26th week of 2016 patent applcation highlights part 28 |
Patent application number | Title | Published |
20160186156 | ARTIFICIAL CELLULOSOMES COMPRISING MULTIPLE SCAFFOLDS AND USES THEREOF IN BIOMASS DEGRADATION - Multi-enzyme complexes comprising an array of scaffold subunits designed for efficient integration of a plurality of carbohydrate-active enzymes are provided. | 2016-06-30 |
20160186157 | INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATION - A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. | 2016-06-30 |
20160186158 | VACCINES COMPRISING LEISHMANIA POLYPEPTIDES FOR THE TREATMENT AND DIAGNOSIS OF LEISHMANIASIS - Compositions and methods for preventing, treating and detecting leishmaniasis are disclosed. The compositions generally comprise polypeptides comprising | 2016-06-30 |
20160186159 | SOLID TEXTILE DETERGENT WITH IMPROVED PROTEASE PERFORMANCE - The aim of the invention is to improve the detergent power of solid textile detergents having a pH value ranging from 4 to under 10 in a solution containing 1 wt. % in demineralized water at 20° C. Said aim is achieved by using a protease which comprises an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in SEQ ID NO. 1 and contains, at position 99 of SEQ ID NO. 1, the amino acid glutamic acid (E) or aspartic acid (D), or the amino acid asparagine (N) or glutamine (Q), or the amino acid alanine (A) or glycine (G) or serine (S). | 2016-06-30 |
20160186160 | ASPARTIC PROTEASES - The invention relates to aspartic proteases, and particularly to aspartic proteases for plants. Disclosed are modified plant aspartic proteases, and methods for their manufacture, and uses thereof. Particularly contemplated are the uses of aspartic proteases in inducing skin desquamation. | 2016-06-30 |
20160186161 | Alkenol dehydratase variants - Described are alkenol dehydratase variants having improved activity in catalyzing the conversion of a compound corresponding to the general formula C | 2016-06-30 |
20160186162 | ALDOLASE, ALDOLASE MUTANT, AND METHOD AND COMPOSITION FOR PRODUCING TAGATOSE BY USING SAME - This disclosure relates to aldolase, an aldolase mutant, and a method and a composition for producing tagatose by using the same. The feature of the disclosure is environment-friendly due to the use of an enzyme acquired from microorganisms, requires only a simple process of enzyme immobilization, uses a low-cost substrate in a substrate compared with a conventional method for producing tagatose and has a remarkably high yield, thereby greatly reducing production costs and maximizing production effects. | 2016-06-30 |
20160186163 | ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL OVERPRODUCTION FROM CELLULOSIC MATERIALS - The invention relates to engineering microbial cells for utilization of cellulosic materials as a carbon source, including xylose. | 2016-06-30 |
20160186164 | THERMOSTABLE BLUNT-END LIGASE AND METHODS OF USE - Thermostable blunt-end ligases suitable for use in nucleic acid ligation reactions at elevated temperatures are provided. The ligases comprise fusion proteins including a DNA ligase and a DNA binding protein, e.g., a T4 DNA ligase with an N-terminal p50 fusion. The fusion proteins may include peptide linkers, peptide mimetics, terminal additions, tag peptides, D-amino acids, sugars, non-amino acid organic moieties, and polymers. The ligases are suitable for use in ligation reactions, including uniform-temperature ligation reactions, performed at about 60° C. or higher, e.g., at about 75° C. The ligases are suitable for use in nucleic acid amplification schemes with temperature cycling, e.g., temperature cycles to about 60° C. or higher, or temperature cycles from about 94° C. to about 60° C. Such nucleic acid amplification schemes may include one, two, three, or more temperature cycles. Methods of using the ligases, and articles of manufacture comprising the ligases are provided. | 2016-06-30 |
20160186165 | Multisort cell separation method - The invention is directed to a method for enriching target cells from a sample of cells characterized by:
| 2016-06-30 |
20160186166 | HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS - The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes). | 2016-06-30 |
20160186167 | Method and Device for Processing a Sample of Biological Material Containing Target Cells and Companion Cells in Order to Extract Nucleic Acids of the Target Cells - A method for processing a sample of biological material containing target cells and companion cells in order to extract nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the companion cells from the sample. The method additionally includes decomposing the target cells by chemical or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method further includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acid of the target cells. | 2016-06-30 |
20160186168 | PROCESSES AND HOST CELLS FOR GENOME, PATHWAY, AND BIOMOLECULAR ENGINEERING - The present disclosure provides compositions and methods for genomic engineering. | 2016-06-30 |
20160186169 | Effective method for specific gene silencing using artificial small RNA - A nucleic acid molecule comprising a stem-loop structure, a nucleic acid complex comprising the nucleic acid molecule, a composition for delivering a target recognition sequence, and the composition comprising the nucleic acid complex. An artificial small ribonucleic acid of the stem-loop structure stably maintains single-strandedness of a target recognition sequence which interacts with a nucleic acid of interest for a gene of interest of a prokaryote, thereby providing a nucleic acid complex for effective silencing of the gene of interest. | 2016-06-30 |
20160186170 | COMPOSITIONS AND METHODS FOR INDUCED TISSUE REGENERATION IN MAMMALIAN SPECIES - Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. Said methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration. | 2016-06-30 |
20160186171 | AGENTS AND METHODS FOR INHIBITING MIR-148A FOR THE MODULATION OF CHOLESTEROL LEVELS - Elevated blood levels of low-density lipoprotein-cholesterol (LDL-C, or “bad” cholesterol) are strongly linked to circulatory disorders, e.g. cardiovascular disease such as atherosclerosis, angina, coronary heart disease, heart attack, stroke, etc. The LDL receptor (LDLR) mediates uptake of LDL-C (low density lipoprotein-cholesterol) by, e.g. hepatic cells. As described herein, miR-148a regulates LDLR expression in human hepatic cells. Accordingly, described herein are methods and compositions relating to, e.g. regulating cholesterol levels by modulating the level of miR-148a. | 2016-06-30 |
20160186172 | Compositions and methods for inhibiting hepcidin antimicrobial peptide (HAMP) or HAMP-related gene expression - The invention relates to lipid formulated double-stranded ribonucleic acid (dsRNA) targeting a hepcidin antimicrobial peptide (HAMP) and/or HAMP-related gene, and methods of using the dsRNA to inhibit expression of HAMP and/or HAMP-related genes. | 2016-06-30 |
20160186173 | C-MYC ANTISENSE OLIGONUCLEOTIDES AND METHODS FOR USING THE SAME TO TREAT CELL-PROLIFERATIVE DISORDERS - Provided herein are antisense oligonucleotides that can effectively prevent or decrease c-myc protein expression as well as decrease overall rates of cell proliferation in in vitro and mammalian in vivo models of cell proliferative disorders as well as methods for using the same. | 2016-06-30 |
20160186174 | SUBSTITUTED MORPHOLINO COMPOUNDS ANALOGS THEREOF AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM - The present invention provides substituted morpholino compounds, analogs thereof and oligomeric compounds prepared therefrom. More particularly, incorporation of one or more of the substituted morpholino compounds into an oligomeric compound is expected to enhance one or more properties of the oligomeric compound. Such oligomeric compounds can also be included in a double stranded composition. In certain embodiments, the oligomeric compounds provided herein are expected to hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. | 2016-06-30 |
20160186175 | BICYCLIC MORPHOLINO COMPOUNDS AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM - The present invention provides bicyclic morpholino compounds and oligomeric compounds prepared therefrom. More particularly, incorporation of one or more of the bicyclic morpholino compounds into an oligomeric compound is expected to enhance one or more properties of the oligomeric compound. Such oligomeric compounds can also be included in a double stranded composition. In certain embodiments, the oligomeric compounds provided herein are expected to hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. | 2016-06-30 |
20160186176 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF BETA-CATENIN BY DOUBLE-STRANDED RNA - This invention relates to compounds, compositions, and methods useful for reducing β-catenin target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents. | 2016-06-30 |
20160186177 | Methods for Diagnosing Lung Cancer Using MicroRNAs - Described herein are methods and compositions for the diagnosis and treatment of lung cancers. Methods of identifying inhibitors of tumorigenesis are also provided. | 2016-06-30 |
20160186178 | SPHERICAL NUCLEIC ACID-BASED CONSTRUCTS AS IMMUNOSTIMULATORY AGENTS FOR PROPHYLACTIC AND THERAPEUTIC USE - Aspects of the invention relate to spherical nucleic acid-based constructs and related methods and compositions thereof. The compositions of the invention are useful for activating agonists of nucleic acid interacting complexes, such as TLRs, stimulating an immune response, and treating diseases such as infectious disease, cancer, allergies, allergic diseases, and autoimmune disease | 2016-06-30 |
20160186179 | Methods and Compositions for Modulating Gene Expression Using Oligonucleotide Based Drugs Administered in Vivo or in Vitro - Compositions and method for down modulating target gene expression which RNA interference, as well as methods for administering said compositions are disclosed. The method comprises administering a first oligonucleotide strand to a cell, incubating the cells for a time period suitable for uptake of the first oligo nucleotide strand prior to administration of a second oligonucleotide strand, wherein the first strand and the second strand form an intracellular duplex which is effective to catalyze degradation of gene target mRNA or inhibit translation of said mRNA. | 2016-06-30 |
20160186180 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2016-06-30 |
20160186181 | COMPOSITION FOR REDUCING SENESCENCE OF CELL OR SUBJECT COMPRISING SMURF2 INHIBITOR AND USE THEREOF - A composition for reducing a level of senescence of a cell or subject, a method of reducing a level of senescence in a cell or subject by using the composition, and a method of preventing and treating symptoms or diseases related to or caused by senescence of a cell or subject. | 2016-06-30 |
20160186182 | RNA INTERFERENCE COMPOSITIONS AND METHODS FOR MALIGNANT TUMORS - This invention provides compositions for use in distributing active agents for treating a malignant tumor in a subject. The compositions contain RNAi molecules targeted to a human GST-π, along with RNAi molecules targeted to a human p21, and a pharmaceutically acceptable carrier. The carrier can include nanoparticles composed of an ionizable lipid, a structural lipid, one or more stabilizer lipids, and a lipid for reducing immunogenicity of the nanoparticles. This invention further provides methods for preventing or treating a malignant tumor by administering a therapeutically effective amount of an RNAi composition. | 2016-06-30 |
20160186183 | METHODS AND COMPOSITIONS FOR TREATING MALIGNANT TUMORS ASSOCIATED WITH KRAS MUTATION - This invention provides methods and compositions for preventing, treating or ameliorating one or more symptoms of a malignant tumor associated with KRAS mutation in a mammal in need thereof, by identifying a tumor cell in the mammal, the tumor cell comprising at least one of: (i) a mutation of the KRAS gene, and (ii) an aberrant expression level of KRAS protein; and administering to the mammal a therapeutically effective amount of a composition comprising one or more RNAi molecules that are active in reducing expression of GST-π. | 2016-06-30 |
20160186184 | Methods and Compositions for the Treatment of Prostate Related Disorders using miR-1 - Methods and compositions for the treatment of prostate associated disorders are disclosed. | 2016-06-30 |
20160186185 | MODIFIED NUCLEOSIDES, ANALOGS THEREOF AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM - The present invention provides modified nucleosides, analogs thereof and oligomeric compounds prepared therefrom. More particularly, the present invention provides modified nucleosides and analogs thereof that are useful for incorporation at the terminus of an oligomeric compound. Such oligomeric compounds can also be included in a double stranded composition. In some embodiments, the oligomeric compounds provided herein are expected to hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. | 2016-06-30 |
20160186186 | Fermentive Production of Four Carbon Alcohols - Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. | 2016-06-30 |
20160186187 | REGULATABLE GENE EXPRESSION - The present invention relates to a regulatable gene expression construct comprising a nucleic acid molecule comprising two or more regulation sequences encoding respective RNA molecules comprising a riboswitch responsive to an effector compound, said riboswitch being operably linked to a respective coding region which encodes a respective modulator compound for modulating the action of a respective growth regulator compound and each said riboswitch in each regulation sequence being selected be responsive to the same effector compound to trigger expression of its respective modulator compound. The invention also relates to a method of using the regulatable gene expression construct for selecting from a metagenomic library a primary modulator compound which effects a chemical transformation of a substrate into said effector compound or transports said effector compound into a micro-organism comprising the regulatable gene expression construct. | 2016-06-30 |
20160186188 | METHODS FOR ALTERING POLYPEPTIDE EXPRESSION AND SOLUBILITY - The invention is directed to methods and metric suitable for use in determining the solubility, expression and usability of a polypeptide encoded by a nucleic acid sequence. In certain aspects, the invention also relates to methods for introducing modifications in a polypeptide, for example through substitution of one or more codons in the nucleic acid sequence encoding the polypeptide, to increase or decrease the solubility, expression or usability of the polypeptide. | 2016-06-30 |
20160186189 | SITE-SPECIFIC INCORPORATION OF PHOSPHOSERINE INTO PROTEINS IN ESCHERICHIA COLI - Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNA | 2016-06-30 |
20160186190 | EXPRESSION VECTOR FOR PRODUCTION OF RECOMBINANT PROTEINS IN PROKARYOTIC HOST CELLS - An expression vector for production of a recombinant protein in a host cell is provided. The expression vector includes a nucleotide sequence of Sequence ID No 2 encoding for a leader peptide of sequence ID No 3. | 2016-06-30 |
20160186191 | Tailored Oils Produced from Recombinant Heterotrophic Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 2016-06-30 |
20160186192 | GENE EXPRESSION TECHNIQUE - The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising:
| 2016-06-30 |
20160186193 | Plastid Transformation of Maize - A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs. | 2016-06-30 |
20160186194 | TEMPORAL REGULATION OF GENE EXPRESSION BY MICRORNAS - This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention. | 2016-06-30 |
20160186195 | MODIFICATION OF SOYBEAN SEED COMPOSITION TO ENHANCE FEED, FOOD AND OTHER INDUSTRIAL APPLICATIONS OF SOYBEAN PRODUCTS - Polynucleotide sequences encoding diacylglycerol acyltransferases are used in combination with other coding sequence to modify the composition of soybean seed. The modified seed can be used to enhance feed, food and other industrial applications of soybean products. | 2016-06-30 |
20160186196 | METHOD AND COMPOSITION FOR GENERATING PROGRAMMED CELL DEATH RESISTANT ALGAL CELLS - The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation. | 2016-06-30 |
20160186197 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES, AND METHODS OF USING SAME FOR INCREASING PLANT YIELD AND/OR AGRICULTURAL CHARACTERISTICS - Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 or 4804, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, harvest index, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant. | 2016-06-30 |
20160186198 | USE OF ALDH7 FOR IMPROVED STRESS TOLERANCE - The present invention relates to the field of plant molecular biology, more particularly to the regulation of genes that increase drought tolerance and yield. Provided herein are methods finding use in agriculture for increasing drought tolerance in dicot and monocot plants. Methods comprise modulating the aldehyde profile of a plant. Compositions comprise plants comprising modulated aldehyde profiles. A polynucleotide encoding an ALDH7 polypeptide, operably linked to a promoter that drives expression in a plant, may be introduced into a plant. Also provided are transformed plants, plant tissues, plant cells, and seeds thereof. | 2016-06-30 |
20160186199 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES FOR INCREASING PLANT YIELD AND/OR AGRICULTURAL CHARACTERISTICS - Provided are isolated polynucleotides which comprise a nucleic acid sequence at least 80% identical to SEQ ID NO: 321, 1-320, 322-480, 793-2945 or 2946; isolated polypeptides which comprise an amino acid sequence at least 80% homologous to SEQ ID NO: 517, 481-516, 518-792, 2947-4662 or 4663, nucleic acid constructs comprising same, transgenic cells and plants expressing same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant. | 2016-06-30 |
20160186200 | GLYPHOSATE RESISTANT CLASS 1 EPSPS GENES - The present invention relates to DNA molecules encoding glyphosate tolerant mutant EPSPS enzymes as well as constructs and plants comprising said enzymes. Also included are methods of using said enzymes, including use as a selectable marker, use to make transgenic plants resistant to glyphosate containing herbicides and methods of controlling weeds. | 2016-06-30 |
20160186201 | PLANTS RESISTANT TO PATHOGENIC MICROORGANISMS GROWING IN VASCULAR TISSUES - The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of | 2016-06-30 |
20160186202 | AXMI115 VARIANT INSECTICIDAL GENE AND METHODS FOR ITS USE - Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof. | 2016-06-30 |
20160186203 | GHO/SEC24B2 AND SEC24B1 NUCLEIC ACID MOLECULES TO CONTROL COLEOPTERAN AND HEMIPTERAN PESTS - This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby. | 2016-06-30 |
20160186204 | INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE - Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. | 2016-06-30 |
20160186205 | Manipulation of Self-Incompatibility in Plants - The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Poaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. The present invention also relates to expression of self-gamete recognition genes in plants and to related nucleic acids, constructs, molecular markers and methods. | 2016-06-30 |
20160186206 | OPTIMIZED EXPRESSION CASSETTE FOR EXPRESSING A POLYPEPTIDE WITH HIGH YIELD - The present invention is based on the finding that the combination of a specific 5′UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield. | 2016-06-30 |
20160186207 | NOVEL METHOD - The invention relates to a method of enhancing the potency of a cell (for example, to a totipotent state), by introducing a TET family gene, derivative or fragment thereof into the cell. The invention also relates to methods and kits for preparing cells with enhanced potency, and uses of said cells. | 2016-06-30 |
20160186208 | Methods of Mutating, Modifying or Modulating Nucleic Acid in a Cell or Nonhuman Mammal - The invention is directed to a method of mutating one or more target nucleic acid sequences in a stem cell or a zygote comprising introducing into the stem cell or zygote (i) ribonucleic acid (RNA) sequences that comprise a portion that is complementary to a portion of each of the target nucleic acid sequences and comprise a binding site for a CRISPR associated (Cas) protein; and a Cas nucleic acid sequence or a variant thereof that encodes a Cas protein having nuclease activity. The stem cell or zygote is maintained under conditions in which the target nucleic acid sequences are mutated in the stem cell or zygote. The invention is also directed to methods of producing a non human mammal carrying mutations and methods of modulating the expression and/or activity target nucleic acid sequences and cells or zygotes. | 2016-06-30 |
20160186209 | RECOMBINANT CELLS AND METHODS OF USING SUCH CELLS TO IDENTIFY CIRCADIAN RHYTHM MODULATORS - The invention provides recombinant cells comprising detectable reporters useful in identifying agents, genes, and other modulators of circadian period length and amplitude. Such modulators are useful for resetting the circadian clock in a variety of contexts (e.g., jet lag, shift work). Such cells are also useful in selecting an administration regimen for a therapeutic agent, where the agent's efficacy and/or adverse side effects show circadian effects. | 2016-06-30 |
20160186210 | COMPOSITIONS AND METHODS FOR IDENTIFYING LATENTLY INFECTED CELLS - The present disclosure provides for recombinant nucleic acids, and cells and virions comprising the recombinant nucleic acids, that can be used to identify, isolate, and/or purify cells latently infected with immunodeficiency virus. A subject recombinant nucleic acid includes (a) a first nucleotide sequence encoding a first reporter polypeptide that produces a first detectable signal, where the first nucleotide sequence is operably linked to an immunodeficiency virus promoter and is translated as an early gene; and (b) a second nucleotide sequence encoding a second reporter polypeptide that produces a second detectable signal that is distinguishable from the first detectable signal, where the second nucleotide sequence is operably linked to a non-immunodeficiency virus promoter. In some aspects, the first and second nucleotide sequences are both positioned between a shared 5′ long terminal repeat (LTR) and a shared 3′ LTR. Also provided are related methods. | 2016-06-30 |
20160186211 | RAAV-BASED COMPOSITIONS AND METHODS FOR TREATING ALPHA-1 ANTI-TRYPSIN DEFICIENCIES - The invention relates to isolated nucleic acids and rAAV-based compositions, methods and kits useful for treating genetic diseases (e.g., alpha-1 antitrypsin deficiency). | 2016-06-30 |
20160186212 | MODIFIED CELL BY PUTTING MATERIAL INTO THE CELL WITHOUT USING DELIVERY VEHICLE - The present invention relates to a modified cell prepared by putting material into the cell without using delivery vehicle and to a cell composition containing the modified cell. | 2016-06-30 |
20160186213 | DELIVERY, ENGINEERING AND OPTIMIZATION OF TANDEM GUIDE SYSTEMS, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION - The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity. | 2016-06-30 |
20160186214 | MODIFIED CASCADE RIBONUCLEOPROTEINS AND USES THEREOF - A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence(Cascade); the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression. A pair of ribonucleotides fused to FokI dimers may be used to generate double-strand breakages in the DNA to facilitate these applications in a sequence-specific manner. | 2016-06-30 |
20160186215 | Economic Ethanol Fermentation Sugar Stream, Processes and Systems of Producing Same - Methods and system for producing a slip stream of sugar, for example for use in the production of one or more chemicals, in ethanol fermentation facilities. In some embodiments, the methods and systems have little to no impact on the level of production of ethanol, despite also producing a slip stream of sugar. The methods and systems can be implemented in dry mill ethanol, wet mill ethanol, and lignocellulosic ethanol fermentation facilities and processes. | 2016-06-30 |
20160186216 | RECOMBINANT STRAIN FOR PRODUCING 2,3-BUTANEDIOL, COMPRISING (A) INACTIVATED LACTATE DEHYDROGENASE AND (B) INACTIVATED SUCROSE REGULATOR - The present invention relates to a recombinant strain for producing 2,3-butanediol, comprising (a) an inactivated lactate dehydrogenase and (b) an inactivated sucrose regulator. According to the present invention, it is possible to economically produce 2,3-butanediol using a cheap carbon source, and the efficiency and productivity of 2,3-butanediol is remarkable compared with a wild type. | 2016-06-30 |
20160186217 | METHOD FOR BIOCATALYTIC SYNTHESIS OF SUBSTITUTED OR UNSUBSTITUTED PHENYLACETIC ACIDS AND KETONES HAVING ENZYMES OF MICROBIAL STYRENE DEGRADATION - The present invention relates to a method for the biocatalytic synthesis of substituted and unsubstituted phenylacetic acids and ketones from styrenes and bicyclic aromatic hydrocarbons using enzymes of microbial styrene degradation in a whole-cell sensor, as well as a kit for the biocatalytic synthesis of substituted and unsubstituted phenylacetic acids and ketones containing a whole-cell catalyst and the use of the method, wherein the method comprises the following steps:
| 2016-06-30 |
20160186218 | METHOD AND SYSTEM FOR PRODUCTION OF HYDROGEN, METHANE, VOLATILE FATTY ACIDS, AND ALCOHOLS FROM ORGANIC MATERIAL - A method for producing H | 2016-06-30 |
20160186219 | Tailored Oils Produced from Recombinant Oleaginous Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 2016-06-30 |
20160186220 | PROCESS FOR PREPARING (METH)ACRYLIC ESTERS OF FUNCTIONALIZED FURYL ALCOHOLS - The invention relates to a process for preparing a compound of the formula (I), | 2016-06-30 |
20160186221 | COMPOSITIONS AND METHODS OF USE - The present compositions and methods relate to a beta-mannanase from | 2016-06-30 |
20160186222 | FUNGAL XYLANASES AND XYLOSIDASES - The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are | 2016-06-30 |
20160186223 | PRODUCTION OF OLIGOSACCHARIDES - The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide. | 2016-06-30 |
20160186224 | ENHANCED LIGATION REACTIONS - In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends. | 2016-06-30 |
20160186225 | METHODS FOR IMPROVED PRODUCTION OF REBAUDIOSIDE D AND REBAUDIOSIDE M - Methods for recombinant production of steviol glycoside and compositions containing steviol glycosides are provided by this invention. | 2016-06-30 |
20160186226 | METHOD FOR PRODUCING EXTRACELLULAR PROTEINS FROM GENUS TEPIDIMONAS - The disclosure provides a method for producing extracellular proteins from genus | 2016-06-30 |
20160186227 | METHODS AND COMPOSITIONS FOR PREVENTING NORLEUCINE MISINCORPORATION INTO PROTEINS - The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein. | 2016-06-30 |
20160186228 | METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN - Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture. | 2016-06-30 |
20160186229 | METHODS AND APPARATUS FOR DETERMINING ANALYTE IN A SAMPLE USING A SENSOR HAVING ELECTRODES WHICH ARE PROVIDED WITH AN ENZYME AND A MEDIATOR - The invention relates to a method of and apparatus for determining concentration of an analyte, such as glucose, in a fluid sample, such as body fluid or control solution, using a mediated redox reaction. In particular, the method relates to mitigation of the effects of haematocrit on the response of sensor device used in such a method or apparatus. The invention describes a method of determining concentration of an analyte in a fluid sample deposited on a sensor device having a working electrode and a counter electrode, in which the electrodes are provided with an enzyme and a mediator for carrying out a mediated redox reaction, the method comprising: applying a first oxidising potential between the working and counter electrodes during a first time period; applying a second reducing potential between the working and counter electrodes during a second time period; determining a reaction parameter, the reaction parameter being indicative of the concentration of reduced mediator at the counter electrode after commencement of the second time period; using the reaction parameter to determine the concentration of analyte. | 2016-06-30 |
20160186230 | Enzyme Electrode - The present invention provides an enzyme electrode comprising: an electrode; and a detection layer which is in contact with the electrode, where the detection layer includes an enzyme, a crosslinking agent, an electrically conductive polymer and a sugar, wherein electrons are transferred between the enzyme in the detection layer and the electrode. | 2016-06-30 |
20160186231 | USE OF A FLUIDIC DEVICE - A fluidic device ( | 2016-06-30 |
20160186232 | MODIFIED AMADORIASE AND METHOD FOR PRODUCING THE SAME, AGENT FOR IMPROVING SURFACTANT RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING THE SAME - Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus | 2016-06-30 |
20160186233 | SCREENING METHODS USING FYN IN COMBINATION WITH TAU PROTEIN - The present invention provides materials and methods relating to screening for compounds useful in the treatment of Alzheimer's disease and related conditions. In particular, screening methods using tyrosine kinases are provided, as are methods relating to the role of tyrosine kinases as therapeutic targets. | 2016-06-30 |
20160186234 | CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF - There has been a demand for a codon-optimized gene for the mutated catalytic domain of | 2016-06-30 |
20160186235 | APPARATUS FOR HIGH THROUGHPUT CHEMICAL REACTIONS - Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run. The subject apparatus, systems, and chips are particularly suited for performing polymerase chain reactions on thousands of nucleic acid sequences, up to and including sequences of an entire genome of an organism of interest. | 2016-06-30 |
20160186236 | REAL TIME QUANTITATIVE AND QUALITATIVE ANALYSIS METHOD FOR BIOSUBSTANCE - A method of quantitatively and qualitatively analyzing a biomaterial in real-time, the method comprising preparing a device for detecting a biomaterial, feeding a complex of first and second probes, a forward primer, a reverse primer, a sample comprising deoxynucleotide triphosphate, a polymerase having exonuclease activity, and a sample comprising target genes, and a reaction solution comprising a buffer into the reaction container, performing polymerase chain reaction comprising denaturation of the target genes in the sample, hybridization of the target genes, the complex, and the forward and reverse primers in the sample, and elongation of the primers through the polymerase having exonuclease activity, allowing for elongation of the second probe on the third probe by the polymerase after hybridizing the released second probe and the third probe fixed to the biochip, detecting a first fluorescence signal by the first phosphor and a second fluorescence signal by the second phosphor. | 2016-06-30 |
20160186237 | METHODS AND COMPOSITIONS TO ENABLE ENRICHMENT OF MINOR DNA ALLELES BY LIMITING DENATURATION TIME IN PCR OR SIMPLY ENABLE ENRICHMENT OF MINOR DNA ALLELES BY LIMITING THE DENATURATION TIME IN PCR - The present invention is directed to methods, compositions and reaction mixtures for conducting COLD-PCR, by controlling and varying a preferential denaturation time. | 2016-06-30 |
20160186238 | REACTIVITY-DEPENDENT AND INTERACTION-DEPENDENT PCR - Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition. | 2016-06-30 |
20160186239 | MULTIPLEXED ASSAY FOR QUANTITATING AND ASSESSING INTEGRITY OF CELL-FREE DNA IN BIOLOGICAL FLUIDS FOR CANCER DIAGNOSIS, PROGNOSIS AND SURVEILLANCE - A retrotransposable element based multiplexed qPCR assay to robustly quantitate and distinguish cell free DNA integrity and concentration in blood plasma and serum is described. The multiplexed system for characterizing cancer in humans includes a sample of serum, plasma, urine, or other biological fluid, the sample comprising cell free DNA, the cell free DNA comprising long and short retrotransposable element targets and an added internal positive control, the long and short targets being independent of each other, a distinctly labeled TaqMan probe corresponding to each target, a forward primer and a reverse primer corresponding to each target, a DNA standard for generating standard curves, a qPCR system for amplifying the targets and a qPCR data analysis system. The assay provides an accurate, minimally-invasive, rapid, high-throughput, and cost-effective method with the potential to complement or replace existing methods for detection, diagnosis, prognosis, treatment monitoring and/or surveillance of cancer, thereby improving patient outcomes. | 2016-06-30 |
20160186240 | DEVICES AND METHODS FOR MOLECULAR DIAGNOSTIC TESTING - A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing. | 2016-06-30 |
20160186241 | BUFFERS FOR THE STABLE STORAGE OF NUCLEIC ACIDS - Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide. | 2016-06-30 |
20160186242 | COMPOSITIONS AND METHODS FOR DETECTION OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS - Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of | 2016-06-30 |
20160186243 | LABELED ENZYME COMPOSITIONS, METHODS AND SYSTEMS - Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing. | 2016-06-30 |
20160186244 | Real Time Cleavage Assay - A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured. | 2016-06-30 |
20160186245 | MULTIPLEX DETECTION OF NUCLEIC ACIDS - Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described. | 2016-06-30 |
20160186246 | PROCESS FOR DETECTING OR QUANTIFYING NUCLEIC ACIDS IN A LIBRARY - This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention. | 2016-06-30 |
20160186247 | Detection of Chromosomal Inversions - A method and a kit for the identification of chromosomal inversions are described. Chromosomal inversions are difficult to detect unless they are quite large. The improved ability to detect chromosomal inversions is important to a number of medical applications, such as cancer and birth defects, as examples. Reporter species are attached to oligonucleotide strands designed such that they may hybridize to portions of only one of a pair of single-stranded sister chromatids prepared by the CO-FISH procedure, as an example. If an inversion has occurred, these marker probes will be detected on the sister chromatid at the same location as the inversion on the first chromatid. | 2016-06-30 |
20160186248 | METHOD FOR CENTRIFUGE MOUNTABLE MANIFOLD FOR PROCESSING FLUIDIC ASSAYS - The disclosure generally relates to a method and apparatus for centrifuge mountable manifold for processing fluid assays. In one embodiment, the disclosure relates to a method for automatically running an assay with a radially configured flowrotor by: introducing a sample fluid to an inlet of the flowrotor; rotating the flowrotor in a first direction to provide a first radial acceleration to move the sample fluid from the inlet to a first reaction chamber, the first reaction chamber having one or more reactants; retaining the assay at the first reaction chamber for a first duration by maintain rotation at a first angular velocity to induce a reaction with the reactant and to provide a reacted assay; rapidly accelerating the flowrotor at a negative angular acceleration relative to the first angular velocity until the angular velocity is reversed thereby moving the reacted assay from the first reaction chamber to a second reaction chamber having an inlet and an outlet and containing a second reactant or reactants and retaining the assay at the second chamber for a second duration by continuing the second angular velocity to thereby induce a reaction with the second reactant. | 2016-06-30 |
20160186249 | TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS - The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results. | 2016-06-30 |
20160186250 | MULTIPLEX QUANTITATIVE PCR - Disclosed are methods and compositions for determining the average length or abundance of a first target nucleic by calculating the abundance of a first target nucleic acid (T) relative to the average abundance (S) of a second and a third target nucleic acid, in a single well using a separate detection label for each target nucleic acid. In various aspects, the first target nucleic acid is a telomere. In exemplary aspects, the disclosed methods and compositions can be used to determine the average telomere length in a biological sample. The average telomere length determined using the disclosed methods and compositions can be correlated to a variety of clinically important conditions and indices. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 2016-06-30 |
20160186251 | Modified Primers for Nucleic Acid Amplification and Detection - A method of nucleic acid amplification involving using a first modified primer which provides protection to the amplification product from exonuclease degradation and a second primer. The method provides a double stranded nucleic acid, one strand of which is degraded by a double strand nucleic acid specific exonuclease to form a single stranded nucleic acid, which is protected from exonuclease degradation. | 2016-06-30 |
20160186252 | PH MEASUREMENT FOR SEQUENCING OF DNA - The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dNTP) in the presence of appropriate reagents (DNA polymerase, and polymerase reaction buffer). Alternatively, or in addition, a change in pH resulting from the incorporation of nucleotides in the DNA polymerase reaction is measured. A device is provided having delivery channels for appropriate reagents, including dNTPs, which may be delivered sequentially or in a mixture. Preferably, the dNTPs are added in a predetermined sequence, and the dNTP is incorporated or not depending on the template sequence. | 2016-06-30 |
20160186253 | NON-INVASIVE PRENATAL DIAGNOSIS OF FETAL GENETIC CONDITION USING CELLULAR DNA AND CELL FREE DNA - Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly. | 2016-06-30 |
20160186254 | Sequencing Biopolymers - The invention relates to a method and a corresponding arrangement for sequencing at least two biopolymers ( | 2016-06-30 |
20160186255 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 2016-06-30 |