Entries |
Document | Title | Date |
20080213860 | Nicking Endonuclease Methods and Compositions - A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or gutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6. | 09-04-2008 |
20080220498 | Variant Buttiauxella sp. phytases having altered properties - The present invention relates to variant phytase enzymes having altered properties. | 09-11-2008 |
20090004720 | Smart Biocatalysts For Organic Synthesis - The present disclosure relates to compositions, systems, and methods that include a reusable biocatalyst. A reusable biocatalytic composition may include a stimulus-responsive support operable to be soluble under at least one first condition and insoluble under at least one second condition; and a biocatalyst bound to the stimulus-responsive support complex. According to some embodiments, a method for catalyzing a reaction may include contacting a first reactant with a composition comprising a stimulus-responsive support, a biocatalyst linked to the stimulus-responsive support, and a first solvent under conditions in which the stimulus-responsive support is soluble in the solvent and the reactant is converted to a product. A reusable biocatalyst system, in some embodiments, may include (a) a stimulus-responsive support; (b) a biocatalyst (i) bound to the stimulus-responsive support complex and (ii) having catalytic activity in the presence of a substrate; and a solvent operable to support both first and second condition. | 01-01-2009 |
20090081761 | Modified hydrogenase, enzymatic electrode made of modified hydrogenase, and hydrogenase modification method - The invention provides: (1) a modified hydrogenase obtained by removing electron-transfer sites from a hydrogenase constituted of: active subunits including active sites having a hydrogen oxidization-reduction activity; and electron-transfer subunits having the electron-transfer sites through which electrons are transferred between the active sites and the outside of the hydrogenase; (2) a modified hydrogenase obtained from a hydrogenase, wherein when the hydrogenase is isolated from bacteria that produces the hydrogenase, a process for exposing the hydrogenase to an oxygen atmosphere is executed; (3) an enzymatic electrode made of at least one of the foregoing modified hydrogenases; and (4) a hydrogenase modification method including: a step of isolating from hydrogenase-producing bacteria; and a step of removing the electron-transfer sites of the electron-transfer subunits from the hydrogenase by exposing the hydrogenase to an oxygen atmosphere. | 03-26-2009 |
20090123987 | Extracting and purifying limit dextrinases - The invention relates to improved methods for extracting and purifying limit dextrinase enzymes from cells, in particular plant cells. The process of the invention includes heating a cell homogenate at 4O° C., in the presence of divalent cations thus increasing the specific activity of the limit dextrinase. | 05-14-2009 |
20090162915 | Peptidylarginine Deiminase 6 - A nucleotide acid sequence is provided encoding a peptidylarginine deiminase 6. The gene is found to be expressed in gonads only and may be used as target for male and female contraception. Its encoded protein can be used to screen for small molecular weight modulators of the enzyme activity. | 06-25-2009 |
20090191607 | SYNTHETIC ENZYMES DERIVED FROM COMPUTATIONAL DESIGN - Disclosed herein are techniques for computationally designing enzymes. These techniques can be used to design variations of naturally occurring enzymes, as well as new enzymes having no natural counterparts. The techniques are based on first identifying functional reactive sites required to promote the desired reaction. Then, hashing algorithms are used to identify potential protein backbone structures (i.e., scaffolds) capable of supporting the required functional sites. These techniques were used to design 32 different protein sequences that exhibited aldol reaction catalytic function, 31 of which are defined in the Sequence Listing. Details of these 31 different synthetic aldolases are provided, including descriptions of how such synthetic aldolases can be differentiated from naturally occurring aldolases. | 07-30-2009 |
20090325266 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 12-31-2009 |
20100129892 | PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICRO-ORGANISM IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS - The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter. | 05-27-2010 |
20100144010 | Enzymatic Asymmetric Decarboxylation of Disubstituted Malonic Acids - A process for the stereoselective decarboxylation of malonic acid derivatives with mutated decarboxylases is disclosed. | 06-10-2010 |
20100261250 | Cyanide-Tolerant Nitrile Hydratases - Disclosed herein are cyanide-tolerant nitrile hydratases especially from | 10-14-2010 |
20100261251 | MICROBIAL KINETIC RESOLUTION OF ETHYL-3,4-EPOXYBUTYRATE - Two novel microorganisms, | 10-14-2010 |
20100279380 | Methods for Degrading Toxic Compounds - The invention relates to bacteria, bacterial extracts, supernatants obtained from the culturing of said bacteria, polypeptides and compositions for degrading benzimidazole carbamate fungicides, carbanilate fungicides, sulfonamide herbicides, thioamide herbicides and/or synthetic pyrethroid insecticides. In particular, the invention relates to the identification of | 11-04-2010 |
20100285567 | TAT SIGNAL PEPTIDES FOR PRODUCING PROTEINS IN PROKARYOTES - This invention provides polynucleotides encoding TAT fusion proteins, and methods for producing proteins of interest in a host cell. In particular, the present invention relates to polynucleotides, vectors, polypeptides and methods for expressing organophosphate-degrading enzymes e.g. organophosphorus hydrolase (OPH) in host cell, such as a | 11-11-2010 |
20110059508 | IMPROVING AGENT FOR DYSFUNCTION DUE TO NEUROPATHY AND RHO KINASE ACTIVATION INHIBITOR - An object of the present invention is to provide a substance which is able to be an active ingredient for the improvement of dysfunction caused by nerve damage. An improving agent for dysfunction due to nerve damage of the present invention as a means for resolution thereof is characterized in that it comprises an endo-β-N-acetylglucosaminidase type enzyme which hydrolyzes an N-acetylglucosamide bond in a keratan sulfate backbone as an active ingredient. When the improving agent of the present invention is administered, clinical improvement is achieved in motor neuron dysfunction and sensory neuron dysfunction such as neuropathic pain represented by a pain caused by allodynia and hyperalgesic reaction of the object to be treated. | 03-10-2011 |
20110287511 | Novel Trichoderma Genes - Described herein are novel gene sequences isolated from | 11-24-2011 |
20120135497 | DIFFERENTIALLY FLUORESCENT YEAST BIOSENSORS FOR THE DETECTION AND BIODEGRADATION OF CHEMICAL AGENTS - The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and/or detect the modified form of the chemical agent. The present disclosure also relates, in some embodiments, to variant organophosphorus hydrolase having one or more desirable amino acid substitutions. | 05-31-2012 |
20120178143 | ALPHA/BETA HYDROLASE-FOLD ENZYMES - The present invention relates to the identification of novel hydrolases in gram positive microorganisms. The present invention provides amino acid sequences for the hydrolase. The present invention provides host cells which comprise nucleic acid encoding the hydrolase. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the hydrolase of the present invention. | 07-12-2012 |
20120220013 | COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 08-30-2012 |
20120329133 | PROCESS FOR PURIFYING HEPARAN-N-SULFATASE - A process for preparing and purifying heparan-N-sulfatase is disclosed involving chromatographic steps for producing or purifying heparan-N-sulfatase under conditions that yield highly pure heparan-N-sulfatase. | 12-27-2012 |
20130115675 | Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature. | 05-09-2013 |
20130177963 | ASYMMETRIC HYDROLASE AND GENE THEREOF - The present invention relates to, for example, an α-substituted β-amino acid ester derivative asymmetric hydrolase including an enzyme of the following (a) or (b): | 07-11-2013 |
20130183738 | TRANSGENIC PLANTS MODIFIED FOR REDUCED CADMIUM TRANSPORT, DERIVATIVE PRODUCTS, AND RELATED METHODS - Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters. | 07-18-2013 |
20130302880 | POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION - The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins. | 11-14-2013 |
20130337539 | COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 12-19-2013 |
20130344565 | Optimization of Expression and Purification of Recombinant Human MxA Protein in E. Coli - Full length MxA constructs and truncated MxA constructs produce human MxA protein in | 12-26-2013 |
20140322794 | SUBSTRATES FOR COVALENT TETHERING OF PROTEINS TO FUNCTIONAL GROUPS OR SOLID SURFACES - The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates. | 10-30-2014 |
20150140636 | POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION - The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins. | 05-21-2015 |
20160090581 | RECOMBINASE MUTANTS - Presented herein are recombinases for improved recombinase-mediated amplification of nucleic acids, such as a PCR-library having single-stranded adapter regions, on a patterned flow cell surface for improved cluster amplification, as well as methods and kits using the same. | 03-31-2016 |