Patent application number | Description | Published |
20080227650 | Incrementally Truncated Nucleic Acids and Methods of Making Same - A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology, is also described. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides. | 09-18-2008 |
20080227659 | Incrementally Truncated Nucleic Acids and Methods of Making Same - A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology, is also described. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides. | 09-18-2008 |
20100227374 | PROTEIN STABILIZATION BY DOMAIN INSERTION INTO A THERMOPHILIC PROTEIN - A strategy to improve protein stability by domain insertion. TEM 1 beta-lactamase (BLA) and exo-inulinase, as model target enzymes, are inserted into a hyperthermophilic maltose binding protein from | 09-09-2010 |
20130230925 | MOLECULAR SWITCHES AND METHODS FOR MAKING AND USING THE SAME - The invention provides molecular switches which couple external signals to functionality and to methods of making and using the same. The switches according to the invention can be used, for example, to regulate gene transcription, target drug delivery to specific cells, transport drugs intracellularly, control drug release, provide conditionally active proteins, perform metabolic engineering, and modulate cell signaling pathways. Libraries comprising the switches and expression vectors and host cells for expressing the switches are also provided. | 09-05-2013 |
Patent application number | Description | Published |
20090005266 | Methods for Making and Using Molecular Switches Involving Circular Permutation - The invention provides molecular switches which couple external signals to functionality, and combinatorial methods of making and using the same involving circular permutation of nucleic acid and amino acid sequences. The switches according to the invention can be used, for example, to regulate gene transcription, target drug delivery to specific cells, transport drugs intracellularly, control drug release, provide conditionally active proteins, perform metabolic engineering, and modulate cell signaling pathways. Libraries comprising the switches, expression vectors and host cells for expressing the switches are also provided. | 01-01-2009 |
20140273217 | PRODRUG ACTIVATION IN CANCER CELLS USING MOLECULAR SWITCHES - The present invention features a novel protein engineering strategy by combining the domains of two independent proteins into a molecular switch. The invention features polypeptides comprising a prodrug activating enzyme and a protein that binds a cancer specific marker, polynucleotides encoding the polypeptides, and molecular switches for converting a prodrug into a toxin, comprising the polypeptides. The invention also features methods for converting a prodrug into a toxin, methods for treating cancer, and methods for making the molecular switches, as well as kits. | 09-18-2014 |
Patent application number | Description | Published |
20110135620 | PRODRUG ACTIVATION IN CANCER CELLS USING MOLECULAR SWITCHES - The present invention features a novel protein engineering strategy by combining the domains of two independent proteins into a molecular switch. The invention features polypeptides comprising a prodrug activating enzyme and a protein that binds a cancer specific marker, polynucleotides encoding the polypeptides, and molecular switches for converting a prodrug into a toxin, comprising the polypeptides. The invention also features methods for converting a prodrug into a toxin, methods for treating cancer, and methods for making the molecular switches, as well as kits. | 06-09-2011 |
20130149785 | METHODS FOR MAKING AND USING MOLECULAR SWITCHES INVOLVING CIRCULAR PERMUTATION - The invention provides molecular switches which couple external signals to functionality, and combinatorial methods of making and using the same involving circular permutation of nucleic acid and amino acid sequences. The switches according to the invention can be used, for example, to regulate gene transcription, target drug delivery to specific cells, transport drugs intracellularly, control drug release, provide conditionally active proteins, perform metabolic engineering, and modulate cell signaling pathways. Libraries comprising the switches, expression vectors and host cells for expressing the switches are also provided. | 06-13-2013 |
20130338043 | Efficient, Expansive, User-Defined DNA Mutagenesis - The presently disclosed subject matter provides methods for the creation of one or more user-defined mutations that can be located anywhere in a target sequence, such as in a gene. These mutations can comprise single mutations, multiple mutations, or a comprehensive codon mutagenesis library, in which all possible single codon substitutions in a gene are created. These methods can be used on a single-stranded or double-stranded template. | 12-19-2013 |
20140249302 | METHODS FOR MAKING AND USING MOLECULAR SWITCHES INVOLVING CIRCULAR PERMUTATION - The invention provides molecular switches which couple external signals to functionality, and combinatorial methods of making and using the same involving circular permutation of nucleic acid and amino acid sequences. The switches according to the invention can be used, for example, to regulate gene transcription, target drug delivery to specific cells, transport drugs intracellularly, control drug release, provide conditionally active proteins, perform metabolic engineering, and modulate cell signaling pathways. Libraries comprising the switches, expression vectors and host cells for expressing the switches are also provided. | 09-04-2014 |
Patent application number | Description | Published |
20090237083 | METHOD FOR IMAGING SUBTERRANEAN FORMATIONS - A method for using a tool in a wellbore in a formation to predict an anomaly in the formation ahead of the device, the tool comprising a transmitter for transmitting electromagnetic signals through the formation and a receiver for detecting response signals. the method comprises a) bringing the tool to a first position inside the wellbore; b) energizing the transmitter to propagate a transient electromagnetic signal into the formation; c) detecting a response signal that has propagated through the formation as a result of the transient signal in step (b); d) calculating a derived quantity for the formation as a function of time based on the detected response signal for the formation; e) plotting the derived quantity for the formation against time; f) moving the tool to at least one other position within the wellbore and repeating steps (b) to (e); g) predicting an anomaly in the formation. | 09-24-2009 |
20110006764 | SYSTEM AND METHOD FOR MEASURING A TIME-VARYING MAGNETIC FIELD AND METHOD FOR PRODUCTION OF A HYDROCARBON FLUID - In accordance with the present disclosure, a system and a method are disclosed for measuring a time varying magnetic field. In one aspect, a system comprises a plurality of induction coils arranged to measure the time varying magnetic field using at least one voltage induced in at least one of the induction coils in the plurality of induction coils, a plurality of snubber circuits connected to the plurality of induction coils, each of the snubber circuits of the plurality of snubber circuits arranged to suppress a resonance of a respective one of the induction coils of the plurality of induction coils, and a summing circuit connected to each of the snubber circuits of the plurality of snubber circuits, the summing circuit arranged to sum voltages induced in each of the induction coils in the plurality of induction coils. | 01-13-2011 |
20110036569 | DOWN-HOLE TRANSMITTER SYSTEM, METHOD OF INDUCING A TRANSIENT ELECTROMAGNETIC FIELD IN AN EARTH FORMATION, METHOD OF OBTAINING A TRANSIENT ELECTROMAGNETIC RESPONSE SIGNAL, AND METHOD OF PRODUCING A HYDROCARBON FLUID - A down-hole tool provided with a transmitter system for inducing a transient electromagnetic field in an earth formation has an induction coil with a number of windings to generate essentially a magnetic dipole field. The number of windings is divided into two or more groups of windings arranged to cooperatively generate the essentially magnetic dipole field when energized. Switching means are arranged to essentially simultaneously terminate the energizing of the groups of windings. The groups of windings are electrically isolated from each other or connected in parallel to each other, at least when the energizing is terminated. A transient electromagnetic field may thus be induced in an earth formation by essentially simultaneously terminating energizing each group of windings by operating the switching means. A transient electromagnetic response signal may be recorded, and used in a method of producing a mineral hydrocarbon fluid. | 02-17-2011 |
20110080172 | TRANSMITTER SYSTEM, METHOD OF INDUCING A TRANSIENT ELECTROMAGNETIC FIELD IN AN EARTH FORMATION, METHOD OF OBTAINING A TRANSIENT ELECTROMAGNETIC RESPONSE SIGNAL, AND METHOD OF PRODUCING A HYDROCARBON FLUID - A transmitter system for inducing a transient electromagnetic field in an earth formation comprises an inductive element to generate an electromagnetic field in response to a flow of electric current through the inductive element. Furthermore, switching means arranged to interrupt the flow of electric current through the inductive element, which switching means comprises a primary switch and an auxiliary switch arranged in series connection with each other. The auxiliary switch has a lower breakdown voltage than the primary switch. This induces a transient electromagnetic field in the earth formation. Delay circuitry may impose a time delay between switching of the auxiliary switch relative to switching of the primary switch. A transient electromagnetic response signal may be recorded, and used in a method of producing a mineral hydrocarbon fluid. | 04-07-2011 |
20110166842 | LAYER STRIPPING METHOD - A layer stripping method that quickly and robustly determines values of undetermined parameters that model a formation (F). The layer stripping method includes applying inversion methods to subsets (d) of measured data (d) and corresponding modeled responses such that the value of a parameter determined during the application of an inversion method to one subset of the data (d) and the corresponding modeled response can be used to reduce the inversion space of a modeled response that corresponds to another subset of the data (d), to which an inversion method is subsequently applied. | 07-07-2011 |
Patent application number | Description | Published |
20080291849 | METHOD AND APPARATUS FOR TRANSMITTING EMERGENCY MESSAGES - A method and apparatus for transmitting emergency messages is disclosed. A system that incorporates teachings of the present disclosure may include, for example, a communication device, of a communication system, having a controller element to send a selection of types of emergency alert messages to be transmitted to one or more associates of a subscriber corresponding to the communication device; and receive an emergency alert message of a public emergency alert system for a zone of coverage, wherein the subscriber is in the zone of coverage and wherein the emergency alert message is transmitted to one or more associate communication devices of each of the one or more associates according to the selection of types of emergency alert messages to be transmitted. Additional embodiments are disclosed. | 11-27-2008 |
20120227068 | METHOD AND APPARATUS FOR TRANSMITTING EMERGENCY MESSAGES - A method and apparatus for transmitting emergency messages is disclosed. A system that incorporates teachings of the present disclosure may include, for example, a communication device, of a communication system, having a controller element to send a selection of types of emergency alert messages to be transmitted to one or more associates of a subscriber corresponding to the communication device; and receive an emergency alert message of a public emergency alert system for a zone of coverage, wherein the subscriber is in the zone of coverage and wherein the emergency alert message is transmitted to one or more associate communication devices of each of the one or more associates according to the selection of types of emergency alert messages to be transmitted. Additional embodiments are disclosed. | 09-06-2012 |
20130179919 | METHOD AND APPARATUS FOR TRANSMITTING EMERGENCY MESSAGES - A method and apparatus for transmitting emergency messages is disclosed. A system that incorporates teachings of the present disclosure may include, for example, a communication device, of a communication system, having a controller element to send a selection of types of emergency alert messages to be transmitted to one or more associates of a subscriber corresponding to the communication device; and receive an emergency alert message of a public emergency alert system for a zone of coverage, wherein the subscriber is in the zone of coverage and wherein the emergency alert message is transmitted to one or more associate communication devices of each of the one or more associates according to the selection of types of emergency alert messages to be transmitted. Additional embodiments are disclosed. | 07-11-2013 |
Patent application number | Description | Published |
20140372239 | EVENT-BASED VERSIONING AND VISIBILITY FOR CONTENT RELEASES - The present invention extends to methods, systems, and computer program products for event-based versioning and visibility for content releases. In general, embodiments of the invention categorize content releases, wherein each release can have a different designation, such as, for example, public, limited, limited for down-level clients, etc. Content (e.g., applications, store pages, ratings, reviews, etc.) offered by a content provider (e.g., an online store) can be associated with a specified operating system/store release. Different versions of the same content can potentially be associated with different specified operating system/store releases. When a content release is unveiled, the categorization for the content release can be updated so that the corresponding content is publicly available without changes to the content itself. Accordingly, content can be tied to a system event helping ensure a consistent view of the content and minimizing the number of operations for changing content visibility. | 12-18-2014 |