30th week of 2021 patent applcation highlights part 29 |
Patent application number | Title | Published |
20210230553 | METHOD FOR EVALUATING QUALITY OF HUMAN MESENCHYMAL STEM CELL, AND MONOCLONAL ANTIBODY FOR USE IN SAID METHOD - The present invention aims to obtain a method for quality evaluation of human mesenchymal stem cells, a method for isolation, selection and culture of human mesenchymal stem cells, a cell population of rapidly proliferating human mesenchymal stem cells, as well as monoclonal antibodies that specifically recognize rapidly proliferating human mesenchymal stem cells. | 2021-07-29 |
20210230554 | GENERATION OF FUNCTIONAL BETA CELLS FROM HUMAN PLURIPOTENT STEM CELL-DERIVED ENDOCRINE PROGENITORS - The present invention relates to method of generation of functional mature beta cells from human pluripotent stem cell-derived endocrine progenitors. The present invention also relates to functional mature beta cells produced by said methods and uses of said mature beta cells for treating diabetes. | 2021-07-29 |
20210230555 | OVARIAN CANCER ORGANOID CULTURE - The present invention relates to a method for the production of a culture, e.g. an organoid culture of ovarian cancer or cancer precursor cells, particularly of high grade serous ovarian carcinoma cells. By means of this method, an organoid culture of ovarian cancer or cancer precursor cells and a biobank comprising a plurality of different organoid cultures of ovarian cancer or cancer precursor cells may be generated. Further, a culture medium suitable for the long-term culture of ovarian cancer or cancer precursor cells is provided. Furthermore, use of the organoid culture and the biobank for medical applications, e.g. in the field of diagnostics, and therapy and in the field of drug screening is described. | 2021-07-29 |
20210230556 | IN VITRO PRODUCTION OF EXPANDED POTENTIAL STEM CELLS - A culture medium is provided which is capable of establishing expanded potential stem cell (EPSC) lines which resemble naive or ground state ES cells, but are also able to differentiate into placenta trophoblasts and the embryo proper. Methods are provided using the medium for the in vitro conversion and maintenance of cells, including pluripotent cells into EPSCs. | 2021-07-29 |
20210230557 | GENERATION OF INDUCED PLURIPOTENT STEM CELLS (IPSCs) WITH GLUT2 MUTATION OR FOXA2 DELETION, METHODS OF PREPARING SAME, AND METHODS OF USING SAME - The present disclosure generally relates to the method of generating human induced pluripotent stem cells (hiPSC) from patients with either heterozygous deletion of the FOX2A gene or GLUT2 (SLC2A2) gene mutations. The methods disclosed herein generate human cell models for diabetes, Fanconi Bickel Syndrome (FBS), dysmporphic features, growth hormone deficiency, central hypothyroidism, and related disorders. The methods disclosed herein generate patient-specific iPSC cell lines that could be used by researchers to perform mechanistic studies, drug screening, and understanding the role of GLUT2 or FOXA2 in specific human cell types. | 2021-07-29 |
20210230558 | Dispersion of Pluripotent Stem Cells, and Pluripotent Stem Cell Product and Method for Producing Same - An object of the present invention is to provide a dispersion of pluripotent stem cells and a pluripotent stem cell product having a suitable quality as a pharmaceutical or a raw material for producing a pharmaceutical which can be stored and distributed, and a method for producing the same. The method for producing a pluripotent stem cell product of the present invention comprises (1) culturing pluripotent stem cells in an undifferentiation maintenance medium; (2) suspending the pluripotent stem cells cultured in step (1) in a first medium for suspending cells containing a ROCK inhibitor; (3) replacing the medium for suspending cells of a suspension obtained in step (2) with a medium for cryopreservation to obtain a cell dispersion solution consisting of the medium for cryopreservation and the pluripotent stem cells dispersed in the medium for cryopreservation; and (4) filling the cell dispersion solution obtained in step (3) into an airtight container so as to be in an airtight state. | 2021-07-29 |
20210230559 | PROPAGATOR CELLS AND METHODS FOR PROPAGATING PHAGE, IN PARTICULAR FOR DELIVERING CRISPR-CAS COMPONENTS VIA PROBIOTIC ORGANISMS - The invention provides propagator cells and methods for propagating phage and transduction particles. | 2021-07-29 |
20210230560 | SYNTHETIC CHIMERIC VACCINIA VIRUS - The invention relates in various aspects to a synthetic chimeric vaccinia virus or compositions comprising such viruses, and the development and use of systems and methods for producing such synthetic chimeric vaccinia viruses. The synthetic chimeric vaccinia viruses are well suited, among others, as virus vaccines or to generate an oncolytic response and pharmaceutical formulations. | 2021-07-29 |
20210230561 | GLUCOSE OXIDASE GOD MUTANT AND GENE AND APPLICATION THEREOF - The present invention takes the glucose oxidase GOD from | 2021-07-29 |
20210230562 | OMEGA-HYDROXYLASE-RELATED FUSION POLYPEPTIDE VARIANTS WITH IMPROVED PROPERTIES - The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives. | 2021-07-29 |
20210230563 | MUTANT P-HYDROXYPHENYLPYRUVATE DIOXYGENASE, AND CODING NUCLEIC ACID AND USE THEREOF - The present invention relates to a mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof and an isolated polynucleotide comprising a nucleic acid sequence encoding the protein or fragment thereof, wherein the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof retains or enhances the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and is significantly less sensitive to HPPD-inhibiting herbicides than a wild-type HPPD. The present invention also relates to a nucleic acid construct, an expression vector and a host cell comprising the polynucleotide, as well as to a method for producing a plant that has the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and significantly reduced sensitivity to HPPD-inhibiting herbicides. | 2021-07-29 |
20210230564 | TRANSAMINASE POLYPEPTIDES - The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds. | 2021-07-29 |
20210230565 | BRUTON'S TYROSINE KINASE HOMING ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF USE - The present disclosure provides improved genome editing compositions and methods for editing a human BTK gene. The disclosure further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of X-linked agammaglobulinemia (XLA). | 2021-07-29 |
20210230566 | ACINETOBACTER AND USE THEREOF IN PRODUCTION OF CHIRAL 3-CYCLOHEXENE-1-CARBOXYLIC ACID - The invention discloses a strain of | 2021-07-29 |
20210230567 | MUTANT CPF1 ENDONUCLEASES - The present invention relates to mutant Cpf1 endonucleases having altered activity compared to the wild type Cpf1, and their use to introduce single strand breaks in nucleic acid sequences. Methods for detection and quantification of a nucleic acid sequence are also disclosed. Methods for diagnosis of an infectious disease are also disclosed. | 2021-07-29 |
20210230568 | MICROHOMOLOGY MEDIATED REPAIR OF MICRODUPLICATION GENE MUTATIONS - The present invention is directed to the filed of gene therapy. In particular, compositions and methods are disclosed that repair gene microduplication mutations by reversion to a wild type sequence. For example, the creation of a double stranded break by a programmable nuclease protein within a microduplication induces the microhomology mediated end joining DNA repair pathway that in the process of DNA repair removes the microduplication mutation and restores the wild type sequence. | 2021-07-29 |
20210230569 | TREATMENT OF GLUTEN INTOLERANCE AND RELATED CONDITIONS - Provided herein are compositions, foods comprising nepenthesin or a derivative thereof and methods of using nepenthesin or a derivative thereof for modulating gluten intolerance and related conditions, such as celiac disease. Further provided herein are pharmaceutical compositions comprising nepenthesin or a derivative thereof and methods of using nepenthesin or a derivative thereof to treat bacterial infections of the gastrointestinal tract, such as | 2021-07-29 |
20210230570 | MODIFIED FACTOR IX POLYPEPTIDES AND USES THEREOF - Modified Factor IX (FIX) polypeptides and uses thereof are provided. Nucleic acid molecules encoding modified factor IX (FIX) polypeptides and uses thereof also are provided. Such modified FIX polypeptides include FIXa and other forms of FIX. Among the modified FIX polypeptides provided are those that have altered activities, typically altered procoagulant activity, including increased procoagulant activities. Hence, such modified polypeptides are therapeutics. | 2021-07-29 |
20210230571 | VARIANTS OF CHYMOSIN WITH IMPROVED MILK-CLOTTING PROPERTIES - Variants of chymosin with improved milk-clotting properties are disclosed. | 2021-07-29 |
20210230572 | ACETOHYDROXY ACID SYNTHASE VARIANT, MICROORGANISM COMPRISING THE SAME, AND METHOD OF PRODUCING L-BRANCHED-CHAIN AMINO ACID USING THE SAME - The present disclosure relates to a novel acetohydroxy acid synthase, a microorganism comprising the same, or a method for producing an L-branched-chain amino acid using the same. | 2021-07-29 |
20210230573 | MICROORGANISMS AND THE PRODUCTION OF FINE CHEMICALS - Described herein is the fermentative production of fine chemicals, notably itaconate or itaconic acid, including production microorganisms, fermentation compositions and media, proteins useful in the production of the products, and nucleic acids for expression of such proteins, as well as methods for the production of fine chemicals. | 2021-07-29 |
20210230574 | ORGANIC-INORGANIC HYBRID NANOFLOWER AND PREPARATION METHOD THEREOF - The technical field of enzyme immobilization, and particularly, an organic-inorganic hybrid nanoflower and a preparation method thereof. The organic-inorganic hybrid nanoflower is a flower-like immobilized enzyme formed by self-assembly of a layered rare earth compound as an inorganic carrier and a biological enzyme as an organic component. The layered rare earth compound is Ln | 2021-07-29 |
20210230575 | USE OF NANOSECOND PULSED ELECTRIC FIELD IN IMPROVING CELL STEMNESS - Provided is the use of a nanosecond pulsed electric field in improving cell stemness. Specifically, applying a nanosecond pulsed electric field to cells, especially stem cells, can effectively increase the expression level of stemness genes and reduce the methylation level of the cells, improve the stemness of the stem cells, and further facilitate the ability of the stem cells to receive induced differentiation. | 2021-07-29 |
20210230576 | SYSTEMS, DEVICES, AND METHODS FOR ISOTACHOPHORESIS - The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing. | 2021-07-29 |
20210230577 | CYTOSINE TO GUANINE BASE EDITOR - Some aspects of this disclosure provide compositions, strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins capable of inducing a cytosine (C) to guanine (G) change in a nucleic acid (e.g., genomic DNA) are provided. In some embodiments, fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9) and nucleic acid editing proteins or protein domains, e.g., deaminase domains, polymerase domains, and/or base excision enzymes are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9), and nucleic acid editing proteins or domains, are provided. | 2021-07-29 |
20210230578 | REMOVAL OF DNA FRAGMENTS IN MRNA PRODUCTION PROCESS - The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures for obtaining an in vitro transcription product, and removing any DNA from the product. The DNA can be removed by adding either free DNase or a resin containing immobilized DNase to the product, and recovering the RNA transcript. Alternatively, the DNA template used in the in vitro transcription reaction is labeled. After transcription, the product is applied to a resin that is configured to bind the label, and the RNA transcript is recovered. To detect whether any residual impurities are left in the RNA transcript product, the product is subjected to nuclease digestion and subsequently to liquid chromatography-tandem mass spectrometry analysis to quantitate any residual DNA. The present invention demonstrates efficient and effective methods of isolating an RNA transcript from an in vitro transcription product. | 2021-07-29 |
20210230579 | TARGET MATERIAL EXTRACTION APPARATUS WITH REPLACEMENT OF MAGNETIC BAR BLOCK ALLOWED - The present invention relates to an extraction apparatus capable of simultaneously extracting target materials from multiple biological samples and, more particularly, to a target material extraction apparatus in which magnetic bar blocks can be replaced according to kinds of multi-well plates to be inserted into a cartridge. When used, the target material extraction apparatus of the present invention is operated in such a manner that among various multi-well plates having different numbers of wells, multi-well plates suitable for a use purpose are loaded into a cartridge within the apparatus and magnetic bar blocks equipped with magnetic bars suitable therefor are selected and loaded. Thus, the apparatus has the advantage of selectively applying various multi-well plates to one installment according to the number of samples from which a target material is extracted or to the amount of the target material to be extracted. | 2021-07-29 |
20210230580 | QUALITY CONTROL REAGENTS AND METHODS FOR SERUM ANTIBODY PROFILING - Disclosed herein are reagents for use in quality control assessment of antibody profiling platforms, such as biopanning and Digital Serology. Also disclosed herein are kits, method of manufacturing, and methods of using the same. | 2021-07-29 |
20210230581 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2021-07-29 |
20210230582 | COMPOSITIONS AND KITS FOR MOLECULAR COUNTING - Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins. | 2021-07-29 |
20210230583 | BARCODED WELLS FOR SPATIAL MAPPING OF SINGLE CELLS THROUGH SEQUENCING - Disclosed herein include systems, methods, compositions, and kits for methods of assigning sequencing data to partitions. There are provided, in some embodiments, methods of associating sequencing data and phenotypic data of single cells. There are provided, in some embodiments, methods of reducing noise in sequencing data. Disclosed herein include partition indexing oligonucleotides comprising a partition indexing sequence. The partition indexing oligonucleotides can be associated with partitions. Partition indexing oligonucleotides situated within the same partition can comprise the same partition indexing sequence. Partition indexing oligonucleotides situated within different partitions comprise different partition indexing sequences. | 2021-07-29 |
20210230584 | LIGATION MEDIATED ANALYSIS OF NUCLEIC ACIDS - The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell. | 2021-07-29 |
20210230585 | KIT, SYSTEM, AND FLOW CELL - An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid. | 2021-07-29 |
20210230586 | Expanding the Chemical Substrates for Genetic Code Reprogramming - Disclosed are methods, systems, components, and compositions for synthesis of sequence defined polymers. The methods, systems, components, and compositions may be utilized for incorporating novel substrates that include non-standard amino acid monomers and non-amino acid monomers into sequence defined polymers. As disclosed herein, the novel substrates may be utilized for acylation of tRNA via flexizyme catalyzed reactions. The tRNAs thus acylated with the novel substrates may be utilized in synthesis platforms for incorporating the novel substrates into a sequence defined polymer. | 2021-07-29 |
20210230587 | NOVEL METHOD FOR STABILIZING NUCLEIC ACID NANOSTRUCTURES - The present invention relates to a novel method for stabilizing nucleic acid nanostructures by curing with ultraviolet light, particularly by crosslinking pyrimidine nucleotides. | 2021-07-29 |
20210230588 | METHODS AND SYSTEMS OF MOLECULAR RECORDING BY CRISPR-CAS SYSTEM - This invention provides methods of altering a cell including providing the cell with a nucleic acid sequence encoding a Cas1 protein and/or a Cas2 protein of a CRISPR adaptation system, providing the cell with a CRISPR array nucleic acid sequence including a leader sequence and at least one repeat sequence, wherein the cell expresses the Cas1 protein and/or the Cas2 protein and wherein the CRISPR array nucleic acid sequence is within genomic DNA of the cell or on a plasmid. Also provided are methods and systems for nucleic acid storage and in vivo molecular recordings of events into a cell. | 2021-07-29 |
20210230589 | Compositions for Modulating C9ORF72 Expression - Disclosed herein are compositions and methods for reducing expression of C9ORF72 mRNA and protein in an animal. Such methods are useful to treat, prevent, ameliorate, or slow progression of neurodegenerative diseases in an individual in need thereof. | 2021-07-29 |
20210230590 | STEREOSPECIFIC LINKAGES IN RNA EDITING OLIGONUCLEOTIDES - The invention relates to editing oligonucleotides (EONs) that carry stereospecific phosphorothioate internucleotide linkage modifications at specified positions and that do not carry such modifications on positions that would lower RNA editing efficiency. The selection of positions that should or should not carry a phosphorothioate Rp and/or Sp configuration modification is based on computational modelling that revealed incompatibilities of the stereospecific linkages with the intermolecular oxygen-mediated hydrogen bond network. | 2021-07-29 |
20210230591 | METHODS OF REDUCING VIRUS MOLECULE LEVELS - Methods of reducing levels of virus molecules and/or treating viral infections include contacting cells with an oligonucleotide inhibitor that targets a cellular host factor that is a target RNA or target protein involved in viral replication. A pharmaceutical composition can include such an oligonucleotide inhibitor in an amount effective for treating an infection, such as a hepatitis B infection or a respiratory virus infection, such as a coronavirus infection. | 2021-07-29 |
20210230592 | Targeted Self-Assembly of Functionalized Carbon Nanotubes on Tumors - Provided herein are single wall nanotube constructs to which are convalently linked a plurality of a bifunctional chelator, a therapeutic or a diagnostic radionuclide chelated to each bifunctional chelator and a plurality of morpholino oligonucleotides conjugated to the single wall nanotube. Also provided are single wall nanotube complexes which have the morpholino oligonucleotides on the single wall nanotube constructs hybridized to other morpholino oligonucleotides. The other morpholino oligonucleotides are each conjugated to a therapeutic antibody. | 2021-07-29 |
20210230593 | PROGRAMMABLE CONDITIONAL SIRNAS AND USES THEREOF - Disclosed herein are programmable, conditionally activated small interfering RNA constructs (Cond-siRNAs) and methods of making and using the same as therapeutic agents. The Cond-siRNA comprises a sensor strand, a core strand, and a guide strand, which crossover to form a sensor duplex and a RNAi duplex attached to each other to form a single structure. Upon binding an input strand to the sensor strand, the Cond-siRNA is activated and releases RNAi targeting a desired gene. | 2021-07-29 |
20210230594 | COMPOSITIONS AND METHODS FOR CONTROLLING LEPTINOTARSA - Disclosed herein are methods of controlling insect pests, in particular | 2021-07-29 |
20210230595 | BIODEGRADABLE AND CLINICALLY-COMPATIBLE NANOPARTICLES AS DRUG DELIVERY CARRIERS - The present invention relates to the composition of a nanoparticle based on a magnesium salt, and methods of drug delivery using the nanoparticle. A preferred embodiment uses magnesium phosphate, with or without a shell to deliver aiRNA and/or siRNA. The nanoparticles of the present invention are also effective when administered orally. | 2021-07-29 |
20210230596 | RIG-I Agonists and Methods of Using Same - The present invention provides RIG-I agonists. In certain embodiments, the agonists of the invention can be used to induce a type I interferon response in a cell. | 2021-07-29 |
20210230597 | MICRO-RNAS AND COMPOSITIONS COMPRISING SAME FOR THE TREATMENT AND DIAGNOSIS OF SEROTONIN-, ADRENALIN-, NORADRENALIN-, GLUTAMATE-, AND CORTICOTROPIN-RELEASING HORMONE- ASSOCIATED MEDICAL CONDITIONS - A method of treating a bipolar disorder in a subject in need thereof is disclosed. The method comprising administering to the subject sa therapeutically effective amount of a miR-135, a precursor thereof or a nucleic acid molecule encoding said miR-135 or said precursor thereof, thereby treating the bipolar disorder. Methods of diagnosing a mood disorder in a human subject and of monitoring treatment of an anti-depressant drug or a medicament for the treatment of a mood disorder are also disclosed. | 2021-07-29 |
20210230598 | OLIGONUCLEOTIDES FOR CONTROLLING TAU SPLICING, AND USES THEREOF - The present invention provides a tau exon 10 skipping-promoting antisense oligonucleotide containing at least one 2′-O, 4′-C-ethylene-bridged nucleic acid, and a nucleotide sequence complementary to a sequence consisting of at least 10 continuous nucleotides in a region consisting of the nucleotide sequence shown in SEQ ID NO: 44 in exon 10 of a tau mRNA precursor. In addition, the present invention provides a tau exon 10 skipping-suppressing antisense oligonucleotide containing at least one 2′-O, 4′-C-ethylene-bridged nucleic acid, and a nucleotide sequence complementary to a sequence consisting of at least 10 continuous nucleotides in a region consisting of the nucleotide sequence shown in SEQ ID NO: 45 in intron 10 of a tau mRNA precursor. | 2021-07-29 |
20210230599 | STEM-LOOP COMPOSITIONS AND METHODS FOR INHIBITING INTERLEUKIN-8 - The application discloses methods and compositions for inhibiting functions associated with Interleukin-8 (IL8). The methods and compositions may involve the use of aptamers for binding to IL8 and preventing or reducing association of IL8 with CXCR1, CXCR2, or both. The methods and compositions may include one or more aptamers that bind to an N-terminal domain of IL8. The methods and compositions may include one or more aptamers that bind to a hydrophobic pocket of IL8. The methods and compositions may include one or more aptamers that bind to an N-loop of IL8. The methods and compositions may include one or more aptamers that bind to a GAG binding site of IL8. The application further provides anti-IL8 aptamers for the treatment of ocular diseases or disorders. In some cases, the anti-IL8 aptamers may have a stem-loop secondary structure. | 2021-07-29 |
20210230600 | NUCLEIC ACID-DRUG COMPLEX AND USE THEREOF - A nucleic acid-drug complex is provided in the present disclosure, which includes a nucleic acid sequence of an anti-PD-L1 aptamer and a CpG oligonucleotide sequence capable of activating TLR9, in which the CpG oligonucleotide sequence consists of a first fragment and a second fragment, and the nucleic acid sequence of the anti-PD-L1 aptamer is inserted between the first fragment and the second fragment. | 2021-07-29 |
20210230601 | HIGHLY KNOTTED MOLECULAR TOPOLOGIES FROM SINGLE-STRANDED NUCLEIC ACIDS - In some embodiments, complex molecular knots with high crossing numbers are achieved by folding, following a prescribed folding order, single-stranded DNA or RNA of customized sequences into target shapes. Such complex molecular knots with high crossing numbers are useful for biomedical applications including use as immunostimulatory agents and/or protein hosts and carriers. | 2021-07-29 |
20210230602 | COMPOSITION FOR INHIBITING CTGF EXPRESSION - A composition according to an embodiment of the present invention includes nucleic acid molecules which are capable of effectively inhibiting the expression level of connective tissue growth factor (CTGF) and collagen by RNA interference (RNAi), thereby preventing or treating a variety of fibroproliferative diseases due to overexpression of CTGF or collagen. | 2021-07-29 |
20210230603 | RNAi-MEDIATED INHIBITION OF HIF1A FOR TREATMENT OF OCULAR ANGIOGENESIS - RNA interference is provided for inhibition of HIF1A mRNA expression for treating patients with ocular angiogenesis, particularly for treating retinal edema, diabetic retinopathy, sequela associated with retinal ischemia, posterior segment neovascularization (PSNV), and neovascular glaucoma, and for treating patients at risk of developing such conditions. | 2021-07-29 |
20210230604 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GLYCOLATE OXIDASE (HAO1) BY DOUBLE-STRANDED RNA - This invention relates to compounds, compositions, and methods useful for reducing Glycolate Oxidase (HAO1) target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents. | 2021-07-29 |
20210230605 | USE OF MIR-132 AND MIR-212 IN PREPARATION OF DRUG FOR TREATING ADDICTION - The disclosure provides a non-therapeutic method for regulating DAT gene expression in a cell, which includes the step of increasing or decreasing an amount of a miR-132/212 in the cell; further provides a use of the above miRNA, an enhancer thereof or an attenuator thereof in preparing a drug for regulating uptake of dopamine by a cell; and an application of the above miRNA, the enhancer thereof or the attenuator thereof in preparing a drug for treating psychoactive substance addiction. | 2021-07-29 |
20210230606 | METHODS AND COMPOSITIONS FOR ENHANCING FUNCTIONAL MYELIN PRODUCTION - A method of generating a cell that enhances functional myelin production is provided, the method including genetically modifying the cell such that: (i) an endogenous PLP1 gene is modified to decrease its ability to inhibit myelin production; (ii) an endogenous PLP1 genetic regulatory element is modified to decrease its ability to promote PLP1 expression; (iii) an endogenous PLP1 genetic regulatory element is modified to increase its ability to inhibit PLP1 expression; or (iv) an endogenous PLP1 gene product or a PLP1 regulatory element gene product that promotes PLP1 expression is modified to decrease the PLP1 expression level, wherein the cell produces functional myelin. | 2021-07-29 |
20210230607 | METHOD FOR RECONSTRUCTING COMPLEX BIOLOGICAL SYSTEM ON THE BASIS OF POLYPROTEIN, AND USE THEREOF IN HIGH ACTIVITY SUPER SIMPLIFIED NITROGEN FIXATION SYSTEM CONSTRUCTION - An expression method, a vector and a vector composition are provided. In particular, a method for exogenously expressing a complex biological system in host cells, as well as a vector and a vector composition for the method are provided. | 2021-07-29 |
20210230608 | GYCOMODULE MOTIFS AND USES THEREOF - A nucleotide sequence encoding a glycomodule motif having a sequence selected from SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, SEQ ID NO 5 and a functionally equivalent variant thereof is provided. Also provided are an expression cassette having at least one nucleotide sequence encoding a glycomodule motif, a vector and host cell and a method for expressing a protein of interest. | 2021-07-29 |
20210230609 | Solute Carrier Family 14 Member 1 (SLC14A1) Variants And Uses Thereof - The disclosure provides nucleic acid molecules, including cDNA, comprising an alteration that encodes variant human Solute Carrier Family 14 Member 1 (SLC14A1) proteins that associate with protection against coronary artery disease (CAD). The disclosure also provides methods for classifying subjects at risk of developing a coagulation condition, based on the identification of such alterations. | 2021-07-29 |
20210230610 | A MICROORGANISM PRODUCING A MYCOSPORINE-LIKE AMINO ACID AND A METHOD FOR PRODUCING A MYCOSPORINE-LIKE AMINO ACID USING THE SAME - The present disclosure relates to a microorganism producing a mycosporine-like amino acid and a method for producing a mycosporine-like amino acid using the microorganism. | 2021-07-29 |
20210230611 | Systems and Methods for Identifying and Expressing Gene Clusters - Methods for identifying biosynthetic gene clusters that include genes for producing compounds that interact with specific target proteins are disclosed. Some methods relate to bioinformatics methods for identifying and/or prioritizing biosynthetic gene clusters. Related systems, components, and tools for the identification and expression of such gene clusters are also disclosed. | 2021-07-29 |
20210230612 | OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS - The invention relates to recombinant | 2021-07-29 |
20210230613 | FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION - The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD | 2021-07-29 |
20210230614 | FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION - The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD | 2021-07-29 |
20210230615 | Gene Targeting - Methods, reagents and compositions for providing more accurate and reliable genetic modification are provided. In particular a nucleic acid encoding a fusion protein comprising an endonuclease domain and a binding domain for an origin of replication is described. Also provided are methods, reagents and compositions for in vivo genetic modification of the genome of a non-animal cell or organism. Furthermore, the present application relates to uses of the said methods, reagents and compositions for introducing desirable traits to non-animal organisms or ameliorating or removing non-desirable traits in these organisms including in the treatment of disease. | 2021-07-29 |
20210230616 | METHODS FOR ISOLATING CELLS WITHOUT THE USE OF TRANSGENIC MARKER SEQUENCES - The present invention relates to methods for targeted editing in a plant, a plant cell or material, which is combined with the parallel introduction of a phenotypically selectable trait. Furthermore, methods are provided not comprising a step of introducing a transgenic selection marker sequence. | 2021-07-29 |
20210230617 | OPTIMAL SOYBEAN LOCI - As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences. | 2021-07-29 |
20210230618 | PLANT REGULATORY ELEMENTS AND METHODS OF USE THEREOF - The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants. | 2021-07-29 |
20210230619 | MEIOTIC PROMOTORS AND USES THEREOF - The present invention relates to new transcriptional promoters that are functional during meiosis. It thus provides new tools, methods and compositions for the expression of proteins during meiosis in a eukaryotic cell, and more particularly in a plant cell. | 2021-07-29 |
20210230620 | PLANT REGULATORY ELEMENTS AND USES THEREOF - The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided. | 2021-07-29 |
20210230621 | PLANT REGULATORY ELEMENTS AND USES THEREOF - The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided. | 2021-07-29 |
20210230622 | PLANT REGULATORY ELEMENTS AND METHODS OF USE THEREOF - The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants. | 2021-07-29 |
20210230623 | METHODS OF ALTERING SEED WEIGHT AND SEED OIL CONTENT BY MANIPULATING ALPHA-CARBOXYL-TRANSFERASE (A-CT) ACTIVITY VIA CARBOXYL-TRANSFERASE INTERACTION (CTI) PROTEIN EXPRESSION - A method of altering fatty acid and/or triacylglycerol production in plants and/or algae is provided. The method includes altering activity levels of alpha-carboxyltransferase (α-CT), a catalytic subunit of acetyl-CoA carboxylase (ACCase) by modulating an expression of at least one carboxyltransferase interaction (CTI) gene encoding at least one CTI protein. | 2021-07-29 |
20210230624 | VECTOR FOR CO-EXPRESSING GENES OF INTEREST - An expression vector for the co-expression of different polynucleotides in microalgal cells and to the expression cassettes having both polynucleotides is provided. Also provided are a host cell, a method for expressing the proteins of interest and a method for selecting a cell co-expressing two proteins of interest in a stoichiometric manner. | 2021-07-29 |
20210230625 | POLYNUCLEOTIDE SEQUENCES AND PROTEINS ENCODED THEREBY USEFUL FOR MODIFYING PLANT CHARACTERISTICS - The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions. | 2021-07-29 |
20210230626 | PLANTS WITH INCREASED YIELD AND METHOD FOR PRODUCING SAID PLANTS - The invention relates to a method for increasing the yield and biomass of a plant, by means of an increase in the expression of the L-aspartate oxidase in the plant. The method according to the invention allows an increase in the photosynthetic capacities of the plants as a result of an increase in the quantities of NAO and the derivatives thereof in said plants. The invention relates to the plants produced by such a method. | 2021-07-29 |
20210230627 | METHODS AND COMPOSITIONS RELATED TO IMPROVED NITROGEN USE EFFICIENCY - The present disclosure provides products, compositions, and methods for improving nitrogen use efficiency in tobacco plants, including e.g., Burley tobacco. This disclosure further provides genetic markers for tracking enhanced nitrogen use efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen use efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen use efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen use efficiency. | 2021-07-29 |
20210230628 | ISOLATED POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING ABIOTIC STRESS TOLERANCE, BIOMASS AND YIELD OF PLANTS - Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 552-897 and 6029-10629, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-551 and 898-6028, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant. | 2021-07-29 |
20210230629 | CAMELIZATION OF A HUMAN VARIABLE DOMAIN BY GENE CONVERSION - This disclosure provides, among other things, a transgenic animal that uses gene conversion for antibody diversification comprising B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a human heavy chain variable domain; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the human heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene and encode variable domains that have camelizing amino acid substitutions. | 2021-07-29 |
20210230630 | INDUCIBLE MODIFICATION OF A CELL GENOME - The present disclosure is directed, in some embodiments, to compositions and methods for inducible modification of a cell genome. | 2021-07-29 |
20210230631 | GENE THERAPY FOR CNS DEGENERATION - The present disclosure relates generally to compositions and methods for treating, preventing, inhibiting, or delaying central nervous system degeneration. The disclosure relates to a recombinant gene therapy vector comprising a PARK2, PINK1, DJ-1, LRRK2, SCNA, c-Rel, ATG7, VMAT2, or GBA gene, or functional fragment or variant thereof. The disclosure also relates to CRISPR/Cas-based gene editing systems for treating, preventing, inhibiting, or delaying central nervous system degeneration. | 2021-07-29 |
20210230632 | COMPOSITIONS AND METHODS FOR DELIVERY OF AAV - The disclosure provides compositions and methods for the preparation, manufacture, formulation and therapeutic use of adeno-associated virus (AAV) particles for the prevention and/or treatment of diseases. | 2021-07-29 |
20210230633 | NUCLEOTIDE VACCINE - The present invention relates to a vaccine comprising a nucleic acid construct such as a DNA construct especially a nucleic acid construct comprising sequences encoding invariant chain operatively linked to antigenic protein or peptide encoding sequences. The vaccine stimulates an immune response, especially an immune response in an MHC-I dependent, but CD4 | 2021-07-29 |
20210230634 | SYNP159, A PROMOTER FOR THE EXPRESSION OF GENES - The present invention provides an isolated nucleic acid molecule comprising, or consisting of, the nucleic acid sequence of SEQ ID NO:1 or a nucleic acid sequence of at least 400 bp having at least 80% identity to said sequence of SEQ ID NO:1, wherein said isolated nucleic acid molecule specifically leads to the expression in rod photoreceptors of a gene when operatively linked to a nucleic acid sequence coding for said gene. | 2021-07-29 |
20210230635 | ELECTROPORATION MODULES AND INSTRUMENTATION - The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and cell survival. | 2021-07-29 |
20210230636 | NUCLEIC ACID DELIVERY CARRIER, NUCLEIC ACID DELIVERY CARRIER SET, NUCLEIC ACID DELIVERY COMPOSITION, AND NUCLEIC ACID DELIVERY METHOD - According to one embodiment, a nucleic acid delivery carrier is used to integrate a first sequence into a genome of cells. The nucleic acid delivery carrier includes a donor DNA containing the first sequence, an RNA agent containing at least an RNA encoding a protein involving integration of the first sequence into the genome, and a lipid particle encapsulating the donor DNA and the RNA agent. | 2021-07-29 |
20210230637 | METHOD FOR PRODUCING HOMOZYGOUS CELLS - The purpose of the present invention is to provide a technique by which a site-specific homozygote can be efficiently acquired by increasing cross efficiency and a homozygote in a genome modification-free state can be easily obtained. A method for producing homozygous cells, said method including: (A) a step for, into cells having a heterozygous mutation at a target site, introducing heterozygouse allele specific cleavage of DNA double strand of homologous chromosomes and thus inducing crossing in the presence of a Bloom's syndrome protein inhibitor to thereby give homozygous cells at the target site; and (B) a step for selecting the homozygous cells. | 2021-07-29 |
20210230638 | SYSTEMS AND METHODS FOR THE TREATMENT OF HEMOGLOBINOPATHIES - Genome editing systems, guide RNAs, and CRISPR-mediated methods are provided for altering portions of the HBG1 and HBG2 loci in cells and increasing expression of fetal hemoglobin. | 2021-07-29 |
20210230639 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2021-07-29 |
20210230640 | METHODS FOR CONTROLLING FERMENTATION FEED RATES - The present disclosure relates to methods for enhancing productivity or yield in fermentation. The methods provide monitoring one or more compounds produced by culture cells as a feedback control for the feed rate of the fermentation. | 2021-07-29 |
20210230641 | METHODS FOR RECOVERING WATER-IMMISCIBLE ISOPRENOID COMPOUNDS FROM MICROBIAL BIOMASS - A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass. | 2021-07-29 |
20210230642 | HYDROXYLATION TECHNIQUES - The use of a cytochrome P-450 enzyme comprising SEQ ID NO: 110, or a variant enzyme having at least 70% identity thereto and having CYP-450 activity, for the hydroxylation of an organic compound, wherein the amino acid residue at position 291 is not threonine. | 2021-07-29 |
20210230643 | CONSTRUCTION OF A LACTOBACILLUS CASEI ETHANOLOGEN - An engineered bacterium for producing ethanol from one or more carbohydrates is disclosed. The bacterium can be made by (a) inactivating within a | 2021-07-29 |
20210230644 | PROCESSES FOR PRODUCING A FERMENTATION PRODUCT - The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention. | 2021-07-29 |
20210230645 | SYSTEMS AND METHODS FOR PROCESSING AND STABILIZING PLANT MATERIALS - Systems and methods are described for processing a plant material, such as cannabis and/or hemp. An example method includes: providing a slurry of plant material; providing an inert gas; adding the slurry and the inert gas to a tank; applying heat and pressure to the slurry inside the tank to generate a processed plant material; removing the processed plant material from the tank; and drying the processed plant material in a dryer to produce a dried product comprising cannabidiol (CBD). | 2021-07-29 |
20210230646 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF VANILLIN OR VANILLIN BETA-D-GLUCOSIDE - Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside. | 2021-07-29 |
20210230647 | PROCESS FOR CONTROLLING ORGANIC ACID RATIOS IN A CARBON DIOXIDE BIOCONVERSION PROCESS - A process includes providing a gaseous substrate comprising CO | 2021-07-29 |
20210230648 | ARGININE SUPPLEMENTATION TO IMPROVE EFFICIENCY IN GAS FERMENTING ACETOGENS - The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways. | 2021-07-29 |
20210230649 | Production of Carbon-Based Compounds from Cellulosic Feedstock Fermentation - Provided herein are methods of producing carbon-based compounds from the fermentation of cellulosic feedstocks. In certain embodiments, the cellulosic feedstock and/or source of the cellulosic feedstock is grass, guar gum, leaves, cattails, and/or phragmites. | 2021-07-29 |
20210230650 | METHOD FOR THE PRODUCTION OF TRIACYLGLYCERIDES AND FATTY ACIDS - The disclosure pertains to a method for the production of triacylglycerides (TAGs or Triacylglyerols) and fatty acids by the recombinant expression of a Δ11 fatty acid desaturase in protists. | 2021-07-29 |
20210230651 | Hydroformylated Triglycerides and Uses Thereof - This disclosure provides methods for the chemical modification of triglycerides that are highly enriched in specific fatty acids and subsequent use thereof for producing functionally versatile polymers. | 2021-07-29 |
20210230652 | SYSTEMS, METHODS AND COMPOSITIONS FOR THE GENERATION NOVEL HIGH YIELDING WAXES FROM MICROALGAE - The invention relates to the chemical synthesis of waxes. Specifically, the invention relates to systems and methods for the high-yield production of novel and high-value waxes in genetically-modified algae-based systems as a replacement for petroleum-based products. | 2021-07-29 |