Patent application number | Description | Published |
20130170026 | Convex Lens-Induced Confinement for Measuring Distributions of Molecular Size - A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide. | 07-04-2013 |
20130224756 | OPTOGENETIC PROBES FOR MEASURING MEMBRANE POTENTIAL - The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell. | 08-29-2013 |
20140295413 | SYSTEMS, METHODS, AND WORKFLOWS FOR OPTOGENETICS ANALYSIS - The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology. | 10-02-2014 |
20150177150 | OPTICAL SYSTEM AND ASSAY CHIP FOR PROBING, DETECTING AND ANALYZING MOLECULES - Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors. | 06-25-2015 |
20150223679 | BLUE LIGHT-ACTIVATED ION CHANNEL MOLECULES AND USES THEREOF - The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function. | 08-13-2015 |
20150285820 | OPTOGENETIC PROBES FOR MEMBRANE POTENTIAL - The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell. | 10-08-2015 |
20150293336 | Spatial Filter Enhanced Spinning Disk Confocal Microscope - A spatial filter includes a first focal plane to receive sample fluorescence and auto-fluorescence from a microscope, a first lens to receive the sample fluorescence and auto-fluorescence and focus rays of the sample fluorescence, a mask aperture positioned in a plane where sample fluorescence rays maximally converge, the mask aperture positioned where such rays converge to pass the rays, the aperture having a size that is a function of characteristics of the microscope, and a second lens positioned to receive the passed rays from the spatial filter and form images at a second focal plane to couple to a camera. | 10-15-2015 |
20150369740 | OPTOGENETIC PROBES FOR MEASURING MEMBRANE POTENTIAL - Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants. | 12-24-2015 |