Patent application number | Description | Published |
20100112557 | METHOD FOR HIGH RESOLUTION MELT GENOTYPING - Various methods are described that provide for high resolution melt (HRM) genotyping. The embodiments include providing a locus specific primer and two allele specific primers each having a 5′ end with a short tail, providing a nucleic acid having a single nucleotide polymorphism (SNP) base located within 1-20 base pairs of the 3′ end of nucleic acid, hybridizing the locus specific primer and the allele specific primers to the nucleic acid, amplifying the sample using pyrophosphorolysis activated polymerization (PAP) PCR enzyme, and determining the Tm of the amplicons using HRM. In other embodiments, reactions mixtures and kits for HRM genotyping are provided and disclosed. These kits comprise a locus specific primer, one or more allele specific primers each having a 5′ end with a short tail, a nucleic acid, and a pyrophosphorolysis activate polymerization (PAP) PCR enzyme. | 05-06-2010 |
20100112565 | Methods, kits, and reaction mixtures for high resolution melt genotyping - Various methods are described that provide for high resolution melt (HRM) genotyping. Some embodiments comprise providing a locus specific primer, and two allele specific primers each comprising at least one single nucleotide polymorphism (SNP) allele-hybridizable sequence, wherein at least one of the allele specific primers also comprises at least one nucleotide alteration. In some embodiments, a nucleic acid is provided comprising a SNP base located within 1-20 bases of its 3′ end. Some embodiments comprise hybridizing the locus specific primer and at least one of the allele specific primers to the nucleic acid, amplifying the hybridized nucleic acid using pyrophosphorolysis activated polymerization (PAP) PCR, and determining the melting temperature (Tm) of the resulting amplicons, for example, using HRM. In some embodiments, reaction mixtures and kits for HRM genotyping are provided. The reaction mixtures and kits can each comprise a locus specific primer, one or more allele specific primers each comprising at least one SNP allele-hybridizable sequence, and a PAP PCR enzyme, wherein at least one of the allele specific primers also comprises a nucleotide alteration, for example, a tail. | 05-06-2010 |
20100330576 | Method, Kits, and Reaction Mixtures For High Resolution Melt Genotyping - Various methods are described that provide for high resolution melt (HRM) genotyping. Some embodiments comprise providing a locus specific primer, and two allele specific primers each comprising at least one single nucleotide polymorphism (SNP) allele-hybridizable sequence, wherein at least one of the allele specific primers also comprises at least one nucleotide alteration. In some embodiments, a nucleic acid is provided comprising a SNP base located within 1-20 bases of its 3′ end. Some embodiments comprise hybridizing the locus specific primer and at least one of the allele specific primers to the nucleic acid, amplifying the hybridized nucleic acid using pyrophosphorolysis activated polymerization (PAP) PCR, and determining the melting temperature (Tm) of the resulting amplicons, for example, using HRM. In some embodiments, reaction mixtures and kits for HRM genotyping are provided. The reaction mixtures and kits can each comprise a locus specific primer, one or more allele specific primers each comprising at least one SNP allele-hybridizable sequence, and a PAP PCR enzyme, wherein at least one of the allele specific primers also comprises a nucleotide alteration, for example, a tail. | 12-30-2010 |
20110045479 | Method For High Resolution Melt Genotyping - Various methods are described that provide for high resolution melt (HRM) genotyping. The embodiments include providing a locus specific primer and two allele specific primers each having a 5′ end with a short tail, providing a nucleic acid having a single nucleotide polymorphism (SNP) base located within 1-20 base pairs of the 3′ end of nucleic acid, hybridizing the locus specific primer and the allele specific primers to the nucleic acid, amplifying the sample using pyrophosphorolysis activated polymerization (PAP) PCR enzyme, and determining the Tm of the amplicons using HRM. In other embodiments, reactions mixtures and kits for HRM genotyping are provided and disclosed. These kits comprise a locus specific primer, one or more allele specific primers each having a 5′ end with a short tail, a nucleic acid, and a pyrophosphorolysis activate polymerization (PAP) PCR enzyme. | 02-24-2011 |
20110281755 | Karyotyping Assay - This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof. | 11-17-2011 |
20120282606 | METHOD, KITS AND REACTION MIXTURES FOR HIGH RESOLUTION MELT GENOTYPING - Various methods are described that provide for high resolution melt (HRM) genotyping. Some embodiments comprise providing a locus specific primer, and two allele specific primers each comprising at least one single nucleotide polymorphism (SNP) allele-hybridizable sequence, wherein at least one of the allele specific primers also comprises at least one nucleotide alteration. In some embodiments, a nucleic acid is provided comprising a SNP base located within 1-20 bases of its 3′ end. Some embodiments comprise hybridizing the locus specific primer and at least one of the allele specific primers to the nucleic acid, amplifying the hybridized nucleic acid using pyrophosphorolysis activated polymerization (PAP) PCR, and determining the melting temperature (Tm) of the resulting amplicons, for example, using HRM. In some embodiments, reaction mixtures and kits for HRM genotyping are provided. | 11-08-2012 |
20120295255 | METHOD FOR HIGH RESOLUTION MELT GENOTYPING - Various methods are described that provide for high resolution melt (HRM) genotyping. The embodiments include providing a locus specific primer and two allele specific primers each having a 5′ end with a short tail, providing a nucleic acid having a single nucleotide polymorphism (SNP) base located within 1-20 base pairs of the 3′ end of nucleic acid, hybridizing the locus specific primer and the allele specific primers to the nucleic acid, amplifying the sample using pyrophosphorolysis activated polymerization (PAP) PCR enzyme, and determining the Tm of the amplicons using HRM. In other embodiments, reactions mixtures and kits for HRM genotyping are provided and disclosed. These kits comprise a locus specific primer, one or more allele specific primers each having a 5′ end with a short tail, a nucleic acid, and a pyrophosphorolysis activate polymerization (PAP) PCR enzyme. | 11-22-2012 |
Patent application number | Description | Published |
20130062545 | System and Method for Dispensing a Predetermined Amount of a Fluid - A system and method for dispensing a predetermined portion of a beverage or drink additive using a cost effective portion control valve that replaces electrical components with mechanical components. The present invention dispenses a controlled portion of a beverage when a lever is activated. A magnetically coupled linkage system can control the exact amount of fluid dispensed. A valve block contains a beverage input, a beverage outlet, and a valve seal. A lever arm is connected to the valve seal and connected to a magnetic housing containing a valve magnet. A yoke pivots about the valve block where the yoke contains a yoke magnet aligned to interface with the valve magnet. A lever arm return spring is connected to the lever arm and the valve block to bias the lever arm return spring to a resting position. As the yoke pivots beyond a predetermined distance, the yoke magnet separates from the valve magnet allowing the lever arm to return to the resting position closing the valve. | 03-14-2013 |
20130214003 | Beverage Dispenser Using Slurry Ice Heat Transfer - A beverage dispenser that uses a slurry ice heat transfer approach to achieve a lower cost solution for controlling the temperature of a beverage to be dispensed. A cost effective heat transfer approach can be achieved by replacing the cold plate or refrigeration system of prior art beverage dispensers with a slurry ice bath. The invention provides a hopper into which a slurry ice bath is created. The hopper has an access door for receiving ice and an interior valve for dispensing either carbonated water or still water to form a slurry ice bath. Coils containing a beverage to be dispensed are submersed in the slurry ice bath. The slurry ice causes heat to transfer from the coils, thereby maintaining the coils at a certain temperature. | 08-22-2013 |
20130341347 | System and Method for Dispensing a Predetermined Amount of a Fluid - A system and method for dispensing a predetermined portion of a beverage or drink additive using a cost effective portion control valve that replaces electrical components with mechanical components. The present invention dispenses a controlled portion of a beverage when a lever is activated. A magnetically coupled linkage system can control the exact amount of fluid dispensed. A valve block contains a beverage input, a beverage outlet, and a valve seal. A lever arm is connected to the valve seal and connected to a magnetic housing containing a valve magnet. A yoke pivots about the valve block where the yoke contains a yoke magnet aligned to interface with the valve magnet. A lever arm return spring is connected to the lever arm and the valve block to bias the lever arm return spring to a resting position. As the yoke pivots beyond a predetermined distance, the yoke magnet separates from the valve magnet allowing the lever arm to return to the resting position closing the valve. | 12-26-2013 |
Patent application number | Description | Published |
20110049190 | Undercounter ice dispenser - An improved undercounter ice dispenser is disclosed that has a multiple piece auger made in sections for moving ice from the dispenser bin to its ice chute. The auger ice chute invention includes a sensor to reduce ice jamming and to detect when the ice chute is filled or needs filling and works in conjunction with a paddle on the auger to help make the transition. Proportional size ice quantities may also be selected via an interface and auger control. | 03-03-2011 |
20140305316 | Hot/Cold Beverage Dispenser and Method - A beverage dispenser for dispensing either a hot or cold beverage, for example tea, from a common mixing chamber and a method for making and/or operating the same is disclosed. The dispense has a source of hot water, usually a heater, and a source of water, a refrigeration system to cool the water and one or more sources of flavor. The hot or cold water, or both can be provided to the common or same, mixing chamber and mixed assisted with a static and/or dynamic mixer and then dispensed. The last portion of the water is used to rinse for the next beverage. Flavor or syrup pump current is sensed to provide the appropriate amount of flavor concentrate to a known quantity of water to form a beverage of appropriate strength or syrup. | 10-16-2014 |
20140319175 | Multi-Nozzle Beverage Dispenser with Slurry Ice Cooling System - A beverage dispenser that uses a slurry ice bath to achieve heat transfer from coils containing a beverage. The invention provides a hopper into which a slurry ice bath is created. The hopper receives ice from an ice bin via an ice chute. The ice bin contains a rotating agitator for pushing ice down the ice bin. Coils containing a beverage to be dispensed are submersed in the slurry ice bath. Ice passing through the ice chute and into the hopper floats to the surface of the water in the hopper. As the agitator rotates, it continues to push ice down the ice chute, which in turn raises the water level of the water in the hopper. The water level then reaches an equilibrium level, where the buoyant force of the ice in the water will prevent additional ice from falling through the ice chute. | 10-30-2014 |
Patent application number | Description | Published |
20130091181 | MONITORING STORED PROCEDURE EXECUTION - A computer-implemented method, system, and/or computer program product monitors stored procedures that are executed in a database management system. A determination is made, for a stored procedure that is executed in a database management system, of an identification of the stored procedure and event data related to the stored procedure. The event data includes when the stored procedure began and ended executing, an SQL identifier for an SQL statement in the stored procedure, and a counter of how many times the SQL statement executed. The database management system provides values of parameters used when executing the SQL statement. These values are then correlated with the count of how many times the SQL statement executed. | 04-11-2013 |
20130205101 | DYNAMIC REAL STORAGE USAGE CONTROL - Provided are techniques for managing an amount of real storage used by a database management system. A value of a real storage management parameter is received, wherein the real storage management parameter indicates conditions under which one or more virtual storage pages are analyzed to identify one or more unused, virtual storage pages that are to be discarded. The database management system and consumption of real storage and auxiliary storage is monitored. In response to determining that the value of the real storage management parameter is set to on, the one or more unused virtual storage pages are discarded. In response to determining that the value of the real storage management parameter is set to auto and that paging has occurred, the one or more unused, virtual storage pages are discarded. Health values are recorded. | 08-08-2013 |
20150026133 | PRODUCING AN IMAGE COPY OF A DATABASE OBJECT BASED ON INFORMATION WITHIN DATABASE BUFFER POOLS - According to one embodiment of the present invention, a system includes a processor to copy an object of a database system. The system determines one or more portions of the object that are active and reside within a buffer pool of the database system, and copies the determined portions of the object from the buffer pool. Remaining portions of the object that are non-active are copied from database storage. A copy of the object is created from the copied object portions. Embodiments of the present invention further include a method and program product for copying an object of a database system in substantially the same manner described above. | 01-22-2015 |
20150039576 | Managing Transactional Data for High Use Databases - According to one embodiment of the present invention, a system compares database transactions to corresponding performance information to identify conforming database transactions with an acceptable deviation from the performance information and outlier database transactions beyond the acceptable deviation from the performance information. The system aggregates information from a threshold quantity of conforming database transactions into an aggregated record, wherein the threshold quantity is dynamically adjusted, and generates a record for each outlier database transaction, wherein conditions for identifying the outlier database transactions are dynamically adjusted. Embodiments of the present invention further include a method and computer program product for managing database transactions in substantially the same manners described above. | 02-05-2015 |
20150039578 | MANAGING TRANSACTIONAL DATA FOR HIGH USE DATABASES - According to one embodiment of the present invention, a system compares database transactions to corresponding performance information to identify conforming database transactions with an acceptable deviation from the performance information and outlier database transactions beyond the acceptable deviation from the performance information. The system aggregates information from a threshold quantity of conforming database transactions into an aggregated record, wherein the threshold quantity is dynamically adjusted, and generates a record for each outlier database transaction, wherein conditions for identifying the outlier database transactions are dynamically adjusted. Embodiments of the present invention further include a method and computer program product for managing database transactions in substantially the same manners described above. | 02-05-2015 |
20150058855 | MANAGEMENT OF BOTTLENECKS IN DATABASE SYSTEMS - Management is provided for threads of a database system that is subject to a plurality of disparate bottleneck conditions for resources. A monitor thread retrieves, from a first thread, first monitor data for first bottleneck condition of a first type. The monitor thread compares the first monitor data to a trigger level for the first bottleneck condition and then determines, in response to the comparison of the first monitor data to the trigger level, a potential source of the first bottleneck condition. A potential blocker thread is identified based upon the potential source of the first bottleneck condition. The monitor thread retrieves, from the potential blocker thread, second monitor data for a second type of bottleneck condition that is different from the first type of bottleneck condition. Based upon monitor data, a blocking thread is identified, and a particular blocking solution is applied to the blocking thread. | 02-26-2015 |
20150058865 | MANAGEMENT OF BOTTLENECKS IN DATABASE SYSTEMS - Management is provided for threads of a database system that is subject to a plurality of disparate bottleneck conditions for resources. A monitor thread retrieves, from a first thread, first monitor data for first bottleneck condition of a first type. The monitor thread compares the first monitor data to a trigger level for the first bottleneck condition and then determines, in response to the comparison of the first monitor data to the trigger level, a potential source of the first bottleneck condition. A potential blocker thread is identified based upon the potential source of the first bottleneck condition. The monitor thread retrieves, from the potential blocker thread, second monitor data for a second type of bottleneck condition that is different from the first type of bottleneck condition. Based upon monitor data, a blocking thread is identified, and a particular blocking solution is applied to the blocking thread. | 02-26-2015 |
20150081616 | DATABASE INSERT WITH DEFERRED MATERIALIZATION - According to one embodiment of the present invention, a system inserts data into a database object. The system associates the database object with a parameter specifying materialization of data for the database object. The system inserts data into the database object and materializes the data in accordance with the parameter to provide access to the data from the database object, wherein the parameter specifies a portion of the data to be materialized upon insertion. Embodiments of the present invention further include a method and computer program product for inserting data into a database object in substantially the same manners described above. | 03-19-2015 |
20150081745 | DATABASE INSERT WITH DEFERRED MATERIALIZATION - According to one embodiment of the present invention, a system inserts data into a database object. The system associates the database object with a parameter specifying materialization of data for the database object. The system inserts data into the database object and materializes the data in accordance with the parameter to provide access to the data from the database object, wherein the parameter specifies a portion of the data to be materialized upon insertion. Embodiments of the present invention further include a method and computer program product for inserting data into a database object in substantially the same manners described above. | 03-19-2015 |