Patent application number | Description | Published |
20100261226 | UNIVERSAL BIOREACTORS AND METHODS OF USE - Bioreactors suitable for housing a predetermined volume of culture medium comprising a plurality of containers of approximately equal internal volume in which the culture medium resides, wherein the predetermined volume of culture medium is retained in approximately equal amounts within each of the plurality of containers during operation of the bioreactor, and wherein the internal volume of each container is selected so that the amount of culture medium in each container exceeds 50 vol. % of the container internal volume during operation of the bioreactor, and related methods of use. | 10-14-2010 |
20110117538 | BIOREACTORS FOR FERMENTATION AND RELATED METHODS - Bioreactors suitable for housing a predetermined volume of liquid comprising nutrient medium and biological culture comprising: (a) a container having at least one interior wall; (b) at least one nutrient medium inlet; (c) at least one liquid outlet; (d) at least one gas inlet; (e) at least one gas outlet; and (f) at least one cylindrical sparging filter attached to the at least one gas inlet, wherein the sparging filter comprises a plurality of pores along its axis which permit gas to be emitted radially from the sparging filter into the liquid, wherein the diameter of the plurality of pores does not exceed about 50 μm, and wherein the orientation of the at least one sparging filter within the container provides for immersion of the plurality of pores within the liquid and substantially uniform distribution of emitted gas throughout the liquid, and related methods of using said bioreactors to prepare various biological products. | 05-19-2011 |
20110198286 | Separative Bioreactor - A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor. | 08-18-2011 |
20110201050 | Gas Scrubbed Perfusion Filter - Fine gas bubbles traveling at fast speed are employed to scrub a hard-surface filter for harvesting liquids from suspensions including those consisting of nutrient media and cell culture, on a continuous basis without clogging the filters. | 08-18-2011 |
20110207170 | Bioreactor Exhaust - An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration. | 08-25-2011 |
20110217690 | Interconnected Bioreactors - A method of combining the content of bioreactors allowing homogeneous mixing in accordance with CFR21 is described that allows processing of variable size batches using a single validated bioreactor is described. | 09-08-2011 |
20110217767 | Single-use Stationary Bioreactors and Mixing Vessels - Stationary bioreactors and mixing vessels fitted with single-use flexible bags utilizing wave hydrodynamic principle are described for use in every type of biological process and products. | 09-08-2011 |
20110287404 | Stationary Bubble Reactors - Reactors that allow mixing and gasification by converting the entire floor of the reactor vessel to a sparge filter is described. | 11-24-2011 |
20110290745 | Concentrator Filter - A method and an apparatus for separating suspended matter from liquid includes a concentrator filter that draws the liquid out of the suspension while the filter kept unblocked by a sparging filter that allows scrubbing of the concentrator filter by gas bubbles. This invention can be used to replace cross-flow filtration and centrifugation in the bioprocess industry and to reduce the volume of suspensions to concentrate the yield of the end product in the chemical industry. | 12-01-2011 |
20120006526 | Single-container manufacturing of biological products - A single-pass HVAC systems to isolate zones and to maintain a required clean air quality standard is provided that operates by producing a positive pressure in all zones, while exhausting on a minimal quantity of air required by law. The zones are kept clean by a recirculating fan filter in each zone. The exhaust air is used to exchange heat with incoming air to conserve energy further. | 01-12-2012 |
20120016113 | BUOYANT PROTEIN HARVESTING DEVICE - A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification. | 01-19-2012 |
20120018380 | PREPARATIVE CHROMATOGRAPHY COLUMN AND METHODS - A chromatography column that captures components in a process liquid in a free flow state and allows elution in steps is described. | 01-26-2012 |
20120040331 | Noninvasive Bioreactor Monitoring - A pair or receptacles capable of housing an emitter probe and a detector probe are installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media. | 02-16-2012 |
20120077243 | Closed Bioreactor - Single-use closed bioreactors with recirculating exhaust gas that can be operated in an uncontrolled environment are reported for the manufacturing of biological products using genetically modified biological cultures that produces carbon dioxide or that requires carbon dioxide in their metabolic process. | 03-29-2012 |
20120142047 | Purification and Separation Treatment Assembly (PASTA) for Biological Products - An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns. | 06-07-2012 |
20120149885 | DOWNSTREAM BIOPROCESSING DEVICE - Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing. | 06-14-2012 |
20120152862 | Non-blocking Filter System - A non-blocking filtration system wherein the suspension filtered continuously scrubs the filter, keeping it free of deposits of solid deposits while the filtrate is removed in a variety of biological and chemical applications, substantially reducing the cost of unit operations. | 06-21-2012 |
20120164300 | Accelerated Aging of Wines and Sprits - A method and a system for aging wines and spirits is disclosed using finely pulverized wood of less than 1 mm size and in such quantity to achieve equivalent aging in one-tenth to one-hundredth of the time required for traditional barrel aging and for instant aging prior to drinking. | 06-28-2012 |
20120198600 | WINDY CITY HAT - A felt-hat that does not fly off in the wind having vents in the brim camouflaged by a porous or solid material is disclosed. | 08-09-2012 |
20120231504 | MULTIUSE REACTORS AND RELATED METHODS - A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation. | 09-13-2012 |
20120252108 | PNEUMATICALLY AGITATED AND AERATED SINGLE-USE BIOREACTOR - A single-use round flexible mixing bag for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element comprising a radial flow impeller driven by an internal pneumatic vane motor is disclosed. The bag may include an integral sparger and sensor receiver. Related methods are also disclosed. | 10-04-2012 |
20120258519 | Protein Harvesting - Methods of harvesting proteins directly from bioreactors to avoid at several steps in the purification of recombinant drugs are disclosed. | 10-11-2012 |
20130143313 | SEPARATIVE HARVESTING DEVICE - A harvesting device for capturing a biological product directly by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction, thus allowing one to combine the steps of recombinant expression and separation of a biological product. The method allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor. | 06-06-2013 |
20130171616 | PIVOTING PRESSURIZED SINGLE-USE BIOREACTOR - Pressurized hermetically sealed bags disposed inside a cylindrical support and containing a septum with variable density of porosity and dividing the bag into two chambers are used to provide optimal mixing and gasification of nutrient media to grow a variety of biological cultures, particularly the cell cultures to produce a multitude of pharmaceutical and biotechnology products in a disposable system. | 07-04-2013 |
20130220923 | NON-BLOCKING FILTRATION SYSTEM - A non-blocking filtration system wherein the suspension filtered continuously scrubs the filter, keeping it free of deposits of solid deposits while the filtrate is removed in a variety of biological and chemical applications, substantially reducing the cost of unit operations. | 08-29-2013 |
20130296538 | DOWNSTREAM BIOPROCESSING DEVICE - Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing. | 11-07-2013 |
20130344584 | NON-INVASIVE BIOREACTOR MONITORING - A pair or receptacles capable of housing an emitter probe and a detector probe installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media. | 12-26-2013 |
20140024105 | Baffled Single Use Bioreactor - A flexible disposable bioreactor having three, stagger-baffled compartments wherein the middle compartment houses a sparging rod is described to provide the highest degree of sparging and mixing to produce biological products. | 01-23-2014 |
20140170635 | SEPARATIVE BIOREACTOR - A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor. | 06-19-2014 |
20140175029 | CONCENTRATOR FILTER - A method and an apparatus for separating suspended matter from liquid includes a concentrator filter that draws the liquid out of the suspension while the filter kept unblocked by a sparging filter that allows scrubbing of the concentrator filter by gas bubbles. This invention can be used to replace cross-flow filtration and centrifugation in the bioprocess industry and to reduce the volume of suspensions to concentrate the yield of the end product in the chemical industry. | 06-26-2014 |
20140356968 | METHODS FOR COMPARING A STRUCTURE OF A FIRST BIOMOLECULE AND A SECOND BIOMOLECULE - The present disclosure provides methods to assess structural similarity of a first biomolecule and a second biomolecule by detecting one or more responses of the first and second biomolecule to thermodynamic stress conditions induced by osmotic and dielectric changes including, detecting a shift in fluorescence emission and/or a change in the intensity of the emission. | 12-04-2014 |
20150099293 | BUOYANT PROTEIN HARVESTING DEVICE - A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification. | 04-09-2015 |
20150204834 | THERMODYNAMIC EQUIVALENCE SURROGATE TEST (TEST) FOR BIOEQUIVALENCE - A method for establishing bioequivalence of drug products by comparing the thermodynamic potential of the release of a drug substance from drug products according to a set of statistically differentiated dissolution profiles of an active drug substance contained in the drug product. | 07-23-2015 |
20150210974 | BIOREACTOR EXHAUST - An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration. | 07-30-2015 |
20150253022 | CLEAN ZONE HVAC SYSTEM - A single-pass HVAC systems to isolate zones and to maintain a required clean air quality standard is provided that operates by producing a positive pressure in all zones, while exhausting on a minimal quantity of air required by law. The zones are kept clean by a recirculating fan filter in each zone. The exhaust air is used to exchange heat with incoming air to conserve energy further. | 09-10-2015 |
20150275318 | INTERCONNECTED BIOREACTORS - A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors. | 10-01-2015 |
20150290597 | AERATION DEVICE FOR BIOREACTORS - An aeration and mixing device for disposable flexible bioreactors comprising a mesh of interconnected perforated disposable tubes to form a structure to cover essentially the entire bottom surface of a disposable flexible bioreactor and wherein a continuous flow of gases through the perforations in the tubes provides an aeration and a mixing function. | 10-15-2015 |
20150371120 | VISUAL AXIS OPTIMIZATION FOR ENHANCED READABILITY AND COMPREHENSION - While the printed text is vertically aligned at 90 degrees, the visual axis of the reader is not aligned with the printed or displayed axis of text resulting in difficulties in readability and comprehension of text and images that can be obviated by printing or displaying text and images at an angle to align with the axis of the eye. | 12-24-2015 |
20160083685 | HARVESTING AND PURIFICATION OR PERFUSION YIELDER (HAPPY) DEVICE - A modular device comprising one or more porous substrate subunits comprising a binding substrate that is capable of interacting with a target biological product, either during, or at the end of a manufacturing cycle; and methods of using the device to harvest or purify a biological product. | 03-24-2016 |
20160097073 | PURIFICATION AND SEPARATION TREATMENT ASSEMBLY (PASTA) FOR BIOLOGICALPRODUCTS - An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns. | 04-07-2016 |