Patent application number | Description | Published |
20090197284 | Analysis of Proteins from Biological Fluids Using Mass Spectrometric Immunoassay - Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states. | 08-06-2009 |
20100181239 | Integrated High Throughput System for the Analysis of Biomolecules - Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. | 07-22-2010 |
20100267069 | ANALYSIS OF PROTEINS FROM BIOLOGICAL FLUIDS USING MASS SPECTROMETRIC IMMUNOASSAY - Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states. | 10-21-2010 |
20100267581 | HIGH THROUGHPUT INTEGRATED MICROFLUIDIC SYSTEM AND DEVICE - A microfluidic system and device capable of high throughput which includes a multi-channel micro-column having a high surface area material having at least one affinity reagent bound thereto contained within a housing. The structure of the housing and/or the multi-channel micro-column is of a sufficient size and configuration to enable capture and purification of an analyte contained within a sample and one or more of binding of a reporter molecule to the analyte and a substrate chemical reaction with the analyte to all take place within the housing and/or the multi-channel micro-column. | 10-21-2010 |
20100330539 | PERIODONTAL TREATMENT SYSTEM AND METHOD - Methods and apparatuses for treating a root canal in a tooth or hard and/or soft tissue within a tooth and surrounding tissues by pulsing a laser light into a reservoir, preferably after introducing liquid fluid into the reservoir, so as to disintegrate, separate, or otherwise neutralize pulp, plaque, calculus, and/or bacteria within and adjacent the fluid reservoir without elevating the temperature of any of the dentin, tooth, bones, gums, other soft tissues, other hard tissues, and any other adjacent tissue more than about 5° C. | 12-30-2010 |
20110192778 | INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES - Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. | 08-11-2011 |
20110269099 | DENTAL AND MEDICAL TREATMENTS AND PROCEDURES - A method treating a root canal in a tooth by introducing into the pulp chamber of a tooth and pulsing a laser light into the fluid reservoir so as to disintegrate pulp within the root canal without generation of any significant heat in said liquid fluid so as to avoid elevating the temperature of any of the dentin, tooth, or other adjacent tissue more than about 5° C. | 11-03-2011 |
20120085692 | INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES - Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. | 04-12-2012 |
20130143180 | PERIODONTAL TREATMENT SYSTEM AND METHOD - Methods and apparatuses for treating a root canal in a tooth or hard and/or soft tissue within a tooth and surrounding tissues by pulsing a laser light into a reservoir, preferably after introducing liquid fluid into the reservoir, so as to disintegrate, separate, or otherwise neutralize pulp, plaque, calculus, and/or bacteria within and adjacent the fluid reservoir without elevating the temperature of any of the dentin, tooth, bones, gums, other soft tissues, other hard tissues, and any other adjacent tissue more than about 5° C. | 06-06-2013 |
20140004020 | STAND FOR USE WITH AFFINITY CAPTURE | 01-02-2014 |
20140087333 | PERIODONTAL TREATMENT SYSTEM AND METHOD - Methods and apparatuses for treating a root canal in a tooth or hard and/or soft tissue within a tooth and surrounding tissues by pulsing a laser light into a reservoir, preferably after introducing liquid fluid into the reservoir, so as to disintegrate, separate, or otherwise neutralize pulp, plaque, calculus, and/or bacteria within and adjacent the fluid reservoir without elevating the temperature of any of the dentin, tooth, bones, gums, other soft tissues, other hard tissues, and any other adjacent tissue more than about 5° C. | 03-27-2014 |
20140220511 | PERIODONTAL TREATMENT SYSTEM AND METHOD - The specification discloses embodiments and other information concerning methods, systems, and apparatus in accordance with aspects of the subject invention for treating diseased root canals in teeth and other dental condition, such as periodontitis and the like wherein a laser light is pulsed from a laser fiber optic tip into liquid contained in a reservoir or other space within the canal, periodontal sulcus/pocket, or other dental space immersing the area in need of treatment so as to disintegrate, separate, or otherwise neutralize pulp, plaque, calculus, bacteria, and other microbes, thus annihilating and particleizing undesirable material within the liquid in the reservoir by the action of relatively intense, photoacoustic micro-pressure waves propagating throughout the liquid as a result of the laser light pulses emitted into the liquid from the tip, thereby enabling easy removal of the particles by finely dispersing the same in the liquid and then flushing the particle-laden liquid from the area by hydraulic swirling actions associated with the pulsing light and through ensuing antiseptic rinses, all without elevating the temperature of nearby dentin, tooth, bones, gums, and/or other soft/hard adjacent tissues more than about 5° C. so as to avoid or eliminate harmful effects of localized heating of the same. | 08-07-2014 |