Patent application number | Description | Published |
20090186396 | ENHANCED PURIFICATION OF PHOSPHORYLATED AND NONPHOSPHORYLATED BIOMOLECULES BY APATITE CHROMATOGRAPHY - Methods are disclosed for the use of apatite chromatography, particularly without reliance upon phosphate gradients, for fractionation or separation of phosphorylated and nonphosphorylated biomolecules. Integration of such methods into multi-step procedures, with other fractionation methods are additionally disclosed. | 07-23-2009 |
20090187005 | ENHANCED PURIFICATION OF ANTIBODIES AND ANTIBODY FRAGMENTS BY APATITE CHROMATOGRAPHY - Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed. | 07-23-2009 |
20090247735 | ENHANCED CAPACITY AND PURIFICATION OF PROTEIN BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications. | 10-01-2009 |
20090270596 | CHROMATOGRAPHY PURIFICATION OF ANTIBODIES - Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step. | 10-29-2009 |
20090318674 | PROCESS FOR PURIFICATION OF ANTIBODIES - The disclosed embodiments are directed to methods and compositions for purification of proteins, in particular, to methods and compositions for an antibody purification process that includes aggregate removal and the use of solubility enhancing additives such as zwitterion-containing compositions to enhance antibody solubility and avoid aggregate formation or occlusion during ion exchange chromatography, yielding a high-purity protein product substantially free of aggregates. | 12-24-2009 |
20100069617 | ENHANCED PROTEIN AGGREGATE REMOVAL BY MIXED MODE CHROMATOGRAPHY ON HYDROPHOBIC INTERACTION MEDIA IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - This invention relates to methods for enhancing purification of proteins such as monoclonal antibodies by chromatography on carboxyl group-containing HIC supports in the presence of zwitterions that are excluded from protein surfaces. In certain embodiments, the invention may permit more effective separation of non-aggregated protein from aggregated protein. | 03-18-2010 |
20100145029 | ENHANCED CAPACITY AND PURIFICATION OF ANTIBODIES BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications. | 06-10-2010 |
20110166332 | ENHANCED ANTIBODY AGGREGATE REMOVAL WITH CAPTO ADHERE IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - The invention relates to a method for separating at least one non-aggregated protein from a liquid protein preparation by contacting said preparation with a multimodal anion exchanger in the presence of protein-excluded zwitterions at a concentration of 0.25 to 2.5 M. | 07-07-2011 |
20110263823 | ENHANCED CAPACITY AND PURIFICATION OF PROTEIN BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications. | 10-27-2011 |
20120264915 | CHROMATOGRAPHY PURIFICATION OF ANTIBODIES - Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step. | 10-18-2012 |
20150024467 | METHODS FOR PURIFICATION OF RECOMBINANT AAV VECTORS - Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used for gene transfer and specifically for gene therapy or vaccination. Recombinant AAV vectors of the invention are substantially free of in-process impurities, including production components such as cellular nucleic acids, cellular proteins, helper virus, and media components. | 01-22-2015 |