33rd week of 2022 patent applcation highlights part 29 |
Patent application number | Title | Published |
20220259568 | COMPOSITIONS AND METHODS FOR ENHANCED GENE EXPRESSION IN CONE CELLS - The present disclosure provides polynucleotide cassettes, expression vectors and methods for the expression of a gene in cone cells. | 2022-08-18 |
20220259569 | METHODS FOR GROWING AFRICAN SWINE FEVER VIRUS IN FETAL PORCINE LUNG ALVEOLAR MACROPHAGE CELLS - A method for generating progeny of an African swine fever (ASF) virus includes providing an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating the ASF virus, wherein the cell is cultured for at least 5 passages; exposing the cell to the ASF virus; and allowing the ASF virus to replicate in the cell; thereby generating progeny of the ASF virus. | 2022-08-18 |
20220259570 | Genetically Engineered Bacteriophage - A method of engineering bacteriophages comprising isolating a bacteriophage; removing all attachment genes from a genome of said bacteriophage; inserting a first unique open reading frame encoding one or more attachment genes and inserting a second unique open reading frame encoding one or more genes useful for overcoming bacterial defenses; and inserting a non-natural attachment gene into said first open reading frame, wherein said non-natural attachment gene is specific for attaching to a selected bacteria. | 2022-08-18 |
20220259571 | HOST-INDEPENDENT EXPRESSION OF BACTERIOPHAGES - The present invention relates to a method for producing a bacteriophage in a cell-free host-independent expression system and a corresponding composition comprising a cell lysate of an organism which is different to the host of the bacteriophage, a at least one bacteriophage-host specific factor and a genome of a bacteriophage. The respective kits are also encompassed. The invention moreover refers to a bacteriophage obtained by the method of the invention and uses thereof. | 2022-08-18 |
20220259572 | ADENO-ASSOCIATED VIRUS (AAV) PRODUCTION - The disclosure relates to methods for producing an adeno-associated virus (AAV) in an E1 complementary producer cell. | 2022-08-18 |
20220259573 | METHOD FOR PRODUCING A MODIFIED BACTERIOPHAGE WITHOUT GENOME MODIFICATION - The invention relates to a method for producing a modified bacteriophage in a cell-free expression system wherein the expression of at least one gene of interest is suppressed by a molecule specifically inhibiting its expression. The invention further relates to a composition and a kit for producing a modified bacteriophage. Moreover, the invention relates to a bacteriophage which is not modified on the genomic level but on the proteomic level and its use for therapy, for diagnostic and detection assays. | 2022-08-18 |
20220259574 | ATP-INDEPENDENT BIOLUMINESCENT REPORTER VARIANTS TO IMPROVE IN VIVO IMAGING - Provided herein are chemically modified luciferase substrates for spectrally shifted emission and enhanced water solubility. Provided herein are engineered luciferases. Moreover, provided herein are new ATP-independent bioluminescent reporters which have improved biochemical and photophysical properties and are expected to have broad applications. Finally, provided herein are spectral-resolved triple-color bioluminescent systems, suitable for flexible and convenient approaches to monitor multiple biological events in either qualitative or quantitative manners | 2022-08-18 |
20220259575 | POLYPEPTIDE HAVING LUCIFERASE ACTIVITY - A novel luciferase with a small molecular weight is provided. A polypeptide with luciferase activity comprising an amino acid sequence (A) or (B) is provided: | 2022-08-18 |
20220259576 | BIO-ENGINEERED HYPER-FUNCTIONAL "SUPER" HELICASES - Conformationally-constrained helicases having improved activity and strength are provided. Methods of making conformationally-constrained helicases having improved activity and strength are provided. Methods of using conformationally-constrained helicases having improved activity and strength are provided. | 2022-08-18 |
20220259577 | CHONDROSULPHATASE AND USE THEREOF - Provided are chondrosulfatase and a use thereof, belonging to the technical field of biological engineering. Chondrosulfatase is screened and identified from the natural world, the maximum similarity between its amino acid sequence and that of the chondrosulfatase reported by NCBI being 85%; then the expression in | 2022-08-18 |
20220259578 | MATERIALS AND METHODS FOR TREATMENT OF HEMOGLOBINOPATHIES - Materials and methods for treating a patient with hemoglobinopathy, both ex vivo and in vivo and materials and methods for deleting at least a portion of a human beta globin locus on chromosome 11 in a human cell by genome editing and thereby increasing the production of fetal hemoglobin (HbF). | 2022-08-18 |
20220259579 | ENGINEERED HUMAN EXTRACELLULAR DNASE ENZYMES - The present disclosure provides a library of engineered DNASE proteins (including DNASE1, DNASE1-LIKE 1, DNASE1-LIKE 2, DNASE1-LIKE 3, DNASE2A, DNASE2B) that allows to select drug candidates for developing therapeutics for treating conditions characterized by neutrophil extracellular trap (NET) accumulation and/or release. In accordance with the invention, the selected DNase variant has improved properties, including properties amenable to clinical development, including manufacturing, toxicology, pharmacokinetic, and/or use in therapy. | 2022-08-18 |
20220259580 | ENZYMATIC HEXOSAMINIDATION OF LACTOSE - The present disclosure relates to methods for producing human milk oligosaccharide (HMO) core structures using glycosidases from family GH20 hexosaminidases. In particular, the present disclosure provides methods for producing lacto-N-triose II (LNT II) and/or lacto-N-tetraose (LNT) by reacting glucosamine-oxazoline and/or lacto-N-biose-oxazoline with lactose catalysed by an enzyme of the glycoside hydrolase family 20 (GH20) according to the classification of the Carbohydrate-Active-Enzymes (CAZy) database. Specific optimized enzymes are identified to catalyse the reactions. | 2022-08-18 |
20220259581 | ENGINEERED SYNTHASE FOR PRODUCTION OF TRYPTOPHAN DERIVATIVES AND INTRANSIGENT SUBSTRATES - This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids. | 2022-08-18 |
20220259582 | RuBisCO PROTEIN FIBERS - Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, tbr example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 μm filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers. | 2022-08-18 |
20220259583 | MAGNETIC PARTICLE FOR MODULATING ACTIVITY OF CELL - The magnetic particle for modulating the activity of cells according to an exemplary embodiment of the present invention is capable of specifically binding to a bioactive material, and includes a core and a plurality of nanoparticles disposed on the surface of the core, and may generate a torque of 10 pN·nm or more when a rotating magnetic field of 5 mT is applied. | 2022-08-18 |
20220259584 | MAGNETIC PARTICLE COMPOSITION, USE OF MAGNETIC PARTICLE COMPOSITION FOR NUCLEIC ACID SEPARATION, KIT FOR OBTAINING MAGNETIC PARTICLE COMPOSITION, MAGNETIC PARTICLES, CHAOTROPIC SALT, AND SEPARATION AND PURIFICATION METHOD - A magnetic particle composition (e) containing magnetic particles (c) and a chaotropic salt (D), the magnetic particles (c) each including a core particle (P) that is a magnetic silica particle containing a magnetic metal oxide particle (A), wherein the magnetic metal oxide particle (A) in the core particle (P) has a weight percentage of 60 wt % or more based on the weight of the core particle (P), and the magnetic particles (c) have a particle size distribution with a coefficient of variation of 5 to 50%. | 2022-08-18 |
20220259585 | METHODS OF MAKING AND UTILIZING AMBER-OBLIGATED PHAGE DISPLAY LIBRARIES - Embodiments of the present disclosure pertain to methods of constructing a phage display library where at least 90% of combinatorial regions in the phage display library include at least one in-frame amber codon. Further embodiments of the present disclosure pertain to the formed phage display libraries. Additional embodiments of the present disclosure pertain to methods of selecting peptides or proteins that bind to a desired target (e.g., a ligand binding site of a desired target) by utilizing the phage display libraries of the present disclosure. Further embodiments of the present disclosure pertain to peptides that have been screened from the phage display libraries and methods of the present disclosure, such as inhibitors of sirtuin 2, or inhibitors of ENL. | 2022-08-18 |
20220259586 | SINGLE CELL ANALYSIS OF TRANSPOSASE ACCESSIBLE CHROMATIN - Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions. | 2022-08-18 |
20220259587 | METHODS FOR GENERATING, AND SEQUENCING FROM, ASYMMETRIC ADAPTORS ON THE ENDS OF POLYNUCLEOTIDE TEMPLATES COMPRISING HAIRPIN LOOPS - A method of generating an asymmetric closed-ended double stranded nucleic acid template from a double stranded nucleic acid template having free 5′ and 3′ ends by use of hairpin or dumbbell adaptors, and sequencing therefrom. | 2022-08-18 |
20220259588 | TREATING AND PREVENTING MICROBIAL INFECTIONS - The invention provides methods for treating or preventing microbial (eg, bacterial) infections and means for performing these methods. In particular, treatment of infections requiring rapid and durable therapy is made possible, such as for treating acute conditions such as septicemia, sepsis, SIRS or septic shock. The invention is particularly useful, for example, for treatment of microbes such as for environmental, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system. The invention also useful for the treatment of pathogenic bacterial infections in subjects receiving a treatment for a disease or condition, such as a transplant or a treatment for cancer, a viral infection or an autoimmune disease. | 2022-08-18 |
20220259589 | REVERSIR TM COMPOUNDS - The present invention relates, in general to agents that modulate the pharmacological activity of conjugated siRNAs. | 2022-08-18 |
20220259590 | Systems and Methods for Designing RNA Nanostructures and Uses Thereof - Systems and methods for generating RNA nanostructures capable of linking RNA structures and capable of securing aptamers in an active and stable structure are disclosed. Generally, RNA possesses many structural properties to create novel nanostructures and machines. RNA tertiary structure is composed of discrete and recurring components known as tertiary ‘motifs’. Along with the helices that they interconnect, many of these structural motifs appear highly modular. Systems and methods herein generate a motif library including canonical and noncanonical motifs to design a candidate path to connect one or more RNA molecules. These paths can also be used to secure RNA aptamers to improve aptamer stability and activity. | 2022-08-18 |
20220259591 | ANTI-INFLAMMATORY TREATMENT VIA INHIBITION OF ENDOTHELIAL CELL KINESIN LIGHT CHAIN 1, VARIANT 1 (KLC1C) - Provided herein are compositions and methods for the inhibition of endothelial cell kinesin light chain 1, variant 1 (KLC1C) expression and/or activity, and treatment or prevention of inflammation therewith. | 2022-08-18 |
20220259592 | EXON SKIPPING COMPOSITIONS FOR TREATING MUSCULAR DYSTROPHY - Antisense molecules capable of binding to a selected target site in the human dystrophin gene to induce exon 53 skipping are described. | 2022-08-18 |
20220259593 | CONTROL OF MAMMALIAN GENE DOSAGE USING CRISPR - The present disclosure provides methods and compositions for precisely controlling the expression levels of mammalian genes using CRISPRi or CRISPRa and one or more modified sgRNAs. The methods and compositions are useful for, inter alia, titrating the expression of a gene of interest, identifying drug targets and mechanisms of drug resistance, and enabling the analysis of and control over metabolic and signaling pathway fluxes. | 2022-08-18 |
20220259594 | VECTORS AND COMPOSITIONS FOR TREATING HEMOGLOBINOPATHIES - The invention provides improved gene therapy vectors, compositions, and methods. | 2022-08-18 |
20220259595 | FUNCTIONAL NUCLEIC ACID MOLECULES UPREGULATING THE TRANSLATION OF A FRATAXIN MRNA - There are disclosed functional nucleic acid molecules comprising at least one target binding sequence comprising a sequence reverse complementary to a frataxin mRNA sequence; and a regulatory sequence comprising a SINE B2 element or a functionally active fragment of a SINE B2 element or an internal ribosome entry site (IRES) sequence or an IRES derived sequence. | 2022-08-18 |
20220259596 | Inhibitors of microRNA 451a for Treatment of Endometriosis - The invention includes compositions and methods for the treating or preventing endometriosis in a subject in need thereof. In one aspect, the invention relates to compositions and methods for inhibiting microRNA451a. | 2022-08-18 |
20220259597 | OLIGONUCLEOTIDE ANTAGONISTS FOR RNA GUIDED GENOME EDITING - Compositions and methods for inactivating RNA-guided genome editing systems in specific tissue, for example hepatocytes, are provided herein. In one embodiment, the compositions are small chemically modified oligonucleotides that can target and bind to guide RNA, thus eliminating the ability of guide RNA to interact with an endonuclease. The disclosed oligonucleotides are delivered in lipid nanoparticles formulated to target a specific tissue. Subsequently delivered RNA-guided genome editing systems will be inhibited in the specific tissue that received the oligonucleotides. The disclosed compositions and methods allow for reduced RNA-guided genome editing in hepatocytes. | 2022-08-18 |
20220259598 | NOVEL DNA APTAMERS AND USE THEREOF - The present disclosure relates to novel DNA aptamers and use thereof. In particular, the present disclosure relates to DNA aptamers selected from a DNA library using Cell-SELEX to bind specifically to cancer cells. The DNA aptamers of the present disclosure selected and optimized for high binding affinity to cancer cells can be effectively used for the diagnosis of cancer as they have enhanced targeting efficiencies for target cells and tissues. | 2022-08-18 |
20220259599 | COMPOSITIONS AND METHODS FOR INHIBITING NUCLEIC ACID VECTOR-INDUCED INFLAMMATORY RESPONSES - Provided herein, in some embodiments, are nucleic acid vectors, such as recombinant viral genomes, comprising an inhibitory oligonucleotide that reduces inflammation for use, for example, in gene therapy. | 2022-08-18 |
20220259600 | COMPOUNDS AND METHODS FOR MODULATING TMPRSS6 EXPRESSION - Disclosed herein are compositions and compounds comprising modified oligonucleotides for modulating TMPRSS6 and modulating an iron accumulation disease, disorder and/or condition in an individual in need thereof. Iron accumulation diseases in an individual such as polycythemia, hemochromatosis or β-thalassemia can be treated, ameliorated, delayed or prevented with the administration of antisense compounds targeted to TMPRSS6. | 2022-08-18 |
20220259601 | UBE3A ANTISENSE THERAPEUTICS - The invention provides compositions useful to knock down overexpression of UBE3A and treat conditions associated with Dup15 | 2022-08-18 |
20220259602 | INCREASING ATOH1 LIFE TO DRIVE SENSORINEURAL HAIR CELL DIFFERENTIANTION - The present disclosure provides compositions and methods for treating subjects at risk for or with sensorineural hearing loss by modulating the rate of Atoh1 protein degradation to increase levels of Atoh1 protein. | 2022-08-18 |
20220259603 | METHODS AND CELLS FOR MICROBIAL PRODUCTION OF PHYTOCANNABINOIDS AND PHYTOCANNABINOID PRECURSORS - The present disclosure relates generally to methods and cell lines for the production of phytocannabinoids, phytocannabinoid precursors or intermediates, or phytocannabinoid analogue. Methods for transformation of host cells, such as yeast cells, are described. Cells may be transformed, for example, with a polynucleotide encoding a polyketide synthase (PKS) enzyme, a polynucleotide encoding an olivetolic acid cyclase (OAC) enzyme, and/or a polynucleotide encoding a prenyltransferase (PT) enzyme; and optionally a polynucleotide encoding an acyl-CoA synthase (Alk) enzyme; a polynucleotide encoding a fatty acyl CoA activating (CsAAE) enzyme; and/or a polynucleotide encoding a THCa synthase (OXC) enzyme. | 2022-08-18 |
20220259604 | MODIFIED YEAST CELLS THAT OVER-EXPRESS SELECTED ENDOGENOUS PROTEINS - The present strains and methods relate to yeast cells that over-overproduce selected endogenous proteins having a high amino acid content of a selected amino acid. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of a selected amino acid, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients. | 2022-08-18 |
20220259605 | MODIFIED CELL - The invention relates to a method of producing a chromosome-free bacterial cell having surface-displayed nanobodies, comprising: i) degrading native chromosomal DNA in a bacterial cell by expressing an endonuclease in the bacterial cell that is capable of recognition and double-stranded cutting of the native chromosomal DNA, wherein the expression of the endonuclease is under control of an inducible or repressible promoter; and ii) transforming the bacterial cell with recombinant nucleic acid encoding enzymes of one or more biochemical energy pathways, or parts thereof, which provide the chromosome-free bacterial cell with energy. The invention further relates to associated compositions, chromosome-free bacterial cells and treatments. | 2022-08-18 |
20220259606 | METHODS OF MODULATING NUCLEIC ACID STABILITY AND PROTEIN EXPRESSION - A synthetic nucleic acid which encodes a protein wherein at least one optimal or non-optimal codon in a wild type nucleic acid encoding the protein has been replaced respectively with one or more non-optimal codons or optimal codons encoding the same amino acid. | 2022-08-18 |
20220259607 | MANIPULATION OF GENES INVOLVED IN SIGNAL TRANSDUCTION TO CONTROL FUNGAL MORPHOLOGY DURING FERMENTATION AND PRODUCTION - The present disclosure provides a microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that have altered morphology and/or growth under specific growth conditions. The method and system utilize high-throughput (HTP) methods to produce filamentous fungal production strains with a desired morphological phenotype. | 2022-08-18 |
20220259608 | REGULATABLE PROMOTER - A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system. | 2022-08-18 |
20220259609 | MORPHOGENIC REGULATORS AND METHODS OF USING THE SAME - The present disclosure provides methods and compositions for generating transgene-free plants. Particularly, the transgene-free plants are regenerated by transient expression of morphogenic regulators in a plant cell that is precisely edited using a gene-editing methodology. Also, provided are transformed plants, plant cell, tissues, and seeds in which a target gene is edited, but non-transgenic. | 2022-08-18 |
20220259610 | METHOD FOR SELECTING PROMOTER THAT FUNCTIONS IN ORGANELLE - The present invention provides a method for selecting a promoter that functions in an organelle, the method comprising: (1) the step of preparing sequence information obtained by RNA sequencing analysis; (2) the step of mapping the sequence information prepared in the step (1) onto a sequence of organellar DNA; (3) the step of calculating the amount of change in RNA expression in each region based on the mapping information obtained in the step (2); (4) the step of selecting regions in which the amount of change obtained in the step (3) is within a range of preset reference values; and (5) the step of identifying a region, among the regions selected, as a promoter functioning in an organelle. | 2022-08-18 |
20220259611 | METHOD FOR INCREASING YIELD IN PLANTS - The invention relates to a method for obtaining plants presenting a higher yield by inhibition of the GDH2 activity in the plants. | 2022-08-18 |
20220259612 | METHODS AND COMPOSITIONS FOR MODIFYING CYTOKININ OXIDASE LEVELS IN PLANTS - This invention relates to compositions and methods for improving/enhancing yield traits by modifying cytokinin oxidase (CKX) levels in a plant. The invention further relates to plants produced using the methods and compositions of the invention. | 2022-08-18 |
20220259613 | ABIOTIC STRESS TOLERANT PLANTS AND METHODS - Isolated polynucleotides and polypeptides, and recombinant DNA constructs are useful for conferring improved drought tolerance and yield. Compositions (such as plants or seeds) comprise these recombinant DNA constructs; and methods utilize these recombinant DNA constructs. The recombinant DNA constructs comprise a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotides encode drought tolerance polypeptides. | 2022-08-18 |
20220259614 | INSECTICIDAL PROTEINS FROM PLANTS AND METHODS FOR THEIR USE - Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. | 2022-08-18 |
20220259615 | COTTON TRANSGENIC EVENT MON 88702 AND METHODS FOR DETECTION AND USES THEREOF - The invention provides a transgenic | 2022-08-18 |
20220259616 | A Platform for T Lymphocyte Genome Engineering and In Vivo High-Throughput Screening Thereof - The present invention includes compositions and methods for T cell genome editing and screening in vivo. In certain aspects, the invention includes an sgRNA library for genome-scale mutagenesis. | 2022-08-18 |
20220259617 | ENGINEERED HUMAN-ENDOGENOUS VIRUS-LIKE PARTICLES AND METHODS OF USE THEREOF FOR DELIVERY TO CELLS - Human-derived virus-like particles (heVLPs), comprising a membrane comprising a phospholipid bilayer with one or more HERV-derived envelope proteins on the external side; one or more HERV-derived GAG proteins in the heVLP core, and a cargo molecule, e.g., a biomolecule and/or chemical cargo molecule, disposed in the core of the heVLP on the inside of the membrane, wherein the heVLP does not comprise a gag protein, except for gag proteins that are encoded in the human genome or gag proteins that are encoded by a consensus sequence that is derived from gag proteins found in the human genome, and methods of use thereof for delivery of the cargo molecule to cells. | 2022-08-18 |
20220259618 | AGENT FOR INDUCING SPECIFIC IMMUNITY AGAINST SEVERE ACUTE RESPIRATORY SYNDROME VIRUS SARS-COV-2 IN LIQUID FORM (VARIANTS) - The invention relates to a biomolecule agent for inducing specific immunity against severe acute respiratory syndrome virus SARS-CoV-2, in liquid form, which contains a single active component, comprising the expression vector including either: the genome of the recombinant strain of human adenovirus serotype 26 or 5, wherein the E1 and E3 regions are deleted, the vector with an integrated expression cassette is selected from SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3; or the recombinant strain of simian adenovirus serotype 25, wherein the E1 and E3 regions are deleted, the vector with an integrated expression cassette selected from SEQ ID NO:4, SEQ ID NO:2, or SEQ ID NO:3. The recombinant strain of human adenovirus serotype 26 may include the ORF6-Ad26 region replaced by ORF6-Ad5. | 2022-08-18 |
20220259619 | COMPOSITIONS AND METHODS FOR TREATING LEBER'S HEREDITARY OPTIC NEUROPATHY - Disclosed herein is a recombinant nucleic acid, comprising: a mitochondrial targeting sequence; a mitochondrial protein coding sequence, wherein said mitochondrial protein coding sequence encodes a polypeptide comprising a mitochondrial protein; and a 3′UTR nucleic acid sequence. Also disclosed is a pharmaceutical composition comprising the recombinant nucleic acid and a method of treating Leber's hereditary optic neuropathy (LHON) using the pharmaceutical composition. | 2022-08-18 |
20220259620 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2022-08-18 |
20220259621 | NANOPARTICLE BIOHYBRID COMPLEXES - Disclosed herein are biohybrid protein complexes capable of using light energy to photocatalyze the reduction of N | 2022-08-18 |
20220259622 | CONSTRUCTION METHOD AND RECOMBINANT YEAST STAIN YARROWIA LIPOLYTICA FOR XYLITOL SYNTHESIS - The present invention discloses a construction method and a recombinant yeast stain | 2022-08-18 |
20220259623 | Methods And Compositions For Producing Hydrocarbons - Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels. | 2022-08-18 |
20220259624 | SELECTIVE PROCESS FOR PREPARING SULFOXIDES BY ENZYMATIC CATALYSIS - The present invention relates to a selective process for preparing sulfoxides from sulfides by enzymatic catalysis, and to a composition comprising a symmetrical sulfide, an oxidoreductase enzyme catalyzing the oxidation of said symmetrical sulfide to symmetrical sulfoxide; optionally at least one cofactor C of said enzyme E; and an oxidant, which allows in particular the implementation of said process. | 2022-08-18 |
20220259625 | GENETICALLY ENGINEERED BACTERIUM FOR SARCOSINE PRODUCTION AS WELL AS CONSTRUCTION METHOD AND APPLICATION - The disclosure discloses a genetically engineered strain for sarcosine production as well as a construction method and application. The genetically engineered strain is obtained by using | 2022-08-18 |
20220259626 | GENERATION OF ACYL AMINO ACIDS - In certain embodiments, the present invention comprises compositions and methods useful in the generation of acyl amino acids. In certain embodiments, the present invention provides an engineered polypeptide comprising a peptide synthetase domain; in some such embodiments, the engineered polypeptide comprises only a single peptide synthetase domain. In some embodiments, the present invention provides an engineered peptide synthetase that is substantially free of a thioesterase domain, and/or a reductase domain. In certain embodiments, the present invention provides an acyl amino acid composition comprising a plurality of different forms of an acyl amino acid. In some such compositions, substantially all of the acyl amino acids within the composition contain the same amino acid moiety and differ with respect to acyl moiety. We also described populations where the fatty acid is for example 95% one length (C14, myristic). | 2022-08-18 |
20220259627 | PROCESSES FOR THE PREPARATION OF (R)-2-(7-(4-CYCLOPENTYL-3-(TRIFLUOROMETHYL)BENZYLOXY)-1,2,3,4-TETRAHYDROC- YCLOPENTA[B]INDOL-3-YL)ACETIC ACID AND SALTS THEREOF - The present invention relates to processes and intermediates useful in the preparation of of (R)-2-(7-(4-cyclopentyl-3-(trifluoromethyl)benzyloxy)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl)acetic acid of Formula (Ia) and salts thereof, an S1P1 receptor modulator that is useful in the treatment of S1P1 receptor-associated disorders, for example, diseases and disorders mediated by lymphocytes, transplant rejection, autoimmune diseases and disorders, inflammatory diseases and disorders (e.g., acute and chronic inflammatory conditions), cancer, and conditions characterized by an underlying defect in vascular integrity or that are associated with angiogenesis such as may be pathologic (e.g., as may occur in inflammation, tumor development and atherosclerosis). | 2022-08-18 |
20220259628 | IMMOBILIZED ENZYME COMPOSITIONS FOR THE PRODUCTION OF HEXOSES - The invention relates to immobilized enzyme compositions for the preparation of a hexose. Hexoses include, for example, tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gulose, idose, and inositol. The invention also relates to an enzymatic process for preparing a hexose from a saccharide by contacting a starch derivative with an immobilized enzyme composition of the invention. | 2022-08-18 |
20220259629 | RHAMNOSE-POLYSACCHARIDES - The present invention relates to a method of synthesizing a rhamnose polysaccharide. The invention also relates to a synthetic streptococcal polysaccharide, a streptococcal glycoconjugate, an immunogenic composition or vaccine comprising the streptococcal polysaccharide or glycoconjugate and the polysaccharide, glycoconjugate, immunogenic composition or vaccine for use in raising an immune response in an animal or for use in treating or preventing a disease, condition or infection with a streptococcal aetiology. | 2022-08-18 |
20220259630 | IMPROVED METHOD FOR PRODUCING ISOMALTOOLIGOSACCHARIDES - Described is a method for producing improved isomalto-oligosaccharides (IMO) from maltodextrins. The improved method involves the complete or partial replacement of β-amylase, as used in a conventional method, with a selected α-amylase. The resulting IMO have longer chain-length and reduced residual glucose content compared to IMO produced using a conventional method. | 2022-08-18 |
20220259631 | PRODUCTION OF FUCOSYLLACTOSE IN HOST CELLS - This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of fermentation of metabolically engineered host cells. The disclosure describes a method of producing fucosyllactose by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for an enzyme involved in fucosyllactose synthesis; more specifically, the cell comprises a nucleic acid sequence coding for a fucosyltransferase, thereby synthesizing fucosyllactose and at least one nucleic acid expressing a membrane protein, more specifically, a nucleic acid sequence expressing a membrane protein enabling fucosyllactose transport. | 2022-08-18 |
20220259632 | METHOD OF OLIGONUCLEOTIDE SYNTHESIS - The invention relates to methods and kits for the synthesis of oligonucleotides via controlled, localised deprotection of 3′-ONH | 2022-08-18 |
20220259633 | METHOD FOR PRODUCING MONOPHOSPHORYL LIPID A - Provided is a method of producing monophosphoryl lipid A (MPLA). According to the method, MPLA may be produced with high purity and high purity by using a bacterium producing MPLA. | 2022-08-18 |
20220259634 | GENE INVOLVED IN SYNTHESIS OF CYCLIC PEPTIDE COMPOUND, METHOD FOR PRODUCING CYCLIC PEPTIDE COMPOUND USING THE SAME, AND TRANSFORMANT COMPRISING THE SAME - This invention is intended to identify a gene cluster involved in biosynthesis of a cyclic peptide compound produced by a filamentous fungus of the | 2022-08-18 |
20220259635 | COMPOSITION AND METHODS FOR PROCESSING A SPUTUM SAMPLE - The present disclosure relates to a composition and its use for treating a sputum sample suspected to contain mycobacteria. The composition comprises thymol, a linear or branched alcohol, a chaotropic agent, a reducing agent, a detergent, and a buffer, and has a pH value between 8.5 and 10. Also disclosed is a method for treating a sputum sample suspected to contain mycobacteria. | 2022-08-18 |
20220259636 | MICROBIAL COLONY DETECTION SYSTEM - Provided is a method of detecting microbial colonies, the method including: irradiating light having coherence to a sample unit in which a sample is accommodated; detecting, by an image sensor, transmitted light passing through the sample unit to obtain a sample image in a time-series manner; analyzing the sample image by a controller after a preset time to determine the concentration level of colonies in the sample; and obtaining a spatial correlation of the coherence pattern of the sample image when the concentration level determined by the controller is a high concentration that is equal to or greater than a preset reference value, and determining information about the concentration of colonies in the sample based on the change of the spatial correlation of the coherence pattern over time. | 2022-08-18 |
20220259637 | METHOD FOR ENRICHING NUCLEIC ACIDS BY SIZE - The present invention provides a poly(alkylene oxide) polymer based size selective method for enriching nucleic acid molecules having a length below a cut-off value from a nucleic acid containing sample, the method comprising: (a) preparing a binding mixture comprising —the nucleic acid containing sample, —a poly(alkylene oxide) polymer and —a salt and binding nucleic acid molecules of different sizes to a solid phase which comprises a functional group, preferably carboxylated magnetic particles; (b) separating the solid phase with the bound nucleic acid molecules from the remaining sample; and (c) contacting the solid phase with the bound nucleic acid molecules at least once with an elution composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute nucleic acid molecules having a length below the cut-off value from the solid phase while larger nucleic acid molecules having a length above the cut-off value remain bound to the solid phase, wherein the concentration (w/v) of the poly(alkylene oxide) polymer in the elution composition is lower than the concentration (w/v) of the poly(alkylene oxide) polymer in the binding mixture of (a); (d) separating the solid phase with the bound larger nucleic acid molecules from the eluted nucleic acid molecules; and (e) optionally further purifying the eluted nucleic acid molecules. The method is particularly useful for separating extracellular target nucleic acids by size. | 2022-08-18 |
20220259638 | METHODS AND COMPOSITIONS FOR HIGH THROUGHPUT SAMPLE PREPARATION USING DOUBLE UNIQUE DUAL INDEXING - Provided herein are methods and compositions for high throughput sample preparation using double unique dual indexing. | 2022-08-18 |
20220259639 | Multipurpose Compositions for Collecting and Transporting Biological Material - The invention is directed to compositions and methods for collecting, transporting, and storing, without refrigeration, biological materials, which may comprise samples of biological, clinical, forensic, and/or environmental origin. These compositions preserve the viability of the collected organisms and/or the RNA/DNA and proteins in the sample composition mixture and permit the long-term storage of samples. Compositions are compatible with subsequent manipulation of the sample, including propagation and culture of the collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids, and other macromolecules. When the compositions containing microorganisms and any polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and/or characterize select microbes and their components, such as nucleic acids, when compared to conventional microbial transport media that contain interfering substances and RNA/DNA extraction is not required prior to PCR analysis. In particular, the compositions disclosed allow for the collection, transport and storage of biological samples for extended periods at ambient temperature, while maintaining the integrity of the macromolecules of the sample for subsequent extraction, identification, and quantitation. | 2022-08-18 |
20220259640 | METHOD FOR DIAGNOSING BRAIN TUMOR THROUGH BACTERIAL METAGENOMIC ANALYSIS - Provided is a method of diagnosing a brain tumor by analyzing the increase or decrease in content of specific bacteria-derived extracellular vesicles by performing bacterial metagenomic analysis using a normal individual and a subject-derived sample, which makes it possible to diagnose and predict a risk group for a brain tumor early, to delay or prevent the onset of the disease through appropriate management, and even after the onset of a brain tumor, early diagnosis can be performed to reduce the incidence of the brain tumor, a therapeutic effect cannot be only increased, and the disease can be improved and recurrence thereof can be prevented by avoiding exposure to causative factors through metagenomic analysis in patients diagnosed with a brain tumor. | 2022-08-18 |
20220259641 | AMPLIFICATION ASSAY FOR THE DETECTION OF ANAPLASMA PHAGOCYTOPHILUM - Methods, primers, and kits for detecting | 2022-08-18 |
20220259642 | METHOD FOR DETERMINING THE RISK OF AN AVIAN PATHOGENIC E. COLI (APEC) INFECTION IN AN AVIAN FLOCK - The invention pertains to a method for determining the risk of an Avian Pathogenic | 2022-08-18 |
20220259643 | METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS - Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. | 2022-08-18 |
20220259644 | SYSTEM AND METHOD FOR CAPTURING AND ANALYZING CELLS - A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells. | 2022-08-18 |
20220259645 | RNA Replication Using Transcription Polymerases - Compositions and methods for amplifying RNA by replication using transcription polymerases are disclosed. Such replicated RNAs can be used in various applications such as RNAi therapeutics, diagnostic probes, RNA sequencing, directed evolution of RNA aptamers without intermediate conversion to DNA, and RNA vaccines. The transcription polymerases comprise T7 bacteriophage RNA polymerase. | 2022-08-18 |
20220259646 | COMPOSITIONS AND METHODS OF LABELING NUCLEIC ACIDS AND SEQUENCING AND ANALYSIS THEREOF - Compositions and methods labeling individual nucleic acid (e.g., DNA) molecules with a unique molecular identifier (UMI), followed by amplification by PCR are provided. The PCR amplicons can be grouped by the UMI they contain and traced back to the original molecule. More specifically, the grouped reads with the same UMI represent one original nucleic acid (e.g., DNA) molecule, meaning they share the same nucleic acid sequence. Methods of sequencing the labeled nucleic acid are also provided. The methods can include determination of a consensus sequence, which thus eliminates errors that may be introduced in the amplification and sequencing process. Such methods can be used in, for example, the detection of rare genetic variants. | 2022-08-18 |
20220259647 | METHODS OF DETECTING DISEASE AND TREATMENT RESPONSE IN cfDNA - Methods are provided for detecting/generating cell-free DNA (cfDNA) profiles from a sample, e.g., plasma, urine, or both. The disclosure also provides methods of detecting disease in a subject, including detecting tissue types and subtypes based on the cfDNA profiles generated. In certain specific aspects, the methods disclosed provide for the detection of diseases, such as, cancer, diabetes, hypertension, etc. and also detect the responsiveness of a subject to treatment, and/or progression of such diseases. | 2022-08-18 |
20220259648 | METHODS AND COMPOSITIONS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES - Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide. | 2022-08-18 |
20220259649 | METHOD FOR TARGET SPECIFIC RNA TRANSCRIPTION OF DNA SEQUENCES - Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target including using hairpin adaptors having a double stranded promoter and an overhang which hybridizes with a reverse-complementary overhang on a target nucleic acid. RNA transcription eliminates clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing. | 2022-08-18 |
20220259650 | SENSOR AND SENSING SYSTEM - A sensor includes: a substrate; a polymerase attached to the substrate; two electrodes having a space therebetween and being supported by the substrate; and a modulatable electrically conductive channel attached to the two electrodes, the modulatable electrically conductive channel including a modified, partially double stranded nucleic acid polymer electrically connected to the two electrodes and bridging the space between the two electrodes. The modified, partially double stranded nucleic acid polymer includes: a first polynucleotide chain and a second polynucleotide chain partially bonded together; a gap in the first polynucleotide chain wherein nucleotides are missing, the gap configured to at least partially receive the switch strand; and a plurality of nucleotide bases of the second polynucleotide chain exposed at the gap in the first polynucleotide chain. | 2022-08-18 |
20220259651 | METHODS FOR SEQUENCING POLYNUCLEOTIDES - Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules. | 2022-08-18 |
20220259652 | Single Cell Nucleic Acid Detection and Analysis - Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided. | 2022-08-18 |
20220259653 | Dimer avoided multiplex polymerase chain reaction for amplification of multiple targets - The present disclosure relates to methods for amplifying nucleic acids that avoid problems associated with primer-dimer formation. The present methods are referred to herein as dimer avoided multiplex polymerase chain reaction (dam-PCR). The methods disclosed herein generally comprise the steps of reverse transcribing at least one first strand of DNA, for example cDNA from an RNA sample, wherein each first strand of DNA incorporates a reverse common primer binding site; selecting each first strand of DNA; synthesizing at least one second strand of DNA from each of the at least one first strand of DNA, wherein each second strand of DNA incorporates a forward common primer binding site; selecting each second strand of cDNA; and amplifying the DNA strands using common primers. Alternatively, the method may be performed using a gDNA template. The methods described herein, due to the selection of DNA strands and removal of unused primers prior to amplification, avoid primer-dimer formation and allow for greater sensitivity and efficiency compared with conventional multiplex PCR methods. | 2022-08-18 |
20220259654 | COMPOSITIONS AND METHODS FOR XI CHROMOSOME REACTIVATION - In some aspects, the disclosure relates to the reactivation of inactive X chromosomes (Xi). In some embodiments, the disclosure provides compositions and methods for the reactivation of inactive X chromosomes. In some embodiments, the compositions and methods described by the disclosure may be useful for the treatment of dominant X-linked diseases. | 2022-08-18 |
20220259655 | METHODS FOR ASSESSING THE RISK OF DEVELOPING SEVERE CUTANEOUS ADVERSE DRUG REACTIONS INDUCED BY DISEASE-MODIFYING ANTIRHEUMATIC DRUGS, DETECTION KIT THEREOF AND USES THEREOF - A method for assessing the risk of severe cutaneous adverse drug reactions (SCARs) induced by disease-modifying antirheumatic drugs is provided, wherein the severe cutaneous adverse drug reactions comprises but not being limited to: Stevens-Johnson Syndrome (SJS), toxic epidermal necrolysis (TEN) and drug rash with eosinophilia and systemic symptoms (DRESS). Also provided is a detection kit for assessing the risk of developing cutaneous adverse drug reactions in a subject, said kit comprising a reagent for determining specific HLA alleles and a use of the detection kit in assessing the risk of developing cutaneous adverse drug reactions in a subject. | 2022-08-18 |
20220259656 | BLOOD BIOMARKER AND GENETIC MARKERS ASSOCIATED WITH RESPONSE TO CRTH2 RECEPTOR ANTAGONISTS - The present invention provides a blood biomarker and genetic markers on human chromosome 1 that are associated with a beneficial response to CRTH2 receptor antagonists. These combination of the blood biomarker and genetic markers are useful, inter alia, to identify patients who are most likely to benefit from treatment with CRTH2 receptor antagonist compositions and drug products, in methods of treating patients having a disease susceptible to treatment with a CRTH2 receptor antagonist, e.g., asthma, and in methods for selecting the most appropriate therapy for such patients. | 2022-08-18 |
20220259658 | miRNA MARKER FOR DIAGNOSIS AND/OR TREATMENT OF ALZHEIMER'S DISEASE - The disclosure provides a miRNA marker for the treatment and/or diagnosis of Alzheimer's disease (AD), and the miRNA marker is a miRNA23 cluster. The disclosure provides use of the miRNA23 cluster in the diagnosis and treatment of AD. The expression of the miRNA23 cluster is detected using primers for the microRNA marker through AD model cells, AD model animals and natural aging animals, and blood of AD patients, and it is found that the expression of the miRNA23 cluster is significantly reduced during the progression of AD, which reduces neuronal apoptosis by inhibiting the GSK-3β-mediated tau protein phosphorylation. Therefore, the miRNA23 cluster can be used as a novel biomarker and therapeutic target for the early, non-invasive diagnosis and treatment of AD. | 2022-08-18 |
20220259659 | TARGETED HYBRID CAPTURE METHODS FOR DETERMINATION OF T CELL REPERTOIRES - The present disclosure relates generally to methods for targeted hybrid capture of rearranged T cell receptors. More particularly, some embodiments relate to a method for direct and quantitative, error-corrected counting of genomic sequences for determining immune response gene repertoires. | 2022-08-18 |
20220259660 | METHODS FOR THE ADMINISTRATION OF ILOPERIDONE - The present invention relates to methods for the identification of genetic polymorphisms that may be associated with a risk for QT prolongation after treatment with iloperidone and related methods of administering iloperidone to patients with such polymorphisms. | 2022-08-18 |
20220259661 | KIT FOR ASSESSING THE RISK OF COMPLICATIONS IN PATIENTS WITH SYSTEMIC INFLAMMATORY RESPONSE SYNDROME (SIRS) - A kit for in vitro measurement of a at least one IL7R gene transcript in a blood sample, including specific reagents for measuring the transcript, and a control sample calibrated to contain the IL7R gene transcript corresponding to the mean quantity measured in a pool of reference blood samples from human patients in a state of septic shock when reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and who were known to have survived, and/or a calibrated to contain the quantity of an IL7R gene transcript corresponding to the mean quantity measured in reference blood samples from patients in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and not to have survived. | 2022-08-18 |
20220259662 | BIOMARKER COMPOSITION FOR PREDICTING THERAPEUTIC EFFECT OF MESENCHYMAL STEM CELLS ON RENAL FIBROSIS - The present invention relates to a biomarker composition for predicting the therapeutic effect of mesenchymal stem cells on renal fibrosis. | 2022-08-18 |
20220259663 | METHOD FOR DIAGNOSING PROSTATIC DISEASE VIA BACTERIAL METAGENOMIC ANALYSIS - A method of diagnosing prostatic diseases such as prostate cancer, prostatic hyperplasia, and the like through bacterial metagenomic analysis, and more particularly, to a method of diagnosing prostate cancer or prostatic hyperplasia by analyzing an increase or decrease in content of extracellular vesicles derived from specific bacteria through bacterial metagenomic analysis using a subject-derived sample. An extracellular vesicle secreted from a bacterium present in the environment can be absorbed into the body and directly affect the occurrence of inflammation and cancer, and prostatic diseases such as prostate cancer, prostatic hyperplasia, and the like is difficult to diagnose early on before any symptom appears, which makes efficient treatment difficult. As such, through the metagenomic analysis on a gene present in a bacterium-derived extracellular vesicle using a human body-derived sample according to the present invention, the risk of the onset of prostate cancer and prostatic hyperplasia can be predicted in advance. | 2022-08-18 |
20220259664 | COMBINED AUXILIARY DIAGNOSTIC METHOD, KIT, SYSTEM AND USE OF POINT MUTATION AND METHYLATION OF BLADDER CANCER DRIVER GENES - Disclosed are the method, kit, system and use for screening or detecting or assisting in the diagnosis of bladder cancer, which are able to simultaneously detect FGFR3, TERT, PLEKHS1 point mutations and OTX1, NID2 and NRN1 methylation levels, thereby realizing the combined detection of gene point mutation and methylation, reducing the demand for samples, experimental operation steps and detection cycle, and improving the specificity and sensitivity of detection. | 2022-08-18 |
20220259665 | METHODS FOR PREDICTING THE SURVIVAL TIME AND TREATMENT RESPONSIVENESS OF A PATIENT SUFFERING FROM A SOLID CANCER WITH A SIGNATURE OF AT LEAST 7 GENES - The present invention relates to a method for predicting the survival time of a patient suffering from a solid cancer comprising i) determining in a tumor sample obtained from the patient the gene expression level of at least 7 genes selected from the group consisting of CCR2, CD3D, CD3E, CD3G, CD8A, CXCL10, CXCL11, GZMA, GZMB, GZMK, GZMM, IL15, IRF1, PRF1, STAT1, CD69, ICOS, CXCR3, STAT4, CCL2, and TBX21, ii) comparing every expression level determined at step i) with their predetermined reference value and iii) providing a good prognosis when all expression levels determined at step i) are higher than their predetermined reference values, or providing a bad prognosis when all expression levels determined at step i) are lower than their predetermined reference values or providing an intermediate prognosis when at least one expression level determined value is higher than its predetermined value. The method is also particularly suitable for predicting the responsiveness of the patient to a treatment. | 2022-08-18 |
20220259666 | ASSESSMENT OF THE P13K CELLULAR SIGNALING PATHWAY ACTIVITY USING MATHEMATICAL MODELLING OF TARGET GENE EXPRESSION - The present invention relates to a method comprising inferring activity of a PI3K cellular signaling pathway in a tissue and/or cells and/or a body fluid of a medical subject based at least on expression levels of one or more target gene(s) of the PI3K cellular signaling pathway measured in an extracted sample of the tissue and/or the cells and/or the body fluid of the medical subject. The present invention further relates to an apparatus comprising a digital processor configured to perform such a method, a non-transitory storage medium storing instructions that are executable by a digital processing device to perform such a method, and a computer program comprising program code means for causing a digital processing device to perform such a method. | 2022-08-18 |
20220259667 | SYSTEMS AND METHODS FOR CELL OF ORIGIN DETERMINATION FROM VARIANT CALLING DATA - The present invention relates generally to classification of biological samples, and more specifically to cell of original classification. In particular, some embodiments of the invention relate to diffuse large B cell lymphoma cell of origin classification using machine learning models. The machine learning models can be based on decision trees such as a random forest algorithm or a gradient boosted decision tree. Features for the models can be determined through analysis of variant data from plasma or blood samples from a plurality of subjects with the disease. | 2022-08-18 |
20220259668 | RAPID ANEUPLOIDY DETECTION - This document provides methods and materials for identifying chromosomal anomalies that can be used to identify a mammal as having a disease (e.g., cancer or congenital abnormality). For example, this document provides methods and materials for evaluating sequencing data to identify a mammal as having a disease associated with one or more chromosomal anomalies (e.g., cancer or congenital abnormalities). For example, this document provides methods and materials for evaluating sequencing data that can be used in cancer diagnostics, non-invasive prenatal testing (NIPT), preimplantation genetic diagnosis and evaluation of congenital abnormalities. | 2022-08-18 |