28th week of 2011 patent applcation highlights part 42 |
Patent application number | Title | Published |
20110171635 | NUCLEIC ACID ENZYME BIOSENSORS FOR IONS - Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes. | 2011-07-14 |
20110171636 | MONO- AND MULTI-ELEMENT CODED LIBS ASSAYS AND METHODS - Methods for tagging an object with an element-coded particle and identifying the object based on the element code are described. LIBS analysis can be used with the methods to provide a high resolution system for identifying and quantifying objects with great specificity. Objects can include biological and chemical molecules. | 2011-07-14 |
20110171637 | METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS - The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among prostate cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients. | 2011-07-14 |
20110171638 | DIAGNOSIS OF FETAL ABNORMALITIES USING POLYMORPHISMS INCLUDING SHORT TANDEM REPEATS - The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample. | 2011-07-14 |
20110171639 | POLYMER FOR DETECTION OF TARGET SUBSTANCE, AND METHOD FOR DETECTION OF TARGET SUBSTANCE - Provided is a method of detecting a target substance by which the target substance can be detected with efficiency and a high sensitivity, and in a simple manner, a target substance detection polymer used in the method, and a method of forming the polymer. The method of detecting a target substance includes the steps of: (A) forming a target substance detection polymer by causing multiple kinds of nucleic acid probes for forming a polymer to react with a binding probe having a region capable of binding to the target substance and a region capable of binding to at least one of the nucleic acid probes in a solution; (B) binding the target substance detection polymer and the target substance; and (C) detecting the target substance detection polymer to which the target substance is bound. | 2011-07-14 |
20110171640 | Method for isolating cell free apoptotic or fetal nucleic acids - The present invention provides methods for isolating cell free nucleic acid, e.g., apoptotic or fetal nucleic acids and methods of detecting neoplastic cells or identifying the genetic composition of a fetus. The invention also provides magnetic particles comprising an anti-DNA antibody, and kits comprising the magnetic particles. | 2011-07-14 |
20110171641 | Gene Expression Markers of Recurrence Risk in Cancer Patients After Chemotherapy - The present invention relates to genes, the expression levels of which are correlated with likelihood of breast cancer recurrence in patients after tumor resection and chemotherapy. | 2011-07-14 |
20110171642 | Analysing Methylation Specific PCR by Amplicon Melting - The present invention provides a method of evaluating DNA methylation in a sample. The method comprises (i) reacting the DNA with an agent that differentially modifies methylated cytosine and non-methylated cytosine to produce modified DNA, (ii) amplifying the modified DNA by methylation specific PCR to produce amplified DNA, and (iii) subjecting the amplified DNA to melting analysis. In the method the methylation specific primers are selected such that the sequence between the primers includes a region of known sequence variation and/or at least one cytosine nucleotide. | 2011-07-14 |
20110171643 | METHOD, PROCESS, AND KIT FOR CANCER DIAGNOSIS, PROGNOSIS, AND AFTERCARE - A method or process for the diagnosis/prognosis/follow-up of cancer in vertebrates, comprising the following steps:
| 2011-07-14 |
20110171644 | Multiplex capture of nucleic acids - Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Methods of capturing one or more long nucleic acids and methods of capturing one or more nucleic acid for sequencing are also provided. Compositions, kits, and systems related to the methods are also described. | 2011-07-14 |
20110171645 | METHODS OF DIAGNOSING ACUTE CARDIAC ALLOGRAFT REJECTION - The present invention relates to methods of diagnosing acute rejection of a cardiac allograft using genomic expression profiling, proteomic expression profiling, metabolite profiling, or alloreactive T-cell genomic expression profiling, | 2011-07-14 |
20110171646 | MICRORNA EXPRESSION PROFILE ASSOCIATED WITH PANCREATIC CANCER - Methods are provided for diagnosing whether a subject has, or is at risk of developing, pancreatic cancer. The methods include measuring the level of at least one miR gene product in a biological sample derived from the subject's pancreas. An alteration in the level of the miR gene product in the biological sample as compared to the level of a corresponding miR gene product in a control sample, is indicative of the subject either having, or being at risk for developing, pancreatic cancer | 2011-07-14 |
20110171647 | METHOD FOR QUANTIFYING OR DETECTING DNA - The present invention relates to a method for quantifying or detecting DNA having a target DNA region, and so on. | 2011-07-14 |
20110171648 | METHOD OF DETECTING AND QUANTIFYING HEPATITIS C VIRUS - Methods, reagents, and kits for detecting hepatitis C virus (HCV) in biological samples. | 2011-07-14 |
20110171649 | DETECTION OF NUCLEIC ACIDS BY OLIGONUCLEOTIDE PROBES CLEAVED IN PRESENCE OF ENDONUCLEASE V - Particular aspects provide nucleic acid detection methods comprising contacting a test sample having a nucleic acid target sequence with at least one endo-V-cleavable oligonucleotide probe complementary to the target sequence in the presence of an endonuclease V, incubating the reaction mixture under conditions suitable to support hybridization of the endo-V-cleavable oligonucleotide probe with the target nucleic acid and endonuclease V-mediated cleavage of the target-hybridized probe, and detecting at least one endonuclease V-mediated cleavage product of the target-hybridized probe wherein the presence of the cleavage products is indicative of the presence of the target nucleic acid sequence in the sample. Particular aspects comprise amplification of the target nucleic acid sequence before and/or during the incubating and/or detecting, wherein detecting is post-amplification and/or real-time. Additional aspects provide suitable kits. Further aspects comprise use of at least one nick-directing modification or other structural modification of the at least one endo-V-cleavable oligonucleotide probe. | 2011-07-14 |
20110171650 | GENE EXPRESSION RELATED TO PREECLAMPSIA - Gene expression patterns contemporaneous with early placental development in the first trimester of preeclamptic versus unaffected pregnancies have been obtained. Observation of differences in these gene expression patterns has allowed the identification of biomarkers that are useful in predicting and monitoring preeclampsia. These biomarkers are also useful in screening potential therapeutics for efficacy in the prevention or treatment of preeclampsia. | 2011-07-14 |
20110171651 | METHOD FOR IDENTIFYING A NUCLEIC ACID IN A SAMPLE - A method of sample analysis is provided. In certain embodiments, the method may comprise: contacting a nucleic acid sample with a first primer and a second primer under PCR conditions to produce a double stranded product, wherein the second primer comprises a first label and is 5′ blocked; b) contacting the double stranded product with an exonuclease to degrade one strand of the double-stranded product to produce a single stranded product; c) contacting the single stranded product with a third primer under primer extension conditions, wherein the third primer comprises a second label; and d) detecting the first and second labels of the partial duplex. A kit for practicing the method is also provided. | 2011-07-14 |
20110171652 | CROSS PRIMINGAMPLIFICATION OF TARGET NUCLEIC ACIDS - Methods of amplifying target sequences by utilizing cross priming isothermal amplification are disclosed Methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence are also disclosed Reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases are also disclosed. | 2011-07-14 |
20110171653 | METHOD FOR DETECTING CRYPTOSPORIDIUM - A method for the detection and/or identification of | 2011-07-14 |
20110171654 | Allelic ladder loci - Disclosed are rare short tandem repeat (STR) alleles within the D10S1248 and D12S391 loci in humans. Provided are representative allelic ladders for each locus, methods and assays using these alleles and kits containing allelic ladders comprising these alleles for accurate genotyping and identification of a wide range of individuals. | 2011-07-14 |
20110171655 | PH MEASUREMENT FOR SEQUENCING OF DNA - The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dNTP) in the presence of appropriate reagents (DNA polymerase, and polymerase reaction buffer). Alternatively, or in addition, a change in pH resulting from the incorporation of nucleotides in the DNA polymerase reaction is measured. A device is provided having delivery channels for appropriate reagents, including dNTPs, which may be delivered sequentially or in a mixture. Preferably, the dNTPs are added in a predetermined sequence, and the dNTP is incorporated or not depending on the template sequence. | 2011-07-14 |
20110171656 | METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE - The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections—preferably on the inside of the vessel—in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface (“binding and normalizing step”) under high salt conditions, d) optionally washing (“washing step”), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method. | 2011-07-14 |
20110171657 | PROCESS FOR PREDICTING THE PROGNOSIS OF SQUAMOUS CELL LUNG CANCER - Disclosed in this specification is a method for predicting the prognosis of squamous cell lung cancer by observing regulatory changes in select miRNA sequences. These sequences may include hsa-mir-146b, hsa-mir-191, hsa-mir-206, hsa-mir-299-3p, hsa-mir-155, hsa-mir-15a, hsa-mir-122a, hsa-mir-513, hsa-mir-184, hsa-mir-511, hsa-mir-100, hsa-mir-10a, hsa-mir-453, hsa-mir-379, hsa-mir-202, hsa-mir-21, hsa-mir-126, hsa-mir-494, hsa-mir-432, hsa-mir-370, and combinations of these sequences. | 2011-07-14 |
20110171658 | METHOD OF QUANTIFYING POLYNUCLEOTIDES USING A STORED CALIBRATION CURVE - Method for quantifying analyte polynucleotide in a test sample using real-time amplification and adjustment of a stored calibration curve. The method may be practiced using as few as a single adjustment calibrator to adjust the stored curve. This simplifies the quantitative analysis procedure, while still providing the advantages of internal calibration adjustment to account for variation in amplification reaction efficiency. | 2011-07-14 |
20110171659 | Multipotent Adult Stem Cells, Sources Thereof, Methods of Obtaining and Maintaining Same, Methods of Differentiation Thereof, Methods of Use Thereof and Cells Derived Thereof - The present invention relates generally to mammalian multipotent adult stem cells (MASC), and more specifically to methods for obtaining, maintaining and differentiating MASC to cells of multiple tissue types. Uses of MASC in the therapeutic treatment of disease are also provided. | 2011-07-14 |
20110171660 | USE OF CCR9, CCL25/TECK, AND INTEGRIN ALPHA4 IN DIAGNOSIS AND TREATMENT OF MELANOMA METASTASIS IN THE SMALL INTESTINE - The invention relates to methods for determining whether a melanoma will metastasize or has metastasized to the small intestine in a subject by detecting or quantifying the expression of the CCR9, CCL25/TECK, or integrin α4 gene. Also disclosed are methods for treating subjects so identified. | 2011-07-14 |
20110171661 | METHOD FOR DIAGNOSIS OF POST-OPERATIVE RECURRENCE IN PATIENTS WITH HEPATOCELLULAR CARCINOMA - Provided are a method of diagnosing the recurrence or possibility of recurrence of hepa | 2011-07-14 |
20110171662 | NON-DENATURING LYSIS REAGENT - The invention provides a lysis reagent and method for preparing a test sample for use in an assay, wherein the method yields a homogeneous lysis mixture suitable for use in automated pipetting systems without the need for a centrifugation step. The lysis reagent includes a glycol and an alcohol. Other aspects of the invention include related immunoassays and test kits. | 2011-07-14 |
20110171663 | MICROTRENCH AND TUMOUR PROLIFERATION ASSAY - There is provided a cell culture microtrench being defined on or in a surface of a substrate, wherein the ratio of the width of the microtrench to the maximum length of the short axis of a cell type of interest is about 6 or preferably less, the length of the short axis of the cell type being measured when a cell is in detached or suspended form. There is also provided an array comprising such a microtrench and uses of such microtrenches, including cell-based assays. | 2011-07-14 |
20110171664 | METHODS AND REAGENTS FOR DETECTING SUSCEPTIBILITY TO GRAFT VERSUS HOST DISEASE OR TRANSPLANT RELATED MORTALITY - The present invention provides methods for determining the likelihood and/or severity of GvHD and the likelihood of the occurrence of transplant related mortality. | 2011-07-14 |
20110171665 | P-SELECTIN ASSOCIATED WITH EOSINOPHILS AS A MARKER FOR ASTHMA AND CORRELATING WITH B-1 INTEGRIN ACTIVATION - Methods are provided for the detection of P-selectin associated with eosinophils and for the use of P-selectin as a biological marker for asthma. In one embodiment, the present invention relates to methods for detecting P-selectin in a sample containing eosinophils and quantifying the total number of eosinophils. In another embodiment, the present invention relates a method for determining the proportion of eosinophils that are P-selectin positive and positive for at least partially activated β-1 integrin. In yet another embodiment, the present invention relates to kits for the detection of P-selectin and for the detection of eosinophils that are both P-selectin positive and positive for at least partially activated β-1 integrin. In still yet another embodiment, the invention relates to a method for monitoring a biological condition. | 2011-07-14 |
20110171666 | PEPTIDE-LIPID CONSTRUCTS AND THEIR USE IN DIAGNOSTIC AND THERAPEUTIC APPLICATIONS - Peptide-lipid constructs of the structure L-S—F are disclosed, where F is a peptide, S is a spacer covalently linking F to L via an oligomer of ethylene glycol, and L is a diacyl- or dialkyl-glycerolipid (including glycerophospholipids). The spacer ideally has 6 to 14 ethylene glycol repeats, corresponding to PEG with a molecular weight of approximately 250 to 600. Also disclosed is a method of detecting reactive antibodies in serum by contacting serum with cells modified to incorporate a peptide-lipid construct, where the peptide is an epitope of the antibody, and determining the degree of aggluthination of the cells. | 2011-07-14 |
20110171667 | REACTION MEDIUM FOR DETECTING AND/OR IDENTIFYING BACTERIA OF THE LEGIONELLA GENUS - A reaction medium for the culture and/or detection and/or identification of bacteria of the | 2011-07-14 |
20110171668 | Determination Of Equilibrium Constants In Solution By Multi-Step Kinetics - The invention relates to a method for determining a first equilibrium constant of a first equilibrium reaction via MSK analysis of a second equilibrium reaction, whereby the first and the second equilibrium reaction share at least one reactant. | 2011-07-14 |
20110171669 | Isolated luciferase gene of L. ITALICA - The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of | 2011-07-14 |
20110171670 | REAGENT OPEN MECHANISM OF LUMINESCENCE MEASUREMENT SYSTEM AND OPEN NEEDLE CONTROL METHOD IN REAGENT OPEN MECHANISM - A reagent open mechanism of the luminescence measurement system comprises a triaxial actuator and a reagent dispensing nozzle which is driven by the triaxial actuator. A reagent cartridge where a reagent to be divided by the reagent dispensing nozzle is filled in a concave and the opening of the concave is sealed by an aluminum sheet can be set in. This reagent open mechanism comprises an open needle which is driven by the triaxial actuator and makes a hole in the aluminum sheet and a fixation block between the reagent dispensing nozzle and the open needle which arranges the reagent dispensing nozzle and the open needle in such location that the reagent dispensing nozzle or the open needle does not contact with a structure including the reagent cartridge in a Z-axis operation during opening time or reagent dividing and dispensing time. | 2011-07-14 |
20110171671 | METHOD FOR DIAGNOSING ACUTE CARDIAC ISCHEMIA - Chemiluminescent detection of metabolic by-products of inosine and hypoxanthine is used to diagnose ischemic events such as early acute cardiac ischemia. | 2011-07-14 |
20110171672 | METHOD OF REDUCING MEASUREMENT ERROR CAUSED BY CATALASE INHIBITION BY AZIDE - Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured. | 2011-07-14 |
20110171673 | COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 2011-07-14 |
20110171674 | Beta-Glucosidase Variants Having Improved Activity, and Uses Thereof - The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of | 2011-07-14 |
20110171675 | FRET-BASED MEMBRANE-TYPE 1 MATRIX METALLOPROTEINASE BIOSENSOR AND METHODS FOR USING THE SAME - The present invention is a novel FRET-based biosensor composed of ECFP and YPet fluorescent proteins operably linked via a MT1-MMP recognition sequence for use in the detection of cancer cells in a biological sample. | 2011-07-14 |
20110171676 | ASC AS A MARKER FOR LUNG CANCER - The present invention relates to the assessment of lung cancer. It discloses the use of protein ASC in the assessment of lung cancer. It also relates to a method for assessing lung cancer in vitro using a liquid sample, derived from an individual by measuring ASC in the sample. Measurement of ASC can, e.g., be used in the early detection or in the follow-up of patients with lung cancer. | 2011-07-14 |
20110171677 | FLUORESCENCE DETECTION OF POISON OAK OIL - The invention herein disclosed provides for compositions, methods for synthesizing said compositions, and methods for using said compositions, wherein the compositions and methods may be used to bind to and/or deactivate a poison oak oil, such as urushiol. The compositions and methods can be used to treat and/or reduce an inflammatory reaction and/or hypersensitivity to natural compounds found in poison oak, poison ivy, poison sumac, mango, lac tree, and cashew nut. | 2011-07-14 |
20110171678 | MODIFIED CARBOCYANINE DYES AND THEIR CONJUGATES - Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens. | 2011-07-14 |
20110171679 | L-GLUTAMIC ACID-PRODUCING MICROORGANISM AND A METHOD FOR PRODUCING L-GLUTAMIC ACID - A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells. | 2011-07-14 |
20110171680 | BUFFY COAT TUBE AND FLOAT SYSTEM AND METHOD - A tube and float system for use in separation and axial expansion of the buffy coat is provided. The system includes a transparent, or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The sample tube has an elongated sidewall having a first cross-sectional inner diameter. The float consists of a main body portion and one or more support members protruding from the main body portion to engage and support the sidewall of the sample tube. The main body portion and the support members of the float have a cross-sectional diameter less than that of the first cross-sectional inner diameter of the tube when the sample tube is expanded, such as by centrifugation. The main body portion of the float together with an axially aligned portion of the sidewall define an annular volume therebetween. The support members protruding from the main body portion of the float traverse said annular volume to produce one or more analysis areas. During centrifugation, the centrifugal force enlarges the diameter of the tube to permit density-based axial movement of the float in the tube. Thereafter, the centrifugal force is reduced to cause the tube sidewall to return to its first diameter, thereby capturing the float and trapping the buffy coat constituents in the analysis area. The bully coat constituents can then be evaluated or measured. | 2011-07-14 |
20110171681 | METHODS AND COMPOSITIONS FOR REGULATION OF STEM CELL SURVIVAL, PROLIFERATION, AND DIFFERENTIATION BY PROTEIN UBIQUITINATION - Compositions and methods for regulating in vitro cell growth are disclosed, and for providing undifferentiated stem cells or embryonic cells that are suitable for transplantation into damaged tissues or organs, or for use in tissue repair. A representative method includes causing the overexpression or underexpression of GalT binding protein (GtBP), also referred to as GalT associated protein (GTAP), in a cell such that ubiquitination of at least one cellular protein associated with cell adhesion and/or cell-to-cell interaction is correspondingly increased or decreased, causing inhibition of cell growth when GTAP is overexpressed and causing enhanced cell growth when GTAP is underexpressed by the cell. As a result, growth of the cell is altered or regulated. | 2011-07-14 |
20110171682 | SCREENING METHODS EMPLOYING INSECTS WITH BLOOD BRAIN BARRIER - There is provided insect models that are aimed to reflect vertebrate blood-brain barrier (BBB) penetration. Investigation of BBB penetration is extremely important in drug discovery; successful CNS drugs have to cross the BBB, while BBB penetration may cause unwanted side effects for peripheral acting drugs. Specifically, the present invention relates to the use of insects in screening for substances with a biological effect on the brain or central nervous system and/or effect on a disease or disorder of the brain or central nervous system. It further relates to use of such insects in screening for substances that have a desired biological activity and which do not cross the blood brain barrier. | 2011-07-14 |
20110171683 | METHODS AND COMPOSITIONS FOR ENUMERATING ANTIBIOTIC-RESISTANT MICROORGANISMS - A thin film culture device for detection of antibiotic-resistant microorganisms is provided. The device can include indicators to differentiate staphylococcal from non-staphylococcal microorganisms. Methods of use include detecting or enumerating antibiotic-resistant microorganisms. The methods further include obtaining a differential count of staphylococcal and non-staphylococcal microorganisms. | 2011-07-14 |
20110171684 | INSPECTION METHOD OF AIRBORNE FLOATING BACTERIA AND ITS APPARATUS - Inspection arrangements of airborne floating bacteria for performing a biological light emission reaction by using an ATP light emission reagent on the basis of the ATP derived from viable cells in captured airborne floating bacteria in the air contained in the inspection sample, measuring the light emission quantity due to the biological light emission reaction, determining the ATP quantity contained in the inspection sample, and counting the number of cells in the airborne floating bacteria, the light emission quantity of the ATP light emission reagent is measured, and a measured value of light emission quantity of the ATP light emission reagent and a predetermined theoretical value corresponding to the measured value are compared, and thereby the reliability of the ATP light emission reagent to be used and the inspection results is evaluated at the same time. | 2011-07-14 |
20110171685 | Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Paper Mill Sludge - A method for producing a fermentation product from a lignocellulose-containing material comprises pre-treating the lignocellulose-containing material; introducing paper mill sludge to the pre-treated lignocellulose-containing material; exposing the pre-treated lignocellulose-containing material to an effective amount of hydrolyzing enzyme; and adding a fermenting organism to produce a fermentation product. In an embodiment, the paper mill sludge comprises deinking paper sludge. | 2011-07-14 |
20110171686 | REVERSE CUMATE REPRESSOR MUTANT - Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from | 2011-07-14 |
20110171687 | UNSTRUCTURED RECOMBINANT POLYMERS AND COMPOSITIONS COMPRISING SAME - The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications. | 2011-07-14 |
20110171688 | METHOD OF PROVIDING IMMUNOGLOBULIN SECRETING B LYMPHOCYTES AND HUMAN ANTIBODIES - Provided is a method for producing a clone of an immortalized human B memory lymphocyte, comprising the step of inducing or enhancing telomerase activity in the B lymphocyte in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalized human B memory lymphocyte capable of producing a human monoclonal antibody with desired antigen specificity. | 2011-07-14 |
20110171689 | ARTIFICIAL IMMUNE SYSTEM: METHODS FOR MAKING AND USE - The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system. | 2011-07-14 |
20110171690 | ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE - To provide ACE inhibitory peptides which can effectively inhibit ACE by a small amount of ingestion and have no fear of causing side effects and which can be orally ingested easily during daily life by persons having high blood pressure, and compositions comprising the peptides. The peptides represented by the following structural formulae (1) to (9), and salts thereof are provided. (1) Asp-Arg-Pro, (2) Asn-Trp, (3) Val-Gly-Leu, (4) Ile-Gly-Val, (5) Gly-Val-Pro, (6) Ile-Pro-Tyr, (7) pyroGlu-Pro, (8) Tyr-Thr, (9) Pro-Trp | 2011-07-14 |
20110171691 | GENETICALLY MODIFIED STRAINS FOR BIOTRANSFORMATIONS IN ANTHRACYCLINE PRODUCTION - A microbial strain which converts anthracycline metabolites into non-natural anthracycline antibiotics is described. A process for converting anthracycline metabolites into anthracycline antibiotics using a microbial strain is also described. | 2011-07-14 |
20110171692 | PROCESS FOR AMPLIFYING DNA IN CELLS - The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii):
| 2011-07-14 |
20110171693 | AMYLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. | 2011-07-14 |
20110171694 | Alpha-Amylase Mutants with Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca | 2011-07-14 |
20110171695 | ALLELES OF THE PRPD1 GENE FROM CORYNEFORM BACTERIA - An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described. | 2011-07-14 |
20110171696 | Biological Synthesis of Difunctional Alkanes from Carbohydrate Feedstocks - Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid. | 2011-07-14 |
20110171697 | CATALYZED PREPARATION OF (METH)ACRYLATES OF N-HYDROXYALKYLATED AMIDES - The claimed invention relates to a process for preparing (meth)acrylates of N-hydroxyalkylated amides, in which open-chain N-hydroxyalkylated amides are esterified with (meth)acrylic acid or transesterified with at least one (meth)acrylic ester in the presence of at least one heterogeneous catalyst selected from the group consisting of an inorganic salt and an enzyme. | 2011-07-14 |
20110171698 | C-22 HYDROXYLASE - β-Amyrin, a precursor in biosynthesis of soyasapogenol B, is biosynthesized by cyclization of 2,3-oxidosqualene which is generated by the mevalonate pathway, and soyasapogenol B is biosynthesized by two hydroxylations of β-amyrin. However, a gene of 22-hydroxylase involved in the sequence of reactions has not been identified. The present inventors identified a gene encoding the hydroxylase for oleanene triterpenes at C-22, and found that oleanene triterpenes could be hydroxylated at C-22 by co-expressing this gene together with one or more specific genes. Further, the present inventors found that soyasapogenol B could be efficiently produced in large quantities by co-expressing this gene for 22-hydroxylase with a gene for 24-hydroxylase. | 2011-07-14 |
20110171699 | PREPARATION OF 6-AMINOCAPROIC ACID FROM 5 -FORMYL VALERI C ACID - The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam. | 2011-07-14 |
20110171700 | METHOD FOR PRODUCING L-PHENYLEPHRINE USING AN ALCOHOL DEHYDROGENASE OF AROMATOLEUM AROMATICUM EBN1 (AZOARCUS SP. EBN1) - The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol. | 2011-07-14 |
20110171701 | METHOD FOR PRODUCING ACRYLAMIDE - [Problem] The present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst with high productivity at low cost, in which reaction heat can be efficiently removed even at an industrial reaction scale. | 2011-07-14 |
20110171702 | RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID - The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor. | 2011-07-14 |
20110171703 | BACTERIUM CAPABLE OF PRODUCING LACTIC ACID, AND METHOD FOR PRODUCING LACTIC ACID - The present invention provides: a lactic acid-producing | 2011-07-14 |
20110171704 | METHOD FOR PRODUCING LACTIC ACID FROM PLANT-DERIVED RAW MATERIAL, AND LACTIC-ACID-PRODUCING BACTERIUM - The present invention provides: a lactic acid-producing | 2011-07-14 |
20110171705 | PROCESS FOR THE PRODUCTION OF ALCOHOL - The invention provides a process for the production of an alcohol which comprises cleaving polysaccharides in a cellulosic material in an aqueous medium at a temperature of at least 45° C. using a thermophilic microorganism to yield fermentable sugars, fermenting an aqueous solution of said sugars at a temperature of at least 45° C. with a thermophilic microorganism to yield an alcohol or an alkanoate, if necessary reducing said alkanoate to yield an alcohol, and removing said alcohol from said aqueous solution. | 2011-07-14 |
20110171706 | Integrated Process for Production of Alcohol from Syngas and Removal of CO2 - A process for producing alcohol from syngas integrates the alcohol recovery with the removal of CO | 2011-07-14 |
20110171707 | Composition and Methods for Improving the Production of Fermentation Operations - Methods and compositions for improving the production of ethanol by a microorganism in a fermentation medium are provided, where the method comprises adding to the fermentation medium an emulsion comprising a monoterpene and a surfactant in an amount sufficient to improve the production of ethanol. | 2011-07-14 |
20110171708 | Non-sterile fermentation of bioethanol. - A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-bacterial treatment for first generation starch fermentations. | 2011-07-14 |
20110171709 | Product Recovery From Fermentation of Lignocellulosic Biomass - The present invention is directed to a process of producing ethanol from lignocellulosic biomass, which comprises pre-treating a lignocellulosic feedstock to produce a reactive carbohydrate mixture; adding activated carbon in free form; converting said reactive carbohydrate mixture to form a beer; separating solids from said carbohydrate mixture or said beer or both, wherein said activated carbon is separated along with the solids in said mixture, said beer or both; and drying said solids. The invention is also directed to the production of a dried solid fuel to be combusted during said process. | 2011-07-14 |
20110171710 | METHOD FOR PRODUCING CELLULOSIC ETHANOL - A method for producing cellulosic ethanol is disclosed, which comprises the following steps: adding the medium containing the source materials of cellulose and/or hemicellulose to fermentation reaction vessel; adding cellulase to fermentation reaction vessel, and inoculating | 2011-07-14 |
20110171711 | Methods and Compositions for Production of Methane Gas - The present invention provides methods and compositions for sustained methane production from atmospheric CO | 2011-07-14 |
20110171712 | SELF-ASSEMBLING TISSUE MODULES - The invention relates to a new approach to constructing cellular aggregates in vitro and their use in methods for producing 3D-tissue constructs in a modular way. | 2011-07-14 |
20110171713 | SORBENT FOR A DIALYSIS DEVICE - There is provided a sorbent for removing metabolic waste products from a dialysis liquid, the sorbent comprising a layer of immobilized uremic toxin-treating enzyme particles intermixed with cation exchange particles. | 2011-07-14 |
20110171714 | CRYSTAL STRUCTURE OF TAK1-TAB1 - The invention relates to molecules or molecular complexes which comprise binding pockets of TAK1 or its structural homologues. The invention relates to crystallizable compositions and crystals comprising TAK1. The present invention also relates to a data storage medium encoded with the structural coordinates of molecules and molecular complexes which comprise the TAK1 or TAK1-like ATP-binding pockets. The present invention also relates to a computer comprising such data storage material. The computer may generate a three-dimensional structure or graphical three-dimensional representation of such molecules or molecular complexes. This invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. In addition, this invention relates to methods of using the structure coordinates to screen for and design compounds, including inhibitory compounds, that bind to TAK1 or homologues thereof. | 2011-07-14 |
20110171715 | BIOCOMPATIBLE POLYMER AND MAGNETIC NANOPARTICLE WITH BIOCOMPATIBILITY - The invention discloses a biocompatible polymer for covalently modifying magnetic nanoparticles. The biocompatible polymer may be coupled to a targeting agent and/or a fluorescent dye. The invention also discloses a magnetic nanoparticle with biocompatibilities comprising the biocompatible polymer. | 2011-07-14 |
20110171716 | CARBOHYDRATE-BASED DRUG DELIVERY POLYMERS AND CONJUGATES THEREOF - Provided herein are water-soluble carbohydrate polymers which are monoderivatized at their reducing terminus, such that the carbohydrate polymers can be selectively conjugated at a single location. Also provided are methods of preparation and conjugation of the monoderivatized carbohydrate polymers. | 2011-07-14 |
20110171717 | METHOD FOR ENHANCING POLYMERASE ACTIVITY - A polymerase activity is effectively enhanced by adding an anionic surfactant, in particular an anionic surfactant having a polyethoxyl group, to a reaction mixture containing a polymerase. | 2011-07-14 |
20110171718 | Proteases With Modified Pre-Pro Regions - The invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the modified polynucleotides comprise one or more mutations that encode modified proteases having modifications of the pre-pro region that enhance the production of the active enzyme. The present invention further relates to methods for altering the production of proteases in microorganisms, such as | 2011-07-14 |
20110171719 | Prevention and Remediation of Petroleum Reservoir Souring and Corrosion by Treatment with Virulent Bacteriophage - Petroleum reservoir souring, caused by microbially induced production of hydrogen sulfide and other sulfur compounds, and the attendant corrosion are remediated by isolating bacteriophage(s) specific for the problematic bacteria (target bacteria) and adding an effective amount of such bacteriophage(s) to water introduced into or resident in the reservoir to kill at least some of the target bacteria. Suitable virulent bacteriophage(s) may be indigenous to the water, located in surrounding areas, or taken from a known banked stock. Means of concentrating solutions of bacteriophage(s) are also disclosed. | 2011-07-14 |
20110171720 | SINGLE-DOMAIN BRAIN-TARGETING ANTIBODY FRAGMENTS DERIVED FROM LLAMA ANTIBODIES - A phage-displayed library of llama single heavy domain antibodies (sdAbs) was enriched for species that selectively bind to and are internalized by human cerebromicrovascular endothelial cells (HCEC). From the enriched library, two sdAbs were selected, sequenced, subcloned, and expressed as fusion proteins with c-myc-His | 2011-07-14 |
20110171721 | Targeted Gene Deletions for Polysaccharide Slime Formers - The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R | 2011-07-14 |
20110171722 | Bacillus Licheniformis and Method for Detoxification of Zearalenone - The present invention discloses an isolated pure culture of a novel strain CK1 of | 2011-07-14 |
20110171723 | System and Method For Biogeochemical Stabilization of Chromate-Impacted Solids, Including Chromite Ore Processing Residue (COPR) - The invention relates to a system and method for the substantially permanent biogeochemical stabilization of solids impacted with hexavalent chromium/Cr(VI), including chromite ore processing residue (“COPR”). The invention comprises a novel treatment method of adding amendment(s) to COPR or other chromate impacted solids for the purpose of (1) weathering COPR minerals (when present) to convert the minerals that control alkalinity of the COPR to non-alkaline forms and liberate incorporated hexavalent chromium (Cr(VI)) in the process; (2) providing a chemical reductant (ferrous iron) to rapidly and permanently reduce the available Cr(VI) to trivalent form (Cr(III)); and/or (3) supporting longer-term biogeochemical Cr(VI) reduction enhanced by recycling of the chemical reductant, ultimately rendering the material non-hazardous as measured by acceptable methods. Amendments include but are not limited to acids; sources of ferrous iron; fermentable organic carbon source(s); and/or a source of active anaerobic microbes. | 2011-07-14 |
20110171724 | Apparatus and Method for Removing Microbial Contaminants From a Flowing Fluid - Methods and apparatuses for removing microbial contaminants from a flowing fluid in a cell culture incubator are disclosed. Some embodiments of the invention provide a cell culture incubator including a chamber, an airflow passage through which gasses circulate within the chamber, a filter configured to filter gasses that flow through the airflow passage and chamber, and a blower for circulating gasses through the airflow passage, chamber and filter. The blower includes a structural component at least partially formed from an anti-microbial material. | 2011-07-14 |
20110171725 | MECHANISM AND METHOD OF PREVENTING SUCTION AIR FROM LEAKING DURING FILTRATION OF CAPTURING CARRIER SOLUTION - A mechanism for preventing suction air from leaking uses a capturing carrier cartridge that includes a cartridge main body and a filter fixing ring. The cartridge main body includes a storage portion for storing a capturing carrier solution and a filter mounting portion where a filter for filtering the capturing carrier solution is disposed. The filter fixing ring covers the filter mounting portion, positions the filter, and sucks and filters the capturing carrier solution from a lower surface thereof. The mechanism includes a cartridge holder and a suction head. The cartridge holder includes an opening that includes the filter mounting portion of the capturing carrier cartridge, and a holding portion that holds the storage portion. The suction head sucks the capturing carrier solution through the lower surface of the filter fixing ring while applying pressure to press the filter fixing ring against the filter mounting portion. | 2011-07-14 |
20110171726 | MULTIPOTENT STEM CELLS DERIVED FROM HUMAN ADIPOSE TISSUE AND CELLULAR THERAPEUTIC AGENTS COMPRISING THE SAME - Human adipose tissue-derived multipotent adult stem cells are provided, which are characterized by the ability to be maintained in an undifferentiated state for a long period of time by forming spheres and which have high proliferation rates. Also provided are methods for isolating and maintaining the adult stem cells, and methods for differentiating the multipotent adult stem cells into nerve cells, fat cells, cartilage cells, osteogenic cells, muscle cells, endothelial cells, hepatic cells and insulin-releasing pancreatic β-cells. Also provided are cellular therapeutic agents for treating osteoarthritis, osteoporosis, nerve disease, diabetes and for forming breast tissue, which contain differentiated cells or the adult stem cells. | 2011-07-14 |
20110171727 | COMPOSITIONS AND METHODS FOR ACTIVATING GENES OF INTEREST - Compositions and methods for activating genes of interest are provided. The compositions comprise a masked targeted expression cassette which expresses a gene product only in the presence of a target molecule. The cassettes are useful for the treatment of disease and for preventing the proliferation of neoplastic cells. | 2011-07-14 |
20110171728 | Method and composition for crystallizing G protein-coupled receptors - Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR. | 2011-07-14 |
20110171729 | Method for Producing Stable Mammalian Cell Lines Producing High Levels of Recombinant Proteins - The invention provides a novel method for generating stable mammalian cell lines with enhanced protein production capabilities, and to expression vectors and related methods for high level expression of biopharmaceutical proteins of interest. | 2011-07-14 |
20110171730 | Conjugates For Use In Hepatocyte Free Uptake Assays - The present invention provides methods of identifying oligomeric compounds, such as siRNA and double-stranded RNA compounds, having bioactivity in vivo, and kits. | 2011-07-14 |
20110171731 | COMPOSITIONS CONTAINING PLATELET CONTENTS - This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided. | 2011-07-14 |
20110171732 | HIGHLY POROUS SOLID MATERIAL MADE OF BIODEGRADABLE POLYMER AND METHOD OF FABRICATING, PROCESSING, AND CELL-SEEDING THE SAME - [Problems] A purpose of the invention is to provide highly porous 3D scaffolds made of biodegradable polymer such as poly-lactic acid, which can be preferably used for cell culture. | 2011-07-14 |
20110171733 | Bovine Immunodeficiency Virus (BIV) Based Vectors - BIV packaging constructs, BIV packaging cell lines, methods of making BIV packaging cells and methods of making BIV producer cells are described. | 2011-07-14 |
20110171734 | CONJUGATIVE PLASMIDS AND METHODS OF USE THEREOF - The present invention provides novel | 2011-07-14 |