such as chondroitin sulfate. A soluble matrix, a cell administration package or kit comprising the soluble matrix and a device for cell administration, and a method of use thereof, for administration of regenerative cells for treating a joint disease or other weakened or damaged tissue comprising the specified GAG compositions are further provided. | 2017-06-15 |
20170166869 | BIOLOGICALLY OPTIMIZED ADULT MESENCHYMAL STEM CELLS - The invention disclosed provides populations and compositions of mesenchymal stem and progenitor cells derived from adult tissues that are subjected to a process or processes designed to enhance a biological mechanism prior to implantation of the cells for therapeutic use. The processes involve preconditioning of said mesenchymal stem cells with a physiological stressor in vitro, so as to enhance therapeutically significant biological properties. Specific embodiments of the invention include stimulation of angiogenic and immune modulatory properties by preconditioning with interferon-gamma, as well as other physiologically-relevant stressors such as activation of toll like receptors. Immunologically-relevant properties of preconditioned cells including the treatment of cytokine storm associated with sepsis, or viral infection. In the angiogenic embodiment of the invention, cells are used for the treatment of peripheral artery disease, its advanced form, critical limb ischemia, or ischemic heart failure. | 2017-06-15 |
20170166870 | HUMAN HEPATIC 3D CO-CULTURE MODEL AND USES THEREOF - The invention in general relates to human hepatic 3D co-culture models, more in particular 3D spheroid co-cultures of human hepatocyte-like cells and hepatic stellate cells. Furthermore, the invention provides a method for obtaining such co-cultures, as well as the use of said co-cultures in the identification of pro-fibrotic and/or anti-fibrotic compounds. | 2017-06-15 |
20170166871 | PRODUCTION METHOD FOR NON-ENVELOPED VIRUS PARTICLES - Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient. | 2017-06-15 |
20170166872 | PROCESS FOR THE PURIFICATION OF POLIOVIRUS FROM CELL CULTURES - The disclosure provides methods for poliovirus purification from crude cell culture harvests using a detergent followed by a clarification step. | 2017-06-15 |
20170166873 | PURIFICATION OF PROTEINS WITH CATIONIC SURFACTANT - The subject invention provides a method for purifying a target protein from a mixture comprising the target protein and contaminating protein, comprising the steps of exposing the mixture to an effective amount of a cationic surfactant such that the contaminating protein is preferentially precipitated and recovering the target protein. Proteins purified according to the method of the invention are also provided. | 2017-06-15 |
20170166874 | Hyperactive Piggybac Transposases - The present invention provides PiggyBac transposase proteins, nucleic acids encoding the same, compositions comprising the same, kits comprising the same, non-human transgenic animals comprising the same, and methods of using the same. | 2017-06-15 |
20170166875 | METHODS RELATING TO NUCLEIC ACID SEQUENCE EDITING - The technology described herein is directed to engineered endonucleases whose activity is restricted to certain phases of the cell cycle. Provided herein are compositions and methods relating to such engineered endonucleases. | 2017-06-15 |
20170166876 | Alpha-amylase variant with altered properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase. | 2017-06-15 |
20170166877 | DUAL CONTROLS FOR THERAPEUTIC CELL ACTIVATION OR ELIMINATION - The technology relates in part to methods for controlling the activity or elimination of therapeutic cells using molecular switches that employ distinct heterodimerizer ligands, in conjunction with other multimeric ligands. The technology may be used, for example to activate or eliminate cells used to promote engraftment, to treat diseases or condition, or to control or modulate the activity of therapeutic cells that express chimeric antigen receptors or recombinant T cell receptors. | 2017-06-15 |
20170166878 | COMPOSITIONS OF ADENOSINE DEAMINASE-2 (ADA2), VARIANTS THEREOF AND METHODS OF USING SAME - Provided are variant adenosine deaminase 2 (ADA2) proteins, conjugates thereof and compositions containing the proteins and/or conjugates. Also provided are methods and uses of the ADA2 proteins or conjugates for treating diseases and conditions, such as a tumor or cancer, and in particular any disease or condition associated with elevated adenosine or other associated marker. | 2017-06-15 |
20170166879 | NOVEL (R)-HYDROXYNITRILE LYASE - The objective of the present invention is to provide a (R)-hydroxynitrile lyase which is more stable than a hydroxynitrile lyase derived from a plant, a gene which encodes the hydroxynitrile lyase and by which heterologous expression is possible, and a method for producing the hydroxynitrile lyase. The (R)-hydroxynitrile lyase according to the present invention is characterized in having the specific amino acid sequence such as the amino acid sequence of SEQ ID NO: 3. | 2017-06-15 |
20170166880 | NOVEL CARBON FIXATION CYCLE AND USE THEREOF - The present invention relates to a novel carbon dioxide fixation cycle synthesizing a carbohydrate product from carbon dioxide in vitro. In addition, the present invention relates to a unit or a composition carrying out carbon dioxide fixation in cyclic manner. Additionally, the present invention relates to a method to fix carbon dioxide or a method to produce glyoxylate from the carbon dioxide fixation cycle. The present carbon dioxide fixation cycle is not found in natural world, and we found that, when the novel carbon dioxide fixation cycle is used, only three ATP molecules are consumed to fix one carbon dioxide molecule, and thus novel carbon dioxide fixation cycle has an energy conversion efficiency approximately 2.5 times higher than that of the Calvin cycle. | 2017-06-15 |
20170166881 | POLY-L-LACTIC ACID (PLLA) MICROTUBE ARRAY MEMBRANE-IMMOBILIZED YEAST CELLS FOR BIOETHANOL FERMENTATION - The invention uses a highly porous MTAM to immobilize yeast cells for bioethanol fermentation, a first in bioethanol production. The invention also optimizes the conditions to prepare MTAM-immobilized cells and evaluates their potential for batch bioethanol fermentation. | 2017-06-15 |
20170166882 | METHOD FOR MOBILIZING IMMOBILIZED CELLS - The invention concerns the fields of microtechnologies, biology and medicine and relates to a method for producing mobility in immobile cells as can be used, for example, for in vivo or in vitro fertilization. | 2017-06-15 |
20170166883 | ENHANCED MICROBIAL ELECTROSYNTHESIS BY USING CO-CULTURES - A method of microbial electrosynthesis using co-cultures is disclosed. In particular, the invention relates to a method of microbial electrosynthesis utilizing a microbial strain capable of electron uptake from an electrode to produce hydrogen or formate in co-culture with a microbial production strain, such as a methanogen, acetogen, or other microorganism capable of synthesizing valuable products from carbon dioxide and hydrogen or formate. | 2017-06-15 |
20170166884 | Coated Cell Culture Apparatus and Methods of Use - Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner. | 2017-06-15 |
20170166885 | NUCLEIC ACID EXTRACTION USING ORGANIC SOLVENTS TO REMOVE INHIBITORS - Nucleic acid amplification tests have been widely used in clinical laboratories. Nucleic acid extraction from biological materials is challenging because different unfavorable substances may co-extract and inhibit downstream applications. The present invention relates to a composition of and a method for treating the sample prior, during or post extraction of nucleic acid. More specifically, the claimed invention relates to a composition of and a method for using low concentrations of common organic solvents to remove inhibitors of nucleic acid amplification. The present invention can be used for extracting nucleic acids (DNA/RNA) from bacteria, viruses, parasites, and other biological materials or matrices, including but not limit to, stool samples, body fluids, plants and cultures. The method is rapid, low-cost, and easy to use in a laboratory setting. The nucleic acid extracted in accordance with the invention can be used for nucleic acid amplification reactions. | 2017-06-15 |
20170166886 | PEPTIDE LIBRARIES - The invention relates to novel libraries of linear and cyclic peptides, and methods of generating and screening such libraries for biological, pharmaceutical and other uses. | 2017-06-15 |
20170166887 | OB-FOLD USED AS SCAFFOLD FOR ENGINEERING NEW SPECIFIC BINDERS - The present invention pertains to the field of protein engineering, and provides means for obtaining stable molecules that specifically bind to a target selected amongst a large variety of ligands families. In particular, the present invention provides methods for obtaining a molecule specifically binding to a target of interest, through a combinatorial mutation/selection approach with an OB-fold protein as a starting molecule. In particular, the target of interest can be of a different chemical nature form that of the native target of the OB-fold protein used as the starting molecule. | 2017-06-15 |
20170166888 | SECRETED PROTEINS - The invention provides human secreted proteins (SECP) and polynucleotides which identify and encode SECP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of SECP. | 2017-06-15 |
20170166889 | System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells - Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences. | 2017-06-15 |
20170166890 | Dnazyme-nanoparticle conjugates and methods of use thereof - The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components. | 2017-06-15 |
20170166891 | METHODS OF TREATING A METABOLIC SYNDROME BY MODULATING HEAT SHOCK PROTEIN (HSP) 90-BETA - The invention provides methods of treatment of metabolic syndrome with HSP90 inhibitors, particularly HSP90β inhibitors. The invention provides methods of diagnosis and monitoring of metabolic syndrome using HSP90, particularly HSP90β, expression and activity level. | 2017-06-15 |
20170166892 | Methods and Pharmaceutical Compositions for the Treatment of Cardiovascular Fibrosis - The present invention relates to methods and pharmaceutical compositions for the treatment of cardiovascular fibrosis. In particular, the present invention relates to an inhibitor of Neutrophil Gelatinase-Associated Lipocalin (NGAL) activity or expression for use in a method for treating or preventing cardiovascular fibrosis in a subject in need thereof. | 2017-06-15 |
20170166893 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2017-06-15 |
20170166894 | APTAMERS FOR TOPICAL DELIVERY - Aptamers for topical delivery and methods for topical use of aptamers are described. Aptamers that bind to interleukin (IL)-23 (IL-23 aptamers) and methods of using such aptamers are also described. | 2017-06-15 |
20170166895 | NUCLEIC ACID COMPOUNDS FOR BINDING TO COMPLEMENT COMPONENT 3 PROTEIN - Described herein are aptamers capable of binding to human complement component 3 (C3) protein; compositions comprising a C3 binding aptamer with a C3-Protein; and methods of making and using the same. | 2017-06-15 |
20170166896 | Treatment of Liver Cancer through Embolization Depot Delivery of BORIS Gene Silencing Agents - Methods of treatment of cancer are disclosed through administration of siRNA and shRNA sequences silencing BORIS gene and isoforms thereof. One embodiment of the invention discloses pharmaceutical compositions and kits for modifying the palliative procedure of transarterial chemoembolization so as to promote uptake of gene silencing inducing agents into the hepatic cancer microenvironment. By selectively administering under localized increased pressure, enhanced uptake of gene silencing agents is achieved, thus increasing targeting of tumor cells, particularly stem cells. | 2017-06-15 |
20170166897 | COMPOSITIONS AND METHODS FOR TARGETING O-LINKED N-ACETYLGLUCOSAMINE TRANSFERASE AND PROMOTING WOUND HEALING - The presently disclosed subject matter provides compounds, compositions, and methods for targeting UDP-N-acetylglucosamine polypeptide β-N-acetylglucosaminyl transferase (OGT). Further, the presently disclosed subject matter provides compounds, compositions, and methods for promoting wound healing. | 2017-06-15 |
20170166898 | SIRNA STRUCTURES FOR HIGH ACTIVITY AND REDUCED OFF TARGET - This invention provides compounds, compositions and methods for modulating the expression of target genes using RNA interference. RNAi structures and molecules of this invention can be used for modulating or silencing the expression of genes, with high levels of RNAi activity and reduced off target actions. Advantageous structures include siRNAs targeted to any gene having one or more 2′-deoxy nucleotides located in the seed region. The RNA interference molecules can be used in methods for preventing or treating diseases. | 2017-06-15 |
20170166899 | COMPOSITIONS AND METHODS FOR MODULATING GROWTH HORMONE RECEPTOR EXPRESSION - The present embodiments provide methods, compounds, and compositions for treating, preventing, ameliorating a disease associated with excess growth hormone using antisense compounds oligonucleotides targeted to growth hormone receptor (GHR). | 2017-06-15 |
20170166900 | PEPTIDE NUCLEIC ACID MONOMERS AND OLIGOMERS - The invention relates to new peptide nucleic acid monomers and peptide nucleic acid oligomers comprising a dialkylamine side chain substituted with phosphonic acid ester group(s) or phosphonic acid group(s), and to the uses thereof. | 2017-06-15 |
20170166901 | METHOD FOR OPENING TIGHT JUNCTIONS - The present invention is directed to a method and use of RNA interference (RNAi) for the transient, reversible and controlled opening of the tight junctions of the blood brain barrier and/or the blood retinal barrier. This method may be used in the treatment of many diseases and disorders which require the opening of the blood brain barrier and/or blood retinal barrier. Such methods generally involve the use of an RNAi-inducing agent, such as siRNA, miRNA, shRNA or an RNAi-inducing vector whose presence within a cell results in production of an siRNA or shRNA, targeting tight junction proteins to open the blood brain barrier and/or blood retinal barrier. | 2017-06-15 |
20170166902 | RECOMBINANT MICROORGANISMS HAVING A METHANOL ELONGATION CYCLE (MEC) - Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms. | 2017-06-15 |
20170166903 | INDUCIBLE DNA BINDING PROTEINS AND GENOME PERTURBATION TOOLS AND APPLICATIONS THEREOF - The present invention generally relates to methods and compositions used for the spatial and temporal control of gene expression that may use inducible transcriptional effectors. The invention particularly relates to inducible methods of altering or perturbing expression of a genomic locus of interest in a cell wherein the genomic locus may be contacted with a non-naturally occurring or engineered composition comprising a deoxyribonucleic acid (DNA) binding polypeptide. | 2017-06-15 |
20170166904 | METHOD FOR PRODUCING VECTOR IN WHICH FOREIGN GENE HAS BEEN INTRODUCED - The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D. | 2017-06-15 |
20170166905 | STABILIZATION OF POLY(A) SEQUENCE ENCODING DNA SEQUENCES - The present invention relates to nucleic acid molecules containing poly (dA:dT) regions which are stabilized in | 2017-06-15 |
20170166906 | METHODS OF MAKING MINICIRCLES - The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby to form a (i) minicircle comprising the nucleic acid sequence and (ii) a miniplasmid comprising the remainder of the parent plasmid. One recombination site is modified at the 5′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, both modified sites being located in the minicircle after recombination. This favours the formation of minicircle. | 2017-06-15 |
20170166907 | NON-REPLICATIVE TRANSDUCTION PARTICLES AND TRANSDUCTION PARTICLE-BASED REPORTER SYSTEMS - Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules. | 2017-06-15 |
20170166908 | VECTOR REPLICABLE IN E. COLI AND CELL OF GENUS KOMAGATAEIBACTER, CELL INCLUDING THE SAME, AND METHOD OF USING THE SAME - Provided are a vector replicable in | 2017-06-15 |
20170166909 | IMPROVED STRAINS OF AGROBACTERIUM TUMEFACIENS FOR TRANSFERRING DNA INTO PLANTS - The present invention relates to | 2017-06-15 |
20170166910 | PICHIA PASTORIS STRAINS FOR PRODUCING PREDOMINANTLY HOMOGENEOUS GLYCAN STRUCTURE - Disclosed herein are novel | 2017-06-15 |
20170166911 | TECHNIQUES FOR TRANSFECTING PROTOPLASTS - The invention relates to a method for the introduction of one or more molecules of interest in a plant cell protoplast by providing plant cell protoplasts, performing a first transfection of the plant cell protoplast with a composition that is capable of altering the regulation of one or more pathways selected from the group consisting of Mismatch Repair System and Non-Homologous End Joining and/or a composition that is capable of introducing DSBs, performing a second transfection of the plant cell protoplast with one or more molecules of interest such as mutagenic oligonucleotides and allowing the cell wall to form. | 2017-06-15 |
20170166912 | COMPOSITIONS AND METHODS FOR SITE DIRECTED GENOMIC MODIFICATION - The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage. | 2017-06-15 |
20170166913 | REDUCING LEVELS OF NICOTINIC ALKALOIDS IN PLANTS - Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants. | 2017-06-15 |
20170166914 | Reduction of Tobacco-Specific Nitrosamines Using Genetic Modification to Elevate Production of Native Antioxidants in Tobacco - Cured tobacco having a lower concentration of tobacco specific nitrosamines, can be produced from a transgenic tobacco plant having a heterologous nucleotide sequence encoding a polypeptide that catalyzes production of active oxygen species. The heterologous nucleotide sequence is operably coupled to a regulatory nucleotide sequence that directs expression of the polypeptide. The regulatory nucleotide sequence can include constitutive and/or inducible promoter elements. | 2017-06-15 |
20170166915 | PLANT REGULATORY ELEMENTS AND USES THEREOF - The present invention provides novel regulatory elements for use in plants. The present invention also provides DNA constructs containing these novel regulatory elements; transgenic cells, plants, and seeds containing these novel regulatory elements; and methods for preparing and using the same. | 2017-06-15 |
20170166916 | Genetic Markers Associated with Drought Tolerance in Maize - The present invention relates to methods and compositions for identifying, selecting and/or producing a maize plant or maize plant part having increased yield under non-drought conditions, increased yield stability under drought conditions, and/or increased drought tolerance. A maize plant or maize plant part, including any progeny and/or seeds derived from a maize plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided. | 2017-06-15 |
20170166917 | NUCLEOTIDE SEQUENCES AND POLYPEPTIDES ENCODED THEREBY USEFUL FOR MODIFYING PLANT CHARACTERISTICS IN RESPONSE TO COLD - Methods and materials for modulating cold tolerance levels in plants are disclosed. For example, nucleic acids encoding cold tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased levels of cold tolerance and plant products produced from plants having increased cold tolerance levels. | 2017-06-15 |
20170166918 | HPPD VARIANTS AND METHODS OF USE - In the present invention, HPPD enzymes and plants containing them showing a full tolerance against several classes of HPPD-inhibitors are described. A set of HPPD enzymes have been designed which have either no or only a significantly reduced affinity to HPPD inhibitors and, at the same time, the rate of dissociation of the HPPD inhibitors of the enzyme is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. | 2017-06-15 |
20170166919 | ACETYL CO-ENZYME A CARBOXYLASE HERBICIDE RESISTANT PLANTS - The present invention provides for compositions and methods for producing crop plants that are resistant to herbicides. In particular, the present invention provides for wheat plants, plant tissues and plant seeds that contain altered acetyl-CoA carboxylase (ACCase) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ACCase protein. | 2017-06-15 |
20170166920 | PLANTS WITH ENHANCED RESISTANCE TO PHYTOPHTHORA - Methods and compositions for enhancing the resistance of plants to oomycete plant pathogens are provided. The methods involve decreasing in the plant or part thereof the level of a remorin, particularly a remorin that is known to occur in the extrahaustorial membrane that is formed in a host plant in response to an infection by one or more oomycete plant pathogens. Compositions comprise plants and plant cells with a reduced level and/or activity of at least one remorin in the plant or part thereof when compared to a control plant or part thereof. Additionally provided are methods for using the plants in agriculture to limit diseases caused by oomycete pathogens | 2017-06-15 |
20170166921 | NOVEL INSECTICIDAL PROTEINS FROM PLANTS - Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. | 2017-06-15 |
20170166922 | COTTON TRANSGENIC EVENT MON 88702 AND METHODS FOR DETECTION AND USES THEREOF - The invention provides a transgenic | 2017-06-15 |
20170166923 | PROTEIN EXPRESSION FROM MULTIPLE NUCLEIC ACIDS - The current invention reports a method for the recombinant production of a secreted heterologous immunoglobulin in a CHO cell comprising the following steps: i) providing a CHO cell, which is adapted to growth in suspension culture, adapted to growth in serum-free medium, mycoplasma free, and virus free, ii) providing a vector comprising a prokaryotic origin of replication, a first nucleic add conferring resistance to a prokaryotic selection agent, a second nucleic acid encoding the heavy chain of said heterologous immunoglobulin, a third nucleic acid encoding the light chain of said heterologous immunoglobulin, a fourth nucleic acid conferring resistance to a eukaryotic selection agent, iii) transfecting said CHO cell, wherein said transfecting comprises a) transfecting said CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a first eukaryotic selection agent, h) selecting a CHO cell by growth in cultivation medium containing said first eukaryotic selection agent, c) transfecting said selected CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a second eukaryotic selection agent different to said first eukaryotic selection agent, d) selecting a CHO cell by selected growth in cultivation medium containing said first and said second eukaryotic selection agent, iv) cultivating said transfected CHO cell in a medium in the presence of said first and second eukaryotic selection agent, under conditions suitable for the expression of said second, and third nucleic acid, and v) recovering said secreted heterologous immunoglobulin from the cultivation medium. | 2017-06-15 |
20170166924 | METHODS FOR PRODUCING AND USING IN VIVO PSEUDOTYPED RETROVIRUSES - The present invention provides novel pseudotyped retroviral vectors that can transduce human and other cells. Vectors are provided that are packaged efficiently in packaging cells and cell lines to generate high titer recombinant virus stocks expressing novel envelope glycoproteins. The present invention further relates to compositions for gene therapy. | 2017-06-15 |
20170166925 | RECOMBINANT AAV VECTORS USEFUL FOR REDUCING IMMUNITY AGAINST TRANSGENE PRODUCTS - The disclosure in some aspects, relates to nucleic acids, compositions and kits useful for gene therapy with reduced immune response to transgene products. | 2017-06-15 |
20170166926 | TARGETING PEPTIDES FOR DIRECTING ADENO-ASSOCIATED VIRUSES (AAVs) - Disclosed herein are peptide sequences capable of directing adeno-associated viruses (AAV) to target specific environments, for example the nervous system and the heart, in a subject. Also disclosed are AAVs having non-naturally occurring capsid proteins comprising the disclosed peptide sequences, and methods of using the AAVs to treat diseases. | 2017-06-15 |
20170166927 | ISOLATION OF NOVEL AAV'S AND USES THEREOF - The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA. | 2017-06-15 |
20170166928 | Compositions And Methods For Genetically Modifying Yeast - The present invention provides compositions and methods for genetically modifying yeast cells using a | 2017-06-15 |
20170166929 | METHOD OF CONVERTING MARINE FISH WASTE TO BIOMETHANE - Methods employed for the discovery, enrichment, and characterization of a marine consortium of fermentative and methanogenic microorganisms developed from the solid waste digestor of a fully contained, land-based, marine recirculating aquaculture system are described. The methanogenic microbial consortium discovered is capable of reducing over 90% of marine fish waste in an aquaculture system to biomethane and carbon dioxide at saline concentrations found in marine aquaculture. Systems and methods for the treatment of marine fish waste utilizing the methanogenic marine consortium are also disclosed. | 2017-06-15 |
20170166930 | SYNGAS BIOMETHANATION PROCESS AND ANAEROBIC DIGESTION SYSTEM - An anaerobic digester is fed a feedstock, for example sludge from a municipal wastewater treatment plant, and produces a digestate. The digestate is dewatered into a cake. The cake may be dried further, for example in a thermal drier. The cake is treated in a pyrolysis system to produce a synthesis gas and biochar. The gas is sent to the same or another digester to increase its methane production. The char may be used as a soil enhancer. | 2017-06-15 |
20170166931 | MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO - The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. | 2017-06-15 |
20170166932 | CYCLODEXTRIN GLUCANOTRANSFERASE - The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from | 2017-06-15 |
20170166933 | PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product. | 2017-06-15 |
20170166934 | Processes For Producing Ethanol And Yeast - The present invention relates to processes for producing ethanol from starch-containing material by liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase; saccharifying using a glucoamylase and fermenting using a | 2017-06-15 |
20170166935 | MICROORGANISM STRAINS FOR THE PRODUCTION OF 2.3- BUTANEDIOL - A recombinant yeast having a reduced pyruvate decarboxylase activity, in the génome of which has been inserted: —one or more nucleic acids encoding an acetolactate synthase or ALS, —one or more nucleic acids encoding an acetolactate decarboxylase or ALD, —one or more nucleic acids encoding a butancdiol dehydrogenase or BDH, and —one or more copies of a nucleic acids encoding a NADH oxidase or NOXE. | 2017-06-15 |
20170166936 | PROCESSES FOR THE PRODUCTION OF HYDROXYCINNAMIC ACIDS USING POLYPEPTIDES HAVING TYROSINE AMMONIA LYASE ACTIVITY - The present invention generally relates to the field of biotechnology as it applies to the production of hydroxycinnamic acids using polypeptides having tyrosine ammonia lyase activity. More particularly, the present invention pertains to polypeptides having tyrosine ammonia lyase activity and high substrate specificity towards tyrosine, which makes them particularly suitable in the production of p-coumaric acid and other hydroxycinnamic acids. The present invention thus provides processes for the production of p-coumaric acid and other hydroxycinnamic acids employing these polypeptides as well as recombinant host cells expressing same. | 2017-06-15 |
20170166937 | MODIFIED MICROORGANISM FOR IMPROVED PRODUCTION OF FINE CHEMICALS ON SUCROSE - The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms. | 2017-06-15 |
20170166938 | PRODUCTION AND COMPOSITION OF FRUCTOSE SYRUP - Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose. | 2017-06-15 |
20170166939 | Enzyme Compositions and Uses Thereof - The present invention relates to enzyme compositions and methods of producing and using the compositions for the saccharification of lignocellulosic material. | 2017-06-15 |
20170166940 | METHOD OF PRETREATMENT FOR ENHANCED ENZYMATIC HYDROLYSIS - The present invention discloses a method for producing readily available and hydrolysable polysaccharide-enriched biomass from lignocellulosic biomass while quantitatively retaining carbohydrate and producing fewer amounts of inhibitors. The method includes treating lignocellulosic biomass with an aqueous solution at elevated temperatures in a sequential step using hydrazine hydrate and sodium sulphite. In accordance with the present invention, the biomass may be further treated with a saccharification enzyme consortium to produce fermentable sugars. | 2017-06-15 |
20170166941 | METHOD FOR MODULATING GALACTOSYLATION OF RECOMBINANT PROTEIN THROUGH OPTIMIZATION OF CULTURE MEDIUM - Disclosed are a method for preparing a target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including increasing the osmolality of a culture solution of animal cells which express a target recombinant protein during the animal cell culture; a method for preparing a target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including supplementing asparagine to a culture solution of animal cells which express a target recombinant protein during the animal cell culture; a method for preparing target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including increasing the osmolality of an animal cell culture which expresses a target recombinant protein and supplementing asparagine thereto during the animal cell culture; and a target recombinant protein with modulated galactosylation, which is prepared by the method. | 2017-06-15 |
20170166942 | OVEREXPRESSION OF N-GLYCOSYLATION PATHWAY REGULATORS TO MODULATE GLYCOSYLATION OF RECOMBINANT PROTEINS - Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway. | 2017-06-15 |
20170166943 | OLIGOSACCHARIDE MODIFICATION AND LABELING OF PROTEINS - The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention. | 2017-06-15 |
20170166944 | ISOLATED POLYNUCLEOTIDE INCLUDING PROMOTER REGION, HOST CELL INCLUDING THE SAME, AND METHOD OF EXPRESSING TARGET GENE USING THE HOST CELL - Provided is a recombinant polynucleotide including a promoter region derived from an acetic acid-producing bacterium and a polynucleotide sequence encoding a target protein operably linked to the promoter, a host cell including the same, and a method of expressing a target gene or protein using the host cell. | 2017-06-15 |
20170166945 | TRANSGENIC BACTERIA WITH EXPANDED AMINO ACID USAGE AND NUCLEIC ACID MOLECULES FOR USE IN THE SAME - Recombinant bacterial cells are provided that comprise a stable non-canonical amino acid translation pathway. In some aspects, the bacteria comprise nucleic acids encoding a non-canonical amino acid translation pathway (e.g., a tRNA for incorporation of a non-canonical amino acid, such selenocysteine); a marker polypeptide that includes the non-canonical amino acid. Recombinant tRNA and selection marker coding sequences are likewise provided. | 2017-06-15 |
20170166946 | ELECTROCHEMICAL BIOSENSOR USING DUAL ELECTRODE PAIR - An electrochemical biosensor using a sensing system includes a working electrode including an active surface modified through a linker; and an auxiliary electrode. The sensor has a high current value compared with an existing sensor and retains excellent stability and sensitivity, and thus can be expected to be easily used for sensing various kinds of biomaterials. | 2017-06-15 |
20170166947 | Methods for the Determination of Biogenic Gas - Provided are methods of estimating a volume of a biogenic gas for an area of interest. The method includes predicting a methanogenesis rate for one or more of the periods of time for the area of interest based on energy available for microbial activity and calculating the volume of the biogenic gas based on the predicted methanogenesis rate for the one or more of the periods of time for the area of interest. | 2017-06-15 |
20170166948 | CELL CULTURE EVALUATION SYSTEM AND METHOD - A cell culture evaluation system includes a cell culture device 10 culturing a plurality of cell culture units of which arrangements are changed; and a cell quality evaluation device 60 including a cell quality evaluation portion 61 that performs image-evaluation on qualities based on images of the cell culture units and a cell arrangement-position storage unit 64 that stores respective arrangement-positions of the cell culture units before and after the arrangement change. When portion of the cell culture units among the plurality of cell culture units are selected to be subjected to image-evaluation, if cell culture units of which results of the image-evaluation do not satisfy predetermined conditions exist, cell culture units, which have been arranged in a predetermined distance range from the cell culture units that do not satisfy the conditions, are specified and evaluated to a point of time of the image-evaluation based on the stored respective arrangement-positions. | 2017-06-15 |
20170166949 | METHODS AND SYSTEMS FOR PREDICTING DRUG-RESPONSE - Disclosed herein are methods and systems for determining whether a cell is resistant to one or more drugs. Also, disclosed herein are methods and systems for monitoring the treatment of a cancer patient to determine whether the cancerous cells being treated are resistant to the treatment. Further, disclosed herein are methods and systems for predicting the responsiveness of a cell to a drug. Also, disclosed herein are methods and systems to determine the rate of the efficacy of a chemotherapeutic drug on a cancerous, neoplastic or damaged cells | 2017-06-15 |
20170166950 | COMPOSITIONS AND METHODS FOR IDENTIFYING ENZYME MODULATORS OR INHIBITORS - The invention is directed to compositions to screen for compounds, e.g., small molecules or drugs, that can modulate or inhibit enzymes, e.g., proteases, such as viral proteases, e.g., HIV proteases; and methods for making and using these compositions. In alternative embodiment, the invention provides compositions and methods for identifying compositions, e.g., drug molecules that can modulate or inhibit enzymes, e.g., proteases, proteinases or peptidases or the like, e.g., HIV proteases. In alternative embodiments, the invention provides cell-based assays to screen for compositions, e.g., small molecules or drugs, that modulate or inhibit or modify the activity of enzymes such as proteases, proteinases or peptidases or the like, such as calcium-dependent protein convertases involved in HIV envelop protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In alternative embodiment, the compositions and methods of the invention are adapted for high through-put or multiplexed screening of compounds, e.g., drug molecules that can modulate or inhibit enzymes. | 2017-06-15 |
20170166951 | METHOD FOR DESIGNING PROBE IN DNA MICROARRAY, AND DNA MICROARRAY PROVIDED WITH PROBE DESIGNED THEREBY - Provided is a probe to be used in a DNA microarray having an excellent detection rate of a polymorphism such as SNP contained in genomic DNA. A method for designing a probe according to the invention includes the steps of: specifying one or more regions covering at least a part of fragments flanked by restriction enzyme recognition sites recognized by a restriction enzyme, contained in genomic DNA derived from an organism to be tested; and designing a probe for the specified one or more regions for detecting the fragment in the organism to be tested. | 2017-06-15 |
20170166952 | Polymer-Based Signal Amplification for Protein and Cell Detection - The present invention relates generally to compositions, methods and kits for detection of a molecule of interest. The present invention is based, at least in part, on the design of nucleic acid oligonucleotides such that they can be used as molecular building blocks which can be assembled (polymerized) to form a dsDNA polymerization product which can be detected and further, can be disassembled (depolymerized) such that the polymerization product is no longer detectable. The invention can be performed multiple times on the same cell population, and is therefore useful for highly sensitive in situ detection of multiple biomarkers. | 2017-06-15 |
20170166953 | METHODS AND KITS FOR JOINING FRAGMENTED NUCLEIC ACIDS TOGETHER - Methods and kits for joining fragmented nucleic acid sequences together are provided, including performing an amplifying step including contacting a sample suspected of including a fragmented target nucleic acid with a pair of external primers and a pair of self-complementary internal primers, and generating a full length target nucleic acid. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, kits are provided that are designed for the detection of a target nucleic acid sequence. | 2017-06-15 |
20170166954 | METHODS FOR DETECTING NUCLEIC ACID FRAGMENTS - Provided are methods for detecting and analyzing polynucleotides in a biological sample or sample derived therefrom for example, using a synthetic template polynucleotide. In some aspects, a target polynucleotide in the sample hybridizes to the template polynucleotide and is extended by a polymerase, generating an extended target polynucleotide. In some examples, the extended target polynucleotide is amplified, for example, by polymerase chain reaction, and sequences of the target polynucleotide determined, for example, by priming in the region of the extended target polynucleotide generated by extension and sequencing towards the region having identity to the target polynucleotide. In some aspects, the target polynucleotide is thereby detected in the sample and its sequence identified. In some aspects, the provided methods can be used to capture polynucleotide fragments in a biological sample, for example, plasma, and determine respective biomarkers they carry, for example, for cancer diagnosis and prognosis. | 2017-06-15 |
20170166955 | Composition and Method for Stabilizing Nucleic Acids in Biological Samples - Methods, compositions, and kits for stabilizing both human and microbial deoxyribonucleic acid (DNA) present in complex biological samples, such as feces, are disclosed. In particular, aqueous compositions for stabilizing DNA contained in biological samples at ambient temperature are disclosed, together with associated methods and kits using same. In one aspect, the compositions comprise a chelating agent present at a concentration of at least about 150 mM, and the composition has a pH of at least about 9.5. | 2017-06-15 |
20170166956 | Methods for DNA Preparation for Multiplex High Throughput Targeted Sequencing - Disclosed are methods for parallel single-step DNA purification starting with multiple crude biological samples for subsequent parallel PCR amplification of target DNA that attaches a unique DNA sequence tag (barcode) allowing all parallel processed samples to be combined into a single high-throughput sequencing run. The methods disclosed herein can be used to prepare and sequence dozens or hundreds of targeted samples as part of a rapid, highly parallel process, after which individual sample sequencing results are separated using the sample-specific tags (barcodes) to obtain results for each sample. | 2017-06-15 |
20170166957 | AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS - System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected. | 2017-06-15 |
20170166958 | ENDORIBONUCLEASE COMPOSITIONS AND METHODS OF USE THEREOF - The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell. | 2017-06-15 |
20170166959 | Method For Analyzing Nucleic Acid Derived From Single Cell - The present invention relates to a method of analyzing a composition of nucleic acids derived from a single cell using a microplate including a plurality of reaction wells, the microplate having one bead arranged in one reaction well, the one bead having bound thereto a plurality of molecules of single-stranded oligonucleotides, the single-stranded oligonucleotides each having a nucleic acid capture sequence exposed at the 3′ end and a barcode sequence on the 5′ side of the nucleic acid capture sequence, the barcode sequence including a base sequence that differs from bead to bead. | 2017-06-15 |