27th week of 2022 patent applcation highlights part 25 |
Patent application number | Title | Published |
20220213369 | FLUORINATED ALKENE SYSTEMS - A process for transferring heat including providing an article and contacting the article with a heat transfer media. The heat transfer media includes a composition formed by the process of, contacting a compound of formula (1), R | 2022-07-07 |
20220213370 | THERMAL CONDUCTIVE SILICONE COMPOSITION, SEMICONDUCTOR DEVICE, AND METHOD FOR MANUFACTURING THE SAME - A thermal conductive silicone composition contains, in specific amounts, each of (A) an organopolysiloxane containing two or more alkenyl groups bonded to a silicon atom in one molecule, (B) an organohydrogenpolysiloxane containing two or more hydrogen atoms bonded to a silicon atom in one molecule, (C) a catalyst for hydrosilylation reaction, (D) a silver powder with a tapped density of 3.0 g/cm | 2022-07-07 |
20220213371 | COMPOSITION FOR FORMING THERMALLY CONDUCTIVE MATERIAL, THERMALLY CONDUCTIVE MATERIAL, THERMALLY CONDUCTIVE SHEET, AND DEVICE WITH THERMALLY CONDUCTIVE LAYER - An object of the present invention is to provide a composition for forming a thermally conductive material, from which a thermally conductive material having excellent thermally conductive properties and adhesiveness can be obtained. In addition, another object of the present invention is to provide a thermally conductive material, a thermally conductive sheet, and a device with a thermally conductive layer. A composition for forming a thermally conductive material of the present invention contains a thermosetting compound A, boron nitride particles B containing boron nitride and having an average particle diameter of 25.0 μm or more, and boron nitride particles C containing boron nitride and having an average particle diameter of 15.0 μm or less, in which an oxygen atom concentration on a surface of the boron nitride particles C detected by X-ray photoelectron spectroscopic analysis is 1.5 atomic % or more. | 2022-07-07 |
20220213372 | (CO)POLYMER MATRIX COMPOSITES COMPRISING THERMALLY-CONDUCTIVE PARTICLES AND ENDOTHERMIC PARTICLES AND METHODS OF MAKING THE SAME - (Co)polymer matrix composites including a porous (co)polymeric network; a multiplicity of thermally-conductive particles, and a multiplicity of endothermic particles distributed within the (co)polymeric network structure; wherein the thermally-conductive particles and endothermic particles are present in a range from 15 to 99 weight percent, based on the total weight of the particles and the (co)polymer (excluding the solvent). Optionally, the (co)polymer matrix composite volumetrically expands by at least 10% of its initial volume when exposed to a temperature of at least 135° C. Methods of making and using the (co)polymer matrix composites are also disclosed. The (co)polymer matrix composites are useful, for example, as heat dissipating or heat absorbing articles, as fillers, thermal interface materials, and thermal management materials, for example, in electronic devices, more particularly mobile handheld electronic devices, power supplies, and batteries. | 2022-07-07 |
20220213373 | HEAT-REMOVING SHEET - A heat-removing sheet includes a plurality of endothermic particles and a chemically cured or radiation cured resin binding the endothermic particles together. The heat-removing sheet includes the endothermic particles at greater than 60 weight percent, has a flexural modulus of less than 3000 MPa and a flexural strength of greater than 0.15 MPa. The heat-removing sheet is a single free-standing layer. | 2022-07-07 |
20220213374 | HIGH DENSITY BRINE WITH LOW CRYSTALLIZATION TEMPERATURE - A wellbore fluid comprising a first aqueous base fluid and a plurality of silica nanoparticles suspended in the first aqueous base fluid. The nanoparticles are present in the fluid in an amount to have an effect of decreasing a crystallization temperature by at least 4 to 55° F. as compared to a second aqueous base fluid without the silica nanoparticles. | 2022-07-07 |
20220213375 | SPENT JET-ENGINE OIL AS DRILLING LUBRICANT - A water-based drilling fluid having spent jet-engine oil as a lubricant. A system and method for utilizing the spent jet-engine oil and the water-based drilling fluid to drill a borehole in a subterranean formation in the Earth crust. | 2022-07-07 |
20220213376 | OXIDIZING GASSES FOR CARBON DIOXIDE-BASED FRACTURING FLUIDS - Unconventional hydrocarbon source rock reservoirs can contain the organic material kerogen, intertwined with the rock matrix. The kerogen can alter the tensile strength of the rock and contribute to higher fracture energy needs. To degrade kerogen and other organic materials, oxidizing gasses are dissolved in carbon dioxide (CO | 2022-07-07 |
20220213377 | Scale Deposition Inhibiting Scavenger Compositions And Their Use - A method of treating a fluid to reduce the level of H | 2022-07-07 |
20220213378 | COMPOSITION AND METHOD FOR HYDRAULIC FRACTURING AND EVALUATION AND DIAGNOSTICS OF HYDRAULIC FRACTURES USING INFUSED POROUS CERAMIC PROPPANT - A composition and method for hydraulically fracturing an oil or gas well to improve the production rates and ultimate recovery using a porous ceramic proppant infused with a chemical treatment agent is provided. The chemical treatment agent may be a tracer material that provides diagnostic information about the production performance of a hydraulic fracture stimulation by the use of distinguishable both water soluble and hydrocarbon soluble tracers. The tracer can be a biological marker, such as DNA. The porous ceramic proppant can be coated with a polymer which provides for controlled release of the chemical treatment agent into a fracture or well bore area over a period of time. | 2022-07-07 |
20220213379 | QUANTUM DOT-CONTAINING MATERIAL, METHOD OF PREPARING QUANTUM DOT-CONTAINING MATERIAL, CROSS-LINKED MATERIAL OF QUANTUM DOT-CONTAINING MATERIAL, METHOD OF PREPARING CROSS-LINKED MATERIAL, AND LIGHT-EMITTING DEVICE INCLUDING CROSS-LINKED MATERIAL - Disclosed are a quantum dot-containing material, a method of preparing the quantum dot-containing material, a cross-linked material of the quantum dot-containing material, and a method of preparing the same, and a light-emitting device comprising the quantum dot-containing material. The quantum dot-containing material may include a quantum dot and an organic group chemically bound to a surface of the quantum dot, the organic group may include an azide group and a charge transport group, and the charge transport group may not be an unsubstituted benzene group. | 2022-07-07 |
20220213380 | QUANTUM DOT, CURABLE COMPOSITION COMPRISING THE SAME, CURED LAYER USING THE COMPOSITION, AND COLOR FILTER INCLUDING THE CURED LAYER - Disclosed are a surface-modified quantum dot, a curable composition including the quantum dot, a cured layer, and a color filter. | 2022-07-07 |
20220213381 | NANOCRYSTAL EMISSIVE MATERIALS, LIGHT EMITTING ELEMENT, AND PROJECTOR LIGHT SOURCE BASED ON THESE MATERIALS - Active materials for light emitting elements useful for light source apparatus and projector devices are provided. In particular, a light emitting element includes emissive semiconductor nano(crystal)material(s) (NC). Further, a light source apparatus, includes at least one light emitting element according to the present disclosure. The present disclosure also relates to a projector device, comprising a light source apparatus, comprising at least one light emitting element according to the present disclosure. Moreover, the present disclosure relates to methods of obtaining embedded semiconductor nano(crystal)material(s) and NC films. | 2022-07-07 |
20220213382 | SUBSTRATE PROCESSING DEVICE AND ETCHING LIQUID - A substrate processing method includes holding a substrate; and supplying an etching liquid to the substrate held in the holding of the substrate. The etching liquid contains an etching agent configured to etch a metal-based first material and a silicon-based second material exposed on the substrate and a protection agent configured to react with the second material between the first material and the second material to form a protection layer on a surface of the second material. Here, the etching agent is a liquid which contains fluorine atoms and an organic solvent and substantially does not contain water, and the protection layer protects the second material from etching with the etching agent. | 2022-07-07 |
20220213383 | RUST INHIBITOR, RUST INHIBITOR COMPOSITION, COATING FORMATION MATERIAL, COATING, AND METAL COMPONENT - The present invention provides: a rust inhibitor that has excellent rust inhibiting and anti-corrosive properties, not only on iron members, but also on non-iron metal members, and can prevent rust, and corrosion over long periods; a rust, inhibitor composition that, contains the rust inhibitor, a coating formation material; a coating obtained from the rust inhibitor, the rust inhibitor composition, or the coating formation material; and a metal component that comprises the coating. This rust inhibitor contains at least one compound represented by a Chemical Formula (1). | 2022-07-07 |
20220213384 | POLYMERIZABLE LIQUID CRYSTAL COMPOSITION, CURED PRODUCT, OPTICAL FILM, POLARIZING PLATE, AND IMAGE DISPLAY DEVICE - Provided is a polymerizable liquid crystal composition with which an image display device having an excellent contrast can be manufactured; and a cured product, an optical film, a polarizing plate, and an image display device. The polymerizable liquid crystal composition contains a compound represented by Formula (I). L | 2022-07-07 |
20220213385 | COMPOSITION FOR FORMING POLARIZER, POLARIZER, LAMINATE, AND IMAGE DISPLAY DEVICE - Provided is a composition for forming a polarizer from which a polarizer with an excellent surface state can be formed, a polarizer, a laminate, and an image display device. The composition for forming a polarizer includes a polymerizable liquid crystal compound which has two or more rings selected from the group consisting of an aromatic ring and an alicyclic ring and two polymerizable groups and exhibits a liquid crystal state of a smectic phase, a monofunctional polymerizable compound which has two or more rings selected from the group consisting of an aromatic ring and an alicyclic ring and one polymerizable group, and a dichroic material, in which the number of atoms NA of the polymerizable liquid crystal compound and the number of atoms NB of the monofunctional polymerizable compound satisfy Expression (1), 0.35≤NB/NA≤0.65 (1). | 2022-07-07 |
20220213386 | AN INTEGRATED METHOD OF PYROLYSIS CARBONIZATION AND CATALYSIS FOR BIOMASS AND A DEVICE THEREOF - The invention provides a method of pyrolysis carbonization and catalysis for biomass, which comprises: using waste biomass from agriculture and forestry as raw materials, conducting pyrolysis carbonization reaction at 630˜720° C. under oxygen-limited or oxygen-insulation conditions, obtaining biochar and bio-tar-containing pyrolysis oil-gas mixture after gas-solid separation of the products; treating the bio-tar-containing pyrolysis oil-gas mixture obtained with a biochar catalyst at 690˜850° C., carrying out bio-tar catalytic cracking to obtain small molecular combustible gas and light bio-tar, preserving heat and ageing the biochar obtained at 530˜650° C. then making a kind of biochar catalyst. The invention further provides an integrated device used for the method of pyrolysis carbonization and catalysis for biomass, comprising: a spiral feeder, a pyrolysis carbonization device and a catalysis device. The method of pyrolysis carbonization and catalysis for biomass and the device thereof according to the invention can solve the problems presented in the existing methods such as high energy consumption, high cost, and low utilization ratio of energy. | 2022-07-07 |
20220213387 | Synthetic Source Rock with Tea - A synthetic source rock including roasted tea powder and inorganic material. A technique for preparing the synthetic source rock, including grinding tea leaves to give tea powder, roasting the tea powder at a roasting temperature to give a roasted tea powder, and determining composition and porosity of the roasted tea powder. | 2022-07-07 |
20220213388 | HYDROTREATMENT OF OXYGENATE FEEDSTOCK WITH LIQUID RECYCLE FROM LOW PRESSURE SEPARATOR - A hydrotreatment unit for an oxygenate feedstock is provided, the unit including: a hydrotreatment reactor; a first cooling unit; a high-pressure separator and a low pressure flash unit. The hydrotreatment unit is arranged to feed at least a part of the hydrogen-rich stream from the high-pressure separator to the hydrotreatment reactor; and the hydrotreatment unit is arranged to feed a part of the degassed hydrocarbon-rich stream from said low pressure flash unit as a hydrocarbon recycle stream to the hydrotreatment reactor. Also, a method for hydrotreating an oxygenate feedstock using the hydrotreatment unit. | 2022-07-07 |
20220213389 | FLUID CATALYTIC CRACKING PROCESS OF CRUDE LIGNIN OIL (CLO) - A FCC process including the steps of
| 2022-07-07 |
20220213390 | PROCESSES FOR UTILISATION OF PURIFIED COAL TO UPGRADE REFINERY PROCESS COMPONENTS IN THE MANUFACTURE OF PETROLEUM COKE - Processes for the production of coke, and one or more volatile products comprise the steps of: (i) providing a purified coal product (PCP), wherein the PCP is in particulate form, and wherein at least about 90% v of the particles are no greater than about 100 μm in diameter; wherein the PCP has an ash content of less than about 10% m and a water content of less than around 5% m; (ii) combining the PCP with a liquid residue oil to create a combined solid-liquid blend, wherein the solid-liquid blend comprises at least around 0.1% m and at most around 30% m PCP; (iii) subjecting the solid-liquid blend to a temperature in excess of 375° C. for a time period sufficient to induce cracking of at least 1% of the PCP particles to generate the one or more volatile products, and (iv) producing coke from the product of step (iii). | 2022-07-07 |
20220213391 | ADSORPTION SYSTEMS AND PROCESSES FOR RECOVERING PNA AND HPNA COMPOUNDS FROM PETROLEUM BASED MATERIALS AND REGENERATING ADSORBENTS - A process for treating a hydrocarbon stream to remove polynuclear aromatic (PNA) and heavy polynuclear aromatic (HPNA) compounds includes contacting the hydrocarbon stream with an adsorbent in an adsorption unit to adsorb the PNA and HPNA compounds onto the adsorbent to produce a treated hydrocarbon stream and regenerating the adsorbent. Regenerating the adsorbent may include contacting the adsorbent with a solvent comprising a disulfide oil, such as a disulfide oil effluent from a mercaptan oxidation unit. The solvent comprising the disulfide oil desorbs the PNA and HPNA compounds from the adsorbent into the solvent to produce a desorption effluent. The treated hydrocarbon stream can be passed to a hydrocracking unit that hydrocracks the treated hydrocarbon stream to produce a hydrocracker effluent that includes greater value petrochemical products or intermediates. | 2022-07-07 |
20220213392 | SYSTEMS AND PROCESSES FOR TREATING DISULFIDE OIL - A process for treating a disulfide oil composition that includes combining a supercritical water stream, a hydrogen stream, and a disulfide oil composition in a mixing device to create a combined disulfide feed stream; introducing the combined disulfide feed stream into a supercritical water hydrogenation reactor operating at a temperature greater than a critical temperature of water and a pressure greater than a critical pressure of water; and at least partially converting the combined disulfide feed stream to an upgraded disulfide product. | 2022-07-07 |
20220213393 | SYSTEMS AND PROCESSES FOR HYDROCARBON UPGRADING - A process for upgrading a hydrocarbon-based composition that includes combining a supercritical water stream, a hydrogen stream, and a pressurized, heated hydrocarbon-based composition in a mixing device to create a combined feed stream. The process further includes introducing the combined feed stream into a supercritical water hydrogenation reactor operating at a temperature greater than a critical temperature of water and a pressure greater than a critical pressure of water, and at least partially converting the combined feed stream to an upgraded product. | 2022-07-07 |
20220213394 | PROCESSES FOR CATALYZED RING-OPENING OF CYCLOPARAFFINS - Embodiments of the disclosure include processes for selective ring-opening of cycloparaffins in hydrocarbon feeds to produce hydrocracked cycloparaffins. In particular, the process comprises contacting a hydrocarbon feed comprising cycloparaffins with hydrogen and a catalyst comprising an unsulfided, low-acidity, metal-containing zeolite under hydrocracking conditions; wherein the metal is selected from the group consisting of platinum, nickel, rhodium and mixtures thereof. The processes are useful for upgrading petroleum streams to lubricant base stocks. | 2022-07-07 |
20220213395 | INTEGRATED FCC AND AROMATIC RECOVERY COMPLEX TO BOOST BTX AND LIGHT OLEFIN PRODUCTION - In accordance with one or more embodiments of the present disclosure, a process for converting diesel to products comprising light olefins, benzene-toluene-xylenes (BTX), fluid catalytically cracked naphtha, pyrolysis gasoline, and pyrolysis fuel oil includes: introducing a diesel feedstream to a diesel hydrodesulfurization unit to produce a desulfurized diesel stream; introducing the desulfurized diesel stream to a fluid catalytic cracking (FCC) unit to produce the fluid catalytically cracked naphtha, a light gas stream, and a cycle oils stream; introducing the fluid catalytically cracked naphtha to an aromatic recovery complex to produce the BTX and an aromatic bottoms stream; and introducing a paraffinic fraction of the light gas stream to a steam cracking unit to produce a light olefins stream, the pyrolysis gasoline, and the pyrolysis fuel oil. | 2022-07-07 |
20220213396 | DISTILLATE HYDROCRACKING PROCESS WITH AN N-PARAFFINS SEPARATION STEP TO PRODUCE A HIGH OCTANE NUMBER ISOMERATE STREAM AND A STEAM PYROLYSIS FEEDSTOCK - In accordance with one or more embodiments of the present disclosure, process for reforming a diesel feedstock to convert diesel to a gasoline blending component includes hydrodesulfurizing and hydrodenitrogenizing the diesel feedstock to reduce a sulfur content of the diesel feedstock and a nitrogen content of the diesel feedstock; hydrocracking the hydrodesulfurized and hydrodenitrogenized diesel feedstock over a metal-containing diesel hydrocracking catalyst comprising at least one zeolite to produce an isomerate fraction; and separating the isomerate fraction into a first stream enriched in n-paraffins and a second stream enriched in iso-paraffins and naphthenes. | 2022-07-07 |
20220213397 | GASIFICATION OF PLASTICS AND SOLID FOSSIL FUELS TO PRODUCE ORGANIC COMPOUNDS - Pre-ground plastics of small particle size not more than 2 mm are co-fed into a solid fossil fuel fed entrained flow partial oxidation gasifier. High solids concentrations in the feedstock stream can be obtained without significant impact on the feedstock stream stability and pumpability. A consistent quality of syngas can be continuously produced, including generation of carbon dioxide and a carbon monoxide/hydrogen ratio while stably operating the gasifier and avoiding the high tar generation of fluidized bed or fixed bed waste gasifiers and without impacting the operations of the gasifier. The subsequent syngas produced from this material can be used to produce a wide range of chemicals. | 2022-07-07 |
20220213398 | PROCESS AND APPARATUS FOR PRODUCING SYNTHESIS GAS THROUGH THERMOCHEMICAL CONVERSION OF BIOMASS AND WASTE MATERIALS - The present invention provides a process and apparatus for converting feedstock comprising biomass and/or carbon-containing solid waste material to synthesis gas. The process comprises supplying the feedstock to a gasifier comprising a fluidized bed zone and a post-gasification zone and contacting the feedstock with a gasification agent at a plurality of different operating temperatures based on the ash softening temperature of the feedstock and finally recovering the synthesis gas. The apparatus is configured to perform the process and comprises a plurality of nozzles arranged at an acute angle relative to a horizontal plane of the gasifier. | 2022-07-07 |
20220213399 | CO2-PHILIC CROSSLINKED POLYETHYLENE GLYCOL-BASED MEMBRANES FOR ACID AND SOUR GAS SEPARATIONS - This disclosure relates to CO | 2022-07-07 |
20220213400 | Process to Produce Low Shear Strength Base Oils - A lubricating fluid comprising a base oil, which utilizes carboxylic esters of carboxyl di-end-capped-polyethylene glycols, or a mixture thereof with carboxylic esters of carboxyl di-end-capped-polytetramethylene glycols, to improve low temperature performance, as well as minimize elastohydrodynamic shear strength, and enables the production of high efficiency fluids with enhanced low temperature properties for machines or machine elements that operate in the elastohydrodynamic regime in lubrication. | 2022-07-07 |
20220213401 | LUBRICATING OIL COMPOSITIONS - The present disclosure generally relates to a lubricating oil composition comprising: a major amount of an oil of lubricating viscosity, one or more detergents comprising at least one alkylhydroxybenzoate compound, and an ashless sulfur compound. Also provided is a method of lubricating an engine with said lubricating oil composition. | 2022-07-07 |
20220213402 | METHOD FOR REDUCING AND/OR CONTROLLING ABNORMAL GAS COMBUSTION IN A MARINE ENGINE OR A CONTROLLED-IGNITION ENGINE - The present invention relates to the use of a copolymer (C) comprising repeat units corresponding to alkyl methacrylate monomers, said monomers comprising at least one or more monomer(s) (A), the same or different, selected from among (C6-C10) alkyl methacrylate monomers; one or more monomer(s) (B), the same or different, selected from among (C10-C18) alkyl methacrylate monomers, in a lubricant composition comprising at least one base oil for reducing and/or controlling abnormal gas combustion in an engine, preferably a marine engine or a controlled-ignition engine. | 2022-07-07 |
20220213403 | A PRESERVATIVE COMPOSITION - The present invention provides a preservative composition to prevent and/or reduce corrosion on a metal surface comprising an anhydride-derived amide and an oil and/or a solvent, wherein the anhydride-derived amide is present in an amount in the range of from 0.01-50 wt %, based on the total weight of the preservative composition. The invention also provides a preservative composition comprising in addition a sulfonate composition; a metal article having a coating thereon which coating comprises the present preservative composition; and the use of the present preservative composition to prevent and/or reduce corrosion on a metal surface. | 2022-07-07 |
20220213404 | LUBRICANT, ELECTRIC CONTACT, CONNECTOR TERMINAL, AND WIRE HARNESS - Provided are a lubricant whereby particles including a fluororesin can be dispersed and adhered to the surface of an object without the use of a fluorine-based oil as a base oil, an electrical contact having such a lubricant on the surface thereof, and a connector terminal and a wire harness provided with such an electrical contact. The present invention provides a lubricant containing a base oil and resin particles including a trifluoroethylene resin, the content of the resin particles being more than 10 mass % with respect to the mass of the base oil. The present invention also provides an electrical contact | 2022-07-07 |
20220213405 | LUBRICATION OF TRANSFER PLATE USING AN OIL OR OIL IN WATER EMULSIONS - This disclosure relates to transfer plate lubricant compositions and methods of transporting open containers across stationary transfer plates. | 2022-07-07 |
20220213406 | OIL EXTRACTION APPARATUS - An oil extraction apparatus includes an ultrasonication vessel that receives raw plant material and ethanol. An ultrasonication probe ultrasonicates the raw plant material received in the ultrasonication vessel and generates a mixture including ultrasonicated raw plant material, plant oil, and ethanol. A collection vessel is in fluid communication with the ultrasonication vessel. The collection vessel receives a mixture including plant oil and ethanol from the ultrasonication vessel. A heater heats the collection vessel to separate ethanol from the mixture including plant oil and ethanol. A reclamation vessel is in fluid communication with the collection vessel. The reclamation vessel receives separated ethanol from the mixture including plant oil and ethanol. An ethanol collection tube is connected with the reclamation vessel. The ethanol collection tube is arranged to carry separated ethanol from the mixture including plant oil and ethanol to the reclamation vessel from the collection vessel. | 2022-07-07 |
20220213407 | METHOD FOR LOWERING THE OXIDISING POWER OF A LIQUID OR SEMI-LIQUID ORGANIC COMPOSITION - A method for lowering the oxidising power of a liquid or semi-liquid organic composition, as well as of the so-called free organic composition that can be obtained by this method. Also, a free organic composition that can be obtained by the method of the invention, as well as a food, cosmetic or chemical composition or a pharmaceutical composition containing the composition treated by the method. | 2022-07-07 |
20220213408 | POLYMERIC CLEANING BOOSTER - A liquid laundry additive is provided, comprising a cleaning booster polymer having structural units of a monoethylenically unsaturated carboxylic acid monomer; structural units of an ethylenically unsaturated monomer of formula (I) | 2022-07-07 |
20220213409 | ANTIMICROBIAL MULTI-PURPOSE CLEANER AND METHODS OF MAKING AND USING THE SAME - The present disclosure relates to multi-purpose cleaning compositions, methods of manufacturing the multi-purpose cleaning compositions, and methods of using the multi-purpose cleaning compositions to clean a surface. Beneficially the multi-purpose cleaning compositions are capable of removing soil and providing antimicrobial activity. The compositions are especially useful on hard surfaces and are preferably low streaking. | 2022-07-07 |
20220213410 | COMPOUND AND DETERGENT COMPOSITION - A furan-based surfactant comprising a beta sulphonate head group, a furan and a C10-20 hydrophobic group which is either attached directly to the furan or by way of a linker. A laundry composition or a hand dish wash cleaning composition comprising said surfactant. | 2022-07-07 |
20220213411 | CLEANING COMPOSITIONS EMPLOYING EXTENDED CHAIN ANIONIC SURFACTANTS - The invention discloses synergistic combinations of surfactant blends and cleaning compositions employing the same. In certain embodiments a surfactant system is disclosed which includes an extended anionic surfactant with novel linker surfactants including one or more of an alkyl glycerol ether, an ethoxylated alkyl glycerol ether, an alcohol ethoxylate and/or a Gemini surfactant. This system forms emulsions with, and can remove greasy and oily stains, even those comprised of non-trans fats. The compositions may be used alone, as a pre-spotter or other pre-treatment or as a part of a soft surface or hard surface cleaning composition. | 2022-07-07 |
20220213412 | Ethoxylated Glycerol Ester-Containing Detergent For Machine Dishwashing - A detergent composition for machine dishwashing comprising Z1) one or more ethoxylated glycerol esters of formula (I), | 2022-07-07 |
20220213413 | PYRITHIONE PRESERVATIVE SYSTEM AND C1-C12 ETHOXYLATED ALCOHOL IN HARD SURFACE CLEANING PRODUCTS - Solid rinse aid compositions and methods of making and using the same are disclosed. Solid rinse aid compositions include in a single concentrate composition a pyrithione preservative system to replace conventional preservatives in the isothiazolinone family, such as chloromethylisothiazolinone. Beneficially, the pyrithione preservative systems eliminate the need for any personal protective equipment to handle the solid rinse aid compositions. Methods of making and use using the rinse aids are also disclosed. | 2022-07-07 |
20220213414 | A POLYMER FOR CLEANING BOOSTING - A liquid laundry additive is provided, comprising a cleaning booster polymer having structural units of a monoethylenically unsaturated carboxylic acid monomer; structural units of an ethylenically unsaturated monomer of formula (I) | 2022-07-07 |
20220213415 | SUBSTRATE PATTERN FILLING COMPOSITION AND USE OF THE SAME - To provide a substrate pattern filling composition capable of suppressing pattern collapse and a method for using the same. A substrate pattern filling composition comprising a first solute (A), a second a solute (B) and a solvent (C), and a method for using the same. | 2022-07-07 |
20220213416 | ACTIVE COMPOSITION DELIVERY SYSTEM - An active composition delivery system having a water-soluble top layer, a water-soluble bottom layer, and an active composition disposed therebetween. The water-soluble top and bottom layers are joined together to form a pouch sized and shaped to hold an amount of an active composition. The active composition delivery system further includes a plurality of water-soluble joints, joining the top and bottom water-soluble layers. | 2022-07-07 |
20220213417 | COMPOSITIONS AND METHODS FOR BREWING SOUR BEER - The present invention relates to the unexpected discovery of a new strain of yeast, dubbed GY7B, which is related to, but genetically and phenotypically distinct from, | 2022-07-07 |
20220213418 | METHOD FOR GROWING AQUATIC ORGANISMS CAPABLE OF PHOTOSYNTHESIS IN A CONTROLLED AQUEOUS ENVIRONMENT - The present invention relates to a method for growing aquatic organisms capable of photosynthesis in a controlled aqueous environment and a system for carrying out said method. The invention provides a method and system for growing aquatic organisms capable of photosynthesis under controlled conditions and which uses low energy input. | 2022-07-07 |
20220213419 | Plug Flow Fermenter for a Biogas Plant - The invention relates to a plug flow fermenter ( | 2022-07-07 |
20220213420 | BIOPROCESSING SYSTEM AND CONSUMABLE BAG FOR A BIOPROCESSING SYSTEM - A bioreactor system, includes a base platform, a lid received atop the base platform and defining an interior space for receiving a bioprocessing bag, and a tubing management system supporting a tubing array a distance above the base platform and providing a means for quickly connecting and/or disconnecting a fluid supply line. | 2022-07-07 |
20220213421 | DEVICE FOR GROWING MICROORGANISMS - A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. | 2022-07-07 |
20220213422 | METHODS AND SYSTEMS FOR INTRACELLULAR DELIVERY AND PRODUCTS THEREOF - The present disclosure provides methods and systems for cell processing, including delivery of substances into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more substances such as therapeutic or gene-editing reagents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems. | 2022-07-07 |
20220213423 | POLYLACTIDE CELL CULTURE CONTAINERS AND USE IN CELL CULTURE - 2D and 3D cell culture containers formed from blends of poly-L-lactide and poly-D-lactide provide growth surfaces for adherent cells and do not require surface treatment or coating to support mammalian cell growth. The cell culture containers are transparent, heat tolerant, and are environmentally degradable and suitable for composting in landfills. | 2022-07-07 |
20220213424 | CULTURE APPARATUS FOR DRUG DISCOVERY RESEARCH - A cell culture apparatus having a cell-holding container and a pinholder-shaped member comprising needle-shaped bodies arranged on a substrate, wherein a protruding part is formed in the center of the bottom surface of the cell-holding container, a recessed part is formed between the center and a side wall, and through-holes through which the needle-shaped bodies penetrate are established on the bottom surface of the recessed part; the needle-shaped bodies are arranged in correspondence with the positions of the through-holes; and the pinholder-shaped member is arranged, such that a tip-side portion of each of the needle-shaped bodies penetrates through the corresponding through-hole from the bottom surface side or upper surface side of the cell-holding container. | 2022-07-07 |
20220213425 | CELL SCAFFOLD COMPRISING AN ELECTRONIC CIRCUIT - The present invention generally relates to nanoscale wires and nanoelectronics, which in some aspects may be embedded in biological structures, such as tissues, organoids, organs, organisms, and the like. For example, one aspect is generally directed to stretchable cell scaffolds, which may be connected to electrical circuits. In some cases, a biological structure may form around the scaffold and deform the cell scaffold as it forms, thereby causing the cell scaffold to become embedded within the biological structure. The scaffold may be connectable in certain embodiments to an external device, e.g., to determine a property of the cell scaffold (e.g., an electrical property), and/or to apply a stimulus (e.g., an electrical stimulus) to the biological structure. Other aspects of the invention are generally directed to methods of making or using such cell scaffolds, kits including such cell scaffolds, biological structures containing such cell scaffolds, or the like. | 2022-07-07 |
20220213426 | CELL CULTURE APPARATUS AND MEDIUM EXCHANGE METHOD - By swinging a culture vessel before aspiration removal of a medium, a centrally dense state ( | 2022-07-07 |
20220213427 | BIOREACTOR DEVICE AND METHODS - An apparatus is provided for the production of biomass or a bioproduct, the apparatus comprising: at least one elongate bioreactor, the bioreactor comprised of at least one outer membrane layer that defines a substantially tubular compartment that is capable of being filled with a liquid or gel, wherein the membrane layer is comprised of a material that is permeable to gas transfer across the membrane layer. A chamber is provided comprising walls that define and enclose a gaseous atmosphere within. At least a part of the bioreactor is located inside the chamber. A control system controls the composition of the atmosphere within the chamber and gas transfer occurs across the membrane layer of the bioreactor between the tubular compartment and the atmosphere comprised within the chamber. Methods of using the apparatus in order to manufacture biomass are also provided. | 2022-07-07 |
20220213428 | APPARATUS, METHODS AND COMPOSITION FOR SYNTHESIS OF CANNABINOID COMPOUNDS - The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. | 2022-07-07 |
20220213429 | METHOD AND MEANS FOR OPTIMIZING BIOTECHNOLOGICAL PRODUCTION - A new method for the automatic generation and validation of a Digital Twin for the production of biotechnological products and the application of the Digital Twin for the purpose of increasing product concentration, productivity, biomass concentration and product quality by optimizing media composition and/or feeding profiles. The Digital Twin can be linked directly to production for online optimization or offline for decision support. | 2022-07-07 |
20220213430 | METHODS OF TISSUE PROCESSING - The present technology provides a method for the effective participation of tissue, for example, amniotic, chorionic, umbilical cord, or a combination thereof to create a flowable suspension for therapeutic purposes. In contrast to other technologies, the present technology does not require cryogenic milling or pre-freezing to harden the tissue for effective particulation and is further capable of preserving the viability of endogenous cell types in contrast to previous methodologies. | 2022-07-07 |
20220213431 | METHODS FOR OVERCOMING GLUTAMINE DEPRIVATION DURING MAMMALIAN CELL CULTURE - The present invention pertains to methods of producing polypeptide of interest in cell cultures lacking glutamine. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with nucleic acid synthesis precursors and/or corticosteroids. | 2022-07-07 |
20220213432 | Transplantable cell composition comprising eukaryotic cells in a nanofibrillar cellulose hydrogel, method for preparing thereof and use of nanofibrillar cellulose - The present application provides a method for preparing transplantable cell preparation, the method comprising culturing eukaryotic cells at conditions allowing the cells to coalesce and form cell aggregates, providing the cell aggregates in a nanofibrillar cellulose hydrogel to obtain a transplantable cell composition comprising eukaryotic cells in a nanofibrillar cellulose hydrogel matrix, and a transplantable cell preparation. The present application also provides the transplantable cell composition for use in a therapeutic method, and to use of nanofibrillar cellulose for preparing the transplantable cell composition. | 2022-07-07 |
20220213433 | Three-Dimensional Cross-Linked Scaffolds of Cord Blood Plasma and Their Use - The disclosure provides three-dimensional cross-linked scaffolds generated from cord blood plasma, and methods for making and using such scaffolds. | 2022-07-07 |
20220213434 | MODIFIED PLURIPOTENT CELLS - The invention discloses for the first time pluripotent cells, including induced pluripotent stem cells, embryonic stem cells, and hypo-immune pluripotent cells that are ABO blood type O Rhesus Factor negative and evade rejection resulting from blood type antigen mismatch. The invention further provides universally acceptable “off-the-shelf” pluripotent cells and derivatives thereof for generating or regenerating specific tissues and organs. | 2022-07-07 |
20220213435 | Stem Cell Aggregates and Methods for Making and Using - The invention is directed to compositions of cell aggregates and methods for making and using the cell aggregates where the aggregates comprise cells that are not embryonic stem cells but can differentiate into cell types of at least two of ectodermal, endodermal, and mesodermal embryonic germ layers, e.g., stem cells. | 2022-07-07 |
20220213436 | BRAIN ORGANOID MACHINE INTERFACE - The disclosure provides methods of making and systems comprising a brain organoid operably connected to a controlled device such that the brain organoid controls the controlled device. | 2022-07-07 |
20220213437 | PROTEIN-MODIFIED PLGA MICROSPHERE AND TISSUE-ENGINEERED NERVE CONSTRUCTED THEREWITH - A protein-modified PLGA microsphere can be used to construct tissue-engineered nerve. The microspheres are loaded with active substances for treating peripheral nerve injury and are bound to tissue-engineered nerves. It has been shown that the prepared tissue-engineered nerve effectively promotes nerve regeneration after peripheral nerve injury. | 2022-07-07 |
20220213438 | NUTRIENT COMPOSITIONS AND METHODS, KITS, AND CELL COMPOSITIONS FOR PRODUCING THE SAME - Compositions, kits, and methods for preparing nutrient compositions are provided. A nutrient composition can be a food composition such as a dairy composition. A nutrient composition may be produced by an engineered cell composition. Compositions, kits, and methods for preparing engineered cell compositions for producing nutrient compositions are also provided. An engineered cell composition may comprise genetically engineered cells, such as genetically engineered mammary or mammary-like cells. An engineered cell composition can be derived from mammalian stem cells, such as non-mammary adult stem cells. Related methods of characterization, such as for characterizing the engineered cell compositions or the nutrient compositions, are also provided. | 2022-07-07 |
20220213439 | SYSTEMS AND METHODS FOR MODULATING A CELL PHENOTYPE - The present disclosure relates to a method of modulating a phenotype a source population of cells to assume a desired phenotype. The method includes culturing the source cell population within an incubator configured to regulate the variable atmospheric parameters of oxygen level and total atmospheric pressure level. | 2022-07-07 |
20220213440 | DENDRITIC CELL IMMUNOTHERAPY - Methods of providing a targeted immune response in a subject comprising administration of a dendritic cell population are provided. In some aspects, dendritic cells are administered in conjunction with a Type I interferon (INF), a TLR-7 agonist, a TLR-9 agonist, AIMp1, a TLR-3 agonist, a retinoic acid inducible gene-1 (RIG-1)-like receptor ligand or a cytosolic DNA (CDS) receptor ligand and/or are administered to a tissue site proximal to diseased tissue. Therapeutic dendritic cell compositions are likewise provided. | 2022-07-07 |
20220213441 | EXTRACELLULAR MATRIX MATERIAL AND USES THEREOF - Provided are new methods for generating extracellular matrix material, compositions comprising the extracellular matrix material, and methods of using the extracellular matrix. | 2022-07-07 |
20220213442 | PREPARATION OF HUMAN ALLOGENEIC LIVER-DERIVED PROGENITOR CELLS - The invention relates to a process for the manufacture of a population of human allogeneic liver-derived progenitor cells (HALPC). The process comprises the use of a xeno- and serum-free culture medium comprising purified native or recombinant human serum albumin. | 2022-07-07 |
20220213443 | METHOD FOR COLD ATMOSPHERIC PLASMA EXOSOME THERAPY - A method for bioengineering exosomes for cancer treatment. The method comprises the steps of isolating target tumor cells from a tissue sample, culturing the isolated target tumor cells, treating the cultured target tumor cells with cold atmospheric plasma to release exosomes from the target tumor cells, and collecting the released exosomes. The step of culturing the target tumor cells may comprise seeding the isolated target tumor cells in wells and incubating the target tumor cells in a culture media, for example, for 24 hours. The culture media may comprise Dulbecco's Modified Eagle Medium, an antibiotic and 10% fetal bovine serum. The step of culturing the target tumor cells may further comprise replacing the culture media with Dulbecco's Modified Eagle Medium and an antibiotic. The target tumor cells may comprise, for example, one of U87 cells, KKU-055 cells and triple negative MDA-MB-231 cells. | 2022-07-07 |
20220213444 | COMPOSITIONS AND METHODS FOR CELLULAR REPROGRAMMING - Disclosed herein are compositions and methods for re-programming induced pluripotent stem cells, such as from a primed to naïve state. Further disclosed herein are methods of making said reprogrammed induced pluripotent stem cells by contacting the stem cells with another population of stem cells. | 2022-07-07 |
20220213445 | METHODS OF CULTURING HUMAN PLURIPOTENT CELLS - A culture of human pluripotent stem cells (hPSCs) is disclosed. In the culture, more than 50% of the hPSCs are formative hPSCs and are capable of renewing. Uses thereof are also disclosed. | 2022-07-07 |
20220213446 | METHOD FOR CULTURING CELLS INTO WHICH REPROGRAMMING FACTOR IS INTRODUCED - According to the present disclosure, there is provided a method for culturing cells into which a reprogramming factor is introduced including culturing cells into which a reprogramming factor is introduced; and recovering all cells into which the reprogramming factor is introduced and seeding and passaging at least part of the recovered cells in a medium. In addition, there is provided a method for culturing cells into which a reprogramming factor is introduced, including culturing cells into which a reprogramming factor is introduced; and inducing somatic cells different from pluripotent stem cells without passaging the cells into which the reprogramming factor is introduced. | 2022-07-07 |
20220213447 | AAV9 Affinity Agents - Provided herein are affinity agents comprising ligands that specifically bind adeno-associated virus. The affinity agents are useful for binding, isolation, and/or purification of adeno-associated virus. Further disclosed are amino acid sequences of binding motifs or polypeptides comprised by the ligands, and associated modifications of the binding motifs and/or polypeptides, as well as a method of making the affinity agents. | 2022-07-07 |
20220213448 | VIRAL VACCINES AND METHODS OF FORMING THE SAME - Provided herein is a method for producing an inactivated virus including a) heating the virus to a temperature sufficient to disrupt the virus membrane; b) exposing the virus of step (a) to a nucleic acid degrading enzyme; and c) cooling the virus to a temperature sufficient to reestablish the integrity of the virus membrane. Also provided herein is a vaccine produced using the instant method. | 2022-07-07 |
20220213449 | DHFR TUNABLE PROTEIN REGULATION - The present invention is related to compositions and methods for the regulated and controlled expression of proteins. | 2022-07-07 |
20220213450 | OPTIMIZED SUMF1 GENES AND EXPRESSION CASSETTES AND THEIR USE - This invention relates to polynucleotides comprising optimized SUMF1 open reading frame (ORF) sequences, vectors comprising the same, and methods of using the same for deliver) of the ORF to a cell or a subject and to treat disorders associated with aberrant expression of a SUMF1 gene or aberrant activity of a SUMF1 gene product in the subject, such as SUMF1 disease. | 2022-07-07 |
20220213451 | O-Mannosyltransferase Deficient Filamentous Fungal Cells and Methods of Use Thereof - The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such as | 2022-07-07 |
20220213452 | CONDITIONALLY ACTIVE HETERODIMERIC POLYPEPTIDES AND METHODS OF USE THEREOF - The present disclosure provides conditionally active, heterodimeric polypeptides. The conditionally active, heterodimeric polypeptides are active in the presence of a dimerizing agent that induces dimerization of the polypeptides of the heterodimer. A conditionally active, heterodimeric polypeptide of the present disclosure is useful in a variety of research and treatment methods, which are also provided. | 2022-07-07 |
20220213453 | DETERGENT COMPOSITIONS - The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. | 2022-07-07 |
20220213454 | NUCLEASE-MEDIATED GENOME EDITING - The invention related to the field of genetic engineering tools, methods and techniques for gene or genome editing. Specifically, the invention concerns isolated polypeptides having nuclease activity, host cells and expression vectors comprising nucleic acids encoding said polypeptides as well as methods of cleaving and editing target nucleic acids in a sequence-specific matter. The poly peptides, nucleic acids, expression vectors, host cells and methods of the present invention have application in many fields of biotechnology, including, for example, synthetic biology and gene therapy. | 2022-07-07 |
20220213455 | THERMOSTABLE CAS9 NUCLEASES - Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms. | 2022-07-07 |
20220213456 | NOVEL OMNI CRISPR NUCLEASES - The present invention provides a non-naturally occurring composition comprising a CRISPR nuclease comprising a sequence having at least 95% identity to the amino acid sequence selected from the group consisting of SEQ ID NOs: 1-4 or 149-166 or a nucleic acid molecule comprising a sequence encoding the CRISPR nuclease. | 2022-07-07 |
20220213457 | NUCLEIC ACID-GUIDED NICKASES - The present disclosure provides engineered nucleic acid-guided nickases and optimized scaffolds for making rational, direct edits to nucleic acids in live cells. | 2022-07-07 |
20220213458 | MAD NUCLEASES - The present disclosure provides new RNA-guided nuclease systems and engineered nickases for making rational, direct edits to nucleic acids in live cells. | 2022-07-07 |
20220213459 | VARIANT CAS12 PROTEINS WITH IMPROVED DNA CLEAVAGE SELECTIVITY AND METHODS OF USE - Bridge helix-modified variant Cas12a and Cas12b proteins having improved DNA cleavage selectivity in comparison to wild type versions of the Cas12a and Cas12b proteins, nucleic acids encoding the variant proteins, host cells containing the nucleic acids, and methods of their use. | 2022-07-07 |
20220213460 | UNGLYCOSYLATED LYSOSTAPHIN VARIANT PROTEIN - Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a | 2022-07-07 |
20220213461 | ASX-SPECIFIC PROTEIN LIGASES AND USES THEREOF - The present invention lies in the technical field of enzyme technology and specifically relates to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. The enzymes having Asx-specific ligase and cyclase are isolated from plants of the | 2022-07-07 |
20220213462 | RED BLOOD CELLS EXPRESSING VON WILLEBRAND FACTOR PROTEASE AND METHODS OF USE THEREOF - This disclosure provides methods and compositions for treating TTP based on transfusion of a relatively small number of genetically modified red blood cells. The genetically modified red blood cells express a fusion protein including a fragment of ADAMTS13 that is enzymatically active against von Willebrand factor (VWF). The fragments of ADAMTS13 can be resistant to the inhibitors, e.g., the auto-immune antibodies, which are responsible for the acquired form of TTP. | 2022-07-07 |
20220213463 | FE-S FUSION PROTEIN ACTING AS ELECTRON TRANSFER CHAIN, CARBON MONOXIDE FORMATE REDOX ENZYME MEDIATED THROUGH FES FUSION PROTEIN, STRAIN BCF12 DERIVED FROM THERMOCOCCUS WHEREIN ENZYME IS TRANSFORMED, AND USE THEREOF - The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel | 2022-07-07 |
20220213464 | HIERARCHICAL MAGNETIC NANOPARTICLE-ENZYME MESOPOROUS ASSEMBLIES EMBEDDED IN MACROPOROUS SCAFFOLDS - A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion. | 2022-07-07 |
20220213465 | SINGLE-STEP DNA PREPARATION FOR POLYMERASE CHAIN REACTION USING MAGNETIC CHITOSAN MICROPARTICLES - The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved. | 2022-07-07 |
20220213466 | MULTIPLEXED TESTING OF LYMPHOCYTES FOR ANTIGEN SPECIFICITY - Described herein is a method for determining a lymphocyte cell receptor chain sequence specific to a unique antigen, comprising: sorting a plurality of antigens into a plurality of reaction mixtures, wherein the sorting comprises adding a unique antigen of the plurality of antigens to a unique subset of the plurality of reaction mixtures such that two different unique antigens are not added to the unique subset; contacting each reaction with a biological sample comprising a plurality of lymphocytes; separating a target lymphocyte from a subset of the plurality of lymphocytes, wherein the target lymphocyte recognizes the unique antigen; after separating the target lymphocyte, sequencing nucleic acids of the target lymphocyte to obtain the lymphocyte receptor chain sequence, wherein the sequencing is performed by single-cell sequencing; and detecting the unique antigen, wherein the detecting comprises: computing a frequency of lymphocyte cells that express the lymphocyte receptor chain sequence. | 2022-07-07 |
20220213467 | ASSEMBLY OF PROTEIN COMPLEXES ON A CHIP - A method of assembling and immobilizing proteinaceous complexes is disclosed. In addition, methods of generating functional RNA polymerase and ribosomal subunits are disclosed. | 2022-07-07 |
20220213468 | METHODS FOR MAPPING PERSONALIZED TRANSLATOME - A low-input RNase footprinting approach is described for the rapid quantification of ribosome-protected fragments with 1,000-100,000 cells. The assay uses a simplified procedure for capturing ribosome-RNA complexes based on optimized RNase digestion. It simultaneously maps cytosolic and mitochondrial translation with single-nucleotide resolution. | 2022-07-07 |