Kleinfeld, US
Alan Kleinfeld, La Jolla, CA US
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20100292086 | METHOD OF SCREENING FOR METABOLITE TRANSPORT MODIFIERS - Methods for discovering compounds that modulate metabolite transport across cell membranes are disclosed. Fluorescently labeled protein probes which have an affinity for an unbound metabolite are trapped in cells. The influx of the unbound metabolite into the cell causes a change in the fluorescence of the protein probe. The ability of test compounds to modulate the fluorescent signal is used to screen for compounds which affect transport of the unbound metabolite. In an example, the unbound metabolite is an unbound free fatty acid and the probe is a fatty acid binding protein. | 11-18-2010 |
Alan Marc Kleinfeld, La Jolla, CA US
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20090318299 | DEVELOPMENT AND USE OF FLUORESCENT PROBES OF UNBOUND ANALYTES - A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer. | 12-24-2009 |
20100062948 | USE OF PROBES FOR UNBOUND METABOLITES - Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction. | 03-11-2010 |
20100298162 | DEVELOPMENT AND USE OF CYSTEINE-LABELED FLUORESCENT PROBES OF UNBOUND ANALYTES - A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease. | 11-25-2010 |
20150044692 | DEVELOPMENT AND USE OF FLUORESCENT PROBES OF UNBOUND BILIRUBIN - Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample. | 02-12-2015 |
Brian J. Kleinfeld, Saginaw, MI US
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20110059803 | Constant Velocity Joint - A boot assembly and constant velocity joint utilizing the boot assembly is provided. The constant velocity joint and boot assembly includes a first shaft, the first shaft having an axle portion extending between a first end and a second end, at least one of the first end and the second end having an enlarged joint portion. It also includes a hollow boot disposed on the first shaft having a joint end and a shaft end, the joint end disposed proximate the enlarged joint portion and the shaft end disposed proximate the axle portion. The constant velocity joint also includes a spacer, the spacer having an inner surface disposed on the axle portion and an outer surface having the shaft end of the boot disposed thereon. | 03-10-2011 |
Chris Kleinfeld, Olivet, MI US
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20110072807 | Combined Exhaust Gas Aftertreatment/Air Cleaner Dust and Ejector Unit - A combined exhaust gas aftertreatment/dust ejector unit ( | 03-31-2011 |
Christopher J. Kleinfeld, Olivet, MI US
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20110252788 | Bypass Fluid System For Exhaust Aftertreatment - An exhaust treatment system for a vehicle includes an intake conduit adapted to receive pressurized intake air provided from a turbocharger compressor and supply the pressurized intake air to an internal combustion engine. An uninterrupted bypass conduit bypasses the internal combustion engine and includes upstream and downstream ends. The upstream end branches from the intake conduit. An exhaust conduit includes an upstream end adapted to receive exhaust from the internal combustion engine. An upstream end of a mixing tube is fixed to and in fluid communication with a downstream end of the exhaust conduit and the downstream end of the bypass conduit at a tubular joint, wherein the exhaust and the pressurized intake air are mixed within the mixing tube. An exhaust treatment device is in fluid communication with a downstream end of the mixing tube. | 10-20-2011 |
20120090305 | Exhaust Gas Stream Vortex Breaker - An exhaust gas treatment system for reducing emissions from an engine includes an exhaust conduit adapted to supply an exhaust stream from an engine to an exhaust treatment device. An injector injects a reagent through an aperture in the conduit into the exhaust stream. A vortex breaker includes a mount having a cylindrical sleeve extending through the aperture as well as a flared tube fixed to and positioned within the cylindrical sleeve. A window extends through an upstream portion of the cylindrical sleeve exposing the flared tube to the exhaust stream. The sleeve includes a downstream aperture in fluid communication with the window. | 04-19-2012 |
20120272639 | Reductant Quality Sensor - A control system for reducing nitrogen oxides in engine exhaust includes an emissions catalyst having an inlet adapted to receive an exhaust from the engine. A reductant tank is configured to store a reductant. An injector is in fluid communication with the reductant tank and is operable to inject the reductant into the exhaust upstream of the catalyst. A sensor is in fluid communication with the reductant and outputs a reductant quality signal based on the reductant. A controller determines a reductant quality of the reductant based on the reductant quality signal and outputs a dosing rate signal based on the reductant quality. The injector alters a dosing rate of the reductant based on the dosing signal. | 11-01-2012 |
David Kleinfeld, La Jolla, CA US
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20100278746 | Method for Measuring Neurotransmitters in Vivo - A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths. | 11-04-2010 |
20140103018 | Spatially chirped pulses for femtosecond laser ablation through transparent materials - Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates. | 04-17-2014 |
David Kleinfeld, San Diego, CA US
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20110297651 | Spatially chirped pulses for femtosecond laser ablation through transparent materials - Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates. | 12-08-2011 |
Elaine Robbins Kleinfeld, Macungie, PA US
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20150270184 | Location-Shifted Probe Pads For Pre-Bond Testing - An arrangement for performing pre-bond testing of a wafer of semiconductor devices utilizes probe pads that are location-shifted into wafer regions adjacent to the devices such that when the pre-bond testing is completed and the wafer is separated into individual elements, the electrical connection between the pre-bond probe pad and tested device is broken. The adjacent wafer regions may be “vacant” areas or another device region. When separated into individual components, a given pre-bond probe pad and its associated device will be physically separated and electrically isolated from one another. Thus, a large probe pad is electrically connected to an associated device only while the wafer is intact, facilitating probe placement during pre-bond testing. Once the devices are separated, the probe pad is disconnected from its associated active element portion, eliminating the capacitance associated with maintaining an electrical connection between a co-located probe and active region. | 09-24-2015 |