36th week of 2019 patent applcation highlights part 20 |
Patent application number | Title | Published |
20190270964 | CELL POPULATION COMPRISING MESENCHYMAL STEM CELLS DERIVED FROM FETAL APPENDAGE, METHOD FOR PRODUCING THE SAME, AND PHARMACEUTICAL COMPOSITION - Highly proliferative mesenchymal stem cells (MSCs) may be useful for the large-scale and rapid production of a cell preparation, and a cell population may include the mesenchymal stem cells. A cell population may include mesenchymal stem cells derived from a fetal appendage, wherein, in the cell population, the proportion of CD105 | 2019-09-05 |
20190270965 | METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO NEURAL PRECURSOR CELLS - The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small, molecule BMP inhibitor, and induced neural precursor cells prepared by this method. | 2019-09-05 |
20190270966 | Process of Expanding T Cells - The present disclosure relates to a novel process for expanding T cells, such as autologous T cells, cell populations therefrom, pharmaceutical compositions comprising the said cell populations and use of the cells and compositions for treatment, particular the treatment or prophylaxis of virus infection and/or cancer, for example in immune compromised or immune competent human patients. | 2019-09-05 |
20190270967 | IDENTIFICATION OF FACTOR THAT PROMOTES HUMAN HSC SELF-RENEWAL - Transient MLLT3 overexpression in culture may be used to expand human HSCs in vitro, and thereby improve the efficiency and safety of HSC transplantation. | 2019-09-05 |
20190270968 | METHODS AND PRODUCTS FOR TRANSFECTING CELLS - The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed. | 2019-09-05 |
20190270969 | METHOD TO SUPPRESS DEDIFFERENTIATION OF CELLS THAT READILY DEDIFFERENTIATE, METHOD FOR PREPARING SAID CELLS, AND METHOD FOR PRODUCING SUBSTANCE - The present invention relates to a method to suppress the dedifferentiation of cells that readily dedifferentiate, said method including: (1) a step in which the cells are applied to a polymer porous film; and (2) a step in which the cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids. | 2019-09-05 |
20190270970 | METHODS OF PRODUCING AND CHARACTERIZING VIRUS VACCINE AND VIRUS VACCINE COMPOSITION - This application pertains to methods of isolating virus particles and producing virus vaccine composition comprising subject a biological sample to an anion exchange chromatography and a hydroxyapatite chromatography. The application also pertains to rabies virus vaccine compositions and methods of assessing suitability of a virus vaccine composition or releasing a commercial batch of virus vaccine composition for clinical use. | 2019-09-05 |
20190270971 | INCREASING PRODUCTIVITY OF MICROBIAL HOST CELLS THAT FUNCTIONALLY EXPRESS P450 ENZYMES - The present technology relates to the production of chemical species in bacterial or yeast host cells. Particularly, the present technology provides for the production of chemical species in microbial host cells that functionally express engineered P450 enzymes. | 2019-09-05 |
20190270972 | METHODS FOR CONVERSION OF THE SUBSTRATE SPECIFICITY OF DESATURASES - The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity. | 2019-09-05 |
20190270973 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DP | 2019-09-05 |
20190270974 | POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME - The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates. | 2019-09-05 |
20190270975 | High Throughput Reaction Assembly - Provided herein is a reverse transcriptase mixture comprising a reverse transcriptase and a colored dye at a concentration in the range of 0.003%-1% (v/w). The colored dye may be visually observed during transfer of the mix from one vessel to another and addition of the mix to another mix can be confirmed by eye by observing the colored dye. | 2019-09-05 |
20190270976 | Method for Reducing Inhibitory Effect of Cyclodextrin on Pullulanase - The disclosure herein relates to a method for reducing the inhibitory effect of a cyclodextrin on a pullulanase and belongs to the technical field of gene engineering, enzyme engineering or food science. The method of the disclosure prepares a pullulanase mutant by reasonably mutating the key amino acid of the pullulanase interactive with the cyclodextrin to reduce the inhibitory effect of the cyclodextrin on the pullulanase, thereby improving the hydrolysis activity of the pullulanase. The disclosure finds the interactive sites of the pullulanase and the cyclodextrin based on analysis of crystal structures of enzymes and inhibitors and sequence comparison of enzymes from different sources, and utilizes site-directed mutation to obtain the pullulanase mutant having reduced sensibility to the cyclodextrin, thereby improving the utilization ratio of the starch raw material and the yield of the cyclodextrin. | 2019-09-05 |
20190270977 | NOVEL RECOMBINANT FACTOR C AND METHOD FOR PRODUCING THE SAME, AND METHOD FOR MEASURING ENDOTOXIN - A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from | 2019-09-05 |
20190270978 | A HIGHLY STABLE, PROTEASE-RESISTANT E. COLI ASPARAGINASE | 2019-09-05 |
20190270979 | NUCLEIC ACID EXTRACTION METHOD USING SOLID SUBJECT - The present invention relates to a nucleic acids extraction method using a solid subject, and it can isolate nucleic acids with large amounts and high purity at a low cost and simpler than conventional methods of extracting nucleic acids by forming a complex of a nucleic acids sample and an imidoesters compound in the subject, and particularly, the thin film device used for extracting nucleic acids has improved hydrophilicity compared to the conventional silicon substrate, so that nucleic acids can be extracted more efficiently. | 2019-09-05 |
20190270980 | TREATING CANCER - This document relates to methods and materials for treating cancer. For example, methods and materials for using CRISPR/Cas9 systems to treat cancer are provided. | 2019-09-05 |
20190270981 | POLYPEPTIDE LIBRARY - The invention relates to novel polypeptide libraries that are conformationally constrained in an anti-parallel, helix-turn-helix arrangement. The invention further relates to methods of generating and screening such libraries for biological, pharmaceutical and other uses. | 2019-09-05 |
20190270982 | METHOD FOR SCREENING APTAMER BY USING MICROARRAY MICROFLUIDIC CHIP - A method for screening aptamers by using a microarray microfluidic chip. The screening chip integrates microarray and microfluidic technology to integrate the positive and negative screening process on a microfluidic chip, and obtains aptamers with high affinity after 7 rounds of screening. At the same time, the present invention also discloses specific steps for screening of lactoferrin aptamers, including detailed processes such as chip preparation, positive and negative screening processes, and PCR amplification. The aptamers screened by the method have good specificity and affinity to the target protein. The aptamers are easier to be obtained than the antibody, and can be synthesized rapidly in large quantities in vitro. The preparation method is simpler and faster, so aptamers are expected to be a useful complement to antibody technology in many areas. | 2019-09-05 |
20190270983 | SINGLE CELL ANALYSIS OF TRANSPOSASE ACCESSIBLE CHROMATIN - Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions. | 2019-09-05 |
20190270984 | METHODS AND COMPOSITIONS FOR THE MAKING AND USING OF GUIDE NUCLEIC ACIDS - Provided herein are methods and compositions to make guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs from any source nucleic acid. Also provided herein are methods and compositions to use the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs in a variety of applications. | 2019-09-05 |
20190270985 | MONOLITHS WITH ATTACHED RECOGNITION COMPOUNDS, ARRAYS THEREOF AND USES THEREOF - Provided herein are monoliths with attached recognition compounds which selectively bind ligands, methods of preparing such monoliths, arrays thereof and uses thereof. For example, monoliths provided herein can be used in columns and arrays thereof. | 2019-09-05 |
20190270986 | COMPOSITIONS AND METHODS FOR ACTIVATING SILENT GENE CLUSTERS - The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor. | 2019-09-05 |
20190270987 | AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS - In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. | 2019-09-05 |
20190270988 | CRUDE BIOLOGICAL DERIVATIVES COMPETENT FOR NUCLEIC ACID DETECTION - The invention relates to methods for the detection of a specific sequence of RNA in a cell or tissue sample. The invention also relates to methods to enzymatically manipulate the RNA in a crude cell lysate in a number of applications. | 2019-09-05 |
20190270989 | Nonoparticles comprising protein-polynucleotide complexes and for delivering protein based complexes - This invention provides nanoparticles containing protein-polynucleotide complexes and methods of manufacture and methods of their use. These particles, when administered to a subject in need, are capable of delivering these complexes to target cells and target intracellular locations where they can perform a therapeutic function. In some embodiments, this therapeutic function includes gene editing, induction of gene skipping, and regulation of gene expression. The instant nanoparticles are generally formed by designing and synthesizing the polynucleotide to according to its intended function, combining it with a protein selected for its substrate specificity and enzymatic function in a manner to form a polynucleotide-protein complex, encapsulating the complexes by dispersion into a water-insoluble surfactant system, optionally adding a targeting ligand, and stabilizing the nanoparticles by crystallization of the ligand to the surface of the nanoparticles. | 2019-09-05 |
20190270990 | ANTISENSE COMPOUNDS AND USES THEREOF - The present disclosure provides oligomeric compounds. Certain such oligomeric compounds are useful for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell. In certain embodiments, hybridization results in modulation of the amount activity or expression of the target nucleic acid in a cell. | 2019-09-05 |
20190270991 | COMPOSITIONS FOR DELIVERY OF CARGO TO CELLS - The invention provides compositions for delivery of cargo to cells. The invention also provides compositions that bind multiple agents simultaneously. The compositions are useful as therapeutics. Methods of using the compositions are also provided. | 2019-09-05 |
20190270992 | METHODS OF CONTROLLING RED BLOOD CELL PRODUCTION - A method of controlling red blood cell production includes contacting red blood cell precursors with a composition including zinc, a composition including an inhibitor of a zinc exporter protein, or a combination thereof; wherein the contacting promotes survival of the red blood cell precursors, promotes terminal differentiation of the red blood cell precursors to mature red blood cells, or a combination thereof; or contacting red blood cell precursors with a composition including a zinc chelator, a composition including an inhibitor of a zinc importer protein, or a combination thereof; wherein the contacting inhibits survival of the red blood cell precursors, inhibits terminal differentiation of the red blood cell precursors to mature red blood cells, or a combination thereof. | 2019-09-05 |
20190270993 | Therapeutic Targeting of a microRNA to Treat Duchenne Muscular Dystrophy - Methods of treating Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), comprising administering an inhibitory nucleic acid that targets miR-128. | 2019-09-05 |
20190270994 | ANTISENSE MOLECULES AND METHODS FOR TREATING PATHOLOGIES - An antisense molecule capable of binding to a selected target site to induce exon skipping in the dystrophin gene, as set forth in SEQ ID NO: 1 to 59. | 2019-09-05 |
20190270995 | Methods and Compositions for Selecting siRNA of Improved Functionality - Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for TGF Beta 1. | 2019-09-05 |
20190270996 | CHIMERIC DOUBLE-STRANDED NUCLEIC ACID - A method of reducing the level of a transcription product in a cell comprising contacting with the cell a composition comprising a double-stranded nucleic acid complex comprising a first nucleic acid strand annealed to a second nucleic acid strand, wherein: (i) the first nucleic acid strand hybridizes to the transcription product and comprises (a) a region consisting of at least 4 consecutive nucleotides that are recognized by RNase H when the strand is hybridized to the transcription product, (b) one or more nucleotide analogs located on 5′ terminal side of the region, (c) one or more nucleotide analogs located on 3′ terminal side of the region and (d) a total number of nucleotides and nucleotide analogs ranging from 8 to 35 nucleotides and (ii) the second nucleic acid strand comprises (a) nucleotides and optionally nucleotide analogs and (b) at least 4 consecutive RNA nucleotides. | 2019-09-05 |
20190270997 | INHIBITION OF KMT2D FOR THE TREATMENT OF CANCER - The presently disclosed subject matter relates to the administration of a KMT2D inhibitor for the treatment of a cancer. The present invention is based on the discovery that upon PI3K inhibition, KMT2D activity is upregulated, resulting in an increase in the expression of genes involved in breast cancer cell proliferation and tumor growth. Accordingly, the present invention provides methods for treating a subject that has cancer by administering a therapeutically effective amount of an KMT2D inhibitor. | 2019-09-05 |
20190270998 | COMPOSITIONS AND METHODS COMPRISING PERMUTED PROTEIN TAGS FOR FACILITATING OVEREXPRESSION, SOLUBILITY, AND PURIFICATION OF TARGET PROTEINS - Provided are compositions and methods for used in solubilizing, stabilizing and expressing proteins. The proteins are fusion proteins that contain a protein of interest. The fusion proteins contain segments of Ribose Binding Protein (RBP) or Maltose Binding Protein (MBP). The fusion proteins can have the RBP or MBP segments flanking the target protein, and the RBP or MBP segments can be in the fusion protein in the same orientation as they normally occur (except for being interrupted by the target protein) or the segments can be permuted. Novel segments of the RBP and MBP are provided and result in improved expression and/or solubility of the proteins. Some examples include one or a combination of two complete or partial Histidine tags. Some examples allow for the target protein to be separated from all or a part of the fusion protein such as by enzymatic or non-enzymatic cleavage. | 2019-09-05 |
20190270999 | METHOD FOR MODULATING GENE EXPRESSION BY MODIFYING THE CPG CONTENT - The invention relates to nucleic acid modifications for a directed expression modulation by the targeted insertion or removal of CpG dinucleotides. The invention also relates to modified nucleic acids and expression vectors. | 2019-09-05 |
20190271000 | Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels with Desirable Leaf Quality - The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. | 2019-09-05 |
20190271001 | COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) ACTIVITY - Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast. | 2019-09-05 |
20190271002 | Rust Resistance Gene - The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens. | 2019-09-05 |
20190271003 | Chromobacterium Subtsugage Genes - Disclosed herein are the nucleotide sequences of the | 2019-09-05 |
20190271004 | COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA - Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is | 2019-09-05 |
20190271005 | GENOMIC ENGINEERING OF PLURIPOTENT CELLS - Provided are methods and compositions for obtaining genome-engineered iPSCs, and derivative cells with stable and functional genome editing at selected sites. Also provided are cell populations or clonal cell lines derived from genome-engineered iPSCs, which comprise targeted integration of one or more exogenous polynucleotides, and/or in/dels in one or more selected endogenous genes. | 2019-09-05 |
20190271006 | METHOD FOR PRODUCING TRANSGENIC CELL - A method for producing a transgenic cell, may include contacting, in vitro, (i) a virus comprising: a chimeric protein of an antibody-binding protein and a vesicular stomatitis virus G (VSV-G) protein; and a foreign gene, with (ii) a target cell and an antibody specific to the target cell, and/or a target cell comprising a membrane antibody, to infect the target cell with the virus. | 2019-09-05 |
20190271007 | METHOD FOR DIRECT TRANSFORMATION OF EXOGENOUS DNA INTO RESTING SPORES OF PENICILLIUM AMAGASAKIENSE - The present invention discloses a method for direct transformation of exogenous DNA into resting spores of | 2019-09-05 |
20190271008 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2019-09-05 |
20190271009 | METHODS, CELLS AND REAGENTS FOR PRODUCTION OF ISOPRENE, DERIVATIVES AND INTERMEDIATES THEREOF - This application describes methods, including non-naturally occurring methods, for biosynthesizing 3-hydroxy-3-methylglutaryl-coA and intermediates thereof, as well as non-naturally occurring hosts for producing 3-hydroxy-3-methylglutaryl-coA. This application also describes methods, including non-naturally occurring methods, for biosynthesizing isoprene and intermediates thereof, as well as non-naturally occurring hosts for producing isoprene. | 2019-09-05 |
20190271010 | SYNTHETIC BIOCHEMISTRY MOLECULAR PURGE VALVE MODULE THAT MAINTAIN CO-FACTOR BALANCE - The disclosure provides a metabolic pathway for producing a metabolite, the metabolic pathway having a co-factor purge valve system for recycling a cofactor used in the metabolic pathway. | 2019-09-05 |
20190271011 | PROCESSES FOR PRODUCING ETHANOL - The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention. | 2019-09-05 |
20190271012 | METHOD FOR PRODUCING A LONG CHAIN DICARBOXYLIC ACID BY FERMENTATION, FERMENTATION BROTH, TREATED FERMENTATION BROTH AND WASTEWATER - The present invention discloses a method for producing a long chain dicarboxylic acid by fermentation as well as a fermentation broth, a treated fermentation broth and a wastewater. The salt content in the fermentation broth is controlled to be below 20% by the fermentation method of the present invention. The method for producing a long chain dicarboxylic acid by fermentation provided by the present invention can effectively reduce the amount of alkali used in the fermentation process and the amount of acid used in the subsequent extraction of the long chain dicarboxylic acid, thereby reducing the amount of salt in the whole production process of the long chain dicarboxylic acid. At the same time, the method of the present invention also has many advantages such as shortening the fermentation time, increasing the acid production, reducing the amount of medium, and suitable for the production of various types of long-chain dicarboxylic acids, etc. Compared with the existing production process, the method of the present invention not only has significant cost advantages, but also can effectively reduce the pressure on resource and environment, thus it has a very obvious value advantage in industrialization. | 2019-09-05 |
20190271013 | REDUCING INSOLUBLE DEPOSIT FORMATION IN ETHANOL PRODUCTION - The present inventors have surprisingly discovered that phytic acid tenaciously precipitates with soluble metals in food or fuel ethanol-processing fluid, producing insoluble organometallic salt deposit or scale on the processing equipment that must be removed in order to facilitate further ethanol processing. The present invention relates to converting phytic acid salts or phytates to inorganic phosphates to improve metal solubility and reduce deposition within processing equipment. | 2019-09-05 |
20190271014 | Materials and Methods for Producing 6-Carbon Monomers - This document describes materials and methods for, for example, producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol. | 2019-09-05 |
20190271015 | ENZYMATIC SYNTHESIS OF KAVALACTONES AND FLAVOKAVAINS - Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers. | 2019-09-05 |
20190271016 | METHOD FOR THE SYNTHESIS OF TRYPTOPHAN ANALOGS IN AQUEOUS SOLVENTS AT REDUCED TEMPERATURES - The present disclosure provides methods for preparing tryptophans and tryptophan derivatives. The methods include: combining i) an indole substrate or azulene substrate, ii) a serine substrate, and iii) an engineered tryptophan synthase β-subunit (TrpB); and maintaining the resulting mixture under conditions sufficient to form the product compound. The engineered TrpB comprises a PLP binding loop mutation, a helix 1 mutation, a strand 7-8 mutation, or a combination thereof. New TrpB variants are also described. | 2019-09-05 |
20190271017 | METHOD FOR PREPARING FERMENTABLE SUGARS FROM LIGNOCELLULOSIC BIOMASS - A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided. | 2019-09-05 |
20190271018 | METHOD OF PRODUCING SACCHARIFIED SOLUTION FROM USED ABSORBENT ARTICLE - A simple method is provided for producing a saccharified solution from a used absorbent article such as a used diaper. In order to produce a saccharified solution from a used absorbent article comprising a non-cellulosic liquid permeable surface material and an absorbent body that contains pulp fibers, the used absorbent article is immersed, without first being decomposed, in a saccharifying enzyme solution containing cellulase. The pulp fibers in the used absorbent article are saccharified by the cellulase, producing a saccharified solution. The produced saccharified solution is exuded out of the used absorbent article through the liquid permeable surface material, so it is possible to easily separate and recover the saccharified solution from the used absorbent article while maintaining the outer shape. | 2019-09-05 |
20190271019 | CARBOHYDRATE-ENRICHED RECOMBINANT MICROORGANISMS - The present disclosure relates to recombinant microorganisms engineered for enhanced production of a desired carbohydrate, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present disclosure also provides related methods. | 2019-09-05 |
20190271020 | RHAMNOLIPID-PRODUCING CELL HAVING REDUCED GLUCOSE DEHYDROGENASE ACTIVITY - The invention relates to cells which make rhamnolipids and are genetically modified such that they have a decreased activity, compared to the wild type thereof, of a glucose dehydrogenase and to a method for producing rhamnolipids using the cells according to the invention. | 2019-09-05 |
20190271021 | RECOMBINANT POLYPEPTIDE PRODUCTION METHODS - Herein is reported a method for producing a fusion-polypeptide comprising the seeps of a) cultivating a mammalian cell comprising a nucleic acid encoding a variant fusion-polypeptide wherein the amino acid sequence of the fusion-polypeptide has been modified by replacing in a pro-fusion-polypeptide the endogenous protease cleavage site between the pro-peptide and the fusion-polypeptide with an exogenous (with respect to the origins of the parts of the fusion-polypeptide) or artificial protease cleavage site, and b) recovering the fusion-polypeptide or fusion-pro-polypeptide from the cell or the cultivation median and thereby producing the (recombinant) fusion-polypeptide. | 2019-09-05 |
20190271022 | Signal Polypeptide For Improved Secretion Of Protein - The invention relates to a signal polypeptide for improving excretory production of a heterologous polypeptide, proteins comprising the signal polypeptide, nucleic acids encoding the signal polypeptide, and methods of using thereof. | 2019-09-05 |
20190271023 | IMAGING CARTRIDGE, PIPETTE, AND METHOD OF USE FOR DIRECT SPUTUM SMEAR MICROSCOPY - An assembly for preparing a specimen is provided that may be configured to determine the presence of at least one microorganism specie in the specimen. The assembly may include a pipette configured to acquire a specimen from a sample and an imaging cartridge configured to be in fluid communication with the pipette. The imaging cartridge and the pipette may be configured to be irreversibly coupled such that the specimen is bio-contained within the imaging cartridge and the pipette when the imaging cartridge and pipette are coupled together. Associated methods of use are also provided. | 2019-09-05 |
20190271024 | METHODS FOR DISTINGUISHING AND IDENTIFYING PLANT VARIETIES - Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant. | 2019-09-05 |
20190271025 | METHOD TO DETECT PRE-SYMPTOM EARLY CANCER WITH ENHANCED ACCURACY - The current invention relates to the method to detect early stage pre-symptom cancer with enhanced accuracy. The claimed methods use anti-tumor agent to cause lysis of any existing cancer cells in a person, and causing release of genetic material from the lysed cancer cells into the blood stream of the person. The claimed methods further utilize a PCR method to amplify cancer gene in released generic material from a blood plasma sample of the person. The claimed methods further perform genetic sequencing on the blood plasma sample after PCR to identify existence of the cancer genes, thereby confirming existence of cancer. | 2019-09-05 |
20190271026 | COMPOSITIONS, METHODS, AND KITS FOR DETECTING AND IDENTIFYING MYCOBACTERIA - Provided herein are methods for detecting and identifying strains of mycobacteria, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided for the detection and differentiation of mycobacteria based on, for example, pathogenicity, species, and antibiotic resistance or sensitivity. Compositions and methods are provided herein to identify and differentiate mycobacteria in mixtures of different mycobacteria and mycobacteria and non-mycobacteria. | 2019-09-05 |
20190271027 | METHOD FOR DETECTING TARGET NUCLEIC ACID MOLECULE - A method for detecting a target nucleic acid molecule of the present invention includes a step of associating a first and third probes labeled with a first fluorescent substance which is an energy donor with a second probe labeled with a second fluorescent substance which is an energy acceptor to form an associate in a nucleic acid molecule; and a step of emitting light with an excitation wavelength of the first fluorescent substance to the associate to detect the target nucleic acid molecule using fluorescence released from the second fluorescent substance in the associate as an indicator, wherein a region associating with the second probe is between a region associating with the first probe and a region associating with the third probe in the target nucleic acid molecule. | 2019-09-05 |
20190271028 | METHODS FOR DETECTING TARGET NUCLEIC ACIDS IN A SAMPLE - The present invention provides probes, methods, kits, and apparatuses that provide accurate, rapid, and sensitive multiplexed detection, identification, and quantification of target nucleic acids in a sample. | 2019-09-05 |
20190271029 | Nucleic Acid Sequence and Capture by Formation of an Abasic Site-Derived Cross-Link - Disclosed herein is a method of covalently crosslinking DNA strands. In certain aspects, the method comprises incubating a hybridized, double-stranded DNA polynucleotide (dsDNA), comprising a probe strand that comprises an abasic (Ap) residue and an at least partially complementary target strand that comprises a 2′-deoxyadenosine (dA) residue, wherein incubation occurs under conditions that allow for a covalent crosslinking reaction to occur between the Ap residue in the probe strand and the dA residue in the target strand. | 2019-09-05 |
20190271030 | Spatially Encoded Biological Assays - The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature. | 2019-09-05 |
20190271031 | Spatially Encoded Biological Assays - The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature. | 2019-09-05 |
20190271032 | A METHOD FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES - The present invention relates generally to a method for the amplification of nucleic acid sequences, more specifically, to a method for the amplification and identification of target nucleic acid sequences using primers containing locked nucleic acids for tracing a product to its origin. | 2019-09-05 |
20190271033 | Amplification and Analysis of Whole Genome and Whole Transcriptome Libraries Generated by a DNA Polymerization Process - The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates. | 2019-09-05 |
20190271034 | PRIMER TECHNOLOGY - In a method of nucleic acid manipulation, (i) hybridizing a double stranded primer to the 3′ end of a single stranded nucleic acid template, wherein said primer comprises a double stranded region and a single stranded region, wherein the single stranded region is a 3′ overhang region and wherein the 3′ overhang region enables the double stranded primer to target and hybridize to the 3′ end of the nucleic acid template, wherein said single stranded 3′ overhang region comprises a degenerate sequence or a sequence comprising universal bases, and wherein the double stranded primer is made up of two separate strands; (ii) at least one round of polymerization using a polypeptide with 5′ to 3′ DNA polymerization activity, wherein at least the first round of polymerization comprises using a polypeptide with 5′ to 3′ DNA polymerization activity to carry out a primer extension reaction to synthesise nucleotides in a template dependent manner from the 3′ end of the single stranded region of said hybridized double stranded primer, wherein the hybridizing step (i) and at least the first primer extension reaction from the 3′ end of the single stranded region of said hybridized double stranded primer in step (ii) takes place without the formation of a phosphodiester bond between the 3′ end of the nucleic acid template and the double stranded primer of step (i). | 2019-09-05 |
20190271035 | Multiplex pyrophosphorolysis activated polymerization to amplify multiple almost-sequence-identical templates in a single reaction - Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer. | 2019-09-05 |
20190271036 | DYNAMIC FLUX NUCLEIC ACID SEQUENCE AMPLIFICATION - Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods. | 2019-09-05 |
20190271037 | DNA SEQUENCING USING CONTROLLED STRAND DISPLACEMENT - This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence. | 2019-09-05 |
20190271038 | METHODS FOR BIOLOGICAL SAMPLE PROCESSING AND ANALYSIS - Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte. | 2019-09-05 |
20190271039 | METHODS FOR BIOLOGICAL SAMPLE PROCESSING AND ANALYSIS - Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte. | 2019-09-05 |
20190271040 | METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING - Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands. | 2019-09-05 |
20190271041 | EPIGENETIC MODIFICATION OF MAMMALIAN GENOMES USING TARGETED ENDONUCLEASES - The present disclosure provides genetically engineered cell lines comprising chromosomally integrated synthetic sequences having predetermined epigenetic modifications, wherein a predetermined epigenetic modification is correlated with a known diagnosis, prognosis or level of sensitivity to a disease treatment. Also provided are kits comprising said epigenetically modified synthetic nucleic acids or cells comprising said epigenetically modified synthetic nucleic acids that can be used as reference standards for predicting responsiveness to therapeutic treatments, diagnosing diseases, or predicting disease prognosis. | 2019-09-05 |
20190271042 | IDENTIFICATION OF UNIQUE GENE EXPRESSION PROFILES IN CHILDREN WITH REGRESSIVE AUTISM SPECTRUM DISORDER (ASD) AND ILEOCOLITIS - The invention provides compositions and methods for identifying autism and autism spectrum disorders in humans. The invention also includes compositions and methods for identifying unique gene expression profiles in children with regressive autism spectrum disorder (ASD) and ileocolitis. | 2019-09-05 |
20190271043 | METHODS FOR SIMULTANEOUS AMPLIFICATION OF TARGET LOCI - The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons. | 2019-09-05 |
20190271044 | METHODS FOR SEQUENCING SAMPLES - Personalized medicine involves the use of a patient's molecular markers to guide treatment regimens for the patient. The scientific literature provides multiple examples of correlations between drug treatment efficacy and the presence or absence of molecular markers in a patient sample. Methods are provided herein that permit efficient dissemination of scientific findings regarding treatment efficacy and molecular markers found in patient tumors to health care providers. | 2019-09-05 |
20190271045 | SYSTEMS AND METHODS FOR DETERMINING A TREATMENT COURSE OF ACTION - The present disclosure relates to methods of determining a treatment course of action. In particular, the present disclosure relates to mutations in the gene encoding estrogen receptor and their association with responsiveness to estrogen therapies for cancer. | 2019-09-05 |
20190271046 | DETECTION METHOD - Provided herein are materials and methods for isolation of eukaryotic nucleic acid from a human or non-human animal stool sample. Also provided are methods of analysis of eukaryotic biomarkers present in a human or non-human animal stool sample. | 2019-09-05 |
20190271047 | PHOSPHATIDYLINOSITOL-3-KINASE PATHWAY BIOMARKERS - Methods for treating breast cancer, specifically cancers resistant to treatment with one or more known breast cancer treatment drugs, and related patient selection strategies for predicting patient response to drug therapy, such strategies including detecting the presence or absence in a patient of one or more of PIK3CA gene amplification, a mutation in PIK3CA, and a decrease in PTEN protein expression, and treating a patient positive for the presence of one or more of same by administering to the subject a pan-ErbB tyrosine kinase inhibitor. | 2019-09-05 |
20190271048 | PRIMER PAIR, KIT AND METHOD OF DETECTING BABESIA CANIS - Primer pair, kit and method of detecting | 2019-09-05 |
20190271049 | WATER PIPE COLLECTION BOX AND STAVE COOLER SUPPORT - A water pipe collection box and stave support for a cast copper stave cooler body panel that has disposed within it a circuit of water pipes with a number of loops each with an inlet end and an outlet end, and all such inlet ends and outlet ends clustered together in a single group that exits a backside of the copper stave cooler body panel. A cast copper stave cooler body panel that has disposed within a circuit of water pipes with a number of loops each with an inlet end and an outlet end, and all such inlet ends and outlet ends clustered together in a single group that exits a backside of the copper stave cooler body panel. A blast furnace having stave cooler body panels variously profiled to fit inside, and where each has disposed within it a circuit of water pipes with a number of loops each with an inlet end and an outlet end, and all such inlet ends and outlet ends are clustered together in a single group that exits a backside of each copper stave cooler body panel. | 2019-09-05 |
20190271050 | INDUCING COMPRESSIVE STRESS WITH SHOT PEEN ELEMENTS IN INTERNAL OPENING OF ADDITIVELY MANUFACTURED COMPONENT - The disclosure relates to inducing compressive stress with shot peen elements in an internal opening of an additively manufactured component. Methods according to the disclosure include: receiving a component made by a metal powder additive manufacturing process, the component including a body having an external surface and an internal opening passing at least partially through the body, the internal opening including an additively manufactured shot peen element detached from a surface of the internal opening, wherein the additively manufactured shot peen element is shaped to induce a residual compressive stress upon contact with the surface of the internal opening; and vibrating the component at a selected frequency, wherein the additively manufactured shot peen element induces the compressive stress against the surface of the internal opening during the vibrating. | 2019-09-05 |
20190271051 | HIGH-STRENGTH STEEL SHEET AND METHOD FOR PRODUCING SAME - To provide a high-strength steel sheet with excellent ductility and hole expansion formability, a yield ratio of less than 68%, and a tensile strength of 590 MPa or more, by having a predetermined chemical composition and a microstructure where ferrite is 35% or more and 80% or less and martensite is 5% or more and 25% or less in area ratio, retained austenite is 8% or more in volume fraction, the average grain size of ferrite, martensite and retained austenite is 6.0 μm or less, 3.0 μm or less and 3.0 μm or less respectively, the average aspect ratio of crystal grain of ferrite, martensite and retained austenite is each more than 2.0 and 15.0 or less, and the value obtained by dividing the Mn content (mass %) in retained austenite by the Mn content (mass %) in ferrite is 2.0 or more. | 2019-09-05 |
20190271052 | STEEL SHEET AND METHOD FOR PRODUCING THE SAME - Provided are a steel sheet which has a tensile strength of 900 MPa or higher and excellent weldability, and a method for producing the steel sheet. The steel sheet has a specific composition and a microstructure containing, in terms of area fraction, ferrite of 30% or less (including 0%), tempered martensite of 70% or more (including 100%), and the balance other than ferrite and the tempered martensite of 10% or less (including 0%) in total, the tempered martensite having an average grain size thereof is 5 μm or lower, the tempered martensite having iron-based carbides, which have average particle sizes of 100 nm or less, precipitated on grain boundaries, and the tempered martensite containing, in terms of atomic concentration, 5 atom % or more in total of Si and Mn on the grain boundaries of the tempered martensite. The steel sheet has a tensile strength of 900 MPa or higher. | 2019-09-05 |
20190271053 | STEEL STRIP FOR PRODUCING A NON-ORIENTED ELECTRICAL STEEL, AND METHOD FOR PRODUCING SUCH A STEEL STRIP - The invention relates to a steel strip for producing a non-oriented electrical steel. To achieve greatly improved frequency-independent magnetic properties, in particular greatly reduced hysteresis losses, in comparison with known electrical steels, the following alloy composition in wt % is proposed: C: ≤0.03, Al: 1 to 12, Si: 0.3 to 3.5, Mn: >0.25 to 10, Cu: >0.05 to 3.0, Ni: >0.01 to 5.0, total of N, S and P: at most 0.07, remainder iron and smelting-related impurities, with the optional addition of one or more elements from the group Cr, Mo, Zn and Sn, wherein the steel strip has an insulation layer substantially consisting of Al | 2019-09-05 |
20190271054 | METHOD OF PRODUCING GRAIN-ORIENTED ELECTRICAL STEEL SHEET - To provide a grain-oriented electrical steel sheet that has better magnetic property than conventional ones without requiring high-temperature slab heating, in the case of not performing intermediate annealing, the hot rolled steel sheet obtained by a predetermined step is subjected to hot band annealing, and, in a heating process in the hot band annealing, heating is performed at a heating rate of 10° C./s or less for 10 sec or more and 120 sec or less in a temperature range of 700° C. or more and 950° C. or less, and in the case of performing the intermediate annealing, in a heating process in final intermediate annealing, heating is performed at a heating rate of 10° C./s or less for 10 sec or more and 120 sec or less in a temperature range of 700° C. or more and 950° C. or less. | 2019-09-05 |
20190271055 | Coated Steel Strips, Coated Stamped Products and Methods - A pre-coated steel strip is provided. The steel strip includes a strip of base steel having a length, a width, a first side, and a second side. The length of the strip is at least 100 m and the width is at least 600 mm. An aluminum or an aluminum alloy pre-coating is on at least part of at least one of the first or second sides of the strip of base steel. A thickness t | 2019-09-05 |
20190271056 | Coated Steel Strips, Coated Stamped Products and Methods - A pre-coated steel strip is provided. The steel strip includes a strip of base steel having a length, a width, a first side, and a second side. The length of the strip is at least 100 m and the width is at least 600 mm. An aluminum or an aluminum alloy pre-coating is on at least part of at least one of the first or second sides of the strip of base steel. A thickness t | 2019-09-05 |
20190271057 | Coated Steel Strips, Coated Stamped Products and Methods - A pre-coated steel strip is provided. The steel strip includesa strip of base steel having a length, a width, a first side, and a second side. The length of the strip is at least 100 m and the width is at least 600 mm. An aluminum or an aluminum alloy pre-coating is on at least part of at least one of the first or second sides of the strip of base steel. A thickness t | 2019-09-05 |
20190271058 | Coated Steel Strips, Coated Stamped Products and Methods - A pre-coated steel strip is provided. The steel strip includes a strip of base steel having a length, a width, a first side, and a second side. The length of the strip is at least 100 m and the width is at least 600 mm. An aluminum or an aluminum alloy pre-coating is on at least part of at least one of the first or second sides of the strip of base steel. A thickness t | 2019-09-05 |
20190271059 | Laser Shock Peening Apparatus for Surface of Workpiece, and Laser Shock Peening Method - A laser shock peening apparatus for the surface of a workpiece, said apparatus comprising a resonant cavity. When said apparatus is used to conduct laser shock peening, because of the presence of the resonant cavity, shock waves that would typically escape outward may instead be utilized, and composite shock waves may be formed as a result of the wave reflection and convergence effects of the resonant cavity. Said waves can then be used on the surface of a workpiece twice or multiple times, thereby greatly increasing energy utilization rates. In addition, a fluid-based confinement layer is limited to the inside of the resonant cavity and has a fixed shape, thereby effectively solving the problems of the poor stability of a fluid-based confinement layer and the difficulty with controlling the thickness of such a confinement layer. | 2019-09-05 |
20190271060 | CORROSION RESISTANT ALUMINUM ALLOY - An aluminum alloy composition includes a corrosion resistant aluminum alloy selected from the group consisting of: a 1xxx series aluminum alloy, a 3xxx series aluminum alloy, and a 5xxx series aluminum alloy. The corrosion resistant aluminum alloy includes not greater than 0.04 wt. % Fe, not greater than 3 wt. % Mg, an effective amount of a corrosion resistant additive, and the balance being aluminum. | 2019-09-05 |
20190271061 | HIGH-STRENGTH MAGNESIUM ALLOY WHICH CAN RAPIDLY REACT WITH A MEDIUM AND A PRODUCTION PROCESS THEREOF - The present invention provides a high-strength magnesium alloy which can react rapidly with a medium and production processes thereof. In one embodiment, the magnesium alloy comprises gadolinium, yttrium, aluminum, zinc, zirconium, rhenium, silicon, copper, iron, nickel, gallium, indium, beryllium, calcium and magnesium. In another embodiment, the magnesium alloy comprises gadolinium, yttrium, aluminum, zinc, zirconium, rhenium, silicon, copper, iron, nickel, lanthanum, cerium, manganese, gallium, indium, beryllium, calcium and magnesium. | 2019-09-05 |
20190271062 | COMPOSITE SINTERED MATERIAL - A composite sintered material includes: a plurality of diamond grains having an average grain size of less than or equal to 10 μm; a plurality of cubic boron nitride grains having an average grain size of less than or equal to 2 μm; and a remainder of a binder phase, wherein at least parts of adjacent diamond grains are bound to one another, the binder phase includes cobalt, in the composite sintered material, a content of the diamond grains is more than or equal to 30 volume % and less than or equal to 94 volume %, a content of the cubic boron nitride grains is more than or equal to 3 volume % and less than or equal to 40 volume %, and a content of the cobalt is more than or equal to 3 volume % and less than or equal to 30 volume %. | 2019-09-05 |
20190271063 | STEEL SHEET FOR HOT STAMPING USE, METHOD OF PRODUCTION OF SAME, AND METHOD OF PRODUCTION OF HIGH STRENGTH PART - The present invention has as its object the provision of steel sheet for hot stamping use which is excellent in part strength after hot stamping and delayed fracture resistance comprised of large C content high strength steel sheet in which effective hydrogen traps are formed in the steel material. The steel sheet of the present invention solves this problem by forming Fe—Mn-based composite oxides in the steel sheet and trapping hydrogen at the interfaces of the composite oxides and matrix steel and in the voids around the composite oxides. Specifically, it provides steel sheet for hot stamping use which is comprised of chemical ingredients which contain, by mass %, C: 0.05 to 0.40%, Si: 0.02% or less, Mn: 0.1 to 3%, S: 0.02% or less, P: 0.03% or less, Al: 0.005% or less, Ti: 0.01% or less, N: 0.01% or less, one or both of Cr and Mo in a total of 0.005 to 1%, and O: 0.003 to 0.03% and which have a balance of Fe and unavoidable impurities and which contains average diameter 0.1 to 15 μm Fe—Mn-based composite oxide particles dispersed in the steel sheet or furthermore has crushed voids around the composite oxide particles, a method of production of the same, and a method of production of a hot stamped high strength part. | 2019-09-05 |