Class / Patent application number | Description | Number of patent applications / Date published |
435209000 | Acting on beta-1, 4-glucosidic bond (e.g., cellulase, etc. (3.2.1.4)) | 80 |
20080199938 | Novel Cellulase Resistant to Surfactant - Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions. | 08-21-2008 |
20080206836 | Cellulase Variants - The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:
| 08-28-2008 |
20090081762 | Superactive Cellulase Formulation Using Cellobiohydrolase-1 from Penicillium Funiculosum - Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from | 03-26-2009 |
20090111160 | COMPOSITIONS COMPRISING BOWMAN-BIRK PROTEASE INHIBITORS AND VARIANTS THEREOF - The present invention provides compositions and methods related to expression of protease inhibitors and variants thereof in bacterial species. The present invention further provides fusion nucleic acids, vectors, fusion polypeptides, and processes for obtaining the protease inhibitors. | 04-30-2009 |
20090162916 | CELLOBIOHYDROLASE I ENZYMES - Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides. | 06-25-2009 |
20090170181 | EGVII Endoglucanase and Nucleic Acids Encoding The Same - The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same. | 07-02-2009 |
20090176292 | BGL7 Beta-Glucosidase and Nucleic Acids Encoding The Same - The present invention provides BGL7 polypeptides with the biological activity of a β-glucosidase and a method of producing a recombinant enzyme having β-glucosidase activity. | 07-09-2009 |
20100062511 | MODIFIED ENZYMES, METHODS TO PRODUCE MODIFIED ENZYMES AND USES THEREOF - The invention is directed to modified xylanases having increased stability in harsh industrial environments, such as increased pH and/or temperature. | 03-11-2010 |
20100167376 | METHODS FOR PROVIDING CELLULAR LYSATES FROM CELL WALL-CONTAINING SAMPLES - The present invention provides methods useful for making lysates from cell wall-containing cellular samples, including plant tissue samples and cultures of yeast or bacteria. The invention further provides compositions (e.g., solutions) that can be used in the methods of the invention, and kits comprising solutions and/or other reagents useful for carrying out the methods of the invention. | 07-01-2010 |
20100173386 | PROKARYOTIC EXPANSIN PROTEIN ACTIVATING CELLULOSE EXPANSION AND CELLULOSE - DEGRADING COMPOSITION COMPRISING THE SAME - Provided is a prokaryotic expansin protein capable of expanding cellulose. The prokaryotic expansin protein performs the same function as existing plant expansin proteins. The prokaryotic expansin protein can be produced at greatly reduced cost on an industrial scale, unlike plant expansin proteins. Particularly, when lignocellulosic biomass is hydrolyzed into glucose using the prokaryotic expansin protein and cellulase, the hydrolysis rate of cellulose can be markedly increased by the cellulase, resulting in improved yield of the glucose. In actuality, the use of the prokaryotic expansin enables the production of bioenergy at low cost with a reduced amount of enzyme used. The prokaryotic expansin protein softens the textures of pulp, cotton fibers (e.g., jeans), etc. Therefore, the prokaryotic expansin protein can be used for various purposes, such as biopulping and biostoning. | 07-08-2010 |
20100203615 | Polypeptides having endoglucanase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-12-2010 |
20100304464 | STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASES - The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides. | 12-02-2010 |
20100323426 | Heterologous and Homologous Cellulase Expression System - The present invention provides filamentous fungi that express a combination of heterologous and homologous polypeptides, polypeptide mixtures comprising a combination of heterologous and homologous polypeptides and methods of producing the polypeptide mixtures. | 12-23-2010 |
20100330648 | METHOD AND SYSTEM FOR PREPARING BIOMASS FOR BIOTREATMENT IN A STATIC SOLID STATE BIOREACTOR - A method and system for preparing biomass for biotreatment in a static solid state bioreactor is performed in two stages. The first stage includes pre-mixing of the biomass with one or more reagent(s). The second stage includes the addition of a bulking agent to the pre-mixed biomass after a time sufficient for the reagent(s) to have reacted with the biomass. The second stage also includes mixing of the added bulking agent with the pre-mixed biomass to produce a biomass batch suitable for forming a static solid state particle bioreactor. | 12-30-2010 |
20110027858 | Method of Modifying Fungal Morphology - Provided is a method of modifying fungal morphology. The method comprises introducing an amount of a surfactant to a fungus, or to a growing mixture comprising a fungus, wherein said amount of a surfactant is sufficient to induce a change in the morphology of said fungus. | 02-03-2011 |
20110086410 | BGL4 Beta-Glucosidase and Nucleic Acids Encoding the Same - The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same. | 04-14-2011 |
20110201084 | ENZYMATIC HYDROLYSIS OF CELLULOSIC BIOMASS THROUGH ENHANCED REMOVAL OF OLIGOMERS - The methods and composition of the disclosure are directed to the reducing cellulase inhibition by xylooligomers through adding hemicellulase enzymes that break xylooligomers down to monomeric sugars with a corresponding increase in glucose release. What was heretofore unknown is that xylobiose and higher xylooligomers inhibit enzymatic hydrolysis of any lignocellulosic biomass (plant material) used as feed stocks for commercial ethanol production, including, but not limited to, corn stover, birch wood, switchgrass, poplar wood, Miscanthus, aspen, other grasses and woody plants, and many other types of agricultural and forestry residues, as well as municipal solid waste. In addition, the examples herein show that xylobiose and xylotriose have progressively greater effects on hydrolysis rates. In accordance with the disclosure, the addition of one or more hemicellulase enzymes, such as xylanase and β-xylosidase, to a lignocellulosic biomass significantly improves hydrolysis performance. | 08-18-2011 |
20110229956 | Novel Variant Hyprocrea Jecorina CBH1 Cellulases - Described herein are variants of | 09-22-2011 |
20110250674 | CELLULASE VARIANTS - The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:
| 10-13-2011 |
20110256608 | Modified Variant Bowman Birk Protease Inhibitors - The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care. | 10-20-2011 |
20110262997 | PRODUCTION OF CELLULASE - The invention relates to a process of producing cellulase in a host cell, comprising cultivating said host cell capable of producing cellulase under conditions conducive for production of cellulase, wherein pre-treated ligno-cellulosic material is added to induce cellulase production. The invention also relates to use of pre-treated ligno-cellulosic material as inducer or carbon source in cellulase production processes. | 10-27-2011 |
20110269212 | Fungal Endoglucanases, Their Production and Use - Novel fungal endoglucanases with substantial performance at low temperatures are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry or bioethanol production. | 11-03-2011 |
20110269213 | Fungal Endoglucanases, Their Production and Use - Novel fungal endoglucanases Cel5 and Cel2 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production. | 11-03-2011 |
20120015422 | FERMENTATION BROTH FORMULATIONS - The present disclosure relates to fermentation broth formulations containing organic acids and/or organic acid salts, and methods of making and using such formulations. | 01-19-2012 |
20120021490 | GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature. | 01-26-2012 |
20120040435 | Novel Variant Hypocrea Jecorina CBH2 Cellulases - Described herein are variants of | 02-16-2012 |
20120045818 | Plant-Based Production of Heterologous Proteins - Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an | 02-23-2012 |
20120077247 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. | 03-29-2012 |
20120122184 | Polypeptides of strain Bacillus sp. P203 - A new strain | 05-17-2012 |
20120135499 | EXO-ENDO CELLULASE FUSION PROTEIN - The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions. | 05-31-2012 |
20120135500 | CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof. | 05-31-2012 |
20120156754 | VARIANT CBH2 CELLULASES AND RELATED POLYNUCLEOTIDES - The invention provides variants of a | 06-21-2012 |
20120156755 | POLYPEPTIDES HAVING CELLOBIOHYDROLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 06-21-2012 |
20120164709 | RECOMBINANT BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS - The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel. | 06-28-2012 |
20120178144 | METHOD OF PRODUCING CELLULASE - A method is described for producing cellulase by fermentation wherein the fermentation is conducted using | 07-12-2012 |
20120190099 | Methods for Enhancing the Degradation or Conversion of Cellulosic Material - The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material. | 07-26-2012 |
20120202266 | POLYPEPTIDES HAVING ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 08-09-2012 |
20120258520 | Polypeptides having Beta-Glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 10-11-2012 |
20120270298 | NOVEL VARIANT HYPROCREA JECORINA CBH1 CELLULASES - Described herein are variants of | 10-25-2012 |
20120301944 | BGL6 BETA-GLUCOSIDASE AND NUCLEIC ACIDS ENCODING THE SAME - The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same. | 11-29-2012 |
20120315685 | Coated Substrates and Methods of Preparing the Same - Disclosed herein are coated articles and methods of preparing the same. | 12-13-2012 |
20130095554 | MODIFIED FAMILY 5 CELLULASES AND USES THEREOF - The present invention relates to a modified Family 5 cellulase comprising a substitution of an amino acid at position 363 with a non-native alanine, serine or threonine, said position determined from alignment of the modified Family 5 cellulase with amino acids 71-397 of a | 04-18-2013 |
20130102051 | Cellulose Hydrolase and Gene Thereof - A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with | 04-25-2013 |
20130143301 | BETA-GLUCOSIDASE I VARIANTS WITH IMPROVED PROPERTIES - The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants. | 06-06-2013 |
20130177965 | BGL7 Beta-Glucosidase and Nucleic Acids Encoding the Same - The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same. | 07-11-2013 |
20130183739 | Mixed Feedstocks Processing Using an Ionic Liquid - The present invention provides for a composition comprising two or more feedstocks and an ionic liquid (IL). The present invention also provides for a method for treating feedstocks, comprising providing a composition of the present invention comprising two or more feedstocks and an ionic liquid (IL). | 07-18-2013 |
20130189761 | METHOD FOR PRODUCING GLUCOSIDASE, ENZYME COMPOSITION, AND METHOD FOR HYDROLYZING BIOMASS - A method for producing a mutant glucosidase includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. An enzyme composition contains the mutant glucosidase. | 07-25-2013 |
20130210119 | METHOD FOR PRODUCING CELLULOLYTIC AND/OR HEMICELLULOLYTIC ENZYMES - The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%. | 08-15-2013 |
20130323815 | FUNGAL NUCLEIC ACID EXTRACTION - The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment. | 12-05-2013 |
20130337542 | Cellulase Enzyme Mixtures For Depilling and Uses Thereof - The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase enzyme component and one or more additional cellulase enzyme components selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and the Family 5 cellulase enzyme components or the Family 45 and the Family 6 cellulase enzyme components are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing theforegoing cellulase enzyme components. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition. | 12-19-2013 |
20140038262 | NOVEL PAENIBACILLUS SP. HPL-001 STRAIN FOR PRODUCING HIGHLY ACTIVE XYLANASE HAVING HEAT RESISTANCE AND A WIDE PH RANGE, NOVEL XYLANASE ENZYME ISOLATED THEREFROM, AND METHOD FOR MASS-PRODUCING SAME USING A TRANSFORMANT THEREOF - The present invention relates to the novel | 02-06-2014 |
20140051147 | SURFACTANT TOLERANT CELLULASE AND METHOD FOR MODIFICATION THEREOF - A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed. | 02-20-2014 |
20140065693 | Method for treating cellulosic material and CBHII/CEL6A enzymes useful therein - The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations. | 03-06-2014 |
20140093940 | Polypeptides Having Cellulolytic Enhancing Activity And Nucleic Acids Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 04-03-2014 |
20140106434 | APOPLAST WASH FLUID RECOVERY FOR IMPROVED RECOMBINANT ENDOGLUCANASE PRODUCTION IN TOBACCO LEAVES - The present disclosure relates to methods of increasing the production of an apoplast-targeted recombinant protein in a plant tissue and to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue. | 04-17-2014 |
20140113351 | PREPARATION AND USE OF BIOFILM-DEGRADING, MULTIPLE-SPECIFICITY, HYDROLYTIC ENZYME MIXTURES - The present invention relates to isolated structures containing degradative enzymes produced from a marine organism. The enzymes produced are based on the carbon source upon which the marine organism is growing. The enzymes are found in structures that can be isolated such that the degradative enzymes are easily harvested. | 04-24-2014 |
20140113352 | RECOMBINANT THERMOASCUS AURANTIACUS BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS - The present invention provides compositions and methods for the expression of recombinant β-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass. | 04-24-2014 |
20140295523 | Glucanases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures. | 10-02-2014 |
20140295524 | PROCESS FOR THE CONTINUOUS PRODUCTION OF CELLULASES BY A FILAMENTOUS FUNGUS USING A CARBON SUBSTRATE OBTAINED FROM AN ACID PRETREATMENT - A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h | 10-02-2014 |
20140295525 | METHOD FOR PRODUCING CELLULASE AND APPARATUS FOR SAID METHOD - A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentaous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentaous fungi. | 10-02-2014 |
20140302584 | NOVEL VARIANT HYPOCREA JECORINA CBH1 CELLULASE - Described herein are variants of | 10-09-2014 |
20140302585 | CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof. | 10-09-2014 |
20140302586 | Variants of a Family 44 Xyloglucanase - The present invention relates to variants of a parent xyloglucanase. The present invention also relates to polynucleotides encoding the variant xyloglucanases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotide. | 10-09-2014 |
20140302587 | PROCESS FOR PRODUCING CELLULASES USING A FILAMENTOUS FUNGUS SUITABLE FOR A FERMENTER, HAVING A LOW VOLUMETRIC OXYGEN TRANSFER COEFFICIENT KLA - The present invention concerns a process for the production of cellulases using a strain of a filamentous fungus in a stirred, aerated bioreactor, comprising at least two growth steps:
| 10-09-2014 |
20140302588 | VARIANT CBH2 CELLULASES AND RELATED POLYNUCLEOTIDES - The invention provides variants of a | 10-09-2014 |
20140322796 | CELLOBIOHYDROLASE VARIANTS - The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials. | 10-30-2014 |
20140329293 | Polypeptides Having Cellobiohydrolase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 11-06-2014 |
20140349373 | PROCESS FOR THE PRODUCTION OF AN ENZYMATIC COCKTAIL USING SOLID RESIDUES FROM A PROCESS FOR THE BIOCHEMICAL COVERSION OF LIGNOCELLULOSIC MATERIALS - The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases:
| 11-27-2014 |
20150031113 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - Provided are isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides catalytic domains or cellulose binding domains. | 01-29-2015 |
20150064766 | BETA-GLUCOSIDASE VARIANTS - The invention relates to recombinantly produced β-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant β-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target β-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue. | 03-05-2015 |
20150132824 | Polypeptides Having Xanthan Degrading Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xanthan degrading activity, catalytic domains and polynucleotides encoding the polypeptides and catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains. | 05-14-2015 |
20150140638 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 05-21-2015 |
20150292147 | METHODS FOR ENZYMATIC TREATMENT OF WOOL - Disclosed are methods for enzymatic treatment of wool using bacterial protease, cellulase, and xylanase enzymes. | 10-15-2015 |
20150299682 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - Provided are isolated polypeptides having endoglucanase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules. | 10-22-2015 |
20150322418 | RECOMBINANT MESO-ACTIVE THERMO-STABLE PROTEIN AND PROCESSES OF DESIGN AND BIOSYNTHESIS THEREOF - The present invention deals with examination of the alterability of part, or whole, of the surfaces of beta sheet-based protein structures, focusing especially on enzymes. The alteration is done by supplanting/transplanting a part, or whole, of the surface of one protein onto the surface of a homologous protein of superimposable polypeptide backbone, by exploiting the structural features of beta sheets to alter only the regions of the surface involved in substrate/ligand binding and catalysis. The transplantation involves replacement of a selected set of non-contiguous residues constituting the surface regions desired to be altered in one enzyme/protein, by a set of non-contiguous residues located at analogous positions in the other enzyme/protein, in a manner that is likely to facilitate folding and function of the new protein formed by combining residues from both enzymes/proteins. The present invention also deals with using this surface engineering approach to selectively combine enzyme/protein characteristics from different domains of life that are not ordinarily combined by natural evolution, such as the creation of novel proteins that retains the bulk of the thermostable scaffold of a thermophile enzyme onto which the active surface of a mesophile homolog is transplanted, so as to create a thermo-stable protein with meso-active functional characteristics of pH and temperature of optimal function. | 11-12-2015 |
20160024485 | USE OF DIMERIZATION DOMAINS FOR TEMPERATURE REGULATION OF ENZYME ACTIVITY - Methods for regulating protein activity by fusing dimerization domains to target protein are provided. Chimeric proteins that include dimerization domains fused to target proteins for altering the activity of the target proteins are described. Engineered nucleic acids encoding chimeric proteins and hosts engineered to express engineered nucleic acids are also provided. | 01-28-2016 |
20160040144 | Thermostable Trichoderma Cellulase - Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and | 02-11-2016 |
20160068828 | LIQUEFIED CELLULOSIC BIOMASS FOR ENZYME PRODUCTION - Methods for enhancing production of a cellulose component are disclosed herein. | 03-10-2016 |
20160160200 | METHOD FOR PRODUCING HIGHLY ACTIVE CELLOBIOHYDROLASE AND HIGHLY ACTIVE CELLOBIOHYDROLASE - An object of the invention is to provide cellobiohydrolase with high activity. The activity of cellobiohydrolase can be improved by substituting, with alanine, asparagine that is the amino acid at position 62 from the N-terminal of cellobiohydrolase shown in SEQ ID NO: 1, or asparagine at a position corresponding to the position 62 in an amino acid sequence corresponding to the sequence of the cellobiohydrolase shown in SEQ ID NO: 1. | 06-09-2016 |
20160251642 | STABLE FUNGAL CEL6 ENZYME VARIANTS | 09-01-2016 |