28th week of 2014 patent applcation highlights part 40 |
Patent application number | Title | Published |
20140193827 | CHARACTERIZING A GLATIRAMER ACETATE RELATED DRUG PRODUCT - The present invention provides a process for characterizing a glatiramer acetate related drug substance or drug product comprising the steps of:
| 2014-07-10 |
20140193828 | MOLECULAR FLUX RATES THROUGH CRITICAL PATHWAYS MEASURED BY STABLE ISOTOPE LABELING IN VIVO, AS BIOMARKERS OF DRUG ACTION AND DISEASE ACTIVITY - The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides the necessary information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through one or more metabolic pathways of interest. By this method, a comparison between subjects and control subjects reveals the effects of the chemical entity or entities on the biomarkers. This, in turn, allows for the identification of potential therapeutic uses or toxicities of the compound. Combinations of compounds can also be systematically evaluated for complementary, synergistic, or antagonistic actions on the metabolic pathways of interest, using the methods of the present invention as a strategy for identifying and confirming novel therapeutic or toxic combinations of compounds. | 2014-07-10 |
20140193829 | MONOCLONAL ANTIBODIES AGAINST SCRAPIE PRION PROTEIN - The present invention relates to monoclonal antibodies against PrP | 2014-07-10 |
20140193830 | OPTICAL BIOSENSORS - Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity. | 2014-07-10 |
20140193831 | SEED TRAIT PREDICTION BY ACTIVITY-BASED PROTEIN PROFILING - The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and/or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality. In particular, the method comprises contacting the protein sample or the plant seed or plant seed lot with a chemical probe comprising a warhead being able to attach to an amino acid residue in or nearby an active site of the target protein under conditions allowing the formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe further comprises a reporter tag which is used to detect the conjugate, and wherein detection of the conjugate indicates the presence of the target protein in the protein sample and is used to predict or diagnose the plant seed trait. The present invention further relates to the use of such chemical probes for the prediction of plant seed traits. | 2014-07-10 |
20140193832 | DETECTION OF PROSTATE CANCER USING PSA GLYCOSYLATION PATTERNS - The present invention features novel methods for determining if a subject has prostate cancer. The present invention is based on the development of lectin immunosorbant assays which analyze α2,6-linked sialylation of total serum PSA by | 2014-07-10 |
20140193833 | ISOLATION AND DETECTION OF CANCER CELLS - Provided are methods of using polycarbonate filters to isolate and detect cancer cells in a biological fluid, particularly biological fluids, such as urine, that contain very low concentrations of cancer cells. The characterization of the isolated cells for the presence or absence of cancer specific proteins is useful for cancer diagnosis and prognosis. | 2014-07-10 |
20140193834 | BLOOD-BRAIN BARRIER PERMEABLE PEPTIDE COMPOSITIONS - Blood-brain barrier permeable peptide compositions that contain variable antigen binding domains from camelid and/or shark heavy-chain only single-domain antibodies are described. The variable antigen binding domains of the peptide compositions bind to therapeutic and diagnostic biomarkers in the central nervous system, such as the amyloid-beta peptide biomarker for Alzheimer's disease. The peptide compositions contain constant domains from human IgG, camelid IgG, and/or shark IgNAR. The peptide compositions include heavy-chain only single-domain antibodies and compositions with one or more variable antigen binding domain, bound to one or more constant domains. | 2014-07-10 |
20140193835 | CELL-BASED DETECTION OF APF THROUGH ITS INTERACTION WITH CKAP4 FOR DIAGNOSIS OF INTERSTITIAL CYSTITIS - An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC. | 2014-07-10 |
20140193836 | NOVEL MARKERS FOR DOPAMINERGIC NEURON PROGENITOR CELLS - The present invention provides a method for selecting dopaminergic neuron progenitor cells, which comprises detecting any one or more of markers selected from the group consisting of CD15 (SSEA-1), CD24, CD46, CD47, CD49b, CD57, CD58, CD59, CD81, CD90, CD98, CD147, CD184, Disalogangliosid GD2, SSEA-4, CD49f, SERINC4, CCR9, PHEX, TMPRSS11E, HTR1E, SLC25A2, Ctxn3, Cc17, Chrnb4, Chrna3, Kcnv2, Grm2, Syt2, Lim2, Mboat1, St3ga16, Slc39a12, Tacr1, Lrtm1, Dscam and CD201. | 2014-07-10 |
20140193837 | LUMINESCENT NANOPARTICLE COMPOSITIONS - A composition is disclosed which is capable of being used for detection, comprising a noble metal nanoparticle. The inventive compositions exhibit little interaction with serum proteins while exhibiting pH-dependent adsorption onto live cell membranes. The nanoparticles of the claimed invention are capable of interacting with cell membranes, which in turn permits the advantages of nanoparticle bio-imaging to be extended to many pH dependent biological processes such as targeting acidic tumor microenvironment. | 2014-07-10 |
20140193838 | METHOD FOR MEASURING SIALIC ACID IN IMMUNOGLOBULIN G AND IMMUNOGLOBULIN G ANTI-DOUBLE-STRANDED DNA ANTIBODIES - A method for measuring the amount of sialic acid in immunoglobulin G and immunoglobulin G anti-ds DNA antibodies is disclosed. The method for measuring the amount of sialic acid in immunoglobulin G in the present invention uses culture fluid, blood, plasma, or serum to directly measure the amount of sialic acid in immunoglobulin G. Also, using a mouse monoclonal antibody immunoglobulin G as a standard, which is diluted from 1000 ng/ml to 15.625 ng/ml in phosphate buffered saline (PBS), produces good results. The method for measuring the amount of sialic acid in immunoglobulin G anti-ds DNA antibodies has never been done and the present invention produces good results as well. | 2014-07-10 |
20140193839 | Smartphone Biosensor - A mobile computing device that includes an image sensor may be used to detect the result of a biomolecular assay. The biomolecular assay may be performed in an optical assay medium that provides an optical output in response to light from a light source, with the optical output indicating result. A wavelength-dispersive element may be used to disperse the optical output into spatially-separated wavelength components. The mobile computing device may be positioned relative to the wavelength-dispersive element such that different wavelength components are received at different locations on the image sensor. With the mobile computing device positioned in this way, the image sensor may be used to obtain one or more images that include the separated wavelength components of the optical output. A wavelength spectrum of the optical output may be determined from the one or more images, and the result may be determined from the wavelength spectrum. | 2014-07-10 |
20140193840 | CHROMATOGRAPHY FILTER PAPER-BASED ELISA - A chromatography filter paper-based ELISA includes following steps: providing a chromatography filter paper plate provided with a plurality of independent hydrophilic regions defined by at least one hydrophobic region; immobilizing an antigen of a sample onto one of the hydrophilic regions of the chromatography filter paper plate; and detecting the antigen with an ELISA process, wherein an antibody used in the ELISA process is a monoclonal antibody. The present invention has advantages in lower amount of loaded sample, higher sensitivity and rapid analysis in comparison to conventional ELISA. | 2014-07-10 |
20140193841 | FLUOROGENIC SEMICONDUCTOR NANOCRYSTALS - Fluorogenic semiconductor nanocrystals and compositions thereof are provided herein, including kits, assay systems and methods for their preparation and use. | 2014-07-10 |
20140193842 | NON-SPECIFIC REACTION INHIBITOR, METHOD FOR INHIBITING NON-SPECIFIC REACTION, AND KIT - Provided is a non-specific reaction inhibitor for achieving the accurate detection and quantitation of a trace component (a target substance) contained in a sample, in an immunoassay, by simply and effectively inhibiting a non-specific reaction associated with the measurement. | 2014-07-10 |
20140193843 | USE OF BASIC PROLIN-RICH LACRIMAL GENE PRODUCTS, SUCH AS OPIORPHIN, AS A BIOMARKER - The present invention relates to the use of Basic Prolin-rich Lacrimal protein (BPLP) gene products, such as Opiorphin, for establishing a prognosis, a diagnosis or the monitoring of a pathological state or of treatment efficacy in a subject and the related method of use. | 2014-07-10 |
20140193844 | Biomarkers for Ankylosing Spondylitis - There is described herein methods and peptides for detecting autoantibodies to NOG and/or SOST in a patient sample in order to diagnose of prognosticate Ankylosing Spondylitis in the patient. | 2014-07-10 |
20140193845 | USE OF SFRP-3 IN THE ASSESSMENT OF HEART FAILURE - Disclosed is a method for assessing heart failure in vitro including the steps of measuring in a sample the concentration of the marker SFRP-3, of optionally measuring in the sample the concentration of one or more other marker(s) of heart failure, and of assessing heart failure by comparing the concentration determined in for SFRP-3 and the concentration(s) determined for the optionally one or more other marker to the concentration of this marker or these markers as established in a reference population. Also disclosed are the use of SFRP-3 as a marker protein in the assessment of heart failure, a marker combination comprising SFRP-3 and a kit for measuring SFRP-3. | 2014-07-10 |
20140193846 | NOVEL APOC-I ISOFORMS AND THEIR USE AS BIOMARKERS AND RISK FACTORS OF ATHEROSCLEROTIC DISEASE - Novel isoforms of apolipoprotein C-I (apoC-I), namely apolipoprotein C-I | 2014-07-10 |
20140193847 | GLUTATHIONE ALKYLESTER ISOTOPOLOGUE AND METHOD FOR DETECTING REACTIVE METABOLITE - [Problem] To provide a novel isotope-labeled compound that can be used as a trapping agent and that is useful for picking out drug-candidate compounds that produce reactive metabolites. | 2014-07-10 |
20140193848 | SAMPLE VIAL CAP AND SAMPLE VIAL FOR USE IN PREPARING CYTOLOGICAL SPECIMEN AND METHOD OF PREPARING CYTOLOGICAL SPECIMEN - A cap configured for use with a specimen container includes a cap body having a top surface defining an opening therethrough, a torque pattern disposed on the top surface of the cap body, and a pierceable and self-resealing membrane disposed across the opening, where, when the membrane is intact or resealed, the membrane forms a fluid-tight seal across the opening. | 2014-07-10 |
20140193849 | GLUCOSE-PEG CONJUGATES FOR REDUCING GLUCOSE TRANSPORT INTO A CELL - There is provided a glucose-PEG conjugate comprising a PEG moiety conjugated to a linear glucose moiety at the C1 position of the glucose moiety. The glucose-PEG conjugate may be used to reduce glucose transport into a cell and may be used to treat a proliferative disorder. | 2014-07-10 |
20140193850 | HOLOGRAPHIC METHOD AND DEVICE FOR CYTOLOGICAL DIAGNOSTICS - The present invention discloses a method for analyzing a liquid cell sample, comprising the steps of: a) providing at least one liquid cell sample in a sample vial; b) obtaining data linked to the cells in the sample by performing differential digital holographic microscopy on said liquid cell sample in said sample vial. In a second aspect, the invention also provides for a system for analyzing a liquid cell sample, comprising (i) a differential digital holographic microscope comprising illumination means, a differential interferometer and a digital recording device connected to a processing device such as a computer; (ii) at least one exchangeable sample vial comprising a liquid cell (iii) a movable sample vial holder; characterized in that (iv) said sample vial holder is adapted to receive said sample vial; (v) said sample vial holder is adapted to position said sample vial such that the focal plane of the objective lens of said differential digital holographic microscope lies in the vial. | 2014-07-10 |
20140193851 | Detecting Individual Analytes by Means of Magnetic Flow Measurement - In a magnetic flow measurement, such as flow cytometry, individual analytes are detected in the through-flow. The analytes (e.g., cells) are marked with magnetic labels directly in the medium surrounding the analytes. The analytes are transported through the flow channel of a measuring device including at least one magnetic sensor. Using the magnetic marking of the analytes, the magnetic analyte diameter (r | 2014-07-10 |
20140193852 | ADIPOSE TISSUE COLLECTION AND PRE-PROCESSING DEVICES FOR USE IN LIPOSUCTION PROCEDURE - Devices and methods are described for pre-processing tissue samples containing cells of interest for various therapeutic procedures. Fibrous material may be extracted from the tissue sample through a shredding and separating device, resulting in a non-fibrous sample containing the cells of interest, which may then be washed and centrifuged to remove fluids, oil, and contaminants. The sample may then be digested in a large-volume container, and a piston within the container may push the resulting liberated cell suspension through a cell concentrator such as a hollow-fiber separation module. | 2014-07-10 |
20140193853 | BIOPROCESSING - Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor. | 2014-07-10 |
20140193854 | Method for Mass-Producing Antifreeze Protein Derived from Polar Yeast - The present invention relates to a method for mass-producing an antifreeze protein derived from a polar yeast, and more particularly, to a method for mass-producing an antifreeze protein derived from | 2014-07-10 |
20140193855 | METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF CHEMICALLY SYNTHESIZED NUCLEIC ACIDS - This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies. | 2014-07-10 |
20140193856 | CONTROL OF GENE EXPRESSION - The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided. | 2014-07-10 |
20140193857 | CENTRIFUGE TUBE DROPLET GENERATOR - A centrifuge tube droplet generator includes a centrifugally generated droplet system which accommodates a sample volume of below 20 microliters and below. The system has virtually no dead volume, is simple and inexpensive and can therefore be realized in a disposable unit. The system can be tailored to fit the format of standard desktop centrifuges which are ubiquitous in biological laboratories. | 2014-07-10 |
20140193858 | PRODUCTION OF VIRAL VECTORS - The present invention relates to methods and compositions for the production of viral vectors. In particular, the present invention provides methods and compositions for faster, higher titer and higher purity production of viral vectors (e.g. adenoviral vectors). In some embodiments, the present invention provides gutted and helper viruses with identical or similar termini. In other embodiments, the present invention provides terminal protein linked adenoviral DNA. In certain embodiments, the present invention provides template extended adenoviral DNA. | 2014-07-10 |
20140193859 | Temperature-dependent insertion of genetic material into genomic DNA - The present invention provides improved methods and reagents for insertion of genetic material into genomic DNA. | 2014-07-10 |
20140193860 | Low Sequence Bias Single-Stranded DNA Ligation - The invention provides compositions and methods for ligating single stranded nucleic acids wherein the ligation is based on fast, efficient, and low-sequence bias hybridization of an acceptor molecule with a donor molecule. In one embodiment, the structure of the donor molecule comprises a stem-loop intramolecular nucleotide base pairing (i.e., hairpin) and a 3′-overhang region such that the overhang is able to hybridize to nucleotides present in the 3′ end of the acceptor molecule. | 2014-07-10 |
20140193861 | Methods Of Producing 7-Carbon Chemicals Via Aromatic Compounds - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes. | 2014-07-10 |
20140193862 | Methods Of Producing 7-Carbon Chemicals Via Carbon Chain Elongation Associated With Cyclohexane Carboxylate Synthesis - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from | 2014-07-10 |
20140193863 | Methods Of Producing 7-Carbon Chemicals Via C1 Carbon Chain Elongation Associated With Coenzyme B Synthesis - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis. | 2014-07-10 |
20140193864 | Methods Of Producing 7-Carbon Chemicals Via Pyruvate And Succinate Semialdehyde Aldol Condensation - This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate. | 2014-07-10 |
20140193865 | Methods Of Producing 7-Carbon Chemicals From Long Chain Fatty Acids Via Oxidative Cleavage - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as | 2014-07-10 |
20140193866 | Compositions and Methods for Making and Modifying Oils - The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof. | 2014-07-10 |
20140193867 | MICROBIAL ODD CHAIN FATTY ACIDS - Engineered bacteria that produce a predominance of odd-chain free fatty acids. | 2014-07-10 |
20140193868 | EXTRACELLULAR POLYHYDROXYALKANOATES PRODUCED BY GENETICALLY ENGINEERED MICROORGANISMS - The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA). | 2014-07-10 |
20140193869 | CELL-FREE SYSTEM FOR CONVERTING METHANE INTO FUEL AND CHEMICAL COMPOUNDS - The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol. | 2014-07-10 |
20140193870 | EXTRACELLULAR ALDONOLACTONASE - The present disclosure relates to hydrolysis of hexose-δ-lactones by use of an | 2014-07-10 |
20140193871 | METHOD FOR ENHANCING CARBON BIOFIXATION - At least one product chosen from acids and alcohols is produced by a microbial fermentation process comprising: providing a gaseous substrate and a liquid medium to a microorganism, wherein the liquid medium comprises lactic acid and/or salts thereof, and wherein the gaseous substrate comprises at least one carbon source chosen from CO and CO | 2014-07-10 |
20140193872 | Process for the Conversion of Cellulosic Feedstock Materials - A process for converting a cellulosic feedstock to chemical compound, such as an alcohol, or mixture of chemical compounds, wherein one or more process streams can be used in one or more of the other process steps. | 2014-07-10 |
20140193873 | METHOD FOR PREPARING A HYDROCARBON - A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme. | 2014-07-10 |
20140193874 | NOVEL BIOMARKER FOR DIAGNOSIS OF LUNG CANCER - Disclosed are a composition for diagnosis of lung cancer including an antibody specific to GPI specific phospholipase D1 (GPLD1) protein, which is an effective biomarker for early diagnosis of lung cancer, and a composition for diagnosis of lung cancer including a primer or probe specific to a nucleic acid encoding the GPLD1 protein. The lung cancer diagnostic marker may be effectively used in early diagnosis of lung cancer and also, is very useful for evaluating progression of a disease and prognosis before and after treatment of the same. | 2014-07-10 |
20140193875 | POROUS POLYMERIC RESINS - Porous polymeric resins, reaction mixtures and methods that can be used to prepare the porous polymeric resins, and uses of the porous polymeric resin are described. More specifically, the polymeric resins typically have a hierarchical porous structure plus reactive groups that can be used to interact with or react with a variety of different target compounds. The reactive groups can be selected from an acidic group or a salt thereof, an amino group or salt thereof, a hydroxyl group, an azlactone group, a glycidyl group, or a combination thereof. | 2014-07-10 |
20140193876 | METHODS OF PURIFICATION OF NATIVE OR MUTANT FORMS OF DIPHTHERIA TOXIN - The present invention relates to the use of hydroxyapatite chromatography and multimodal chromatography, for punfication of diphtheria toxin, or a mutant form thereof, from a mixture, for example, a host cell fermentation mixture containing impurities such as host cell proteins and DNA. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of diphtheria toxin suitable for in vitro and in vivo applications. | 2014-07-10 |
20140193877 | THERMUS BROCKIANUS NUCLEIC ACID POLYMERASES - The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, | 2014-07-10 |
20140193878 | MRNA Interferase from Myxococcus Xanthus - A regulated deployment of a toxin gene for developmental programmed cell death in bacteria is described. | 2014-07-10 |
20140193879 | DFPASE ENZYMES FROM APLYSIA CALIFORNICA - The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-07-10 |
20140193880 | MODIFIED FACTOR VIII AND FACTOR IX GENES AND VECTORS FOR GENE THERAPY - The present invention relates to a modified and optimized Factor VIII or Factor IX nucleic acid for inclusion in a chimeric virus vector. Use of such vector can be used for treatment of hemophilia. | 2014-07-10 |
20140193881 | NOVEL ADAMTS-13 MUTANT - An enhanced disintegrin-like domain, and metalloprotease, with an isolated human thrombospondin type 1 motif, member 13 (ADAMTS-13) that includes substitutions at one or more positions in the isolated human ADAMTS-13. | 2014-07-10 |
20140193882 | NOVEL STRAINS OF BACTERIOPHAGES FOR THE TREATMENT OF BACTERIAL INFECTIONS, PARTICULARLY BY STRAINS OF DRUG-RESISTANT BACTERIA OF THE GENUS STENOTROPHOMONAS - Bacteriophage strains and their use in the production of preparations for use in the treatment of bacterial infections, particularly by strains of drug-resistant bacteria of the genus | 2014-07-10 |
20140193883 | GAS EXHAUST FILTER DEVICE FOR A BIOREACTOR - A gas exhaust filter device for a bioreactor with reduced blockage by condensed liquid is disclosed, which comprises a housing with a filter medium, a gas inlet and a gas outlet and also further comprises a plurality of ribs extending from at least one inner wall of the housing in a region between the gas inlet and the filter medium. | 2014-07-10 |
20140193884 | STEADY STATE ANAEROBIC DENITRIFYING CONSORTIUM FOR APPLICATION IN IN-SITU BIOREMEDIATION OF HYDROCARBON-CONTAMINATED SITES AND ENHANCED OIL RECOVERY - Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed. | 2014-07-10 |
20140193885 | PRODUCTION OF ITACONIC ACID - The invention relates to a nucleic acid sequence encoding an | 2014-07-10 |
20140193886 | SURFACTANTS THAT IMPROVE THE CLEANING OF LIPID-BASED STAINS TREATED WITH LIPASES - Described are compositions and methods relating to the removal of oily stains from fabrics and other surfaces using a lipase in combination with a selected surfactant to mediate the release of fatty acids generated by the lipase. The compositions and methods have application in, e.g., laundry cleaning and dishwashing. | 2014-07-10 |
20140193887 | Use of Secondary Paraffin Sulfonates for Increasing the Cleaning Capacity of Enzymes - An improvement for boosting the cleaning capacity of one or more enzymes includes providing one or more secondary paraffin-sulfonates to a cleaning composition. The method is particularly effective for textiles at low washing temperatures. | 2014-07-10 |
20140193888 | FABRIC AND HOME CARE PRODUCTS - This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN′ subtilisin and subtilisin derived from | 2014-07-10 |
20140193889 | ENZYMATIC ANTIMICROBIAL AND ANTIFOULING COATINGS AND POLYMERIC MATERIALS - Disclosed herein are a coating, a textile finish, a wax, elastomer, a filler, an adhesive, or a sealant, as well as polymeric materials such as a plastic, a laminate, a composite, that includes an enzyme that degrades cell wall or cell membrane components (e.g., a lysozyme, lytic transglycosylase) alone or in combination with other enzymes such as a lipolytic enzyme, a sulfuric ester hydrolase, an organophosphorus compound degradation enzyme, or an antimicrobial peptide. Also disclosed herein are methods of retarding or preventing microbial growth on or in a coating, paint, textile finish, wax, elastomer, adhesive, sealant, filler, or a polymeric material, where such a surface material includes an enzyme that degrades cell wall or cell membrane components (e.g., a lysozyme, lytic transglycosylase). | 2014-07-10 |
20140193890 | THERMOSTABLE ENZYMES AND METHODS OF MAKING AND USING THE SAME - Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes. Also provided herein are methods and compositions related to improved pectinolytic enzymes, such as pectate lyase, which exhibit enhanced activity, thermostability and/or longer half-life. | 2014-07-10 |
20140193891 | METHODS FOR DETECTION OF BIOLOGICAL SUBSTANCES - Methods and compositions are provided for detection of biological substances in nasal specimen. | 2014-07-10 |
20140193892 | IMAGE ANALYSIS AND MEASUREMENT OF BIOLOGICAL SAMPLES - Methods, devices, apparatus, and systems are provided for image analysis. Methods of image analysis may include observation, measurement, and analysis of images of biological and other samples; devices, apparatus, and systems provided herein are useful for observation, measurement, and analysis of images of such samples. The methods, devices, apparatus, and systems disclosed herein provide advantages over other methods, devices, apparatus, and systems. | 2014-07-10 |
20140193893 | GENETIC TEST SYSTEM - There is provided a genetic test system provided with a dispensing means for dispensing a sample and a reagent to a reaction vessel and a vessel conveyance means for conveying the reaction vessel. The system further includes a plurality of nucleic acid amplification detection units, each including a temperature control means for accommodating a plurality of reaction vessels and performing temperature control for each accommodated position of the reaction vessel, a temperature monitoring means for monitoring a value of temperature to be controlled of the reaction vessel, and a light emission measurement means for measuring light emission of reaction liquid in the reaction vessel. At least one of the nucleic acid amplification detection units individually has a vessel conveyance means different from the above-mentioned vessel conveyance means. | 2014-07-10 |
20140193894 | SENSOR AND PACKAGE - A fluid sensor comprises a formed plastic body and a reagent. The body has a top face with an integral first surface. The body also has a bottom face opposed to the first surface and a sidewall that extends from the periphery of the top face. The first surface is adapted to accept a fluid sample. The reagent is disposed on the integral first surface and causes a color change detectable on the bottom face when the reagent reacts with an analyte in the fluid sample. | 2014-07-10 |
20140193895 | AUTOMATED TISSUE ENGINEERING SYSTEM - The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings. | 2014-07-10 |
20140193896 | High Efficiency and High Precision Microfluidic Devices and Methods - New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available. | 2014-07-10 |
20140193897 | BIOREACTOR CONTAINER AND INTEGRITY CHECK METHOD FOR BIOREACTOR CONTAINERS - A bioreactor container comprising has an at least locally flexible wall and at least one container opening. The wall of the bioreactor container has a fluid-tight inner sheet, and an at least locally fluid-permeable or structured outer sheet. A method for testing the integrity of the bioreactor container also is provided. | 2014-07-10 |
20140193898 | SYSTEM FOR PRODUCING A BIOGAS - A method for producing a biogas is provided. The method includes the steps of providing a polyculture of aquatic plants to a growth system; continuously providing water, carbon dioxide, air and nutrients to the polyculture contained within the growth system; growing the polyculture for a time sufficient to produce an aquatic plant-based biomass; withdrawing a portion of the aquatic plant-based biomass contained within the growth system; and treating the withdrawn aquatic plant-based biomass to produce a biogas. | 2014-07-10 |
20140193899 | MONOCLONAL ANTIBODIES TO FIBROBLAST GROWTH FACTOR RECEPTOR 2 - The present invention is directed toward a monoclonal antibody to fibroblast growth factor receptor 2, a pharmaceutical composition comprising same, and methods of treatment comprising administering such a pharmaceutical composition to a patient. | 2014-07-10 |
20140193900 | DEVICE FOR WASHING SUSPENDED CELLS OR PARTICLES - A device and method are disclosed to enable fluid manipulations without disturbing or removing particles or cells. By engineering assay compartment geometries to take advantage of fluid mechanical effects, the flow velocity may be diminished around particles or cells. This technology may be used to process particles or cells of various kinds that are suspended in liquids or gels, and is suitable for use in high throughput screening. | 2014-07-10 |
20140193901 | LARGE SCALE CELL HARVESTING METHOD FOR PACK-BED CULTURE DEVICE - A large scale cell harvesting method for a pack-bed culture device by providing cell growing space that is loosely packed with a plurality of porous matrixes and/or matrix means. When a cell detaching step at least comprising shaking or spinning the culture chamber is performed, the unoccupied space is large enough for the porous matrixes collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrixes to harvest the cells. | 2014-07-10 |
20140193902 | PDX1 EXPRESSING ENDODERM - Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed. | 2014-07-10 |
20140193903 | Microcarriers for Stem Cell Culture - We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells. | 2014-07-10 |
20140193904 | PDX1 EXPRESSING ENDODERM - Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed. | 2014-07-10 |
20140193905 | EXPANSION OF DEFINITIVE ENDODERM CELLS - Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture. | 2014-07-10 |
20140193906 | SCREENING METHODS FOR SPINAL MUSCULAR ATROPHY - Disclosed herein are compositions and methods for treatment of spinal muscular atrophy (SMA). In certain embodiments, compounds are provided that increase full-length survival of motor neuron (SMN) protein production by an SMN2 gene. | 2014-07-10 |
20140193907 | PARTICLE BASED BIOLOGICALLY ACTIVE MOLECULE DELIVERY SYSTEMS - Described here are devices and methods for the delivery of molecules, including biologically active molecules, into tissues. The devices and methods are generally particle based, and devised to deploy the molecules into a target tissue in a controlled manner to minimize tissue damage. The devices and methods may further be devised to tailor molecule delivery parameters to a variety of tissue types. Kits including various device components are also described. | 2014-07-10 |
20140193908 | SEQUENTIAL ANTIBODY THERAPY - The invention provides a blocking ligand specific for CTLA-4 and a vascular targeting ligand/IL-2 complex, for sequential use in inhibiting the growth of tumour cell | 2014-07-10 |
20140193909 | Direct Differentiation of Cardiomyocytes From Embryonic Stem Cells - The present invention relates to the induction of differentiation in human embryonic stem (hES) cells to cardiomyocytes and factors such as prostaglandin I2 (PGI2), Fgf 9, Bmp6, Bmp4, Scf, Igf2, and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from hES cells is also provided wherein the media contains these factors. Genes that are upregulated in the process of cardiomyocyte differentiation are also provided. | 2014-07-10 |
20140193910 | METHODS FOR PRODUCING PANCREATIC PRECURSOR CELLS AND USES THEREOF - The present invention is directed to methods for readily propagating somatic pancreatic precursor cells. The methods comprise isolating cells from intact pancreatic samples and enhancing guanine nucleotide (GNP) biosynthesis in cultures comprising these cells, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the cells, thereby generating pancreatic precursor cells. | 2014-07-10 |
20140193911 | THERMORESPONSIVE CELL CULTURE SUPPORTS - The present invention relates to a cell culture support comprising a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex. | 2014-07-10 |
20140193912 | METHOD FOR INCREASING THE EFFICIENCY OF INDUCING PLURIPOTENT STEM CELLS - The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1a that modify histone. By utilizing Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission. | 2014-07-10 |
20140193913 | METHODS AND COMPOSITIONS FOR HOMOLOGOUS RECOMBINATION IN HUMAN CELLS - The methods and compositions described herein are based, in part, on the discovery of a stem cell state in human cells that resembles the morphology observed in murine-derived stem cells. Induction of such a state in human stem cells permits an increase in the efficiency of homologous recombination. Thus, the methods and compositions described herein relate to cells and methods for increasing the efficiency of homologous recombination in human stem cells. | 2014-07-10 |
20140193914 | Introduction of Modular Vector Elements During Production of a Lentivirus - Methods and kits for lentiviral production are provided. By separating certain components of the vector including but not limited to promoter, reporter and selection marker preference components from the genetic content of interest, one can create libraries that allow for the efficient generation of custom selected lentiviral vectors. | 2014-07-10 |
20140193915 | COMPOSITIONS AND METHODS FOR CUSTOM SITE-SPECIFIC DNA RECOMBINASES - The invention relates to biotechnology and provides novel compositions and methods for sequence-specific or sequence-directed transcription activator-like effector recombinases. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, and molecular breeding are also provided. | 2014-07-10 |
20140193916 | Electrotransformation of Clostridium pasteurianum - By this invention, for the first time, a method for high-efficiency genetic transformation of the anaerobic bacterium | 2014-07-10 |
20140193917 | MODULATION OF MEIOTIC RECOMBINATION - The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination. | 2014-07-10 |
20140193918 | AUTOMATIC ANALYZER - An automatic analyzer adapted to enhance sample/reagent dispensing accuracy, regardless of a difference in sample/reagent dispensing height of a dispensing probe. When the amount of sample in a sample container is small, tip height “h1” of a sample dispensing probe positioned immediately after it has aspirated the sample decreases below tip height “h′” of the sample dispensing probe positioned immediately before it discharges the sample. The sample in this state takes a concave shape at the tip of the sample dispensing probe positioned immediately before it discharges the sample. When the amount of sample in a sample container is large, tip height “h2” of the sample dispensing probe positioned immediately after it has aspirated the sample increases above the tip height “h′” and the sample takes a convex shape at the tip of the sample dispensing probe positioned immediately before it discharges the sample. | 2014-07-10 |
20140193919 | METHODS OF DETECTING OVARIAN CANCER - Methods of screening human female subject for a risk of ovarian cancer are described. High levels of calcium in serum are shown to be significantly positively associated with the risk of ovarian cancer and can advantageously be used to triage women into risk categories for more intensive testing. Also included are preoperative methods of determining if an adnexal mass is likely to be malignant or benign. | 2014-07-10 |
20140193920 | METABOLOMICS OF HUMAN BIOLOGICAL FLUIDS IDENTIFY SIGNATURES OF MALIGNANT GLIOMA - A method of identifying a patient in need of therapy to treat a malignant glioma comprising measuring a panel of polar metabolite levels in a biological sample taken from the patient and implementing a therapy to treat the malignant glioma in the patient. | 2014-07-10 |
20140193921 | TEST APPARATUS AND TARGET MEASUREMENT METHOD USING THE SAME - A test apparatus and method for measuring a concentration of a target by correcting for an impact of hemoglobin are provided. The target measurement method includes measuring an absorbance of hemoglobin in a sample, measuring an absorbance of a target in the sample, determining variation of the absorbance of the target according to the measured absorbance of the hemoglobin, and correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target. | 2014-07-10 |
20140193922 | Method for Combustion Analysis of Samples in a Combustion Analyzer - A method for combustion analysing a sample in a combustion analyzer ( | 2014-07-10 |
20140193923 | SENSORS AND METHODS FOR DETECTING PEROXIDE BASED EXPLOSIVES - Methods, compositions, and systems for detecting explosives is disclosed and described. A sensor for detecting explosives can comprise a porous hydrophilic material modified with a series of π conjugated molecules with the general structure: | 2014-07-10 |
20140193924 | INSTRUMENTS FOR MONITORING ELECTROSTATIC PHENOMENA IN REACTORS - Probes for monitoring electrostatic phenomena in challenging environments, such as fluidized bed reactors. These probes include a coated or uncoated static probe for measuring electric field and or particle charge state, an oscillatory electric field probe for measuring electric field, a chopped electric field probe for measuring electric field, and a radio-frequency antenna probe for detecting electrostatic discharges. | 2014-07-10 |
20140193925 | BIOSENSORS - A chemiresistive biosensor for detecting an analyte can include a high specific surface area substrate conformally coated with a conductive polymer, and a binding reagent immobilized on the conductive polymer, wherein the binding reagent has a specific affinity for the analyte. The conductive polymer can be deposited on a substrate by oCVD. | 2014-07-10 |
20140193926 | PYRENYLOXYSULFONIC ACID FLUORESCENT AGENTS - The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention. | 2014-07-10 |