30th week of 2020 patent applcation highlights part 27 |
Patent application number | Title | Published |
20200231950 | NOVEL MUTATED PTE ENZYMES - Disclosed are mutated phosphotriesterase enzymes with improved stability and activity, as well as their use in particular for degrading organophosphorus compounds. | 2020-07-23 |
20200231951 | COMPOSITION FOR DETECTING ALPHA PARTICLE RADIATION AND METHODS OF USE - A capsule composition comprising: (a) a polyester shell having a thickness of no more than 20 microns, and (b) a solution containing a visual and/or olfactory indicator, wherein the solution is encapsulated by the polyester shell. Also described herein is a method for detecting alpha particle radiation, in which: (i) the capsule composition is placed in contact with an esterase in a location where the presence of alpha particle radiation is being determined; (ii) waiting a period of time for the esterase to degrade the polyester shells, wherein the period of time is insufficient for the esterase to cause leakage of the solution in the absence of alpha particle radiation but is sufficient for alpha particle radiation, if present, to cause leakage from the capsule composition; and (iii) observing whether leakage has occurred at the end of the period of time to determine whether alpha particle radiation is present. | 2020-07-23 |
20200231952 | MATERIALS AND METHODS FOR INCREASING GENE EDITING FREQUENCY - Materials and methods for gene editing using improved targeted endonucleases and endonuclease systems (e.g., Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) endonuclease systems) are provided herein. | 2020-07-23 |
20200231953 | COMPOSITIONS AND METHODS FOR TRANSIENT DELIVERY OF NUCLEASES - The disclosure in some aspects relates to recombinant adeno-associated viruses having nuclease grafted to one or more capsid proteins. In some aspects, the disclosure relates to isolated AA V capsid proteins having terminally grafted nucleases and isolated nucleic acids encoding the same. Recent approaches to delivering nucleases to cells for gene editing have focused on delivering of expression vectors engineered to express the nucleases in target cells. However, these approaches have proved to be problematic in many instances due to genotoxicity resulting from to prolonged expression of gene editing system in vivo. To prevent such off-target genotoxicity due to prolonged presence of a gene editing system, several studies explored delivery of mRNA or protein instead of delivering the gene coding for the nucleases in cell culture. | 2020-07-23 |
20200231954 | GENE THERAPIES FOR LYSOSOMAL DISORDERS - The disclosure relates, in some aspects, to compositions and methods for treatment of diseases associated with aberrant lysosomal function, for example Parkinson's disease and Gaucher disease. In some embodiments, the disclosure provides expression constructs comprising a transgene encoding beta-Glucocerebrosidase (GBA) or a portion thereof, Lysosomal Membrane Protein 2 (LIMP2), Prosaposin, or any combination of the foregoing. In some embodiments, the disclosure provides methods of Parkinson's disease by administering such expression constructs to a subject in need thereof. | 2020-07-23 |
20200231955 | ENDOGLUCANASE COMPOSITIONS AND METHODS - The invention is directed to novel variant endoglucanases. | 2020-07-23 |
20200231956 | MEANS AND METHODS FOR SINGLE MOLECULE PEPTIDE SEQUENCING - The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction. | 2020-07-23 |
20200231957 | PERFORMANCE-ENHANCED PROTEASE VARIANTS II - An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) amino acid substitutions at the positions corresponding to the positions 9 and 271 in each case based on the numbering according to SEQ ID No. 1, and (b) an amino acid substitution on at least one of the positions corresponding to the positions 29, 48, 101, 30, 131, 133, 144, 224 or 252, in each case based on the numbering according to SEQ ID No. 1. Such proteases are particularly suitable for having an improved cleaning performance. | 2020-07-23 |
20200231958 | FACTOR IX POLYPEPTIDE MUTANT, ITS USES AND A METHOD FOR ITS PRODUCTION - Disclosed are a modified FIX (Factor IX) polypeptide comprising a leucine, cysteine, aspartic acid, glutamic acid, histidine, lysine, asparagine, glutamine or tyrosine in position 338; pharmaceutical preparations containing said modified FIX polypeptide; a nucleotide sequence coding for the modified FIX polypeptide; and a method for producing the modified FIX polypeptide. | 2020-07-23 |
20200231959 | ENGINEERED PHENYLALANINE AMMONIA LYASE POLYPEPTIDES - The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides. | 2020-07-23 |
20200231960 | CELL OR TISSUE EMBEDDING DEVICE - To provide a cell or tissue embedding device highly capable of supplying a physiologically active substance, by curbing the reduction of living cells or living tissue in the process of preparing a PVA gel containing the living cells or living tissue. | 2020-07-23 |
20200231961 | Device For Facilitating The Isolation Of Targets From A Biological Sample - A method and a device are provided for facilitating the disaggregation of a plurality of solid phase substrate as the plurality of solid phase substrate are transferred from a first location to a second location. A plurality of solid phase substrate are deposited at the first location and a plurality of particles are added to the plurality of solid phase substrate. A force is generated to draw the plurality of solid phase substrate from the first location to a second location. The plurality of particles causes clumps of the plurality of solid phase substrate to break apart as the plurality of solid phase substrate are moved in response to the force. | 2020-07-23 |
20200231962 | DISPLAY LIBRARY PROCESS - Disclosed are methods for identifying desired members from a display libraries, including bacteriophage display libraries. Display library members can be amplified in the presence of a target compound so that cycles of selection can be rapidly completed. | 2020-07-23 |
20200231963 | POLYPEPTIDE LIBRARY - The invention relates to novel polypeptide libraries that are conformationally constrained in an anti-parallel, helix-turn-helix arrangement. The invention further relates to methods of generating and screening such libraries for biological, pharmaceutical and other uses. | 2020-07-23 |
20200231964 | TAGGING NUCLEIC ACID MOLECULES FROM SINGLE CELLS FOR PHASED SEQUENCING - The present disclosure provides methods for long-read sequencing from single cells. The method can comprise constructing a nucleic acid library and reconstructing longer nucleic acid sequences by clustering and assembling a plurality of shorter nucleic acid sequences. | 2020-07-23 |
20200231965 | Treatment Of Ophthalmic Conditions With Angiopoietin-Like 7 (ANGPTL7) Inhibitors - The present disclosure provides methods of treating patients having an ophthalmic condition, methods of identifying subjects having an increased risk of developing an ophthalmic condition, methods of detecting human angiopoietin like 7 (ANGPTL7) variant nucleic acid molecules and variant polypeptides, and ANGPTL7 variant nucleic acid molecules and variant polypeptides. | 2020-07-23 |
20200231966 | AGENTS USEFUL IN TREATING FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY - Antisense agents and RNA interference agents useful for treating diseases and conditions the treatment of which can benefit from reducing the expression of double homeobox 4 and/or double homeobox 4c, more particularly facioscapulohumeral muscular dystrophy. Methods, uses and further products employing such agents are also described. | 2020-07-23 |
20200231967 | Double-stranded RNA Molecule Targeting CKIP-1 and Use Thereof - The present invention relates to the field of biomedicine, particularly to double-stranded RNA molecules targeting CKIP-1 and uses thereof, particularly to use of the double-stranded RNA molecules for the treatment of inflammatory diseases such as arthritis, particularly rheumatoid arthritis. | 2020-07-23 |
20200231968 | COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF FACTOR V - The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of Factor V, comprising an antisense strand having a nucleotide sequence which is less that 25 nucleotides in length and which is substantially complementary to at least a part of Factor V. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by the expression of Factor V using the pharmaceutical composition; and methods for inhibiting the expression of Factor V in a cell. | 2020-07-23 |
20200231969 | REDUCING INTRON RETENTION - Disclosed herein are methods, compositions, polynucleic acid polymers, assays, and kits for inducing processing of a partially processed mRNA transcript to remove a retained intron to produce a fully processed mRNA transcript that encodes a full-length functional form of a protein. Also described herein are methods and compositions for treating a disease or condition characterized by impaired production of a full-length functional form of a protein or for treating a disease or condition characterized by a defective splicing in a subject. | 2020-07-23 |
20200231970 | GENE THERAPIES FOR LYSOSOMAL DISORDERS - The disclosure relates, in some aspects, to compositions and methods for treatment of diseases associated with aberrant lysosomal function, for example Parkinson's disease and Gaucher disease. In some embodiments, the disclosure provides expression constructs comprising a transgene encoding one or more inhibitory nucleic acids targeting SCNA or a portion thereof, TMEM106B or a portion thereof, or any combination of the foregoing. In some embodiments, the disclosure provides methods of Parkinson's disease by administering such expression constructs to a subject in need thereof. | 2020-07-23 |
20200231971 | METHODS FOR TREATMENT OF POLYCYSTIC KIDNEY DISEASE - Provided herein are methods for the treatment of polycystic kidney disease, including autosomal dominant polycystic kidney disease, using modified oligonucleotides targeted to miR-17. | 2020-07-23 |
20200231972 | NUCLEIC ACID SIMULTANEOUSLY INHIBITING EXPRESSION OF AR GENE AND MTOR GENE - The present invention relates to nucleic acid molecules that simultaneously inhibit the expression of AR gene and mTOR gene, wherein the double-stranded siRNA and shRNA of the present invention were designed to simultaneously inhibit the expression of the AR gene and the mTOR gene which are associated with cancer. The double-stranded siRNA and shRNA of the present invention promote cancer cell death and synergistically enhance cancer cell death in combination with an anticancer agent, so that various types of cancer may be effectively prevented and treated. | 2020-07-23 |
20200231973 | ANTICANCER THERAPEUTIC INTERVENTION - The present invention is directed to a method of treating cancer using interfering RNA duplexes to mediate gene silencing. The present invention is also directed to interfering RNA duplexes and vectors encoding such interfering RNA duplexes. | 2020-07-23 |
20200231974 | A TWO-COMPONENT VECTOR LIBRARY SYSTEM FOR RAPID ASSEMBLY AND DIVERSIFICATION OF FULL-LENGTH T-CELL RECEPTOR OPEN READING FRAMES - A combined system comprising two separate components, wherein a first component is a vector carrying variable and constant (V-C) T-cell receptor (TCR) gene segments, and a second component is a vector carrying joining (J) TCR gene segments. The combined system may be modified so that said first component is a modified V-C entry vector encoding a first TCR chain, the system further comprises a fourth and a fifth component, wherein the fourth component comprises a Bidirectional Terminator (BiT) donor vector, and the fifth component comprises a modified V-C entry vector encoding a second TCR chain complimentary to said first TCR chain. | 2020-07-23 |
20200231975 | NOVEL TYPE VI CRISPR ORTHOLOGS AND SYSTEMS - The present disclosure provides for systems, methods, and compositions for targeting nucleic acids. In particular, the disclosure provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. | 2020-07-23 |
20200231976 | ITERATIVE NUCLEIC ACID ASSEMBLY USING ACTIVATION OF VECTOR-ENCODED TRAITS - Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits. | 2020-07-23 |
20200231977 | METHOD FOR IMPROVING TRANSFORMATION EFFICIENCY OF PLANT AND METHOD FOR TRANSFORMING PLANT - The present invention provides a method for improving transformation efficiency of a plant. The method according to the present invention comprises the use of a nucleic acid encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4, or a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 2 or 4, and having a function of improving transformation efficiency of a plant. | 2020-07-23 |
20200231978 | TARGETING OF GLUTEN BY GENOME EDITING - The present invention relates to methods for producing wheat lines that are low gliadin, low-gluten and transgene-free using genome editing. Also described are nucleic acid constructs and sgRNA molecules for use in genome editing, as well as genetically altered plants obtained by these methods. | 2020-07-23 |
20200231979 | TARGETED CHROMOSOMAL MUTAGENESIS USING ZINC FINGER NUCLEASES - The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time. | 2020-07-23 |
20200231980 | TARGETED MUTAGENESIS OF TOBACCO BERBERINE BRIDGE ENZYME-LIKE NUCLEIC ACIDS - The present invention relates to modifications of berberine bridge enzyme-like nucleic acids and their use in modulation of nicotine biosynthesis in plants. | 2020-07-23 |
20200231981 | QUALITY PROTEIN MAIZE BASED ON INCREASED SULFUR REDUCTION IN LEAF CELLS - Compositions and methods to produce maize with improved nutritional content are disclosed. | 2020-07-23 |
20200231982 | GENETIC LOCI ASSOCIATED WITH RESPONSE TO ABIOTIC STRESS - Compositions and methods useful in identifying and counter-selecting maize plants with having enhanced yield-related traits relative to control plants under abiotic stress conditions such as salt stress and/or drought are provided herein. The methods use molecular genetic markers to identify, select and/or construct salt stress tolerant and/or drought tolerant maize plants. Also provided are methods to enhance tolerance to salt stress and/or drought in crop plants by transforming crop plants with the | 2020-07-23 |
20200231983 | Plants Having Increased Tolerance to Herbicides - The present invention refers to a plant or plant part comprising a polynucleotide encoding a wildtype or mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such as azines. | 2020-07-23 |
20200231984 | INCREASED FUNGAL RESISTANCE IN CROP PLANTS - The present invention relates to methods for producing plants with increased fungal resistance, preferably seedling resistance against Northern Corn Leaf Blight. Further provided are methods for introducing, modifying, or modulating at least one wall-associated kinase (WAK) in(to) a plant cell, tissue, organ, or whole plant and thereby causing a reduced synthesis of benzoxazinoid and in turn increased fungal resistance. There are further provided methods to identify and/or modify downstream effector molecules in a WAK signalling cascade. Finally, plant cells, tissues, organs or whole plants having increased fungal resistance and methods using substances to activate signalling pathways in a targeted way are provided. The present invention thus relates to WAKs as master regulators and crucial signaling mediators in plant defense against fungal disease and the regulation and cross-talk mechanisms in the WAK signaling cascade and further gives examples for establishing novel anti-fungal strategies relevant for a series of crop plants. | 2020-07-23 |
20200231985 | COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS - Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as Coleopteran, Hemiptera, or Lepidopteran plant pest, including a | 2020-07-23 |
20200231986 | PRODUCTION OF ADENO-ASSOCIATED VIRUSES IN INSECT CELLS - This disclosure relates to the field of high scale production of recombinant Adeno-Associated Viruses (AAVs). The inventors have conceived of specific nucleic acid constructs that allow for high scale production of recombinant AAV particles in insect cells. Importantly, these nucleic constructs do not require the production of a heterologous AAP. This disclosure thus relates to a nucleic acid for producing AAV capsids in insect cells, where the nucleic acid includes a first open reading frame encoding the VP1, VP2, and VP3 proteins, and a second open reading frame encoding the Assembly-Activating Protein (AAP). | 2020-07-23 |
20200231987 | NUCLEIC ACID-GUIDED NUCLEASES - Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods. | 2020-07-23 |
20200231988 | ENGINEERED CLEAVAGE HALF-DOMAINS - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence. | 2020-07-23 |
20200231989 | METHODS AND COMPOSITIONS FOR THE TREATMENT OF LYSOSOMAL STORAGE DISEASES - Nucleases and methods of using these nucleases for inserting a sequence encoding a therapeutic protein such as an enzyme into a cell, thereby providing proteins or cell therapeutics for treatment and/or prevention of a lysosomal storage disease. | 2020-07-23 |
20200231990 | Yeast Cells and Methods for Producing Fatty Alcohols - The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of | 2020-07-23 |
20200231991 | LIMITING YEAST-PRODUCED TREHALOSE IN FERMENTATION - The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol). | 2020-07-23 |
20200231992 | Microbial Production of 2-Phenylethanol from Renewable Substrates - Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid. | 2020-07-23 |
20200231993 | BIOSYNTHESIS OF RETINOIDS - The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform. | 2020-07-23 |
20200231994 | BIOTECHNOLOGICAL PRODUCTION OF PROPANOL AND/OR PROPIONIC ACID - The present invention relates to a method of producing propanol and/or propionic acid, the method comprising:
| 2020-07-23 |
20200231995 | METHOD FOR PRODUCING ORGANIC ACID SALTS FROM FERMENTATION BROTH - The invention relates to a method for producing salts of butyric acid, lactic acid, propionic acid, valeric acid, acetic acid, glycolic acid, sorbic acid, fumaric acid, formic acid, malic acid, tartaric acid, citric acid, derivatives of said organic acids and mixtures thereof, comprising at least the successive steps of: ⋅i) culturing a microorganism in an appropriate culture medium comprising a carbon source and a nitrogen source, during a sufficient time to obtain a fermentation broth comprising the organic acid(s); ⋅ii) pre-treating said fermentation broth comprising at least clarification of the fermentation broth and separation of organic acids by evaporation of the clarified fermentation broth and then condensation of the volatile fraction containing organic acids (CVAF); ⋅v) adding inorganic C bases to said CVAF⋅vii) eliminating the remaining water of the CVAF, and recovering the organic acid salts. | 2020-07-23 |
20200231996 | PRODUCTION OF ALGAE-DERIVED POLYUNSATURATED FATTY ACIDS - Disclosed is a method for producing algae-derived polyunsaturated fatty acids, comprising the steps of a) contacting dry algae with ethanol; b) removing ethanol to obtain crude oil; c) subjecting the crude oil to a transesterification reaction with a lower alcohol to generate fatty acid alkyl esters; d) extracting the fatty acid alkyl esters to result in a phase containing fatty acid alkyl esters; e) collecting the phase containing fatty acid alkyl esters; and f) contacting the collected phase with silica gel. Also disclosed is a method for effectively removing impurities, including chlorophyll, fucoxanthin and beta-carotene, from crude algal fatty acid alkyl esters, comprising contacting the crude algal fatty acid alkyl esters with silica gel in hexane. | 2020-07-23 |
20200231997 | METHODS AND PRODUCTS FOR PRODUCTION OF WAX ESTERS - The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate. | 2020-07-23 |
20200231998 | HETEROLOGOUS PRODUCTION OF 10-METHYLSTEARIC ACID BY CELLS EXPRESSING RECOMBINANT METHYLTRANSFERASE - Disclosed herein are cells, nucleic acids, and proteins that can be used to produce branched (methyl)lipids, such as 10-methylstearic acids, and compositions that include such lipids. Cells disclosed herein comprise methyltransferase and/or reductase genes from bacteria of the class Gammaproteobacteria, which encode enzymes capable of catalyzing the production of branched (methyl)lipids from unbranched, unsaturated lipids. Saturated branched (methyl)lipids produced using embodiments of the present invention have favorable low-temperature fluidity and favorable oxidative stability, which are desirable properties for lubricants and specialty fluids. | 2020-07-23 |
20200231999 | MODIFIED LYSINE DECARBOXYLASE ENZYMES - The invention provides CadA polypeptides with mutations that increase activity in alkaline pH compared to the wild-type lysine decarboxylase. The invention also provides methods of generating such mutant polypeptides, microorganisms genetically modified to overexpress the mutant polypeptides, and methods of generating such microorganism. | 2020-07-23 |
20200232000 | BIOPRODUCTION OF PHENETHYL ALCOHOL, ALDEHYDE, ACID, AMINE, AND RELATED COMPOUNDS - This invention relates to the bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by subjecting a starting material comprising glucose, L-phenylalanine, substituted L-phenylalanine, styrene or substituted styrene to a plurality of enzyme catalyzed chemical transformations in a one-pot reaction system, using recombinant microbial cells overexpressing the enzymes. To produce phenylacetaldehyde from styrene, the cells are modified to overexpress styrene monooxygenase (SMO) and styrene oxide isomerase (SOI). To produce phenylacetic acid from styrene, SMO, SOI and aldehyde dehydrogenase are overexpressed. Alternatively, to produce 2-phenylethanol, SMO, SOI and aldehyde reductase or alcohol dehydrogenase are overexpressed, while to produce phenylethylamine, SMO, SOI and transaminase are overexpressed. | 2020-07-23 |
20200232001 | MICROBIAL PRODUCTION OF ALKANOLAMIDES AND AMIDOAMINES AND USES THEREOF - The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source. | 2020-07-23 |
20200232002 | METHOD FOR ENZYMATIC PREPARATION OF R-3 AMINOBUTYRIC ACID - An R-3-aminobutyric acid preparation method with high efficiency and high stereoselectivity. The method comprises using aspartase with stereoisomerization catalytic activity derived from | 2020-07-23 |
20200232003 | SUBTERRANEAN MICROALGAE FOR PRODUCTION OF MICROBIAL BIOMASS, SUBSTANCES, AND COMPOSITIONS - The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus | 2020-07-23 |
20200232004 | Glycosylated Beta-Galactosidase Compositions Having improved Transgalactosylating Activity - The present invention relates to compositions, particularly liquid compositions, comprising polypeptides having beta-galactosidase activity, methods of making said compositions, and uses of the compositions for making e.g. dairy products. The polypeptides having beta-galactosidase activity are modified by glycation of lysine and/or arginine residues by incubating the enzyme in the presence of reducing sugars, optionally combined with a heat treatment. Thereby, transgalactosylating activity is increased. | 2020-07-23 |
20200232005 | Methods of Producing and Using Single-Stranded Deoxyribonucleic Acids and Compositions for Use in Practicing the Same - Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention. | 2020-07-23 |
20200232006 | DEVICE AND METHOD FOR THE MICROBIOLOGICAL EXAMINATION OF WASHING MACHINES - The invention relates to a bioindicator and a method for using the bioindicator for microbiological testing of washing machines. The device according to the invention allows a safe, reliable, cost-effective and easy-to-use microbiological test of the disinfection performance of washing machines. The invention further relates to a use of the bioindicator and a kit comprising 10 bioindicators. In particular, the invention relates to a closed bioindicator for a verification of disinfecting washing processes. | 2020-07-23 |
20200232007 | SCREENING FOR L-FORM BACTERIA - Disclosed herein are methods for screening clinical or biological samples to determine the presence of L-form bacteria within the sample. Methods include contacting a sample to a liquid growth medium and incubating the liquid growth medium at a temperature lower than 37 degrees C. The liquid growth medium is monitored for L-form bacterial growth. An amount of the liquid growth medium is transferred as an inoculant to a solid growth medium, and the solid growth medium is incubated under conditions that maintain a hydrated state of the inoculant to enable the L-form bacteria to efficiently interface with the solid growth medium and continue to grow. | 2020-07-23 |
20200232008 | METHOD FOR INLINE BILAYER CAPACITANCE MONITORING - A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage. | 2020-07-23 |
20200232009 | Screening of Nucleic Acid Sequence Variations via Random Loading of Optically-Encoded Particles - Embodiments disclosed herein provide reagents and methods for high-throughput screening of nucleic acid sequence variations in nucleic acid containing specimens. Nucleic acid specimens to be screened are loaded into separate discrete volumes. Optically encoded particles are used to deliver primers to amplify one or more sequences comprising the nucleic acid sequence variation. The optically encoded particles may be delivered to the discrete volumes culture randomly resulting in a random combination of optically encoded particles in each well, or a unique combination of optically encoded particles may be specifically assigned to each discrete volume. The observable combination of optically encoded particles may then be used to identify each discrete volume. | 2020-07-23 |
20200232010 | METHODS, COMPOSITIONS, AND SYSTEMS FOR IMPROVING RECOVERY OF NUCLEIC ACID MOLECULES - In an aspect, the present disclosure provides a method for analyzing nucleic acid molecules in a sample of polynucleotides, comprising: (a) adding a subset of fragment size control molecules to the nucleic acid molecules, thereby producing a first spike-in sample; (b) extracting nucleic acids from the first spike-in sample; (c) processing at least a subset of the extracted nucleic acids, thereby producing a processed sample, wherein the processing comprises partitioning, tagging, and/or amplifying at least a subset of the first spike-in sample; (d) enriching for at least a subset of the processed sample, thereby producing an enriched sample; (e) sequencing at least a subset of the enriched sample to generate sequence reads; (0 analyzing the sequence reads to generate fragment size scores of the subset of fragment size control molecules; and (g) comparing the fragment size scores with fragment size thresholds. | 2020-07-23 |
20200232011 | METHODS OF NUCLEIC ACID DETECTION AND PRIMER DESIGN - Provided herein are methods for detection of a target nucleic acid from a single cell. Preferred embodiments of the method include selecting one or more target nucleic acid sequence of interest in an individual cell, where the target nucleic acid sequence is typically complementary to cellular DNA, including a genomic DNA, and an RNA in a cell. A cell sample is provided, and in preferred embodiments the sample is from a single cell. The cell is lysed and in a single reaction both DNA and RNA can be detected without sub-dividing the sample. This can be accomplished by providing nucleic acid amplification primer sets complementary to one or more target nucleic acid, and in particular primer sets that selectively amplify particular target nucleic acids or amplicons in an amplification reaction. Also provided are methods of primer design for these methods and apparatus and system used to perform the methods. | 2020-07-23 |
20200232012 | NUCLEIC ACID COMPLEX PAIR, COMPETITIVE CONSTRUCT, AND PCR KIT USING THE SAME - According to an exemplary embodiment of the present application, provided is a nucleic acid complex pair, which includes a first nucleic acid complex including a first determinant and a second tag; and a second nucleic acid complex including a second determinant and a second tag, wherein the first determinant includes a forward primer for first target DNA, the second determinant includes a reverse primer for the first target DNA, the first tag includes a base sequence complementary to the base sequence of the second tag, and the second tag includes a base sequence complementary to the base sequence of the first tag. | 2020-07-23 |
20200232013 | HEATING MECHANISM FOR DNA AMPLIFICATION, EXTRACTION OR STERILIZATION USING PHOTO-THERMAL NANOPARTICLES - A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented. | 2020-07-23 |
20200232014 | SYSTEMS AND METHODS FOR ISOLATING NUCLEIC ACIDS - The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating low molecular weight circulating nucleic acids from bodily fluids (e.g., plasma). | 2020-07-23 |
20200232015 | METHOD FOR DETECTING BRUCELLA INFECTION AND APPLICATION THEREOF - The present invention provides a method for detecting | 2020-07-23 |
20200232016 | Methods and Compositions for the Analysis of Biological Molecules - Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analyses on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices. | 2020-07-23 |
20200232017 | HIGH INTENSITY LABELED REACTANT COMPOSITIONS AND METHODS FOR SEQUENCING - Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided. | 2020-07-23 |
20200232018 | HYBRIDIZATION COMPOSITIONS AND METHODS USING FORMAMIDE - The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%. | 2020-07-23 |
20200232019 | IMPROVEMENTS IN OR RELATING TO CELL ANALYSIS - The present invention provides a method for cell analysis, comprising: preparing a blood sample comprising nucleated cells having surface, cytoplasmic or nuclear antigens (markers); antibody staining the cell markers; fixing and permeabilising the cells; FISH probe hybridising to chromosomes in the cells; performing imaging flow cytometry on the cells; analysing data obtained from performing imaging flow cytometry; and diagnosing, prognosing or monitoring a medical condition based on the data analysis. | 2020-07-23 |
20200232020 | Partition-free digital PCR (dPCR) system - The present disclosure relates to methods and systems for partition-free quantification of molecules. The methods and systems provided allow a sample to be amplified such that discrete amplification spots can be analyzed to quantify the number of molecules without requiring physical partitions in order to separate the amplification spots. | 2020-07-23 |
20200232021 | Method for Detecting Tumor DNA in a cfDNA Sample Collected from a Patient that has Previously Undergone Cancer Therapy - The present invention provides a method for detecting a genetic variant in a region of interest in a DNA sample comprising (i) determining, for a given sequencing platform, sequencing process and sequencing depth, the distribution of the number of reads supporting a genetic variant or plurality of genetic variants expected to be observed in the sequencing results of amplification reactions due to amplification and sequencing error (read count distribution); (ii) based on the read count distribution determined in step (i), establishing a threshold frequency at or above which the genetic variant must be observed in sequencing results of amplification reactions to assign a positive determination for the presence of the genetic variant in a given amplification reaction; (iii) partitioning the DNA sample into a plurality of replicate amplification reactions, so that the mean number of amplifiable template molecules of the region of interest in a replicate amplification reaction is fewer than the reciprocal of the threshold frequency determined in step (ii); (iv) performing the amplification reactions of step (iii) and sequencing the products of amplification reactions, (v) based on step (ii) and the results of step (iv), determining the presence/absence of the genetic variant in each replicate amplification reaction; and (vi) integrating the results of (v) to determine the presence/absence of the genetic variant in the region of interest in the DNA sample. | 2020-07-23 |
20200232022 | METHODS OF ASSESSING NUCLEASE CLEAVAGE - The present disclosure relates to methods of analyzing activity of nucleases, such as ZFNs, TALENs or CRISPR-associated nucleases, in particular their cutting at “off-target” loci. | 2020-07-23 |
20200232023 | CYANINE DYES - The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates. | 2020-07-23 |
20200232024 | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING - Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule. | 2020-07-23 |
20200232025 | DNA SEQUENCING WITH STACKED NANOPORES - A method for fabricating a stacked nanopore includes forming a stack of layers having alternating conductive lines and dielectric layers on a substrate, and patterning the stack to form a staircase structure with the conductive lines having a length gradually changing at each level in the stack. The method also includes depositing and planarizing a dielectric material over the staircase structure, forming contacts through the dielectric material to the conductive lines for each level of conductive lines, etching a nanopore through the stack of layers to form pairs of opposing electrodes across the nanopore using the conductive lines; and opening up the substrate to expose the nanopore. | 2020-07-23 |
20200232026 | BASECALLING FOR STOCHASTIC SEQUENCING PROCESSES - Techniques for measuring sequences of nucleic acids are provided. Time-based measurements (e.g., forming a histogram) particular to a given sequencing cell can be used to generate a tailored model. The model can include probability functions, each corresponding to different states (e.g., different states of a nanopore). Such probability functions can be fit to a histogram of measurements obtained for that cell. The probability functions can be updated over a sequencing run of the nucleic acid so that drifts in physical properties of the sequencing cell can be compensated. A hidden Markov model can use such probability functions as emission probabilities for determining the most likely nucleotide states over time. For sequencing cells involving a polymerase, a 2-state classification between bound and unbound states of the polymerase can be performed. The bound regions can be further analyzed by a second classifier to distinguish between states corresponding to different bound nucleotides. | 2020-07-23 |
20200232027 | Methods and Systems for Processing Polynucleotides - The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides. | 2020-07-23 |
20200232028 | SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS - Provided herein are devices and methods suitable for sequencing, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other biomolecules. | 2020-07-23 |
20200232029 | SYSTEMS AND METHODS FOR MITOCHONDRIAL ANALYSIS - The invention provides methods of analyzing an individual's mtDNA by transforming available reference sequences into a directed graph that compactly represents all the information without duplication and comparing sequence reads from the mtDNA to the graph to identify the individual or describe their mtDNA. A directed graph can represent all of the genetic variation found among the mitochondrial genomes across all of a number of reference organisms while providing a single article to which sequence reads can be aligned or compared. Thus any sequence read or other sequence fragment can be compared, in a single operation, to the article that represents all of the reference mitochondrial sequences. | 2020-07-23 |
20200232030 | MASSIVELY MULTIPLEXED RNA SEQUENCING - The disclosed invention is related to a universal strand-specific protocol for the sequencing preparation of all classes of RNA. The protocol allows for sequencing for dozens to more than thousands of samples simultaneously. Specifically, the disclosed invention is a method for parallel sequencing target RNA from samples from multiple sources while maintaining source identification. The method includes providing samples of RNA comprising target RNA from two or more sources; labeling, at the 3′ end, the RNA from the two or more sources with a first nucleic acid adaptor that comprises a nucleic acid sequence that differentiates between the RNA from the two or more sources; reverse transcribing the two or more sources to create a single stranded DNA comprising the nucleic acid sequence that differentiates between the RNA from the two or more sources; amplifying the single stranded DNA to create DNA amplification products that comprise the nucleic acid sequence that differentiates between the RNA from the two or more sources; sequencing the DNA amplification products thereby parallel sequencing target RNA from samples from multiple sources while maintaining source identification. | 2020-07-23 |
20200232031 | METHOD OF DIAGNOSING AND TREATING ACUTE REJECTION IN KIDNEY TRANSPLANT PATIENTS - Provided are a method of diagnosing the onset of acute rejection, the method including the step of correlating expression levels of one or more miRNAs selected from the group consisting of 8 kinds of miRNAs including miR-4488, miR-4532, miR-146a-5p, miR-21-5p, miR-373-3p, miR-31-5p, miR-223-3p, and miR-210-3p, and a combination thereof with the onset of acute rejection in kidney transplant patients, a composition for diagnosing acute rejection, the composition including an agent capable of measuring the expression level of each of the miRNAs, a kit for diagnosing acute rejection, the kit including the composition, and a method of treating acute rejection, the method including the step of treating acute rejection, when the onset of acute rejection is identified by the above diagnostic method. Since miR-4488, miR-4532, miR-146a-5p, miR-21-5p, miR-373-3p, miR-31-5p, miR-223-3p, and miR-210-3p according to the present invention exist in high levels in the urine of a patient with acute kidney transplant rejection, they may be used to easily diagnose the onset of acute kidney transplant rejection, thereby being used in the effective treatment of acute rejection after kidney transplantation. | 2020-07-23 |
20200232032 | OLIGONUCLEOTIDES ASSOCIATED WITH ANTIBODIES - Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12). | 2020-07-23 |
20200232033 | PLATFORM INDEPENDENT HAPLOTYPE IDENTIFICATION AND USE IN ULTRASENSITIVE DNA DETECTION - The present invention provides methods for analyzing blocks of closely spaced SNPs, or haplotypes for use in identification of the origin of DNA in a sample. The methods comprise aligning common alleles of a gene of interest and identifying a region containing a plurality of SNPs which is flanked by non-polymorphic DNA which can be used for primer placement. Any sequencing method, including next generation sequencing methods can then be used to determine the haplotypes in the sample with a lower limit of detection of at least 0.01%. These inventive methods are useful, for example, for identification of hematopoietic stem cell transplantation patients destined to relapse, microchimerism associated with solid organ transplantation, detection of solid organ transplant rejection by detecting donor DNA in recipient plasma, forensic applications, and patient identification. | 2020-07-23 |
20200232034 | METHODS OF DETECTING OSTEOARTHRITIS AND PREDICTING PROGRESSION THEREOF - Provided herein are methods and biomarkers useful for detecting and diagnosing osteoarthritis and predicting the progression of osteoarthritis in subjects. The diagnoses and predictions of prognosis may be used to develop treatment plans for subjects. Also included are methods of treating subjects and administering pharmaceuticals based on the diagnosis and prognosis predictions. | 2020-07-23 |
20200232035 | GENETIC TESTING FOR INHERITED PERIPHERAL NEUROPATHY - Methods for diagnosing propensity to exhibit acquired peripheral neuropathy in dogs are described. The methods and kits test dogs for presence of a disease-associated genomic variant. Presence of the genomic variant indicates an increased likelihood of the dog developing an acquired peripheral neuropathy. This information can be used to guide preemptive clinical treatment of the animal for peripheral neuropathy and to choose dogs for selective breeding programs. | 2020-07-23 |
20200232036 | SYSTEM AND METHOD FOR CLEANING NOISY GENETIC DATA AND DETERMINING CHROMOSOME COPY NUMBER - Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents. | 2020-07-23 |
20200232037 | METHODS FOR SIMULTANEOUS AMPLIFICATION OF TARGET LOCI - The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons. | 2020-07-23 |
20200232038 | METHODS OF DETECTING CANCER RECURRENCE - Disclosed are methods of detecting the likelihood of cancer recurrence. More particularly, the present invention discloses methods of identifying nucleic acid signatures that correlate with the likelihood of cancer recurrence, and methods of using such signatures. | 2020-07-23 |
20200232039 | ADM2 GENE MARKER FOR DIAGNOSIS OR PROGNOSIS PREDICTION OF THYROID CANCER AND USES THEREOF - The present invention relates to a composition for diagnosis or prognosis prediction of thyroid cancer, which includes an agent for measuring the expression level of mRNA of ADM2 gene or a protein thereof, a kit for diagnosis or prognosis prediction of thyroid cancer, which includes the composition, and a method for providing information for diagnosis or prognosis prediction of thyroid cancer using the composition or the kit. | 2020-07-23 |
20200232040 | NEOANTIGENS AND USES THEREOF FOR TREATING CANCER - Systems and methods for determining the likely responsiveness of a human cancer subject to a checkpoint blockade immunotherapy regimen are provided. Sequencing reads are obtained from samples from the subject representative of the cancer. A human leukocyte antigen type and a plurality of clones is determined from the sequencing reads. For each clone, an initial frequency X | 2020-07-23 |
20200232041 | Methods For Diagnosis And Treatment Of Patients Having Solid Tumors - Methods for diagnosing a subject as a candidate for removal of a solid tumor without preoperative chemoradiation therapy, and methods for treating patients having solid tumors, who have one or more of genomic instability, elevated double stranded DNA breaks, elevated gamma-H2AX foci, or elevated replication stress and/or double stranded break-signalling (DSB-signalling) biomarkers in peripheral blood lymphocytes (PBLs) are provided herein. | 2020-07-23 |
20200232042 | METHODS FOR DETERMINING RESPONSE TO PARP INHIBITORS - Methods and compositions are provided for determining whether an adenocarcinoma or squamous cell carcinoma patient is likely to respond to PARP inhibitor treatment. Specifically, a method of assessing whether a patient's adenocarcinoma (AD) lung cancer subtype is terminal respiratory unit (TRU), proximal inflammatory (PI), or proximal proliferative (PP) or a patient's squamous cell carcinoma (SQ) is primitive, classical, secretory or basal is provided herein. The method entails detecting the levels of classifier biomarkers at the nucleic acid level, in an AD or SQ lung cancer sample obtained from the patient. Based in part on the levels of the classifier biomarkers, the AD lung cancer sample is classified as a TRU, PI, or PP AD sample or the SQ lung cancer sample is classified as primitive, classical, secretory or basal and a determination of whether the patient is likely to respond to PARP inhibitor treatment can be made. | 2020-07-23 |
20200232043 | METHOD FOR PREDICTING THE RESPONSE OF NON-SMALL-CELL LUNG CARCINOMA PATIENTS TO A MEDICAMENT - The present invention relates to a method for determining or predicting the response of a patient diagnosed with non-small-cell lung carcinoma to targeted pharmacotherapy. The present invention also aims to provide methods and devices for predicting the response of patients diagnosed with non-small-cell lung carcinoma to specific medicaments. More specifically, the present invention provides methods which measure kinase-activity by studying phosphorylation levels and profiles and inhibitions thereof by drugs in blood samples of said patients. | 2020-07-23 |
20200232044 | TUMOR AND METASTASIZING MARKER - The present invention relates to methods for detecting a malignant tumor in an individual by means of determining the Vimentin variant 3 (Vim3) level in the individual's blood serum S and/or the accumulation of Vim3 polypeptide in cell nuclei. Furthermore, the present invention refers to an antineoplastic agent for use in a method for treating an individual bearing a malignant tumor, wherein said antineoplastic agent is a Vim3 inhibitor and/or wherein the malignant tumor is endothelin B receptor negative. | 2020-07-23 |
20200232045 | METHODS OF IDENTIFYING INDIVIDUALS AT INCREASED RISK OF LUNG CANCER - The invention provides the identification of oncogenic pathways activated in cytologically normal airway cells of individuals having or at risk of having lung disease, as well as specific gene expression patterns (biomarkers) associated with pathway activation. These biomarkers and pathways may provide prognostic and/or diagnostic indicators in lung disease, e.g., lung cancer. Additionally, these pathways and biomarkers may provide therapeutic targets for the treatment of lung disease, as well as markers for the assessment of treatment efficacy. | 2020-07-23 |
20200232046 | GENOMIC SEQUENCING CLASSIFIER - Provided herein are methods and systems for analyzing a sample of a subject by using a trained algorithm to classify the samples as benign, suspicious for malignancy, or malignant. Further disclosed herein are methods and systems for identifying genetic aberrations to indicate risk of malignancy. | 2020-07-23 |
20200232047 | MOLECULAR ASSAY METHODS - Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. | 2020-07-23 |
20200232048 | MOLECULAR MARKERS ASSOCIATED WITH SOY IRON DEFICIENCY CHLOROSIS - The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having iron deficiency chlorosis tolerance. A soybean plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a soybean plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided. | 2020-07-23 |
20200232049 | METHOD FOR DETERMINING DESIRED CELL TYPE USING EXPRESSION OF miRNA AS INDICATOR - A method is provided for distinguishing living cells in a living state with high accuracy. Provided is a method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator. | 2020-07-23 |