48th week of 2021 patent applcation highlights part 32 |
Patent application number | Title | Published |
20210371768 | HYDRAULIC FLUIDS HAVING BIODEGRADABLE POLYALKYLENE GLYCOL RHEOLOGY MODIFIERS USEFUL IN SUBSEA APPLICATIONS - A composition comprising water, a glycol, and a polyalkylene glycol which has a biodegradability of at least 60% as determined using OECD 301F wherein the composition has a kinematic viscosity of at least 25 mm | 2021-12-02 |
20210371769 | LUBRICATING OIL COMPOSITION AND METHOD FOR PRODUCING SAME - A method for producing a lubricating oil composition includes: a step of mixing a base oil and fullerene, dissolving a soluble component of the fullerene in the base oil, and obtaining a mixture of the base oil and fullerene; a step of removing an insoluble component included in the mixture and obtaining a fullerene solution; and a step of heat-treating the fullerene solution. | 2021-12-02 |
20210371770 | PROCESS FOR ENZYMATIC OIL DEGUMMING - The present invention relates to a process for reducing an amount of intact phospholipids in a triacylglyceride oil comprising incubating the oil with a polypeptide having phospholipase A1 activity, wherein the polypeptide comprises a polypeptide having at least 80% identity to the mature amino acid sequence of SEQ ID NO: 1. | 2021-12-02 |
20210371771 | METHOD FOR UPGRADING LOW-VALUE AND WASTE FATS, OILS, AND GREASES - The present technology provides a method that includes contacting a composition with a caustic solution to produce a caustic-treated composition; combining the caustic-treated composition with silica particles to produce a slurry; and removing the silica particles from the slurry to produce a treated composition; wherein the composition includes one or more of animal fats, animal oils, plant fats, plant oils, vegetable fats, vegetable oils, greases, and used cooking oil and the composition includes: at least about 10 wppm of total metals, at least about 8 wppm of phosphorus, at least about 10 wppm of chlorine, at least about 10 wppm of sulfur, at least about 20 wppm of nitrogen, at least about 5 wt. % of free fatty acids; and has an acid number from about 10 mg KOH/g to about 150 mg KOH/g, and the silica particles has a particle size from about 10 microns to about 50 microns, a BET surface area from about 200 m | 2021-12-02 |
20210371772 | PROCESS FOR ISOLATION OF STEROLS AND A FRACTION RICH IN FATTY ACIDS AND RESIN ACIDS - The invention relates to a process for recovering sterols and a fraction rich in fatty acids and resin acids from tall oil pitch, said process comprising a) saponifying the tall oil pitch with an alkali to hydrolyse esters included in the pitch to free alcohols and organic acids in salt form, b) acidulating the saponified pitch with a mineral acid to convert the organic acids in salt form into free organic acids and to form an organic phase and an aqueous phase; wherein mineral acid is provided in an amount to produce an aqueous phase with a pH value of at most 3.8, c) separating the aqueous phase with a pH value of at most 3.8 from the organic phase, d) evaporation fractionating the organic phase in a thin film evaporator to obtain a distillate rich in fatty acids and resin acids and a bottom fraction rich in sterols, e) evaporation fractionating the bottom fraction to obtain a distillate rich in sterols, and f) subjecting the sterols in the sterol-rich distillate to crystallisation purification, wherein the process excludes a step of adding alkali between step c) and step d). | 2021-12-02 |
20210371773 | MIXTURE COMPOSITION COMPRISING GLUCOLIPIDS - The invention relates to a mixture composition comprising glucolipids, to its use for producing formulations and to formulations comprising this mixture composition. | 2021-12-02 |
20210371774 | POST-CMP CLEANING COMPOSITION FOR GERMANIUM- CONTAINING SUBSTRATE - A cleaning composition for cleaning a surface of a substrate comprising silicon germanium after a chemical mechanical polishing process is provided. The cleaning composition includes an oligomeric or polymeric polyamine, at least one wetting agent, a pH adjusting agent, and a solvent. | 2021-12-02 |
20210371775 | ACIDIC CLEANING COMPOSITIONS FOR ENHANCED SOIL REMOVAL - Concentrated nitric acid cleaners employing the use of ethoxylated fatty alcohols, including a blend of polyethoxylated fatty alcohols, provide enhanced cleaning on metal and other surfaces, particularly stainless steel. Methods of use, including enhanced fatty soil removal and organic soil removal (such as soap scum) are also disclosed. | 2021-12-02 |
20210371776 | CLEANING SOLUTION FOR REMOVING CERIUM COMPOUND, CLEANING METHOD, AND METHOD FOR PRODUCING SEMICONDUCTOR WAFER - The present invention relates to a cleaning solution for removing a cerium compound, a cleaning method, and a method for producing a semiconductor wafer. An object of the present invention is to provide a cleaning solution having excellent removability of a cerium compound. The cleaning solution for removing a cerium compound of the present invention contains an aminopolycarboxylic acid compound having a coordination number of 7 or more. | 2021-12-02 |
20210371777 | SOLID RINSE AID COMPOSITION COMPRISING POLYACRYLIC ACID - Solid rinse aid compositions, methods of use, and methods of making said composition are disclosed. Rinse aid is provided by a solidification agent, a sheeting agent, a defoamer component, and a polyacrylic acid homopolymer or alkali metal salt thereof forming a solid compositions. Preferred solidification agents include aromatic sulfonates. Preferred sheeting agents include one or more alcohol ethyoxylates. Preferred defoamer components include a polymer compound including one or more ethylene oxide groups. The solid rinse aid compositions are preferably substantially free of sulfate and sulfate-containing compounds. | 2021-12-02 |
20210371778 | COMPOSITION FOR USE IN CLEANING METAL COMPONENTS - A composition for use in cleaning metal components having Hansen Solubility Parameters for the composition of δ | 2021-12-02 |
20210371779 | ALCOHOLIC BEVERAGE - An object of the present invention is to control a stimulation of a tingling aftertaste caused by limonene and alcohol in an alcoholic beverage containing limonene. The present invention provides an alcoholic beverage containing 10 to 60 ppm of limonene and 5 to 25 ppm of eriocitrin. | 2021-12-02 |
20210371780 | Process for Aging Distilled Spirits - The present invention relates to a process for producing distilled spirits. After fermentation and distillation, the freshly distilled spirits are placed in wooden barrels and subjected to an initial aging process. Thereafter, the wooden barrels are placed in a tobacco curing and drying barn and aged therein for a selected period of time. | 2021-12-02 |
20210371781 | Hybrid Solid State-Submerged Fermentation Using a Matrix - The subject invention provides methods and systems for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, agriculture, and many other applications. More specifically, the subject invention provides methods and systems for producing microorganisms and/or growth by-products thereof using a hybrid solid state-submerged fermentation method, wherein a matrix comprised of a solid material covered in an alginate coating is formed inside a liquid fermentation vessel. In some embodiments, the solid material is a plurality of natural loofa sponges. | 2021-12-02 |
20210371782 | METHODS FOR OPTICAL MICROPATTERNING OF HYDROGELS AND USES THEREOF - The present invention provides methods for optically micropatterning hydrogels, which may be used for, e.g., regenerative medicine, synthetic or cultured foods, and in devices suitable for use in high throughput drug screening assays. | 2021-12-02 |
20210371783 | Bioreactor - A bioreactor comprising a housing defining a perfusion chamber, the housing including at least one port, wherein the at least one port is coupled to the housing, and a sample holder positioned within the perfusion chamber. A bioreactor and spheroid-based biofabrication method for making perfusible tissue constructs and perfusing them. | 2021-12-02 |
20210371784 | CELL-CONTAINING VESSEL AND METHOD FOR PRODUCING NEURAL CELL-CONTAINING SPHEROID - The invention provides a cell-containing vessel comprising a plurality of neural cell-containing spheroids for which the variation calculated using the calculation method described below is less than 20%, wherein the neural cell-containing spheroids contain a plurality of types of cells including neural cells, | 2021-12-02 |
20210371785 | Disposable Cell for In Situ Monitoring Probe - An in situ probe, comprising reusable and disposable components, can be employed to measure cell viability in a rocking bioreactor. | 2021-12-02 |
20210371786 | MICROFLUIDIC DEVICES FOR DENTAL APPLICATIONS - Disclosed is a microfluidic device having a channel a chamber in fluid communication with the channel. The chamber is sized to contain dental sample that is form a semipermeable barrier through which fluid from the channel is deliverable to the dental sample. | 2021-12-02 |
20210371787 | SYSTEMS AND METHODS COMPRISING OPEN ALGAE CULTIVATION LINERS - The present disclosure relates to algae cultivation including the integration of liners for large-scale open algal biomass to facilitate algae facility commercial scale-up operations. Liners include those having a first portion having a first thickness and a second portion comprising a second thickness, wherein the first thickness and the second thickness are different. Liners also include those having a first portion having a first thickness, a second portion comprising a second thickness, and a third portion comprising a third thickness, wherein the first thickness, the second thickness, and the third thickness are different. | 2021-12-02 |
20210371788 | APPARATUS AND METHOD FOR CULTURING CELLS - An apparatus and method for preparing and culturing cells comprises a gas permeable chamber having at least an inlet and an outlet for introducing culture medium and cells. A cell growth substrate placed at the end(s) of the gas permeable chamber for cells anchored or embedded. The present invention discloses a simple but efficient cell culture method by slowly rotating the cell culture apparatus horizontally combining with a partially filled culture media in the gas permeable container in order to be able to expose the carriers and submerge the carriers intermittently, to achieve an optimal cell culture environment through the efficient oxygen transfer, CO2 balance, with sufficient nutrient supply, without vigorous agitation to achieve oxygen transfer. The horizontally rotation movement with the present cell growth apparatus efficient the nutrition, carbon dioxide and oxygen transfer during cultured process for production of cellular protein products, viruses or harvesting the cells. | 2021-12-02 |
20210371789 | FIXED BED BIOREACTOR AND METHODS OF USING THE SAME - A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon. | 2021-12-02 |
20210371790 | TISSUE ENGINEERED SCAFFOLDS, INSTRUMENTED BIOREACTORS AND METHODS OF USE THEREOF - Systems and methods are provided including a housing configured to receive and engage a hollow tissue structure within a fluid chamber of the housing. A first pair of flow channels and a second pair of flow channels of the housing are fluidly coupled to the fluid chamber. The housing fluidly couples the first pair of flow channels and fluidly couples the second pair of flow channels via a second flow path such that a change in a fluid pressure differential between a first fluid in the first flow path and a second fluid in the second flow path deflects at least a portion of the hollow tissue structure causing a change in flow of the first fluid through the first pair of flow channels or a change in flow of the second fluid through the second pair of flow channels. | 2021-12-02 |
20210371791 | PACKED-BED BIOREACTOR SYSTEMS AND METHODS OF USING THE SAME - A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform. | 2021-12-02 |
20210371792 | ARTIFICIAL TISSUE PERFUSION DEVICE AND METHOD OF DRUG ASSESSMENT USING ARTIFICIAL TISSUE - An object of the present invention is to provide an artificial tissue perfusion device capable of analyzing the interaction between a vascular cell layer and a parenchymal cell layer with high accuracy. An artificial tissue perfusion device includes a co-culture system (C) in which a plurality of types of cell are cultured. The co-culture system has a tubular well part ( | 2021-12-02 |
20210371793 | CONTINUOUS PERFUSION BIOREACTOR SYSTEM - Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein. | 2021-12-02 |
20210371794 | PHOTOLYTIC BIOREACTOR SYSTEM AND METHOD | 2021-12-02 |
20210371795 | DEVICE FOR THE CULTIVATION OF AND RADIATION-INDUCED KILLING OF CELLS AND METHOD FOR ANALYZING A MIGRATION AND/OR HEALING OF A WOUND - The invention relates to a device designed for the cultivation and radiation-induced killing of living biological cells. The device comprises a flat substrate and a functional layer for creating a wound in biological cells, said functional layer being applied to the flat substrate. The functional layer contains at least one photosensitizer which is designed to convert triplet oxygen into singlet oxygen by the application of electromagnetic radiation. As a result, biological cells on the functional layer can be killed by irradiation of low-intensity electromagnetic radiation. A wound can be introduced into a cell layer at a locally defined point easily, quickly, carefully, and in a flexible and cost-effective manner and thus the healing of the wound can be studied. The invention further relates to uses of the devices and a method for analyzing a migration and/or wound healing behavior of biological cells. | 2021-12-02 |
20210371796 | Control of Cell Electroporation - An electrode unit for performing electroporation of a biological cell is provided, including at least one electrode which can contact a cell. A stimulation unit and an impedance measurement device are connected to the cell, respectively to provide signals to provide cell electroporation and to measure the impedance of the electrode in contact with the cell. Further, a memory stores a predetermined value of an impedance parameter of the biological cell, and it is arranged to be readable by a comparing element. The comparing element is configured to compare the value stored in the memory with a value measured with the impedance measurement device, and to produce an adjustment signal to the stimulation unit, forming a feedback loop. The unit is further configured to apply a further electrical signal for providing electroporation of the cell upon receiving the adjustment signal from the comparing element. | 2021-12-02 |
20210371797 | METHODS AND DEVICES FOR ELECTROPORATION - An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells. | 2021-12-02 |
20210371798 | Monitoring Chamber - A bypass flow monitoring chamber that may be retrofit onto the main chamber of an incubation chamber, for continuous collection of environmental samples. This allows for the measurement and correction of the environmental conditions within the chamber, and the return of the sample to the main chamber. Utilizing this sampling method, the separable bypass flow monitoring chamber may be isolated from the main chamber by a set of valves during a period of high-heat decontamination of the incubator therein protecting the sensors and contents of this separable chamber, as well as allowing the use of less expensive and lower temperature rated incubator sensors. | 2021-12-02 |
20210371799 | DETECTION OF NUCLEASE EDITED SEQUENCES IN AUTOMATED MODULES AND INSTRUMENTS - The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited. | 2021-12-02 |
20210371800 | SYSTEMS AND METHODS FOR THERMAL MANAGEMENT OF ALGAE CULTIVATION SYSTEMS - Systems and methods for algae cultivation and, more particularly, systems and methods for controlling algae cultivation water slurry temperature to optimize algae facility production and facilitation of algae facility commercial deployment in a wide spectrum of environmental locations. Thermal reservoirs comprising temperature-based, phase-transitioning material(s) are integrated with algae cultivation vessels to provide temperature control of cultivating algae slurries. | 2021-12-02 |
20210371801 | CANCER CELL ENRICHMENT SYSTEM - Embodiments of the invention relate to a cell culture incubator having a gas flow regulation system that exerts control over atmospheric parameters within the incubator. Particular embodiments include an enclosed environmental chamber and a control unit operably linked thereto, the control unit having an oxygen module and a pressure module. Control unit embodiments, by way of these modules, are configured to regulate both oxygen partial pressure and total gas pressure within the enclosed environmental chamber. Embodiments of the control unit are adapted (a) to provide instructions to the oxygen module to regulate an oxygen level to an instructed hypoxic oxygen level and (b) to provide instructions to the pressure module to regulate total gas pressure to an instructed positive pressure level. The regulation of oxygen to the instructed hypoxic level prevails despite an oxygen partial pressure-increasing effect of the positive pressure condition associated with the instructed positive pressure level. | 2021-12-02 |
20210371802 | Device and Method for Automated Antibiotic Susceptibility Testing of Gram-Negative Bacteria - An antibiotic susceptibility testing device of gram-negative bacteria, as well as a corresponding method, are discussed. The device has a temperature control unit (including a constant temperature chamber) and a contactless conductivity-based measurement system. Disposable glassy or PVC tubes are used as test vessels for AST. In the performance of AST assay, appropriate kind of liquid medium containing identical amount of target bacterial cells and target antibiotics at different concentrations are loaded into test tubes, following by incubation in the device at a setup temperature. The bacterial growth profile is monitored by collecting the differential values (ΔC) of conductivity of liquid medium, which depend on the proliferation of viable cells. Outcome of ΔC indicates whether the target bacterial cells are completely inhibited by the test antibiotic or not, enabling the user to judge the value of the minimal inhibitory concentration (MIC) simply. | 2021-12-02 |
20210371803 | MULTI-PARAMETER AUTOMATIC BLOOD CELL COUNTING DEVICE - A multi-parameter automatic blood cell counting device includes a basic information generating part, an immune cell subgroup detection part, an immunodynamic change analyzing part, a storage part, and display part. The basic information generating part acquires examination information about a blood count of blood components and a white blood cell image and CD classification analysis information about monoclonal antibodies that bind to lymphocyte surface antigens. The immune cell subgroup detection part detects information about immune cell subgroups required for analyzing an immune status of a subject based on the examination information and the CD classification analysis information. The immunodynamic change analyzing part identifies information about the change in immunodynamics of the subject based on the information detected by the immune cell subgroup detection part and the examination information and CD classification analysis information stored in the storage part, and displays an image of the identified information on the display part. | 2021-12-02 |
20210371804 | Videomicroscopy of contractile cell cultures and cell culture methods using same. - A method for characterizing contractions in cell cultures of contractile cells, the method comprising: acquiring a series of images of the contractile cells; for each image in the series of images, computing a movement index characterizing the rate of change in mean absolute pixel intensity variations across the whole image; and using the movement index to assess the contractions of the contractile cells. Also, applications of the method to the study of cardiac tissue analogs and other cell cultures, and a culture system used to implement the method. | 2021-12-02 |
20210371805 | LIQUID THICKNESS CONTROLLING MECHANISM, CULTURE DEVICE, AND LIQUID THICKNESS CONTROLLING METHOD - A liquid thickness of a medium in a culture bag used in cell culture can be controlled. A liquid thickness controlling mechanism that controls the liquid thickness of the medium filled in the culture bag provided with at least one port and formed of a soft packing material includes a detection portion that measures a change caused by a change in the liquid thickness of the medium and a control portion that controls operation of liquid delivery means disposed in a tubular member connecting the culture bag and a medium bag based on detection information inputted from the detection portion so as to control delivery of the medium between the culture bag and the medium bag. Using the liquid thickness controlling mechanism makes it possible to control the liquid thickness of the medium in the culture bag being used. | 2021-12-02 |
20210371806 | Networked incubator operation - Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well. | 2021-12-02 |
20210371807 | Lactobacillus Gasseri HMV18 and Exocrine Protein and Application Thereof - The present disclosure relates to the technical field of microorganisms, in particular to | 2021-12-02 |
20210371808 | ACHROMOBACTER XYLOSOXIDANS WITH MONOMETHYLAMINE DEGRADABILITY AND APPLICATION THEREOF - The present invention discloses a strain of | 2021-12-02 |
20210371809 | COPPER LOSS MITIGATION - Provided herein is are methods of preventing loss of copper in a solution. The solution is a cell culture medium, cell culture feed or cell culture additive comprising copper (II) and cysteine. The method comprises having substantially the same amount of soluble copper before and after filtering the solution. Also provided are methods of preparing a cell culture medium, cell culture feed or cell culture additive comprising copper (II), cysteine, and other components, without loss of soluble copper. Also provided are methods of culturing a cell or making a biological product comprising use of media prepared according to said methods of preventing loss of copper or preparing said media. Further provided is media prepared according to said methods and use of such media. | 2021-12-02 |
20210371810 | Cell Culture Medium - The present invention relates to a cell culture medium for preparing liver, gastric, pancreatic, colon or intestinal adult stem cell isolated from adult tissue, as well as for maintaining such stem cell in the undifferentiated state. The cell culture medium comprises a base medium; an ABL and SRC dual kinase inhibitor/an ABL kinase inhibitor and a SRC kinase inhibitor; a mitogenic factor; a WNT signalling pathway activator; a stimulator for NAD+ and NADP+ generation; and a cAMP/P KA pathway activator. In a particular embodiment, the ABL and SRC dual kinase inhibitor is Dasatinib; the mitogenic factor is EGF; the WNT signalling pathway activator is R-Spondin 1; the stimulator for NAD+ and NADP+ generation is nicotinamide; and the cAMP/PKA pathway activator is cholera endotoxin. | 2021-12-02 |
20210371811 | pH-MODULATING BIODEGRADABLE POLYMER AND POLY(GLYCEROL SEBACATE)-AUGMENTED CELL CULTURE MEDIA - A pH-modulating poly(glycerol sebacate) composition includes poly(glycerol sebacate) and at least one pH-modulating agent associated with the poly(glycerol sebacate). A process of making a pH-modulating poly(glycerol sebacate) composition includes forming a poly(glycerol sebacate) by a water-mediated reaction from glycerol and sebacic acid and associating at least one pH-modulating agent with the poly(glycerol sebacate). A process of modulating a pH of a buffered aqueous solution includes placing a pH-modulating poly(glycerol sebacate) composition in a buffered aqueous solution. The pH-modulating agent is released into the buffered aqueous solution during degradation of the poly(glycerol sebacate) to reduce a decrease in pH of the buffered aqueous solution caused by degradation of the poly(glycerol sebacate). | 2021-12-02 |
20210371812 | CELL DIFFERENTIATION MEDIUM COMPOSITION, HIGH SECRETION INSULIN-PRODUCING CELLS AND PREPARATION METHOD THEREOF - The present invention relates to a cell differentiation medium composition, a high secretion insulin-producing cells and a preparation method thereof. The high secretion insulin-producing cells obtained by using the cell differentiation medium composition to induce stem cell differentiated under specific conditions can secrete a large amount of insulin in a short time, and when the high-secreting insulin-producing cells are transplanted into the human body, they are not easy to be swallowed by macrophages, which can improve the survival rate of the insulin-producing cells and prolong the time of insulin secretion thereby. | 2021-12-02 |
20210371813 | RAPID CELL ISOLATION AND RECOVERY USING HOLLOW GLASS MICROSPHERES COATED WITH BIODEGRADABLE NANOSTRUCTURED FILMS - Hollow glass microspheres (HGMS) with a controlled nanotopographical surface structure ( | 2021-12-02 |
20210371814 | USE OF CISTANCHE DESERTICOLA POLYSACCHARIDES IN PROMOTING PROLIFERATION AND DIFFERENTIATION OF FEMALE GERMLINE STEM CELLS | 2021-12-02 |
20210371815 | COMPOSITIONS AND METHODS FOR OBTAINING VASCULARIZED HUMAN INTESTINAL ORGANOID TISSUE, AND RELATED USES THEREOF - The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting vascularized human intestinal organoid tissue having an intestine-specific EC transcriptional signature from hindgut spheroid tissue produced in vitro from the described methods. | 2021-12-02 |
20210371816 | METHOD OF PRODUCING CELL-CONTAINING CONTAINER - A method of producing a cell-containing container is provided, including a process in which pluripotent stem cells that have been induced to differentiate into neurons, and astrocytes are mixed and seeded in a cell culture container in which an electrode array is arranged on a culture surface, and a process in which the cell culture container is incubated, and as a result, the pluripotent stem cells and the astrocytes adhere to the culture surface, and the pluripotent stem cells differentiate into neurons. | 2021-12-02 |
20210371817 | COMPOSITIONS AND METHODS FOR PRECISE PATTERNING OF POSTERIOR NEUROECTODERM FROM HUMAN PLURIPOTENT STEM CELLS - Described herein are methods, compositions, and kits for directed differentiation of human pluripotent stem cells into caudal lateral epiblasts, posterior neuroectoderm or posterior neuroepithelium, or motor neurons having specified HOX gene expression pattern mirroring a desired position along the rostral-caudal axis during hindbrain and spinal cord development. Also described are isolated populations of cells including caudal lateral epiblasts, posterior neuroectoderm, posterior neuroepithelium, or motor neurons having a HOX gene expression pattern specified to correspond to the HOX gene expression pattern associated with a desired rostral-caudal axis position. | 2021-12-02 |
20210371818 | METHOD FOR INDUCING DIFFERENTIATION OF CORNEAL EPITHELIAL CELLS FROM PLURIPOTENT STEM CELLS - The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells. | 2021-12-02 |
20210371819 | Method for Generating Human Multipotent Mammary Stem Cells From Normal Primary Breast Luminal Cells - The invention relates to methods for generating multipotent mammary stern cells from isolated and cultured human breast luminal cells. The method comprises the steps: 1. isolating and growing normal differentiated cells in vitro; 2. treating the differentiated cells with either a conditioned medium from active fibroblasts or cytokine. The invention also relates to multipotent mammary stem cells, cultures of the multipotent stem cells, differentiated cells, tissues, organs derived from the culture multipotent stem cells isolated by the methods disclosed and therapeutic and other uses for those cells thereof. | 2021-12-02 |
20210371820 | METHOD FOR AMPLIFYING ANTIGEN-SPECIFIC REGULATORY T CELLS IN VITRO - The invention discloses a method for amplifying antigen-specific regulatory T cells in vitro, belonging to the technical field of biomedicine. The invention adopts Rapamycin combined with TGF-β cells to induce human T cells into antigen-specific regulatory T cells with immunosuppressive function in vitro by the action of DC cells, which has the advantages as follows: 1) the amount is sufficient; 2) the obtained regulatory T cells can resist differentiation to Th17 cells; 3) the obtained regulatory T cells have stronger inhibitory functions and biological effects compared with the regulatory T cells induced by other methods. The invention overcomes many defects of natural regulatory T cells and has greater therapeutic advantages in the treatment of inflammatory diseases, autoimmune diseases and prevention of organ transplant immunological rejection. | 2021-12-02 |
20210371821 | INDUCED REGULATORY T CELLS, METHODS OF PRODUCTION, AND USES THEREOF - The present disclosure provides methods for producing cell populations enriched for induced regulatory T cells (iTregs). In particular, the disclosure relates to methods for culturing T cells such that the final culture is enriched for induced regulatory T cells. The disclosure also relates to methods for inducing regulatory T cells. Also provided are compositions enriched for induced regulatory T cells, which are useful for treating individuals in need of such treatment. The methods and compositions disclosed herein can also be used to treat individuals suffering from immune-mediated diseases. | 2021-12-02 |
20210371822 | METHODS FOR EXPANDING SARS-COV2-ANTIGEN-SPECIFIC T CELLS, COMPOSITIONS AND USES RELATED THERETO - Provided herein are methods for preparing and characterizing SARS-co2 antigen specific immune cell cultures and preparations and methods of using the same in adoptive immunotherapy for cancer, infections and immune disorders. Also, provided are compositions and methods for generating immune cells expressing synthetic antigen binding receptors targeting SARS-cov2 and methods of use of these cells for the treatment and prevention of COVID-19. Also provided are compositions and methods for determining immune response to SARs-cov2 in a subject, detecting SARS-cov2, measuring cytotoxicity induced by SARS-cov2, and detecting the expression and cytotoxicity of synthetic antigen binding receptors targeting SARS-cov2. | 2021-12-02 |
20210371823 | METHOD FOR EXPANDING HUMAN DC CELL AND HUMAN DC CELL RESOURCE LIBRARY - Provided is a method for expanding a human DC cell. The method includes the step of contacting a cell sample of a DC cell to be expanded with a viral transactivator protein sourcing from simian-T-lymphotropic virus (STLV). Also provided are an expanded DC cell prepared by the method, and a DC cell and data repository constructed by the method. | 2021-12-02 |
20210371824 | PRODUCTION OF MEGAKARYOCYTES IN BIOREACTORS - An in vitro process for producing megakaryocytes, and optionally platelets from the megakaryocytes, including the steps of cultivating stem cells, e.g. induced pluripotent stem cells, to generate aggregated pluripotent stem cells, preferably cultivating the aggregates in suspension in medium, inducing differentiation in these aggregates, and isolating megakaryocytes from the culture medium. | 2021-12-02 |
20210371825 | COMPOSITIONS FOR AND METHODS OF PRODUCING TUMOR ORGANOIDS - Provided herein are methods of enhancing the production of organoids from primary tissues, which can be either normal or cancerous, by culturing the primary tissues in the presence of collagen I homotrimers. To this end, also provided are cell culture media comprising exogenous collagen I homotrimers. Also provided are organoids produced by the disclosed methods as well as methods of using the organoids so produced. | 2021-12-02 |
20210371826 | Renal Progenitor Cells - A method is provided for simultaneously producing both nephron progenitor cells and ureteric epithelial progenitor cells including the step of contacting intermediate mesoderm cells with: fibroblast growth factor 9 and/or fibroblast growth factor 20 and optionally, one or more selected from the group consisting of: bone morphogenic protein 7; heparin; a Wnt agonist; retinoic acid; and an RA antagonist. The concentrations of Wnt agonist, retinoic acid and/or RA antagonist may be manipulated to favour the relative production of nephron progenitor cells and ureteric epithelial progenitor cells. The intermediate mesoderm cells are ultimately derived from human pluripotent stem cells via a posterior primitive streak stage. The nephron progenitor cells and ureteric epithelial progenitor cells may have end uses such as for kidney repair and regeneration, bioprinting of kidneys and screening compounds for nephrotoxicity. | 2021-12-02 |
20210371827 | METHOD FOR PREPARING CANCER STEM CELL SPHEROIDS - The present invention relates to a method or kit for producing cancer stem cell spheroids, and a method of screening of drugs for treating cancer cell resistance using the prepared cancer stem cell spheroid, and it can conveniently produce cancer stem cell spheroids, and the prepared cancer stem cell spheroids can be effectively utilized for screening drugs for treating cancer cell resistance. | 2021-12-02 |
20210371828 | INDUCED PLURIPOTENT CELL COMPRISING A CONTROLLABLE TRANSGENE FOR CONDITIONAL IMMORTALIZATION - The invention relates to induced pluripotent stem cells that are generated from cells, for example Adult Stem Cells, that are conditionally-immortalisable. In particular, the invention relates to induced pluripotent stem cells generated from stem cell lines comprising a controllable transgene for conditional immortalisation, and the progeny of those induced pluripotent stem cells. Induced pluripotent stem cells, progeny cells derived from those pluripotent cells, compositions comprising those cells, methods of making all of those cells, and uses of all of those cells are also described. | 2021-12-02 |
20210371829 | ENGINEERED KETOREDUCTASE POLYPEPTIDES AND USES THEREOF - Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions. The use of the engineered polypeptides of the present invention greatly simplifies the production process of chiral alcohol compounds, reduces the cost of production and the impact on the environment, and has good industrial application prospects. | 2021-12-02 |
20210371830 | NOVEL SALT-LOVING BACILLUS POLYFERMENTICUS STRAIN FOR PRODUCING SALT-TOLERANT GAMMA-GLUTAMYL TRANSPEPTIDASE - The present invention relates to a halophilic | 2021-12-02 |
20210371831 | STEVIOL GLYCOSYLTRANSFERASES AND GENES ENCODING THE SAME - Polynucleotides encoding corresponding polypeptides capable of glycosylating steviol at its C-19 position to produce a steviol glycoside, an expression vector including such a polynucleotide, a method for producing a steviol glycoside by culturing a recombinant host cell containing such an expression vector under conditions in which the cell expresses the UDP-glycosyltransferase from the polynucleotide, and a method for producing a steviol glycoside by contacting a composition including steviol with a recombinant UDP-glycosyltransferase. The steviol glycoside can be steviol-19-O-glycoside. The recombinant host cell containing such an expression vector can be a bacterial cell, a plant cell, or a fungal cell, an animal cell, or a multicellular organism such as a plant. | 2021-12-02 |
20210371832 | COMPOSITIONS FOR INHIBITING KRAS SIGNALING AND METHODS OF MAKING AND USING SAME - In some aspects, provided herein are computational methods for structure-based protein design. These new methods and incorporated algorithms speed-up computational structure-based protein design while maintaining accurate calculations, allowing for larger, previously infeasible protein designs. In another aspect, provided herein are mutant c-RAF proteins, and conjugates comprising the same. The conjugates may be used in methods of treating cancer in a subject, such as in methods of treating a KRas mutant cancer. | 2021-12-02 |
20210371833 | POLYMERASES - Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription. | 2021-12-02 |
20210371834 | POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME - The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties. | 2021-12-02 |
20210371835 | MEANS AND METHODS FOR CLEAVAGE OF ZEARALENONE - The present invention relates to a method for increasing the stability of an α/β-hydrolase. In addition, the present invention relates to an α/β-hydrolase obtainable by the method of the present invention. Also provided are α/β-hydrolases having a decreased grand average of hydropathy (GRAVY) value and/or comprising specific mutations. In addition, the present invention concerns a use of an α/β-hydrolase of the present invention for degrading zearalenone (ZEN). | 2021-12-02 |
20210371836 | Methods And Compositions Related To Thioesterase Enzymes - The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels. | 2021-12-02 |
20210371837 | OPTIMIZED FIG4 GENES AND EXPRESSION CASSETTES AND THEIR USE - This invention relates to polynucleotides comprising optimized FIG4 open reading frame (ORF) sequences, vectors comprising the same, and methods of using the same for delivery of the ORF to a cell or a subject and to treat disorders associated with aberrant expression of a FIG4 gene or aberrant activity of a FIG4 gene product in the subject, such as CMT4J. | 2021-12-02 |
20210371838 | NON-STANDARD AMINO ACID CONTAINING COMPOSITIONS AND USES THEREOF - Provided herein is a stabilized DNase I polypeptide containing a non-standard amino acid that maintains enzymatic activity even under harsh conditions, such as reducing environments. The stabilized DNase I polypeptide has enzymatic activity in reducing environments that is higher than a corresponding DNase I polypeptide without the non-standard amino acids under the same conditions. Also provided herein are polynucleotides encoding the stabilized DNase I polypeptide, cells for expressing and/or producing the stabilized DNase I polypeptide, and methods of use of the stabilized DNase I polypeptide. | 2021-12-02 |
20210371839 | Products and Methods for Assessing and Increasing Klotho Protein Levels - Disclosed are products and methods for monitoring Klotho protein levels and for stabilizing Klotho protein in a mammalian blood sample, especially at room temperature or without freezing, for a period of time. Methods of detecting and quantifying Klotho protein levels, particularly endogenous and/or exogenous soluble alpha Klotho protein levels, methods of diagnosing Klotho protein deficiency, and methods of increasing Klotho protein levels or production, particularly endogenous and/or exogenous soluble alpha Klotho protein level(s), expression, or production, in a mammalian subject, and products useful in performing the same, including diagnostic kits and compositions for treating Klotho protein deficiency, are disclosed. Compositions are configured or formulated to augment natural soluble alpha Klotho protein production, attenuate Klotho protein damage or degradation, and/or supplement Klotho protein levels with exogenous, recombinant protein. Treatment methods and uses include administration of the compositions to human or non-human mammalian subjects. | 2021-12-02 |
20210371840 | Fusion Protein - The invention relates to fusion proteins having depilling, antipilling and/or antigreying performance, and that have improved stability in the presence of proteases. The fusion proteins comprise a cellulase component, a linker component, and a carbohydrate binding component. | 2021-12-02 |
20210371841 | SOLUBLE ACE2 VARIANTS AND USES THEREFOR - The present disclosure provides various insights relating to treatment of viral infection with soluble ACE2 variants, specifically including soluble human ACE2 (hACE2) variants. For example, the present disclosure teaches that decoy activity, which may intercept virus-receptor interactions, and ACE2 enzymatic activity can provide separate contributions to therapeutic efficacy; among other things, the present disclosure provides particular therapeutic regimens (e.g., relating to treatment of particular patient populations and/or to dosing regimens, combination therapies, etc.) for certain soluble ACE2 variants. Still further, the present disclosure provides certain particular ACE2 variants and methods of making and/or using them, including in accordance with particular therapeutic regimens. | 2021-12-02 |
20210371842 | ANTIDOTES TO FACTOR XA INHIBITORS - The present disclosure relates to antidotes to anticoagulants targeting factor Xa. The antidotes are factor Xa protein derivatives that bind to the factor Xa inhibitors thereby substantially neutralizing them but do not assemble into the prothrombinase complex. In one embodiment, the derivatives described herein lack or have reduced intrinsic coagulant activity. | 2021-12-02 |
20210371843 | Genetically Engineered Bacteria, Its Construction Method And Its Application In Producing Nad+ Method - The invention discloses a genetically engineered bacterium in which the gene encoding adenine deaminase on the genome of the bacterium is knocked out or/and the gene encoding the enzyme in the NAD | 2021-12-02 |
20210371844 | NEW MUTANT PROTEIN FOR IMPROVING MALIC ACID YIELD - The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield. | 2021-12-02 |
20210371845 | Synthesis Of Enzyme-Polymer Conjugates, Having Enhanced Activity & Stability - A process for site-specific immobilization of an enzyme on a polymer involving pairing of an enzyme and polymer to optimize cross-linking between the enzyme and the polymer while avoiding conformational or biochemical inhibition of enzyme activity. The process involves site specific interaction in a multiple phase synthesis within a buffered reaction medium and in an inert atmosphere. The reaction kinetics of the conjugation are modulated by chilling the reaction medium containing the enzyme and polymer to about the maximum solvent density of the reaction medium. The cross-linking of the enzyme and a polymer pair performed produces an enzymatically active, stable conjugate. | 2021-12-02 |
20210371846 | CURRENT-BASED STIMULATORS FOR ELECTROGENIC CELLS AND RELATED METHODS - Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower. | 2021-12-02 |
20210371847 | Gapped And Tunable Repeat Units For Use In Genome Editing And Gene Regulation Compositions - Provided herein are DNA binding domains comprising a plurality of repeat units, wherein each repeat unit is expanded or contracted in length. Also provided herein are DNA binding domains comprising a plurality of repeat units, wherein each repeat unit is separated from a neighboring repeat unit by a linker. In certain aspects, the linker includes a recognition site. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a TALE protein. The TALE protein may be a | 2021-12-02 |
20210371848 | COMPOSITIONS FOR HEMOLYSIS AND COAGULATION REGULATION AND STABILIZATION OF EXTRACELLULAR VESICLES - A composition and method of use thereof wherein the composition comprises one or more components capable of releasing an aldehyde, one or more anticoagulants or chelating agents, and one or more polysaccharides. The composition has a pH of from about 4 to about 6. | 2021-12-02 |
20210371849 | INHIBITORY T CELL RECEPTOR PEPTIDES AND DISCOVERY METHODS - Disclosed herein are methods of inhibiting an interaction of a T cell receptor with a peptide-major histocompatibility complex comprising administering inhibitory peptides that bind to the T cell receptor without the aid of a major histocompatibility complex to inhibit the interaction, and methods of identifying the inhibitory peptides. | 2021-12-02 |
20210371850 | ISOLATION AND IMMOBILIZATION OF NUCLEIC ACIDS AND USES THEREOF - Disclosed is a novel technique of directly isolating nucleic acids from a biological sample and use of the isolated nucleic acid complexes for various applications and assays such as biobanking and sequencing. | 2021-12-02 |
20210371851 | SYSTEMS AND METHODS FOR MASSIVELY PARALLEL COMBINATORIAL ANALYSIS OF SINGLE CELLS - Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery. | 2021-12-02 |
20210371852 | SYSTEM OF CELL EXPANSION AND METHODS OF USING THE SAME - The present disclosure relates, at least in part, to a closed and semi-automated system for the isolation of naive T cells, their expansion, and/or final harvest. The disclosure also relates to using those isolated cells in a large batch format for compiling stocks of stimulated CD | 2021-12-02 |
20210371853 | METHODS FOR NUCLEIC ACID LIBRARY CREATION - Provided herein are methods of making nucleic acid libraries. Libraries can be enriched for target nucleic acids by subtractive purification involving the use of poly-tagged capture probes and/or a DNA degradation step performed after a subtractive purification step. | 2021-12-02 |
20210371854 | COMPOSITION FOR MODIFYING TARGET GENE - The present invention provides a delivery technique for delivering a gene modification tool capable of providing a high gene modification efficiency in cells. The composition according to the present invention is a composition for inducing gene modification at a target gene locus in a cell, the composition containing 1) a compound represented by formula (I) or a salt thereof; 2) a structural lipid; and 3) a guide RNA or a DNA including a sequence encoding the guide RNA, and/or an RNA-guided nuclease or a nucleic acid including a sequence encoding the RNA-guided nuclease. In formula (I), n represents an integer of 2 to 5, R represents a linear C | 2021-12-02 |
20210371855 | METHODS OF INHIBITING PROLIFERATIVE CELLS - The present invention provides methods of negatively modulating the Werner protein (WRN) to inhibit proliferative cells characterized by high microsatellite instability (MSI-H), for example to treat proliferative diseases (such as cancer) characterized by high MSI (MSI-H). Further provided are compositions used in such methods. | 2021-12-02 |
20210371856 | MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR THE ASSEMBLY AND EDITING OF MULTIPLE DNA CONSTRUCTS FOR MULTIPLE HOSTS - Methods, compositions, and kits for high throughput DNA assembly reactions in vitro. Modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. | 2021-12-02 |
20210371857 | COMPOSITIONS FOR GENOME EDITING - The present invention relates to compositions and methods for increasing the rate of site specific insertion of a donor DNA sequence to the genome. More specifically, the method introduces a donor DNA template containing at least one transcription factor binding site to a cell in order to favor specific insertion of a donor template sequence at a target site by homology directed repair (HDR). | 2021-12-02 |
20210371858 | METHODS OF SUPPRESSING PATHOGENIC MUTATIONS USING PROGRAMMABLE BASE EDITOR SYSTEMS - Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences. | 2021-12-02 |
20210371859 | RNA MEDIATED GENE REGULATING METHODS - The invention provides methods for the assembly of repeated sequences that are useful in constructing nucleic acids for the simultaneous regulation and editing of multiple genes, and for DNA/RNA origami. | 2021-12-02 |
20210371860 | OLIGONUCLEOTIDES FOR MODULATING MYH7 EXPRESSION - The present invention relates to antisense oligonucleotides that are capable of modulating expression of MYH7 in a target cell. The oligonucleotides hybridize to MYH7 mRNA. The present invention further relates to conjugates of the oligonucleotide and pharmaceutical compositions and methods for treatment of hypertrophic cardiomyopathy using the oligonucleotide. | 2021-12-02 |
20210371861 | Multi-Targeting Nucleic Acid Constructs Composed Of Multiple Oligonucleotides That Modulate Gene Expression Through Complimentary Interactions With Targets - The present invention provides a multi-targeting nucleic acid construct comprising at least: (a) a first nucleic acid portion that is at least partially complementary to at least a first portion of RNA transcribed from a target gene; (b) a second nucleic acid portion that is at least partially complementary to at least a second portion of RNA transcribed from a target gene, which target gene may be the same or different to the target gene defined in (a); (c) a third nucleic acid portion that is at least partially complementary to the first nucleic acid portion of (a), so as to form a first nucleic acid duplex region therewith; (d) a fourth nucleic acid portion that is at least partially complementary to said second nucleic acid portion of (b), so as to form a second nucleic acid duplex region therewith. The construct is designed so that subsequent to in vivo administration the construct disassembles to yield at least first and second discrete nucleic acid targeting molecules that respectively target RNA transcribed from the target genes of (a) and (b). Typically, the first nucleic acid targeting molecule is capable of modulating expression of the target gene of (a), and comprises, or is derived from, at least the first nucleic acid portion of (a). Typically, the second nucleic acid targeting molecule is capable of modulating expression of said target gene of (b), and comprises, or is derived from, the second nucleic acid portion of (b). | 2021-12-02 |
20210371862 | METHOD AND MEANS TO DELIVER miRNA TO TARGET CELLS - The invention relates to the field of gene therapy. In addition the invention relates to the field of interfering RNA and/or microRNA (miRNA). In particular the invention relates to gene therapy involving such miRNA's and more in particular to methods and means to improve delivery of said miRNAs to target cells of a patient. The invention provides for a gene delivery vehicle for use in delivery of a miRNA to a cell resulting in silencing of a desired gene and whereby spread of said miRNA to other non-transduced cells results in silencing of said desired gene in said non-transduced cells. | 2021-12-02 |
20210371863 | HIGH EFFICIENCY APTAMER COMPLEX COMPRISING BRANCHED DNA AND APTAMER, AND USE THEREOF - The present invention relates to a highly efficient aptamer complex comprising a branched DNA and an aptamer, and a pharmaceutical use thereof. More specifically, the aptamer complex of the present invention relates to a highly efficient aptamer complex including a Y-shaped DNA as the branched DNA and using vascular endothelial growth factor (VEGF) as a target molecule. The aptamer complex of the present invention and a pharmaceutical composition comprising the same as an active ingredient are expected to be widely used in the medical field since the binding efficiency with the target molecule is more remarkable than that of the conventional aptamer. | 2021-12-02 |
20210371864 | ASTROCYTE-SPECIFIC NUCLEIC ACID APTAMER AND USE THEREOF - The present specification relates to an astrocyte-specific nucleic acid aptamer and a use thereof. The astrocyte-specific nucleic acid aptamer, according to the disclosure in the present specification, is a DNA aptamer. | 2021-12-02 |
20210371865 | TLR9 AGONISTS FOR USE IN DOWNREGULATING CLEVER-I EXPRESSION ON ALTERNATIVELY ACTIVATED MACROPHAGES - The invention relates to use of TLR9 antagonist in a method for treating cancer and/or chronic infection by downregulating Clever-1 expression on alternatively activated immunosuppressive macrophages. | 2021-12-02 |
20210371866 | METHODS AND COMPOSITIONS FOR TREATMENT OF CHOLESTERYL ESTER STORAGE DISEASE - Provided herein are methods and compositions for treating a subject in need thereof, such as a subject with deficient LAL protein expression or a subject having Cholesteryl Ester Storage Disease. | 2021-12-02 |
20210371867 | ALKALINE PHOSPHATASE FUSION ANTIBODY AND METHOD FOR PRODUCING THE SAME, AND IMMUNOASSAY METHOD - Disclosed is a method for producing an alkaline phosphatase fusion antibody, comprising: culturing a cell comprising an expression vector comprising a gene encoding alkaline phosphatase derived from bovine small intestine or | 2021-12-02 |