51st week of 2012 patent applcation highlights part 44 |
Patent application number | Title | Published |
20120321996 | CATALYST CARRYING FINE METAL PARTICLES AND USE THEREOF - The production method according to the present invention includes a process for producing fine particles formed of a non-precious metal; a process for forming a shell of a precious metal on the respective surfaces of the fine particles of the non-precious metal; and a process for collecting a catalyst from a fluid reaction mixture. A fine metal particle-carrying catalyst prepared by such a production method includes fine non-precious metal particles as the cores thereby reducing the usage of a precious metal to achieve suppression of a cost increase. Since it includes a shell portion formed of a precious metal, it exhibits excellent catalytic activity. | 2012-12-20 |
20120321997 | URETHANES USED AS ADDITIVES IN A PHOTOPOLYMER FORMULATION - The invention relates to a photopolymer formulation comprising matrix polymers, writing monomers and photo initiators, to a method for producing said photopolymer formulation, a photopolymer formulation obtained according to said method, a sheeting, a film, a layer, a layer structure or a moulded body made from said photopolymer formulation and to the use of said photopolymer formulation for producing optical elements, in particular for producing holographic elements and images. | 2012-12-20 |
20120321998 | PHOTOPOLYMER FORMULATION HAVING TRIAZINE-BASED WRITING MONOMERS - The invention relates to a photopolymer formulation comprising polyurethane matrix polymers, writing monomers, and photoinitiators, wherein the writing monomers comprise compounds of formula (I), where R1, R2, R3 independent of each other are each a halogen atom or an organic radical, wherein at least one of the radicals is an organic radical comprising a radiation hardening group. The invention further relates to the use of the photopolymer formulation for producing holographic media. | 2012-12-20 |
20120321999 | GLASS SUBSTRATE-HOLDING TOOL AND METHOD FOR PRODUCING AN EUV MASK BLANK BY EMPLOYING THE SAME - A glass substrate-holding tool, adapted to be employed during the production of a reflective mask blank for EUV lithography (EUVL), includes an electrostatic chuck and a supporting member. The chuck attracts a rear surface of a glass substrate in a non-contact manner by electrostatic attractive force. The supporting member partly supports the rear surface. An area ratio of a projected area of an active surface of the chuck for providing the electrostatic force to an area of a quality-guaranteed region of the rear surface is from 0.5 to 1.0. The active surface is apart from the rear surface by more than 20 μm. The supporting member is configured to support only a region including at least two of four sides defining an outer portion outside the quality-guaranteed region. | 2012-12-20 |
20120322000 | REFLECTIVE MASK BLANK FOR EUV LITHOGRAPHY AND PROCESS FOR PRODUCING THE SAME - To provide an EUV mask blank provided with a low reflective layer, which has excellent properties as an EUV mask blank. A reflective mask blank for EUV lithography comprising a substrate, and a reflective layer for reflecting EUV light, an absorber layer for absorbing EUV light and a low reflective layer to an inspection light (wavelength: 190 to 260 nm) for a mask pattern, formed in this order on the substrate, wherein the low reflective layer has a stacked structure having a first layer containing at least 95 at % in total of silicon (Si) and nitrogen (N), and a second layer containing at least 95 at % in total of tantalum (Ta), oxygen (O) and nitrogen (N) or a second layer containing at least 95 at % in total of tantalum (Ta) and nitrogen (N), stacked in this order from the absorber layer side. | 2012-12-20 |
20120322001 | DEVELOPING AGENT AND IMAGE FORMING APPARATUS - According to one embodiment, a developing agent includes a toner core particle containing a binder resin having a carboxyl group and a coloring agent and a surface layer formed on the toner core particle. The surface layer is formed by crosslinking the toner core particle, a water-soluble polymeric crosslinking agent which crosslinks with a carboxyl group and a water-soluble polymer having a carboxyl group in an aqueous medium. The developing agent can be fixed by a fixing device having a release oil applied thereto. | 2012-12-20 |
20120322002 | Toner Additive Comprising Carbon-Silica Dual Phase Particles - The invention provides a toner composition comprising resin particles, a colorant, and a toner additive, wherein the toner additive comprises carbon-silica dual phase particles, wherein the carbon-silica dual phase particles comprise aggregates of carbon black comprising at least one silicon-containing region, and wherein the carbon-silica dual phase particles are distributed on the surface of the resin particles. The invention also provides a method of preparing the aforesaid toner composition. | 2012-12-20 |
20120322003 | NATURAL OIL-BASED CHEMICALLY PRODUCED TONER - A natural oil-based toner resin for producing toner in developing electrophotographic image comprising a copolymer of unsaturated polyester resins and vinyl monomers, wherein the polyester resins have a molecular weight of 500 to 30000 and obtained from polycondensation between a polycarboxylic acid, a natural oil or derivative thereof and a polyol. The toner resin in emulsion form is mixed with fine suspensions or emulsions of a colourant and a charge control agent; and a flocculating agent is incorporated therein to induce aggregation for forming particles of desired sizes, and subsequently the temperature is suitably raised to cause coalescence to produce raw toner particles of regular shape and smooth surface. The raw toner particles are washed and dried, and treated with suitable additives to produce the finished product of chemically produced toner. | 2012-12-20 |
20120322004 | ELECTROSTATIC CHARGE IMAGE DEVELOPING CARRIER, METHOD OF PRODUCING ELECTROSTATIC CHARGE IMAGE DEVELOPING CARRIER, ELECTROSTATIC CHARGE IMAGE DEVELOPING DEVELOPER, PROCESS CARTRIDGE, IMAGE FORMING APPARATUS, AND IMAGE FORMING METHOD - An electrostatic charge image developing carrier includes a core particle and a coating layer with which the surface of the core particle is coated. The coating layer includes an acrylic resin having a constituent unit in which a silicone chain is disposed in a branch. | 2012-12-20 |
20120322005 | PARTICLES AND METHOD FOR PRODUCING THE SAME, TONER AND METHOD FOR PRODUCING THE SAME, DEVELOPER, PROCESS CARTRIDGE, IMAGE FORMING METHOD AND IMAGE FORMING APPARATUS - A method for producing particles, including: bringing a compressive fluid into contact with a pressure plastic material, so as to plasticize the pressure plastic material; applying a shear force to the compressive fluid and the plasticized pressure plastic material, between which an interface exists, in the presence of a surfactant to granulate the pressure plastic material in the compressive fluid, so as to produce particles. | 2012-12-20 |
20120322006 | Fluorine-Containing Sulfonate Resin, Resist Composition and Pattern Formation Method - A sulfonate resin according to the present invention has a repeating unit of the following general formula (3): | 2012-12-20 |
20120322007 | PATTERN FORMING METHOD, CHEMICAL AMPLIFICATION RESIST COMPOSITION AND RESIST FILM - A pattern forming method comprising (i) a step of forming a film from a chemical amplification resist composition, (ii) a step of exposing the film, and (iii) a step of developing the exposed film by using a developer containing an organic solvent, wherein the resist composition contains (A) a resin, (B) a compound capable of generating a specific acid upon irradiation with an actinic ray or radiation, (C) a crosslinking agent, and (D) a solvent. | 2012-12-20 |
20120322008 | DEVELOPMENT OF PRINTING MEMBERS HAVING POST-ANODICALLY TREATED SUBSTRATES - Gum solutions are formulated to protect a negative-working photopolymer imaging layer coated on an anodized aluminum substrate that has undergone a post-anodic sealing treatment with inorganic phosphate and inorganic fluoride, followed by development. In various embodiments, the developer and gum solution have different compositions but contain at least one polycarboxylic acid—which may be a polymer—that beneficially desensitizes the surface after the unexposed photopolymer layer is removed. | 2012-12-20 |
20120322009 | METHOD OF PREPARING LITHOGRAPHIC PRINTING PLATE AND DEVELOPER FOR LITHOGRAPHIC PRINTING PLATE PRECURSOR - By a method of preparing a lithographic printing plate having exposing a lithographic printing plate precursor including a photosensitive layer containing (A) a polymerization initiator, (B) a polymerizable compound, (C) a sensitizing dye and (D) a binder polymer and a protective layer in this order on a hydrophilic support with laser and then removing the protective layer and an unexposed area of the photosensitive layer in the presence of a developer, in which the developer is a developer which has pH of from 2 to less than 10 and contains an amphoteric surfactant and a nonionic surfactant having an alkylene oxide chain, a simple processing of one solution and one step which does not require a water washing step becomes possible, excellent development property is achieved and a lithographic printing plate which has good printing durability and does not cause printing stain can be provided. | 2012-12-20 |
20120322010 | Novel polymer composition and method of making the same - A novel novolac prepared by acid catalyzed condensation between biphenols or bisphenofluorenes and fluorenone is presented. The polymers exhibit excellent oxidative thermal stability and high carbon content, suitable for dielectric, etch stop applications as spin-on material. | 2012-12-20 |
20120322011 | METHODS AND APPARATUS FOR PERFORMING MULTIPLE PHOTORESIST LAYER DEVELOPMENT AND ETCHING PROCESSES - The present invention provides methods and an apparatus controlling and minimizing process defects in a development process, and modifying line width roughness (LWR) of a photoresist layer after the development process, and maintaining good profile control during subsequent etching processes. In one embodiment, a method for forming features on a substrate includes developing and removing exposed areas in the photosensitive layer disposed on the substrate in the electron processing chamber by predominantly using electrons, removing contaminants from the substrate by predominantly using electrons, and etching the non-photosensitive polymer layer exposed by the developed photosensitive layer in the electron processing chamber by predominantly using electrons. | 2012-12-20 |
20120322012 | COMBUSTION APPLIANCE FOR RAISING THE TEMPERATURE OF EXHAUST GAS - Injection nozzle | 2012-12-20 |
20120322013 | Combustor Housing for Combustion of Low-BTU Fuel Gases and Methods of Making and Using the Same - A combustor housing includes an inlet cover plate having a central inlet configured to receive a supply of one of a high BTU content fuel or air and at least one radially-spaced, peripheral fuel inlet configured to receive a supply of a low BTU content fuel and an outlet cover plate having at least one radially-spaced, peripheral fuel outlet. The combustor housing also includes a peripheral sidewall joining the inlet cover and the outlet cover and enclosing a plenum, the at least one peripheral fuel inlet opening through the inlet cover plate into the plenum and the at least one fuel outlet opening from the plenum through the outlet cover plate. The central inlet opens into at least one conduit which extends away from the central inlet and opens into at least one high BTU content fuel conduit or air supply conduit that is axially aligned with the at least one fuel outlet. | 2012-12-20 |
20120322014 | APPARATUS AND METHOD FOR TREATING A SUBSTRATE - An apparatus for treating a substrate includes a heating member including a heating block, a front rail disposed at a first side of the heating block and configured to support a first edge of the substrate, a rear rail disposed at a second side of the heating block, opposing the first side, and a rail driver configured to move at least one of the front rail and the rear rail. The heating block is configured to receive and heat the substrate, the rear rail is substantially parallel with the front rail and is configured to support a second edge of the substrate, and a distance between the front rail and the rear rail is adjusted to be substantially equal to a width of the substrate. | 2012-12-20 |
20120322015 | APPARATUS AND METHOD FOR TREATING SUBSTRATE - Provided are an apparatus and method for treating a substrate, and more particularly, to a substrate treatment apparatus having a cluster structure and a substrate treatment method using the same. The apparatus for treating the substrate includes a load port on which a container for receiving the substrate is placed, a treatment module for treating the substrate, and a transfer module including a robot for transferring the substrate between the container and the treatment module. The treatment module includes a transfer chamber including a robot for transferring the substrate, a load lock chamber disposed between the transfer chamber and the transfer module, a first treatment chamber disposed spaced from the transfer module around the transfer chamber to perform a first treatment process, and a second treatment chamber disposed around the transfer chamber to perform a second treatment process. | 2012-12-20 |
20120322016 | ROLLER HEARTH CALCINING FURNACE AND METHOD OF USE - A roller hearth calcining furnace that uses a movable fluid bed that is transported through plural heating modules, each heating module independently heated using an indirect heating system. The indirect heating system includes an oxidizer, an all-ceramic, indirect, air-to-air heat exchanger, and one or more metal heat exchangers. The oxidizer can be fired with oil, coal, natural gas or other means, is impervious to attack from dirty fuels, and produces clean hot air for productive use. The movable fluid bed includes a permeable silicon carbide ceramic plate that resides within, and is supported by, a sagger or tray. The sagger is transported on rollers through the plural heating modules, and thus different temperature zones within a furnace as required by the temperature profile of the material being calcined. | 2012-12-20 |
20120322017 | METHOD FOR HEATING A BLAST FURNACE STOVE - A method for heating a blast furnace stove includes combusting a fuel with a lower heating value (LHV) of 9 MJ/Nm | 2012-12-20 |
20120322018 | VIBRATING ORTHODONTIC REMODELLING DEVICE - A bite plate allowing for contact with occlusal and facial and/or lingual maxillary and mandibular teeth is coupled to an extraoral housing containing a rechargeable battery coupled to a vibrator coupled to a processor coupled to data and charging port or ports. The housing is at least water resistant, and a hatch allows access to the data and charging port or ports only. The device is fitted with a very smooth and quiet vibrator, with minimal variance on operating specifications. | 2012-12-20 |
20120322019 | Customized Orthodontic Arch Wire Manufactured Using Model of Patient's Teeth - Customized low force orthodontic arch wires include a core wire ( | 2012-12-20 |
20120322020 | Reduced-Friction Buccal Tube and Method of Use - An orthodontic appliance includes features for reducing friction between an interior of an archwire slot portion of the appliance and an archwire to be placed within the archwire slot. Other embodiments include a rounded exterior occlusal surface. Embodiments further include one or more receptacles for receiving an installation tool. | 2012-12-20 |
20120322021 | ORTHODONTIC ATTACHMENT BONDING TOOL AND METHOD OF USING SAME - The invention includes a dental tool for applying orthodontic attachments to teeth. The tool includes a body having a portion for holding the dental tool and a portion for selectively gripping an object to be bonded to a tooth. The tool also includes a nozzle for emitting pressurized air adjacent the portion for selectively gripping the object to be bonded to the tooth. A method of using the tool is also disclosed. | 2012-12-20 |
20120322022 | TOOTH VITALITY TESTER - One example embodiment includes a system for testing the vitality of a tooth. The system includes a stimulus output. The stimulus output is configured to produce a stimulus and output the stimulus to a tooth of a patient. The system also includes a controller, where the controller is configured to control the magnitude and duration of the stimulus produced by the stimulus output. | 2012-12-20 |
20120322023 | DEVICE FOR IDENTIFYING ORAL CONDITIONS - The present invention relates to devices and systems for detecting the existence of oral conditions. | 2012-12-20 |
20120322024 | STRIP FOR THE DELIVERY OF ORAL CARE COMPOSITIONS - Provided herein is a strip, shaped for the application to the labile surface of the front teeth and/or gums of a subject, which strip is provided with at least one oral care composition for delivery to a pre-determined area of the teeth and/or gums of the subject. Also provided herein are uses for delivery of compositions. Further provided are uses of the strip in combination with a dental device. | 2012-12-20 |
20120322025 | METHOD FOR FORMING DENTAL COATING AND DENTAL CAD/CAM DEVICE - A dental CAD/CAM device capable of accurately forming a dental coating is provided. The device includes: an intraoral-site measurement section | 2012-12-20 |
20120322026 | Dental Curing Lights and a Seamless, Single Load Cavity Preparation and Filling Technique - There are disclosed methods for the restoration of a decayed portion of a tooth, and dental matrices, dental wedges, interdental matrix stabilizers, dental separator rings, dental curing light devices, and kits that can be used in the methods for the restoration of a decayed portion of a tooth. | 2012-12-20 |
20120322027 | Apparatus and Methods for Determining the Location of the Apex of a Dental Root Canal - The invention relates to the field of medical methods and equipment and in preferred embodiments to equipment and methods used in dentistry. In particular it relates to an apparatus for determining the location of the apex of a dental root canal wherein the apparatus allows the acquisition of more accurate data in a shorter time span. | 2012-12-20 |
20120322028 | DENTAL SYRINGE - A dental syringe including a hollow piston having threads on the inner wall thereof, and an exterior threaded rod disposable within the hollow piston and upon rotation of the rod moving the piston longitudinally for dispensing dental material from the syringe. | 2012-12-20 |
20120322029 | DENTAL ISOLATOR - The dental isolator of the present invention is a one-time use dental device which is easy to use as well as easily customizable for the intended patient. The present invention can be used in the preparation and application of a pit and fissure sealant procedure. The dental isolator of the present invention can be adjusted and customized to tailor fit the mouth of the patient. The dental isolator of the present invention is positively retained within the patient's mouth and protects the isolated teeth from moisture on distal, lingual and facial exposure points. | 2012-12-20 |
20120322030 | MODULAR ABUTMENT SYSTEM FOR TILTED DENTAL IMPLANTS - A modular abutment system for offsetting an angle of tilted dental implants in a manner preserving structural stability of restoration components throughout the life of the restoration. A specialized deployment tool configurable to enable a practitioner to utilize available workspace during deployment. | 2012-12-20 |
20120322031 | METHOD AND APPARATUS FOR PREPARING DENTURE - A dental impression tray assembly includes an upper tray and a lower tray operable with the upper tray, and the lower tray includes a first piece configured to measure jaw relations of a patient's mouth and a pair of second pieces detachably attachable to the first piece. The first piece of the lower tray is inserted into the patient's mouth independently of the pair of second pieces of the lower tray to measure the jaw relations and to obtain a bite registration. After the first piece is connected to the pair of second pieces, the assembly allows a final gum impression of the patient's lower gum to be obtained. | 2012-12-20 |
20120322032 | METHODS AND SYSTEMS FOR ELECTRONIC MEAL PLANNING - Various methods and systems are provided for meal planning. In one example, a method for meal planning includes receiving a user's meal preferences and automatically generating a meal plan including a plurality of separate meals based on the user's meal preferences. | 2012-12-20 |
20120322033 | HEALTH AND FITNESS SYSTEMS - Systems and methods integrating childhood health and fitness into educational programs. Included are integrating multimedia entertainment concepts into health and fitness educational programs that are combined with multiple curricula. | 2012-12-20 |
20120322034 | SYSTEM AND METHOD FOR LANGUAGE INSTRUCTION USING VISUAL AND/OR AUDIO PROMPTS - A system and method for language instruction for implementation on a language instruction system that includes a computer system, is disclosed, wherein the method may include identifying a speech segment in a target language, that is susceptible to mispronunciation by language learners; selecting an auditory attribute for use in playing the identified speech segment by the language instruction system; altering a level of the auditory attribute to differ from a naturally occurring level of the attribute; and playing a first text sequence by the language instruction system, including at least one instance of the identified speech segment, using the altered level of the auditory attribute. | 2012-12-20 |
20120322035 | SYSTEM AND METHOD FOR ASSISTING LANGUAGE LEARNING - Speech data from the operation of a speech recognition application is recorded over the course of one or more language learning sessions. The operation of the speech recognition application during each language learning sessions corresponds to a user speaking, and the speech recognition application generating text data. The text data may a recognition of what the user spoke. The speech data may comprise the text data, and confidence values that are an indication of an accuracy of the recognition. The speech data from each language learning session may be analyzed to determine an overall performance level of the user. | 2012-12-20 |
20120322036 | COMPUTER-ASSISTED MEANS FOR ASSESSING LIFESTYLE RISK FACTORS - The present invention relates to methods of assessing disease susceptibility associated with dietary and lifestyle risk factors. The invention provides for analysis of alleles at loci of genes associated with lifestyle risk factors, and the disease susceptibility profile of an individual is determined by reference to datasets which further match the risk factor with lifestyle recommendations in order to produce a personalized lifestyle advice plan. | 2012-12-20 |
20120322037 | Anomaly Detection Educational Process - A process for educating a user to detect a visual target and a target-related anomaly in a visual field is provided that includes the collection of an image of a visual field displaying the target and the target-related anomalies. An image key is generated with location of the target and the target-related anomalies. The image is transformed into multiple panels that vary between one another as to a visual concealment parameter. A student user is then shown the multiple panels on a computer, with the user designating inputs into the computer as to user perceived location of the target and the target-related anomalies for each of the panels. Through scoring of the user inputs against the image key, the user is educated as to how to improve acuity and detection for target and anomaly detection. | 2012-12-20 |
20120322038 | SPORT TRAINING APPARATUS AND METHOD OF USE - A training apparatus having a harness mountable about a user's waist and thigh and a pull securable to the harness at a region corresponding to the iliac of a crest of the user for pulling the harness to encourage or resist turning of the user's hips to respectively teach or strengthen weight transfer from a back foot to a front foot of the user. A method is provided for training a person in an activity requiring transfer of weight and momentum from a lower area to an upper area of a person. | 2012-12-20 |
20120322039 | Portable Walking Trainer Device for Children - A portable walking trainer device for a child, having a U-shaped structure of support bars that are removably securable to a padded base mat. The padded base mat has apertures disposed along its perimeter to facilitate removable securement of the support bars to the mat. Securing posts attached to the support bars are inserted into the apertures, resulting in a stable set of handrails for a child to pull on, lean upon, or use for balance. All support bars are padded to reduce the risk of injury to a child user. When the device is not in use the support bars may be disassembled and fold up within the base mat. Handles disposed at the ends of the base mat allow the self-contained stowed device to be easily transported by a parent or caregiver. | 2012-12-20 |
20120322040 | SHAKEN BABY SYNDROME EDUCATIONAL DOLL - A doll for educating caregivers about Shaken Baby Syndrome comprises a head portion, a body, and may comprise transparent skin. The head portion may have a simulated brain disposed therein. The head portion is floppably connected to the body. The doll may further comprise a first chamber and a second chamber containing simulated blood, the simulated blood being visible in the first chamber after acceleration experienced during a shaking episode, but otherwise being concealed from view in the second chamber. A plurality of acceleration sensors may be disposed within the doll to detect acceleration experienced during a shaking episode, and the plurality of acceleration sensors may trigger warnings to the caregiver when portions of the doll experience acceleration sufficient to endanger an infant. | 2012-12-20 |
20120322041 | METHOD AND APPARATUS FOR PRODUCING AND DELIVERING CUSTOMIZED EDUCATION AND ENTERTAINMENT - A parent provides information regarding a child and other family members to a system. The information is then used to create customized assemblages of linear and interactive media, which may be further personalized for that child's entertainment and education. The customized and personalized episode so assembled persists and can be replayed indefinitely. New episodes are created regularly (e.g., daily), and can be based on the child's age, preferences, and performance while experiencing previous episodes. Scheduled events (e.g., birthdays, starting school), local current events (e.g., weather), the current situation (e.g., current location, time of day), etc. may also influence the creation of new episodes. While monitoring the child's progress, the system provides to a parent suggestions for activities related to the child's recent episodes. | 2012-12-20 |
20120322042 | PRODUCT SPECIFIC LEARNING INTERFACE PRESENTING INTEGRATED MULTIMEDIA CONTENT ON PRODUCT USAGE AND SERVICE - A computer implemented method that includes providing a product specific learning interface comprising integrated multimedia content on product usage and service to a user. The multimedia content demonstrates a feature of a product stored in a database ( | 2012-12-20 |
20120322043 | ADAPTIVELY-SPACED REPETITION LEARNING SYSTEM AND METHOD - An adaptively-spaced repetition learning system and method facilitate the learning of material over time through the presentation of the material at ever-increasing intervals. Embodiments of the system and method present a stimulus-response pair to a student to obtain a binary response that is either correct or incorrect. The token is next presented to the student based on the student's response. If the response is correct, then the token is presented to the student based on the initial repetition interval without modification. If the response is incorrect, then the learnedness value is reset and the progression through the repetition intervals is restarted. In some embodiments, the initial repetition intervals are dynamically adjusted based on the student's response to obtain revised repetition intervals. This is achieved by manipulating the exponent of the mathematical term representing the curve of the repetition intervals. | 2012-12-20 |
20120322044 | SYSTEMS, METHODS, AND MEDIA FOR PROVIDING VIRTUAL MOCK TRIALS - Systems, methods, and media for providing virtual mock trials are provided. A system includes one or more hardware processors that are operative to initiate an interactive mock trial session via a network to a plurality of remote participants. The one or more hardware processors generate first and second presentation displays of the mock trial, each having an arrangement of content that may include a live-action video, a pre-recorded video, an image, interactive actuators or icons, text, or combinations thereof. The first display has a first set of interactive options and is transmitted to a plurality of mock jurors, while the second display has a second set of interactive options and is transmitted to at least one other remote participant, which may be, for example, a trial manager, an attorney, or an observer. Evaluations of the mock trial are interactively completed and received by the hardware processor. | 2012-12-20 |
20120322045 | METHOD FOR ISOLATING CELLULAR PRODUCTS BY CRYOPRESERVATION - Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a tissue having desired cells that are less prone to destructive freezing and undesired cells that are more prone to destructive freezing, or pre-treating a tissue to have such characteristics. The methods may involve freezing the tissue, disrupting the tissue, warming the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may thereby provide an enzyme-free or reduced-enzyme method of isolating a cellular product that is more consistent, reliable and less toxic than conventional methods. The methods may also yield an optimum quantity of cellular product that retain sufficient functional integrity to be useful as a transplantation resource. | 2012-12-20 |
20120322046 | METHOD FOR DETECTING CELL AND CELL DETECTING SYSTEM - The detection of rare cells can be achieved with high efficiency, without deteriorating the sensitivity of the detection. A detection method which enables the above-mentioned detection comprises: a first step of carrying out a pretreatment for removing erythrocytes from blood to prepare a cell suspension containing leukocytes and rare cells; a second step of providing all of the cells contained in the cell suspension produced in the first step onto an observation area having multiple holes formed thereon; a third step of taking an optical image of the cells that have been provided on the observation area; and a forth step of detecting the rare cells from the image obtained by the image taking in the third step. | 2012-12-20 |
20120322047 | Apparatus And Method For Cell Exploitation - The present invention relates to an apparatus ( | 2012-12-20 |
20120322048 | Materials and Methods for Assessing and Mapping Microbes and Microbial Biofilms on Wounds - The subject invention provides point-of-care assays for assessing the topographical distribution of microbial biofilm and/or specific microorganisms in wounds. | 2012-12-20 |
20120322049 | MATERIALS AND METHODS FOR DETECTION OF HPV NUCLEIC ACIDS - Provided are nucleic acids capable of hybridizing to HPV 16 and/or HPV 18 nucleic acids, in particular, mRNA encoding E2 and E6-7 gene products. Such nucleic acids are useful in methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, methods and means for predicting the progression of precancerous cervical lesions, and methods and means for detecting disruption of genes or gene expression. | 2012-12-20 |
20120322050 | USING IMPEDANCE-BASED CELL RESPONSE PROFILING TO IDENTIFY PUTATIVE INHIBITORS FOR ONCOGENE ADDICTED TARGETS OR PATHWAYS - Use of impedance devices in methods of generating a time dependent cellular profiles (TCRP) for the modulation of oncogene addicted cells and comparing the impedance-based TCRP to controls or knowns to identify signature time dependent cellular profiles. | 2012-12-20 |
20120322051 | METHODS AND COMPOSITIONS FOR IDENTIFYING AND CHARACTERIZING HEPATITIS C - The invention provides novel methods and compositions for amplifying portions of the HCV genome. The nucleic acid sequences set forth as SEQ ID NOS:1-64 derived from HCV cDNA and functional equivalents thereof, kits containing same, and methods employing same, are useful for the identification and characterization of HCV in biological samples. | 2012-12-20 |
20120322052 | SYSTEM AND METHOD FOR PREPARING SAMPLES - A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest. | 2012-12-20 |
20120322053 | CROSS-COUPLED PEPTIDE NUCLEIC ACIDS FOR DETECTION OF NUCLEIC ACIDS OF PATHOGENS - The present invention concerns methods for detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA having a first cross-reactive functional group with a substrate having a second PNA affixed thereto, the second PNA having a second first cross-reactive functional group, wherein the first PNA has a reporter molecule attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. | 2012-12-20 |
20120322054 | Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 2012-12-20 |
20120322055 | Molecular Dispensers - A method for dispensing charged particles includes applying a bias voltage to promote motion of charged molecules through a nanopore, detecting passage of at least one charged molecule through the nanopore, and manipulating an electrostatic potential barrier inside the nanopore, so as to prevent movement of additional charged molecules through the nanopore. | 2012-12-20 |
20120322056 | RNA-EXIT-CHANNEL: TARGET AND METHOD FOR INHIBITION OF BACTERIAL RNA POLYMERASE - The invention provides a target and methods for specific binding and inhibition of RNAP from bacterial species. The invention is directed to a method for identifying agents that bind to a bacterial RNAP homologous RNA-exit-channel amino-acid sequence, comprising preparing a reaction solution comprising the agent to be tested and an entity comprising a bacterial RNAP homologous RNA-exit-channel amino-acid sequence, and detecting presence or amount of binding. The invention has applications in control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy. | 2012-12-20 |
20120322057 | POLYMERASE COMPOSITIONS AND METHODS - Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein. | 2012-12-20 |
20120322058 | Analysis of nucleic acids - Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles. | 2012-12-20 |
20120322059 | MUTATION WITHIN THE CONNEXIN 26 GENE RESPONSIBLE FOR PRELINGUAL NON-SYNDROMIC DEAFNESS AND METHOD OF DETECTION - A purified polynucleotide having a chain of nucleotides corresponding to a mutated sequence, which in a wild form encodes a polypeptide implicated in hereditary sensory defect, wherein said mutated purified polynucleotide presents a mutation responsible for prelingual non-syndromic deafness selected from the group consisting of a specific deletion of at least one nucleotide. | 2012-12-20 |
20120322060 | DIAGNOSIS AND MONITORING TREATMENT OF PSYCHIATRIC DISEASES WITH SPADIN AND RELATED METHODS - A method of diagnosing depression in a subject, comprising analyzing a biological sample from a subject in need of diagnosis of depression for the expression of spadin, detecting and measuring the amount of spadin in the sample, and diagnosing depression in the subject if an increase or a decrease in spadin expression in the biological sample is detected compared to a control, is described as are methods for monitoring treatment, remission and the course of the disease. Related kits are also described. Also described is a method for identifying candidate compounds for treating depression. | 2012-12-20 |
20120322061 | MACROMOLECULAR COMPOUND FOR DETECTING TARGET MATERIAL AND METHOD OF DETECTING TARGET MATERIAL BY USING THE SAME - Macromolecular compound for detecting a target material in a biological sample, and a method of using same. | 2012-12-20 |
20120322062 | Methods And Compositions For Detection Of Cowden Syndrome (CS) and CS-Like Syndrome - The invention is directed to methods of detecting Cowden syndrome (CS) or CS-like syndrome in an individual or determining whether an individual is at risk for developing Cowden syndrome (CS) or CS-like syndrome comprising detecting the presence of a mutated succinate dehydrogenase B (SDHB), mutated succinate dehydrogenase D (SDHD) or combination thereof in the individual, wherein detection of a mutated SDHB, SDHD or a combination thereof indicates that the individual is positive for, or at risk of developing, CS or CS-like syndrome. The invention is also directed to an article of manufacture for detecting Cowden syndrome (CS) or Cowden-like syndrome in an individual, comprising one or more agents that detects mutated succinate dehydrogenase B (SDHB), mutated succinate dehydrogenase D (SDHD) or combination thereof in the individual, and instructions for use. | 2012-12-20 |
20120322063 | METHODS FOR QUANTIFYING MICRORNA PRECURSORS - The present invention is directed to methods, reagents, kits and compositions for identifying and quantifying microRNA (miRNA) precursor expression in a biological sample. The method uses gene-specific primers and reverse transcriptase to convert the primary miRNA precursors (pri-miRNA) and pre-miRNA precursors (pre-miRNAs) to cDNA. The method also uses amplification reactions using gene specific forward and reverse primers that are targeted to the hairpin sequence of pri- and pre-microRNA precursors to detect the expression levels of both the pri- and the pre-micoRNAs. In one embodiment, the amplification reaction is a real-time PCR wherein the level of PCR amplification products produced is related to the levels of the microRNA precursors in the biological sample. In another embodiment, a probe is used to distinguish between similar isoforms of microRNA precursors. In another embodiment, the expression levels of a pre-miRNA precursor is calculated by using primers and amplification reactions that detect the pri-miRNA together with amplification reactions and primers that detect both pri- and pre-miRNAs, and calculating the difference. | 2012-12-20 |
20120322064 | HYBRIDIZATION PROBES AND METHODS OF THEIR USE - Hybridization probes for hybridizing to the same target nucleic acid are disclosed, the hybridization probes comprising an electrically-active magnetic nanoparticle-labeled detector probe and a capture probe including a conjugating moiety for immobilization. Also disclosed is a biodetection method including the steps of: providing hybridization probes for hybridizing to the same target nucleic acid, the hybridization probes comprising an electrically-active magnetic nanoparticle-labeled detector probe and a capture probe; hybridizing the target nucleic acid with each of the electrically-active magnetic nanoparticle-labeled detector probe and a capture probe in a sample including the target nucleic acid; magnetically separating the hybridized target nucleic acid from the sample; capturing the hybridized target nucleic acid on a substrate through the capture probe; and measuring the oxidation-reduction signal of the electrically-active magnetic nanoparticle-labeled detector probe. | 2012-12-20 |
20120322065 | Methods for Use with Nanoreactors - The invention relates to methods of using nanoreactor technology for sample analysis in microfluidic systems. | 2012-12-20 |
20120322066 | Development of Novel Detergents for Use in PCR Systems - This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described. | 2012-12-20 |
20120322067 | ASSAY METHOD FOR TARGET NUCLEIC ACID BY SIGNAL AMPLIFICATION USING PROBE HYBRIDIZATION AND RESTRICTION - Oligonucleotides for detection of nucleic acid in a sample that provide for an amplified signal by recycling probes and probe fragments. | 2012-12-20 |
20120322068 | METHOD FOR IDENTIFYING INCREASED RISK OF ANXIETY DISORDERS - The present invention is for methods for identifying a human having an increased risk for anxiety disorders. The method involves genotyping the human for a specific brain-derived neurotrophic factor (BDNF) single nucleotide polymorphism (SNP), and/or administering a fear conditioning procedure while measuring fear, and administering an extinction procedure while measuring fear. The method can also involve comparing/MRI images of the amygdala of the human acquired during conditioning and extinction and determining if the human is unresponsive to extinction therapy by noting heightened and non-declining activity in the amygdala during extinction. | 2012-12-20 |
20120322069 | Diagnositic Methods of Tumor Susceptibility With Nucleotide Polymorphisms Inside MicroRNA Target Sites - Methods of diagnosing tumor susceptibility or cancer including the step of determining whether a patient has one or more SNP-miRNA expression pattern combinations described herein. Each SNP-miRNA expression pattern combination is supported by data that shows that the SNP is associated with tumor susceptibility because of its ability to affect miRNA binding sites and/or miRNA:mRNA gene regulation. | 2012-12-20 |
20120322070 | CRYOGENIC BIOPSY SYSTEM AND METHOD - A cryogenic biopsy device is configured to provide thin, frozen tissue samples, which may be viewed under a microscope and selected for biomarker analysis. The device is configured to provide slices which are less than 50 micrometers in thickness, while snap-freezing the tissue and maintaining the sample in a deep-frozen state until it reaches the pathology lab for further processing. Alternatively, thicker slices can be harvested by the device and additional sectioning can be done in the pathology lab by using cryomicrotome. The device of the present invention may be used in rigid instruments and in flexible devices, such as endoscopes, for example, and may be suitable for single use or multiple use. | 2012-12-20 |
20120322071 | METHOD FOR QUANTITATIVE MEASUREMENT OF GENE EXPRESSION FOR INDENTIFYING INDIVIDUALS AT RISK FOR BRONCHOGENIC CARCINOMA - A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed. | 2012-12-20 |
20120322072 | QUANTIFICATION OF A MINORITY NUCLEIC ACID SPECIES - The technology relates in part to quantification of a minority nucleic acid species from a nucleic acid sample. In some embodiments, methods for determining the amount of fetal nucleic acid (e.g. absolute amount, relative amount) in a maternal sample are provided. | 2012-12-20 |
20120322073 | METHODS FOR THE TREATMENT OF CANCER AND INFLAMMATORY DISEASES USING CEREBLON AS A PREDICTOR - Uses of the protein cereblon as a predictor of clinical sensitivity to cancer, inflammatory diseases, and patient response to drug treatment. | 2012-12-20 |
20120322074 | Prostate Tumor Markers and Methods of Use Thereof - Newly identified proteins as markers for the detection of prostate tumors, or as targets for their therapeutic treatment, affinity ligands capable of selectively interacting with said markers as well as methods for tumor diagnosis and therapy using the same. | 2012-12-20 |
20120322075 | Lung Tumor Markers and Methods of Use Thereof - Newly identified proteins as markers for the detection of lung tumors, or as therapeutic targets for their treatment, affinity ligands capable of selectively interacting with the newly identified markers and methods for tumor diagnosis and therapy using such ligands. | 2012-12-20 |
20120322076 | MICROCHAMBER ELECTROCHEMICAL CELL HAVING A NANOSLOT - A microchamber electrochemical cell and method of using the cell for performing quantitative analysis of various charged macromolecules is presented. The microchamber electrochemical cell includes a substrate, opposing electrodes and at least one nanoslot. The substrate is configured to define a pair of opposing fluid reservoirs. The pair of opposing electrodes are respectively positioned within the opposing fluid reservoirs. Each nanoslot is configured to fluidly connect the opposing fluid reservoirs together. The opposing fluid reservoirs of the microchamber electrochemical cell are fluidly connected to each other only through each nanoslot. Each nanoslot is physically restricted to less than 500 nanometers. One method includes the steps of coupling, filling, measuring, obtaining, performing and preparing. | 2012-12-20 |
20120322077 | CELL, METHOD, AND ASSAY KIT FOR MEASURING LEVEL OF ARYL HYDROCARBON RECEPTOR TRANSCRIPTIONAL ACTIVATION - According to one embodiment, a cell for measuring a level of Ah receptor transcriptional activation is provided. The cell is derived from a neural cell. The cell contains a chromosome into which a reporter construct and an Ah receptor gene are introduced. The reporter construct has a sequence represented by SEQ ID NO: 1 and a reporter gene operably linked to the downstream of the nucleotide sequence. The sequence represented by SEQ ID NO: 1 has a recognizing sequence of an Ah receptor and a nucleotide sequence which is operably linked to the downstream of the recognizing sequence and required to initiate transcription. | 2012-12-20 |
20120322078 | Production of Propanols, Alcohols, and Polyols in Consolidated Bioprocessing Organisms - The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, up-regulated or down-regulated. | 2012-12-20 |
20120322079 | METHOD FOR TESTING OR SCREENING PROTEIN SYNTHESIS INHIBITORS - This invention provides methods for screening an inhibitor of protein synthesis by measuring the level of relocalization of an SMN complex component from the cytoplasm to the nucleus. Additionally, the invention provides a kit and a system for screening protein synthesis inhibitors in a cell. | 2012-12-20 |
20120322080 | Measurement of PKA for Cancer Detection - The present invention relates to a method of detecting the presence of cancer by measuring the level of enzyme activity and autoantibodies in the blood of an individual. In particular the present invention relates to methods for measurement of activated cAMP-dependent protein kinase A (PKA) activity and antibodies to PKA, a kit for activated PKA activity measurement, and the use of the measured levels of these analytes for determining the presence of cancer. | 2012-12-20 |
20120322081 | METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN - Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as | 2012-12-20 |
20120322082 | BIOMARKER FOR AMYOTROPHIC LATERAL SCLEROSIS AND DIAGNOSTIC KIT AND METHOD USED THEREOF - A diagnostic marker for determining of a patient with amyotrophic lateral sclerosis (ALS), comprising an autoantibody against a human protein HMGB1 is described. A diagnostic kit for determining of a patient with ALS, comprising: (a) an HMGB1 protein; and (b) an HMGB1 antibody standard is also presented. A method of determining ALS in a subject, comprising the steps: (a) detecting autoantibody against HMGB1 in a biological sample taken from the subject and normal control individuals; and (b) statistically comparing concentrations of the autoantibody against HMGB1 in the subject with that of the normal control individuals obtained from step (a); wherein a higher concentration of the autoantibody against HMGB1 in the subject than a cut-off concentration indicates the subject suffers from ALS is further described. | 2012-12-20 |
20120322083 | ANTIBODY BINDING TO ABCA1 POLYPEPTIDE - The invention relates to an isolated antibody that binds to native ABCA1 polypeptide and its uses for the detection of ABCA1 polypeptides in tissue samples. | 2012-12-20 |
20120322084 | CELL LINES COMPRISING SOUR-TASTE RECEPTORS - The present invention relates to sour taste receptors and compositions and methods thereof. In particular, the present invention provides assays and methods of screening for ligands specific for sour taste receptors. Additionally, the present invention provides methods for screening for accessory proteins and mutations, polymorphisms and other potential sour taste receptor protein mutations that are associated with disease states, and therapeutic agents, ligands, and modulators of such proteins. The present invention also provides compositions and methods for modulating sour taste receptors in vitro and in vivo. | 2012-12-20 |
20120322085 | THERAPEUTIC AGENT FOR AUTOIMMUNE DISEASES OR ALLERGY, AND METHOD FOR SCREENING FOR THE THERAPEUTIC AGENT - Disclosed is a therapeutic agent for treating a cellular immune disease, comprising as an active ingredient a substance that inhibits binding between Sema3A and a Neuropilin-1/Plexin-A1 heteroreceptor. The substance includes, for example, a Sema3A neutralizing antibody, a Neuropilin-1 neutralizing antibody, or a soluble Neuropilin-1 or derivative thereof. Also disclosed is a method for screening a therapeutic agent for treating a cellular immune disease utilizing a signal generated by the interactions of Neuropilin-1, Plexin-A1 and Sema3A as a marker. | 2012-12-20 |
20120322086 | TESTING DEVICE FOR IDENTIFYING ANTIGENS AND ANTIBODIES IN BIOFLUIDS - A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone. | 2012-12-20 |
20120322087 | METHOD FOR EXAMINING ACUTE RENAL DISORDER - Provided is a test method for acute kidney injury, including detecting urinary podocalyxin. According to the test method, a subject to be tested who has a higher value for the urinary podocalyxin than a reference value can be assessed to have acute kidney injury. Further, as compared to a conventional method, the test method allows acute kidney injury to be assessed accurately and non-invasively, which allows a physical burden on a patient to be reduced. Thus, the test method is useful. | 2012-12-20 |
20120322088 | METHODS FOR THE DIAGNOSIS, RISK ASSESSMENT, AND MONITORING OF AUTISM SPECTRUM DISORDERS - Methods for the diagnosis, risk assessment, and monitoring of Autism Spectrum Disorder (ASD) are disclosed. More specifically the present invention relates to the measurement of small molecules (metabolites) in human plasma that are found to have different abundances between persons with a clinical manifestation of ASD and subjects not expressing symptoms of ASD. Further, this invention relates to the monitoring of putative therapeutic strategies designed to ameliorate the biochemical abnormalities associated with ASD. | 2012-12-20 |
20120322089 | SENSITIVE AND GENERIC ANTIBODIES AND MULTIPLE APPLICATIONS - The invention relates to the detection and quantification of carbamazepine drugs and their metabolites. The invention is underpinned by novel polyclonal antibodies with unique binding properties which enable immunoassay methods and kits for various applications. | 2012-12-20 |
20120322090 | SOLID PHASE-BOUND ELASTASE-BINDING ASSAY FOR THE MEASUREMENT OF ALPHA1-ANTITRYPSIN ACTIVITY - The present invention relates to a method for the measurement of active alpha | 2012-12-20 |
20120322091 | PHARMACOLOGIC METHOD OF LOWERING CHOLESTEROL PRODUCTION - The present invention provides a screening method for identifying test agents that modulate cell membrane cholesterol activity. The modulating activity of the test agents may be measured using lytic compounds, which cause cell lysis or increases in cell permeability in response to cell membrane cholesterol levels. The invention further provides therapeutic agents that are identified using the screening method. The therapeutic agents either increase or decrease the cell membrane cholesterol activity, cholesterol concentration and/or both in vivo and/or in vitro. | 2012-12-20 |
20120322092 | Resonance Energy Transfer Assay with Synaptobrevin Substrate Moiety - Compositions and methods for analyzing intracellular BoNT protease activity, and especially BoNT/B, BoNT/G, BoNT/D, and/or BoNT/F protease activity are provided. Most preferably, cells express one or more recombinant hybrid proteins that include one or more fluorescent proteins and at least one BoNT protease recognition and cleavage sequence, and analysis is performed using FRET analysis. | 2012-12-20 |
20120322093 | METHOD FOR THE DETERMINATION OF SEQUENCE VARIANTS OF POLYPEPTIDES - The current invention is directed to a method for determining amino acid sequence mutations in a produced polypeptide, comprising the following steps of a) providing a sample of a produced polypeptide, b) incubating the polypeptide in the sample with a protease, c) performing a two dimensional analysis using reversed phase chromatography coupled with a high resolution mass spectroscopy (FT-ICR/FT-orbitrap) and MS/MS analysis of the amino acid sequence fragments of the peptides, d) data evaluation by comparing the LC-MS data sets obtained for the samples side by side with the data set of a reference sample, by searching for differences in the signal intensities at given retention times and by evaluation of differential signals with respect to amino acid sequence mutations. The reference sample for data evaluation (d) can be either a well characterized standard or one of the samples to be analyzed. | 2012-12-20 |
20120322094 | HIGH-MOLECULAR-WEIGHT ADIPONECTIN MEASUREMENT METHOD - Provided is a method of separating and measuring highly active HMW adiponectin in adiponectin multimers. A method of measuring high-molecular-weight adiponectin in a sample, wherein adiponectin multimers are separated by use of a protease and measured immunologically, the method comprising reacting a sample containing adiponectin multimers with chymotrypsin. | 2012-12-20 |
20120322095 | Novel 2,5-Diketo-L-Gluconic Acid Reductases and Methods Of Use - Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described. | 2012-12-20 |