42nd week of 2015 patent applcation highlights part 26 |
Patent application number | Title | Published |
20150291942 | MODIFIED TRANSPOSASES FOR IMPROVED INSERTION SEQUENCE BIAS AND INCREASED DNA INPUT TOLERANCE - Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same. | 2015-10-15 |
20150291943 | Polypeptides Having Chlorophyllase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having chlorophyllase activity, and polynucleotides encoding the polypeptides. The invention further relates to the use of such polypeptides in detergent and/or in cleaning processes. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. | 2015-10-15 |
20150291944 | COMPOSITIONS AND METHODS COMPRISING A LIPOLYTIC ENZYME VARIANT - The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention. | 2015-10-15 |
20150291945 | CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF - The present invention provides nucleotide sequences of | 2015-10-15 |
20150291946 | Methods and Vectors for Generating Asialylated Immunoglobulins - The properties of an Fc-containing protein, for example, an antibody, are controlled by altering the sialylation of the oligosaccharides in the Fc region by transfecting the cell line expressing the Fc-containing protein with a vector sequence encoding a sialidase. The modified Fc-containing proteins have therapeutic utility in diseases or conditions in which it is desirable to control the affinity for one or more of the FcγRI, FcγRIIA, and FcγRIIIA receptors, ADCC activity, macrophage or monocyte activation, serum half-life, and avidity. | 2015-10-15 |
20150291947 | CELLOBIOHYDROLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2015-10-15 |
20150291948 | NUCLEIC ACIDS USEFUL FOR INTEGRATING INTO AND GENE EXPRESSION IN HYPERTHERMOPHILIC ACIDOPHILIC ARCHAEA - The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration. | 2015-10-15 |
20150291949 | DETERGENT COMPOSITIONS COMPRISING SUBTILASE VARIANTS - detergent compositions comprising subtilase variants and methods for obtaining such detergent compositions are provided herein. The detergent compositions further may be used, especially in laundry or in hard surface cleaning applications. | 2015-10-15 |
20150291950 | PHOTOCLEAVABLE LINKER - There are provided, inter alia, photolabile compounds and methods useful for the formation of dimers of biological molecules and subsequent dissociation of the dimers. | 2015-10-15 |
20150291951 | GENOME EDITING - This document provides materials and methods for editing a genome (e.g., an animal genome in vivo). For example, methods and materials for using a targeted endonuclease and a donor nucleic acid having a length within a particular range (e.g., from 20 to 95 nucleotides) to edit a genome are provided. | 2015-10-15 |
20150291952 | RNA APTAMER ISOLATION VIA DUAL-CYCLE (RAPID) SELECTION - The present invention relates to a method for selecting an aptamer for a target molecule. The method involves providing a random oligonucleotide library comprising a plurality of unique random sequence oligonucleotides; providing a target mixture comprising at least one target molecule; and subjecting the random oligonucleotide library and the target mixture to at least one round of an aptamer isolation protocol to yield at least one aptamer for the target molecule, wherein a round of the aptamer isolation protocol comprises at least one selection cycle followed by an amplification cycle. The present invention also relates to systems and devices for implementing or performing the method of the present invention. The present invention further relates to using the method to isolate aptamers for high-throughput sequencing analysis and other aptamer analysis protocols. | 2015-10-15 |
20150291953 | METHODS AND KITS FOR NUCLEIC ACID SAMPLE PREPARATION FOR SEQUENCING - The present disclosure relates to methods and kits for DNA library construction, particularly for consistent and reproducible DNA sequencing. | 2015-10-15 |
20150291954 | ORGANIC COMPOSITIONS TO TREAT BETA-CATENIN-RELATED DISEASES - The present disclosure relates to RNAi agents useful in methods of treating Beta-Catenin-related diseases such as adenomatous polyposis of the colon, colorectal cancer, basal cell carcinoma, breast cancer, kidney cancer, Wilms tumors, medulloblastoma, ovarian cancer, adrenocortical tumors, gastric cancer, liver cancer, melanoma, pancreatic cancers, prostate cancer, renal cancer, ectopic teeth and taste papillae, skin cancer, pilomatrixoma, anaplastic thyroid carcinoma, and uterine carcinosarcoma, oligodontia, osteoporosis, ageing, degenerative diseases, bedsores, chronic wounds and impaired wound healing, and similar and related diseases, using a therapeutically effective amount of a RNAi agent to Beta-Catenin. | 2015-10-15 |
20150291955 | ANTISENSE COMPOUNDS TARGETING GENES ASSOCIATED WITH FIBRONECTIN - Provided are compounds comprising oligonucleotides complementary to a fibronectin transcript. Certain such compounds are useful for hybridizing to a fibronectin transcript, including but not limited to a fibronectin transcript in a cell. Such hybridization results in modulation of splicing of the fibronectin transcript. Such compounds are used to treat one or more symptoms associated with fibrosis. Such compounds are used to treat one or more symptoms associated with renal fibrosis. | 2015-10-15 |
20150291956 | RNA TARGETED TO BETA CATENIN - A double stranded RNA (dsRNA) molecule targeted to beta catenin includes a duplex region having a sense region and an antisense region at least substantially complementary to the sense region. The sense region and the antisense region each have between 18 and 30 nucleotides. The antisense region includes a nucleotide sequence that is fully complementary to at least 15 contiguous nucleotides of any one of SEQ ID NOs: 1-24. | 2015-10-15 |
20150291957 | METHODS AND COMPOSITIONS TO PRODUCE ss-RNAi ACTIVITY WITH ENHANCED POTENCY - Compositions and methods for down modulating expression of target nucleic acids are disclosed. This invention relates to the fields of medicine, drug development and modulation of target nucleic acid expression. More specifically, the invention provides compositions and methods of use thereof that facilitate the modulation of target nucleic acid expression using novel oligonucleotide based drugs that act through an inhibitory RNA (RNAi) mechanism of action. | 2015-10-15 |
20150291958 | ANTI APOB ANTISENSE CONJUGATE COMPOUNDS - The present invention relates to conjugates of LNA antisense oligonucleotides (oligomers) that target ApoB. | 2015-10-15 |
20150291959 | Diagnosis and Treatment of Endometriosis - The invention discloses a diagnosis of endometriosis, by determining a level of miR-199a-5p as a biomarker to diagnose endometriosis. The invention also discloses a treatment of endometriosis. | 2015-10-15 |
20150291960 | MODULATION OF CD40 EXPRESSION - Disclosed herein are antisense compounds and methods for decreasing CD40. Examples of disease conditions that can be ameliorated with the administration of antisense compounds targeted to CD40 include hyperproliferative disorders, graft versus host disease (GVHD), graft rejection, asthma, airway hyperresponsiveness, chronic obstructive pulmonary disease (COPD), multiple sclerosis (MS), systemic lupus erythematosus (SLE), and certain forms of arthritis. | 2015-10-15 |
20150291961 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX - Isolation or in vitro assembly of the Cas9-crRNA complex of the | 2015-10-15 |
20150291962 | EXTRACTION, PREPARATION, AND APPLICATION OF PLANT MICRO-RIBONUCLEIC ACID - An isolated plant-functional microRNA or an extract containing said plant-functional microRNA, a preparation method therefor, and an application thereof are provided. The plant-functional microRNA originates from the endogenous microRNA of a plant, is present in a water-soluble and/or fat-soluble extract of said plant, and is capable of regulating non-plant target-genes. | 2015-10-15 |
20150291963 | SORTILIN 1 IS A NOVEL INDUCER OF VASCULAR CALCIFICATION - The invention relates to methods for decreasing, inhibiting, preventing, or reducing calcification by inhibiting sortilin 1. | 2015-10-15 |
20150291964 | Methods and Compositions for treatment of cancer by inhibition of NR2F6 - The current invention discloses compositions of matter, protocols and methods of use of treatment for cancer and other diseases of aberrant cellular proliferation and differentiation by inhibiting expression of NR2F6 or activity thereof. In one embodiment, administration of synthetic oligonucleotides that induce RNA interference mediated degradation of the nuclear receptor NR2F6 in human or animal patients is performed at a sufficient concentration or frequency to achieve regression of tumor. | 2015-10-15 |
20150291965 | ENGINEERING AND OPTIMIZATION OF SYSTEMS, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS - The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity. | 2015-10-15 |
20150291966 | INDUCIBLE DNA BINDING PROTEINS AND GENOME PERTURBATION TOOLS AND APPLICATIONS THEREOF - The present invention generally relates to methods and compositions used for the spatial and temporal control of gene expression that may use inducible transcriptional effectors. The invention particularly relates to inducible methods of altering or perturbing expression of a genomic locus of interest in a cell wherein the genomic locus may be contacted with a non-naturally occurring or engineered composition comprising a deoxyribonucleic acid (DNA) binding polypeptide. | 2015-10-15 |
20150291967 | COUPLING HERBICIDE RESISTANCE WITH TARGETED INSERTION OF TRANSGENES IN PLANTS - The present invention relates to methods allowing the targeted insertion of transgenes into a plant genome at desired loci by using homologous recombination combined with rare-cutting endonucleases without the need of inserting an exogenous selectable marker. | 2015-10-15 |
20150291968 | SB-ACTIN TERMINATOR SEQUENCE FOR GENE EXPRESSION IN PLANTS - The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences from | 2015-10-15 |
20150291969 | COMPOSITIONS FOR REDUCED LIGNIN CONTENT IN SORGHUM AND IMPROVING CELL WALL DIGESTIBILITY, AND METHODS OF MAKING THE SAME - RNAi vectors comprising a fragment of the SbCSE polynucleotide sequence and transgenic plants, e.g. transgenic | 2015-10-15 |
20150291970 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES, AND METHODS OF USING SAME FOR INCREASING NITROGEN USE EFFICIENCY, YIELD, GROWTH RATE, VIGOR, BIOMASS, OIL CONTENT, AND/OR ABIOTIC STRESS TOLERANCE - Provided are methods of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3047; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO:468-784, 3048-4333, 4335-4682. Also provided isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, 785-3047, which can be used to increase nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant. | 2015-10-15 |
20150291971 | METHOD FOR PRODUCING A TRANSGENIC CELL WITH INCREASED GAMMA-AMINOBUTYRIC ACID (GABA) CONTENT - This invention relates generally to a method for producing a transgenic cell with increased gamma-aminobutyric acid (GABA) content as compared to a corresponding non-transformed wild type cell. | 2015-10-15 |
20150291972 | Modification of Lignin Biosynthesis - The present invention relates to the modification of lignin biosynthesis in plants and, more particularly, to enzymes involved in the lignin biosynthetic pathway and nucleic acids encoding such enzymes. | 2015-10-15 |
20150291973 | METHOD FOR PLANT IMPROVEMENT - The invention relates to the field of plant improvement, in particular of the improvement of yield for plants, by using a transgene containing the BETL9 promoter driving expression of the MRP1 protein. | 2015-10-15 |
20150291974 | BROAD-SPECTRUM RESISTANCE TO BACTERIAL BLIGHT - The present invention relates to synthetic promoter and a synthetic gene which confers broad-spectrum disease resistance to Xanthomonands in plants. The present invention also relates to transgenic plants containing the synthetic gene and plants derived by crossing plants with such transgenic plants. More specifically, the synthetic promoter is a synthetic Xa10 promoter and the synthetic gene is a synthetic Xa10 gene which contains the synthetic Xa10 promoter. The resistance is resistance to bacterial blight and the plants are rice plants. | 2015-10-15 |
20150291975 | ENHANCED NUCLEIC ACID CONSTRUCTS FOR EUKARYOTIC GENE EXPRESSION - The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery. | 2015-10-15 |
20150291976 | ENHANCED NUCLEIC ACID CONSTRUCTS FOR EUKARYOTIC GENE EXPRESSION - The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery. | 2015-10-15 |
20150291977 | ENHANCED NUCLEIC ACID CONSTRUCTS FOR EUKARYOTIC GENE EXPRESSION - The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery. | 2015-10-15 |
20150291978 | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE - Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal. | 2015-10-15 |
20150291979 | NOVEL PSEUDOTYPED LENTIVIRAL PARTICLES AND THEIR USE IN THE IN VITRO TARGETED TRANSDUCTION OF UNDIFFERENTIATED PLURIPOTENT HUMAN EMBRYONIC STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS - The inventors developed novel pseudotyped lentiviral vector particles comprising a morbillivirus fusion (F) protein and a mutated hemagglutinin (H) protein of the measles virus (MeV) or the Edmonton strain of the measles virus (MeV | 2015-10-15 |
20150291980 | CELL CAPABLE OF PRODUCING ADENO-ASSOCIATED VIRUS VECTOR - According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies. | 2015-10-15 |
20150291981 | Production of Alkenes From 3-Hydroxy-1-Carboxylic Acids Via 3-Sulfonyloxy-1-Carboxylic Acids - The application describes a method for producing alkenes (for example propylene, ethylene, 1-butylene, isobutylene, isoamylene, butadiene or isoprene) from 3-hydroxy-1-carboxylic acids via 3-sulfonyloxy-1-carboxylic acids. | 2015-10-15 |
20150291982 | SYSTEM AND METHOD FOR CONTINUOUS ENZYME ADDITION TO A FERMENTATION PROCESS - A method for controlling a fermentation process includes injecting a mash into a fermenter and injecting an enzymatic additive into the fermenter on a continuous basis. The enzymatic additive is injected on a continuous basis during at least two time periods: during a batch fill and after the batch fill. The method may be used to control the fermentation processes of one or more fermenters operating in parallel. | 2015-10-15 |
20150291983 | CELL SUITABLE FOR FERMENTATION OF A MIXED SUGAR COMPOSITION - The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome. | 2015-10-15 |
20150291984 | PROCESSES FOR PRODUCING FERMENTATION PRODUCTS - The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention. | 2015-10-15 |
20150291985 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE - A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. | 2015-10-15 |
20150291986 | ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION - The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate. | 2015-10-15 |
20150291987 | PATHWAYS TO ADIPATE SEMIALDEHYDE AND OTHER ORGANIC PRODUCTS - Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided. | 2015-10-15 |
20150291988 | METHOD FOR OBTAINING SUGAR DERIVATIVES - A process for converting a sugar from a hemicellulose-containing material into the form of a compound having at least one ionic binding site, which is characterized in that the hemicellulose-containing material is hydrolyzed enzymatically or non-enzymatically and the obtained hydrolysate is subjected to a conversion involving at least one enzymatic step, wherein sugars are released and the released sugars are converted into compounds having at least one ionic binding site, and the use of such a process. | 2015-10-15 |
20150291989 | REACTOR FOR CONTINUOUS SACCHARIFICATION OF HIGH-SOLID BIOMASS - A reactor for continuous saccharification of biomass having a high solid content comprises perforated plates and stirring means, in which the perforated plates includes nozzles for feeding additives, including a saccharification enzyme, and a sensor, which communicate with the holes of the perforated plates. | 2015-10-15 |
20150291990 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2015-10-15 |
20150291991 | METHODS AND COMPOSITIONS RELATING TO AMYLOIDOGENIC POLYPEPTIDES - The technology described herein relates to the expression of polypeptides, e.g. heterologous polypeptides using a bipartite curli signal sequence. | 2015-10-15 |
20150291992 | METHOD AND APPARATUS FOR DETERMINING MICROORGANISMS IN A WATER SAMPLE - The invention relates to an apparatus and method for determining presence of microorganisms in, e.g., sea water or other water samples. Means are provided for taking a sample of the water to be tested, moving it through a series of reservoirs, and combining it with an indicator, such as a dye, which binds to DNA of any microorganisms in the sample. Any binding of indicator is measured by, e.g., a spectrofluorometer, or other device suitable for measuring the indicator. | 2015-10-15 |
20150291993 | System and Method for Detecting Biofilm Growth in Water Systems - A system and method for monitoring or detecting a level of biofilm growth in a fluid system and controlling operating parameters of the fluid system based a measured level of growth. The monitoring system and method comprises a dye injection system for periodically injecting dye into a portion of fluid from the fluid system, passing the portion of fluid though a narrow lumen tube to achieve laminar flow and using a light source and optical sensor to detect a transmission or emission indicating a level of biofilm growth in the tube corresponding to a level of growth on components in the fluid system. Information based upon the measurements or calculations made by the monitoring system may be used to manually or automatically alter various operating parameters to control the fluid system and aid in maintaining stable operation of the fluid system within preferred specifications. | 2015-10-15 |
20150291994 | MICROORGANISM ENUMERATION METHODS - This disclosure provides methods for enumeration of microorganisms within a freeze-dried powder suspected of containing Lactobacilli or Bifidobacteria. | 2015-10-15 |
20150291995 | HIGH-THROUGHPUT MUTAGENIZED CELL SCREENING SYSTEM FOR SELECTIVE SINGLE CELL EXTRACTION - The subject invention pertains to a microfluidic apparatus and methods for screening and isolating a target cell from a population of cells. The apparatus comprises a first microfluidic layer comprising microfluidic channels; a second microfluidic layer comprising microfluidic channels; and a microfluidic cell analysis layer comprising a top hanging blocking structure located directly below each location where the first layer microfluidic channels overlap with the second layer microfluidic channels and a cell trap juxtaposed to each of the top hanging blocking structures. The top hanging blocking structures can close or open the juxtaposed cell trap when either or both the first or second layer microfluidic channels located directly above the top hanging blocking structure are sufficiently pressurized and/or sufficiently depressurized. The methods for screening and isolating a target cell from a population of cells comprise screening the population of cells using the apparatus and isolating the target cell interest therefrom. | 2015-10-15 |
20150291996 | METHODS AND REAGENTS FOR GLYCOPROTEOMICS - Methods, reagents, and kits for the reversible immobilization of glycoproteins to a solid support, the release and capture of a glycan portion of the glycoprotein, and the subsequent release and capture of the polypeptide portion of the glycoprotein are provided. The disclosure also provides suitable solid support materials, surface chemistries, and devices for use in the disclosed methods. The methods, reagents, kits, and devices provided herein are useful for the analysis of protein glycosylation, for example, in a diagnostic context, in the context of proteoglycomics, and in the context of producing glycosylated proteins for therapeutic purposes. | 2015-10-15 |
20150291997 | METHODS AND DEVICES FOR DETECTION OF RESISTANCE TO AN ENZYME INHIBITOR - Embodiments described herein relate to assay methods and kits for detecting resistance of any enzyme to its inhibitor due to functional alteration of the enzyme, comprising, conducting two or more reactions with two or more reagent mixes optionally containing substrates for the enzyme. The mixes are substantially similar, except that one contains no enzyme inhibitor whereas the others contain an enzyme inhibitor being tested for resistance. The ratio of the signal from the reaction with an inhibitor to that from a reaction without an inhibitor is used to indicate whether the enzyme is resistant to the enzyme inhibitor and also determine the susceptibility or resistance of the enzyme to various inhibitors and further identify enzyme variants. Embodiments further relate to assay methods comprising only two reactions—one conducted in a mix containing the inhibitor and the other without the inhibitor. Further included are devices for conducting such assays. | 2015-10-15 |
20150291998 | METHOD FOR EVALUATION OF DRUG EFFICACY OF A MEDICINE HAVING A THERAPEUTIC OR PREVENTIVE EFFECT AGAINST A DISEASE RELATED TO EL ACTIVITY AND A METHOD FOR SCREENING AN INHIBITOR OF EL ACTIVITY - The present invention is related to a method for evaluation of drug efficacy of a medicine having a therapeutic or preventive effect against a disease related to EL activity wherein phosphatidylinositol or lysophosphatidylinositol is used as an indicator. The present invention is also related to a method for screening an inhibitor of EL activity using phosphatidylinositol and a kit for use in the method. | 2015-10-15 |
20150291999 | NUCLEIC ACID AMPLIFICATION AND USE THEREOF - The invention features compositions and methods that are useful for the measurement of the quantity of a nucleic acid target in a sample. | 2015-10-15 |
20150292000 | CONTROL FOR DIAGNOSTIC ASSAY - A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay. | 2015-10-15 |
20150292001 | Methods for Standardized Sequencing of Nucleic Acids and Uses Thereof - Methods for standardized sequencing of nucleic acids and uses thereof are described. The identification of genetic information is becoming a key piece of information for the diagnosis and treatment of many diseases. In order to make such diagnostic tool readily available, it is desired that this identification be as efficient and as inexpensive as possible. | 2015-10-15 |
20150292002 | Multiplex Amplification of Polynucleotides - The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis. | 2015-10-15 |
20150292003 | METHODS AND KITS FOR DETECTION OF A PATHOGEN IN SUGARCANE - Embodiments of the present invention provide a diagnostic approach utilizing quantitative polymerase chain reaction (PCR) to detect quantitatively a pathogen of the genus | 2015-10-15 |
20150292004 | DETECTION OF TOXIGENIC STRAINS OF CLOSTRIDIUM DIFFICILE - Primers and probes for detection of toxin-producing (toxigenic) strains of | 2015-10-15 |
20150292005 | NUCLEIC ACID MOLECULES FOR HIGHLY SENSITIVE DETECTION OF LIGANDS, SCREENING METHOD FOR NUCLEIC ACID MOLECULES, AND OPTIMIZATION METHOD FOR SENSITIVITY OF NUCLEIC ACID MOLECULES - It is an object of the present invention to provide nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is another object of the present invention to provide a screening method for nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin), and a method for screening for nucleic acid molecules used for the optimization of nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is a further object of the present invention to provide a method for effectively removing ligands from samples containing ligands (e.g., patulin). According to the present invention, there is provided a loop-structured nucleic acid molecule for detection of ligands (e.g., patulin) having a DNA aptamer and a DNAzyme, wherein the sequence is modified between the DNA aptamer region and the DNAzyme. | 2015-10-15 |
20150292006 | MULTIPLEX DECODING OF ARRAY SENSORS WITH MICROSPHERES - The invention relates to compositions and methods for multiplex decoding of microsphere array sensors. | 2015-10-15 |
20150292007 | Spatial Sequencing of Nucleic Acids Using DNA Origami Probes - A method of sequencing nucleic acids by probe hybridization and/or ligation is provided using DNA origami as a barcode for a nucleic acid probe. | 2015-10-15 |
20150292008 | SHORT CYCLE METHODS FOR SEQUENCING POLYNUCLEOTIDES - The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion. | 2015-10-15 |
20150292009 | Measurement and Comparison of Immune Diversity by High-Throughput Sequencing - A precise measurement of the immunological receptor diversity present in a sample is obtained by sequence analysis. Samples of interest are generally complex, comprising more than 10 | 2015-10-15 |
20150292010 | Methods and Compositions for Improved Fertilization and Embryonic Survival - Single nucleotide polymorphic sites at positions 19069 and 25402 of the bovine STAT3 gene are associated with improved fertilization rate and/or improved embryo survival rate. The interactions between these two polymorphisms, and between them and the bovine STAT1 gene and fertilization and early embryonic survival rates were also disclosed. The interactions between STAT3 SNPs, and between STAT1 and STAT3 SNP19069 were highly significant for embryonic survival rate. Also disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods. | 2015-10-15 |
20150292011 | Biomarkers for Diabetes and Usages Thereof - Biomarkers for diabetes and usages thereof are provided. And the biomarkers are nucleotides having polynucleotide sequences defined in SEQ ID NOs: 1-50. | 2015-10-15 |
20150292012 | BIOMARKERS FOR NOD2 AND/OR RIP2 ACTIVITY RELATED APPLICATION - A method of predicting RIP2 inhibitor efficacy in treating a subject with an inflammatory disorder includes obtaining a bodily sample from the subject, determining in the bodily sample the expression level(s) of at least one gene selected from the group consisting of cd40, Clec4E, clec5a, CxCL10, gpr84, Icam1, Irgl, Marcks11, pde4b, Ptges, Rasgrp1, and slc2a6,comparing the expression levels of the at least one gene with the corresponding control value(s), and characterizing the subject as being responsive to RIP2 inhibitor treatment if the expression levels of the at least one gene is increased compared to the corresponding control value(s). | 2015-10-15 |
20150292013 | NOVEL MIRNAS AS DIAGNOSTIC MARKERS - The invention relates to novel miRNA markers useful for diagnosis or therapy of disease, in particular for neuronal disorders such as Alzheimer's Disease (AD). | 2015-10-15 |
20150292014 | METHOD AND SYSTEM TO PREDICT RESPONSE TO TREATMENTS FOR MENTAL DISORDERS - The present inventions relates to methods and assays to predict the response of an individual to psychiatric treatment and to a method to improve medical treatment of a disorder, which responsive to treatment with a psychiatric treatment. | 2015-10-15 |
20150292015 | SELECTIVE REDUCTION OF ALLELIC VARIANTS - Disclosed herein are antisense compounds and methods for selectively reducing expression of an allelic variant of a huntingtin gene containing a single nucleotide polymorphism (SNP). Such methods, compounds, and composition are useful to treat, prevent, or ameliorate Huntington's Disease (HD). | 2015-10-15 |
20150292016 | NOVEL MARKERS FOR MENTAL DISORDERS - Methods and kits for diagnosing a psychiatric disorder such as schizophrenia, schizo-affective disorder, bipolar disorder or Tourette syndrome, or a predisposition thereto, in a subject, are disclosed. The methods and kits are based on the detection of the presence of SNP rs1156026, and/or of deletions located in introns of the DNAJC15 gene, in a biological sample from the subject. | 2015-10-15 |
20150292017 | Systems For Identifying Cosmetic Agents For Skin Care Compositions - A system for identifying connections between a cosmetic agent and at least one gene associated with a skin aging condition. The system includes a computer readable medium having stored thereon a plurality of instances and a skin aging gene expression signature. Each instance comprises an ordered list of identifiers representing a plurality of differentially expressed genes generated as a result of contact between the cosmetic agent and a human fibroblast cell or a human keratinocyte cell. The computing device includes computer-readable instructions for accessing the plurality of instances and the skin aging gene expression signature stored on the computer readable medium, comparing each of the instances to the skin aging gene expression signature, and assigning a connectivity score to each of the instances based on the comparison. | 2015-10-15 |
20150292018 | METHOD OF MAKING SKIN CARE COMPOSITIONS - A method for making a skin care composition by identifying connections between cosmetic agents and genes associated with one or more skin aging conditions. The method includes accessing a plurality of instances and at least one skin aging gene expression signature, comparing the at least one skin aging gene expression signature to the plurality of the instances, assigning a connectivity score to each instance based on the comparison and incorporating the cosmetic agent into a skin care composition when the connectivity score of the instance associated with the cosmetic agent has a negative correlation. | 2015-10-15 |
20150292019 | Array-based Translocation and Rearrangement Assays - Methods for detecting genomic rearrangements are provided. In one embodiment, methods are provided for the use of paired end tags from restriction fragments to detect genomic rearrangements. Sequences from the ends of the fragments are brought together to form ditags and the ditags are detected. Combinations of ditags are detected by an on-chip sequencing strategy that is described herein, using inosine for de novo sequencing of short segments of DNA. In another aspect, translocations are identified by using target specific capture and analysis of the captured products on a tiling array. | 2015-10-15 |
20150292020 | miRNA Fingerprint in the Diagnosis of COPD - MicroRNAs (miRNA) are a recently discovered class of small non-coding RNAs (17-14 nucleotides). Due to their function as regulators of gene expression they play a critical role both in physiological and in pathological processes, such as cancer. The present invention provides novel methods for diagnosing a state of health based on the determination of specific miRNAs that have altered expression levels in different conditions, e.g. disease states compared to healthy controls. | 2015-10-15 |
20150292021 | PROGNOSTIC METHODOLOGY - The invention concerns a prognostic method for determining at least one, or a combination, of the following: time to first treatment, response to treatment or overall survival for a patient presenting with a disease including or characterised by telomere shortening, comprising an assessment of the longest mean telomere length at which telomere end-end fusion events can be detected and then a determination of the mean telomere length in the fusogenic range (i.e. the range below said mean telomere length at which telomere end-end fusion events can be detected) and the subsequent use of the mean telomere length in the fusogenic range as a prognostic indicator. | 2015-10-15 |
20150292022 | NATURAL-KILLER/T-CELL LYMPHOMA (NKTCL) SUSCEPTIBILITY PREDICTION, DIAGNOSIS AND THERAPY - Natural-Killer T-Cell Lymphoma (NKTCL) susceptibility prediction, diagnosis and therapy. The invention relates to a method for predicting Natural Killer T-cell Lymphoma (NKTCL) susceptibility and/or diagnosing NKTCL in a subject comprising testing for JAK mutations. The invention also relates to a method of screening for candidate agents capable of treating NKTCL using a cell line comprising at least one JAK mutation. The invention includes an NKTCL animal model comprising at least one JAK mutation. The invention also includes JAK inhibitors for treating NKTCL. | 2015-10-15 |
20150292023 | Materials and Methods Related to NSAID Chemoprevention in Colorectal Cancer - Described herein are methods of preventing cancer, treating cancer, inhibiting tumor growth, predicting patient outcomes, optimizing dosage, and monitoring the efficacy of treatment. In particular, certain microRNAs and messenger RNAs are useful indicators of response to NSAID chemoprevention and therapy of patients having Lynch syndrome or hereditary non-polyposis colorectal cancer. | 2015-10-15 |
20150292024 | BIOMARKERS FOR BREAST CANCER PREDICTION AND DIAGNOSIS - This invention provides the applications of CST1 gene and its splice variants (sequence shown in SEQ ID No.48) and cystatin SN protein coded by CST1 gene (sequence shown in SEQ ID No.52) for the predictions and diagnoses of human breast cancers. The invention can be used for the diagnosis of breast cancer and breast cancer metastasis, for the analysis for the susceptibility for breast cancer, for the evaluation of the treatments, medicine, efficacy and prognosis for original or metastatic breast cancer patients, and for the risk assessment for breast cancer or its metastasis. The methods described in this invention are of significant sensitivity, specificity and reliability. The invention provides the capturers of CST1 gene, its splice variants and cystatin SN and their applications in manufacturing diagnostic reagents, kits and chips for breast cancers. The diagnostic reagents, kits and chips for breast cancer are included in this invention. | 2015-10-15 |
20150292025 | METHYLATION BIOMARKERS FOR PROSTATE CANCER - Different combinations of methylation status based biomarkers can be used to test for prostate cancer with high sensitivity and high specificity. | 2015-10-15 |
20150292026 | METHYLATION MARKERS PREDICTIVE FOR DRUG RESPONSE - Disclosed are methods for detecting expression and/or aberrant methylation patterns in genes such as the gene DCR1 and their potential to diagnose or prognose a cancer or to predict drug resistance/susceptibility. More specifically, the disclosure relates to oligonucleotides, primers, probes, primer pairs and kits to detect genes such as the gene DCR1, and in particular, methylated forms of genes such as the gene DCR1. The disclosure also relates to pharmacogenetic methods to diagnose or prognose a cancer, to determine suitable treatment regimens for cancer, and to determine methods for treating cancer patients based on expression and/or aberrant methylation patterns in genes such as the gene DCR1. | 2015-10-15 |
20150292027 | PAPANICOLAOU TEST FOR OVARIAN AND ENDOMETRIAL CANCERS - The recently developed liquid-based Papanicolaou (Pap) smear allows not only cytologic evaluation but also collection of DNA for detection of HPV, the causative agent of cervical cancer. We tested these samples to detect somatic mutations present in rare tumor cells that might accumulate in the cervix once shed from endometrial and ovarian cancers. A panel of commonly mutated genes in endometrial and ovarian cancers was assembled and used to identify mutations in all 46 endometrial or cervical cancer tissue samples. We were able also able to identify the same mutations in the DNA from liquid Pap smears in 100% of endometrial cancers (24 of 24) and in 41% of ovarian cancers (9 of 22). We developed a sequence-based method to query mutations in 12 genes in a single liquid Pap smear without prior knowledge of the tumor's genotype. | 2015-10-15 |
20150292028 | Genetic Assay to Determine Prognosis in Polycythemia Vera Patients - The presently disclosed subject matter provides a genetic assay to determine the prognosis in Polycythemia Vera (PV) patients with an indolent form of PV. This assay involves measuring certain messenger RNAs (mRNAs) in blood cells, such as white blood cells. In some embodiments, the cells are CD34+ cells. These mRNA levels are inserted into an algorithm that yields a predictive score of the risk of PV in the patient transforming from an indolent form to an aggressive form. | 2015-10-15 |
20150292029 | METHODS AND COMPOSITIONS FOR DIAGNOSING AND TREATING GASTRIC CANCER - The present invention relates to the field of gastric cancer. More specifically, the present invention provides methods and compositions for diagnosing and treating gastric cancer. In a specific embodiment, a method for diagnosing gastric cancer or a likelihood thereof in a patient comprises the steps of (a) providing a sample from the patient; (b) measuring the methylation levels of one or more biomarkers in the sample collected from the patient; and (c) predicting gastric cancer in the patient if the biomarkers are hypermethylated. In a more specific embodiment, the one or more biomarkers comprises tight junction protein claudin-1 1 (CLD-N11), the transcription factor BarH-like homeobox (BARX1), basonuclin1 (BNC1), Coagulation factor C homolog (COCH), filamin C gamma (FLNC), cytoglobin B (CYGB), glutamine-fructose-6-phospahe-transaminase-2 (GFPT2), heat-shock-70 kDa protein-6 (HSPA6), snail homolog 1(SNAL1), skin calmodulin-related factor (SCARF), and tumor protein p53 binding protein 2 (TP53BP2). | 2015-10-15 |
20150292030 | METHODS OF CHARACTERIZING AND TREATING MOLECULAR SUBSET OF MUSCLE-INVASIVE BLADDER CANCER - The present application discloses a method of diagnostic testing of primary tumors, circulating tumor cells, serum, and urine to detect high-risk bladder cancers. These results have immediate implications for prognostication and the clinical management of muscle-invasive bladder cancer. | 2015-10-15 |
20150292031 | TREATMENT OF ANGIOGENESIS DISORDERS - This invention concerns pathological angiogenesis and cancer, related treatment methods, and related compositions. Also disclosed are related diagnosis kits and methods. | 2015-10-15 |
20150292032 | TREATMENT OF CANCER BY TARGETING QUIESCENT CANCER CELLS - A method for treating a subject suffering from a neoplasm, comprising determining the expression level of a DYRK1 selected from DYRK1A or DYRK1B within a cell population in a sample of neoplastic cells and determining the quiescent state of these cells, and if the DYRK1 is expressed in the sample then administering an effective amount of a modulator of DYRK1 activity, either alone or as a part of a combination therapy. | 2015-10-15 |
20150292033 | METHOD OF DETERMINING CANCER PROGNOSIS - Provided is a method of predicting the prognosis of a patient with ovarian cancer by determining the total number of somatic exome mutations per genome (Nmut) and status of the BRCA1 and/or BRCA2 in the subject. | 2015-10-15 |
20150292034 | RECEPTOR GENE FOR PEPTIDE CANCER ANTIGEN-SPECIFIC T CELL - The invention provides the nucleotide sequence and amino acid sequence of the CDR3 domain of the T cell receptor (TCR) gene of a WT1-specific cytotoxic T cell (CTL) against WT1 protein. Also provided are a method for testing for and treating cancer using the nucleotide sequence and amino acid sequence, and a chip, primer set, kit, and device for testing for cancer comprising the nucleotide sequence and amino acid sequence. | 2015-10-15 |
20150292035 | RECEPTOR GENE FOR PEPTIDE CANCER ANTIGEN-SPECIFIC T CELL - The invention provides the nucleotide sequence and amino acid sequence of the CDR3 domain of the T cell receptor (TCR) gene of a WT1-specific cytotoxic T cell (CTL) against WT1 protein. Also provided are a method for testing for and treating cancer using the nucleotide sequence and amino acid sequence, and a chip, primer set, kit, and device for testing for cancer comprising the nucleotide sequence and amino acid sequence. | 2015-10-15 |
20150292036 | CANCER SCREENING BY DETECTION OF ULTRASTRUCTURAL AND MOLECULAR MARKERS - The present invention relates to detection of cancer, or assessment of risk of development thereof. In particular, the present invention provides compositions and methods detection of field carcinogenesis by identification of ultrastructural and molecular markers in a subject. | 2015-10-15 |
20150292037 | METHOD FOR DETERMINING LYMPH NODE METASTASIS IN CANCER OR RISK THEREOF AND RAPID DETERMINATION KIT FOR THE SAME - The objective of the present invention is to provide a method and a means of rapidly and reliably detecting lymph node metastasis in cancer or the risk of lymph node metastasis. Specifically, the present invention provides a method and a rapid determination kit for detecting lymph node metastasis in cancer or its risk by identifying a certain genetic polymorphism of the human CRP gene, and it is clinically significant in determining the treatment strategy, because effective prediction/determination can be made regarding lymph node metastasis, which is an important phenomenon in cancer progression. | 2015-10-15 |
20150292038 | MICRO-CHIP FOR DIAGNOSIS AND INTEGRATED ROTARY DIAGNOSIS METHOD USING THE SAME - Provided is a micro-chip for diagnosis, including a unit process part located apart from a rotation center, which includes: a target substance capturing unit having a capture passage and a capturing means filling a capture passage; a sample storing unit connected to capture passage and giving an inner space in which a sample is stored; a washing buffer chamber connected to the capture passage and giving an inner space in which a washing buffer is stored; an elution buffer chamber connected to the capture passage and giving an inner space in which an elution buffer is stored; a reaction solution chamber giving a space in which a reaction solution required for a PCR process is stored; a discharge passage connected to the target substance capturing unit and the reaction solution chamber; a wasted solution chamber connected to the discharge passage; and a target substance chamber connected to the discharge passage. | 2015-10-15 |
20150292039 | METHOD TO AMPLIFY NUCLEIC ACIDS OF FUNGI TO GENERATE FLUORESCENCE LABELED FRAGMENTS OF CONSERVED AND ARBITRARY PRODUCTS - Disclosed herein are methods for the identification of the species, serotype, and strain of a fungi. Also disclosed are primers for use in detecting such fungi and kits comprising such primers. | 2015-10-15 |
20150292040 | DEVELOPMENT OF SIMPLE DISCRIMINATION METHOD FOR LOW-QUALITY ES CELLS AND iPS CELLS AS INDICATOR OF BIOLOGICAL CLOCK, AND DEVELOPMENT OF CELL EVALUATION METHOD AS INDICATOR OF BIOLOGICAL CLOCK - A present invention provides a method for evaluating pluripotent stem cells comprising: analyzing expression of a clock gene in cells differentiated from a pluripotent stem cell, and evaluating the pluripotent stem cells based on the degree of the expression. | 2015-10-15 |
20150292041 | METHODS AND COMPOSITIONS FOR IDENTIFYING ENTEROVIRUS - Certain embodiments of the invention include, but are not limited to PCR primer pairs, sequencing primers, and/or associated thermocycling protocols targeting a region identified by the inventors within the 5′ untranslated region of enteroviruses for the purpose of identifying, subtyping, and/or classifying of virus in samples using nucleic acid sequencing. The sequencing procedures can use nucleic acid templates, such as cDNA or PCR amplicons, as a template for sequencing in medium to high throughput format that is cost effective and easily deployed to other clinical microbiology laboratories. | 2015-10-15 |