Entries |
Document | Title | Date |
20080206834 | RECOMBINANT ORGANOPHOSPHOROUS ACID ANHYDRASE AND METHODS OF USE - The bacterial gene (opd) encodes an organophosphorus anhydrase which is capable of hydrolyzing a wide spectrum of neurotoxins ranging from insecticides to mammalian neurotoxins. The cloned gene has been expressed in a number of hosts and the purified enzyme has been characterized. These advances have led to a number of interrelated uses for the cloned gene and the recombinant enzyme including: detoxification of organophosphorus compounds; detection of organophosphorus compounds; protection of susceptible organisms from organophosphorus poisoning; and, detection of organophosphorus-detoxifying microorganisms. | 08-28-2008 |
20080248550 | ENHANCEMENT OF VANADIUM-CONTAINING PHOSPHATASE INHIBITORS - The present invention is directed to a composition comprising a vanadium-containing phosphatase inhibitor and a polyol. In the presence of the polyol the effect of the inhibitor is enhanced, even in the presence of chelating agents or reducing agents. The invention also concerns the use of the inventive composition for inhibiting a phosphatase, as well as kits comprising the composition. | 10-09-2008 |
20080311641 | Crystallization of enzyme and method for producing same - The present invention provides a method of crystallizing of enzymes. The method is for rapidly crystallizing enzymes from impure mixtures. The method is simple and cheap, and it is compatible to industrial requirements. T1 lipase was able to form crystals at low protein concentration (2.5 mg/ml) in a day. High temperature crystallization was obtained from the method. The present invention also relates to a composition of a crystallized lipase produced from the said method. | 12-18-2008 |
20090197318 | CRYSTALLIZATION OF ENZYME AND METHOD FOR PRODUCING SAME - The present invention provides a method of crystallizing of enzymes. The method is for rapidly crystallizing enzymes from impure mixtures. The method is simple and cheap, and it is compatible to industrial requirements. T1 lipase was able to form crystals at low protein concentration (2.5 mg/ml) in a day. High temperature crystallization was obtained from the method. The present invention also relates to a composition of a crystallized lipase produced from the said method. | 08-06-2009 |
20090258406 | Use of esterases for separating plastics - The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof. | 10-15-2009 |
20100068786 | METHODS AND COMPOSITIONS FOR REVERSING P-GLYCOPROTEIN MEDICATED DRUG RESISTANCE - A method for inhibiting therapeutic drug resistance within a cell over-expressing a membrane protein is provided. The method comprises synthesizing a dimeric prodrug inhibitor of a monomeric therapeutic agent; administering the dimeric prodrug inhibitor to the membrane protein together with the monomeric therapeutic agent; and occupying at least one substrate binding site of the membrane protein with the synthesized dimeric prodrug to allow the monomeric therapeutic agent to accumulate within the cell. The dimeric prodrug inhibitor contains a crosslinking agent that is adapted to breakdown under reducing conditions within the cytosol of the cell to cause the dimeric prodrug to revert back to a form equivalent to the monomeric therapeutic agent. | 03-18-2010 |
20100099161 | Alpha-Amylase Mutants With Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca | 04-22-2010 |
20100112662 | MICROORGANISM FOR PRODUCING RECOMBINANT PIG LIVER ESTERASE - The invention relates to a method for the processing of reaction solutions, containing whole-cell catalysts, an aqueous component and an organic component, wherein the organic component contains the product to be enriched, with the following steps: a) adjustment of the pH value to less than 4; b) filtration of the reaction solution in the presence of a filter aid, preferably in the reaction solution; c) optionally: further enrichment and/or purification of the product contained in the organic component. | 05-06-2010 |
20100144011 | CRYSTAL GROWTH UNDER MICROGRAVITY CONDITION - The present invention relates to the growth of protein crystallization on earth and in zero-gravity conditions. Moreover, the invention relates to preferred conditions to develop protein crystals or 3D crystal structures. Furthermore, the preferred embodiment of the present invention relates to an evaluation of 3D crystal growth under microgravity (zero-gravity) and on earth. In addition, the invention also relates to the quality of protein crystal/s grown on earth and zero-gravity. The present invention provides a new and improved method for growing protein crystals and for screening crystallization conditions in solution crystal growth. | 06-10-2010 |
20100144012 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide. | 06-10-2010 |
20100304462 | Synthetic Binding Proteins - Methods and compositions are provided for creating a binding protein that recognizes a rationally chosen recognition sequence in which a first amino acid has been substituted for a second amino acid using site-directed mutagenesis of a member protein of a set of proteins at an identified position or positions correlated with recognition of a chosen specified target module in the recognition sequence. A system is provided for automating the storage and manipulation of the correlations between positions and types of amino acid residues in the binding protein with specific modules at specified positions in the target recognition sequence and for designing and creating proteins with novel specificities. | 12-02-2010 |
20100323425 | DETECTION OF NUCLEIC ACID DIFFERENCES USING COMBINED ENDONUCLEASE CLEAVAGE AND LIGATION REACTIONS - The present invention is a method for detecting DNA sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined. | 12-23-2010 |
20110053246 | OVEREXPRESSION OF PHYTASE GENES IN YEAST SYSTEMS - The present invention relates to a method of producing a heterologous protein or polypeptide having phytase activity in a yeast system. The invention also provides proteins having phytase activity which have increased thermostability. Yeast strains which produce a heterologous phytase and the vectors used to produce the phytase are also provided. | 03-03-2011 |
20110104785 | METHODS AND KITS FOR 3'-END-TAGGING OF RNA - The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5′-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides. | 05-05-2011 |
20110117625 | Engineered Nucleases and Their Uses for Nucleic Acid Assembly - Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA. | 05-19-2011 |
20110129897 | PROCESS FOR MAKING MICRO-SIZED PROTEIN PARTICLES - A process of making micro-sized protein particles comprising the step of drying nano-sized protein particles suspended in a liquid medium under conditions to agglomerate the nano-sized protein particles and thereby form micro-sized protein particles. | 06-02-2011 |
20110129898 | Methods and compositions for increasing nuclease activity - Disclosed herein are methods and compositions for increasing nuclease activity to increase nuclease-mediated genomic modifications. | 06-02-2011 |
20110151539 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide. | 06-23-2011 |
20110159569 | ANTI-NUCLEIC ACID ANTIBODY INDUCING CELL DEATH OF CANCER CELLS AND COMPOSITION FOR PREVENTING OR TREATING CANCERS COMPRISING THE SAME - There are provided an anti-nucleic acid antibody inducing cell death of cancer cells by invading normal cells; and a composition for preventing or treating cancers comprising the anti-nucleic acid antibody, which shows anticancer effect of an anti-nucleic acid antibody that shows cytotoxicity by damaging nucleic acid strands, that is, genetic information of a cancer cell when the anti-nucleic acid antibody, which has binding activity and degrading activity to the nucleic acid strands in cells at the same time, is overexpressed in the cancer cell, or flows in the cancer cell. Therefore, the composition for treating cancers may be useful to induce the selective cell death in cancer cells than in normal cells since the anti-nucleic acid antibody very easily permeates into the cancer cells due to the excellent selectivity, compared to the normal cells. | 06-30-2011 |
20110183400 | PROCESS FOR PRODUCTION OF LIPASES BY GENETIC MODIFICATION OF YEAST - The present invention relates to the construction of optimized synthetic lipase gene expression vectors for the high level expression of recombinant lipases in the yeast. The invention provides an enzymatic approach to the industrial processing of by-products resulting from biodiesel production. | 07-28-2011 |
20110207199 | NOVEL METHOD TO GENERATE MEGANUCLEASES WITH ALTERED CHARACTERISTICS - Method to generate and select a meganuclease having at least two altered characteristics in comparison to a parent meganuclease, comprising the steps: a. constructing from a parent meganuclease, a first series of variants which differ from said parent meganuclease by at least one acid amino substitution; b. screening the variants from said first series of step a. and selecting those which have a first altered characteristic; c. constructing from the selected variants of step b. a second series of variants having a least one other amino acid substitution; d. screening the variants from said series of step b. and selecting those which have said first altered characteristic and a second altered characteristic. Polypeptide obtained from said method. | 08-25-2011 |
20110256607 | HOMING ENDONUCLEASES - The present disclosure provides, in part, polypeptides having endonuclease activity, nucleic acid sequences for such a polypeptide, target sequences for the endonuclease, as well as vectors, cells, kits, methods, and uses of the same. | 10-20-2011 |
20110262996 | METHODS AND MATERIALS FOR DEGRADING PYRETHROID - Methods and compositions are provided for enzymatic detoxification of pyrethroid such as permethrin. The methods involve application of compositions of organophosphorus hydrolase OPH to surfaces or substances contaminated with pyrethroid such as permethrin. | 10-27-2011 |
20110287512 | Compositions for linking zinc finger modules - Disclosed herein are compositions for linking DNA binding modules to allow for specific and selective binding to module subsites separated by 1 or more base pairs. Also described are methods of making and using compositions comprising these linkers. | 11-24-2011 |
20110287513 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide. | 11-24-2011 |
20120015421 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 01-19-2012 |
20120021488 | THERMOTOLERANT NON-K12 ESCHERICHIA COLI PHYTASE AND ITS PRODUCTION - This invention relates to cloning and sequencing of thermotolerant phytase gene from Non-K12 | 01-26-2012 |
20120040434 | Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene - The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii) or (iii):
| 02-16-2012 |
20120100596 | DFPASE ENZYMES FROM OCTOPUS VULGARIS - The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 04-26-2012 |
20120107906 | NOVEL LIPOLYTIC ENZYME LIP1 - The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same. | 05-03-2012 |
20120129239 | HOMOLOGOUS RECOMBINATION-BASED DNA CLONING COMPOSITIONS - Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein. | 05-24-2012 |
20120142072 | SOLUBLE PROTEIN AND UTILIZATION OF THE SAME - Disclosed is a method for screening a medicine using a protein, and a compound obtained by the screening method. A compound or a salt thereof inhibiting activity or expression of the protein of the present invention, a neutralizing antibody against the protein, a polynucleotide that are complementary to a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating neurodegenerative diseases and the like. Moreover, a compound or a salt thereof enhancing activity or expression of the protein of the present invention, the protein or a partial peptide thereof, a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating cancers and the like. Furthermore, the protein of the present invention is useful as a reagent for screening a compound inhibiting or enhancing activity of the protein. | 06-07-2012 |
20120220014 | NOVEL POLYPEPTIDE HAVING ESTERASE ACTIVITY AND RECOMBINANT ESTERASE AND USE THEREOF - Polypeptide and recombinant protein having esterase activity which exhibit the amino acid sequence SEQ. ID. No. 1 and the use thereof. | 08-30-2012 |
20120225467 | MODIFIED DNASE COMPOSITIONS AND METHODS OF USE THEREOF - Modified DNase polypeptides and methods of their use are provided. | 09-06-2012 |
20120225468 | Hafnia Phytase Variants - The present invention relates to phytases having at least 76% identity to a phytase derived from | 09-06-2012 |
20120264189 | Rationally-Designed Meganucleases With Altered Sequence Specificity and DNA-Binding Affinity - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 10-18-2012 |
20120329134 | Enhancement of Vanadium-Containing Phosphatase Inhibitors - The present invention is directed to a composition comprising a vanadium-containing phosphatase inhibitor and a polyol. In the presence of the polyol the effect of the inhibitor is enhanced, even in the presence of chelating agents or reducing agents. The invention also concerns the use of the inventive composition for inhibiting a phosphatase, as well as kits comprising the composition. | 12-27-2012 |
20130029401 | Synthetic Binding Proteins - Methods and compositions are provided for creating a binding protein that recognizes a rationally chosen recognition sequence in which a first amino acid has been substituted for a second amino acid using site-directed mutagenesis of a member protein of a set of proteins at an identified position or positions correlated with recognition of a chosen specified target module in the recognition sequence. A system is provided for automating the storage and manipulation of the correlations between positions and types of amino acid residues in the binding protein with specific modules at specified positions in the target recognition sequence and for designing and creating proteins with novel specificities. | 01-31-2013 |
20130040366 | METAGENOME-DERIVED ALKALINE PHOSPHATASE - The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay. | 02-14-2013 |
20130040367 | PHOSPHOTRIESTERASE FROM AGROBACTERIUM RADIOBACTER P230 - The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from an | 02-14-2013 |
20130095553 | POLYPEPTIDE HAVING OR ASSISTING IN CARBOHYDRATE MATERIAL DEGRADING ACTIVITY AND USES THEREOF - The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins. | 04-18-2013 |
20130115676 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 05-09-2013 |
20130115677 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 05-09-2013 |
20130115678 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 05-09-2013 |
20130115679 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 05-09-2013 |
20130122567 | ENHANCED PHYTASE VARIANTS - The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and/or activity than the original phytase. The invention also provides a nucleic acid coding for said variant, a cassette or an expression vector containing said variant, a host cell expressing said variant, a composition comprising said variant and uses thereof, principally in the preparation of food additives and animal feed. | 05-16-2013 |
20130164819 | Method and Kit for Sequential Isolation of Nucleotide Species From a Sample - The invention provides a process and kit for serial isolation of DNA and RNA from the same sample. First, a siliceous solid support with preferential affinity for DNA over RNA is used to capture DNA in a lysate of a sample. Next, a siliceous solid support with similar affinity for RNA and DNA is used to capture RNA from the same lysate. The respective solid supports are recovered independent of each other, washed, and their bound nucleotide species are eluted. The invention further provides DNA and RNA prepared using the process in a minimal number of steps employing a minimal number of reagents. As the invention yields DNA and RNA of high quality and is amenable to automation, the invention may be used widely in the healthcare and pharmaceutical industries. | 06-27-2013 |
20130189759 | MEGANUCLEASES VARIANTS CLEAVING A DNA TARGET SEQUENCE IN THE NANOG GENE AND USES THEREOF - Meganuclease variants cleaving DNA target sequences of the NANOG gene, vectors encoding such variants, and cells expressing them. Methods of using meganuclease variants recognizing NANOG gene sequences for modifying the NANOG gene sequence or for incorporating a gene of interest or therapeutic gene using the NANOG gene as a landing pad and a safe harbor locus. | 07-25-2013 |
20130196410 | COMPOSITIONS AND METHODS FOR MODIFYING THE GLYCOSYLATION PATTERN OF A POLYPEPTIDE - Provided herein are methods and compositions for expressing a modified polypeptide in a host cell, wherein the modified polypeptide comprises a terminal mannose at an N-linked glycosylation site of the polypeptide. The methods and compositions used herein involve the use of RNA effector molecules (e.g., siRNA, dsRNA etc) administered to a host cell to modify the expression of target genes involved in protein glycosylation (e.g., Mgat1, Mgat4, SLC35A1, SLC35A2 or GNE). | 08-01-2013 |
20130224832 | Novel Restriction Endonucleases, DNA Encoding These Endonucleases and Methods for Identifying New Endonucleases with the Same or Varied Specificity - Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e−02. | 08-29-2013 |
20130236946 | MEGANUCLEASE VARIANTS CLEAVING A DNA TARGET SEQUENCE FROM THE MOUSE ROSA26 LOCUS AND USES THEREOF - An I-CreI variant, wherein one of the two I-CreI monomers has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG core domain situated respectively from positions 26 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from the mouse ROSA26 locus. Use of said variant and derived products for the engineering of transgenic mice and recombinant mouse cell lines expressing an heterologous protein of interest. | 09-12-2013 |
20130244303 | OVEREXPRESSION OF PHYTASE GENES IN YEAST SYSTEMS - The present invention relates to a method of producing a heterologous protein or polypeptide having phytase activity in a yeast system. The invention also provides proteins having phytase activity which have increased thermostability. Yeast strains which produce a heterologous phytase and the vectors used to produce the phytase are also provided. | 09-19-2013 |
20130280786 | ENZYMES AND USES THEREOF - The present invention relates to novel enzymes and the uses thereof. The invention also relates to methods of producing such enzymes, coding nucleic acid molecules, recombinant cells and methods of transforming biomass from such materials. The invention is particularly suited to degrade biomass and/or to improve biomass degradation, and to produce bioenergy products or recombinant proteins. This invention also relates to various applications of the enzymes in the field of paper industry, textile industry as well as in the chemical and medical fields. | 10-24-2013 |
20130344566 | Inotropic Antibodies And Therapeutic Uses Thereof - Antibodies binding to sites on the alpha-subunit of the (Na | 12-26-2013 |
20140004593 | CELLS FOR PRODUCING RECOMBINANT IDURONATE-2-SULFATASE | 01-02-2014 |
20140030790 | ALKALINE PHOSPHATASE - The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus | 01-30-2014 |
20140154778 | High Fidelity Restriction Endonucleases - Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer. | 06-05-2014 |
20140154779 | High Fidelity Restriction Endonucleases - Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer. | 06-05-2014 |
20140186919 | ENGINEERING AND OPTIMIZATION OF IMPROVED SYSTEMS, METHODS AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION - The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme. | 07-03-2014 |
20140186920 | MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS - A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from | 07-03-2014 |
20140193878 | MRNA Interferase from Myxococcus Xanthus - A regulated deployment of a toxin gene for developmental programmed cell death in bacteria is described. | 07-10-2014 |
20140193879 | DFPASE ENZYMES FROM APLYSIA CALIFORNICA - The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 07-10-2014 |
20140234941 | PHOSPHATIDIC ACID PHOSPHATASE GENE - The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof. | 08-21-2014 |
20140335592 | TAL EFFECTOR-MEDIATED DNA MODIFICATION - Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided. | 11-13-2014 |
20150024464 | ENGINEERED NUCLEASES AND THEIR USES FOR NUCLEIC ACID ASSEMBLY - Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA. | 01-22-2015 |
20150031110 | PHYTASE HAVING IMPROVED ENZYMATIC ACTIVITY - A phytase having improved enzymaic activity is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Valine at position 90 with Threonine. Alternatively, the phytase has a modified amino acid sequence of SEQ ID NO: 6, wherein the modification is a substitution of Asparagine at position 204 with Alanine or a substitution of Serine at position 206 with Alanine. | 01-29-2015 |
20150037870 | ENZYME CATALYSTS FOR DIELS-ALDER REACTIONS - The present invention provides enzyme catalysts for Diels-Alder reactions, including intermolecular Diels-Alder reactions, as well as protein scaffolds for making such enzyme catalysts. In other aspects, the invention provides methods of making the enzyme catalysts, including by de novo computational design. The present invention thereby provides enzyme catalysts capable of catalyzing a desired Diels-Alder reaction, including with a specified or desired stereo-selectivity. | 02-05-2015 |
20150037871 | THERAPEUTIC NUCLEASE COMPOSITIONS AND METHODS - Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal. | 02-05-2015 |
20150132821 | Systems and Methods for Improving Nuclease Specificity and Activity - Endonucleases play an essential role in genetic engineering and molecular biology. A major barrier to the clinical adoption of nucleases and engineered nucleases remain the overall lack of specificity and activity. Off-site cleavage, cleavage at loci other than the target loci, typically occurs resulting in mutations, unexpected gene-knockouts, or translocations. Provided herein are systems and methods for identifying the off-site cleavage loci and predicting the activity of engineered endonucleases for a given genome. It is expected that these tools and methods will be useful for designing nucleases and other related DNA binding domains (e.g. TAL effectors) for genomic therapy and engineering. | 05-14-2015 |
20150147799 | HALOGEN-FREE HIGH-FREQUENCY RESIN COMPOSITION - Disclosed is a halogen-free high-frequency resin composition calculated according to parts by weight, and including 20-50 parts by weight of dicyclopentadiene epoxy resin, 10-40 parts by weight of styrene-maleic anhydride copolymer, 10-30 parts by weight of benzoxazine resin, 5-20 parts by weight of polyfunctional epoxy resin and 20-40 parts by weight of at least one phosphorus-containing flame retardant. A copper clad laminate made of the halogen-free high-frequency resin composition has excellent properties including a low dielectric constant, a low dielectric loss, a high heat resistance, a low water absorption, a low coefficient of expansion and a high PCB manufacturability. | 05-28-2015 |
20150353597 | Intein Mediated Purification of Protein - The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector. | 12-10-2015 |
20160053238 | CELLS FOR PRODUCING RECOMBINANT IDURONATE-2-SULFATASE - The present invention provides, among other things, methods and compositions for production of recombinant I2S protein with improved potency and activity using cells co-express I2S and FGE protein. In some embodiments, cells according to the present invention are engineered to simultaneously over-express recombinant I2S and FGE proteins. Cells according to the invention are adaptable to various cell culture conditions. In some embodiments, cells of the present invention adaptable to a large-scale suspension serum-free culture. | 02-25-2016 |
20160083700 | PHYTASE - Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning. | 03-24-2016 |
20160097053 | YEAST PROMOTERS FROM PICHIA PASTORIS - In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from | 04-07-2016 |
20160145589 | COMPOSITION AND FORMULATION COMPRISING RECOMBINANT HUMAN IDURONATE-2-SULFATASE AND PREPARATION METHOD THEREOF - A composition comprising recombinant iduronate-2-sulfatase (IDS) and a method for producing a purified recombinant IDS are provided. The glycosylation pattern and formylglycine content of the IDS composition are different from those of ELAPRASE® and have superior pharmaceutical efficacy and are safer than the conventional agent and thus can be effectively used for the therapy of Hunter syndrome. | 05-26-2016 |
20160194618 | PHYTASE | 07-07-2016 |
20160251640 | Compositions And Methods Of Nucleic Acid-Targeting Nucleic Acids | 09-01-2016 |
20170233704 | PRODUCTION FOR RECOMBINANT HUMAN DNASE I | 08-17-2017 |