Gasdaska
Charles Gasdaska, Shrewsbury, MA US
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20140102358 | METHOD, DIE, AND APPARATUS FOR CRYSTAL GROWTH - An apparatus, die, and method can be used form a ribbon from a melt, where capillaries are relatively short and spacers are relatively long as compared to a die opening. Such a configuration can cause the melt to flow is a transverse direction that is substantially parallel to the solid/liquid interface to help move impurities to desired locations. In a particular embodiment, a crystal ribbon can be formed where defects, such as microvoids and impurities, are at higher concentrations near outer edges of the crystal ribbon. The outer edges can be removed to produce crystal substrates that are substantially free of microvoids and have no or a relatively low concentration of impurities. In another particular embodiment, the transverse flow can also help to increase the crystal growth rate. | 04-17-2014 |
Charles J. Gasdaska, Shrewsbury, MA US
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20160090663 | METHOD OF INCLUDING DEADSORPTION AND CRYSTAL GROWTH - A method can include deadsorbing an impurity from an initial material to form a deadsorbed material, melting the deadsorbed material to form a melt within the crucible, and growing a crystal from the melt. In an embodiment, growing is performed at a growth rate that is at least 1.1 times a growth rate of a different crystal formed from a melt of the initial material using a same crystal growth technique, having a same cross-sectional shape, size, and crystal orientation, and a same haze level. In another embodiment, the method can include crushing an initial material to reduce closed porosity before or during deadsorbing impurities. | 03-31-2016 |
20160090667 | FEED SYSTEM INCLUDING A DEADSORPTION UNIT AND A TUBE AND A METHOD OF USING THE SAME - A feed system for a crystal growth apparatus can include a deadsorption unit and a tube. In an embodiment, the deadsorption unit can deadsorb an impurity from a material used to form a crystal. The tube can be fluidly coupled to the deadsorption unit and the crystal growth apparatus to transfer the material from a lower point to a higher point. In another embodiment, any finite number of deadsorption units may be coupled to any finite number of crystal growth apparatuses. In a further embodiment, a crystal growth system can include the feed system and a crystal growth apparatus, wherein the feed system can continuously provide crystal-forming material to the crystal growth apparatus as a crystal is being formed. | 03-31-2016 |
John Gasdaska, Carrboro, NC US
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20090282584 | EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED - Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. | 11-12-2009 |
20120258491 | EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED - Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. | 10-11-2012 |
John Gasdaska, Chapel Hill, NC US
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20110250177 | ALPHA INTERFERON VARIANTS - The present invention provides biologically active variants of human α-2b-interferon. The variants contain carboxy terminus truncations when compared with the amino acid sequence of full-length human α-2b-interferon. It is the novel finding of the present invention that these truncated variants have the biological activity of full-length human α-2b-interferon. The invention encompasses these biologically active variant α-interferons, as well as polynucleotides encoding these interferons. Expression cassettes comprising these polynucleotides and host cells comprising the expression cassettes are also provided. The invention also provides compositions comprising variant α-interferon polypeptides and a pharmaceutically acceptable carrier. | 10-13-2011 |
John R. Gasdaska, Carrboro, NC US
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20100186126 | EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED - The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed. | 07-22-2010 |
20120220015 | COMPOSITIONS AND METHODS FOR PRODUCTION OF AGLYCOSYLATED PLASMINOGEN - Compositions and methods for producing aglycosylated plasminogen (PLG) polypeptides and fragments and variants thereof are provided. Compositions of the invention include isolated nucleic acid molecules encoding aglycosylated PLG polypeptides in which the asparagine (Asn) residue corresponding to residue Asn-289 of the mature human PLG polypeptide has been substituted with an amino acid residue that does not support N-linked glycosylation at that position of the PLG polypeptide, as well as the aglycosylated PLG polypeptides encoded thereby. Expression constructs comprising these PLG-encoding nucleic acid molecules and transgenic plants comprising these expression constructs are also provided. Methods of the invention comprise introducing a PLG-encoding nucleic acid molecule of the invention into a plant of interest and culturing the plant under conditions to produce the aglycosylated PLG polypeptide. The aglycosylated PLG polypeptide allows for significant increases in production and yield of PLG from a plant-based expression system without comprising the ability of the PLG product to be activated to a polypeptide capable of binding fibrin and having serine protease activity, including biologically active plasmin that is also glycosylated. The activated aglycosylated plasmin is useful to treat diseases or conditions associated with a thrombus. | 08-30-2012 |