Patent application number | Description | Published |
20100022414 | Droplet Libraries - The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays. | 01-28-2010 |
20100137163 | Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors - The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds. | 06-03-2010 |
20130090248 | SELECTION OF COMPARTMENTALISED SCREENING - The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids. | 04-11-2013 |
20130183659 | MICROFLUIDIC DEVICES - The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids. | 07-18-2013 |
20130210639 | MICROFLUIDIC DEVICES - The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids. | 08-15-2013 |
20130217583 | MICROFLUIDIC DEVICES AND METHODS OF USE IN THE FORMATION AND CONTROL OF NANOREACTORS - The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds. | 08-22-2013 |
20140256595 | MICROFLUIDIC DEVICES AND METHODS OF USE IN THE FORMATION AND CONTROL OF NANOREACTORS - The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library. | 09-11-2014 |
20140323317 | MICROFLUIDIC DEVICES AND METHODS OF USE IN THE FORMATION AND CONTROL OF NANOREACTORS - The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library. | 10-30-2014 |
Patent application number | Description | Published |
20090011959 | Method for preparing single-stranded DNA libraries - This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support. | 01-08-2009 |
20090048124 | Methods of amplifying and sequencing nucleic acids - An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step. | 02-19-2009 |
20100261177 | INCREASING SPECIFICITY IN A scFV SCREEN USING DUAL BAIT REPORTERS - To increase the efficiency of the selection of antibodies of desired specificity, we create multi-bait strain(s) in which one bait is the target and one or more bait(s) are non-target. The non-target bait(s) may use one or more DNA-binding domain(s) that differ(s) from that of the target bait and thereby activate one or more different reporters from that activated by the target bait. Library hits that activate both sets of reporters are presumed to be inadequately specific and can be eliminated from further consideration. Alternatively, a non-target bait may be replaced with a second target bait, and hits selected that activate both sets of reporters. Other combinations of elements can be used. | 10-14-2010 |
20100305004 | POLYNUCLEOTIDE BACKBONES FOR COMPLEXING PROTEINS - We use the Tus-Ter interaction to enable the utilization of nucleic acid analytical methodologies for proteins. We also use the Tus-Ter interaction to make polymers and oligomers that have a nucleic acid backbone with protein functionalities. These methods are useful for molecular modeling, for efficiently running enzymatic pathway reactions, and for analyzing presence and/or amount of particular proteins. | 12-02-2010 |
20110009275 | Methods of amplifying and sequencing nucleic acids - An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step. | 01-13-2011 |
20110143955 | Protein Capture, Detection and Quantitation - This invention is related to the area of the capture, detection and quantitation of proteins. In particular, it relates to making and using recombinant antibodies bound to oligonucleotides and using such complexes for analytic purposes. These techniques are designed to permit multiplexed detection and quantitation of very large numbers of proteins. | 06-16-2011 |
20110166027 | INTERACTION SCREENING METHODS, SYSTEMS AND DEVICES - An interaction screening method for identifying binding moieties encapsulates candidate binding moieties in droplets with a first known moiety and a second known moiety. The candidate binding moieties are different in the different droplets. The method further comprises determining for one or more of the droplets whether the candidate binding moiety is bound to the first known moiety and/or the second known moiety. Optionally, the method further comprises segregating at least one droplet in which the candidate binding moiety is bound to the first known moiety or to the first and second known moiety. | 07-07-2011 |
20110201526 | BEAD EMULSION NUCLEIC ACID AMPLIFICATION - Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention. | 08-18-2011 |
20110267457 | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING - The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area. | 11-03-2011 |
20120238475 | Methods of Amplifying and Sequencing Nucleic Acids - An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step. | 09-20-2012 |
20130059741 | BINDING ASSAYS FOR MARKERS - This invention provides compositions and methods for assaying the presence of a target analyte in a sample using a solid support. Embodiments of the present invention provide a solid support having a binding protein, such as an antibody, antibody fragment or protein receptor, immobilized to the solid support and at least two separate nucleic acid primers immobilized near the binding protein. This invention also provides a method for tethering two or more polypeptide subunits to generate a multifunctional fusion protein, which can have a primary function, e.g., binding a target analyte, such as a target protein, or an enzymatic activity, and one or more of the subunits of the fusion protein carries out a secondary function, e.g., capture on a solid matrix or quantitation. | 03-07-2013 |
20130078638 | Bead Emulsion Nucleic Acid Amplification - Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention. | 03-28-2013 |
20140162885 | Bead Emulsion Nucleic Acid Amplification - Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention. | 06-12-2014 |
20140342921 | EXPANDED RADIX FOR POLYMERIC TAGS - A method having steps of (a) providing nucleic acids having a tag sequence (N | 11-20-2014 |
20150099672 | BEAD EMULSION NUCLEIC ACID AMPLIFICATION - Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention. | 04-09-2015 |
Patent application number | Description | Published |
20100222993 | System and method for improved rotary-wing aircraft performance with interior/external loads - An interactive aircraft load management system automates calculation and provides simulation capability to changes in an aircraft C.G. limit for display on a three-dimensional aircraft symbology. The aircraft load management system also communicates with a fly by wire (FBW) flight control system wherein the aircraft's control system is programmed to automatically compensate for C.G. excursions and to alter control laws. The aircraft load management system also selectively reels-in and reels out sling lines as the aircraft pitches and rolls to maintain a load vector from a slung load along the aircraft centerline. Load vector travel is accomplished by coupling a winch control system into the flight control system. | 09-02-2010 |
20130228646 | RETRACTING TAILWHEEL ASSEMBLY AND FIN - A tailwheel assembly for an aircraft includes a ventral fin including a fixed airframe portion of the ventral fin and a moveable tailwheel portion of the ventral fin. A tailwheel strut extends through the ventral fin and includes a fixed first strut portion and an extendable second strut portion having the moveable tailwheel portion of the ventral fin secured thereto. A tailwheel is secured to the extendable second strut portion. A method of operating a tailwheel assembly for an aircraft includes extending an extendable portion of a tailwheel strut from an airframe of the aircraft. The tailwheel strut has a tailwheel secured thereto and is located at least partially in a ventral fin of the aircraft. A tailwheel portion of the ventral fin is moved away from a fixed airframe portion of the ventral fin via extension of the extendable portion of the tailwheel strut. | 09-05-2013 |
20140215999 | AFT EXHAUST SYSTEM FOR ROTARY WING AIRCRAFT - An exhaust system for reducing infrared emissions of a rotary wing aircraft includes a duct assembly having an inlet portion and an outlet portion; the inlet portion configured to receive exhaust from an engine of the aircraft; and the outlet portion coupled to the inlet portion, the outlet portion having an outlet duct with an outlet opening, the outlet duct configured expel an emission containing engine exhaust proximate to a tail fairing of the rotary wing aircraft. | 08-07-2014 |
20140314563 | VIBRATION CONTROL OF A SWASHPLATELESS COAXIAL ROTOR - A rotary wing aircraft is provided including a dual counter-rotating, coaxial rotor system having an upper rotor system and a lower rotor system rotatable about a common axis. A plurality of blade assemblies is mounted to a portion of either the upper rotor system or the lower rotor system. A plurality of individually controllable actuators is coupled to each of the plurality of blade assemblies. Each of the plurality of actuators is configured to control movement of the coupled blade assembly about a pitch axis. The rotary-wing aircraft additionally includes a sensor system within an airframe. A higher harmonic control (HHC) controller is arranged in communication with the sensor system and the plurality of actuators to individually control the upper rotor system and the lower rotor system to reduce vibration. | 10-23-2014 |
20150028597 | SWASHPLATELESS COAXIAL ROTARY WING AIRCRAFT - A main rotor system of an aircraft is provided including a first rotor coupled to a transmission and configured to rotate about an axis in a first direction. A second rotor is similarly coupled to the transmission and is configured to rotate about the axis in a second direction. At least the first rotor includes an individual blade control system (IBCS) configured to adjust a pitch of each of a plurality of blade of the first rotor independently. At least one slip ring is configured to transmit electrical power and/or a control signal to the at least one IBCS. | 01-29-2015 |
20150097075 | YAW CONTROL OF CO-AXIAL ROTOR - A method for controlling rotor blades of a co-axial rotor assembly of an aircraft including a first rotor co-axial with a second rotor includes identifying a first zone of rotor rotation angles of the co-axial rotor assembly. The first zone defines a range of rotor rotation angles corresponding to an up-flow of air to the coaxial rotor assembly, and the remainder of the rotor rotation angles other than the first zone of rotation angles is defined as a second zone. The method includes receiving a yaw command to adjust a yaw moment of the aircraft and applying a different rotor blade angle change to rotor blades in the first zone than a rotor blade angle change applied to rotor blades in the second zone to adjust the yaw moment of the aircraft according to the yaw command. | 04-09-2015 |
20150314866 | ACCELERATION SMOOTHING HOLDING OVERALL KINETIC ENERGY CONTROL - A method and system for controlling maneuverability of an aircraft includes receiving one or more signals indicative of commanded peak rotary acceleration at a first timeperiod; determining a signal indicative of an actual peak rotary acceleration for the first timeperiod in response to the receiving of the one or more signals for commanded pilot acceleration; and determining signals indicative of actual rotary acceleration for a second timeperiod. | 11-05-2015 |