43rd week of 2021 patent applcation highlights part 26 |
Patent application number | Title | Published |
20210332326 | METHODS FOR CULTURING CELLS - The preset disclosure provides methods of culturing cells, e.g., pluripotent cells, multipotent cells, and/or immune cells (e.g., T cells, NK cells, and/or TILs) in a medium comprising at least about 5 mM potassium ion, wherein the medium is not hypertonic. In some aspects, the medium is hypotonic. In some aspects, the methods disclosed herein increases the number of less-differentiated cells in the population of cells. In some aspects, the cultured cells are engineered, e.g., to comprise a chimeric antigen receptor or an engineered T cell receptor. In some aspects, the cells are administered to a subject in need thereof. | 2021-10-28 |
20210332327 | AUTOLOGOUS SOMATIC STEM CELL THERAPY, METHOD OF CONTROLLABLE PREPARATION OF THERAPEUTIC COMPOSITION AND PROCEDURE OF ADAPTIVE TREATMENT OF IVF PATIENT - A blood product containing peripheral blood mononuclear cells (PBMCs) in an amount of at least 4 million cells per milliliter and human chorionic gonadotropin (HCG in an amount of at least 150 international units (IU) per milliliter. A method of preparing the blood product, including applying HCG to a female patient, then obtaining PBMCs from the female patient, then adding HCG to the obtained PBMCs. A method of culturing PBMCs, including applying HCG to a female patient, then culturing PBMCs obtained from the female patient at a time after the HCG was applied to the patient. A method of in vitro fertilization, including applying HCG to a female patient, culturing PBMCs obtained from the patient after the HCG was applied to the patient, introducing the cultured PBMCs into the uterus of the patient, and transferring at least one embryo into the uterus of the patient. | 2021-10-28 |
20210332328 | Human Vgamma9Vdelta2T Cell Proliferation Culture Method and Culture Medium - A human Vγ9Vδ2T cell proliferation method and a culture medium. The method comprises: first stimulating a human Vγ9Vδ2T cell with a culture medium containing interleukin-2 and phosphonic acid compounds, and then culturing the human Vγ9Vδ2T cell with a culture medium added with interleukin-15 and vitamin C to achieve proliferation culture. The culture medium additionally comprises interleukin-15 and vitamin C. Compared with a conventional proliferation method and a conventional culture medium, the method and the culture medium can improve the proliferation efficiency and cell purity of the human Vγ9Vδ2T cell. The human Vγ9Vδ2T cell obtained by culture in the method has stronger anti-apoptotic ability and longer cell survival time, and moreover, the expression level of critical killer molecules NKG2D thereof is higher, thereby having stronger killing ability to tumor cells. | 2021-10-28 |
20210332329 | NOVEL RENAL PROGENITOR CELL MARKER AND METHOD FOR CONCENTRATING RENAL PROGENITOR CELLS USING SAME - A method for concentrating renal progenitor cells involves extracting MET-positive cells and/or AGTR2-positive cells from a renal progenitor cell-containing cell population. The MET-positive cells and/or AGTR2-positive cells are extracted using an anti-MET antibody and/or an anti-AGTR2 antibody. The renal progenitor cell-containing cell population is obtained from pluripotent stem cells. The renal progenitor cells are OSR1 (odd-skipped related 1)-positive and SIX2 (SIX Homeobox 2)-positive. | 2021-10-28 |
20210332330 | AUTOMATED SYSTEM FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS OR DIFFERENTIATED CELLS - The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells. | 2021-10-28 |
20210332331 | Adeno-Associated-Virus Rep Sequences, Vectors and Viruses - The invention provides adeno-associated virus (AAV) replication (Rep) sequences. In one embodiment, the invention provides nucleotide sequences encoding a chimeric protein, wherein the encoded chimeric protein contains a wild type AAV Rep inhibitory amino acid sequence, and wherein the nucleotide sequences contain a scrambled and/or deoptimized polynucleotide sequence encoding the wild type AAV Rep inhibitory amino acid sequence. The invention provides vectors, cells, and viruses containing the invention's sequences. Also provided are methods for detecting portions of the AAV Rep inhibitory amino acid sequence, which reduce replication and/or infection and/or productive infection by viruses. The invention's compositions and methods are useful for site-specific integration and/or expression of heterologous sequences by recombinant adeno-associated virus (rAAV) vectors and by rAAV virus particles, such as hybrid viruses (e.g., Ad-AAV) comprising such vectors. The invention's compositions and methods find application in, for example, gene therapy and/or vaccines. | 2021-10-28 |
20210332332 | MUTANT MICROORGANISM INTRODUCED WITH HIGHLY ACTIVE MALATE DEHYDROGENASE FOR PRODUCING SUCCINIC ACID AND METHOD OF PRODUCING SUCCINIC ACID USING THE SAME - Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology. | 2021-10-28 |
20210332333 | METHODS AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS - The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC. | 2021-10-28 |
20210332334 | CHIMERIC ANTIGEN RECEPTOR POLYPEPTIDES IN COMBINATION WITH TRANS METABOLISM MOLECULES MODULATING KREBS CYCLE AND THERAPEUTIC USES THEREOF - Disclosed herein are genetically engineered hematopoietic cells, which express one or more Krebs cycle modulating polypeptides, and optionally a chimeric receptor polypeptide (e.g., an antibody-coupled T cell receptor (ACTR) polypeptide or a chimeric antigen receptor (CAR) polypeptide) capable of binding to a target antigen of interest. Also disclosed herein are uses of the engineered hematopoietic cells for inhibiting cells expressing a target antigen in a subject in need thereof. | 2021-10-28 |
20210332335 | IN VIVO PROTEIN N-ACYLATION - Described are a genetically modified microorganism and corresponding methods and products. The genetically modified microorganism may include a first gene that encodes an acyl transferase and a second gene that encodes a peptide or protein. One or both of the first and second gene may be heterologous. The genetically modified microorganism may include a modified acyl-CoA biosynthetic pathway configured for one or more of: inducible biosynthesis of an acyl-CoA and over-accumulation of the acyl-CoA. The genetically modified microorganism may be effective upon fermentation to cause acylation of the peptide or protein by the acyl transferase using the acyl-CoA to provide a N-acylated peptide or protein product. | 2021-10-28 |
20210332336 | CALCIUM DEPENDENT PROTEIN KINASE CONSTRUCTS AND USES THEREOF - The present invention relates to calcium dependent protein kinase (CORK) constructs comprising a pair of chromophores suitable for measuring real-time FRET occurrence upon conformational changes or activation of the CORK construct. Particularly, the present invention relates to CORK polypeptides comprising a variable domain (VD), a protein kinase domain (PKD), a pseudosubstrate segment (PS), and a calmodulin-like domain (OLD), said CLD comprising 4 EF-hand motifs EF1, EF2, EF3 and EF4, wherein a donor chromophore is inserted between PKD and PS and an acceptor chromophore is inserted C-terminally of EF4, or an acceptor chromophore is inserted between PKD and PS and a donor chromophore is inserted C-terminally of EF4, wherein said donor chromophore and said acceptor chromophore represent a Förster Resonance Energy Transfer (FRET) pair. The present invention further relates to polynucleotides encoding such polypeptides as well as vectors and host cells comprising such polypeptides and/or polynucleotides. Furthermore, the present invention relates to methods for measuring conformational change or activation status of a CDPK polypeptide by employing said polypeptides and to uses of said polypeptides for measuring conformational change or activation status of a CDPK polypeptide. | 2021-10-28 |
20210332337 | ALKALINE PHOSPHATASE COMPOSITION, METHOD OF PRODUCING DEPHOSPHORYLATED NUCLEIC ACID AND METHOD OF PRODUCING LABELED NUCLEIC ACID - A composition contains an alkaline phosphatase; and a peptide fragment group (A) composed of two or more peptide fragments, wherein each of the two or more peptide fragments consists of 5 to 50 consecutive amino acid residues selected from positions 501 to 578 of the amino acid sequence set forth in SEQ ID NO: 5, wherein a content ratio of the peptide fragment group (A) to the alkaline phosphatase satisfies formula (A): (X | 2021-10-28 |
20210332338 | FUSION MOLECULES OF RATIONALLY-DESIGNED DNA-BINDING PROTEINS AND EFFECTOR DOMAINS - Targeted transcriptional effectors (transcription activators and transcription repressors) derived from meganucleases are described. Also described are nucleic acids encoding same, and methods of using same to regulate gene expression. The targeted transcriptional effectors can comprise (i) a meganuclease DNA-binding domain lacking endonuclease cleavage activity that binds to a target recognition site; and (ii) a transcription effector domain. | 2021-10-28 |
20210332339 | NOVEL NUCLEASE DOMAIN AND USE THEREOF - The present invention provides an artificial nucleic acid-cleaving enzyme comprising: a nuclease domain which is a polypeptide containing an amino acid sequence set forth at positions 391 to 585 of SEQ ID NO: 1 or positions 389 to 579 of SEQ ID NO: 3, or a mutant polypeptide thereof; and a nucleic acid-binding domain. | 2021-10-28 |
20210332340 | COMPOSITIONS COMPRISING POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYPEPTIDES HAVING ARABINOFURANOSIDASE ACTIVITY - The present invention relates to compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity for use in, e.g., animal feed. The present invention further relates to polypeptides having arabinofuranosidase activity, polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2021-10-28 |
20210332341 | CODON OPTIMIZED GLA GENES AND USES THEREOF - The present disclosure provides codon optimized nucleotide sequences encoding human alpha-galactosidase A, vectors, and host cells comprising codon optimized alpha-galactosidase A sequences, and methods of treating disorders such as Fabry disease comprising administering to the subject a codon optimized sequence encoding human alpha-galactosidase A. | 2021-10-28 |
20210332342 | ENZYMES FOR TRIMMING OF GLYCOPROTEINS - The invention concerns fusion proteins, wherein two endoglycosidases are fused, possibly via a linker. The fusion enzymes according to the invention have structure (1): EndoX-(L) | 2021-10-28 |
20210332343 | Polypeptides Having Protease Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having protease activity, animal feed additives comprising said polypeptides improving the nutritional value of an animal feed by adding the protease, invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. | 2021-10-28 |
20210332344 | DIRECTED MODIFICATION OF RNA - Described herein are compositions, systems, methods, and kits utilizing CRISPR-Cas protein fusions comprising a guide nucleotide sequence-programmable RNA binding protein and a RNA base modification protein. The compositions, systems, methods, and kits described herein are useful to modulate RNA methylation and/or cytidine deamination. | 2021-10-28 |
20210332345 | Methods for Terpenoid Production - A bacterial strain comprising one or more vectors encoding a) one or more enzymes to produce one or more terpene precursors; and b) a fungal terpene synthase (FTPS). The present invention also relates to a method of producing a terpenoid comprising a) culturing the bacterial strain described herein in an expression medium; and b) isolating the terpenoid from said expression medium. | 2021-10-28 |
20210332346 | THERMORESPONSIVE SOLUBILITY OF PROTEINS IN AQUEOUS AND NON-NATIVE SOLVENTS BY TUNING MOLECULAR INTERACTIONS VIA POLYMER MODIFICATION - The invention provides methods and systems by which enzymes can be modified to improve solubility, catalytic activity, recoverability, and recyclability. The enzyme may be modified with a thermosensitive copolymer to form an enzyme-polymer conjugate that exhibits upper critical solution temperature (UCST) and/or lower critical solution temperature (LCST)-type behavior in an organic solvent, ionic liquid, or other solvent. Methods and systems of the invention facilitate the use of enzymes as biocatalysts in solvents. | 2021-10-28 |
20210332347 | METHODS FOR TARGETED INSERTION OF DNA IN GENES - Methods and compositions for modifying the coding sequence of endogenous genes using rare-cutting endonucleases and transposases. The methods and compositions described herein can be used to modify the coding sequence of endogenous genes. | 2021-10-28 |
20210332348 | NUCLEIC ACID EXTRACTION COMPOSITION, REAGENT AND KIT CONTAINING THE SAME AND USE THEREOF - A nucleic acid extraction composition, reagents and kits containing the same and uses thereof. Provided is a nucleic acid extraction and purification reagent free of volatile organic solvents, which prevents the damage of volatile organic solvents to people and greatly improves the timeliness of nucleic acid extraction and purification, making the operation extremely simple, and the nucleic acid can be obtained within 10 minutes. The obtained nucleic acid may be used for biological reactions such as PCR, NASBA, LAMP and RPA. Moreover, the reagents of the present disclosure may be used to extract nucleic acids of cells, bacteria, fungi, DNA viruses and RNA viruses from various samples such as blood, throat swab preserving fluid, saliva, urine, sputum, excrement and the like, and very suitable for clinical and scientific research uses. | 2021-10-28 |
20210332349 | MODULATING POLYMER BEADS FOR DNA PROCESSING - Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest. | 2021-10-28 |
20210332350 | Recombinase Genome Editing - A method of altering a target nucleic acid sequence within a cell is provided including providing the cell with a donor nucleic acid, providing the cell with a single strand annealing protein, and providing the cell with a single strand DNA binding protein, wherein one or more or both of the single strand annealing protein and the single strand DNA binding protein is foreign to the cell, and wherein the donor nucleic acid is recombined into the target nucleic acid. | 2021-10-28 |
20210332351 | Massively Parallel Enzymatic Synthesis of Nucleic Acid Strands - The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups. | 2021-10-28 |
20210332352 | COMPOSITIONS, METHODS, MODULES AND INSTRUMENTS FOR AUTOMATED NUCLEIC ACID-GUIDED NUCLEASE EDITING IN MAMMALIAN CELLS VIA VIRAL DELIVERY - This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells. | 2021-10-28 |
20210332353 | ARGONAUTE PROTEIN MUTANT AND USE THEREOF - The present invention relates to a mutant of Argonaute protein lacking a DNA cleavage activity but having a DNA binding activity, wherein the mutation of the mutant is located in a PIWI domain. The present invention also relates to a use based on the protein mutant, especially in enrichment of a target DNA and construction of sequence libraries. Therefore, the present invention also relates to a method for enrichment of a target DNA, comprising the following steps: (a) designing a guide sequence for a specific sequence in the target DNA; (b) binding the mutant according to the present invention, the guide sequence and the target DNA to obtain a mutant-guide sequence-target DNA ternary complex; (c) capturing the mutant-guide sequence-target DNA ternary complex through a capture medium; and (d) separating the target DNA from the captured mutant-guide sequence-target DNA ternary complex to obtain an enriched target DNA. | 2021-10-28 |
20210332354 | SYSTEMS AND METHODS FOR IDENTIFYING DIFFERENTIAL ACCESSIBILITY OF GENE REGULATORY ELEMENTS AT SINGLE CELL RESOLUTION - A method for ascertaining differential accessibility of (TF) binding motifs in open chromatin regions of cells, comprising receiving a cell barcode genomic sequence dataset; aligning each of a plurality of fragment sequence reads to a reference sequence; identifying peaks defined by the aligned plurality of fragment sequence reads; generating a peak-barcode matrix that is comprised of peaks for each cell barcode; clustering cells with peaks having similar chromatin accessibility profiles into a cell cluster to form one or more cell clusters; generating a TF barcode matrix that maps each peak in the peak-barcode matrix to one or more given TF binding motif(s); performing a differential accessibility analysis, wherein the analysis identifies differences in accessibility of peaks and TF binding motifs associated with each identified cell cluster relative to all other identified cell clusters; and generating an output of one or more refined cell clusters based on the differential accessibility analysis. | 2021-10-28 |
20210332355 | STRAND DISPLACEMENT STOP (SDS) LIGATION - A method of ligating DNA molecules, wherein the DNA molecules are in a hybrid with an RNA molecule, including the steps of providing DNA molecules that are in a RNA:DNA hybrid with an RNA molecule, and ligating the DNA molecules to each other with a double strand specific ligase | 2021-10-28 |
20210332356 | NUCLEIC ACID CONSTRUCTS COMPRISING GENE EDITING MULTI-SITES AND USES THEREOF - Disclosed herein is a polynucleotide construct comprising one or more nuclease recognition sequences upstream and downstream of a Gene editing multi-site that comprises a plurality of nuclease recognition sequences. The plurality of nuclease recognition sequences facilitate insertion of one or more exogenous donor genes into the host cell. | 2021-10-28 |
20210332357 | Protecting RNAs from Degradation Using Engineered Viral RNAs - This invention is in the field of molecular biology, gene expression, functional genomics, and bioinformatics and relates to novel RNA and related structures and methods of use thereof that enables modulation of gene expression and preservation of particular transcriptome targets. The invention contemplates various applications of RNA sequences derived from the genomic RNA of flaviviruses (FVs) and the application of such features in combination with heterologous sequences. | 2021-10-28 |
20210332358 | MINIATURIZED HAIRPIN RNAi TRIGGERS (mxRNA) AND METHODS OF USES THEREOF - The present invention relates to novel RNAi triggers that can be chemically synthesized and used to modulate gene expression inside animal cells to study various genes function in laboratories or as an active ingredient for agricultural, veterinary, cosmetic and/or therapeutic applications | 2021-10-28 |
20210332359 | COMPOSITIONS AND METHODS FOR IMMUNE CHECKPOINT INHIBITION - Therapeutic compositions and methods for treating cancer, e.g., pancreatic cancer, that use nanoparticles linked to inhibitory nucleic acids, e.g., siRNAs, targeting an immune checkpoint molecule, e.g., programmed cell death 1 ligand 1 (PD-L1). | 2021-10-28 |
20210332360 | COMPOSITIONS AND METHODS INVOLVING NON-NATURAL APTAMER LIBRARIES - The disclosure is directed to methods and compositions for screening a library of aptamers for aptamers having a binding affinity to a target molecule. Specifically, non-natural nucleotides can be introduced onto aptamers immobilized on the surface of beads. The non-natural nucleotides can then be subsequently chemically modified to include additional binding agents. For example, copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions can be used to introduce a wide range of binding agents onto non-natural nucleotides on beads. | 2021-10-28 |
20210332361 | APTAMER THERAPEUTICS USEFUL IN THE TREATMENT OF COMPLEMENT-RELATED DISORDERS - The invention provides nucleic acid therapeutics and methods for using these nucleic acid therapeutics in the treatment of complement-related disorders. | 2021-10-28 |
20210332362 | Aptamers Against Clostridium Difficile - Compositions comprising aptamers capable of specifically binding to a surface protein of | 2021-10-28 |
20210332363 | BLOOD-BRAIN BARRIER PERMEABLE APTAMER AND APPLICATION THEREOF - The present invention relates to an aptamer that penetrating the blood-brain barrier (BBB), and relates to an application of such an aptamer to a complex combined with a substance that selectively binds to a target substance present in the brain. The aptamer and the complex combined with the aptamer can be used as a positron emission tomography (PET) radiographic contrast agent to diagnose Alzheimer's disease or Parkinson's disease at an early stage. It can be used as a radiographic material that can be imaged on, and can be used as a treatment for degenerative brain diseases. | 2021-10-28 |
20210332364 | siNA MOLECULES, METHODS OF PRODUCTION AND USES THEREOF - The present disclosure relates to method of producing and using short interfering nucleic acids (siNAs) for preventing and treating coronavirus-inflicted infectious conditions. In particular, this disclosure relates to the method of producing and using siNAs for preventing and treating infections by the coronavirus SARS-CoV-2, the causative viral agent of the novel coronavirus disease COVID-19, to mediate gene silencing of viral proteins. The present disclosure is also directed to interfering RNA duplexes and vectors encoding such interfering RNA duplexes. | 2021-10-28 |
20210332365 | HEPATITIS B VIRUS (HBV) dsRNA AGENT COMPOSITIONS AND METHODS OF USE THEREOF - The present disclosure relates to double stranded RNA agents targeting the hepatitis B virus (HBV) genome, and methods of using such agents to inhibit expression of one or more HBV genes and methods of treating subjects having an HBV infection or HBV-associated disorder, e.g., chronic hepatitis B infection. | 2021-10-28 |
20210332366 | Novel Small Activating RNA - saRNAs are provided in the present invention. The saRNAs are composed of a first oligonucleotide strand containing 17 to 30 nucleotides and a second oligonucleotide strand containing 17 to 30 nucleotides. Sequences of at least 15 nucleotides in length are complementary in the two oligonucleotide strands, and the unpaired terminal nucleotides form overhangs. The first oligonucleotide strand or the second oligonucleotide strand has more than 75% homology or complementarity with any continuous fragment of 16 to 35 nucleotides in length in the promoter of the target gene. The second oligonucleotide strand has an overhang composed of 1 to 4 nucleotides at 3′ end. saRNAs of the present invention can upregulate target gene expression more effectively while reducing off-target effects. | 2021-10-28 |
20210332367 | KETOHEXOKINASE (KHK) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The present invention relates to RNAi agents, e.g., dsRNA agents, targeting the ketohexokinase (KHK) gene. The invention also relates to methods of using such RNAi agents to inhibit expression of a KHK gene and to methods of treating or preventing a KHK-associated disease in a subject. | 2021-10-28 |
20210332368 | COMPOSITIONS AND METHODS TO RESTORE PATERNAL UBE3A GENE EXPRESSION IN HUMAN ANGELMAN SYNDROME - The present invention relates to compositions and methods for activating expression from the paternally-inherited allele of UBE3A in human Angelman's Syndrome neurons using viral vector delivery of short hairpin RNAs, ribozymes, and/or microRNAs. | 2021-10-28 |
20210332369 | USE OF CBX4 AS TARGET FOR ACTIVATION OF HIV-1 LATENT INFECTION - The present invention relates to a use of CBX4 as a target for activation of HIV-1 latent infection. The present invention found by research that, inhibiting CBX4 causes a good ability for activation of HIV-1 latent, a knockdown of CBX4 can effectively promote a transcription of LTR of HIV-1; and afterwards the present invention has found that, by the knockdown of CBX4 in a J-lat 10.6 cell model of HIV-1 latent infection, GFP gene expression is up-regulated, and HIV-1 can be effectively activated. An overexpression of the CBX4 protein in TZM-bl cells can effectively decrease a transcriptional activity of LTR of HIV-1. It is found by further detection that, decreased expression of CBX4 protein will reduce a degree of enrichment of H3K9 trimethylation and H3K27 trimethylation of LTR of HIV-1, thereby activating HIV-1 latent infection. | 2021-10-28 |
20210332370 | METHODS FOR PRODUCING CARBO SUGARS AND APPLICATIONS THEREOF - Described herein are biological devices and methods for using the same to produce carbo sugars. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a cellulose synthase and galactomannan galactosyltransferase. In some instances, the biological devices also include a gene for lipase. Methods for altering the viscosity of petroleum oil using the carbo sugars are also described herein. Finally, methods for degreasing or decontaminating water mixed with petroleum oil or other fatty substances or a surface coated with petroleum oil or other fatty substances using the carbo sugars are described herein. | 2021-10-28 |
20210332371 | NUCLEIC ACID TO ACTIVATE GENE EXPRESSION AND PROTEIN PRODUCTION - A method for increasing the efficiency of protein production is described, said method employing a a nucleic acid with a specific non-coding nucleotide sequence, which strongly increases protein production in several eukaryotic systems, as well as the compositions that contain this nucleic acid sequence. | 2021-10-28 |
20210332372 | METHOD FOR IMPROVING BIO-COUPLING EFFICIENCY BETWEEN PROTEIN AND NUCLEIC ACID BASED ON ALPHA-HELIX HANDLE - A method for improving a bio-coupling efficiency between a protein and a nucleic acid based on an α-helix handle includes the following steps. First, the handle carrying the non-natural amino acid (H-tag) is designed. Then, a recombinant expression plasmid encoding a fusion protein containing the H-tag and the protein to be tested is constructed. Subsequently, the fusion recombinant protein containing the non-natural amino acid in the H-tag is expressed and purified. Finally, the non-natural amino acid in the H-tag-fused protein and the coupling group on the nucleic acid substrate are efficiently connected by click chemistry. Thea-helix handle is used to provide a controllable reaction condition on the protein surface for the non-natural amino acid, avoiding the complex structure, charge and polar nanoenvironment around the surface of the protein to be tested. | 2021-10-28 |
20210332373 | E. COLI-BASED PRODUCTION OF BETA-LACTAMASE - The invention relates to, in part, improved methods for the production of beta-lactamase using | 2021-10-28 |
20210332374 | MICROORGANISMS AND METHODS FOR PRODUCING CANNABINOIDS AND CANNABINOID DERIVATIVES - The present disclosure provides genetically modified host cells that produce a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative. The present disclosure provides methods of synthesizing a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative. | 2021-10-28 |
20210332375 | METHODS FOR IMPROVING COMPETENCY OF PLANT CELLS - The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture. | 2021-10-28 |
20210332376 | Transcriptional Regulation for Improved Plant Productivity - Methods comprising DNA constructs and polynucleotides of functional transcription factors for improving photosynthetic capacity, biomass and/or grain yield and stress tolerance in various crop and model plants, dicots and monocots with the C | 2021-10-28 |
20210332377 | GENETICALLY ENGINEERED PLANTS THAT EXPRESS A QUINONE-UTILIZING MALATE DEHYDROGENASE - Genetically engineered plants that express a quinone-utilizing malate dehydrogenase (MQO) are provided. The plant comprises a modified gene for the quinone-utilizing malate dehydrogenase. The modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the quinone-utilizing malate dehydrogenase. The promoter is non-cognate with respect to the nucleic acid sequence encoding the quinone-utilizing malate dehydrogenase. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the quinone-utilizing malate dehydrogenase. The plants can express the quinone-utilizing malate dehydrogenase in mitochondria of cells of the plants. Conversion of malate to oxaloacetate in the mitochondria can be increased, resulting in increased crop performance and/or seed, fruit or tuber yield. Methods and compositions for making the plants also are provided. | 2021-10-28 |
20210332378 | PLANT REGULATORY ELEMENTS AND USES THEREOF - The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use. | 2021-10-28 |
20210332379 | IDENTIFICATION OF RESISTANCE GENES FROM WILD RELATIVES OF BANANA AND THEIR USES IN CONTROLLING PANAMA DISEASE - The present disclosure provides compositions and methods for providing broad-based resistance to fungal pathogens, such as a | 2021-10-28 |
20210332380 | Transgenic Corn Event MON95275 and Methods for Detection and Uses Thereof - The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275. | 2021-10-28 |
20210332381 | Ligand Matched Transcription Control, Control Devices, and Solute Carriers - In an aspect, the present invention relates generally to RNA control devices, destabilizing elements (“DE”), Solute Carrier Transporters (“SLC”), and/or control regions where some or all of these control elements are ligand matched. In another aspect, the present invention relates to ligand matched control regions, SLCs and/or control devices that produce control systems with desired properties. In a further aspect, the present invention relates to use of these control systems to control expression of transgenes in eukaryotic cells including, for example, stem cells, hematopoietic cells, and host cells for gene therapy. | 2021-10-28 |
20210332382 | CANCER THERAPY - The invention concerns a modified oncolytic adenovirus; a pharmaceutical composition comprising same; and a method of treating cancer using same. | 2021-10-28 |
20210332383 | Adeno Associated Virus Vectors for the Treatment of Hunter Disease - The present disclosure provides, among other things, a recombinant adeno-associated virus (rAAV) vector comprising an AAV8 or AAV9 capsid and a codon-optimized sequence encoding a human iduronate-2-sulfatase (I2S) enzyme. The disclosure also provides a method of treating a subject having Hunter syndrome (MPS II), comprising administering to the subject in need thereof a recombinant adeno-associated virus (rAAV) vector comprising an AAV8 or AAV9 capsid, and a promoter operably linked to a nucleic acid sequence that encodes iduronate-2-sulfatase (I2S), and wherein administering results in an increase in I2S enzymatic activity in the subject. | 2021-10-28 |
20210332384 | Oncolytic Vaccinia Virus With Modified B5R Gene For The Treatment Of Cancer - The present invention relates to a vaccinia virus vector comprising a nucleic acid sequence encoding a SCR1-, SCR2-, SCR3-, and SCR4-domain deleted B5R gene (B5R SCR1 | 2021-10-28 |
20210332385 | GENE THERAPIES FOR LYSOSOMAL DISORDERS - The disclosure relates, in some aspects, to compositions and methods for treatment of diseases associated with aberrant lysosomal function, for example Parkinson's disease and Gaucher disease. In some embodiments, the disclosure provides expression constructs comprising a transgene encoding beta-Glucocerebrosidase (GBA) or a portion thereof, Lysosomal Membrane Protein 2 (LIMP2), Prosaposin, or any combination of the foregoing. In some embodiments, the disclosure provides methods of Parkinson's disease by administering such expression constructs to a subject in need thereof. | 2021-10-28 |
20210332386 | EXTRACELLULAR VESICLES FOR TARGETED THERAPIES AGAINST MYELOID-DERIVED SUPPRESSOR CELLS - Disclosed herein are MDSC-targeted extracellular vesicles (EVs) loaded with therapeutic cargo, as well as compositions, systems, and methods for making same. Also disclosed herein is an MDSC-targeting ligand, such as a fusion protein containing an MDSC-targeting moiety. Also disclosed are EVs containing the disclosed fusion protein. In some embodiments, the EV is also loaded with a therapeutic cargo. Also disclosed is an EV-producing cell engineered to produce the disclosed EVs. Also disclosed is a method for making the disclosed EVs that involves culturing the disclosed EV-producing cells under conditions suitable to produce EVs. | 2021-10-28 |
20210332387 | GENETIC DATA COMPRESSION AND METHODS OF USE - Provided herein are genetically modified cells and methods of their production, wherein such methods include introducing a nucleic acid molecule including a plurality of index sequences into a cell comprising a synthetic landing pad, wherein each of the plurality of index sequences includes a first portion of a sequence and the synthetic landing pad includes a second portion of the sequence. The method further includes generating a plurality of cells that include the synthetic landing pad and the nucleic acid molecule including the plurality of index sequences and integrating one of the plurality of index sequences into the synthetic landing pad in each of the cells, thereby linking the first and second portions of the sequence. The linked first and second portions of the sequence result in a functional gene and cells including the integrated index sequence are selected based on presence or activity of the functional gene. | 2021-10-28 |
20210332388 | COMPOSITIONS, METHODS, MODULES AND INSTRUMENTS FOR AUTOMATED NUCLEIC ACID-GUIDED NUCLEASE EDITING IN MAMMALIAN CELLS - This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. | 2021-10-28 |
20210332389 | COMPOSITIONS, METHODS, MODULES AND INSTRUMENTS FOR AUTOMATED NUCLEIC ACID-GUIDED NUCLEASE EDITING IN MAMMALIAN CELLS USING MICROCARRIERS - This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. | 2021-10-28 |
20210332390 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2021-10-28 |
20210332391 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2021-10-28 |
20210332392 | COMPOSITIONS AND METHODS OF USE THEREOF FOR SCANDIUM SEPARATION FROM RARE EARTH CONTAINING MATERIAL - This disclosure provides microbes for the preferential separation of Scandium (Sc) from rare earth element (REE) containing materials, as well as methods of use thereof. | 2021-10-28 |
20210332393 | MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS - The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention. | 2021-10-28 |
20210332394 | METHOD FOR PRODUCING ORGANIC SUBSTANCE - Provided is a method which enables efficient separation of a component such as microorganisms from an organic substance-containing liquid obtained by microbial fermentation. Disclosed is a method for producing an organic substance comprising a microbial fermentation step of obtaining an organic substance-containing liquid and a separation step of heating the organic substance-containing liquid and separating into a liquid or solid component containing microorganisms and a gaseous component containing the organic substance. | 2021-10-28 |
20210332395 | POLYHYDROXYALKANOATE PRODUCTION METHODS AND SYSTEMS FOR SAME - Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products. | 2021-10-28 |
20210332396 | GENETIC ELEMENTS IN ENTEROCOCCUS SPP. TO PRODUCE DOPAMINE - The present invention relates to nucleic acid molecules from regions of | 2021-10-28 |
20210332397 | METHOD FOR PREPARING NATURAL DELTA-DECALACTONE AND DELTA-DODECALACTONE BY BIOREDUCTION OF MASSOIA OIL - The present disclosure discloses a method for preparing natural delta-decalactone and delta-dodecalactone by bioreduction of Massoia Oil, which comprises (1) Strain activation, (2) Preparation of seed culture solution, (3) Fermentation and conversion, (4) Extraction, (5) Solvent recycle, and (6) Rectification. | 2021-10-28 |
20210332398 | GENETICALLY MODIFIED BACTERIAL CELLS AND METHODS USEFUL FOR PRODUCING INDIGOIDINE - The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes. | 2021-10-28 |
20210332399 | TRANSAMINASE BIOCATALYSTS - The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides. | 2021-10-28 |
20210332400 | Nucleic Acid Amplification - Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers. | 2021-10-28 |
20210332401 | METHOD FOR ENHANCING EXTRACELLULAR VESICLE PRODUCTION - A method for enhancing extracellular vesicle production is described. A peptide that induces polymer formation is incubated with a cell culture which results in enhanced EV production. The peptide penetrates the cells and subsequently polymerizes upon exposure to enzymes (e.g. phosphatase) within the cell. The cells that contain the newly formed polymers have an increased production of EVs. These EVs can be harvested using centrifugation techniques. | 2021-10-28 |
20210332402 | SERUM-FREE CELL CULTURE MEDIUM - The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest. | 2021-10-28 |
20210332403 | ENGINEERED AND FULLY-FUNCTIONAL CUSTOMIZED GLYCOPROTEINS - Described herein are compositions and methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof. | 2021-10-28 |
20210332404 | LIPID NANOPARTICLES FOR MEASURING CHRONIC AND ACUTE RESPONSE TO EXERCISE - The invention relates to biosensor for detecting and/or quantifying exercise and disease-risk associated enzymes in a biological sample and related methods. The biosensor includes a solid support and a nanostructure with a metallic core and a phospholipid shell capable of binding to a lecithin:cholesterol acyltransferase (LCAT) activator (BL-NP). | 2021-10-28 |
20210332405 | METHODS FOR IMMUNOASSAYS USING ELECTROCHEMICAL MEASUREMENT - Disclosed herein is a method for detecting a binding event between an immobilised primary antibody and a target analyte-secondary antibody-enzyme complex using an electrochemical measurement technique. | 2021-10-28 |
20210332406 | SEROTYPE DETERMINATION METHOD - The present invention provides a novel serotype determination method for | 2021-10-28 |
20210332407 | METHOD FOR MEASURING THE CONTAMINATION OF DRINKING WATER BY MICRO-ORGANISMS, IN A DRINKING WATER CONDUIT - The invention is based on a method, particularly a locally fixed method, for measuring a contamination of drinking water by microorganisms ( | 2021-10-28 |
20210332408 | SYSTEMS AND METHODS FOR OPTIMIZATION OF ENZYMES - Poly and perfluoroalkyl substances (PFAs) are extremely resistant to natural degradation. Described herein are compositions, assays, and methods for generating microorganisms capable of accelerating degradation of these products using a directed evolution strategy. A minimal media including the target carbon source of interest, creates selective pressure for microorganisms capable of degradation. Extremely slow growth rates on these alternative carbon sources is nevertheless measureable using automated image capture and processing pipeline allows one to observe very small changes in cell growth over the course of a very long period of time, thereby identifying microorganisms that could not otherwise be identified. | 2021-10-28 |
20210332409 | INHIBITION AND TREATMENT OF GASTROINTESTINAL BIOFILMS - Orally administered physiologically acceptable anti-biofilm compositions comprising enzymes and if desired additional components such as antimicrobials, antibiotics, antifungals, herbals, chelating agents, lactoferrin and related compounds, minerals, surfactants, binders, and fillers useful for the inhibition and treatment of gastrointestinal biofilms in humans. Physiologically acceptable anti-biofilm compositions containing these enzymes are useful in the inhibition, reduction and/or treatment of gastrointestinal biofilm infections, and associated systemic symptoms caused by biofilms associated microorganisms within the gastrointestinal tract. Methods of identification, preparation and use of such physiologically acceptable anti-biofilm compositions are also provided. | 2021-10-28 |
20210332410 | BLOOD SAMPLE ASSAY METHOD - The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided. | 2021-10-28 |
20210332411 | METHODS AND REAGENT FOR ANALYSING NUCLEIC ACIDS FROM INDIVIDUAL CELLS - The present disclosure relates to a bispecific reagent and a detection agent which are useful for the identification of an antibody-producing cell which produces an antibody that binds specifically to a target antigen. Further, the present disclosure relates to an assay for the identification of an antibody-producing cell which produces an antibody that binds specifically to a target antigen. The present disclosure also relates to kits comprising the bispecific reagent and the detection agent. | 2021-10-28 |
20210332412 | HOMOPOLYMER PRIMERS FOR AMPLIFICATION OF POLYNUCLEOTIDES CREATED BY ENZYMATIC SYNTHESIS - This disclosure describes a technique for performing random access in a pool of polynucleotides by using one unique primer and one homopolymer primer to selectively amplify some but not all of the polynucleotides in the pool. The polynucleotides are synthesized by a template independent polymerase such as terminal deoxynucleotide transferase (TdT) rather than by phosphoramidite synthesis. Enzymatic synthesis efficiently creates homopolymer sequences through unregulated synthesis. Use of one homopolymer primer instead of two unique primers decreases the complexity, time, and cost of synthesizing the polynucleotides. Use of a unique primer provides a sequence that can be varied to uniquely identify multiple different groups of polynucleotides. This enables random access by polymerase chain reaction (PCR) amplification while still benefitting from the efficiency of homopolymer synthesis. The polynucleotides may include payload regions that use a sequence of nucleotides to encode digital data. | 2021-10-28 |
20210332413 | METHODS AND COMPOSITIONS FOR GENOMIC TARGET ENRICHMENT AND SELECTIVE DNA SEQUENCING - It has been established that one or more large double stranded DNA fragments (each 2,000 to 40,000 base pairs in size) can be captured and isolated from genomic DNA fragments using sequence specific PNA hybridization probes. Compositions and methods for enrichment of a multiplicity of long DNA sequences selected from the genome of any eukaryote are provided. Capture is performed using multiple PNA molecules with gamma-modified chiral backbones, comprising a mixture of neutral and positive chemical groups. Two or more PNA probes with covalently bound haptens, preferably biotin, target each DNA domain of interest for capture, isolation, and subsequent sequencing analysis of the multiplicity of enriched targets, including DNA methylation sequencing. The methods include enhancement of probe-DNA binding specificity through single strand binding proteins (SSB). | 2021-10-28 |
20210332414 | COMPOSITIONS AND METHODS FOR ANALYTE DETECTION - Methods of making a three-dimensional matrix of nucleic acids within a cell is provided. | 2021-10-28 |
20210332415 | COMPOSITIONS AND METHODS FOR ANALYTE DETECTION - Methods of making a three-dimensional matrix of nucleic acids within a cell is provided. | 2021-10-28 |
20210332416 | HIGH PERFORMANCE FLUORESCENCE IMAGING MODULE FOR GENOMIC TESTING ASSAY - Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications. | 2021-10-28 |
20210332417 | Molecular Marker For Identifying Sanxan Gum and Its Synthetic Strain, Preparation Method And Application Thereof - The present invention relates to microorganism identification, and more particularly to a molecular marker for identifying Sanxan gum and its synthetic strain, and preparation method and application thereof. The molecular marker is prepared by PCR amplification using genomic DNA of | 2021-10-28 |
20210332418 | SYSTEMS AND METHODS FOR MICROBIOME ANALYSIS - Provided are methods balancing a gut microbiome. Methods involve sequencing nucleic acids from a sample to determine quantifies or activities of microorganisms Sequence information is used to generate functional activity scores for functional categories. These scores can be actionable. Methods also include generating recommendations involving administration of food, supplements, or medication to affect quantities or activities of microorganisms | 2021-10-28 |
20210332419 | MONITORING HOST CELL CONTAMINATION OF VIRUS-BASED BIOLOGICAL PRODUCTS - The invention relates to the production of biopharmaceuticals and, more particularly, to a novel method of monitoring for contamination of such products with components of any host cells used or involved in the manufacturing process. The method comprises a step of determining the presence in a biopharmaceutical (e.g. a therapeutic phage composition) of a ribosomal subunit protein unique to bacteria (or protein fragments thereof) or a nucleic acid molecule comprising a nucleotide sequence derived from a gene encoding a ribosomal subunit protein unique to bacteria. | 2021-10-28 |
20210332420 | NANO-VESICLE DERIVED FROM CORYNEBACTERIUM SP. BACTERIA AND USE THEREOF - Provided are vesicles derived from bacteria of the genus | 2021-10-28 |
20210332421 | MULTIPLEX DETECTION OF VULVOVAGINAL CANDIDIASIS, TRICHOMONIASIS AND BACTERIAL VAGINOSIS - Methods and compositions for detection of vulvovaginal candidiasis (VVC), trichomoniasis and bacterial vaginosis (BV) are disclosed herein. In some embodiments, the presence or absence of VVC-associated | 2021-10-28 |
20210332422 | METHODS OF PREDICTING SUSCEPTIBILITY TO INFECTIOUS DISEASE AND RELATED METHODS OF TREATMENT - Disclosed herein are methods for predicting susceptibility to an infection based on the observation of specific mutations in blood cells, as well as methods for treating or reducing susceptibility to an infection in a subject. | 2021-10-28 |
20210332423 | HIGHLY STABLE AND SPECIFIC MOLECULAR BEACONS ENCAPSULATED IN CATIONIC LIPOPLEX NANOPARTICLES AND APPLICATION THEREOF - The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention. | 2021-10-28 |
20210332424 | METHODS OF GENERATING AN ARRAY - This disclosure relates to compositions and methods for generating a spatial array. | 2021-10-28 |
20210332425 | INCREASING SPATIAL ARRAY RESOLUTION - This disclosure relates to methods of manufacturing of spatial arrays and determining the location of an analyte present in a biological sample (e.g., by utilizing a cell-tagging agent). | 2021-10-28 |