39th week of 2017 patent applcation highlights part 25 |
Patent application number | Title | Published |
20170275637 | NOVEL MAIZE UBIQUITIN PROMOTERS | 2017-09-28 |
20170275638 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING PLANT UTILITY - Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant. | 2017-09-28 |
20170275639 | PLANT-DERIVED ANTIBODIES AND DERIVATIVES THAT REDUCE RISK OF ANTIBODY-DEPENDENT ENHANCEMENT (ADE) OF INFECTION - The present invention describes the plant-based production of a therapeutic anti-virus antibody. | 2017-09-28 |
20170275640 | DOMINANT NEGATIVE MUTANT KRP PROTEIN PROTECTION OF ACTIVE CYCLIN-CDK COMPLEX INHIBITION BY WILD-TYPE KRP - Disclosed are mutant CDK inhibitor (CKI) polypeptides having dominant negative antagonist activity against wild-type CKI proteins, as well as related compositions, including nucleic acids and vectors encoding the mutant CKI polypeptides and transformed host cells and transgenic plants comprising such nucleic acids and vectors. Also disclosed are related methods for using the mutant proteins to modulate cell division in cells, particularly plant cells. | 2017-09-28 |
20170275641 | COMPOSITIONS AND METHODS FOR IMPROVING ABIOTIC STRESS TOLERANCE - The present invention relates to compositions and methods for improving yield, yield stability, and/or drought stress tolerance in plants. Plants and/or plant parts identified, selected and/or produced using compositions and methods of the present invention are also provided. | 2017-09-28 |
20170275642 | NUCLEOTIDE SEQUENCES ENCODING FASCIATED EAR3 (FEA3) AND METHODS OF USE THEREOF - Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds. | 2017-09-28 |
20170275643 | EXTENDING JUVENILITY IN GRASSES - The present invention relates to compositions and methods for modulating the juvenile to adult developmental growth transition in plants, such as grasses (e.g. maize). In particular, the invention provides methods for enhancing agronomic properties in plants by modulating expression of GRMZM2G362718, GRMZM2G096016, or homologs thereof. Modulation of expression of one or more additional genes which affect juvenile to adult developmental growth transition such as Glossy15 or Cg1, in conjunction with such modulation of expression is also contemplated. Nucleic acid constructs for down-regulation of GRMZM2G362718 and/or GRMZM2G096016 are also contemplated, as are transgenic plants and products produced there from, that demonstrate altered, such as extended juvenile growth, and display associated phenotypes such as enhanced yield, improved digestibility, and increased disease resistance. Plants described herein may be used, for example, as improved forage or feed crops or in biofuel production. | 2017-09-28 |
20170275644 | PLANTS WITH ENHANCED TOLERANCE TO MULTIPLE ABIOTIC STRESSES - The present disclosure describes genetically-modified plants having enhanced tolerance to multiple abiotic stressors, such as extreme temperatures (heat or cold) and/or drought. Abiotic stress tolerance is enhanced by ectopic expression of a heterologous glutaredoxin. Abiotic stress tolerance (particularly drought) is also enhanced by inhibited function, activity, or expression of an endogenous glutaredoxin. Methods of producing such genetically-modified plants are also disclosed. | 2017-09-28 |
20170275645 | HERBICIDE-TOLERANT PLANTS - The present invention provides herbicide-tolerant plants. The present invention also provides methods for controlling the growth of weeds by applying an herbicide to which herbicide-tolerant plants of the invention are tolerant. Plants of the invention may express an acetyl-Coenzyme A carboxylase enzyme that is tolerant to the action of acetyl-Coenzyme A carboxylase enzyme inhibitors. | 2017-09-28 |
20170275646 | Powdery Mildew Resistance Providing Genes in Cucumis Melo - The present invention relates to powdery mildew resistance providing genes of the | 2017-09-28 |
20170275647 | Fungal Resistant Plants Expressing ACD - The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae in plants and/or plant cells. This is achieved by increasing the expression of an ACD protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an ACD protein. | 2017-09-28 |
20170275648 | NOVEL CAS9 PROTEINS AND GUIDING FEATURES FOR DNA TARGETING AND GENOME EDITING - The present invention is directed to methods and compositions comprising novel CRISPR polypeptides and polynucleotides for site-specific cleavage and nicking of nucleic acids, transcriptional control and genome editing. | 2017-09-28 |
20170275649 | Muscle-Specific Nucleic Acid Regulatory Elements and Methods and Use Thereof - The present invention relates to nucleic acid regulatory elements that are able to enhance muscle-specific expression of genes, in particular expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly muscle-directed gene therapy, and for vaccination purposes. | 2017-09-28 |
20170275650 | ENDOSOMAL ESCAPE DOMAINS FOR DELIVERY OF MACROMOLECULES INTO CELLS - The disclosure provides fusion polypeptides and constructs useful for delivering diagnostics and therapeutics to cells. The fusion constructs include a protein transduction domain, a endosomal escape domain and a cargo domain. Also provided are methods of treating disease and disorders such as cell proliferative disorders. | 2017-09-28 |
20170275651 | PRODUCTION OF FATTY ACIDS AND DERIVATIVES THEREOF - Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods. | 2017-09-28 |
20170275652 | Host Cells and Methods for Production of Isobutanol - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 2017-09-28 |
20170275653 | ENZYME SCAFFOLDS AND METHODS OF USE - Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays. | 2017-09-28 |
20170275654 | ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL - A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 2017-09-28 |
20170275655 | CONVERSION OF BIOMASS TO USEFUL INTERMEDIATES - An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid. | 2017-09-28 |
20170275656 | GENETICALLY MODIFIED (R)-LACTIC ACID PRODUCING THERMOPHILIC BACTERIA - The invention relates to a genetically engineered thermophilic bacterial cell that is facultative anaerobic comprising: a) inactivation or deletion of the endogenous (S)-lactate dehydrogenase gene; b) introduction of a (R)-lactate dehydrogenase gene; c) inactivation or deletion of the endogenous pyruvate formate lyase A and/or B gene. | 2017-09-28 |
20170275657 | FERMENTATION ROUTE FOR THE PRODUCTION OF LEVULINIC ACID, LEVULINATE ESTERS AND VALEROLACTONE AND DERIVATIVES THEREOF - The invention provides processes for the conversion of pyruvate obtained from sugars or other carbon sources, to valuable C5 materials such as levulinic acid, levulinate esters, valerolactone, and derivatives thereof. | 2017-09-28 |
20170275658 | METHOD OF MANUFACTURING AN AMINO-ACID COMPOSITION USING ANIMAL BY-PRODUCTS - The present disclosure provides a method for preparing an amino acid composition using an animal byproduct, particularly a method for obtaining an amino acid composition of high quality using an animal byproduct more effectively in short time. Because an amino acid composition can be obtained from an animal byproduct more effectively and quickly using the method of the present disclosure, utilization of livestock waste, etc. can be enhanced and application as various products can be expected. | 2017-09-28 |
20170275659 | RECOMBINANT CELLS AND METHODS FOR BIOSYNTHESIS OF ENT-ATISERENOIC ACID - This disclosure describes recombinant cells and methods for microbial biosynthesis of ent-atiserenoic acid. Thus, in one aspect, this disclosure describes a recombinant cell genetically modified to exhibit increased biosynthesis of ent-atiserenoic acid compared to a comparable control cell. In some cases, the recombinant cell can include a host cell modified to include at least one heterologous polynucleotide encoding at least one enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. In some cases, the recombinant cell can include a host cell and at least one heterologous enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. | 2017-09-28 |
20170275660 | PURINE ALKALOID-PRODUCING MICROORGANISMS AND METHODS OF MAKING AND USING THE SAME - Provided herein are microorganisms that include one or more heterologous nucleic acid selected from the group of a sequence encoding a 7-methylxanthosine synthase, a sequence encoding a theobromine synthase; and a sequence encoding a caffeine synthase, where the microorganism is capable of producing one or more purine alkaloid in a culture medium, when the microorganism is cultured under conditions sufficient to produce the one or more purine alkaloid. Also provided compositions and kits that include at least one of these microorganisms, and methods of producing one or more purine alkaloid that include culturing one of these microorganisms under conditions sufficient to produce the one or more purine alkaloid. | 2017-09-28 |
20170275661 | PROCESS FOR THE PRODUCTION OF ISOMALTOOLIGOSACCHARIDES - The present invention provides a method for the production of oligosaccharides by the fermentation of dextran-sucrase-producing microorganisms with sucrose and maltose. The disclosed process allows for the control of the final composition of the isomaltooligosaccharides by adjustments to pH and the initial ratio of sucrose to maltose. | 2017-09-28 |
20170275662 | Method and Apparatus for Controlled Hydrolysis - A method and apparatus for controlled hydrolysis. The method can comprise hydrolyzing a first reagent in a first hydrolysis reaction and deactivating a first enzyme catalyzing the first hydrolysis reaction. The deactivating step can occur in about 10 seconds or less; the deactivating step can comprise adding a deactivating fluid to a composition comprising the first enzyme and heating the first enzyme using a deactivating mechanism. In other aspects, hydrolyzing the first reagent and deactivating the first enzyme can occur in a conduit, and the first hydrolysis reaction can occur in a composition that is at least 50% water by weight. The apparatus can provide a hydrolysis reactor comprising: a conduit; a composition inlet in the conduit for a composition; a first enzyme inlet in the conduit downstream of the composition inlet; and a first deactivating mechanism downstream of the first enzyme inlet to deactivate the first enzyme. | 2017-09-28 |
20170275663 | METHOD OF PRODUCING SUGAR LIQUID - A method of producing a sugar liquid includes: (A) reacting mannanase with a liquid component obtained by hydrolysis treatment of woody biomass to obtain a saccharified liquid; and (B) filtering the saccharified liquid in Step (A) through a microfiltration membrane and/or ultrafiltration membrane to collect a sugar liquid from a permeate side. | 2017-09-28 |
20170275664 | Milling Process - The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of a beta-xylosidase, wherein step c) is performed before, during or after step b). | 2017-09-28 |
20170275665 | DIRECT CRISPR SPACER ACQUISITION FROM RNA BY A REVERSE-TRANSCRIPTASE-CAS1 FUSION PROTEIN - The present disclosure provides methods and compositions for the integration of a target RNA or DNA into a DNA substrate. Also provided are methods of forming RNA-DNA bonds and enzymes for performing the same. | 2017-09-28 |
20170275666 | Methods for Preparing Rebaudioside I and Uses - Methods of preparing rebaudioside I, including highly purified rebaudioside I, are described herein. The methods utilize biocatalysts for converting rebaudioside A to rebaudioside I. Compositions and consumables comprising rebaudioside I, including sweetener compositions and flavor enhancing composition, are also provided. | 2017-09-28 |
20170275667 | METHOD FOR QUANTIFYING AT LEAST ONE MICROORGANISM GROUP VIA MASS SPECTROMETRY - A method for quantifying at least one microorganism group via at least one mass spectrometry analysis. The method includes at least one separation and fragmentation step. The method moreover includes a step that involves measuring the amount of at least one representative peptide or at least one protein representing the microorganism group. The at least one representative peptide or the at least one protein is obtained after the at least one separation and fragmentation step and serves as a quantification marker(s). The amount of the quantification marker(s) is directly correlatable to the amount of the at least one microorganism group. | 2017-09-28 |
20170275668 | METHODS FOR DETECTING AND MEASURING POLYSACCHARIDE-HYDROLYZING ENZYMES - Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration. | 2017-09-28 |
20170275669 | METHODS, KITS, AND SYSTEMS FOR MULTIPLEXED DETECTION OF TARGET MOLECULES AND USES THEREOF - Described herein are methods, compositions, kits and systems for multiplexed detection of target molecules from a sample. In some embodiments, the methods, compositions, kits and systems can be used to perform multiplexed protein analysis of a sample (e.g., a sample comprising a small number of cells or a single-cell sample). In some embodiments, the same sample subjected to a multiplexed protein analysis using the methods, compositions, kits and systems described herein can also be subjected to a nucleic acid (e.g., RNAs, microRNAs, and/or DNA) analysis, thereby creating an integrated expression profiling from a limited amount of sample. | 2017-09-28 |
20170275670 | METHOD AND APPARATUS FOR ENRICHING PATHOGEN DNA - A method and an apparatus are provided for enriching pathogen DNA. The method includes adding a selective lysis buffer to a sample, incubating the mixture formed thereby, and filtering the mixture. The apparatus for enriching pathogen DNA includes: a lysis chamber; a reservoir containing selective lysis buffer, connected to the lysis chamber; and a filter connected to the lysis chamber; that achieves a limit of detection of pathogens of 500 cfu/ml or less. | 2017-09-28 |
20170275671 | METHODS AND SOLUTIONS FOR INHIBITING UNDESIRED CLEAVING OF LABELS - The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. | 2017-09-28 |
20170275672 | CHEMICALLY MODIFIED LIGASE COFACTORS, DONORS AND ACCEPTORS - Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process. | 2017-09-28 |
20170275673 | METHODS AND COMPOSITIONS RELATED TO PROSTATE CANCER THERAPEUTICS - Disclosed herein are compositions and methods for detecting biological molecules and biomarkers associated with prostate cancer. Disclosed herein are compositions and methods for detecting biological molecules and biomarkers associated with castration-resistant prostate cancer wherein such biological molecules and biomarkers comprise androgen-receptor splice variants that can be used to develop effective therapeutic regimens for prostate cancer patients. Disclosed herein are methods of using biological molecules and biomarkers related to androgen-receptor splice variants for assessing therapeutic resistance to drugs such as enzalutamide and abiraterone. | 2017-09-28 |
20170275674 | NEXT-GENERATION SEQUENCING QUALITY CONTROL MONITORING TOOL - Systems and methods are used to provide an online tool for clinical laboratories to monitor Next-Generation Sequencing (NGS) workflow using Quality Control (QC) material that can be used for multiple assays. The tool utilizes a highly multiplexed QC with NGS assays that detect somatic mutations. The control provides a common QC material that can be used across laboratories with different NGS instrument platforms, assays and bioinformatics pipelines to test precision and detect analytical deviations that may arise from reagent and instrument variation. | 2017-09-28 |
20170275675 | DETECTION METHOD AND KIT OF BASE MUTATION, AND METHOD FOR LIMITING PCR AMPLIFICATION OF NUCLEIC ACID SAMPLE - Detection method of a base mutation in a target base sequence of a nucleic acid sample, includes: performing a PCR reaction with the nucleic acid sample as a template, using a primer set capable of amplifying, by PCR, an amplification target region including the target base sequence; a blocker nucleic acid fragment having a base sequence complementary to the target base sequence and including a residue which is synthetic nucleic acid; and a probe that hybridizes to a region, in the target region, closer to a 5′ end of the target region than the target base sequence in the same chain as the target base sequence, and that has a fluorescent substance on one of a 5′ end and a 3′ end of the probe and a quenching substance on the other; and measuring an amplification amount of the template in the PCR reaction by detecting fluorescence from the probe. | 2017-09-28 |
20170275676 | METHODS FOR ANALYZING NUCLEIC ACID - The invention generally relates to methods for analyzing nucleic acids. In certain aspects, methods of the invention involve obtaining a sample including a nucleic acid template. A plurality of molecular inversion probes are tiled across a portion of the template. The probes are designed such that immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner. A region between targeting arms of a plurality of the molecular inversion probes is filled-in with nucleotides, and the filled-in region of a plurality of the probes is analyzed to obtain sequence information about the nucleic acid template. | 2017-09-28 |
20170275677 | Time-Resolved Nucleic Acid Hybridization Beacons - Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations. | 2017-09-28 |
20170275678 | METHODS OF DETECTING TARGET NUCLEIC ACIDS - The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore. | 2017-09-28 |
20170275679 | USE OF GEL BEADS TO CONTROL DROPLET DISPERSION - A method of generating deformable gel beads that contain a single colloidal particle is described. Gel beads containing a particle are isolated from those that do not contain a particle, based on differences between the buoyant density of these gel bead populations. The gel beads containing a particle are subsequently co-encapsulated at a high efficiency into droplets that can comprise additional objects such as cells, other particles, or reagents. The gel coating on the rigid particle prevents clogging in narrow channels, such as those of a droplet generator. | 2017-09-28 |
20170275680 | Methods and Apparatus for Nanoparticle-assisted Nucleic Acid Amplification, Hybridization and Microarray Analysis - Nucleic acid hybridization methods are disclosed. An example method comprises: immobilizing probe nucleic acid molecules on a surface; flowing target nucleic acid molecules to the immobilized probe nucleic acid molecules on said surface in a hybridization buffer solution; washing said surface with a wash solution which comprises nanoparticles; and detecting the presence of duplexes on said surface comprising a strand of one of said target nucleic acid molecules and a strand of one of said probe nucleic acid molecules. In some embodiments, the target nucleic acid molecules are generated using a helicase-dependent amplification method wherein the reaction solution comprises nanoparticles. | 2017-09-28 |
20170275681 | SINGLE MOLECULE RNA DETECTION - Quantification of RNAs is one of the most essential tools to characterize cells. This tool is widely used in disease diagnosis, pharmacogenomics, and drug development. Single cell RNA fluorescent in situ hybridization (smRNA-FISH) revolutionized RNA detection and quantification by detecting every single RNA molecule of a gene. However, this technology is incapable of assaying relatively short RNAs, and it suffers from high cost and low throughput. Here, we describe a technology that simultaneously overcomes the three drawbacks using conventional instrumentation. This QD-smRNA-FISH technology uses hybridization of quantum dot-labeled DNA oligonucleotides to the RNA molecules for visualization and counting. Quantum dots (QDs) have been assumed inapplicable to counting individual RNA molecules, due to the well-known blinking problem (display intermittency) (Medintz I L, Uyeda H T, Goldman E R, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4: 435-446). This problem has been circumvented by this new experimental design. In some embodiments described herein, the methods assemble several QDs to every target RNA molecule and leverage the complementation of the QDs to achieve an overall non-intermittent signal on each target molecule. We validated QD-smRNA-FISH by comparing its signals with those of standard smRNA-FISH. We successfully applied QD-smRNA-FISH to test the interaction of two RNAs, a task that cannot be accomplished with standard smRNA-FISH. The QD-smRNA-FISH method offers a highly accurate method for single RNA molecule detection and counting under standard fluorescent microscopes, and enables analysis of relatively short RNAs (<1000 bases) which comprises the majority of eukaryotic transcriptome and more than half of the eukaryotic mRNAs. The QD-smRNA-FISH method also reduces the reagent cost by several folds and allows for analysis of multiple genes in parallel. | 2017-09-28 |
20170275682 | NUCLEIC ACID AMPLIFICATION REACTION METHOD, NUCLEIC ACID AMPLIFICATION REACTION APPARATUS, AND NUCLEIC ACID AMPLIFICATION REACTION REAGENT - A nucleic acid amplification reaction method includes subjecting a reaction mixture containing a nucleic acid amplification reaction reagent to be used for amplifying a nucleic acid to a thermal cycle for amplifying the nucleic acid, wherein in the thermal cycle, a heating time for an annealing reaction and an elongation reaction is 1 sec or more and 10 sec or less, the nucleic acid amplification reaction reagent contains a forward primer, a reverse primer, a polymerase, and a fluorescently labeled probe, the concentration of the forward primer is 0.4 μM or more and 3.2 μM or less, the concentration of the reverse primer is 0.4 μM or more and 3.2 μM or less, the amount of the polymerase is 0.5 U or more and 4 U or less, and the concentration of the fluorescently labeled probe is 0.15 μM or more and 1.2 μM or less. | 2017-09-28 |
20170275683 | Method for High-Throughput, Ultra Long-Read DNA Sequencing - The Invention is a method for ascertaining extremely long DNA sequence reads (kilobases or megabases) from polony-type DNA sequencers. Polony-type DNA sequencers (e.g., Illumina, Roche, and Life Technologies sequencers) typically give read lengths of only about 500 bp. The Invention can extend those read lengths by orders of magnitude. | 2017-09-28 |
20170275684 | METHODS OF DETECTING NUCLEIC ACIDS AND APPLICATIONS THEREOF - The present application provides simple, specific and sensitive methods for detecting a target nucleic acid in a biological sample, detecting a pathogen in a biological sample, and diagnosing a disease in an individual, utilizing a combination of nucleic acid hybridization-based capture and ligation-enabled PCR. The methods are particularly useful for detecting low level nucleic acids and pathogens and for automation and processing of multiple biological samples. | 2017-09-28 |
20170275685 | METHOD FOR NUCLEIC ACID AMPLIFICATION - It is an object of the present invention to provide a method for amplifying a nucleic acid, using RNA as a template, which can realize elimination of the risk of non-specific amplification caused by DNA mixed from reagents and/or working environment, an increase in the detection sensitivity of trace RNA, and a reduction in amplification bias. According to the present invention, there is provided a method for amplifying a nucleic acid, which comprises a step of incubating a mixture comprising template RNA, a primer, a degrading enzyme specific to DNA in RNA-DNA hybrid, an RNase H minus reverse transcriptase, and a substrate. | 2017-09-28 |
20170275686 | METHODS FOR DETECTING GENOMIC DNA METHYLATION - The presently disclosed subject matter provides high-throughput methods for performing genomic DNA methylation assessments. The presently disclosed subject matter further provides methods for diagnosing a subject with a disease and/or disorder, and for determining the prognosis of a subject that has a disease and/or disorder. In certain embodiments, the present disclosure provides a diagnostic method that includes obtaining a biological sample from the subject; determining the methylation status of one or more genomic DNA loci in one or more cells of the biological sample; and diagnosing a disease and/or disorder in the subject, wherein the methylation status of the one or more genomic DNA loci indicates the presence of the disease and/or disorder in the subject. | 2017-09-28 |
20170275687 | NUCLEIC ACID SEQUENCING BY ELECTROCHEMICAL DETECTION - Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to devices, methods, and systems for sequencing-by-synthesis using changes in pH to monitor base addition. | 2017-09-28 |
20170275688 | METHODS, COMPOSITIONS AND SYSTEMS FOR SAMPLE DEPOSITION - Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles. | 2017-09-28 |
20170275689 | Combinatorial DNA Screening - The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome. | 2017-09-28 |
20170275690 | PHOTONIC SUPERLATTICE-BASED DEVICES AND COMPOSITIONS FOR USE IN LUMINESCENT IMAGING, AND METHODS OF USING THE SAME - Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined though at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice. The device further can include a second material having a second refractive index that is different than the first refractive index. The second material can be disposed within, between, or over the first and second pluralities of features and can include first and second luminophores. The device further can include a first optical component disposed over one of the first and second major surfaces of the first material. The first optical component can receive luminescence emitted by the first luminophore at the first wavelength approximately at the first angle, and can receive luminescence emitted by the second luminophore at the second wavelength approximately at the first angle. | 2017-09-28 |
20170275691 | SYNTHETIC NUCLEIC ACID SPIKE-INS - This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays. | 2017-09-28 |
20170275692 | Methods of Genomic Evaluation in Livestock - The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning. | 2017-09-28 |
20170275693 | NUCLEIC ACID ANALYSIS USING EMULSION PCR - The present invention provides methods for analyzing large nucleic acids including chromosomes and chromosomal fragments. In one aspect, the present invention provides a method of nucleic acid analysis comprising the steps of (a) obtaining a sample of nucleic acid comprising at least one chromosome or fragment greater than about 1 000 base pairs in length and containing a target region; (b) creating an emulsion in which each drop of the emulsion contains an average of between about 0-2, 0-1.75, 0-1.5, 0-1.0, 0-0.75, 0-0.5, or fewer chromosomes or fragments of step (a), (c) performing emulsion PCR, (d) quantifying the number of emulsion droplets containing amplified nucleic acid from the target region; (e) calculating the ratio of droplets containing amplified nucleic acid from the target region to total droplets; and (f) comparing the ratio of step (e) to a reference ratio representing a known genotype. | 2017-09-28 |
20170275694 | SESTRIN ACTIVATORS FOR PREVENTING AND/OR ATTENUATING SKIN AGEING AND/OR HYDRATING THE SKIN AND/OR FOR REGULATING SKIN PIGMENTATION - The disclosure relates to the identification and the use of compounds which regulate the expression of at least one Sestrin gene, for preventing and/or attenuating skin ageing, and/or for hydrating the skin and/or for regulating skin pigmentation. The method includes the following steps: a. bringing at least one test compound in contact with a sample of human keratinocytes or melanocytes; b. measuring the expression of at least one Sestrin gene chosen from SESN3, SESN2 and SESN1 in the keratinocytes or melanocytes; c. selecting the compounds for which an activation of at least 1.6 fold of the expression of at least one of the genes is measured in the keratinocytes treated in a. compared with the untreated keratinocytes, or for which a significant modulation of the expression of at least one of the genes is measured in the melanocytes treated in a. compared with the untreated melanocytes. | 2017-09-28 |
20170275695 | METHODS AND SYSTEMS FOR MONITORING, DIAGNOSING, AND TREATING CHRONIC OBSTRUCTIVE PULMONARY DISEASE - A novel set of 98 genes expressed in the respiratory tract epithelium that serve as biomarkers for measuring chronic obstructive pulmonary disease (COPD) activity are provided. Methods of classifying the (COPD) status of a subject are provided. Systems for expression-based classification of COPD disease status are provided. Methods of treating COPD are also provided, among other things. | 2017-09-28 |
20170275696 | CIRCULATING TUMOUR CELL TYPING AND IDENTIFICATION KIT - This disclosure relates to a circulating tumour cell typing and identification kit, comprising a capture probe, an amplification probe, and a labeled probe for each marker gene mRNA, wherein the marker gene mRNA comprises the following two types: at least two epithelial cell marker gene mRNAs selected from the group consisting of EPCAM, E-cadherin, CEA, KRT5, KRT7, KRT17, and KRT20 mRNAs; and, at least two mesenchymal cell marker gene mRNAs selected from the group consisting of VIMENTIN, N-cadherin, TWIST1, AKT2, ZEB2, ZEB1, FOXC1, FOXC2, SNAI1 and SNAI2 mRNAs. This disclosure prevents false-positive results caused by, for example, possible presence of a number of non-neoplastic epithelial cells in peripheral blood, introduction of normal epithelial cells during blood sampling, and the like. Accordingly, it may be assured that cells detected with epithelial cell marker genes and/or mesenchymal cell marker genes are indeed circulating tumour cells, further improving accuracy and reliability of the detection results. | 2017-09-28 |
20170275697 | Triaging of Patients Having Asymptomatic Hematuria Using Genotypic and Phenotypic Biomarkers - New methods for identifying patents with hematuria who are at low risk of having urothelial cancer (UC) include combining selected phenotypic variables with levels of genotypic expression into a new metric, the “G+P INDEX.” The G+P INDEX combines age, sex, smoking history, presence of hematuria, and frequency of hematuria with genotypic expression of the genetic markers, MDK, CDC2, HOXA13, IGFBP5, and optionally IL8Rb, then determining of the G+P INDEX value obtained for a patient is within one of three groups, either: (1) at High Risk of UC, (2) at Risk of UC, or (3) at Low Risk of UC. For groups 1 and 2, further clinical and laboratory work up is indicated, and patients in group 3 are monitored periodically to determine the need for further clinical workup. Using the G+P INDEX can save substantial time, effort, and funds by avoiding unnecessary medical diagnostic procedures for patients at Low Risk of UC. | 2017-09-28 |
20170275698 | METHOD FOR DETERMINING WHETHER OR NOT AQUEOUS SOLUTION CONTAINS TWO OR MORE CANCER CELLS - The present invention provides a method for determining whether or not an aqueous solution contains two or more cancer cells. The present method is characterized by the following three matters. First, the PCR solution contains the TS primer at a concentration of not less than 0.1 μM and not more than 1 μM in the present invention. Second, the PCR solution contains an ACX reverse primer. Third, the PCR solution contains the ACX reverse primer at a concentration of not less than 0.02 μM and not more than 0.06 μM in the present invention. In the present method, it is determined that an aqueous solution contains cancer cells even if the aqueous solution contains only two cancer cells. | 2017-09-28 |
20170275699 | PANCREATIC CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD - This invention provides a kit or a device for the detection of pancreatic cancer, comprising a nucleic acid(s) capable of specifically binding to a miRNA(s) in a sample from a subject, and a method for detecting pancreatic cancer, comprising measuring the miRNA(s) in vitro. | 2017-09-28 |
20170275700 | METHODS AND MATERIALS FOR IDENTIFYING METASTATIC MALIGNANT SKIN LESIONS AND TREATING SKIN CANCER - This document provides methods and materials for identifying metastatic malignant skin lesions (e.g., malignant pigmented skin lesions). For example, methods and materials for using quantitative PCR results and correction protocols to reduce the impact of basal keratinocyte contamination on the analysis of test sample results to identify metastatic malignant skin lesions are provided. This document also provides methods and materials for treating skin cancer. For example, methods and materials for identifying a mammal (e.g., a human) having a pre-metastatic skin lesion (e.g., pre-metastatic melanoma) and treating that mammal with pentamidine (4,4′-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide) are provided. | 2017-09-28 |
20170275701 | SYSTEMS AND METHODS FOR CHARACTERIZING CANCER - A method of identifying gene expression associated with recurrence free survival in a subject with cancer, comprising: a) assaying a sample from a subject diagnosed with cancer for the presence of altered gene expression of one or more genes selected from the group consisting of ADK, AP2B1, AVL9, CANX, DBT, DHRS7, DONSON, FAM190B, FGFR1, FOXN3, FZD5, GGH, GM2A, IGFBP5, ITSN2, LAMC1, LIFR, METTL7A, MT1F, MT1G, MT1P2, MT1X MT2A, NAB1, NCOA1, NCOR1, PAPOLA, PPME1, PPP1R13L, PRKAR2A, RABEP1, RBBP8, SGPL1, SIRT1, SNX2, SREK1, TAF1B, TMED5, and ZMIZ2; and b) identifying an outcome of decreased likelihood of recurrence free survival when altered gene expression relative to the level in a non-cancerous sample is present. | 2017-09-28 |
20170275702 | METHOD OF ANALYSIS ALLOWING AVOIDANCE OF SURGERY - A multi-gene-based assay for analysis of the following: (a) oncogene expression; (b) DNA methylation of tumor suppressor genes; (c) non-coding RNA expression (microRNA profiling); and (d) long non-coding RNA expression in cancer samples is disclosed. The assay method and device for conducting the assay are applicable to diagnosis, prognosis, and treatment of various cancers such as lung, breast, colorectal, prostate, liver, bladder; kidney, cervix, pancreatic, gastric, brain, oral, endometrium, and ovary. The assay methods allow avoidance of surgery to remove cancerous tissue. | 2017-09-28 |
20170275703 | TREATMENT AND DETECTION OF MELANOMA - Provided are methods for determining or predicting the diagnosis, prognosis, treatment and therapeutic efficacy of melanoma in a subject, which include evaluating the expression level of one or more miRNA biomarkers including miRNA-4487, miRNA-4706, miRNA-4731 and fragments or variants thereof as an indication of whether the subject may have, or be predisposed to, melanoma. | 2017-09-28 |
20170275704 | METHODS OF DETECTING PROSTATE CANCER - The invention described herein provides biological markers for the diagnosis, prognosis, and monitoring of prostate cancer | 2017-09-28 |
20170275705 | BIOMARKERS USEFUL FOR DETERMINING RESPONSE TO PD-1 BLOCKADE THERAPY - PD-L1 expression by tumor cells prior to treatment correlates highly with response to anti-PD-1 and anti-PD-L1 therapy (e.g., nivolumab (Bristol-Myers Squibb), pembrolizumab (Merck)) and anti-PD-L1 monotherapy (MPDL3280A (Genentech/Roche)). Nonetheless, the majority of patients with PD-LI(+) tumors do not respond to PD-1 pathway blockade. Distinct gene profiles associated with differential response to treatment with an anti-PD-1 antibody in patients with PD-L1+ renal cell carcinoma have been identified. In particular, a strong up-regulation of genes involved in metabolic functions and pathways was found in patients not responding to the therapy. Additionally, a down-regulation of genes involved in cellular migration functions was found in the same group of patients (non-responders). Specific biomarkers can be used to stratify responders from non-responders for PD-1 pathway blocking drugs. Additionally, the biomarkers represent therapeutic targets for anti-PD-1 combination therapy, and companion diagnostic products for such combination therapies. | 2017-09-28 |
20170275706 | ALK AND ROS KINASE IN CANCER - The invention provides a method for identifying a patient with cancer or suspected of having cancer as a patient likely to respond to an ALK- and/or ROS-inhibiting therapeutic, comprising: contacting a biological sample from a patient with a first reagent that specifically binds a polypeptide having ROS kinase activity and a second reagent that specifically binds to a polypeptide having ALK knase activity, and detecting whether the first reagent or the second reagent specifically binds to the biological sample, wherein detection of binding of either the first reagent or the second reagent to the biological sample identifies the patient as a patient likely to respond to an ALK-inhibiting and/or ROS-inhibiting therapeutic. | 2017-09-28 |
20170275707 | Markers for Breast Cancer - Correlations between polymorphisms and breast cancer are provided. Methods of diagnosing, prognosing, and treating breast cancer are provided. Systems and kits for diagnosis, prognosis and treatment of breast cancer are provided. Methods of identifying breast cancer modulators are also described. | 2017-09-28 |
20170275708 | METHODS FOR MAKING PLANTS RESISTANT TO FUNGAL PATHOGENS - This invention relates to polynucleotide sequences encoding genes that can confer resistance to the plant pathogen | 2017-09-28 |
20170275709 | COMPOSITIONS AND METHODS FOR THE DETECTION OF THE SHRUNKEN2-R MUTATION IN MAIZE - The present invention relates to compositions and methods for detecting the shrunken2-R (sh2-R) mutation and identifying maize plants, maize plant parts and/or maize germplasm having the sh2-R mutation. | 2017-09-28 |
20170275710 | NOVEL METHOD FOR SIMULTANEOUS DETECTION AND DISCRIMINATION OF BACTERIAL, FUNGAL, PARASITIC AND VIRAL INFECTIONS OF EYE AND CENTRAL NERVOUS SYSTEM - The present invention relates to the diagnostic methods for identification of the single causative agent or more than one causative agent of ocular and nervous system infections among many probable pathogens, which can cause the infection. All the pathogens affecting a discrete area of eye or nervous system generally cause same clinical manifestations or syndromes. The present invention relates to detection and discrimination of the pathogen among the set of probable pathogens in a single test without resorting to a battery of tests each being directed at detection of one pathogen. The current invention aims at the syndrome based diagnostic replacing the diagnostics based on detection of individual | 2017-09-28 |
20170275711 | BROAD DETECTION OF DENGUE VIRUS SEROTYPES - Processes for detecting Dengue virus (DENV) nucleic acid in a sample are provided including producing an amplification product by amplifying DENV nucleotide sequence and detection of an amplification by hybridization of a probe or other technique. The processes use primers or probes with introduced mutations and or degenerate bases that provide excellent superiority in detection and serotyping of DENV in a sample. | 2017-09-28 |
20170275712 | METHODS OF DETECTING INFLUENZA - Compositions and methods for detecting influenza are provided. | 2017-09-28 |
20170275713 | COMPOSITIONS AND METHODS FOR TREATING LEATHER - The present disclosure provides a composition for treating leather and similar materials. The composition comprises from about 40% (w/w) to about 60% (w/w) of an oil obtained from natural sources; from about 1% (w/w) to about 15% (w/w) of a wax obtained from natural sources; and from about 20% (w/w) to about 50% (w/w) of water. The present disclosure also provides a method for treating a leather article and a process for the preparation of an emulsion composition. | 2017-09-28 |
20170275714 | MIXTURE, USE OF THIS MIXTURE, AND METHOD FOR CONDITIONING A SLAG LOCATED ON A METAL MELT IN A METALLURGICAL VESSEL IN IRON AND STEEL METALLURGY - The invention relates to a mixture to be introduced into the slag located on a metal melt in iron and steel metallurgy, the use of such a mixture, and a method for conditioning a slag located on a metal melt in a metallurgical vessel, for example in a converter, in an electric arc furnace or in a ladle, in iron and steel metallurgy. | 2017-09-28 |
20170275715 | HIGH-STRENGTH SEAMLESS STEEL PIPE FOR OIL COUNTRY TUBULAR GOODS AND METHOD OF PRODUCING THE SAME (AS AMENDED) - Provided is a high-strength seamless steel pipe having the composition which contains, by mass %, 0.20 to 0.50% C, 0.05 to 0.40% Si, 0.3 to 0.9% Mn, 0.015% or less P, 0.005% or less S, 0.005 to 0.1% Al, 0.008% or less N, 0.6 to 1.7% Cr, 0.4 to 1.0% Mo, 0.01 to 0.30% V, 0.01 to 0.06% Nb, 0.0003 to 0.0030% B, and 0.0030% or less O (oxygen). The high-strength seamless steel pipe has the microstructure where a volume fraction of a tempered martensitic phase is 95% or more, and prior austenitic grains have a grain size number of 8.5 or more, and a segregation degree index Ps which is defined by a formula Ps=8.1 (X | 2017-09-28 |
20170275716 | NANOCRYSTALLINE BAINITIC STEELS, SHAFTS, GAS TURBINE ENGINES, AND METHODS OF MANUFACTURING NANOCRYSTALLINE BAINITIC STEELS - A nanocrystalline bainitic steel consisting of, by weight percentage: 0.3% to 0.6% carbon; 9.0% to 20.0% nickel; up to 10% cobalt; 1.0% to 4.5% aluminium; up to 0.5% molybdenum; up to 0.5% manganese; up to 0.5% tungsten; up to 3.0% chromium; and the balance being iron and impurities. | 2017-09-28 |
20170275717 | EXPLOSIVE HARDENING OF TRACK SHOES - A component of a crawler type machine is hardened by explosive depth hardening. The component is typically a crawler track shoe ( | 2017-09-28 |
20170275718 | HIGH-STRENGTH STEEL SHEET - Provided is a steel sheet with excellent abrasion resistance as well as excellent low-temperature toughness and ductility of a base material while having a high strength of a tensile strength of 1,100 MPa or more. The steel sheet is a high-strength steel sheet having a tensile strength of 1,100 MPa or more, wherein the components in the steel satisfy a defined composition, A-value represented by a defined formula (1) is 0.0015 or less, while E-value represented by a defined formula (3) is 0.95 or more, and a Brinell hardness HBW (10/3000) in a position at a depth of 2 mm from a surface of the steel sheet is 360 or more and 440 or less. | 2017-09-28 |
20170275719 | HIGH-TOUGHNESS HOT-ROLLING HIGH-STRENGTH STEEL WITH YIELD STRENGTH OF 800 MPA, AND PREPARATION METHOD THEREOF - A high-toughness hot-rolled high-strength steel with a yield strength of Grade 800 MPa with its chemical components, in weight percentages, being C 0.02-0.05%, Si≦0.5%, Mn 1.5-2.5%, P≦0.015%, S≦0.005%, Al 0.02-0.10%, N≦0.006%, Nb 0.01-0.05%, Ti 0.01-0.03%, 0.03%≦Nb+Ti≦0.06%, Cr 0.1%-0.5%, Mo 0.1-0.5%, B 0.0005-0.0025%, and the balance of Fe and unavoidable impurities, and a preparation method thereof. The present invention acquires, via direct quenching, an ultra-low carbon martensite structure with a yield strength of 800 Mpa and an impact energy of more than 100J under a temperature of −80° C. | 2017-09-28 |
20170275720 | METHOD OF MANUFACTURING HOT ROLLED STEEL SHEET FOR SQUARE COLUMN FOR BUILDING STRUCTURAL MEMBERS - A method of manufacturing a hot rolled steel sheet for a square column for building structural members includes a hot rolling step, a cooling step, and a coiling step performed on a steel to form a hot rolled steel sheet, wherein the steel has a composition containing, in terms of % by mass, C: 0.07 to 0.18%, Mn: 0.3 to 1.5%, P: 0.03% or less, S: 0.015% or less, Al: 0.01 to 0.06%, N: 0.0006% or less, and the balance being Fe and unavoidable impurities. | 2017-09-28 |
20170275721 | METHOD FOR PRODUCING HOT-FORMED STEEL SPRINGS - A process for producing a spring or torsion bar from a steel wire by hot forming may involve providing a steel wire; thermomechanically forming the steel wire; cooling the steel wire thermomechanically; cutting the steel wire to length to give rods; heating the rods; hot forming the rods; and tempering the rods to give a spring or torsion bar, comprising quenching the rods to give a spring or torsion bar to a first cooling temperature, reheating the spring or torsion bar to a first annealing temperature, and cooling the spring or rod to a second cooling temperature. Further, in some examples, the cooling of the steel wire may be cooled to a temperature below a minimum recrystallization temperature such that at least a partly ferritic-pearlitic structure is established in the steel wire.” | 2017-09-28 |
20170275722 | FERRITIC STAINLESS STEEL SHEET - Provided is a ferritic stainless steel sheet having excellent corrosion resistance and workability equal to or better than SUH409L. The ferritic stainless steel sheet contains 0.025% or less C, 0.01% to 1.00% Si, 0.05% to 1.00% Mn, 0.020% to 0.040% P, 0.030% or less S, 0.001% to 0.100% Al, 12.5% to 14.4% Cr, 0.01% to 0.80% Ni, 0.11% to 0.40% Ti, 0.010% to 0.100% Nb, and 0.020% or less N by mass %, the remainder being Fe and inevitable impurities. | 2017-09-28 |
20170275723 | FERRITE-BASED STAINLESS STEEL WITH HIGH RESISTANCE TO CORROSIVENESS CAUSED BY EXHAUST GAS AND CONDENSATION AND HIGH BRAZING PROPERTIES AND METHOD FOR MANUFACTURING SAME - This ferritic stainless steel contains, by mass %, C: 0.001% to 0.030%; Si: 0.01% to 1.00%, Mn: 0.01% to 2.00%, P: 0.050% or less, S: 0.0100% or less, Cr: 11.0% to 30.0%, Mo: 0.01% to 3.00%, Ti: 0.001% to 0.050%, Al: 0.001% to 0.030%, Nb: 0.010% to 1.000%, and N: 0.050% or less, with a remainder being Fe and inevitable impurities, wherein an amount of Al, an amount of Ti, and an amount of Si (mass %) satisfy Al/Ti≧8.4Si−0.78. | 2017-09-28 |
20170275724 | COLD ROLLED HIGH STRENGTH LOW ALLOY STEEL - A high strength low alloy steel. The high strength low alloy steel strip, sheet or blank, coated with zinc or a zinc alloy, has the following composition in weight %:
| 2017-09-28 |
20170275725 | FERRITIC STAINLESS STEEL FOIL AND METHOD FOR MANUFACTURING THE SAME (AS AMENDED) - Provided are a ferritic stainless steel foil excellent in terms of corrugating workability, shape change resistance at a high temperature, and manufacturability and a method for manufacturing the steel foil. A ferritic stainless steel foil having a chemical composition containing, by mass % , C: 0.020% or less, Si: 2.0% or less, Mn: 1.0% or less, S: 0.010% or less, P: 0.050% or less, Cr: 10.0% or more and 25.0% or less, Ni: 0.05% or more and 0.50% or less, Ti: 0.14% or more and 0.25% or less, Al: 0.001% or more and 0.10% or less, V: 0.02% or more and 0.10% or less, N: 0.020% or less, and the balance being Fe and inevitable impurities, and a Vickers hardness of higher than 200 and lower than 350. | 2017-09-28 |
20170275726 | HIGH-STRENGTH GALVANIZED STEEL SHEET EXCELLENT IN STRETCH-FLANGE FORMABILITY, IN-PLANE STABILITY OF STRETCH-FLANGE FORMABILITY, AND BENDABILITY AND METHOD FOR MANUFACTURING THE SAME - Provided are a high-strength galvanized steel sheet containing 0.12% to 0.25% C, 0.01% to 1.00% Si, 1.5% to 4.0% Mn, 0.100% or less P, 0.02% or less S, 0.01% to 0.10% Al, 0.001% to 0.010% N, 0.005% to 0.100% Ti, and 0.0005% to 0.0050% B, the remainder being Fe and inevitable impurities, Ti>4N being satisfied. The high-strength galvanized steel sheet contains 80% to 100% martensite in terms of area fraction, 5% or less (including 0%) polygonal ferrite in terms of area fraction, and less than 3% (including 0%) retained austenite in terms of area fraction. The average hardness of martensite is 400 to 500 in terms of Vickers hardness (Hv). The average grain size of martensite is 20 μm or less. The standard deviation of the grain size of martensite is 7.0 μm or less. | 2017-09-28 |
20170275727 | THICK STEEL PLATE HAVING GOOD MULTIPASS WELD JOINT CTOD CHARACTERISTICS AND METHOD FOR MANUFACTURING THE SAME - A steel plate comprising, by mass %: C: 0.03% to 0.12%, Si: 0.5% or less, Mn: 1.0% to 2.0%, P: 0.015% or less, S: 0.0005% to 0.0050%, Al: 0.005% to 0.060%, Ni: 0.5% to 2.0%, Ti: 0.005% to 0.030%, N: 0.0015% to 0.0065%, 0: 0.0010% to 0.0050%, Ca: 0.0005% to 0.0060%, and optionally one or two or more of Cu and the like. Ti/N, Ceq, Pcm, and ACR are in particular ranges, a base metal of the plate has an effective grain size of 20 μm or less at half the thickness of the plate, and the plate contains a particular number of complex inclusions at ¼ and ½ of the thickness of the plate. The complex inclusions comprise a sulfide containing Ca and Mn and an oxide containing Al and having an equivalent circular diameter of 0.1 μm or more. | 2017-09-28 |
20170275728 | STEEL FOR SOLID OXIDE FUEL CELLS AND MANUFACTURING METHOD THEREOF - There is provided a steel for solid oxide fuel cells which contains Zr and has a composition balance which allows a thin plate to stably obtain excellent oxidation resistance. The steel for solid oxide fuel cells contains more than 0 and not more than 0.05 mass % of C, 0.05 mass % or less of N, 0.01 mass % or less of O, 0.2 mass % or less of Al, 0.15 mass % or less of Si, 0.1 to 1.0 mass % of Mn, 20.0 to 25.0 mass % of Cr, more than 0 mass % and not more than 1.0 mass % of Ni, 0.02 to 0.12 mass % of La, 0.1 to 0.5 mass % of Zr, 0.15 to 0.5 mass % of La+Zr, and Fe and impurities as a remainder. The following relational formula is satisfied, and an Fe and Zr-containing intermetallic compound viewed in a ferrite matrix is 1.1 mass % or less in terms of a visual field area ratio. | 2017-09-28 |
20170275729 | METHOD OF AND FOR PRODUCING HEAVY PLATES - In the context of a method for producing heavy plate ( | 2017-09-28 |
20170275730 | HEARTH ROLL FOR CONTINUOUS ANNEALING FURNACES, AND METHOD FOR MANUFACTURING SAME - The present invention provides a hearth roll for heat treatment furnaces, which has excellent build-up resistance, has a hexavalent-chromium-free thermal spray coating film formed on the roll surface thereof and is safe for the environment. A method for manufacturing a hearth roll for continuous annealing furnaces includes a first step of applying an aqueous solution containing chromium phosphate onto a thermal spray coating film formed on the roll surface of a hearth roll or impregnating the thermal spray coating film with the aqueous solution; and a second step of burning the hearth roll. | 2017-09-28 |
20170275731 | HEARTH ROLL AND CONTINUOUS ANNEALING FACILITY - Provided is a hearth roll for supporting and conveying a steel sheet in a continuous annealing furnace, wherein all of a shaft portion and a roil main body are made from one or more ceramic materials, preferably constituted with concentric ceramic layers of different ceramic materials centering on the rotation shaft of the roll. The hearth roll has not only an excellent pickup resistance but also a long roll life free from maintenance for long periods. Also provided is a continuous annealing facility using the hearth roll in at least one of a heating zone, a soaking zone and a cooling zone. | 2017-09-28 |
20170275732 | METHOD FOR EXTRACTION AND SEPARATION OF RARE EARTH ELEMENTS - A method for extracting and separating rare earth elements comprising providing a rare earth-containing ore or tailings, grinding the rare earth-containing ore to form powdered ore; leaching the powered ore with at least one mineral acid, forming a leach solution comprising at least one metal ion, rare earth elements and a solid material, separating the solid material from the leach solution to form aqueous-metal concentrate, precipitating the aqueous-metal concentrate to selectively remove the metal ion from the leach solution and obtain a precipitate of the rare earth elements; heating the precipitate of the rare earth elements in air to form oxide of the rare earth elements, mixing the oxide of the rare earth elements with an ammonium salt and heating in a dry air/nitrogen, forming a mixture of anhydrous rare earth salts in an aqueous solution, and separating the rare earth elements from the aqueous solution by means of an electrowinning process. | 2017-09-28 |
20170275733 | METHOD FOR PLATINUM RECOVERY FROM MATERIALS CONTAINING RHENIUM AND PLATINUM METALS - The present disclosure relates to hydrometallurgical methods for the isolation and recovery of platinum from rhenium-containing materials, and more particularly, from superalloys containing rhenium, platinum, and other metals. The disclosure also relates to apparatuses capable of carrying out the hydrometallurgical methods and the product streams generated from the methods and apparatuses. | 2017-09-28 |
20170275734 | Hydrothermal process for the treatment of lead glass with recovery of lead metal, soluble and insoluble silicates and silica - There is described a process completely performed in aqueous phase, which provides a heat etching of lead glass with aqueous solutions of strong alkali followed by an electrolytic treatment of the suspension so obtained, in order to recover metallic lead and obtain soluble silicates, separated from insoluble silicates, both lead-free. The process also provides for the production of pure silica, derived from the soluble silicates, and a possible use thereof to increase the ratio between silica and sodium oxide, which characterizes the specifications of the soluble silicates. The electrolysis for the recovery of metallic lead is implemented in a cell in which the polarity of the electrodes is periodically reversed, to obtain the detachment of the metallic lead deposited on the cathodes. | 2017-09-28 |
20170275735 | ECONOMICALLY ALLOYED TITANIUM ALLOY WITH PREDICTABLE PROPERTIES - The invention relates to the field of non-ferrous metallurgy, and more particularly to the creation of titanium alloys which, by virtue of their properties, are economical to use not only in traditional, particularly military, fields, but also in civilian fields of industry. The alloy contains 0.1-3.0 Al, 0.3-3.0 Fe, 0.1-1.0 Cr, 0.05-1.0 Ni, 0.02-0.3 Si, 0.02-0.2 N, 0.05-0.5 0, 0.02-0.1 C, and the remainder Ti. The technical result is the production of a commercially viable titanium alloy with guaranteed stable, predictable properties which is manufactured using, low-grade titanium sponge. This result is achieved in that the alloying elements used include impurity elements contained in the low-grade sponge, as well as alloying additives, separately added to the charge. The titanium-based alloy has a lower price compared to existing commercial alloys and, furthermore, the composition of the alloy is selected in accordance with a prescribed level of physical and mechanical properties and fabrication characteristics. | 2017-09-28 |
20170275736 | Ni-BASE SUPER ALLOY - There is provided an Ni-base super alloy which is used for airplane engines and gas turbines for power generation and has favorable mechanical properties at high temperature. The Ni-base super alloy contains 0.001 to 0.1 mass % of C, 1.0 to 4.0 mass % of Al, 2.0 to 4.5 mass % of Ti, 12.0 to 18.0 mass % of Cr, 11.1 to 18.0 mass % of Co, 1.2 to 12.0 mass % of Fe, 1.5 to 6.5 mass % of Mo, 0.5 to 6.0 mass % of W, 0.1 to 3.0 mass % of Nb, 0.001 to 0.05 mass % of B, 0.001 to 0.1 mass % of Zr, and Ni and impurities as a remainder. | 2017-09-28 |