52nd week of 2019 patent applcation highlights part 26 |
Patent application number | Title | Published |
20190390163 | GENERATION OF HEPATOCYTES FROM PLURIPOTENT STEM CELLS - Methods are provided for producing differentiated cells from stem cells, including producing hepatocytes. Compositions thereof are also provided, as are methods of treating a liver disorder. | 2019-12-26 |
20190390164 | METHODS FOR STAINING CELLS FOR IDENTIFICATION AND SORTING - The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided. | 2019-12-26 |
20190390165 | COMPOSITION FOR CULTURING BRAIN ORGANOID BASED ON DECELLULARIZED BRAIN MATRIX AND METHOD FOR PREPARING SAME - Provided is a method for preparing a composition for culturing a brain organoid, the method comprising (a) decellularizing brain tissue; (b) drying the brain tissue; and (c) gelating the brain tissue. | 2019-12-26 |
20190390166 | PLATELET-RICH PLASMA COMPOSITIONS AND METHODS OF PREPARATION - Methods are described for using compositions containing platelet-rich plasma for the treatment of a variety of tissue lesions, and the use of compositions containing platelet-rich plasma to accelerate or complete the differentiation of a cell line. Particularly, use of platelet-rich plasma in conjunction with cell differentiation. | 2019-12-26 |
20190390167 | METHOD FOR PRODUCING CARTILAGE CELLS INDUCED TO BE DIFFERENTIATED FROM STEM CELLS - The present invention relates to a method for inducing differentiation, into chondrocytes, of cord blood mononuclear cell-derived induced pluripotent stem cells. In a case where a chondrogenic pellet produced by the method of the present invention is transplanted into a cartilage damage area in vivo, regeneration of cartilage can be effectively exhibited by differentiated chondrocytes. In such a case, an effective cartilage regeneration capacity can be exhibited as compared with a case where chondrocytes produced by differentiation induction with the addition of a recombinant growth factor are transplanted. Thus, the present invention can be usefully used for tissue engineering therapies. | 2019-12-26 |
20190390168 | PLURIPOTENT STEM CELL ASSAY - The invention relates to a method for detecting residual, undifferentiated pluripotent stem cells (PSCs) in a culture of cells differentiated from PSCs, the method comprising:
| 2019-12-26 |
20190390169 | PANCREATIC PROGENITOR CELL PRODUCTION METHOD - The present invention relates to a method for producing pancreatic progenitor cells from pluripotent stem cells. More specifically, the present invention relates to a method for producing pancreatic progenitor cells, comprising causing the action of a factor having the inhibitory activity for cyclin-dependent kinase 8 and/or cyclin-dependent kinase 19 (hereinafter, also abbreviated to CDK8/19). | 2019-12-26 |
20190390170 | METHODS FOR MODULATING PRODUCTION PROFILES OF RECOMBINANT PROTEINS - The present invention relates to methods and compositions for culturing a host cell expressing a recombinant protein in a cell culture medium supplemented with feeds comprising effective amounts of valeric acid, whereby viability of the cells and production of said protein are increased relative to cells grown with feeds comprising no valeric acid. | 2019-12-26 |
20190390171 | CELL CULTURE MEDIUM FOR CULTURING ORGANOID, CULTURE METHOD, AND ORGANOID - A cell culture medium for culturing organoid containing at least two types of components selected from the group consisting of insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epiregulin (EREG), and at least one type of component among the following components i) to iii): i) Wnt agonist, ii) bone morphogenetic protein (BMP) inhibitor, and iii) transforming growth factor-β (TGF-β) inhibitor. | 2019-12-26 |
20190390172 | AGENT FOR REMOVING UNDIFFERENTIATED iPS CELLS - The present invention provides a compound of formula (1) or a salt thereof wherein ring Q | 2019-12-26 |
20190390173 | AGENT FOR ACCELERATING GROWTH OF PLURIPOTENT STEM CELLS - An object of the present invention is to provide a material capable of further accelerating growth of pluripotent stem cells, such as pluripotent stem cells, without impairing pluripotency thereof. In other words, the invention is an agent for accelerating growth of pluripotent stem cells, containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof as an active ingredient; and is a method for culturing pluripotent stem cells, including culturing pluripotent stem cells in a culture medium that contains a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof. | 2019-12-26 |
20190390174 | METHODS AND PRODUCTS FOR TRANSFECTING CELLS - The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed. | 2019-12-26 |
20190390175 | BACTERIAL DELIVERY VEHICLE, PROCESS OF PRODUCTION AND USES THEREOF - The present invention relates to the delivery of a payload by bacterial delivery vehicle, i.e. the encapsulation and the delivery of a single plasmid by different bacterial virus particles. More specifically, the present invention concerns a pharmaceutical composition comprising a payload packaged in at least two different bacterial delivery vehicles and a method of production thereof. | 2019-12-26 |
20190390176 | NOVEL RECOMBINANT ADENO-ASSOCIATED VIRUS CAPSIDS WITH ENHANCED HUMAN SKELETAL MUSCLE TROPISM - The present invention relates to variant AAV capsid polypeptides, wherein the variant AAV capsid polypeptides exhibit increased transduction and/or tropism in human muscle tissue or cells as compared non-variant parent capsid polypeptides. | 2019-12-26 |
20190390177 | RECOMBINANT POLYMERASES WITH INCREASED PHOTOTOLERANCE - Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template. | 2019-12-26 |
20190390178 | Variants of Terminal Deoxynucleotidyl Transferase and Uses Thereof - The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2. 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment. | 2019-12-26 |
20190390179 | SYNTHETIC REVERSE TRANSCRIPTASES AND USES THEREOF - The present disclosure provides non-natural reverse transcriptases for conducting reverse transcription. The non-natural reverse transcriptases herein may have increased thermostability and can conduct reverse transcription more efficiently than natural reverse transcriptases. | 2019-12-26 |
20190390180 | GENE CONSTRUCT ENCODING MUTANT THIOESTERASE, MUTANT THIOESTERASE ENCODED THEREBY, TRANSFORMED HOST CELL CONTAINING THE GENE CONSTRUCT, AND METHOD OF USING THEM TO PRODUCE MEDIUM-CHAIN FATTY ACIDS - Unnatural, mutated thioesterases having an amino acid sequence that is at least 80% identical to SEQ ID NO:1 and having substitutions at one or more of amino acid positions I107, R108, L109, S122, M141, E142, Y145, and L146, gene constructs encoding and configured to express the mutated thioesterases in a transformed host cell, and host cells transformed to contain the gene constructs. | 2019-12-26 |
20190390181 | Polynucleotides Encoding Lipoprotein Lipase for the Treatment of Hyperlipidemia - The invention relates to mRNA therapy for the treatment of hyperlipidemia. mRNAs for use in the invention, when administered in vivo, encode human lipoprotein lipase (LPL), isoforms thereof, functional fragments thereof, and fusion proteins comprising LPL. mRNAs of the invention are preferably encapsulated in lipid nanoparticles (LNPs) to effect efficient delivery to cells and/or tissues in subjects, when administered thereto, mRNA therapies of the invention increase and/or restore deficient levels of LPL expression and/or activity in subjects. mRNA therapies of the invention further decrease levels of triglycerides associated with deficient LPL activity in subjects. | 2019-12-26 |
20190390182 | LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2019-12-26 |
20190390183 | ENZYME COMPOSITION AND USES THEREOF - The invention relates to enzyme compositions comprising a glucoamylase, an alpha-amylase, and optionally a cellulolytic composition and/or a protease. The invention also relates to the use thereof in processes of producing sugars and/or fermentation products from starch-containing material by saccharifying and/or fermenting starch-containing material at a temperature below the initial gelatinization temperature. | 2019-12-26 |
20190390184 | ACID-ALPHA GLUCOSIDASE VARIANTS AND USES THEREOF - The present invention relates to variants of acid-alpha glucosidase and uses thereof. Said variants are sequence-optimized and/or are linked to a heterogenous signal peptide. | 2019-12-26 |
20190390185 | POLYPEPTIDES HAVING PROTEASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention further relates to the use of such polypeptides in detergent and/or in cleaning processes. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. | 2019-12-26 |
20190390186 | COMPOSITIONS AND METHODS FOR IDENTIFYING RNA BINDING POLYPEPTIDE TARGETS - The present invention features compositions comprising fusion polypeptides comprising an RNA binding polypeptide operationally linked to an RNA modifying enzyme (e.g., adenosine deaminase, cytidine deaminase), and methods of use therefore. | 2019-12-26 |
20190390187 | ENGINEERED PRIMATE L-METHIONINASE FOR THERAPEUTIC PURPOSES - Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids. | 2019-12-26 |
20190390188 | HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME - Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. | 2019-12-26 |
20190390189 | DONOR REPAIR TEMPLATES MULTIPLEX GENOME EDITING - The present disclosure provides improved multiplex genome editing compositions and methods. The disclosure further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of a hemoglobinopathy, a cancer, an infectious disease, an autoimmune disease, an inflammatory disease, or an immunodeficiency. | 2019-12-26 |
20190390190 | SYSTEMS AND METHODS FOR MASSIVELY PARALLEL COMBINATORIAL ANALYSIS OF SINGLE CELLS - Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery. | 2019-12-26 |
20190390191 | CONSTRUCTING ENZYME-BASED SENSORS - Provided among other things is a method to make a sensor enzyme that comprises: (1) identifying an area on the surface of an enzyme having one to three targeted loops including one linked loop, the loops having 3 or more residues without backbone hydrogen bonding and having up to 10 residues designated as the targeted loop, (2) creating an expression library of more than a thousand recombinant clones expressing recombinant enzyme wherein a segment within one, two or all of the targeted loops are replaced with from three to fourteen amino acid residue segments, (3) performing selection or screening of such library for (a) binding to molecule target substance; (b) enzymatic activity; and (c) allosteric modulation of enzymatic activity by the target substance, and (4) identifying one or more recombinant clones expressing recombinant enzyme that is allosterically modulated by the target substance. | 2019-12-26 |
20190390192 | METHODS AND COMPOSITIONS FOR RAPID NUCLEIC ACID LIBRARY PREPARATION - Rapid nucleic acid libraries, methods of generation, kits, and compositions relating to library synthesis, including reagents, intermediaries and final products are disclosed herein. The disclosure enables rapid synthesis of libraries that allow independent verification of sequence information and rapid identification of sequence information with template of origin. | 2019-12-26 |
20190390193 | Reversible method for sustainable human cognitive enhancement - A reversible method for general-purpose cognitive enhancement in mentally healthy adults comprising administering an RNA-editing ribonuclease complexed with a single guide RNA to lower the population of 5-hydroxytryptamine 2A receptors in the brain. | 2019-12-26 |
20190390194 | HIGH-DENSITY DNA STORAGE WITH SALT - A data storage medium is disclosed comprising a dried product formed by drying a salt solution dried together with artificially synthesized DNA molecules encoding digital information. The cation in the salt may be calcium, magnesium, lanthanum, or another cation. The anion in the salt may be chloride, phosphate, or another anion. The DNA is protected from degradation by drying with the salt. Stored DNA may be freed from the salt for sequencing or other analysis by mixing the dried product with a chelator. The dry product formed from DNA and a salt may contain more than 30% DNA by weight and degrade at rates that are less than a third of rate at which untreated DNA degrades. | 2019-12-26 |
20190390195 | MODIFIED GUIDE RNAS, METHODS AND USES - The present invention discloses modified guide RNAs which can be used with the CRISPR CAS 9 system for modifying gene expression, particularly in mammalian cells for medical or research uses. | 2019-12-26 |
20190390196 | 3'END CAPS FOR RNAi AGENTS FOR USE IN RNA INTERFERENCE - The disclosure relates to novel compounds and compositions comprising a RNAi agent comprising a novel compound as a 3′ end cap. The disclosure also relates to processes for making such compositions, and methods and uses of such compositions, e.g., to mediate RNA interference. | 2019-12-26 |
20190390197 | OLIGONUCLEOTIDE COMPOSITIONS AND METHODS THEREOF - Among other things, the present disclosure relates to designed oligonucleotides, compositions, and methods thereof. In some embodiments, provided oligonucleotide compositions provide altered splicing of a transcript. In some embodiments, provided oligonucleotide compositions have low toxicity. In some embodiments, provided oligonucleotide compositions provide improved protein binding profiles. In some embodiments, provided oligonucleotide compositions have improved delivery. In some embodiments, provided oligonucleotide compositions have improved uptake. In some embodiments, the present disclosure provides methods for treatment of diseases using provided oligonucleotide compositions. | 2019-12-26 |
20190390198 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF APLHA-1 ANTITRYPSIN BY DOUBLE-STRANDED RNA - This invention relates to compounds, compositions, and methods useful for reducing α-1 antitrypsin target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents. | 2019-12-26 |
20190390199 | Aptamers for Use in Inhibition and/or Suppression of TLR9 Activation - The present invention relates to new aptamer molecules for use in therapy of a subject by inhibiting or suppressing the activation of TLR9 in a cell, a method of inhibiting or suppressing the activation of TLR9 in a cell using such aptamer molecules, a pharmaceutical composition and a kit comprising such aptamer molecules and the use of aptamer molecules for inhibiting or suppressing TLR9 activation. | 2019-12-26 |
20190390200 | RNA-BASED COMPOSITIONS AND ADJUVANTS FOR PROPHYLACTIC AND THERAPEUTIC TREATMENT - The present invention is directed towards an artificial RNA nanostructure comprising multiple external strands of RNA, each external strand comprising about 40-50 nucleotides; one internal strand of RNA comprising more than about 50 nucleotides; the internal strands and external strands assembled to form a triangle nanostructure, a square nanostructure, or a polygon nanostructure and a pRNA three-way junction (3WJ) motif at each vertex of the nanostructure. Such nanostructure can be provided in a composition together with an adjuvant for use in inducing the production of high affinity neutralizing antibodies or inhibitory antibodies, inducing the production of cytokines, inducing an immune response in a subject, or a combination thereof. | 2019-12-26 |
20190390201 | INTERFERING RNA MOLECULES - The present invention is related to a ribonucleic acid comprising a double stranded structure whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and whereby said first stretch is at least partially complementary to a target nucleic acid, and the second grand comprises a second stretch of contiguous nucleotides whereby said second stretch is at least partially identical to a target nucleic acid, and whereby the double stranded structure is blunt ended. | 2019-12-26 |
20190390202 | METHODS AND COMPOSITIONS OF SHORT SMALL HAIRPIN RNAS AND MICRORNAS FOR WOUND HEALING - Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing. | 2019-12-26 |
20190390203 | REDUCTION OF LIPASE ACTIVITY IN PRODUCT FORMULATIONS - The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof. | 2019-12-26 |
20190390204 | INDUCIBLE DNA BINDING PROTEINS AND GENOME PERTURBATION TOOLS AND APPLICATIONS THEREOF - The present invention generally relates to methods and compositions used for the spatial and temporal control of gene expression that may use inducible transcriptional effectors. The invention particularly relates to inducible methods of altering or perturbing expression of a genomic locus of interest in a cell wherein the genomic locus may be contacted with a non-naturally occurring or engineered composition comprising a deoxyribonucleic acid (DNA) binding polypeptide. | 2019-12-26 |
20190390205 | INCORPORATION OF INTERNAL POLYA-ENCODED POLY-LYSINE SEQUENCE TAGS AND THEIR VARIATIONS FOR THE TUNABLE CONTROL OF PROTEIN SYNTHESIS IN BACTERIAL AND EUKARYOTIC CELLS - The present disclosure relates to modulation of protein expression. | 2019-12-26 |
20190390206 | METHODS FOR GENERATING TRANSGENIC PLANTS - This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette. | 2019-12-26 |
20190390207 | METHOD FOR INTRODUCING SUBSTANCE INTO PLANT - The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises introducing a substance into a plant germ cell with incomplete cell wall formation. | 2019-12-26 |
20190390208 | Methods for Transfecting Plants and for Reducing Random Integration Events - The present disclosure provides methods for transfecting plants and for expressing RNA or polypeptide molecules in plants. In particular, plants having reduced POLQ expression and/or activity are transfected in order to reduce random integration events. The disclosure further provides transfected plants and plant progeny produced by the methods disclosed herein. | 2019-12-26 |
20190390209 | FERTILE TRANSPLASTOMIC LEGUMINOUS PLANTS - The invention relates to the transformation of plastids from plants, and more precisely to the production of fertile transplastomic leguminous plants, in particular of fertile transplastomic soybean. | 2019-12-26 |
20190390210 | METHODS AND COMPOSITIONS FOR EXPRESSION CASSETTES WITH SUBSTITUTED ELEMENTS FOR ENHANCED EXPRESSION - In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO. 9 (tZmUbi1-01), SEQUENCE ID NO. 10 (tZmUbi361-01). | 2019-12-26 |
20190390211 | METHODS AND COMPOSITIONS FOR TARGETING RNA POLYMERASES AND NON-CODING RNA BIOGENESIS TO SPECIFIC LOCI - The present disclosure relates to recombinant proteins that induce epigenetic gene silencing and to methods of using such proteins for reducing the expression of genes in plants. | 2019-12-26 |
20190390212 | GENE IMPACTING CELLULOSE CONTENT AND BIOMASS FORMATION AND METHODS OF USE - The invention relates to expression of the PdHB3 gene and uses thereof. The invention provides genetically engineered plants expressing the PdHB3 gene and inhibitors of PdHB3. The invention further provides a method for biofuel production using plant genetically engineered for expressing the PdHB3 gene. The invention further provides a method for increasing glucose and/or xylose release in a plant or plant cell. | 2019-12-26 |
20190390213 | HAPLOIDIZATION IN SORGHUM - Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants. | 2019-12-26 |
20190390214 | Disruption of ROCK1 gene leads to plants with improved traits - The present invention provides a method for improving traits in a plant, like e.g. improving yield-related traits like number of flowers, number of siliques, seed yield, stem growth in a plant, the method comprising disruption of endogenous ROCK1 gene in a plant cell, wherein said disruption inhibits expression and/or activity of a product of said endogenous ROCK1 gene compared to a corresponding control plant cell lacking such a disruption. | 2019-12-26 |
20190390215 | OILS, LIPIDS AND FATTY ACIDS PRODUCED IN TRANSGENIC BRASSICA PLANT - The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant. | 2019-12-26 |
20190390216 | p-Coumaroyl-CoA:Monolignol Transferase - The invention relates to nucleic acids encoding a p-coumaroyl-CoA:monolignol transferase and to inhibitory nucleic acids adapted to inhibit the expression and/or translation of a p-coumaroyl-CoA:monolignol transferase RNA. Inhibition of p-coumaroyl-CoA:monolignol transferase in plants improves the incorporation of monolignol ferulates into the lignin of plants, giving rise to plant biomass that is more easily processed into useful products such as paper and biofuels. | 2019-12-26 |
20190390217 | PHARMACEUTICAL PROTEINS, HUMAN THERAPEUTICS, HUMAN SERUM ALBUMIN INSULIN, NATIVE CHOLERA TOXIN B SUBUNIT ON TRANSGENIC PLASTIDS - This invention relates in part to synthesizing high value pharmaceutical proteins in transgenic plants by chloroplast expression for pharmaceutical protein production. We use poly(GVGVP), for example, as a fusion protein to enable hyper-expression of insulin and to accomplish rapid one step purification of fusion peptides utilizing the inverse temperature transition properties of this polymer. We also use insulin-CTB fusion protein in chloroplasts of nicotine free edible tobacco (LAMD 605) for oral delivery. This invention includes expression of native cholera toxin B subunit gene as oligomers in transgenic tobacco chloroplasts which may be utilized in connection with large-scale production of purified CTB, as well as an edible vaccine if expressed in an edible plant, as a transmucosal carrier of peptides to which it is fused to enhance mucosal immunity, and/or to induce oral tolerance of the products of these peptides. The present invention also relates in part to recombinant DNA vectors for enhanced expression of human serum albumin, insulin-like growth factor 1, and interferon-α 2 and 5, via chloroplast genomes. | 2019-12-26 |
20190390218 | Fungal Resistant Plants Expressing RLK1 - The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae,in plants and/or plant cells. This is achieved by increasing the expression of an RLK1protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK1protein. | 2019-12-26 |
20190390219 | INSECTICIDAL COMBINATIONS OF PLANT DERIVED INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE - Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding a silencing element and an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, Hemipteran, fungi and nematode pest populations and for producing compositions with insecticidal activity. | 2019-12-26 |
20190390220 | Compositions and Methods of Increasing Aphid Resistance in Transgenic Plants - The present invention relates to isolated resistance genes encoding polypeptides that confers resistance to aphid infestation, and methods of transforming plant and cells with the disclosed resistance genes. The invention also relates to transgenic plants and cells having resistance to aphids, particularly | 2019-12-26 |
20190390221 | PLASMID VECTORS FOR EXPRESSION OF LARGE NUCLEIC ACID TRANSGENES - Provided herein, in certain embodiments, are plasmid expression vectors and methods of use of such vectors for either transient or stable integrated expression of transgenes in eukaryotic cells. The plasmid expression vectors provided herein are less than 3.6 kb in size and can accommodate large (>5 kb) polynucleotide insertions of transgenes and homology arms for stable integration. | 2019-12-26 |
20190390222 | SYSTEMS AND METHODS FOR IN VIVO DUAL RECOMBINASE-MEDIATED CASSETTE EXCHANGE (dRMCE) AND DISEASE MODELS THEREOF - Described herein are donor vectors and systems for use in in vivo dual recombinase-mediated cassette exchange. Also described are animal models for consistent, rigorous, and facile investigation of transgene expression. Further described are methods of screening for therapeutic drugs using these animal models, and methods of treatment | 2019-12-26 |
20190390223 | NOVEL CAS9 PROTEINS AND GUIDING FEATURES FOR DNA TARGETING AND GENOME EDITING - The present invention is directed to methods and compositions comprising novel CRISPR polypeptides and polynucleotides for site-specific cleavage and nicking of nucleic acids, transcriptional control and genome editing. | 2019-12-26 |
20190390224 | ACID-ALPHA GLUCOSIDASE VARIANTS AND USES THEREOF - The present invention relates to variants of acid-alpha glucosidase and uses thereof. | 2019-12-26 |
20190390225 | ACID-ALPHA GLUCOSIDASE VARIANTS AND USES THEREOF - The present invention relates to variants of acid-alpha glucosidase and uses thereof. | 2019-12-26 |
20190390226 | NUCLEIC ACID-GUIDED NUCLEASES - Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods. | 2019-12-26 |
20190390227 | CRISPR ENABLED MULTIPLEXED GENOME ENGINEERING - Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used. | 2019-12-26 |
20190390228 | SEC Modified Strains for Improved Secretion of Recombinant Proteins - Disclosed herein are modified strains for improving secretion of recombinantly expressed products secreted from a host organism with improved growth and productivity characteristics, as well as methods of using the modified strains. | 2019-12-26 |
20190390229 | GENE EDITING REAGENTS WITH REDUCED TOXICITY - The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents. Further disclosed are nucleic acid compositions with reduced cytotoxicity. | 2019-12-26 |
20190390230 | MULTIFUNCTIONAL ALLELES - Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation. | 2019-12-26 |
20190390231 | METHOD FOR THE MICROBIAL PRODUCTION OF 8-METHYL NONANOIC ACID - The present disclosure relates to the production of 8-methyl nonanoic acid and medium chain branched fatty acids, e.g., via microbial fermentation. | 2019-12-26 |
20190390232 | COMPOSITIONS AND METHODS FOR THE PRODUCTION OF PYRUVIC ACID AND RELATED PRODUCTS USING DYNAMIC METABOLIC CONTROL - The present disclosure is related to genetically engineered microbial strains and related bioprocesses for the production of pyruvate and related products. Specifically, the use of dynamically controlled synthetic metabolic valves to reduce the activity of enzymes known to contribute to pyruvate synthesis, leads to increased pyruvate production in a two-stage process rather than a decrease in production. | 2019-12-26 |
20190390233 | Processes and Systems for Catalytic Manufacture of Wax Ester Derivatives - Processes for transesterifying wax esters. Implementations may include providing a feedstock including wax esters, introducing into the feedstock an alcohol with a carbon number ranging from 1 to 34 carbons where the alcohol is selected from the group consisting of a straight chain alcohol, a branched chain alcohol, any combination of straight chain alcohols, any combination of branched chain alcohols, and any combination thereof. The process may include contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. The enzymatically transesterified product may be adapted to produce a finished product having a certain formula that has a viscosity that substantially matches a viscosity of a finished product having the same certain formula including chemically catalyzed transesterified wax esters. | 2019-12-26 |
20190390234 | Processes and Systems for Catalytic Manufacture of Wax Ester Derivatives - Processes for transesterifying wax esters. Implementations may include providing a feedstock including wax esters, introducing into the feedstock an alcohol with a carbon number ranging from 1 to 34 carbons where the alcohol is selected from the group consisting of a straight chain alcohol, a branched chain alcohol, any combination of straight chain alcohols, any combination of branched chain alcohols, and any combination thereof. The process may include contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. The enzymatically transesterified product may be adapted to produce a finished product having a certain formula that has a viscosity that substantially matches a viscosity of a finished product having the same certain formula including chemically catalyzed transesterified wax esters. | 2019-12-26 |
20190390235 | Processes for the Preparation of Dasotraline and Intermediates Thereof - The present invention provides processes for the preparation of Dasotraline, as well as intermediates useful in the preparation thereof. In particular, processes are provided for the enzymatic transamination of a compound of Formula (2) to afford Dasotraline. | 2019-12-26 |
20190390236 | ENGINEERED BIOSYNTHETIC PATHWAYS FOR PRODUCTION OF TYRAMINE BY FERMENTATION - The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods. | 2019-12-26 |
20190390237 | NEPETALACTOL OXIDOREDUCTASES, NEPETALACTOL SYNTHASES, AND MICROBES CAPABLE OF PRODUCING NEPETALACTONE - The present disclosure provides isolated nepetalactone oxidoreductase polypeptides (NORs), nepetalactol synthases (NEPSs), and related polynucleotides, engineered host cells, and cultures, as well as methods for producing NORs and NEPSs, and for using them to produce nepetalactol, nepetalactone, and dihydronepetalactone. The present disclosure also provides methods for engineering cells (e.g., microbial cells) to produce nepetalactone from a fermentation substrate such as glucose, as well as engineered cells having this capability and related cultures and methods for producing nepetalactone. | 2019-12-26 |
20190390238 | METHOD FOR PRODUCING N-ACETYL-D-GLUCOSAMINE AND/OR D-GLUCOSAMINE SALT BY MICROBIAL FERMENTATION - The present invention discloses a process for producing N-acetyl-D-glucosamine and D-glucosamine salts by microbial fermentation. The invention includes a method to produce N-acetyl-D-glucosamine and/or D-glucosamine salts with higher efficiency and higher yield by increasing the effect of N-acetyl-D-aminomannose-6-phosphate epimerase in microorganisms. | 2019-12-26 |
20190390239 | TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS - Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as | 2019-12-26 |
20190390240 | Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same - The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided. | 2019-12-26 |
20190390241 | VIRAL RESISTANT CELLS AND CULTURE SYSTEMS - Mammalian cell lines genetically engineered to be viral resistant, cell culture systems comprising agents that inhibit viral entry into or translocation within cells, and methods of using said cell lines and/or said cell culture systems to reduce or prevent viral contamination of biologic production systems. | 2019-12-26 |
20190390242 | 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE MUTANTS AND PROCESS FOR THE PREPARATION OF URSODEOXYCHOLIC ACID - The invention provides novel 3α-hydroxysteroid dehydrogenase mutants, sequences that code for these enzyme mutants, methods for producing the enzyme mutants, and o the use thereof in enzymatic reactions of cholic acid compounds, and in particular in the production of ursodeoxycholic acid (UDCA). The invention further provides processes for the synthesis of UDCA using the enzyme mutants and the production of UDCA using recombinant microorganisms that have been subjected to multiple modifications. | 2019-12-26 |
20190390243 | BIOMIMETIC VIRUS-BASED COLORIMETRIC SENSORS - The present invention provides, inter alia, a device comprising a colorimetric detection layer configured to undergo a color change upon interaction with a first analyte of interest. The detection layer comprises a first plurality of self-assembled fiber bundles. At least a fraction of the fiber bundles undergo a change from a first conformation to a second conformation upon interaction with the first analyte of interest, thereby undergoing a color change. The invention also provides a method for using the system to detect an analyte of interest. | 2019-12-26 |
20190390244 | METHOD FOR DETERMINING WHETHER OR NOT SAMPLE CONTAINS CERCOSPORA FUNGUS OR PSEUDOCERCOSPORA FUNGUS - The present invention provides a method for determining whether or not a sample contains at least one of | 2019-12-26 |
20190390245 | CYTOTOXICITY TEST METHOD FOR MEDICAL DEVICE - A new two-layer modified agar overlay cytotoxicity method for medical device safety assessment are developed. The new two layer modified agar overlay cytotoxicity method has equivalent sensitivity, and greater efficiency compared to the ISO/USP direct contact and agar overlay assays. Assays were carried out in 6-well microplates. Nutrient agar medium with a 0.5% agar concentration was used to provide a base nutrient layer to support cell growth, L929 cells mixed nutrient agar (0.33% agar) were seeded on top of the base agar and cytotoxicity was evaluated after 24 hours per USP. Results demonstrated the two layer modified agar overlay cytotoxicity assay performs as well as the ISO/USP direct contact method with distinct advantages. Results showed that this two layer modified agar overlay method is more promising as compared to the traditional agar overlay and direct contact methods due to the diluted soft agar, avoided potential mechanical damage from test materials, and represents a valuable tool to evaluate medical device cytotoxicity. | 2019-12-26 |
20190390246 | METHODS, APPARATUSES AND SYSTEMS FOR ANALYZING MICROORGANISM STRAINS FROM COMPLEX HETEROGENEOUS COMMUNITIES, PREDICTING AND IDENTIFYING FUNCTIONAL RELATIONSHIPS AND INTERACTIONS THEREOF, SELECTING AND SYNTHESIZING ENDOMICROBIAL ENSEMBLES BASED THEREON, AND ENDOMICROBIAL ENSEMBLE SUPPLEMENTS AND SUPPLEMENTATION - Methods and systems for identifying and/or forming a synthetic ensemble, synthetic bioensemble, and/or Endomicrobial Supplement (EMS) are disclosed. Methods of making synthetic microbial ensembles to inhibit bacterial fowl pathogen colonization in the gastrointestinal tract of fowl are disclosed. Method for modulating the alpha and/or beta diversity in the microbial population of the gastrointestinal tract of fowl is disclosed | 2019-12-26 |
20190390247 | PQQ-sGDH MUTANT, POLYNUCLEOTIDE AND GLUCOSE DETECTION BIOSENSOR - The present invention provides a soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-sGDH) mutant, wherein the amino acid positions thereof correspond to a wild-type PQQ-sGDH sequence of | 2019-12-26 |
20190390248 | SERUM PREPARATION - This invention relates to the use of clotting compositions containing prothrombin activators to produce high quality blood serum samples for pathology and other biological assays, and to containers containing such clotting compositions, and related methods of use. | 2019-12-26 |
20190390249 | BIOLOGICAL SPECIMEN COLLECTION AND TRANSPORT SYSTEM - Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis. | 2019-12-26 |
20190390250 | FRAGMENTATION OF CHAINS OF NUCLEIC ACIDS - Disclosed are methods and devices for fragmenting chains of nucleic acids (such as DNA) in a liquid sample. A liquid sample is provided, comprising chains of nucleic acids. A sample treatment device has a sample treatment zone. The liquid sample is contacted with the sample treatment zone. Surface acoustic waves (SAWs) are propagated along a surface of the sample treatment zone, or more generated acoustic waves are propagated to couple with the sample, and/or the sample is subjected to freeze-thaw cycling, in order to cause fragmentation of said chains of nucleic acids in the sample. | 2019-12-26 |
20190390251 | METHODS OF PRODUCING NUCLEIC ACID LIBRARIES AND COMPOSITIONS AND KITS FOR PRACTICING SAME - Aspects of the present disclosure include methods of producing nucleic acid libraries. In certain aspects, the methods include producing tagged primer extension products, and contacting aliquots of the tagged primer extension products with transposomes to produce tagged extension product fragments. The methods may further include sequencing the tagged extension products and tagged extension product fragments to determine the sequences of nucleic acids of interest. Also provided are compositions and kits that find use, e.g., in practicing embodiments of the methods. | 2019-12-26 |
20190390252 | SYSTEMS AND METHODS FOR PRE-ANALYTICAL SUBSTRATE PROCESSING - Some embodiments presented in this disclosure concern an Automated Tissue Dissection (ATD) System. An ATD system is a one stop, and potentially low-cost, system to perform dissections on a substrate from pathologist digital mark or pen mark on the substrate using non-contact and/or mechanical method to extract a Formalin-Fixed Paraffin-Embedded (FFPE) tissue sample with: (a) only the ROI or ROIs as area to be saved; and (b) remove or decompose nucleic acid content in the region of no interest (RONI) and collect all tissue sample from a standard microscope substrate into a specific container. | 2019-12-26 |
20190390253 | METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID MOLECULES - The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis. | 2019-12-26 |
20190390254 | METHOD FOR NORMALIZING A NUCLEIC ACID SAMPLE - Among other things, a method for normalizing a sample is provided. In some embodiments, the method comprises: (a) reacting a sample with a limiting amount of a single-turnover sequence-specific endonuclease that recognizes a target sequence, thereby cleaving a portion of the nucleic acid molecules that comprise the target sequence and producing a normalized amount of a first cleavage product; and (b) isolating, transcribing or selectively amplifying the normalized amount of the first cleavage product. In this method, because a limiting amount of the endonuclease is used, the normalized amount of the first cleavage product is determined by the limiting amount of the first single-turnover sequence-specific endonuclease used in step (a). | 2019-12-26 |
20190390255 | METHOD FOR PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES - Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. | 2019-12-26 |
20190390256 | Diagnostic Methods and Marker for Bacterial Vaginosis - A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample | 2019-12-26 |
20190390257 | TISSUE-SPECIFIC METHYLATION MARKER - Provided herein are compositions comprising tissue-specific markers for identifying a tissue of origin of a cell-free nucleic acid, e.g., a cell-free DNA molecule. Also provided herein are methods, compositions, and systems for identifying a tissue of origin of a cell-free nucleic acid by determining an absolute amount of cell-free nucleic acids comprising the tissue-specific marker. Also provided herein are methods, compositions, and systems for detecting a cancer in a tissue of an organism by analyzing tissue-specific markers. | 2019-12-26 |
20190390258 | CHEMICAL COMPOSITIONS AND METHODS OF USING SAME - The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays. | 2019-12-26 |
20190390259 | COMPOSITIONS FOR AND METHODS OF ENRICHING GENETIC MUTANTS FOR DETECTING, DIAGNOSING, AND PROGNOSING CANCER - Compositions for detecting, diagnosing and prognosing cancer in individuals having or suspected of having cancer are provided. Said compositions are used to enrich a nucleic acid target comprising a locus of genetic variation, e.g., single nucleotide polymorphism (SNPs) and variable mutations, such as small insertions, deletions, and replacements (“indels”) within a sample for ease and improved detection. In addition, kits are provided for measuring levels or the presence of SNPs and indels associated with cancer for detecting, diagnosing and prognosing cancer. Furthermore, methods are provided for detecting, diagnosing and prognosing cancer in individuals having or suspected of having cancer comprising determining the enrichment levels and/or presence or absence of the SNPs and indels in a subject. | 2019-12-26 |
20190390260 | METHODS AND COMPOSITIONS RELATING TO DETECTION OF RECOMBINATION AND REARRANGEMENT EVENTS - Described herein are methods and assays for detection of recombination and/or rearrangement events in a cell. In some embodiments, the methods and/or assays relate to Linear Amplification Mediated (LAM)-PCR. In some embodiments, the recombination event is a V(D)J recombination event. | 2019-12-26 |
20190390261 | SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS - The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation. | 2019-12-26 |
20190390262 | LOCUS SPECIFIC AMPLIFICATION USING ARRAY PROBES - Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. In preferred aspects the analysis is a determination of sequence at one or more locations in the amplified target. The methods may be used for genotyping, sequencing and analysis of copy number. | 2019-12-26 |